Lsi04G009170 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi04G009170
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionSubtilisin-like serine endopeptidase family protein
Locationchr04 : 9281448 .. 9285479 (-)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGACAAAAAGTACTACCATGTCTTCTTCTCTGATCTTAAAACTCATCTTACTCAGCCTTTTCTTCAGCCTACTCTCTTCTAACTCTGATTTTGATGATGATGGTAGAAGGGTACAAGACATTATTCAAGTTGGGCCAGTCTACTTTTGATTTAATTTTGATATTTTTTAATTACTTTTTTTTTTTTTTTTTTTGTTATTTTTGTAAAACCTTGTAGATTTATATTGTGTACATGGGGAGCAAGCTAGAGGACCCTGATTCTGCTCATTTGCATCATAGGGCAATGTTGGAAGAAGTTGTTGGCAGGTTAATACTCCTTATTATTTACACTGTTAATTAAAAAACATCAGTCGCCATTCTAATATATTCACTCCAATTTGTTGTTTTGTATGTATTACAAAAAGTACTTTTATGCTTTTTCAGCGATTTTTCTCCAGAATCTTTGATATACACTTACAAGAGAAGTTTTAATGGATTTGCGGTGAAGCTTACCGACGAAGAAGCTCAAAGGATTGTTAGTACGTAATCGTTGTTAAAAAATACTTGCATGCATTCTGAACTATACCATTTGATTTTTTTTTTCAATAATTAATATCATGTACACTTTGTCATAAAACAAACTCTTCTTTCTTTCTTTTCTTTTTTTTTTTTTGAAAAATATTTTTGACTTTTTTCTCTTGGATACATAGTTAAGGTGGGATGGAAAAAAATGGGTGTGTACCCAGCTAGAATGCACCAAAATATATTTTTTTATTCTTTTGTTTCAAATATCATTACAACTGCGTTTGTCATTATTGATTGTTTTATGACTTTTCAATACAACTAATTATAAGTCGTTGTTGACATTTATTATCTATCATTATAAAATTTTCTTTAACAATTTATCATTATTGTCAACAAATTTGTCACACCCCATCCCAAATTAACATATCTAACTTAGAGCATGACATGAAGACATGAAACTCATCATCAATAAAATCGTATAAACCTTAAATGAAGGAAAACCCATCAATGTATGTTTACTGAAAACGGATCACATTTGTATATAAGAAATTTACTAATAAACCCTCCATAATAACCTATTAACAAACTTTAATATGCTCCTTTTTATAACATTTAAAACACACTATAGGAAATCTATAACATAATGTACAAATGTTGATCTAGAGAAGTGGCTATAGGTAAAGTTTTCAGTAAAAAATACGTTAATGGGTTTTCCTTCCTTTAAGTTTTACAGTTTTATTGACGATGAGTTTCATGTTTTCATGTCACACTCTAAGTTAGATAGGTTAATCTGGAGAGGGGCGTGATAAATTACTCAAAAGTTTGATTTTTTTTTATTATTATTTTTATTTTTTATTTATGAAACCTTTATATTGACATTGAGAATCTGTCATAAAAATGTTAATAATGTCAAGAATACCTTATATACGTCATAGGATTACATACAATGACACATATTTATGGTCATTGAGGTTCAACTAATAACAATGTTCCAGTGTCAAATAAATACATCTCATGAAATTTATTAAATGTCAAAGAAATGCTTAATTATAATGATAAAAATTTATTTGTTTGATACATGTCATTAAAATACATTTCCTCCAATTTGGACACAAAATACAAATATGAACTAACAATAGAGTTGGTAACATTAGCTCTTCAAAGTTGTTTATTCTTTTTTTTTTTCAAATATTTTTGAGTTTTTTCAGTCACGTGCGTAGTTGGAGTCTACTGTAAAAAAAATAGGTGTAGTCTAGGATACATCAAGTTATTATTAATTAATCATATTTTTTGTTCATGATTTAGCAGTTCAAAGTTGTTTTATTGTTTCAAAATGTGTATTAAATCACCTGACGAAATGTCTCAGCTAAGGAGGGTGTGGTGTCTGTGTTTCCAAGTGAAAACAACCAACTTCACACGACAAGATCATGGGATTTTCTTGGTTTTCCATCAAATGTTGCTCGCATGAATAAGGTGGAGCAACGTAATCGTTGGAGTTTTCGACAGTGGAATTTGGTCGGACCATCCCAGTTTTAGTGACAAAGGCTACGGTCCTCCTCCAACCAAATGGAAGGGCATTTGCCAATTCTCTGCCAACTTTACTTGCAACAAGTAAACATTTATAAAACACTATATATCCTTATTTAATCTGCGATTTGCACTCAAAATTTTCAATAATTTTATTAATTATCATGAAAATATTTAGAAAAATTATTGGAGCTCGAGCATATCGTAGCAAGAAGACCCTTCCCCCAGGTGACATTAGAAGTCCAATTGATACAAACGGTCATGGAACGCACACTGCGTCGACGGTGGCCGGTGGACTTGTGAACAAAGCAAGTATGAACGGTCTCGGGCTCGCGACAGCGAGAGGAGGGGTCCCCTCTGCACGTATTGCTGTGTACAAAGTATGTTGGTCAGATGAATGTGCCGACGCCGACATGCTTGCAGCATTTGATGATGCCATTGCTGACGGAGTCGATATTATATCCATTTCTGCTGGCGGGAAGTTAACTCAGCCTTATTTTGAACATACCATTTCCATTGGAGCTTTCCATGCATTAAAACGTGGAATATTGACCTCCAGTAAATTGGGTCTCTAGGTTTCAAAACTCACACTTTTAACCTCAGATTTTCACCAAATACTCACTAACTCCAATTAGGGTTAATGTTTATTAATTAATTTAAAAGAATTCCAATCAATTAAGTTGTAGTATTTATCATCACTATTAAAATTAATTTTGTCACACAAGTGCAGCTTGGCAACAAAAATAACTTTCAGGTTGCTTTATTTTTTGCAAATGCATCCATCTTTCTACATCTAATATAATTAATCACACACAAAAGAAAAATTTTAAAATATTTTAAAAAAGAAGTAAAAAAAGTTTTTCATATAGCAAAATAAGATTGAAAAATTGGGTAGGATGCGCAAAAATAGATGTAATGTAGAATGCAAATAACTATTTTTTCGAGAAATATTTACATAATAATTATTTTATATAATTTTTTTAGGTTAAAAATATCGTTTTGGTTCCTATACTTTGAGATTTGTTCAATTTTAGTTTCTATATTTTCAAATGTCTTATATAGGTACCCTCAATAAATCTTAAATTTAGTTTCTATTGCTAGCTTATCGTTGACCTTTTTTTAATAAAAAAAAAATTAGTCCCTATTTTTTGTCTATTGTTGCATTTTTTCCTACAAATTTTGAAAATATATTCATATATTGTATTTTCTTATAAGAAAATTACTACTCTTATTTAACCAATTTTGATAAAAGTTAACTTCGGGAGACTAAATTTACCATTTATTGAAAGTGGGACACTTAAAAATATAAAACTAAAATTGAACAAACTCTAAAGTACAAGGACCAAAATGATATATTAACCATTTTTATATTAAAAAAAATCTAGTAATTTGTTTTAATACTAATAATAAGCTTGTTTTTACATTTTTTTTTTATAGTTAATATGAAAGCTAGTTTAATGAATATAAGACACTATATTATTATCATCTCAAATGTTGATAGTTCAATCTTATATATGTTGAAACACATGCACAATTTGGGTTCTTATTATAAACTTGAAATGCTTTTTTATTCTAATTTTTTATGAAAGTACAGAGGATTTAAGTTAACGCATTCGATGATCTGAAAAATCAATATCTCCTCATTTATGGTGGAGATGTACCAAACAAAGGATCCAATAGCTCCACCTCTAGGTAATCATTTTACTCTCTAATTTATATATGTAAAGATGAATTTGTAGGGTTAAAAAAAACTGAAGTCAATATTATTGAAAATGCAGATTTTGCAAGGAGAACTCAGTGAATCCTAACTTGGTGAAGGGAAAAATCCTTGTTTGTGATGCCCAATTGTCTTCCAAAAAATTTGCTTCCTTGGGTGGCCCAGCCGGCGTCCTTATGCAAGCTTATAGAGATCATGCTGTGTCCTATCCCTTGCCAGCTTCTACTCTCAAGTTAGAAGATGGCAGTAAAATTAAGAGCTACATGACTTCAACTAAGTAA

