CmaCh04G001840 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh04G001840 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 5.4e-269
Identity = 476/735 (64.76%), Postives = 566/735 (77.01%), Query Frame = 1

Query: 41  SSSLIFKLVILNLVCC-LLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIG 100
           SSSLIFKL   +L     LAS  DS +DG+ +YIVY+G K ED  S   HH  MLE+ +G
Sbjct: 2   SSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVG 61

Query: 101 STFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFT 160
           STFAPE++LH+YKRSFNGF V+LTEEEA+KI++ +GVVSVF NE    HTTRSWDF+GF 
Sbjct: 62  STFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFP 121

Query: 161 TNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGA 220
             VPR  QVES+IVVGVLDTGIWPESPSF D G+ PPP KWKGTC+ S NFRCN+KIIGA
Sbjct: 122 LTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGA 181

Query: 221 RAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAV 280
           R+Y       P D   PRD+ GHGTHTAST AGGLVSQA+L+GL LGTARGGVP ARIA 
Sbjct: 182 RSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAA 241

Query: 281 YKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILT 340
           YKVCW+DGC D DILAA+DDAIADGVDIISLSVGG  P+ YF D+IAIG+FH+++ GILT
Sbjct: 242 YKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILT 301

Query: 341 SNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQF 400
           SNSAGN GP+F T  + SPW LSVAAS++DRK V++VQ+GN   FQG +INTFD Q   +
Sbjct: 302 SNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQY--Y 361

Query: 401 PLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTF-ASFSSAVGVV 460
           PL+   + PN   GF  +TSRFC+  SV+ NL+KGKI+VC++   P  F  S   A GV+
Sbjct: 362 PLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVL 421

Query: 461 MNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFS 520
           M  +  +D A SYPLPSS L          Y+ S   P ATIFKS  + + SAP+VVSFS
Sbjct: 422 MT-SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFS 481

Query: 521 SRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATA 580
           SRGPN  T D++KPD++ PGVEILAAWP  APV GGIR  R+TL+NIISGTSMSCPH T 
Sbjct: 482 SRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GGIR--RNTLFNIISGTSMSCPHITG 541

Query: 581 AAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDAN 640
            A YVKT++PTWSPAAIKSALMTTA P+N   NPQAEFAYG+GH++PLKA  PGLVYDAN
Sbjct: 542 IATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDAN 601

Query: 641 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTR 700
           E+DYV FLCGQGY+T  VRR+TGD S CT+ N+GRVWDLNYPSF LS +PS++ NQ+F R
Sbjct: 602 ESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNR 661

Query: 701 TLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASV 760
           TLT+V  +ASTY + I  AP+GLTI+V+P VLSFNG+GD+KSFTLT+ G+I   +VSAS+
Sbjct: 662 TLTSVAPQASTYRAMI-SAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASL 721

Query: 761 VWSDGSHNVRSPITI 774
           VWSDG H VRSPITI
Sbjct: 722 VWSDGVHYVRSPITI 727

BLAST of CmaCh04G001840 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 3.6e-188
Identity = 353/734 (48.09%), Postives = 473/734 (64.44%), Query Frame = 1

Query: 51  LNLVCCLLASSFD-SGNDGRK---VYIVYLGNKREDTASTPSHHMRMLEETIGSTFAPEA 110
           L L+C     + D S ND R+   VYIVY+G   E   S PSHH+ +L++ +G+  A   
Sbjct: 8   LYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHL 67

Query: 111 LLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVPRVK 170
           L+ SYKRSFNGF   L++ E+QK+     VVSVFP++     TTRSWDF+GF     R  
Sbjct: 68  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 127

Query: 171 QVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDN 230
             ESD++VGV+D+GIWPES SF D G+GPPP KWKG+C+    F CN K+IGAR Y   N
Sbjct: 128 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY---N 187

Query: 231 NFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSD 290
            F      S RD EGHGTHTAST AG  V  AS +GLA GTARGGVPSARIA YKVC+ +
Sbjct: 188 KFA----DSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-N 247

Query: 291 GCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGND 350
            C D DILAAFDDAIADGVD+IS+S+  +      N S+AIG+FH+M  GI+T+ SAGN+
Sbjct: 248 RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 307

Query: 351 GPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGN 410
           GPD  +V N SPW ++VAAS  DR+ + +V LGN     G ++NTF+L   +FP++Y   
Sbjct: 308 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVY--- 367

Query: 411 APNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKD 470
             N+S   + A + +CS   VD  LVKGKI++CD  L     A  + A+GV++ +  + D
Sbjct: 368 GQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPD 427

Query: 471 NARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVT 530
           +A   P P+S LG     +IK+Y+ S   P A I ++  + D  AP V SFSSRGP+ V 
Sbjct: 428 SAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVI 487

Query: 531 LDILKPDLTAPGVEILAAWPPDAPVSGGI--RDSRSTLYNIISGTSMSCPHATAAAVYVK 590
            ++LKPD++APG+EILAA+ P A  S  +   D RS  Y+++SGTSM+CPH    A YVK
Sbjct: 488 QNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVK 547

Query: 591 TFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETDYVN 650
           +FHP WSP+AIKSA+MTTA P+N+  NP+ EFAYG+G I+P KA++PGLVY+    DY+ 
Sbjct: 548 SFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLK 607

Query: 651 FLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLTNVD 710
            LC +G+ +  +   +G    C+      V DLNYP+     +  +  N  F RT+TNV 
Sbjct: 608 MLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVG 667

Query: 711 TEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIK--TIVSASVVWSD 770
              STY + ++     L I+++P +L F  + +KKSF +TI G  +K  + VS+SVVWSD
Sbjct: 668 FPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSD 727

Query: 771 GSHNVRSPITIYIV 777
           GSH+VRSPI  Y +
Sbjct: 728 GSHSVRSPIVAYSI 727

BLAST of CmaCh04G001840 vs. Swiss-Prot
Match: SBT4D_ARATH (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 1.6e-180
Identity = 348/750 (46.40%), Postives = 480/750 (64.00%), Query Frame = 1

Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTP-SHHMRM 94
           MA   ASSSL+  L++L L      SS  +  D ++VYIVY+G+       TP S HM +
Sbjct: 1   MATLAASSSLLSCLLVLFL------SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNI 60

Query: 95  LEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSW 154
           L+E  G +     L+ SYKRSFNGF  RLTE E ++++   GVVSVFPN+K    TT SW
Sbjct: 61  LQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSW 120

Query: 155 DFMGFTTNVP--RVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFR 214
           DFMG    +   R   VESD ++GV+D+GI PES SFSD G+GPPP KWKG C    NF 
Sbjct: 121 DFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT 180

Query: 215 CNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGG 274
           CN K+IGAR Y S+           RD +GHGTHTAST AG  V  AS FG+  GT RGG
Sbjct: 181 CNNKLIGARDYTSEGT---------RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGG 240

Query: 275 VPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFH 334
           VP++R+A YKVC   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH
Sbjct: 241 VPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFH 300

Query: 335 SMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINT 394
           +M  G+LT NSAGN GP  ++V   +PW L+VAAS+ +R  V+KV LGN     G ++N 
Sbjct: 301 AMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNA 360

Query: 395 FDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASF 454
           ++++ K +PL+Y  +A   S      ++  C  + VD++ VKGKILVC          S 
Sbjct: 361 YEMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV 420

Query: 455 SSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSA 514
             AVG++       D A  +PLP++ L T    ++ +Y+ S + P A + K+ A+ + ++
Sbjct: 421 -GAVGLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTS 480

Query: 515 PIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSM 574
           P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P    S    D+R   Y+++SGTSM
Sbjct: 481 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSM 540

