MELO3C023903.2 (gene) Melon (DHL92) v3.6.1

BLAST of MELO3C023903.2 vs. NCBI nr
Match: XP_008461721.1 (PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo])

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 722/731 (98.77%), Postives = 722/731 (98.77%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
           MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
           GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
           PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
           ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
           AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL
Sbjct: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360
           TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360

Query: 361 LVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420
           LVSGRDIPNPGFDKSTS         PKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS
Sbjct: 361 LVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420

Query: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480
           NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP
Sbjct: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480

Query: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540
           NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT
Sbjct: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540

Query: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
           YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF
Sbjct: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600

Query: 601 LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP 660
           LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP
Sbjct: 601 LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP 660

Query: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS 720
           QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS
Sbjct: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS 720

Query: 721 VRSPITITSLV 725
           VRSPITITSLV
Sbjct: 721 VRSPITITSLV 731

BLAST of MELO3C023903.2 vs. NCBI nr
Match: Q39547.1 (RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor >BAA06905.1 pre-pro-cucumisin [Cucumis melo])

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 716/731 (97.95%), Postives = 718/731 (98.22%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
           MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
           GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
           PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
           ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
           AYKVCWNDGCSD DILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360
           TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360

Query: 361 LVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420
           LVSGRDIPN GFDKSTS        NP LLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS
Sbjct: 361 LVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420

Query: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480
           NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP
Sbjct: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480

Query: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540
           NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT
Sbjct: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540

Query: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
           YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF
Sbjct: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600

Query: 601 LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP 660
           LCGQGYNT+AVRRITGDYSACT GNTGRVWDLNYPSFGLSVSPS+TFNQYFNRTLTSVAP
Sbjct: 601 LCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAP 660

Query: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS 720
           QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVH 
Sbjct: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHY 720

Query: 721 VRSPITITSLV 725
           VRSPITITSLV
Sbjct: 721 VRSPITITSLV 731

BLAST of MELO3C023903.2 vs. NCBI nr
Match: 3VTA_A (Chain A, Crystal Structure Of Cucumisin, A Subtilisin-like Endoprotease From Cucumis Melo L >3VTA_B Chain B, Crystal Structure Of Cucumisin, A Subtilisin-like Endoprotease From Cucumis Melo L >4YN3_A Chain A, Crystal Structure Of Cucumisin Complex With Pro-peptide)

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 606/621 (97.58%), Postives = 608/621 (97.91%), Query Frame = 0

Query: 111 TTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSN 170
           TTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSN
Sbjct: 1   TTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSN 60

Query: 171 NFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTA 230
           NFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTA
Sbjct: 61  NFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTA 120

Query: 231 RGGVPLARIAAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIG 290
           RGGVPLARIAAYKVCWNDGCSD DILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIG
Sbjct: 121 RGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIG 180

Query: 291 SFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVS 350
           SFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVS
Sbjct: 181 SFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVS 240

Query: 351 INTFDNQYYPLVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSL 410
           INTFDNQYYPLVSGRDIPN GFDKSTS        NP LLKGKIVVCEASFGPHEFFKSL
Sbjct: 241 INTFDNQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSL 300

Query: 411 DGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAP 470
           DGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAP
Sbjct: 301 DGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAP 360

Query: 471 VVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPH 530
           VVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPH
Sbjct: 361 VVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPH 420

Query: 531 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 590
           ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY
Sbjct: 421 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 480

Query: 591 DANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQY 650
           DANESDYVKFLCGQGYNT+AVRRITGDYSACT GNTGRVWDLNYPSFGLSVSPS+TFNQY
Sbjct: 481 DANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQY 540

Query: 651 FNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSA 710
           FNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSA
Sbjct: 541 FNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSA 600

Query: 711 SLVWSDGVHSVRSPITITSLV 725
           SLVWSDGVH VRSPITITSLV
Sbjct: 601 SLVWSDGVHYVRSPITITSLV 621

BLAST of MELO3C023903.2 vs. NCBI nr
Match: XP_008461722.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1065.1 bits (2753), Expect = 1.1e-307
Identity = 553/733 (75.44%), Postives = 607/733 (82.81%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRK-LEDPDSAHLHHRAMLEQV 60
           MS SLI KL FF+LFF   LAS LDSDD  K IYIVYMG+K  +DPD A+LHH +     
Sbjct: 7   MSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHSSF---- 66

Query: 61  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
               FAPESVL+TY RSFNGFAVKLT+EEA+KIASMEGVVSVF NEMN  HTTRSWDF+G
Sbjct: 67  ---PFAPESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMG 126

Query: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
           F   VPR  QVESN+VVGVLD+GIWPESPSF+D+GF PPP KWKGTC ++ NF CNRKII
Sbjct: 127 FSQNVPRVKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTC-SAINFTCNRKII 186

Query: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
           GARSYHIGRP+  GDV GPRDTNGHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARI
Sbjct: 187 GARSYHIGRPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARI 246

Query: 241 AAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
           A YKVCW D CSDADILAA+DDAIADGVDIISLSVG    R YF+D+IAIGSFHA+E+GI
Sbjct: 247 AVYKVCWRDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGI 306

Query: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ-Y 360
           LTSNSAGN GP   TTASLSPWLLSVAAST+DRKFVT+VQIGN  SFQG SINTFDN   
Sbjct: 307 LTSNSAGNNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQ 366

Query: 361 YPLVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
           YPLV+GR +PN GFD + SS       + KL+KGKI++CEA+F   + F +L G AGVLM
Sbjct: 367 YPLVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDA-KHFVTLGGVAGVLM 426

Query: 421 -TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480
             +   D A SYP+PS++LD ND +AT RYIYS  SP ATIFKST   N  APVVVSFSS
Sbjct: 427 IDTELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSS 486

Query: 481 RGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATY 540
           RGPN  TK++IKPD+SGPGVEILAAWP VA VGGI RNTL+NI+SGTSMSCPHITGIA Y
Sbjct: 487 RGPNNITKEIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAY 546

Query: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
           VKT+NPTWSPAAIKSALMTTA PMNA  N  AEFAYG+GHVNPLKAVRPGLVYDANESDY
Sbjct: 547 VKTFNPTWSPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDY 606

Query: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660
           VKFLCGQGY T  VR IT D SACT  N GRVWDLNYPSFGLSVS S+TFNQYF R LT+
Sbjct: 607 VKFLCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTN 666