mRNA sequence

ATGATGACAAAAAGTACTACCATGTCTTCTTCTCTGATCTTAAAACTCATCTTACTCAGCCTTTTCTTCAGCCTACTCTCTTCTAACTCTGATTTTGATGATGATGGTAGAAGGATTTATATTGTGTACATGGGGAGCAAGCTAGAGGACCCTGATTCTGCTCATTTGCATCATAGGGCAATGTTGGAAGAAGTTGTTGGCAGCGATTTTTCTCCAGAATCTTTGATATACACTTACAAGAGAAGTTTTAATGGATTTGCGGTGAAGCTTACCGACGAAGAAGCTCAAAGGATTGTTAGTACTGGAATTTGGTCGGACCATCCCAGTTTTAGTGACAAAGGCTACGGTCCTCCTCCAACCAAATGGAAGGGCATTTGCCAATTCTCTGCCAACTTTACTTGCAACAAAAAAATTATTGGAGCTCGAGCATATCGTAGCAAGAAGACCCTTCCCCCAGGTGACATTAGAAGTCCAATTGATACAAACGGTCATGGAACGCACACTGCGTCGACGGTGGCCGGTGGACTTGTGAACAAAGCAAGTATGAACGGTCTCGGGCTCGCGACAGCGAGAGGAGGGGTCCCCTCTGCACGTATTGCTGTGTACAAAGTATGTTGGTCAGATGAATGTGCCGACGCCGACATGCTTGCAGCATTTGATGATGCCATTGCTGACGGAGTCGATATTATATCCATTTCTGCTGGCGGGAAATTTTGCAAGGAGAACTCAGTGAATCCTAACTTGGTGAAGGGAAAAATCCTTGTTTGTGATGCCCAATTGTCTTCCAAAAAATTTGCTTCCTTGGGTGGCCCAGCCGGCGTCCTTATGCAAGCTTATAGAGATCATGCTGTGTCCTATCCCTTGCCAGCTTCTACTCTCAAGTTAGAAGATGGCAGTAAAATTAAGAGCTACATGACTTCAACTAAGTAA

Coding sequence (CDS)