Query: 575 SCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN---VGLNPQAEFAYGAGHIDPLKA 634
           SCPH    A YVKTF+P WSP+ I+SA+MTTA P+N    G+    EFAYG+GH+DP+ A
Sbjct: 541 SCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGI-ASTEFAYGSGHVDPIAA 600

Query: 635 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 694
           +NPGLVY+ +++D++ FLCG  Y++ +++ ++G+   C+ A      +LNYPS +   + 
Sbjct: 601 SNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSG 660

Query: 695 S-ESINQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLTID 754
           S  +    F RTLTNV T  STYTSK++ G    L + + P VLSF  + +K+SFT+T+ 
Sbjct: 661 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 720

Query: 755 GTIIKTIV--SASVVWSDGSHNVRSPITIY 775
           G+ + + V  SA+++WSDG+HNVRSPI +Y
Sbjct: 721 GSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of CmaCh04G001840 vs. Swiss-Prot
Match: SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.7e-177
Identity = 336/735 (45.71%), Postives = 471/735 (64.08%), Query Frame = 1

Query: 53  LVCCLLASSFDSGNDG-RKVYIVYLGNKREDTASTP-SHHMRMLEETIGSTFAPEALLHS 112
           LV  L + S D  + G ++VYIVYLG+       TP S HM +L+E  G +     L+ S
Sbjct: 15  LVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRS 74

Query: 113 YKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVP--RVKQV 172
           YK+SFNGF  RLTE E ++++  + VVSVFP+ K    TT SW+FMG    +   R + +
Sbjct: 75  YKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSI 134

Query: 173 ESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNF 232
           ESD ++GV+D+GI+PES SFSD G+GPPP KWKGTC    NF CN K+IGAR Y + +  
Sbjct: 135 ESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKA 194

Query: 233 PPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGC 292
                ++ RD  GHGTHTAS  AG  V+ ++ +GL  GTARGGVP+ARIAVYKVC ++GC
Sbjct: 195 N----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGC 254

Query: 293 EDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGP 352
           +   +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP
Sbjct: 255 DGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGP 314

Query: 353 DFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAP 412
              TV + +PW  SVAAS  +R  ++KV LG+  I  G ++NT+D+    +PL+Y  +A 
Sbjct: 315 KISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAA 374

Query: 413 NISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNA 472
             +     A  R C    +D  LVKGKI++CDS           +   +V N    +   
Sbjct: 375 LSTCSVDKA--RLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFI 434

Query: 473 RSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLD 532
           RS+P+  S+L      ++ +YM S   P AT+ KS  +++  AP+V SFSSRGP+ +  D
Sbjct: 435 RSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSD 494

Query: 533 ILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHP 592
           ILKPD+TAPGVEILAA+ PD+  +    D+R   Y+++SGTSM+CPH    A YVKTFHP
Sbjct: 495 ILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHP 554

Query: 593 TWSPAAIKSALMTTAIPLNVGLN--PQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFL 652
            WSP+ I+SA+MTTA P+N   +     EFAYG+GH+DP+ A NPGLVY+  + D++NFL
Sbjct: 555 QWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFL 614

Query: 653 CGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESINQFFTRTLTNVDT 712
           CG  Y++  +R ++GD S CT   S  +  +LNYP+ +   + ++  N  F RT+TNV  
Sbjct: 615 CGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGM 674

Query: 713 EASTYTSKILGAP-EGLTITVDPPVLSFNGIGDKKSFTLTIDGTII--KTIVSASVVWSD 772
           + STY +K++  P   L+I V P VLS   + +K+SF +T+    I  K  VSA+++WSD
Sbjct: 675 QKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSD 734

Query: 773 GSHNVRSPITIYIVN 778
           G+HNVRSPI +Y ++
Sbjct: 735 GTHNVRSPIIVYAMS 741

BLAST of CmaCh04G001840 vs. Swiss-Prot
Match: SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 3.7e-177
Identity = 345/754 (45.76%), Postives = 478/754 (63.40%), Query Frame = 1

Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGN--KREDTASTPSHHMR 94
           MA   AS+ L   L++L     LL+S     ++  +VYIVY+G+   R D   T S HM 
Sbjct: 1   MANLAASTCLYSWLLVL-----LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPT-SDHMS 60

Query: 95  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 154
           +L++  G +     L+ SYKRSFNGF  RLTE E   I+  +GVVSVFPN+    HTT S
Sbjct: 61  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120

Query: 155 WDFMGFTT--NVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNF 214
           WDFMG     N  R   +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C    NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180

Query: 215 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 274
            CN K+IGAR Y S+           RD+ GHGTHTAST AG  V   S FG+  GT RG
Sbjct: 181 TCNNKLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 240

Query: 275 GVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 334
           GVP++RIA YKVC   GC    +L++FDDAIADGVD+I++S+G   P  + +D IAIGAF
Sbjct: 241 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 300

Query: 335 HSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 394
           H+M  GILT +SAGN GP   TV + +PW  +VAAS+ +R  ++KV LGN     G ++N
Sbjct: 301 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 360

Query: 395 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFAS 454
            FD++ K++PL+Y  +A   S      T+  C+   ++++ VKGKILVC     PS +  
Sbjct: 361 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 420

Query: 455 FSSAVGVVMNDAGVK-DNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDT 514
             S   + + D   + D A ++ LP+S L      ++ +Y+ S + P A + K+  + + 
Sbjct: 421 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 480

Query: 515 SAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGT 574
           ++P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P+   S    D+R   Y++ SGT
Sbjct: 481 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 540

Query: 575 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV---GLNPQAEFAYGAGHIDPL 634
           SM+CPH    A YVKTF+P WSP+ I+SA+MTTA P+     G+    EFAYGAGH+DP+
Sbjct: 541 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGI-ASTEFAYGAGHVDPM 600

Query: 635 KATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALST 694
            A NPGLVY+ ++ D++ FLCG  Y++  ++ ++GD   C+  N     +LNYPS +   
Sbjct: 601 AALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKL 660

Query: 695 TPSES-INQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLT 754
           + ++S  +  F RTLTNV T  STY SK++ G    L+I V P VL F  + +K+SF++T
Sbjct: 661 SGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVT 720

Query: 755 IDGTIIKTIV--SASVVWSDGSHNVRSPITIYIV 777
           + G+ + + V  SA+++WSDG+HNVRSPI +YI+
Sbjct: 721 VTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731

BLAST of CmaCh04G001840 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 580/704 (82.39%), Postives = 638/704 (90.62%), Query Frame = 1

Query: 76  LGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDG 135
           +GNK ED+ASTPSHHMRMLEE +GS+FAPEALLHSYKRSFNGFVV+LTEEEAQKISAK+ 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 136 VVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGP 195
           VVSVFPNEKKH HTTRSWDFMGFT   PRVKQVES+IVVGVLD+GIWPESPSFSDVGYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 196 PPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLV 255
           PP KWKG CQ S NF CN+KIIGARAYRSD  FPPED KSPRDS+GHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 256 SQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGN 315
           +QASL+GLALGTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 316 EPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSK 375
           +PK+YFNDSIAIGAFHSMK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVS+
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 376 VQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGK 435
           VQLGN N FQG+TINTFDL+ KQ PLIYAG+APNIS GFTG++SRFCSRNSVDRNLVKGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 436 ILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNF 495
           I++CDS+LSP+TF S + AVGVVMND GVKDNARSYPLPSSYL  V G+NIKTYM    F
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 496 PTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGI 555
           PTATI KSNAVNDTSAP +VSFSSRGPNP T DILKPDLTAPGVEILAAW P A VS G+
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 556 RDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAE 615
           RDSR+TLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PLN  LN Q E
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 616 FAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVW 675
           FAYGAGHI+PL+A +PGL+YDA E+DYV FLCGQGY+TAMVRRL+GD SVCT ANSGRVW
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 676 DLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGI 735
           DLNYPSFALS+T S+S NQFF RT+TNV ++ STY +K++G P GL+ITV+PPVLSFN I
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660

Query: 736 GDKKSFTLTIDGTIIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           G KKSFTLTI G+I ++IVSAS+VWSDG HNVRSPIT+++V  A
Sbjct: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 704

BLAST of CmaCh04G001840 vs. TrEMBL
Match: A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 587/740 (79.32%), Postives = 652/740 (88.11%), Query Frame = 1

Query: 41  SSSLIFKL---VILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEET 100
           SSSLIFKL   ++L LV  LLAS FDS ND RK+YIVY+GNK +DTASTPSHHMRML E 
Sbjct: 2   SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLREV 61

Query: 101 IGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMG 160
            GS FAPE+LLHSYKRSFNGFVV+LTEEEA +ISAK+GVVSVFP+ KKH HTTRSWDF+G
Sbjct: 62  TGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIG 121

Query: 161 FTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKII 220
           FT +VPRV QVESDIVVGVLD+GIWPE+PSFSD GYGP PAKWKG CQ  TNF CNKKII
Sbjct: 122 FTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKII 181

Query: 221 GARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARI 280
           GARAYRSDN FP ED  SPRDS GHGTHTASTVAGGLVSQASL+GLALGTARGGVPSARI
Sbjct: 182 GARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARI 241

Query: 281 AVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGI 340
           AVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+E ++YFNDSIAIGAFHSMK+GI
Sbjct: 242 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHGI 301

Query: 341 LTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQK 400
           LTSNSAGNDGPD+ T+RNFSPWSLSVAAS+ DRKLVS+V++GN N++QG+TINTFD   K
Sbjct: 302 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLGK 361

Query: 401 QFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGV 460
           Q+PLIYAG+APN+ GGFTG+ SRFCS  SVD NLV GKIL+CDSIL+PS F  FS AVGV
Sbjct: 362 QYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVGV 421

Query: 461 VMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSF 520
           VMND GVK  + SYPLPSSYL TV G+ IKTYM SN  PTATIFKS+AVND+SAP +VSF
Sbjct: 422 VMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVSF 481

Query: 521 SSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHAT 580
           SSRGPNP TLDILKPDLTAPGVEILAAW P APVS G+ DSR+TLYNIISGTSMSCPH T
Sbjct: 482 SSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHVT 541

Query: 581 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDA 640
           AAAVYVKTFHPTWSPAAIKSALMTTA PL   +N +AEFAYGAG I+PLKA +PGLVYDA
Sbjct: 542 AAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYDA 601

Query: 641 NETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFT 700
           NE DYV FLCGQGY++ MV+ L+ D ++C +AN GRVWDLNYPSFALS+TPS+SINQFFT
Sbjct: 602 NEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFFT 661

Query: 701 RTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTI-IKTIVSA 760
           RTLT+VD+ ASTYTS ILGAP+GLTITV+P VLSF+GIG+KK+FTLTI GTI   TIVSA
Sbjct: 662 RTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVSA 721

Query: 761 SVVWSDGSHNVRSPITIYIV 777
           S+VWSD SH+VRSPITIY+V
Sbjct: 722 SLVWSDSSHDVRSPITIYVV 741

BLAST of CmaCh04G001840 vs. TrEMBL
Match: A0A0A0KNZ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187870 PE=4 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 541/745 (72.62%), Postives = 625/745 (83.89%), Query Frame = 1

Query: 42  SSLIFKL-VILNLVCCLLASSFDSGNDGRKVYIVYLGNK-----REDTASTPSHHMRMLE 101
           SSLIFKL V+L L+  LL S   S +D RKVYIVY+G+K      +DT S PSHH R+LE
Sbjct: 3   SSLIFKLIVVLCLISGLLVSCSGSDHDARKVYIVYMGDKLHDTDSDDTDSAPSHHKRILE 62

Query: 102 ETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDF 161
           +   S FAPE LLHSYKRSFNGFV +LTEEEAQKISA + VVS+FPNEKKH HTTRSWDF
Sbjct: 63  KGTSSNFAPEFLLHSYKRSFNGFVAKLTEEEAQKISAMEEVVSLFPNEKKHLHTTRSWDF 122

Query: 162 MGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKK 221
           +G T + PRVKQVES++VVGV DTGIWPE+PSFSDVGYGP PAKWKGTCQ S NF CNKK
Sbjct: 123 IGLTKDAPRVKQVESNLVVGVFDTGIWPENPSFSDVGYGPIPAKWKGTCQTSANFTCNKK 182

Query: 222 IIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSA 281
           IIGARAYRS+N+FPPED +SPRDS+GHGTHTASTV GGLV++AS +GLA GTARGG PSA
Sbjct: 183 IIGARAYRSNNDFPPEDIRSPRDSDGHGTHTASTVVGGLVNEASFYGLAGGTARGGTPSA 242

Query: 282 RIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKN 341
            IAVYK+CWSDGC   DILAAFDDAIADGVD+IS+S+G  +   YF D  AIGAFH+MKN
Sbjct: 243 CIAVYKICWSDGCYSTDILAAFDDAIADGVDMISISLGSPQSSPYFLDPTAIGAFHAMKN 302

Query: 342 GILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQ 401
           GILTS SAGN+GP++ +V N +PW+LSV AS+IDRKL SKV+LGN NI+QGFTINTFDL+
Sbjct: 303 GILTSTSAGNEGPNYFSVSNVAPWALSVGASTIDRKLASKVELGNRNIYQGFTINTFDLE 362

Query: 402 QKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAV 461
            KQ+PLIYA +APNI+GGFTG+ SRFCS NSV+ NLVKGK+LVCDS+L PS F +FS AV
Sbjct: 363 GKQYPLIYARDAPNIAGGFTGSMSRFCSANSVNANLVKGKVLVCDSVLPPSRFVNFSDAV 422

Query: 462 GVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVV 521
           GV+MND   KD++ SYPLPSSYL T  GNN+KTYM SN  PTATI+KSNA+NDTSAP+VV
Sbjct: 423 GVIMNDGRTKDSSGSYPLPSSYLTTADGNNVKTYMSSNGAPTATIYKSNAINDTSAPLVV 482

Query: 522 SFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPH 581
           SFSSRGPNP T DILKPDLTAPGV+ILAAW P APVS G+ DSR+TLYNIISGTSMSCPH
Sbjct: 483 SFSSRGPNPQTFDILKPDLTAPGVQILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPH 542

Query: 582 ATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVY 641
            TAAAVYVKTFHPTWSPAAI+SALMTTA PL+  LN QAEFAYGAG IDP+KA +PGLVY
Sbjct: 543 VTAAAVYVKTFHPTWSPAAIQSALMTTATPLSAVLNMQAEFAYGAGQIDPVKAIDPGLVY 602

Query: 642 DANETDYVNFLCGQGYSTAMVRRLTGD-GSVCTAANSGRVWDLNYPSFALSTTPSESINQ 701
           DA E+DYV FLCGQGY+T+MV+R + D  +VC + N GRVWDLNYPSFALS++PS   NQ
Sbjct: 603 DAGESDYVKFLCGQGYTTSMVQRFSNDKNTVCNSTNMGRVWDLNYPSFALSSSPSRPFNQ 662

Query: 702 FFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIV 761
           +FTRTLTNV ++ASTYTS + G P+GLTITV+P  LSFN  G K++FTLTI GT+  +I 
Sbjct: 663 YFTRTLTNVGSKASTYTSTVRGTPQGLTITVNPTSLSFNSTGQKRNFTLTIRGTVSSSIA 722