Query: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
           VA QASTYRA IS+PQGLTI+VNP VLSFNG+GDRKSFTLTV+G+IK  VVSASLVW DG
Sbjct: 667 VASQASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDG 726

Query: 721 VHSVRSPITITSL 724
           VHSVRSPIT+TSL
Sbjct: 727 VHSVRSPITVTSL 728

BLAST of MELO3C023903.2 vs. NCBI nr
Match: XP_022957348.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1026.5 bits (2653), Expect = 4.2e-296
Identity = 523/733 (71.35%), Postives = 595/733 (81.17%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
           MSS LI  L F +LF S  LAS   S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 8   MSSPLILNLLFLALFCS-VLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRAMLEQVV 67

Query: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
           GSTF P+SVL+TY RSFNGF V LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDF+GF
Sbjct: 68  GSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGF 127

Query: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
           P  V R  Q+ESNIVVGVLD+GIWPESPSF+D+GF  PP KW+G C+ S NF CNRK+IG
Sbjct: 128 PQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKLIG 187

Query: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
            R+YHIGRPI+PGDV  PRDT+GHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARIA
Sbjct: 188 GRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIA 247

Query: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
           AYKVCW DGC DADILAA+DDAIADGVDI+SLSVG   PR YF D IAIGSFHA++ GIL
Sbjct: 248 AYKVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAIKNGIL 307

Query: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQYY 360
           TSNSAGN GP  FTT SLSPWLLSVAAS MDRKFVT+VQ+GNG  +QG+SINTF+ N  +
Sbjct: 308 TSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQH 367

Query: 361 PLVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT 420
           PLV   DIPN GF+ STS        NP L  GKI++C++   P  FF S     G+LM 
Sbjct: 368 PLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFF-SFGAIVGILMQ 427

Query: 421 SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRG 480
           + TRD A SYP P++VLDPND +A  RYI S   P ATIFKS    ++SAPVVVSFSSRG
Sbjct: 428 AYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRG 487

Query: 481 PNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIATY 540
           PN  T D++KPD+SGPGVEILAAWP VAP+ G++  R++L+NIISGTSMSCPH+TG+A Y
Sbjct: 488 PNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVY 547

Query: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
           VKT+NPT SPAAIKSALMTTASPM+AR + QAEFAYG+GHVNPLKA+ PGLVYDANESDY
Sbjct: 548 VKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDY 607

Query: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660
           V FLCGQGY+T  VRRITGD SACT GN GRVWDLNYPSF +S+S ++T NQYF RTLT+
Sbjct: 608 VSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTN 667

Query: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
           VA   STYRA ISAPQGL+I+VNP  LSFNG+GDRKSF LTVRG++   + SASL WSDG
Sbjct: 668 VARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISSASLEWSDG 727

Query: 721 VHSVRSPITITSL 724
           VHSVRSPIT+ +L
Sbjct: 728 VHSVRSPITVVAL 737

BLAST of MELO3C023903.2 vs. TAIR10
Match: AT5G59090.1 (subtilase 4.12)

HSP 1 Score: 620.2 bits (1598), Expect = 1.7e-177
Identity = 335/717 (46.72%), Postives = 462/717 (64.44%), Query Frame = 0

Query: 28  DDGKNIYIVYMGRKLEDPDSAHL-HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTE 87
           D+   +YIVYMG      D      H ++L+QV G +     ++ +YKRSFNGFA +LTE
Sbjct: 27  DEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTE 86

Query: 88  EEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP--LTVPRRSQVESNIVVGVLDTGIW 147
            E   IA +EGVVSVF N++ +LHTT SWDF+G        R   +ES+ ++GV+DTGIW
Sbjct: 87  SERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIW 146

Query: 148 PESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGH 207
           PES SF D+GF PPP KWKG C    NF CN K+IGAR Y            G RDT+GH
Sbjct: 147 PESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TSEGTRDTSGH 206

Query: 208 GTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDADILAAYDDAIA 267
           GTHTASTAAG  V   + +G+G GT RGGVP +RIAAYKVC + GCS   +L+++DDAIA
Sbjct: 207 GTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIA 266

Query: 268 DGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLS 327
           DGVD+I++S+G   P  +  D IAIG+FHA+ +GILT +SAGN GP   T + ++PW+ +
Sbjct: 267 DGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFT 326

Query: 328 VAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNPGFDKSTSS---- 387
           VAAST +R F+T+V +GNG++  G S+N FD   + YPLV G+   +   D  T++    
Sbjct: 327 VAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAP 386

Query: 388 ---NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLL 447
              N   +KGKI+VC    G ++  KS+ GA  ++  S   D A ++ LP+S L   D  
Sbjct: 387 ACLNKSRVKGKILVCGGPSG-YKIAKSV-GAIAIIDKSPRPDVAFTHHLPASGLKAKDFK 446

Query: 448 ATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAA 507
           + + YI S  SP A + K+ TI N ++PV+ SFSSRGPN    D++KPDI+ PGVEILAA
Sbjct: 447 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 506

Query: 508 W-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPM 567
           + P+  P     R   +++ SGTSM+CPH+ G+A YVKT+ P WSP+ I+SA+MTTA P+
Sbjct: 507 FSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPV 566

Query: 568 NA--RFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYS 627
            A  R     EFAYG+GHV+P+ A+ PGLVY+ +++D++ FLCG  Y ++ ++ I+GD  
Sbjct: 567 KAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTV 626

Query: 628 ACTPGNTGRVWDLNYPSFGLSVS-PSKTFNQYFNRTLTSVAPQASTYRAMISAPQG--LT 687
            C+  N     +LNYPS    +S    TF+  FNRTLT+V    STY++ + A  G  L+
Sbjct: 627 KCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 686

Query: 688 ISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVV--SASLVWSDGVHSVRSPITITSLV 725
           I V P+VL F  + +++SF++TV GS     V  SA+L+WSDG H+VRSPI +  +V
Sbjct: 687 IKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIMV 732

BLAST of MELO3C023903.2 vs. TAIR10
Match: AT3G46850.1 (Subtilase family protein)

HSP 1 Score: 617.1 bits (1590), Expect = 1.4e-176
Identity = 341/715 (47.69%), Postives = 453/715 (63.36%), Query Frame = 0

Query: 27  DDDGKNIYIVYMGRKLEDPDSAHL-HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLT 86
           DD  K +YIVYMG      D   + HH ++L+ V G +   + ++  YKRSFNGFA +LT
Sbjct: 27  DDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLT 86