ATGATGACAAAAAGTACTACCATGTCTTCTTCTCTGATCTTAAAACTCATCTTACTCAGCCTTTTCTTCAGCCTACTCTCTTCTAACTCTGATTTTGATGATGATGGTAGAAGGATTTATATTGTGTACATGGGGAGCAAGCTAGAGGACCCTGATTCTGCTCATTTGCATCATAGGGCAATGTTGGAAGAAGTTGTTGGCAGCGATTTTTCTCCAGAATCTTTGATATACACTTACAAGAGAAGTTTTAATGGATTTGCGGTGAAGCTTACCGACGAAGAAGCTCAAAGGATTGTTAGTACTGGAATTTGGTCGGACCATCCCAGTTTTAGTGACAAAGGCTACGGTCCTCCTCCAACCAAATGGAAGGGCATTTGCCAATTCTCTGCCAACTTTACTTGCAACAAAAAAATTATTGGAGCTCGAGCATATCGTAGCAAGAAGACCCTTCCCCCAGGTGACATTAGAAGTCCAATTGATACAAACGGTCATGGAACGCACACTGCGTCGACGGTGGCCGGTGGACTTGTGAACAAAGCAAGTATGAACGGTCTCGGGCTCGCGACAGCGAGAGGAGGGGTCCCCTCTGCACGTATTGCTGTGTACAAAGTATGTTGGTCAGATGAATGTGCCGACGCCGACATGCTTGCAGCATTTGATGATGCCATTGCTGACGGAGTCGATATTATATCCATTTCTGCTGGCGGGAAATTTTGCAAGGAGAACTCAGTGAATCCTAACTTGGTGAAGGGAAAAATCCTTGTTTGTGATGCCCAATTGTCTTCCAAAAAATTTGCTTCCTTGGGTGGCCCAGCCGGCGTCCTTATGCAAGCTTATAGAGATCATGCTGTGTCCTATCCCTTGCCAGCTTCTACTCTCAAGTTAGAAGATGGCAGTAAAATTAAGAGCTACATGACTTCAACTAAGTAA

Protein sequence

MMTKSTTMSSSLILKLILLSLFFSLLSSNSDFDDDGRRIYIVYMGSKLEDPDSAHLHHRAMLEEVVGSDFSPESLIYTYKRSFNGFAVKLTDEEAQRIVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDAQLSSKKFASLGGPAGVLMQAYRDHAVSYPLPASTLKLEDGSKIKSYMTSTK
Homology
[Close]
BLAST of Lsi04G009170 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.4e-71
Identity = 154/311 (49.52%), Postives = 189/311 (60.77%), Query Frame = 1 

Query: 8   MSSSLILKLILLSLFFS-LLSSNSDFDDDGRRIYIVYMGSKLEDPDSAHLHHRAMLEEVV 67
           MSSSLI KL   SLFFS  L+S  D DDDG+ IYIVYMG KLEDPDSAHLHHRAMLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 68  G----------------SDF-------------SPESLIYTY---------KRSFNGFAV 127
           G                + F             S E ++  +          RS++    
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 128 KLTDEEAQRIVS--------TGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIG 187
            LT     ++ S        TGIW + PSF D+G+ PPP KWKG C+ S NF CN+KIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 188 ARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIA 247
           AR+Y   + + PGD+  P DTNGHGTHTAST AGGLV++A++ GLGL TARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 248 VYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDAQL 272
            YKVCW+D C+D D+LAA+DDAIADGVDIIS+S GG   +   V+  +  G     +  +
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDA-IAIGSFHAVERGI 300

[Close]
BLAST of Lsi04G009170 vs. Swiss-Prot
Match: SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.0e-40
Identity = 96/192 (50.00%), Postives = 125/192 (65.10%), Query Frame = 1 

Query: 62  LEEVVGSDFSPESLIYTYKRSFNGFAVKLTDE---EAQR-----IVSTGIWSDHPSFSDK 121
           +EEVV    +    ++T K S++   + LT +   +A+R     ++ TGI  D  SF D 
Sbjct: 100 MEEVVSVSRNQYRKLHTTK-SWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDH 159

Query: 122 GYGPPPTKWKGICQFSANFT-CNKKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTV 181
           G GPPP KWKG C    NFT CN KIIGA+ ++    +P G++RSPID +GHGTHT+STV
Sbjct: 160 GLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTV 219

Query: 182 AGGLVNKASMNGLGLATARGGVPSARIAVYKVCWS-DECADADMLAAFDDAIADGVDIIS 241
           AG LV  AS+ G+   TARG VPSAR+A+YKVCW+   CAD D+LA F+ AI DGV+IIS
Sbjct: 220 AGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIIS 279

Query: 242 ISAGGKFCKENS 244
           IS GG     +S
Sbjct: 280 ISIGGPIADYSS 290

[Close]
BLAST of Lsi04G009170 vs. Swiss-Prot
Match: SBT4F_ARATH (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.9e-40
Identity = 84/141 (59.57%), Postives = 102/141 (72.34%), Query Frame = 1 

Query: 98  IVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFT-CNKKIIGARAYR-SKKTLPPGDI 157
           ++ TGI  + PSF+DKG GPPP KWKG C    NFT CN K+IGA+ +    + LP G+ 
Sbjct: 142 VLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEG 201

Query: 158 RSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADM 217
            +  D +GHGTHT+ST+AG  V+ AS+ G+   TARGGVPSARIA YKVCW   C D DM
Sbjct: 202 DTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDM 261

Query: 218 LAAFDDAIADGVDIISISAGG 237
           LAAFD+AI+DGVDIISIS GG
Sbjct: 262 LAAFDEAISDGVDIISISIGG 282

[Close]
BLAST of Lsi04G009170 vs. Swiss-Prot
Match: SBT47_ARATH (Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana GN=SBT4.7 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.4e-39
Identity = 81/140 (57.86%), Postives = 96/140 (68.57%), Query Frame = 1 

Query: 99  VSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRSP 158
           + +GIW +  SFSDKG+GPPP KWKG+C    NFTCN K+IGAR Y S+ T         
Sbjct: 138 IDSGIWPESESFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGTR-------- 197

Query: 159 IDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLAA 218
            D  GHGTHTAST AG  V  AS  G+G  TARGGVP++RIA YKVC   +C  A +L+A
Sbjct: 198 -DLQGHGTHTASTAAGNAVADASFFGIGNGTARGGVPASRIAAYKVCSEKDCTAASLLSA 257