Query: 762 SASVVWSDGSHNVRSPITIYIVNKA 780
           SAS++WSDGSHNVRSPIT++ V  A
Sbjct: 723 SASLIWSDGSHNVRSPITVFKVASA 747

BLAST of CmaCh04G001840 vs. TrEMBL
Match: K7NBW1_SIRGR (Cucumisin OS=Siraitia grosvenorii PE=2 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 1.9e-300
Identity = 519/735 (70.61%), Postives = 613/735 (83.40%), Query Frame = 1

Query: 42  SSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETIGST 101
           SSL+FKL+ L+L C LL SS DS +DGRK+YIVY+G+K EDTAS   +H  MLEE +GST
Sbjct: 7   SSLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGST 66

Query: 102 FAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTN 161
           FAPE+++++YKRSFNGF V+LTEEEA KI+AK+GVVSVFP+EK H HTTRSWDF+G + N
Sbjct: 67  FAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQN 126

Query: 162 VPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARA 221
           VPRVKQVES+IVVGV D+GIWPE+PSF+D G+GP PA W+GTCQASTNFRCN+KIIGARA
Sbjct: 127 VPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGARA 186

Query: 222 YRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYK 281
           YRS +  PP D +SPRD++GHGTHTASTVAG LVSQASL+GL +GTARGGVP ARIAVYK
Sbjct: 187 YRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVYK 246

Query: 282 VCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSN 341
           +CWSDGC DADILAAFDDAIADGVDIISLSVGG  P+ Y  +SIAIG+FH+MK GILTSN
Sbjct: 247 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTSN 306

Query: 342 SAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPL 401
           SAGN+GP   TV + SPW  +VAASS DRK V++V LGN N +QG +INTFD+ + Q+PL
Sbjct: 307 SAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDM-RNQYPL 366

Query: 402 IYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMND 461
           IYAGNAP+I  GF  +TSR+C  +SVD NLV+GKIL+CDS   P+ FASF  A GV+M  
Sbjct: 367 IYAGNAPSI--GFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLM-Q 426

Query: 462 AGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRG 521
           +  +D+A SYPLP+S L    GNNIK YM S   PTATIFKS  V DTSAP+VVSFSSRG
Sbjct: 427 SNTRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRG 486

Query: 522 PNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAV 581
           PN VT DILKPD TAPGVEILAAWPP AP+S G+RDSRS LYNIISGTSMSCPH TA AV
Sbjct: 487 PNYVTHDILKPDSTAPGVEILAAWPPVAPIS-GVRDSRSALYNIISGTSMSCPHVTAIAV 546

Query: 582 YVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDANETD 641
           ++KTF+P+WSPAAIKSALMTTA P+N   N  AEFAYG+GH++PLKA +PGLVYDA+E+D
Sbjct: 547 HIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESD 606

Query: 642 YVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLT 701
           YV FLCG+GY+TAMVR  TGD S CT+ N GRVWDLNYPSFALS + S++ NQ F RTLT
Sbjct: 607 YVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTLT 666

Query: 702 NVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASVVWS 761
           NV + ASTY + I  AP+GL+I+V+P VLSFNGIGD+KSFTLT+ GT+ + IVSAS+VWS
Sbjct: 667 NVVSGASTYRASI-SAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVWS 726

Query: 762 DGSHNVRSPITIYIV 777
           DGSHNVRSPIT+Y++
Sbjct: 727 DGSHNVRSPITVYVL 734

BLAST of CmaCh04G001840 vs. TrEMBL
Match: D7TLL6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g02480 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 6.7e-266
Identity = 458/701 (65.34%), Postives = 547/701 (78.03%), Query Frame = 1

Query: 76  LGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDG 135
           +G+K     S  + H  ML++  GS  A ++LL+SYKRSFNGFVV+LTEEE +++   DG
Sbjct: 1   MGDKPSGDISAVTAHTNMLQQVFGSNIASDSLLYSYKRSFNGFVVKLTEEEMKELEGMDG 60

Query: 136 VVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGP 195
           VVS+FPNEKK  HTTRSWDF+GF   V R   VESD+++ VLDTGIWPES SF D G+GP
Sbjct: 61  VVSIFPNEKKKLHTTRSWDFIGFPQQVNRTS-VESDVIIAVLDTGIWPESDSFKDKGFGP 120

Query: 196 PPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLV 255
           PP+KWKG CQ  +NF CN KIIGAR YRS   F PED ++PRDSEGHGTHTAST AGGLV
Sbjct: 121 PPSKWKGICQGLSNFTCNNKIIGARYYRSYGEFSPEDLQTPRDSEGHGTHTASTAAGGLV 180

Query: 256 SQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGN 315
           S ASL G  LGTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+
Sbjct: 181 SMASLLGFGLGTARGGVPSARIAVYKICWSDGCADADILAAFDDAIADGVDIISLSVGGS 240

Query: 316 EPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSK 375
            PK YF DSIAIGAFH+MKNGILTS SAGNDGP+F ++ NFSPWSLSVAAS+IDRK  +K
Sbjct: 241 TPKNYFADSIAIGAFHAMKNGILTSTSAGNDGPNFASITNFSPWSLSVAASTIDRKFFTK 300

Query: 376 VQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGK 435
           VQLG++ +++G +INTF+     +P IY G+APNI+GGF+  TSRFC+RNS+D NLVKGK
Sbjct: 301 VQLGDSKVYEGISINTFE-PNGMYPFIYGGDAPNITGGFSANTSRFCTRNSLDPNLVKGK 360

Query: 436 ILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNF 495
           I++CD I S  T A  + AVG VM D G KD+A  +PLP+SYLG   G++I  Y+ S + 
Sbjct: 361 IVLCD-IFSNGTGAFLAGAVGTVMADRGAKDSAWPFPLPASYLGAQDGSSIAYYVTSTSN 420

Query: 496 PTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGI 555
           PTA+I KS  VNDT AP +VSFSSRGPNP TLDILKPDL APGV ILAAWPP +P+SG  
Sbjct: 421 PTASILKSTEVNDTLAPFIVSFSSRGPNPATLDILKPDLAAPGVHILAAWPPISPISGVQ 480

Query: 556 RDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAE 615
            D+R+ LY + SGTSM+CPHAT AA Y+K+FHPTWSPAAIKSALMTTA+P++   NP AE
Sbjct: 481 GDTRAVLYTMQSGTSMACPHATGAAAYIKSFHPTWSPAAIKSALMTTALPMSAEKNPDAE 540

Query: 616 FAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVW 675
           FAYGAG IDPLK+ NPGLVYDA++ DYV FLCGQGY+T  ++ +TGD SVC+ A +G VW
Sbjct: 541 FAYGAGQIDPLKSVNPGLVYDADKIDYVKFLCGQGYTTQTLQLVTGDNSVCSEATNGTVW 600

Query: 676 DLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGI 735
           DLNYPSFALS++  ESI   FTRT+TNV +  STY + + GAP GL I V P +LSF  +
Sbjct: 601 DLNYPSFALSSSTFESITGVFTRTVTNVGSPVSTYKATVTGAPIGLQIQVVPDILSFTSL 660

Query: 736 GDKKSFTLTIDGTIIKTIVSASVVWSDGSHNVRSPITIYIV 777
           G K SF L ++G +   IVSAS+VW DG H VRSPI + I+
Sbjct: 661 GQKLSFVLKVEGKVGDNIVSASLVWDDGVHQVRSPIVVSIL 698

BLAST of CmaCh04G001840 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 646.0 bits (1665), Expect = 3.0e-185
Identity = 340/705 (48.23%), Postives = 457/705 (64.82%), Query Frame = 1