Query: 87  EEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP--LTVPRRSQVESNIVVGVLDTGI 146
           E E E +ASM+ VVSVF ++   L TT SW+F+G        R   +ES+ ++GV+D+GI
Sbjct: 87  ESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGI 146

Query: 147 WPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNG 206
           +PES SF  +GF PPP KWKG C+   NF CN K+IGAR Y    P   G     RD  G
Sbjct: 147 YPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY---TPKLEGFPESARDNTG 206

Query: 207 HGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDG---CSDADILAAYD 266
           HG+HTAS AAG  V   + YGLG GT RGGVP ARIA YKVC + G   C+   ILAA+D
Sbjct: 207 HGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVC-DPGVIRCTSDGILAAFD 266

Query: 267 DAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSP 326
           DAIAD VDII++S+G      +  D +AIG+FHA+ +GILT N AGN GP   T  S++P
Sbjct: 267 DAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAP 326

Query: 327 WLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGR------DIPNPGF 386
           WL +VAAS M+R F+T+V +GNG++  G S+N+FD   + YPLV G+      D  + GF
Sbjct: 327 WLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSASSRCDASSAGF 386

Query: 387 DKSTSSNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPN 446
                 + K +KGKIV+C+    P E      GA   ++ +   D A  +  P SVL  +
Sbjct: 387 CSPGCLDSKRVKGKIVLCDTQRNPGE--AQAMGAVASIVRNPYEDAASVFSFPVSVLSED 446

Query: 447 DLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEI 506
           D    L Y+ S ++P A + KS TI N  APVV S+SSRGPN    D++KPDI+ PG EI
Sbjct: 447 DYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEI 506

Query: 507 LAAW-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTA 566
           LAA+ P V P     R+  + +ISGTSMSCPH+ G+A Y+KT++P WSP+ I+SA+MTTA
Sbjct: 507 LAAYSPYVPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTA 566

Query: 567 SPMNARFNPQ---AEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRIT 626
            PMNA  +P    AEFAYG+GHV+P+ A+ PGLVY+AN+SD++ FLCG  Y  + +R I+
Sbjct: 567 WPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLIS 626

Query: 627 GDYSACTPGNTGRVW-DLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQG 686
           GD S+CT   T  +  +LNYPS    VS +K F   F RT+T+V    +TY+A +   + 
Sbjct: 627 GDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK- 686

Query: 687 LTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHSVRSPITI 721
           L + V P VLS   L ++KSFT+TV G+      +VSA L+WSDGVH VRSPI +
Sbjct: 687 LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

BLAST of MELO3C023903.2 vs. TAIR10
Match: AT5G59190.1 (subtilase family protein)

HSP 1 Score: 611.7 bits (1576), Expect = 5.9e-175
Identity = 331/703 (47.08%), Postives = 460/703 (65.43%), Query Frame = 0

Query: 38  MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97
           MG   E   S   HH ++L+++VG+  A   ++ +YKRSFNGFA  L++ E++K+ +M+ 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 98  VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157
           VVSVF ++ +EL TTRSWDF+GF     R S  ES+++VGV+D+GIWPES SFDDEGF P
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 158 PPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLV 217
           PP KWKG+C+    F CN K+IGAR Y+          +  RD  GHGTHTASTAAG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180

Query: 218 SQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGA 277
             A+ YGL  GTARGGVP ARIAAYKVC+N  C+D DILAA+DDAIADGVD+IS+S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 278 NPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQ 337
              +    ++AIGSFHA+ RGI+T+ SAGN GP+  + A++SPW+++VAAS  DR+F+ +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 338 VQIGNGQSFQGVSINTF--DNQYYPLVSGRDI------PNPGFDKSTSSNPKLLKGKIVV 397
           V +GNG++  G+S+NTF  +   +P+V G+++         G+  S   + +L+KGKIV+
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVL 360

Query: 398 CEASFGPHEFFKSLDGAAGVLMTSN-TRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPG 457
           C+   G  E +  L GA GV++ +    D A   P P+S L   D  +   YI S   P 
Sbjct: 361 CDDFLGYREAY--LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQ 420

Query: 458 ATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI--- 517
           A I ++  I++  AP V SFSSRGP+   ++++KPD+S PG+EILAA+  VA        
Sbjct: 421 AEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNP 480

Query: 518 --RRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAE 577
             +R+  ++++SGTSM+CPH+ G+A YVK+++P WSP+AIKSA+MTTA+PMN + NP+ E
Sbjct: 481 EDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQE 540

Query: 578 FAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVW 637
           FAYGSG +NP KA  PGLVY+    DY+K LC +G+++  +   +G    C+      V 
Sbjct: 541 FAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS--ERTEVK 600

Query: 638 DLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQ-GLTISVNPNVLSFNGL 697
           DLNYP+    VS    FN  F RT+T+V    STY+A +   Q  L IS+ P +L F  L
Sbjct: 601 DLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFL 660

Query: 698 GDRKSFTLTVRGS--IKGFVVSASLVWSDGVHSVRSPITITSL 724
            ++KSF +T+ G     G  VS+S+VWSDG HSVRSPI   S+
Sbjct: 661 EEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of MELO3C023903.2 vs. TAIR10
Match: AT3G46840.1 (Subtilase family protein)

HSP 1 Score: 611.3 bits (1575), Expect = 7.7e-175
Identity = 341/716 (47.63%), Postives = 456/716 (63.69%), Query Frame = 0

Query: 25  DSDDDGKNIYIVYMGRKLEDPDSAHL-HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVK 84
           D DD  K  YIVYMG      D   + HH ++L+ V G +   + ++  YKRSFNGFA +
Sbjct: 26  DKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDRLVRNYKRSFNGFAAR 85

Query: 85  LTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLT--VPRRSQVESNIVVGVLDT 144
           LT+ E E +ASM+ VVSVF N+  +L TT SW+F+G   +    R + +ES+ ++GV+D+
Sbjct: 86  LTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDS 145

Query: 145 GIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDT 204
           GI+PES SF  +GF PPP KWKG C+   NF  N K+IGAR Y    P   G     RD 
Sbjct: 146 GIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY---TPKLEGFPESARDY 205

Query: 205 NGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVC--WNDGCSDADILAAY 264
            GHG+HTASTAAG  V   + YGLG GTARGGVP ARIA YKVC    DGC+   ILAA+
Sbjct: 206 MGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDGCTTDGILAAF 265