Query: 219 FDDAIADGVDIISISAGGKF 239
           FDDAIADGVD+ISIS   +F
Sbjct: 258 FDDAIADGVDLISISLASEF 268

[Close]
BLAST of Lsi04G009170 vs. Swiss-Prot
Match: SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.9e-39
Identity = 79/141 (56.03%), Postives = 97/141 (68.79%), Query Frame = 1 

Query: 98  IVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRS 157
           ++ TGIW +  SFSDKG+GPPP KWKG+C    NFTCN K+IGAR Y S+ T        
Sbjct: 140 VIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGTR------- 199

Query: 158 PIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLA 217
             DT+GHGTHTAST AG  V   S  G+G  T RGGVP++RIA YKVC    C+   +L+
Sbjct: 200 --DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLS 259

Query: 218 AFDDAIADGVDIISISAGGKF 239
           +FDDAIADGVD+I+IS G +F
Sbjct: 260 SFDDAIADGVDLITISIGFQF 271

[Close]
BLAST of Lsi04G009170 vs. TrEMBL
Match: A0A0A0KNZ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187870 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.7e-67
Identity = 152/294 (51.70%), Postives = 177/294 (60.20%), Query Frame = 1 

Query: 8   MSSSLILKLIL-LSLFFSLLSSNSDFDDDGRRIYIVYMGSKL-----EDPDSAHLHHRAM 67
           M SSLI KLI+ L L   LL S S  D D R++YIVYMG KL     +D DSA  HH+ +
Sbjct: 1   MCSSLIFKLIVVLCLISGLLVSCSGSDHDARKVYIVYMGDKLHDTDSDDTDSAPSHHKRI 60

Query: 68  LEEVVGSDFSPESLIYTYKRSFNGFAVKLTDEEAQRIVS--------------------- 127
           LE+   S+F+PE L+++YKRSFNGF  KLT+EEAQ+I +                     
Sbjct: 61  LEKGTSSNFAPEFLLHSYKRSFNGFVAKLTEEEAQKISAMEEVVSLFPNEKKHLHTTRSW 120

Query: 128 --TGIWSDHPSFS-----------------------DKGYGPPPTKWKGICQFSANFTCN 187
              G+  D P                          D GYGP P KWKG CQ SANFTCN
Sbjct: 121 DFIGLTKDAPRVKQVESNLVVGVFDTGIWPENPSFSDVGYGPIPAKWKGTCQTSANFTCN 180

Query: 188 KKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 247
           KKIIGARAYRS    PP DIRSP D++GHGTHTASTV GGLVN+AS  GL   TARGG P
Sbjct: 181 KKIIGARAYRSNNDFPPEDIRSPRDSDGHGTHTASTVVGGLVNEASFYGLAGGTARGGTP 240

Query: 248 SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLV 250
           SA IAVYK+CWSD C   D+LAAFDDAIADGVD+ISIS G        ++P  +
Sbjct: 241 SACIAVYKICWSDGCYSTDILAAFDDAIADGVDMISISLGSPQSSPYFLDPTAI 294

[Close]
BLAST of Lsi04G009170 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 5.7e-59
Identity = 134/239 (56.07%), Postives = 150/239 (62.76%), Query Frame = 1 

Query: 44  MGSKLEDPDSAHLHHRAMLEEVVG------------------------------------ 103
           MG+KLED  S   HH  MLEEVVG                                    
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 104 --SDFSPES--LIYTYKRSFNGF---AVKLTDEEAQRIVS---TGIWSDHPSFSDKGYGP 163
             S F  E   L  T    F GF   A ++   E+  +V    +GIW + PSFSD GYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 164 PPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLV 223
           PP KWKG CQ SANF CN+KIIGARAYRS K  PP DI+SP D++GHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 224 NKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGG 237
           N+AS+ GL L TARGGVPSARIAVYK+CWSD C DAD+LAAFDDAIADGVDIIS+S GG
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG 239

[Close]
BLAST of Lsi04G009170 vs. TrEMBL
Match: A0A0A0KJN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.0e-56
Identity = 151/356 (42.42%), Postives = 186/356 (52.25%), Query Frame = 1 

Query: 11  SLILKLILLSLFFSLLSSNSDFDDDG-RRIYIVYMGSKLE---DPDSAHLHHRAMLEEV- 70
           S + +L+ L L FSLL S S  +DD  R+ YIVYMGS  +    P S+H H R + E V 
Sbjct: 2   SSLSRLLFLCLCFSLLFSTSVSEDDQYRKTYIVYMGSHHQVSSAPLSSHHHMRILQEAVG 61

Query: 71  ------------------------------------VGSDFSPESLIYTYKRSFNGFAVK 130
                                               V S F    L     RS++   + 
Sbjct: 62  STFAPHCLLHSYKRSFNGFVAKLTEIEAKKVSEMEGVISVFPNGELQLHTTRSWDFMGMS 121

Query: 131 LTDEEAQRIVS-----------TGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKI 190
              E+ +R+ S           TGIW + PSF D GYGPPP KWKG C+ SANF+CN KI
Sbjct: 122 ---EQVERVPSVESDIIVGVFDTGIWPESPSFLDHGYGPPPPKWKGSCEVSANFSCNNKI 181

Query: 191 IGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSAR 250
           IGAR+YRS    P  DI+ P D+NGHGTH ASTVAGGLV +ASM GLG+ TARGGVPSAR
Sbjct: 182 IGARSYRSDGRYPIDDIKGPRDSNGHGTHAASTVAGGLVRQASMLGLGMGTARGGVPSAR 241

Query: 251 IAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDA 309
           IA YKVCWSD C+DAD+LAAFDDAIADGVDIIS+S G K  + N     +  G       
Sbjct: 242 IAAYKVCWSDTCSDADVLAAFDDAIADGVDIISMSVGPKRPRPNYFQDPIAIGTFHAMRN 301