Query: 76  LGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDG 135
           +G   E   S PSHH+ +L++ +G+  A   L+ SYKRSFNGF   L++ E+QK+     
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 136 VVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGP 195
           VVSVFP++     TTRSWDF+GF     R    ESD++VGV+D+GIWPES SF D G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 196 PPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLV 255
           PP KWKG+C+    F CN K+IGAR Y   N F      S RD EGHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY---NKFAD----SARDEEGHGTHTASTAAGNAV 180

Query: 256 SQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGN 315
             AS +GLA GTARGGVPSARIA YKVC++  C D DILAAFDDAIADGVD+IS+S+  +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISAD 240

Query: 316 EPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSK 375
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  +V N SPW ++VAAS  DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 376 VQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGK 435
           V LGN     G ++NTF+L   +FP++Y     N+S   + A + +CS   VD  LVKGK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 436 ILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNF 495
           I++CD  L     A  + A+GV++ +  + D+A   P P+S LG     +IK+Y+ S   
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 496 PTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGI 555
           P A I ++  + D  AP V SFSSRGP+ V  ++LKPD++APG+EILAA+ P A  S  +
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 556 --RDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQ 615
              D RS  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTA P+N+  NP+
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 616 AEFAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGR 675
            EFAYG+G I+P KA++PGLVY+    DY+  LC +G+ +  +   +G    C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 676 VWDLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFN 735
           V DLNYP+     +  +  N  F RT+TNV    STY + ++     L I+++P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 736 GIGDKKSFTLTIDGTIIK--TIVSASVVWSDGSHNVRSPITIYIV 777
            + +KKSF +TI G  +K  + VS+SVVWSDGSH+VRSPI  Y +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of CmaCh04G001840 vs. TAIR10
Match: AT5G59120.1 (AT5G59120.1 subtilase 4.13)

HSP 1 Score: 634.4 bits (1635), Expect = 9.1e-182
Identity = 348/750 (46.40%), Postives = 480/750 (64.00%), Query Frame = 1

Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTP-SHHMRM 94
           MA   ASSSL+  L++L L      SS  +  D ++VYIVY+G+       TP S HM +
Sbjct: 1   MATLAASSSLLSCLLVLFL------SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNI 60

Query: 95  LEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSW 154
           L+E  G +     L+ SYKRSFNGF  RLTE E ++++   GVVSVFPN+K    TT SW
Sbjct: 61  LQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSW 120

Query: 155 DFMGFTTNVP--RVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFR 214
           DFMG    +   R   VESD ++GV+D+GI PES SFSD G+GPPP KWKG C    NF 
Sbjct: 121 DFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT 180

Query: 215 CNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGG 274
           CN K+IGAR Y S+           RD +GHGTHTAST AG  V  AS FG+  GT RGG
Sbjct: 181 CNNKLIGARDYTSEGT---------RDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGG 240

Query: 275 VPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFH 334
           VP++R+A YKVC   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH
Sbjct: 241 VPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFH 300

Query: 335 SMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINT 394
           +M  G+LT NSAGN GP  ++V   +PW L+VAAS+ +R  V+KV LGN     G ++N 
Sbjct: 301 AMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNA 360

Query: 395 FDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASF 454
           ++++ K +PL+Y  +A   S      ++  C  + VD++ VKGKILVC          S 
Sbjct: 361 YEMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV 420

Query: 455 SSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSA 514
             AVG++       D A  +PLP++ L T    ++ +Y+ S + P A + K+ A+ + ++
Sbjct: 421 -GAVGLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTS 480

Query: 515 PIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSM 574
           P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P    S    D+R   Y+++SGTSM
Sbjct: 481 PVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSM 540

Query: 575 SCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN---VGLNPQAEFAYGAGHIDPLKA 634
           SCPH    A YVKTF+P WSP+ I+SA+MTTA P+N    G+    EFAYG+GH+DP+ A
Sbjct: 541 SCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGI-ASTEFAYGSGHVDPIAA 600

Query: 635 TNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTP 694
           +NPGLVY+ +++D++ FLCG  Y++ +++ ++G+   C+ A      +LNYPS +   + 
Sbjct: 601 SNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSG 660

Query: 695 S-ESINQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLTID 754
           S  +    F RTLTNV T  STYTSK++ G    L + + P VLSF  + +K+SFT+T+ 
Sbjct: 661 SGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVT 720

Query: 755 GTIIKTIV--SASVVWSDGSHNVRSPITIY 775
           G+ + + V  SA+++WSDG+HNVRSPI +Y
Sbjct: 721 GSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of CmaCh04G001840 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 624.4 bits (1609), Expect = 9.5e-179
Identity = 336/735 (45.71%), Postives = 471/735 (64.08%), Query Frame = 1

Query: 53  LVCCLLASSFDSGNDG-RKVYIVYLGNKREDTASTP-SHHMRMLEETIGSTFAPEALLHS 112
           LV  L + S D  + G ++VYIVYLG+       TP S HM +L+E  G +     L+ S
Sbjct: 15  LVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIENRLVRS 74

Query: 113 YKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTNVP--RVKQV 172
           YK+SFNGF  RLTE E ++++  + VVSVFP+ K    TT SW+FMG    +   R + +
Sbjct: 75  YKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTKRTRSI 134

Query: 173 ESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYRSDNNF 232
           ESD ++GV+D+GI+PES SFSD G+GPPP KWKGTC    NF CN K+IGAR Y + +  
Sbjct: 135 ESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYTAKSKA 194

Query: 233 PPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVCWSDGC 292
                ++ RD  GHGTHTAS  AG  V+ ++ +GL  GTARGGVP+ARIAVYKVC ++GC
Sbjct: 195 N----QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVCDNEGC 254

Query: 293 EDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGP 352
           +   +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M  G+LT N+AGN+GP
Sbjct: 255 DGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGP 314

Query: 353 DFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAP 412
              TV + +PW  SVAAS  +R  ++KV LG+  I  G ++NT+D+    +PL+Y  +A 
Sbjct: 315 KISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAA 374

Query: 413 NISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMNDAGVKDNA 472
             +     A  R C    +D  LVKGKI++CDS           +   +V N    +   
Sbjct: 375 LSTCSVDKA--RLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDRAFI 434

Query: 473 RSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLD 532
           RS+P+  S+L      ++ +YM S   P AT+ KS  +++  AP+V SFSSRGP+ +  D
Sbjct: 435 RSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSD 494

Query: 533 ILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAVYVKTFHP 592
           ILKPD+TAPGVEILAA+ PD+  +    D+R   Y+++SGTSM+CPH    A YVKTFHP
Sbjct: 495 ILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHP 554

Query: 593 TWSPAAIKSALMTTAIPLNVGLN--PQAEFAYGAGHIDPLKATNPGLVYDANETDYVNFL 652
            WSP+ I+SA+MTTA P+N   +     EFAYG+GH+DP+ A NPGLVY+  + D++NFL
Sbjct: 555 QWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFL 614

Query: 653 CGQGYSTAMVRRLTGDGSVCTAANSGRV-WDLNYPSFALSTTPSESINQFFTRTLTNVDT 712
           CG  Y++  +R ++GD S CT   S  +  +LNYP+ +   + ++  N  F RT+TNV  
Sbjct: 615 CGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGM 674

Query: 713 EASTYTSKILGAP-EGLTITVDPPVLSFNGIGDKKSFTLTIDGTII--KTIVSASVVWSD 772
           + STY +K++  P   L+I V P VLS   + +K+SF +T+    I  K  VSA+++WSD
Sbjct: 675 QKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSD 734