Query: 265 DDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLS 324
           DDAIAD VDII++S+GG N   +  D IAIG+FHA+ +GIL  NSAGN GP   T AS++
Sbjct: 266 DDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIA 325

Query: 325 PWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRD------IPNPG 384
           PW+ +VAAS  +R FVT+V +GNG++  G S+N+FD   + YPLV G+         + G
Sbjct: 326 PWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGKSASSSCGAASAG 385

Query: 385 FDKSTSSNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDP 444
           F      + K +KGKIV+C++   P E      GA   ++ S+  D A  +  P SVL  
Sbjct: 386 FCSPGCLDSKRVKGKIVLCDSPQNPDE--AQAMGAIASIVRSHRTDVASIFSFPVSVLLE 445

Query: 445 NDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVE 504
           +D    L Y+ S ++P A + KS TI N  APVV S+ SRGPN    D++KPDI+ PG E
Sbjct: 446 DDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSE 505

Query: 505 ILAAW-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTT 564
           I+AA+ P   P     R   +++ +GTSMSCPH+ G+A Y+K+++P WSP+ I+SA+MTT
Sbjct: 506 IVAAYSPDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTT 565

Query: 565 ASPMNAR---FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRI 624
           A PMNA    FN  AEFAYG+GHV+P+ A+ PGLVY+AN+SD++ FLCG  Y  + +R I
Sbjct: 566 AWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLI 625

Query: 625 TGDYSACTPGNTGRV-WDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQ 684
           +GD S+CT   T  +  +LNYPS    VS +K F   F RT+T+V    +TY+A +   +
Sbjct: 626 SGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK 685

Query: 685 GLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHSVRSPITI 721
            L + V P VLS   L ++KSFT+T  G+      +VSA L+WSDGVH VRSPI +
Sbjct: 686 -LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

BLAST of MELO3C023903.2 vs. TAIR10
Match: AT5G59120.1 (subtilase 4.13)

HSP 1 Score: 611.3 bits (1575), Expect = 7.7e-175
Identity = 332/722 (45.98%), Postives = 463/722 (64.13%), Query Frame = 0

Query: 22  SRLDSDDDGKNIYIVYMG----RKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSF 81
           S + +  D K +YIVYMG    R    P S H++   +L++V G +     ++ +YKRSF
Sbjct: 20  SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMN---ILQEVTGESSIEGRLVRSYKRSF 79

Query: 82  NGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP--LTVPRRSQVESNIV 141
           NGFA +LTE E E++A M GVVSVF N+  +L TT SWDF+G    +   R   VES+ +
Sbjct: 80  NGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTI 139

Query: 142 VGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDV 201
           +GV+D+GI PES SF D+GF PPP KWKG C    NF CN K+IGAR Y           
Sbjct: 140 IGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDY---------TS 199

Query: 202 NGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDADI 261
            G RD +GHGTHTASTAAG  V  A+ +G+G GT RGGVP +R+AAYKVC   GCS   +
Sbjct: 200 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 259

Query: 262 LAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTT 321
           L+A+DDAIADGVD+I++S+G      +  D IAIG+FHA+ +G+LT NSAGN GP   + 
Sbjct: 260 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 319

Query: 322 ASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGR-------D 381
           + ++PW+L+VAAST +R FVT+V +GNG++  G S+N ++   + YPLV G+       D
Sbjct: 320 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 379

Query: 382 IPNPGFDKSTSSNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPS 441
             + G  + +  +   +KGKI+VC    G  +  +S+ GA G++  +   D A  +PLP+
Sbjct: 380 AESAGLCELSCVDKSRVKGKILVCGGP-GGLKIVESV-GAVGLIYRTPKPDVAFIHPLPA 439

Query: 442 SVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDIS 501
           + L   D  + + Y+ S  SP A + K+  I N ++PV+ SFSSRGPN    D++KPDI+
Sbjct: 440 AGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDIT 499

Query: 502 GPGVEILAAW-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKS 561
            PGVEILAA+ P+  P     R+  ++++SGTSMSCPH+ G+A YVKT+NP WSP+ I+S
Sbjct: 500 APGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQS 559

Query: 562 ALMTTASPMNARFN--PQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEA 621
           A+MTTA P+NA        EFAYGSGHV+P+ A  PGLVY+ ++SD++ FLCG  Y ++ 
Sbjct: 560 AIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQV 619

Query: 622 VRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPS-KTFNQYFNRTLTSVAPQASTYRAMI 681
           ++ I+G+   C+        +LNYPS    +S S  TF   FNRTLT+V    STY + +
Sbjct: 620 LKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKV 679

Query: 682 SAPQG--LTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVV--SASLVWSDGVHSVRSPI 721
            A  G  L + + P+VLSF  + +++SFT+TV GS     V  SA+L+WSDG H+VRSPI
Sbjct: 680 VAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPI 727

BLAST of MELO3C023903.2 vs. Swiss-Prot
Match: sp|Q39547|CUCM1_CUCME (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 716/731 (97.95%), Postives = 718/731 (98.22%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
           MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
           GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
           PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
           ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
           AYKVCWNDGCSD DILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360
           TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360

Query: 361 LVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420
           LVSGRDIPN GFDKSTS        NP LLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS
Sbjct: 361 LVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420

Query: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480
           NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP
Sbjct: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480

Query: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540
           NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT
Sbjct: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540

Query: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
           YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF
Sbjct: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600

Query: 601 LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP 660
           LCGQGYNT+AVRRITGDYSACT GNTGRVWDLNYPSFGLSVSPS+TFNQYFNRTLTSVAP
Sbjct: 601 LCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAP 660

Query: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS 720
           QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVH 
Sbjct: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHY 720

Query: 721 VRSPITITSLV 725
           VRSPITITSLV
Sbjct: 721 VRSPITITSLV 731

BLAST of MELO3C023903.2 vs. Swiss-Prot
Match: sp|Q9FIF8|SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 7.9e-177
Identity = 335/709 (47.25%), Postives = 466/709 (65.73%), Query Frame = 0

Query: 32  NIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEK 91
           ++YIVYMG   E   S   HH ++L+++VG+  A   ++ +YKRSFNGFA  L++ E++K
Sbjct: 31  SVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQK 90

Query: 92  IASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFD 151
           + +M+ VVSVF ++ +EL TTRSWDF+GF     R S  ES+++VGV+D+GIWPES SFD
Sbjct: 91  LQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFD 150