[Close]
BLAST of Lsi04G009170 vs. TrEMBL
Match: A0A0A0KRC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 5.9e-56
Identity = 118/219 (53.88%), Postives = 145/219 (66.21%), Query Frame = 1 

Query: 98  IVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRS 157
           +  TGIW + PSF D GYGPPP KWKG C+ SANF+CN KIIGAR+Y S    P GD+  
Sbjct: 137 VFDTGIWPESPSFDDTGYGPPPAKWKGSCEVSANFSCNNKIIGARSYHSSGPHPEGDLEG 196

Query: 158 PIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLA 217
           PID+NGHGTHTASTVAGGLV +A+M GLGL TARGGVPSARIAVYK+CWSD C+DAD+LA
Sbjct: 197 PIDSNGHGTHTASTVAGGLVRQANMLGLGLGTARGGVPSARIAVYKICWSDNCSDADILA 256

Query: 218 AFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDAQLSSKKFASLGGPAGVLMQ 277
           AFDDAIADGVDI+S+S  G   K N  N ++  G        + S   A   GP    + 
Sbjct: 257 AFDDAIADGVDILSVSVAGPGFK-NYFNDSMAIGSFHAMKKGILSSFAAGNTGPGSASVA 316

Query: 278 AYRDHAVSYPLPAST--------LKLEDGSKIKSYMTST 309
            Y   +++  + AST        ++L DG ++K    +T
Sbjct: 317 NYSPWSLT--VAASTTDRVLETVVELGDGRELKGVTINT 352

[Close]
BLAST of Lsi04G009170 vs. TrEMBL
Match: A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 8.5e-55
Identity = 105/139 (75.54%), Postives = 118/139 (84.89%), Query Frame = 1 

Query: 98  IVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRS 157
           ++ +GIW ++PSFSD GYGP P KWKGICQ   NFTCNKKIIGARAYRS    P  DI S
Sbjct: 140 VLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPS 199

Query: 158 PIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLA 217
           P D+NGHGTHTASTVAGGLV++AS+ GL L TARGGVPSARIAVYK+CWSD C+DAD+LA
Sbjct: 200 PRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILA 259

Query: 218 AFDDAIADGVDIISISAGG 237
           AFDDAIADGVDIIS+S GG
Sbjct: 260 AFDDAIADGVDIISLSVGG 278

[Close]
BLAST of Lsi04G009170 vs. TAIR10
Match: AT4G00230.1 (AT4G00230.1 xylem serine peptidase 1)

HSP 1 Score: 168.7 bits (426), Expect = 5.6e-42
Identity = 96/192 (50.00%), Postives = 125/192 (65.10%), Query Frame = 1 

Query: 62  LEEVVGSDFSPESLIYTYKRSFNGFAVKLTDE---EAQR-----IVSTGIWSDHPSFSDK 121
           +EEVV    +    ++T K S++   + LT +   +A+R     ++ TGI  D  SF D 
Sbjct: 100 MEEVVSVSRNQYRKLHTTK-SWDFVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDH 159

Query: 122 GYGPPPTKWKGICQFSANFT-CNKKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTV 181
           G GPPP KWKG C    NFT CN KIIGA+ ++    +P G++RSPID +GHGTHT+STV
Sbjct: 160 GLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTV 219

Query: 182 AGGLVNKASMNGLGLATARGGVPSARIAVYKVCWS-DECADADMLAAFDDAIADGVDIIS 241
           AG LV  AS+ G+   TARG VPSAR+A+YKVCW+   CAD D+LA F+ AI DGV+IIS
Sbjct: 220 AGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIIS 279

Query: 242 ISAGGKFCKENS 244
           IS GG     +S
Sbjct: 280 ISIGGPIADYSS 290

[Close]
BLAST of Lsi04G009170 vs. TAIR10
Match: AT5G03620.1 (AT5G03620.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 167.2 bits (422), Expect = 1.6e-41
Identity = 84/141 (59.57%), Postives = 102/141 (72.34%), Query Frame = 1 

Query: 98  IVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFT-CNKKIIGARAYR-SKKTLPPGDI 157
           ++ TGI  + PSF+DKG GPPP KWKG C    NFT CN K+IGA+ +    + LP G+ 
Sbjct: 142 VLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQSEGLPDGEG 201

Query: 158 RSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADM 217
            +  D +GHGTHT+ST+AG  V+ AS+ G+   TARGGVPSARIA YKVCW   C D DM
Sbjct: 202 DTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWDSGCTDMDM 261

Query: 218 LAAFDDAIADGVDIISISAGG 237
           LAAFD+AI+DGVDIISIS GG
Sbjct: 262 LAAFDEAISDGVDIISISIGG 282

[Close]
BLAST of Lsi04G009170 vs. TAIR10
Match: AT5G58820.1 (AT5G58820.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 164.9 bits (416), Expect = 8.1e-41
Identity = 81/140 (57.86%), Postives = 96/140 (68.57%), Query Frame = 1 

Query: 99  VSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRSP 158
           + +GIW +  SFSDKG+GPPP KWKG+C    NFTCN K+IGAR Y S+ T         
Sbjct: 138 IDSGIWPESESFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGTR-------- 197

Query: 159 IDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLAA 218
            D  GHGTHTAST AG  V  AS  G+G  TARGGVP++RIA YKVC   +C  A +L+A
Sbjct: 198 -DLQGHGTHTASTAAGNAVADASFFGIGNGTARGGVPASRIAAYKVCSEKDCTAASLLSA 257

Query: 219 FDDAIADGVDIISISAGGKF 239
           FDDAIADGVD+ISIS   +F
Sbjct: 258 FDDAIADGVDLISISLASEF 268

[Close]
BLAST of Lsi04G009170 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 164.5 bits (415), Expect = 1.1e-40
Identity = 79/141 (56.03%), Postives = 97/141 (68.79%), Query Frame = 1 