Query: 773 GSHNVRSPITIYIVN 778
           G+HNVRSPI +Y ++
Sbjct: 735 GTHNVRSPIIVYAMS 741

BLAST of CmaCh04G001840 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 623.2 bits (1606), Expect = 2.1e-178
Identity = 345/754 (45.76%), Postives = 478/754 (63.40%), Query Frame = 1

Query: 35  MAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGN--KREDTASTPSHHMR 94
           MA   AS+ L   L++L     LL+S     ++  +VYIVY+G+   R D   T S HM 
Sbjct: 1   MANLAASTCLYSWLLVL-----LLSSVSAIIDEDTQVYIVYMGSLSSRADYIPT-SDHMS 60

Query: 95  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 154
           +L++  G +     L+ SYKRSFNGF  RLTE E   I+  +GVVSVFPN+    HTT S
Sbjct: 61  ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120

Query: 155 WDFMGFTT--NVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNF 214
           WDFMG     N  R   +ESD ++GV+DTGIWPES SFSD G+GPPP KWKG C    NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180

Query: 215 RCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARG 274
            CN K+IGAR Y S+           RD+ GHGTHTAST AG  V   S FG+  GT RG
Sbjct: 181 TCNNKLIGARDYTSEGT---------RDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRG 240

Query: 275 GVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAF 334
           GVP++RIA YKVC   GC    +L++FDDAIADGVD+I++S+G   P  + +D IAIGAF
Sbjct: 241 GVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAF 300

Query: 335 HSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTIN 394
           H+M  GILT +SAGN GP   TV + +PW  +VAAS+ +R  ++KV LGN     G ++N
Sbjct: 301 HAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVN 360

Query: 395 TFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFAS 454
            FD++ K++PL+Y  +A   S      T+  C+   ++++ VKGKILVC     PS +  
Sbjct: 361 AFDMKGKKYPLVYGKSA--ASSACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKI 420

Query: 455 FSSAVGVVMNDAGVK-DNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDT 514
             S   + + D   + D A ++ LP+S L      ++ +Y+ S + P A + K+  + + 
Sbjct: 421 AKSVGAIAIIDKSPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNR 480

Query: 515 SAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGT 574
           ++P++ SFSSRGPN + +DILKPD+TAPGVEILAA+ P+   S    D+R   Y++ SGT
Sbjct: 481 TSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGT 540

Query: 575 SMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNV---GLNPQAEFAYGAGHIDPL 634
           SM+CPH    A YVKTF+P WSP+ I+SA+MTTA P+     G+    EFAYGAGH+DP+
Sbjct: 541 SMACPHVAGVAAYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGI-ASTEFAYGAGHVDPM 600

Query: 635 KATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALST 694
            A NPGLVY+ ++ D++ FLCG  Y++  ++ ++GD   C+  N     +LNYPS +   
Sbjct: 601 AALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKL 660

Query: 695 TPSES-INQFFTRTLTNVDTEASTYTSKIL-GAPEGLTITVDPPVLSFNGIGDKKSFTLT 754
           + ++S  +  F RTLTNV T  STY SK++ G    L+I V P VL F  + +K+SF++T
Sbjct: 661 SGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVT 720

Query: 755 IDGTIIKTIV--SASVVWSDGSHNVRSPITIYIV 777
           + G+ + + V  SA+++WSDG+HNVRSPI +YI+
Sbjct: 721 VTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIM 731

BLAST of CmaCh04G001840 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 618.2 bits (1593), Expect = 6.8e-177
Identity = 338/739 (45.74%), Postives = 465/739 (62.92%), Query Frame = 1

Query: 50  ILNLVCCLLASSFDSG---NDGRKVYIVYLGNKREDTASTP-SHHMRMLEETIGSTFAPE 109
           +L+ +  LL  SF S    +  ++VYIVY+G         P SHH  +L++  G +   +
Sbjct: 9   LLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQD 68

Query: 110 ALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGFTTN--VP 169
            L+ +YKRSFNGF  RLTE E + +++ D VVSVFP++  +  TT SW+FMG        
Sbjct: 69  RLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTK 128

Query: 170 RVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIGARAYR 229
           R   +ESD ++GV+D+GI+PES SFS  G+GPPP KWKG C+  TNF CN K+IGAR Y 
Sbjct: 129 RNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARYYT 188

Query: 230 SDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIAVYKVC 289
                 PE   S RD+ GHG+HTAS  AG  V   S +GL  GT RGGVP+ARIAVYKVC
Sbjct: 189 PKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVC 248

Query: 290 WSD--GCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGILTSN 349
                 C    ILAAFDDAIAD VDII++S+G +    +  D++AIGAFH+M  GILT N
Sbjct: 249 DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVN 308

Query: 350 SAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQFPL 409
            AGN+GP+  T+ + +PW  +VAAS+++R  ++KV LGN     G ++N+FDL  K++PL
Sbjct: 309 GAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPL 368

Query: 410 IYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVVMND 469
           +Y  +A   S     +++ FCS   +D   VKGKI++CD+  +P    +  +   +V N 
Sbjct: 369 VYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIVRNP 428

Query: 470 AGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFSSRG 529
              +D A  +  P S L     N + +Y+ S   P A + KS  + +  AP+V S+SSRG
Sbjct: 429 --YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRG 488

Query: 530 PNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATAAAV 589
           PNP+  DILKPD+TAPG EILAA+ P  P S    D+R   Y +ISGTSMSCPH    A 
Sbjct: 489 PNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKYTVISGTSMSCPHVAGVAA 548

Query: 590 YVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQ---AEFAYGAGHIDPLKATNPGLVYDAN 649
           Y+KTFHP WSP+ I+SA+MTTA P+N   +P    AEFAYGAGH+DP+ A +PGLVY+AN
Sbjct: 549 YIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEAN 608

Query: 650 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVW-DLNYPSFALSTTPSESINQFFT 709
           ++D++ FLCG  Y+   +R ++GD S CT   +  +  +LNYPS +   + ++     F 
Sbjct: 609 KSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKVTFR 668

Query: 710 RTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIK--TIVS 769
           RT+TNV    +TY +K++G+   L + V P VLS   + +KKSFT+T+ G   K   +VS
Sbjct: 669 RTVTNVGRPNATYKAKVVGSK--LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVS 728

Query: 770 ASVVWSDGSHNVRSPITIY 775
           A ++WSDG H VRSPI +Y
Sbjct: 729 AQLIWSDGVHFVRSPIVVY 735

BLAST of CmaCh04G001840 vs. NCBI nr
Match: gi|659123579|ref|XP_008461729.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 614/756 (81.22%), Postives = 680/756 (89.95%), Query Frame = 1

Query: 27  IFMFPVMTMAGNNA--SSSLIFKLVILN-LVCCLLASSFDSGNDGRKVYIVYLGNKREDT 86
           +FMFP+M M    +  SSSL+FK V+ + L C LLAS  DS NDGRK+YIVY+GNK ED+
Sbjct: 12  LFMFPLMRMISRRSMSSSSLVFKFVVFSSLFCSLLASGLDSDNDGRKIYIVYMGNKPEDS 71

Query: 87  ASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNE 146
           ASTPSHHMRMLEE +GS+FAPEALLHSYKRSFNGFVV+LTEEEAQKISAK+ VVSVFPNE
Sbjct: 72  ASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNE 131

Query: 147 KKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGT 206
           KKH HTTRSWDFMGFT NVPRVKQVES+IVVGVLD+GIWPESPSFSDVGYGPPPAKWKG 
Sbjct: 132 KKHLHTTRSWDFMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGA 191

Query: 207 CQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGL 266
           CQ S NFRCN+KIIGARAYRSD  FPPED KSPRDS+GHGTHTASTVAGGLV+QASL+GL
Sbjct: 192 CQTSANFRCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGL 251