Query: 152 DEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTAST 211
           DEGF PPP KWKG+C+    F CN K+IGAR Y+          +  RD  GHGTHTAST
Sbjct: 151 DEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTAST 210

Query: 212 AAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDADILAAYDDAIADGVDIIS 271
           AAG  V  A+ YGL  GTARGGVP ARIAAYKVC+N  C+D DILAA+DDAIADGVD+IS
Sbjct: 211 AAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDDAIADGVDVIS 270

Query: 272 LSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMD 331
           +S+      +    ++AIGSFHA+ RGI+T+ SAGN GP+  + A++SPW+++VAAS  D
Sbjct: 271 ISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTD 330

Query: 332 RKFVTQVQIGNGQSFQGVSINTF--DNQYYPLVSGRDI------PNPGFDKSTSSNPKLL 391
           R+F+ +V +GNG++  G+S+NTF  +   +P+V G+++         G+  S   + +L+
Sbjct: 331 RQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELV 390

Query: 392 KGKIVVCEASFGPHEFFKSLDGAAGVLMTSN-TRDYADSYPLPSSVLDPNDLLATLRYIY 451
           KGKIV+C+   G  E +  L GA GV++ +    D A   P P+S L   D  +   YI 
Sbjct: 391 KGKIVLCDDFLGYREAY--LAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIE 450

Query: 452 SIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV 511
           S   P A I ++  I++  AP V SFSSRGP+   ++++KPD+S PG+EILAA+  VA  
Sbjct: 451 SAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASP 510

Query: 512 GGI-----RRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNAR 571
                   +R+  ++++SGTSM+CPH+ G+A YVK+++P WSP+AIKSA+MTTA+PMN +
Sbjct: 511 SSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLK 570

Query: 572 FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPG 631
            NP+ EFAYGSG +NP KA  PGLVY+    DY+K LC +G+++  +   +G    C+  
Sbjct: 571 KNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS-- 630

Query: 632 NTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQ-GLTISVNPNV 691
               V DLNYP+    VS    FN  F RT+T+V    STY+A +   Q  L IS+ P +
Sbjct: 631 ERTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEI 690

Query: 692 LSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHSVRSPITITSL 724
           L F  L ++KSF +T+ G     G  VS+S+VWSDG HSVRSPI   S+
Sbjct: 691 LRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of MELO3C023903.2 vs. Swiss-Prot
Match: sp|Q8L7D2|SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 3.0e-176
Identity = 335/717 (46.72%), Postives = 462/717 (64.44%), Query Frame = 0

Query: 28  DDGKNIYIVYMGRKLEDPDSAHL-HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTE 87
           D+   +YIVYMG      D      H ++L+QV G +     ++ +YKRSFNGFA +LTE
Sbjct: 27  DEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSYKRSFNGFAARLTE 86

Query: 88  EEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP--LTVPRRSQVESNIVVGVLDTGIW 147
            E   IA +EGVVSVF N++ +LHTT SWDF+G        R   +ES+ ++GV+DTGIW
Sbjct: 87  SERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIESDTIIGVIDTGIW 146

Query: 148 PESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGH 207
           PES SF D+GF PPP KWKG C    NF CN K+IGAR Y            G RDT+GH
Sbjct: 147 PESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY---------TSEGTRDTSGH 206

Query: 208 GTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDADILAAYDDAIA 267
           GTHTASTAAG  V   + +G+G GT RGGVP +RIAAYKVC + GCS   +L+++DDAIA
Sbjct: 207 GTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLSSFDDAIA 266

Query: 268 DGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLS 327
           DGVD+I++S+G   P  +  D IAIG+FHA+ +GILT +SAGN GP   T + ++PW+ +
Sbjct: 267 DGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFT 326

Query: 328 VAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNPGFDKSTSS---- 387
           VAAST +R F+T+V +GNG++  G S+N FD   + YPLV G+   +   D  T++    
Sbjct: 327 VAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACDAKTAALCAP 386

Query: 388 ---NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLL 447
              N   +KGKI+VC    G ++  KS+ GA  ++  S   D A ++ LP+S L   D  
Sbjct: 387 ACLNKSRVKGKILVCGGPSG-YKIAKSV-GAIAIIDKSPRPDVAFTHHLPASGLKAKDFK 446

Query: 448 ATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAA 507
           + + YI S  SP A + K+ TI N ++PV+ SFSSRGPN    D++KPDI+ PGVEILAA
Sbjct: 447 SLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAA 506

Query: 508 W-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPM 567
           + P+  P     R   +++ SGTSM+CPH+ G+A YVKT+ P WSP+ I+SA+MTTA P+
Sbjct: 507 FSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPV 566

Query: 568 NA--RFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYS 627
            A  R     EFAYG+GHV+P+ A+ PGLVY+ +++D++ FLCG  Y ++ ++ I+GD  
Sbjct: 567 KAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTV 626

Query: 628 ACTPGNTGRVWDLNYPSFGLSVS-PSKTFNQYFNRTLTSVAPQASTYRAMISAPQG--LT 687
            C+  N     +LNYPS    +S    TF+  FNRTLT+V    STY++ + A  G  L+
Sbjct: 627 KCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLS 686

Query: 688 ISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVV--SASLVWSDGVHSVRSPITITSLV 725
           I V P+VL F  + +++SF++TV GS     V  SA+L+WSDG H+VRSPI +  +V
Sbjct: 687 IKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIMV 732

BLAST of MELO3C023903.2 vs. Swiss-Prot
Match: sp|Q9STF7|SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.5e-175
Identity = 341/715 (47.69%), Postives = 453/715 (63.36%), Query Frame = 0

Query: 27  DDDGKNIYIVYMGRKLEDPDSAHL-HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLT 86
           DD  K +YIVYMG      D   + HH ++L+ V G +   + ++  YKRSFNGFA +LT
Sbjct: 27  DDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLT 86

Query: 87  EEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP--LTVPRRSQVESNIVVGVLDTGI 146
           E E E +ASM+ VVSVF ++   L TT SW+F+G        R   +ES+ ++GV+D+GI
Sbjct: 87  ESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGI 146

Query: 147 WPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNG 206
           +PES SF  +GF PPP KWKG C+   NF CN K+IGAR Y    P   G     RD  G
Sbjct: 147 YPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYY---TPKLEGFPESARDNTG 206