Query: 98  IVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRS 157
           ++ TGIW +  SFSDKG+GPPP KWKG+C    NFTCN K+IGAR Y S+ T        
Sbjct: 140 VIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGTR------- 199

Query: 158 PIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLA 217
             DT+GHGTHTAST AG  V   S  G+G  T RGGVP++RIA YKVC    C+   +L+
Sbjct: 200 --DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEALLS 259

Query: 218 AFDDAIADGVDIISISAGGKF 239
           +FDDAIADGVD+I+IS G +F
Sbjct: 260 SFDDAIADGVDLITISIGFQF 271

[Close]
BLAST of Lsi04G009170 vs. TAIR10
Match: AT5G58830.1 (AT5G58830.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 163.3 bits (412), Expect = 2.4e-40
Identity = 78/140 (55.71%), Postives = 97/140 (69.29%), Query Frame = 1 

Query: 99  VSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIGARAYRSKKTLPPGDIRSP 158
           + +GIW +  SFSDKG+GPPP KWKG+C+   NFTCN K+IGAR Y S+ T         
Sbjct: 133 IDSGIWPESESFSDKGFGPPPKKWKGVCKGGKNFTCNNKLIGARDYTSEGTR-------- 192

Query: 159 IDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIAVYKVCWSDECADADMLAA 218
            D  GHGTHT ST AG  V   S  G+G  TARGGVP++R+A YKVC    C+D ++L+A
Sbjct: 193 -DLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPASRVAAYKVCTITGCSDDNVLSA 252

Query: 219 FDDAIADGVDIISISAGGKF 239
           FDDAIADGVD+IS+S GG +
Sbjct: 253 FDDAIADGVDLISVSLGGDY 263

[Close]
BLAST of Lsi04G009170 vs. NCBI nr
Match: gi|659123557|ref|XP_008461721.1| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis melo])

HSP 1 Score: 271.9 bits (694), Expect = 1.3e-69
Identity = 155/311 (49.84%), Postives = 190/311 (61.09%), Query Frame = 1 

Query: 8   MSSSLILKLILLSLFFS-LLSSNSDFDDDGRRIYIVYMGSKLEDPDSAHLHHRAMLEEVV 67
           MSSSLI KL   SLFFS  L+S  D DDDG+ IYIVYMG KLEDPDSAHLHHRAMLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 68  G----------------SDF-------------SPESLIYTY---------KRSFNGFAV 127
           G                + F             S E ++  +          RS++    
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 128 KLTDEEAQRIVS--------TGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIG 187
            LT     ++ S        TGIW + PSF D+G+ PPP KWKG C+ S NF CN+KIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 188 ARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIA 247
           AR+Y   + + PGD+  P DTNGHGTHTAST AGGLV++A++ GLGL TARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 248 VYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDAQL 272
            YKVCW+D C+DAD+LAA+DDAIADGVDIIS+S GG   +   V+  +  G     +  +
Sbjct: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDA-IAIGSFHAVERGI 300

[Close]
BLAST of Lsi04G009170 vs. NCBI nr
Match: gi|778701133|ref|XP_004149947.2| (PREDICTED: cucumisin-like [Cucumis sativus])

HSP 1 Score: 271.6 bits (693), Expect = 1.7e-69
Identity = 158/283 (55.83%), Postives = 183/283 (64.66%), Query Frame = 1 

Query: 1   MMTKSTTMSSSLILKLILLS-LFFSLLSSNSDFDDDGRRIYIVYMGSKLEDPDSAHLHHR 60
           M+++ +  SSSLI K +  S LF SLL+S+ D D+DGR+IYIVYMG+KLED  S   HH 
Sbjct: 3   MISRRSISSSSLIFKFVFFSSLFSSLLASSLDSDNDGRKIYIVYMGNKLEDSASTPSHHM 62

Query: 61  AMLEEVVG--------------------------------------SDFSPES--LIYTY 120
            MLEEVVG                                      S F  E   L  T 
Sbjct: 63  RMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTR 122

Query: 121 KRSFNGF---AVKLTDEEAQRIVS---TGIWSDHPSFSDKGYGPPPTKWKGICQFSANFT 180
              F GF   A ++   E+  +V    +GIW + PSFSD GYGPPP KWKG CQ SANF 
Sbjct: 123 SWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQTSANFH 182

Query: 181 CNKKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGG 237
           CN+KIIGARAYRS K  PP DI+SP D++GHGTHTASTVAGGLVN+AS+ GL L TARGG
Sbjct: 183 CNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGG 242

[Close]
BLAST of Lsi04G009170 vs. NCBI nr
Match: gi|71153243|sp|Q39547.1|CUCM1_CUCME (RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor [Cucumis melo])

HSP 1 Score: 270.4 bits (690), Expect = 3.9e-69
Identity = 154/311 (49.52%), Postives = 189/311 (60.77%), Query Frame = 1 

Query: 8   MSSSLILKLILLSLFFS-LLSSNSDFDDDGRRIYIVYMGSKLEDPDSAHLHHRAMLEEVV 67
           MSSSLI KL   SLFFS  L+S  D DDDG+ IYIVYMG KLEDPDSAHLHHRAMLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 68  G----------------SDF-------------SPESLIYTY---------KRSFNGFAV 127
           G                + F             S E ++  +          RS++    
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 128 KLTDEEAQRIVS--------TGIWSDHPSFSDKGYGPPPTKWKGICQFSANFTCNKKIIG 187
            LT     ++ S        TGIW + PSF D+G+ PPP KWKG C+ S NF CN+KIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 188 ARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVPSARIA 247
           AR+Y   + + PGD+  P DTNGHGTHTAST AGGLV++A++ GLGL TARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 248 VYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLVKGKILVCDAQL 272
            YKVCW+D C+D D+LAA+DDAIADGVDIIS+S GG   +   V+  +  G     +  +
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDA-IAIGSFHAVERGI 300