Query: 267 ALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFND 326
           A GTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGGN PK+YFND
Sbjct: 252 ASGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFND 311

Query: 327 SIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNI 386
           SIAIGAFHSMK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVS+VQLGN NI
Sbjct: 312 SIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNI 371

Query: 387 FQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIL 446
           +QG+TINTFDL+ KQ+PLIYAG+APNISGGFTG++SRFCSRNSVDRNLVKGKI++CDS+L
Sbjct: 372 YQGYTINTFDLKGKQYPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVL 431

Query: 447 SPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKS 506
           SP+TF S + A+GVVMND GVKDNARSYPLPSSYL  V GNNIKTYM  N FPTATI KS
Sbjct: 432 SPATFVSLNGAMGVVMNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKS 491

Query: 507 NAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLY 566
           NAVNDTSAP +VSFSSRGPNP T DILKPDLTAPGVEILAAW P A VS G+RDSR+TLY
Sbjct: 492 NAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLY 551

Query: 567 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHI 626
           NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PLN  LN Q EFAYGAGHI
Sbjct: 552 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHI 611

Query: 627 DPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFA 686
           +PLKA +PGL+YDA E DYV FLCGQGY+TAMVRRL+GD S C+ ANSGRVWDLNYPSFA
Sbjct: 612 NPLKAVHPGLLYDAYERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFA 671

Query: 687 LSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTL 746
           LS+T ++S NQFF RT+TNV ++ STY +K++GAP GL+ITV+PPVLSFN IG KKSFTL
Sbjct: 672 LSSTSAQSFNQFFRRTVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTL 731

Query: 747 TIDGTIIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           TI G+I ++IVSAS++WSDG HNVRSPIT+++V  A
Sbjct: 732 TIRGSIRQSIVSASLMWSDGYHNVRSPITVFVVGTA 767

BLAST of CmaCh04G001840 vs. NCBI nr
Match: gi|778701133|ref|XP_004149947.2| (PREDICTED: cucumisin-like [Cucumis sativus])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 605/741 (81.65%), Postives = 666/741 (89.88%), Query Frame = 1

Query: 40  ASSSLIFKLVILN-LVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEETI 99
           +SSSLIFK V  + L   LLASS DS NDGRK+YIVY+GNK ED+ASTPSHHMRMLEE +
Sbjct: 10  SSSSLIFKFVFFSSLFSSLLASSLDSDNDGRKIYIVYMGNKLEDSASTPSHHMRMLEEVV 69

Query: 100 GSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMGF 159
           GS+FAPEALLHSYKRSFNGFVV+LTEEEAQKISAK+ VVSVFPNEKKH HTTRSWDFMGF
Sbjct: 70  GSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFMGF 129

Query: 160 TTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKIIG 219
           T   PRVKQVES+IVVGVLD+GIWPESPSFSDVGYGPPP KWKG CQ S NF CN+KIIG
Sbjct: 130 TQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQTSANFHCNRKIIG 189

Query: 220 ARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARIA 279
           ARAYRSD  FPPED KSPRDS+GHGTHTASTVAGGLV+QASL+GLALGTARGGVPSARIA
Sbjct: 190 ARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIA 249

Query: 280 VYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGIL 339
           VYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG++PK+YFNDSIAIGAFHSMK+GIL
Sbjct: 250 VYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHGIL 309

Query: 340 TSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQKQ 399
           TSNSAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVS+VQLGN N FQG+TINTFDL+ KQ
Sbjct: 310 TSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQGYTINTFDLKGKQ 369

Query: 400 FPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGVV 459
            PLIYAG+APNIS GFTG++SRFCSRNSVDRNLVKGKI++CDS+LSP+TF S + AVGVV
Sbjct: 370 HPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAVGVV 429

Query: 460 MNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSFS 519
           MND GVKDNARSYPLPSSYL  V G+NIKTYM    FPTATI KSNAVNDTSAP +VSFS
Sbjct: 430 MNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKSNAVNDTSAPWIVSFS 489

Query: 520 SRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHATA 579
           SRGPNP T DILKPDLTAPGVEILAAW P A VS G+RDSR+TLYNIISGTSMSCPHATA
Sbjct: 490 SRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHATA 549

Query: 580 AAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDAN 639
           AAVYVKTFHPTWSPAAIKSALMTTA PLN  LN Q EFAYGAGHI+PL+A +PGL+YDA 
Sbjct: 550 AAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLRAVHPGLLYDAY 609

Query: 640 ETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTR 699
           E+DYV FLCGQGY+TAMVRRL+GD SVCT ANSGRVWDLNYPSFALS+T S+S NQFF R
Sbjct: 610 ESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTSSQSFNQFFRR 669

Query: 700 TLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTIIKTIVSASV 759
           T+TNV ++ STY +K++G P GL+ITV+PPVLSFN IG KKSFTLTI G+I ++IVSAS+
Sbjct: 670 TVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSISQSIVSASL 729

Query: 760 VWSDGSHNVRSPITIYIVNKA 780
           VWSDG HNVRSPIT+++V  A
Sbjct: 730 VWSDGHHNVRSPITVFVVGTA 750

BLAST of CmaCh04G001840 vs. NCBI nr
Match: gi|700195413|gb|KGN50590.1| (hypothetical protein Csa_5G187880 [Cucumis sativus])

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 580/704 (82.39%), Postives = 638/704 (90.62%), Query Frame = 1

Query: 76  LGNKREDTASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDG 135
           +GNK ED+ASTPSHHMRMLEE +GS+FAPEALLHSYKRSFNGFVV+LTEEEAQKISAK+ 
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 136 VVSVFPNEKKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGP 195
           VVSVFPNEKKH HTTRSWDFMGFT   PRVKQVES+IVVGVLD+GIWPESPSFSDVGYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 196 PPAKWKGTCQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLV 255
           PP KWKG CQ S NF CN+KIIGARAYRSD  FPPED KSPRDS+GHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 256 SQASLFGLALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGN 315
           +QASL+GLALGTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 316 EPKFYFNDSIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSK 375
           +PK+YFNDSIAIGAFHSMK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAASSIDRKLVS+
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 376 VQLGNNNIFQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGK 435
           VQLGN N FQG+TINTFDL+ KQ PLIYAG+APNIS GFTG++SRFCSRNSVDRNLVKGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 436 ILVCDSILSPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNF 495
           I++CDS+LSP+TF S + AVGVVMND GVKDNARSYPLPSSYL  V G+NIKTYM    F
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 496 PTATIFKSNAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGI 555
           PTATI KSNAVNDTSAP +VSFSSRGPNP T DILKPDLTAPGVEILAAW P A VS G+
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 556 RDSRSTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAE 615
           RDSR+TLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PLN  LN Q E
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 616 FAYGAGHIDPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVW 675
           FAYGAGHI+PL+A +PGL+YDA E+DYV FLCGQGY+TAMVRRL+GD SVCT ANSGRVW
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 676 DLNYPSFALSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGI 735
           DLNYPSFALS+T S+S NQFF RT+TNV ++ STY +K++G P GL+ITV+PPVLSFN I
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660

Query: 736 GDKKSFTLTIDGTIIKTIVSASVVWSDGSHNVRSPITIYIVNKA 780
           G KKSFTLTI G+I ++IVSAS+VWSDG HNVRSPIT+++V  A
Sbjct: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 704

BLAST of CmaCh04G001840 vs. NCBI nr
Match: gi|700195411|gb|KGN50588.1| (hypothetical protein Csa_5G187860 [Cucumis sativus])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 587/740 (79.32%), Postives = 652/740 (88.11%), Query Frame = 1