Query: 207 HGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDG---CSDADILAAYD 266
           HG+HTAS AAG  V   + YGLG GT RGGVP ARIA YKVC + G   C+   ILAA+D
Sbjct: 207 HGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVC-DPGVIRCTSDGILAAFD 266

Query: 267 DAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSP 326
           DAIAD VDII++S+G      +  D +AIG+FHA+ +GILT N AGN GP   T  S++P
Sbjct: 267 DAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAP 326

Query: 327 WLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGR------DIPNPGF 386
           WL +VAAS M+R F+T+V +GNG++  G S+N+FD   + YPLV G+      D  + GF
Sbjct: 327 WLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSASSRCDASSAGF 386

Query: 387 DKSTSSNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPN 446
                 + K +KGKIV+C+    P E      GA   ++ +   D A  +  P SVL  +
Sbjct: 387 CSPGCLDSKRVKGKIVLCDTQRNPGE--AQAMGAVASIVRNPYEDAASVFSFPVSVLSED 446

Query: 447 DLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEI 506
           D    L Y+ S ++P A + KS TI N  APVV S+SSRGPN    D++KPDI+ PG EI
Sbjct: 447 DYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEI 506

Query: 507 LAAW-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTA 566
           LAA+ P V P     R+  + +ISGTSMSCPH+ G+A Y+KT++P WSP+ I+SA+MTTA
Sbjct: 507 LAAYSPYVPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTA 566

Query: 567 SPMNARFNPQ---AEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRIT 626
            PMNA  +P    AEFAYG+GHV+P+ A+ PGLVY+AN+SD++ FLCG  Y  + +R I+
Sbjct: 567 WPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLIS 626

Query: 627 GDYSACTPGNTGRVW-DLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQG 686
           GD S+CT   T  +  +LNYPS    VS +K F   F RT+T+V    +TY+A +   + 
Sbjct: 627 GDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK- 686

Query: 687 LTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHSVRSPITI 721
           L + V P VLS   L ++KSFT+TV G+      +VSA L+WSDGVH VRSPI +
Sbjct: 687 LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

BLAST of MELO3C023903.2 vs. Swiss-Prot
Match: sp|F4JA91|SBT45_ARATH (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.4e-173
Identity = 341/716 (47.63%), Postives = 456/716 (63.69%), Query Frame = 0

Query: 25  DSDDDGKNIYIVYMGRKLEDPDSAHL-HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVK 84
           D DD  K  YIVYMG      D   + HH ++L+ V G +   + ++  YKRSFNGFA +
Sbjct: 26  DKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSIEDRLVRNYKRSFNGFAAR 85

Query: 85  LTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLT--VPRRSQVESNIVVGVLDT 144
           LT+ E E +ASM+ VVSVF N+  +L TT SW+F+G   +    R + +ES+ ++GV+D+
Sbjct: 86  LTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDS 145

Query: 145 GIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDT 204
           GI+PES SF  +GF PPP KWKG C+   NF  N K+IGAR Y    P   G     RD 
Sbjct: 146 GIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY---TPKLEGFPESARDY 205

Query: 205 NGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVC--WNDGCSDADILAAY 264
            GHG+HTASTAAG  V   + YGLG GTARGGVP ARIA YKVC    DGC+   ILAA+
Sbjct: 206 MGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDPGVDGCTTDGILAAF 265

Query: 265 DDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLS 324
           DDAIAD VDII++S+GG N   +  D IAIG+FHA+ +GIL  NSAGN GP   T AS++
Sbjct: 266 DDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIA 325

Query: 325 PWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRD------IPNPG 384
           PW+ +VAAS  +R FVT+V +GNG++  G S+N+FD   + YPLV G+         + G
Sbjct: 326 PWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVYGKSASSSCGAASAG 385

Query: 385 FDKSTSSNPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDP 444
           F      + K +KGKIV+C++   P E      GA   ++ S+  D A  +  P SVL  
Sbjct: 386 FCSPGCLDSKRVKGKIVLCDSPQNPDE--AQAMGAIASIVRSHRTDVASIFSFPVSVLLE 445

Query: 445 NDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVE 504
           +D    L Y+ S ++P A + KS TI N  APVV S+ SRGPN    D++KPDI+ PG E
Sbjct: 446 DDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSE 505

Query: 505 ILAAW-PSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTT 564
           I+AA+ P   P     R   +++ +GTSMSCPH+ G+A Y+K+++P WSP+ I+SA+MTT
Sbjct: 506 IVAAYSPDAPPSISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTT 565

Query: 565 ASPMNAR---FNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRI 624
           A PMNA    FN  AEFAYG+GHV+P+ A+ PGLVY+AN+SD++ FLCG  Y  + +R I
Sbjct: 566 AWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLI 625

Query: 625 TGDYSACTPGNTGRV-WDLNYPSFGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQ 684
           +GD S+CT   T  +  +LNYPS    VS +K F   F RT+T+V    +TY+A +   +
Sbjct: 626 SGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK 685

Query: 685 GLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHSVRSPITI 721
            L + V P VLS   L ++KSFT+T  G+      +VSA L+WSDGVH VRSPI +
Sbjct: 686 -LKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

BLAST of MELO3C023903.2 vs. TrEMBL
Match: tr|A0A1S3CF86|A0A1S3CF86_CUCME (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=4 SV=1)

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 722/731 (98.77%), Postives = 722/731 (98.77%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
           MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
           GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
           PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
           ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
           AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL
Sbjct: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360
           TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQYYP 360

Query: 361 LVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420
           LVSGRDIPNPGFDKSTS         PKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS
Sbjct: 361 LVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTS 420

Query: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480
           NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP
Sbjct: 421 NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGP 480

Query: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540
           NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT
Sbjct: 481 NRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATYVKT 540

Query: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600
           YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF
Sbjct: 541 YNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKF 600

Query: 601 LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP 660
           LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP
Sbjct: 601 LCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVAP 660

Query: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS 720
           QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS
Sbjct: 661 QASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHS 720

Query: 721 VRSPITITSLV 725
           VRSPITITSLV
Sbjct: 721 VRSPITITSLV 731

BLAST of MELO3C023903.2 vs. TrEMBL
Match: tr|A0A1S3CFD6|A0A1S3CFD6_CUCME (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=4 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 7.1e-308
Identity = 553/733 (75.44%), Postives = 607/733 (82.81%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRK-LEDPDSAHLHHRAMLEQV 60
           MS SLI KL FF+LFF   LAS LDSDD  K IYIVYMG+K  +DPD A+LHH +     
Sbjct: 7   MSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHSSF---- 66