[Close]
BLAST of Lsi04G009170 vs. NCBI nr
Match: gi|659123579|ref|XP_008461729.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 265.8 bits (678), Expect = 9.6e-68
Identity = 154/283 (54.42%), Postives = 179/283 (63.25%), Query Frame = 1 

Query: 1   MMTKSTTMSSSLILKLILLS-LFFSLLSSNSDFDDDGRRIYIVYMGSKLEDPDSAHLHHR 60
           M+++ +  SSSL+ K ++ S LF SLL+S  D D+DGR+IYIVYMG+K ED  S   HH 
Sbjct: 20  MISRRSMSSSSLVFKFVVFSSLFCSLLASGLDSDNDGRKIYIVYMGNKPEDSASTPSHHM 79

Query: 61  AMLEEVVG--------------------------------------SDFSPES--LIYTY 120
            MLEEVVG                                      S F  E   L  T 
Sbjct: 80  RMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTR 139

Query: 121 KRSFNGFA-----VKLTDEE-AQRIVSTGIWSDHPSFSDKGYGPPPTKWKGICQFSANFT 180
              F GF      VK  +      ++ +GIW + PSFSD GYGPPP KWKG CQ SANF 
Sbjct: 140 SWDFMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFR 199

Query: 181 CNKKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGG 237
           CN+KIIGARAYRS K  PP DI+SP D++GHGTHTASTVAGGLVN+AS+ GL   TARGG
Sbjct: 200 CNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGG 259

[Close]
BLAST of Lsi04G009170 vs. NCBI nr
Match: gi|778701136|ref|XP_011654970.1| (PREDICTED: cucumisin-like [Cucumis sativus])

HSP 1 Score: 262.7 bits (670), Expect = 8.1e-67
Identity = 152/294 (51.70%), Postives = 177/294 (60.20%), Query Frame = 1 

Query: 8   MSSSLILKLIL-LSLFFSLLSSNSDFDDDGRRIYIVYMGSKL-----EDPDSAHLHHRAM 67
           M SSLI KLI+ L L   LL S S  D D R++YIVYMG KL     +D DSA  HH+ +
Sbjct: 1   MCSSLIFKLIVVLCLISGLLVSCSGSDHDARKVYIVYMGDKLHDTDSDDTDSAPSHHKRI 60

Query: 68  LEEVVGSDFSPESLIYTYKRSFNGFAVKLTDEEAQRIVS--------------------- 127
           LE+   S+F+PE L+++YKRSFNGF  KLT+EEAQ+I +                     
Sbjct: 61  LEKGTSSNFAPEFLLHSYKRSFNGFVAKLTEEEAQKISAMEEVVSLFPNEKKHLHTTRSW 120

Query: 128 --TGIWSDHPSFS-----------------------DKGYGPPPTKWKGICQFSANFTCN 187
              G+  D P                          D GYGP P KWKG CQ SANFTCN
Sbjct: 121 DFIGLTKDAPRVKQVESNLVVGVFDTGIWPENPSFSDVGYGPIPAKWKGTCQTSANFTCN 180

Query: 188 KKIIGARAYRSKKTLPPGDIRSPIDTNGHGTHTASTVAGGLVNKASMNGLGLATARGGVP 247
           KKIIGARAYRS    PP DIRSP D++GHGTHTASTV GGLVN+AS  GL   TARGG P
Sbjct: 181 KKIIGARAYRSNNDFPPEDIRSPRDSDGHGTHTASTVVGGLVNEASFYGLAGGTARGGTP 240

Query: 248 SARIAVYKVCWSDECADADMLAAFDDAIADGVDIISISAGGKFCKENSVNPNLV 250
           SA IAVYK+CWSD C   D+LAAFDDAIADGVD+ISIS G        ++P  +
Sbjct: 241 SACIAVYKICWSDGCYSTDILAAFDDAIADGVDMISISLGSPQSSPYFLDPTAI 294