Query: 41  SSSLIFKL---VILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMRMLEET 100
           SSSLIFKL   ++L LV  LLAS FDS ND RK+YIVY+GNK +DTASTPSHHMRML E 
Sbjct: 2   SSSLIFKLALVLVLGLVSSLLASGFDSKNDDRKIYIVYMGNKPQDTASTPSHHMRMLREV 61

Query: 101 IGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRSWDFMG 160
            GS FAPE+LLHSYKRSFNGFVV+LTEEEA +ISAK+GVVSVFP+ KKH HTTRSWDF+G
Sbjct: 62  TGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSGKKHLHTTRSWDFIG 121

Query: 161 FTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRCNKKII 220
           FT +VPRV QVESDIVVGVLD+GIWPE+PSFSD GYGP PAKWKG CQ  TNF CNKKII
Sbjct: 122 FTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGPIPAKWKGICQNPTNFTCNKKII 181

Query: 221 GARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGVPSARI 280
           GARAYRSDN FP ED  SPRDS GHGTHTASTVAGGLVSQASL+GLALGTARGGVPSARI
Sbjct: 182 GARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARI 241

Query: 281 AVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHSMKNGI 340
           AVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+E ++YFNDSIAIGAFHSMK+GI
Sbjct: 242 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFNDSIAIGAFHSMKHGI 301

Query: 341 LTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTFDLQQK 400
           LTSNSAGNDGPD+ T+RNFSPWSLSVAAS+ DRKLVS+V++GN N++QG+TINTFD   K
Sbjct: 302 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNVYQGYTINTFDPLGK 361

Query: 401 QFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSILSPSTFASFSSAVGV 460
           Q+PLIYAG+APN+ GGFTG+ SRFCS  SVD NLV GKIL+CDSIL+PS F  FS AVGV
Sbjct: 362 QYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGKILLCDSILAPSAFVYFSDAVGV 421

Query: 461 VMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAPIVVSF 520
           VMND GVK  + SYPLPSSYL TV G+ IKTYM SN  PTATIFKS+AVND+SAP +VSF
Sbjct: 422 VMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGVPTATIFKSDAVNDSSAPFIVSF 481

Query: 521 SSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLYNIISGTSMSCPHAT 580
           SSRGPNP TLDILKPDLTAPGVEILAAW P APVS G+ DSR+TLYNIISGTSMSCPH T
Sbjct: 482 SSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGVIDSRTTLYNIISGTSMSCPHVT 541

Query: 581 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPGLVYDA 640
           AAAVYVKTFHPTWSPAAIKSALMTTA PL   +N +AEFAYGAG I+PLKA +PGLVYDA
Sbjct: 542 AAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAEFAYGAGQINPLKAISPGLVYDA 601

Query: 641 NETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFT 700
           NE DYV FLCGQGY++ MV+ L+ D ++C +AN GRVWDLNYPSFALS+TPS+SINQFFT
Sbjct: 602 NEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVWDLNYPSFALSSTPSQSINQFFT 661

Query: 701 RTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTLTIDGTI-IKTIVSA 760
           RTLT+VD+ ASTYTS ILGAP+GLTITV+P VLSF+GIG+KK+FTLTI GTI   TIVSA
Sbjct: 662 RTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGIGEKKTFTLTIQGTIDPTTIVSA 721

Query: 761 SVVWSDGSHNVRSPITIYIV 777
           S+VWSD SH+VRSPITIY+V
Sbjct: 722 SLVWSDSSHDVRSPITIYVV 741

BLAST of CmaCh04G001840 vs. NCBI nr
Match: gi|659123567|ref|XP_008461725.1| (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 1171.0 bits (3028), Expect = 0.0e+00
Identity = 585/755 (77.48%), Postives = 658/755 (87.15%), Query Frame = 1

Query: 27  IFMFPVMTMAGNNASSSLIFKL---VILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDT 86
           +++FP + M  N  SSSLIFKL   ++L LV  LLAS  DS N  RK+YIVY+GNK +DT
Sbjct: 1   MYVFPPLVMTRNIMSSSLIFKLALVLVLGLVFSLLASGLDSENVDRKIYIVYMGNKPQDT 60

Query: 87  ASTPSHHMRMLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNE 146
           ASTPSHHMRML E  GS FAPE+LLHSYKRSFNGFVV+LTEEEA +ISAK+GVVSVFP+ 
Sbjct: 61  ASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEGVVSVFPSG 120

Query: 147 KKHPHTTRSWDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGT 206
           KKH HTTRSWDF+GFT +VPRV QVESDIVVGVLD+GIWPE+PSFSDVGYGP PAKWKG+
Sbjct: 121 KKHLHTTRSWDFLGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDVGYGPIPAKWKGS 180

Query: 207 CQASTNFRCNKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGL 266
           CQ S NF CNKKIIGARAYRSDN FP  D  SPRDS GHGTHTASTVAGGLVSQASL+GL
Sbjct: 181 CQTSANFTCNKKIIGARAYRSDNVFPTADIPSPRDSNGHGTHTASTVAGGLVSQASLYGL 240

Query: 267 ALGTARGGVPSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFND 326
           ALGTARGGVPSARIAVYK+CWSDGC DADILAAFDDAIADGVDIISLSVGG+E ++YFND
Sbjct: 241 ALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEARYYFND 300

Query: 327 SIAIGAFHSMKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNI 386
           SIAIGAFHSMK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAAS+ DRKLVS+V++GN N+
Sbjct: 301 SIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSRVEIGNTNV 360

Query: 387 FQGFTINTFDLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIL 446
           +QG+TINTFD   KQ+PLIYAG+APNI GGFTG+ SRFCS  SVD NLV GKIL+C SI+
Sbjct: 361 YQGYTINTFDPLGKQYPLIYAGDAPNIDGGFTGSISRFCSEGSVDANLVSGKILLCVSIV 420

Query: 447 SPSTFASFSSAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKS 506
           +PS F  FS AVGVVMND GVK  + SYPLPSSYL TV G+ IKTYM SN  PTATIFKS
Sbjct: 421 APSAFVYFSEAVGVVMNDDGVKYPSNSYPLPSSYLQTVDGDAIKTYMASNGVPTATIFKS 480

Query: 507 NAVNDTSAPIVVSFSSRGPNPVTLDILKPDLTAPGVEILAAWPPDAPVSGGIRDSRSTLY 566
           +AVND+ AP VVSFSSRGPNP TLDILKPDLTAPG EILA W P APVS G+ DSR+T+Y
Sbjct: 481 DAVNDSYAPFVVSFSSRGPNPETLDILKPDLTAPGAEILAGWSPIAPVSSGVIDSRTTMY 540

Query: 567 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHI 626
           NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTA PL   +N +AEFAYGAG I
Sbjct: 541 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLKPDINVEAEFAYGAGQI 600

Query: 627 DPLKATNPGLVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFA 686
           +PLKA NPGLVYDANE+DYV FLCGQGY++AMV+ L+ D ++C +AN GRVWDLNYPSFA
Sbjct: 601 NPLKAINPGLVYDANESDYVKFLCGQGYTSAMVQSLSNDNTLCNSANIGRVWDLNYPSFA 660

Query: 687 LSTTPSESINQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPVLSFNGIGDKKSFTL 746
           LS+TPS+SINQFFTRTLT+VD+ ASTYTS ILGAP+GLTITV+P VLSF+G+G+K +FTL
Sbjct: 661 LSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGMGEKNTFTL 720

Query: 747 TIDGTIIKT-IVSASVVWSDGSHNVRSPITIYIVN 778
           TI G+I  T IVSAS+VWSD SH+VRSPITIY+V+
Sbjct: 721 TIQGSIDPTSIVSASLVWSDSSHDVRSPITIYVVS 755