Query: 61  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
               FAPESVL+TY RSFNGFAVKLT+EEA+KIASMEGVVSVF NEMN  HTTRSWDF+G
Sbjct: 67  ---PFAPESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMG 126

Query: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
           F   VPR  QVESN+VVGVLD+GIWPESPSF+D+GF PPP KWKGTC ++ NF CNRKII
Sbjct: 127 FSQNVPRVKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTC-SAINFTCNRKII 186

Query: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
           GARSYHIGRP+  GDV GPRDTNGHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARI
Sbjct: 187 GARSYHIGRPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARI 246

Query: 241 AAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
           A YKVCW D CSDADILAA+DDAIADGVDIISLSVG    R YF+D+IAIGSFHA+E+GI
Sbjct: 247 AVYKVCWRDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGI 306

Query: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ-Y 360
           LTSNSAGN GP   TTASLSPWLLSVAAST+DRKFVT+VQIGN  SFQG SINTFDN   
Sbjct: 307 LTSNSAGNNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQ 366

Query: 361 YPLVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
           YPLV+GR +PN GFD + SS       + KL+KGKI++CEA+F   + F +L G AGVLM
Sbjct: 367 YPLVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDA-KHFVTLGGVAGVLM 426

Query: 421 -TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480
             +   D A SYP+PS++LD ND +AT RYIYS  SP ATIFKST   N  APVVVSFSS
Sbjct: 427 IDTELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSS 486

Query: 481 RGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATY 540
           RGPN  TK++IKPD+SGPGVEILAAWP VA VGGI RNTL+NI+SGTSMSCPHITGIA Y
Sbjct: 487 RGPNNITKEIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAY 546

Query: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
           VKT+NPTWSPAAIKSALMTTA PMNA  N  AEFAYG+GHVNPLKAVRPGLVYDANESDY
Sbjct: 547 VKTFNPTWSPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDY 606

Query: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660
           VKFLCGQGY T  VR IT D SACT  N GRVWDLNYPSFGLSVS S+TFNQYF R LT+
Sbjct: 607 VKFLCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTN 666

Query: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
           VA QASTYRA IS+PQGLTI+VNP VLSFNG+GDRKSFTLTV+G+IK  VVSASLVW DG
Sbjct: 667 VASQASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDG 726

Query: 721 VHSVRSPITITSL 724
           VHSVRSPIT+TSL
Sbjct: 727 VHSVRSPITVTSL 728

BLAST of MELO3C023903.2 vs. TrEMBL
Match: tr|K7NBW1|K7NBW1_SIRGR (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 1.5e-289
Identity = 511/728 (70.19%), Postives = 588/728 (80.77%), Query Frame = 0

Query: 3   SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGS 62
           SSL+FK             S  DS+DDG+ IYIVYMG KLED  SAHL+HRAMLE+VVGS
Sbjct: 7   SSLVFKXXXXXXXXXXXXXSS-DSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGS 66

Query: 63  TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPL 122
           TFAPESV++TYKRSFNGFAVKLTEEEA KIA+ EGVVSVF +E N LHTTRSWDFLG   
Sbjct: 67  TFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQ 126

Query: 123 TVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGAR 182
            VPR  QVESNIVVGV D+GIWPE+PSF+D+GF P P  W+GTC+ S NFRCNRKIIGAR
Sbjct: 127 NVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGAR 186

Query: 183 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 242
           +Y     + PGDV  PRDT+GHGTHTAST AG LVSQA+LYGLG+GTARGGVP ARIA Y
Sbjct: 187 AYR-SSTLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVY 246

Query: 243 KVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 302
           K+CW+DGCSDADILAA+DDAIADGVDIISLSVGG  P+ Y  ++IAIGSFHA++RGILTS
Sbjct: 247 KICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTS 306

Query: 303 NSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQYYPL 362
           NSAGN GP  FT  SLSPWL +VAAS+ DRKFVTQV +GNG ++QGVSINTFD    YPL
Sbjct: 307 NSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMRNQYPL 366

Query: 363 VSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSN 422
           +   + P+ GF+ STS        +P L++GKI++C+++FGP   F S  GAAGVLM SN
Sbjct: 367 IYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGP-TVFASFGGAAGVLMQSN 426

Query: 423 TRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPN 482
           TRD+A SYPLP+SVLDP       RY+ S R+P ATIFKST + + SAPVVVSFSSRGPN
Sbjct: 427 TRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPN 486

Query: 483 RATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIATYVK 542
             T D++KPD + PGVEILAAWP VAP+ G+R  R+ L+NIISGTSMSCPH+T IA ++K
Sbjct: 487 YVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIAVHIK 546

Query: 543 TYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 602
           T+ P+WSPAAIKSALMTTASPMNARFN  AEFAYGSGHVNPLKAV PGLVYDA+ESDYVK
Sbjct: 547 TFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESDYVK 606

Query: 603 FLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTSVA 662
           FLCG+GY T  VR  TGD SACT GN GRVWDLNYPSF LS+S S+T NQ F RTLT+V 
Sbjct: 607 FLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTLTNVV 666

Query: 663 PQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVH 721
             ASTYRA ISAPQGL+ISVNP+VLSFNG+GD+KSFTLTVRG++   +VSASLVWSDG H
Sbjct: 667 SGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVWSDGSH 726

BLAST of MELO3C023903.2 vs. TrEMBL
Match: tr|A0A1S4E3D9|A0A1S4E3D9_CUCME (LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 7.4e-289
Identity = 520/733 (70.94%), Postives = 587/733 (80.08%), Query Frame = 0

Query: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60
           MS SL+F L F +LFFS  LAS LDSDD+G+ IYIVYMG+KL +DPDSA+LHH +     
Sbjct: 7   MSFSLLFNLVFLNLFFSTLLASTLDSDDNGRKIYIVYMGKKLKDDPDSANLHHSSF---- 66

Query: 61  VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
               FAPESV++TY RSFNGFAVKLT+EEA+KIA M+GVVSVF NE+N+LHTTRSWDF+G
Sbjct: 67  ---PFAPESVIYTYNRSFNGFAVKLTKEEADKIAGMKGVVSVFPNEINKLHTTRSWDFMG 126