The following BLAST results are available for this feature:
BLAST of Lsi04G009170 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CUCM1_CUCME2.4e-7149.52Cucumisin OS=Cucumis melo PE=1 SV=1[more]
SBT4E_ARATH1.0e-4050.00Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1[more]
SBT4F_ARATH2.9e-4059.57Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1[more]
SBT47_ARATH1.4e-3957.86Subtilisin-like protease SBT4.7 OS=Arabidopsis thaliana GN=SBT4.7 PE=3 SV=1[more]
SBT4C_ARATH1.9e-3956.03Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1[more]
BLAST of Lsi04G009170 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KNZ5_CUCSA5.7e-6751.70Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187870 PE=4 SV=1[more]
A0A0A0KLR4_CUCSA5.7e-5956.07Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1[more]
A0A0A0KJN5_CUCSA2.0e-5642.42Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1[more]
A0A0A0KRC4_CUCSA5.9e-5653.88Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1[more]
A0A0A0KLY4_CUCSA8.5e-5575.54Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1[more]
BLAST of Lsi04G009170 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G00230.15.6e-4250.00 xylem serine peptidase 1[more]
AT5G03620.11.6e-4159.57 Subtilisin-like serine endopeptidase family protein[more]
AT5G58820.18.1e-4157.86 Subtilisin-like serine endopeptidase family protein[more]
AT5G59090.11.1e-4056.03 subtilase 4.12[more]
AT5G58830.12.4e-4055.71 Subtilisin-like serine endopeptidase family protein[more]
BLAST of Lsi04G009170 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659123557|ref|XP_008461721.1|1.3e-6949.84PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis melo][more]
gi|778701133|ref|XP_004149947.2|1.7e-6955.83PREDICTED: cucumisin-like [Cucumis sativus][more]
gi|71153243|sp|Q39547.1|CUCM1_CUCME3.9e-6949.52RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor [Cucumis me... [more]
gi|659123579|ref|XP_008461729.1|9.6e-6854.42PREDICTED: cucumisin-like isoform X1 [Cucumis melo][more]
gi|778701136|ref|XP_011654970.1|8.1e-6751.70PREDICTED: cucumisin-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: INTERPRO
TermDefinition
IPR015500Peptidase_S8_subtilisin-rel
IPR010259S8pro/Inhibitor_I9
IPR000209Peptidase_S8/S53_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
cellular_component GO:0005576 extracellular region
molecular_function GO:0004252 serine-type endopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G009170.1Lsi04G009170.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 98..239
score: 5.7
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 98..244
score: 3.1
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 98..127
score: 3.27E-27coord: 157..238
score: 3.27
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 40..99
score: 8.0
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 7..308
score: 1.4E
NoneNo IPR availablePANTHERPTHR10795:SF348SUBFAMILY NOT NAMEDcoord: 7..308
score: 1.4E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi04G009170Cla003979Watermelon (97103) v1lsiwmB307
Lsi04G009170ClCG07G003700Watermelon (Charleston Gray)lsiwcgB295
Lsi04G009170MELO3C023903.2Melon (DHL92) v3.6.1lsimedB297
Lsi04G009170Bhi07G000589Wax gourdlsiwgoB385
Lsi04G009170Bhi06G000880Wax gourdlsiwgoB353
Lsi04G009170Bhi07G000583Wax gourdlsiwgoB390
Lsi04G009170Cp4.1LG01g10970Cucurbita pepo (Zucchini)cpelsiB358
Lsi04G009170Cp4.1LG01g05180Cucurbita pepo (Zucchini)cpelsiB359
Lsi04G009170Carg00244Silver-seed gourdcarlsiB608
Lsi04G009170Carg18344Silver-seed gourdcarlsiB080
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi04G009170Watermelon (97103) v2lsiwmbB262
Lsi04G009170Watermelon (97103) v2lsiwmbB274
Lsi04G009170Watermelon (97103) v2lsiwmbB296
Lsi04G009170Wax gourdlsiwgoB366
Lsi04G009170Bottle gourd (USVL1VR-Ls)lsilsiB077
Lsi04G009170Bottle gourd (USVL1VR-Ls)lsilsiB104
Lsi04G009170Bottle gourd (USVL1VR-Ls)lsilsiB127
Lsi04G009170Bottle gourd (USVL1VR-Ls)lsilsiB129
Lsi04G009170Cucumber (Gy14) v1cgylsiB066
Lsi04G009170Cucumber (Gy14) v1cgylsiB217
Lsi04G009170Cucumber (Gy14) v1cgylsiB276
Lsi04G009170Cucumber (Gy14) v1cgylsiB299
Lsi04G009170Cucurbita maxima (Rimu)cmalsiB244
Lsi04G009170Cucurbita maxima (Rimu)cmalsiB616
Lsi04G009170Cucurbita maxima (Rimu)cmalsiB676
Lsi04G009170Cucurbita maxima (Rimu)cmalsiB677
Lsi04G009170Cucurbita maxima (Rimu)cmalsiB686
Lsi04G009170Cucurbita maxima (Rimu)cmalsiB756
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB267
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB232
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB603
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB669
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB670
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB679
Lsi04G009170Cucurbita moschata (Rifu)cmolsiB751
Lsi04G009170Cucurbita pepo (Zucchini)cpelsiB152
Lsi04G009170Wild cucumber (PI 183967)cpilsiB052
Lsi04G009170Wild cucumber (PI 183967)cpilsiB195
Lsi04G009170Wild cucumber (PI 183967)cpilsiB366
Lsi04G009170Wild cucumber (PI 183967)cpilsiB459
Lsi04G009170Cucumber (Chinese Long) v2culsiB052
Lsi04G009170Cucumber (Chinese Long) v2culsiB196
Lsi04G009170Cucumber (Chinese Long) v2culsiB363
Lsi04G009170Cucumber (Chinese Long) v2culsiB452
Lsi04G009170Melon (DHL92) v3.5.1lsimeB281
Lsi04G009170Melon (DHL92) v3.5.1lsimeB300
Lsi04G009170Melon (DHL92) v3.5.1lsimeB308
Lsi04G009170Melon (DHL92) v3.5.1lsimeB332
Lsi04G009170Watermelon (Charleston Gray)lsiwcgB256
Lsi04G009170Watermelon (Charleston Gray)lsiwcgB280
Lsi04G009170Watermelon (Charleston Gray)lsiwcgB282
Lsi04G009170Watermelon (97103) v1lsiwmB287
Lsi04G009170Watermelon (97103) v1lsiwmB301
Lsi04G009170Watermelon (97103) v1lsiwmB330
Lsi04G009170Watermelon (97103) v1lsiwmB338
Lsi04G009170Cucumber (Gy14) v2cgyblsiB331
Lsi04G009170Cucumber (Gy14) v2cgyblsiB418
Lsi04G009170Melon (DHL92) v3.6.1lsimedB313
Lsi04G009170Silver-seed gourdcarlsiB239
Lsi04G009170Silver-seed gourdcarlsiB405
Lsi04G009170Silver-seed gourdcarlsiB916
Lsi04G009170Cucumber (Chinese Long) v3cuclsiB058
Lsi04G009170Cucumber (Chinese Long) v3cuclsiB214
Lsi04G009170Cucumber (Chinese Long) v3cuclsiB387
Lsi04G009170Cucumber (Chinese Long) v3cuclsiB481