Query: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
           FP  V R  QV SNIVVGV D+GIWPESPSF+D+GF PPP KWKGTC ++ NF CNRKII
Sbjct: 127 FPQNVRRVKQVGSNIVVGVFDSGIWPESPSFNDKGFDPPPSKWKGTC-SAFNFTCNRKII 186

Query: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
           GAR+YHIGRP+  GDV GPRDT+GHGTH AS A GGLV++A+L GLGLGTARGG+P ARI
Sbjct: 187 GARAYHIGRPLPHGDVEGPRDTDGHGTHCASIAPGGLVNKASLNGLGLGTARGGIPSARI 246

Query: 241 AAYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGI 300
           A YK+CWND     D+LAA+DDAI+DGVDIISLSVGG   R YF D IAIGSFHA++  I
Sbjct: 247 AVYKICWND--DXMDLLAAFDDAISDGVDIISLSVGGNISRKYFRDPIAIGSFHAIQNNI 306

Query: 301 LTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ-Y 360
           LTSNSAGN GPN +T  SLSPWLLSVAASTMDRKFVT+VQIGN +S QGVSINTF     
Sbjct: 307 LTSNSAGNWGPNVYTVTSLSPWLLSVAASTMDRKFVTKVQIGNKRSIQGVSINTFGTTGQ 366

Query: 361 YPLVSGRDIPNPGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
           YPLV+ RD+PN GFD  TS+       N KL+KGKI+ CE+SF P   F S  G AGVLM
Sbjct: 367 YPLVAARDVPNNGFDNLTSTYCFNNSVNLKLVKGKILFCESSFHP-VLFSSFGGVAGVLM 426

Query: 421 TS-NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480
            + N  D A S+PLPSSVL+ +D +    YI S RSP A+I +ST + N  APVVVSFSS
Sbjct: 427 VNVNPLDDALSFPLPSSVLNFHDAITIFDYIRSTRSPNASILRSTAVRNEPAPVVVSFSS 486

Query: 481 RGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATY 540
           RGPN  TK++IKPD+SGPGVEILAAWP VAPVG I RNTL+NIISGTSMSCPHIT IA Y
Sbjct: 487 RGPNNLTKEIIKPDLSGPGVEILAAWPPVAPVGEINRNTLYNIISGTSMSCPHITEIAAY 546

Query: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
           VKT+NPTWSPAAIKSALMTTA PMNA  N +AEFAYGSGHVNP +A+RPGLVYDANE DY
Sbjct: 547 VKTFNPTWSPAAIKSALMTTALPMNATLNLEAEFAYGSGHVNPKRAIRPGLVYDANEIDY 606

Query: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660
           +KFLCGQGY    V  IT    AC   N GRVWDLNYPSFGLSVS SKTF QYF RTLTS
Sbjct: 607 IKFLCGQGYTNGMVEIITSYEDACNSSNIGRVWDLNYPSFGLSVSHSKTFKQYFRRTLTS 666

Query: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
           VA QAS Y+AMISAP+GL I+VNPNVLSFNG+GD+KSF L VRG+IK  +VSASLVWSDG
Sbjct: 667 VASQASKYKAMISAPRGLVITVNPNVLSFNGIGDKKSFKLKVRGTIKESIVSASLVWSDG 726

Query: 721 VHSVRSPITITSL 724
           VHSVRSPITI SL
Sbjct: 727 VHSVRSPITINSL 728

BLAST of MELO3C023903.2 vs. TrEMBL
Match: tr|A0A1S3CFE1|A0A1S3CFE1_CUCME (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500264 PE=4 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 3.7e-264
Identity = 467/735 (63.54%), Postives = 567/735 (77.14%), Query Frame = 0

Query: 2   SSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVG 61
           SSSL+FK   FS  F + LAS LDSD+DG+ IYIVYMG K ED  S   HH  MLE+VVG
Sbjct: 15  SSSLVFKFVVFSSLFCSLLASGLDSDNDGRKIYIVYMGNKPEDSASTPSHHMRMLEEVVG 74

Query: 62  STFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP 121
           S+FAPE++LH+YKRSFNGF VKLTEEEA+KI++ E VVSVF NE   LHTTRSWDF+GF 
Sbjct: 75  SSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGFT 134

Query: 122 LTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGA 181
             VPR  QVESNIVVGVLD+GIWPESPSF D G+ PPP KWKG C+TS NFRCNRKIIGA
Sbjct: 135 QNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGA 194

Query: 182 RSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAA 241
           R+Y   +   P D+  PRD++GHGTHTAST AGGLV+QA+LYGL  GTARGGVP ARIA 
Sbjct: 195 RAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSARIAV 254

Query: 242 YKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 301
           YK+CW+DGC DADILAA+DDAIADGVDIISLSVGG  P++YF D+IAIG+FH+++ GILT
Sbjct: 255 YKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHGILT 314

Query: 302 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYY 361
           SNSAGN GP++FT  + SPW LSVAAS++DRK V++VQ+GN   +QG +INTFD   + Y
Sbjct: 315 SNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKGKQY 374

Query: 362 PLVSGRDIPN--PGFDKSTSS-------NPKLLKGKIVVCEASFGPHEFFKSLDGAAGVL 421
           PL+     PN   GF  S+S        +  L+KGKIV+C++   P  F  SL+GA GV+
Sbjct: 375 PLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFV-SLNGAMGVV 434

Query: 422 MTS-NTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFS 481
           M     +D A SYPLPSS L P D      Y+   + P ATI KS  + + SAP +VSFS
Sbjct: 435 MNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVSFS 494

Query: 482 SRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGIR--RNTLFNIISGTSMSCPHITG 541
           SRGPN  T D++KPD++ PGVEILAAW  +A V  G+R  R TL+NIISGTSMSCPH T 
Sbjct: 495 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 554

Query: 542 IATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDAN 601
            A YVKT++PTWSPAAIKSALMTTA+P+NA+ N Q EFAYG+GH+NPLKAV PGL+YDA 
Sbjct: 555 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYDAY 614

Query: 602 ESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNR 661
           E DYV+FLCGQGY T  VRR++GD S C+  N+GRVWDLNYPSF LS + +++FNQ+F R
Sbjct: 615 ERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFFRR 674

Query: 662 TLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASL 721
           T+T+V  + STYRA ++ AP+GL+I+VNP VLSFN +G +KSFTLT+RGSI+  +VSASL
Sbjct: 675 TVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSASL 734