MELO3C020553 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C020553
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Putative progesterone 5-beta-reductase
Location	chr12 : 927279 .. 928798 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCCAAATAATCAACACAAATTTAGATGCCAATGGCGAACTCGTCGCTGTCATATTCGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTCCATTTCATCTCTTGTGATTTGCTCGACCGCCAGTCCGTTCAACAGAATCTCTCCCCGGTTCGCCACGTCACCCACCTCTTTTGGATCACTTGGGCCGCTCAGCTCCGGTTGGATTCCCCGGGTTGCTCCGACCAGAACCGGGCCATGTTGGCCAACGCCCTCGACGCCATTCTCCCCTCCGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGGTCAAACATTATGCTTCGTTGACGAGACTCGCCGCTGGCTGCGGCGGAAGAGAGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGGCGGAGGATGGTGGCGTGGAGTGTTCTCCGGCCCGGTTTGATATTGGGATCTTCGAACCGGACTTTCTTTAATTTCATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCGTTTGTGTTCGGCGGCACGGCAGCATGCTGGGAGGAGGTTTATATCGACGGTTCCGATGCTCGACTCACGGCGGAGCAGCACATGTGGGTCGCAACTAAAGCTGTTGAAATAAATGGTATGTGACTTCTTCACAGGACTAGACAATTTGATGAGCTACACGAACACACCGCTAAATACTTCCTTTTCATTTATACTTATGATCATTTTGTTAAATGAATTAATATTTCATCAATGTCGTCTAGAATTAATTTCGTCAAAATATGGGAGAAATTTTATAAAATGAGTTATGATTGTTACACTTGTTTTACCTATTCATACTCTCCCCACATTTACACACGAAGAAGAGATTTAATCGTTGAAAAACAGGATGAAAACGATAACAATCATCACTTGACCAAAAAGTTTAATAGATAGATGAGAGATGAATTTAATATTATATTCTTAAAAGCACAGCAACTGCAGATGGAGAAGCATTCAACGTTTGCAACGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGACGGTGGCGGAGAAACTTGGAGTGGCGACGACGGCAGAGGAAGAGAAGGAGAGAATGTTTTCGAAGGAGTTTCACTATACGGCGGCGATGGGGGACAAAGGGAAGGTATGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTCGCGACGGAAATGGAAGAGCTGGCGAATTGGGTGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCCGACCGATTTGGGTTCAATGTAAAATACAAGATGTTGGATTCTATCTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

mRNA sequence

ATGGCGGCCCAAATAATCAACACAAATTTAGATGCCAATGGCGAACTCGTCGCTGTCATATTCGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTCCATTTCATCTCTTGTGATTTGCTCGACCGCCAGTCCGTTCAACAGAATCTCTCCCCGGTTCGCCACGTCACCCACCTCTTTTGGATCACTTGGGCCGCTCAGCTCCGGTTGGATTCCCCGGGTTGCTCCGACCAGAACCGGGCCATGTTGGCCAACGCCCTCGACGCCATTCTCCCCTCCGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGGTCAAACATTATGCTTCGTTGACGAGACTCGCCGCTGGCTGCGGCGGAAGAGAGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGGCGGAGGATGGTGGCGTGGAGTGTTCTCCGGCCCGGTTTGATATTGGGATCTTCGAACCGGACTTTCTTTAATTTCATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCGTTTGTGTTCGGCGGCACGGCAGCATGCTGGGAGGAGGTTTATATCGACGGTTCCGATGCTCGACTCACGGCGGAGCAGCACATGTGGGTCGCAACTAAAGCTGTTGAAATAAATGCAACTGCAGATGGAGAAGCATTCAACGTTTGCAACGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGACGGTGGCGGAGAAACTTGGAGTGGCGACGACGGCAGAGGAAGAGAAGGAGAGAATGTTTTCGAAGGAGTTTCACTATACGGCGGCGATGGGGGACAAAGGGAAGGTATGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTCGCGACGGAAATGGAAGAGCTGGCGAATTGGGTGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCCGACCGATTTGGGTTCAATGTAAAATACAAGATGTTGGATTCTATCTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

Coding sequence (CDS)

ATGGCGGCCCAAATAATCAACACAAATTTAGATGCCAATGGCGAACTCGTCGCTGTCATATTCGGAGTCACCGGTTTGGTCGGAAAACAGTTGGCCAAAACCCTTCTCTCCACCGCCGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAATGTCTCTCCCATCTCCCATCCCAAATTCCATTTCATCTCTTGTGATTTGCTCGACCGCCAGTCCGTTCAACAGAATCTCTCCCCGGTTCGCCACGTCACCCACCTCTTTTGGATCACTTGGGCCGCTCAGCTCCGGTTGGATTCCCCGGGTTGCTCCGACCAGAACCGGGCCATGTTGGCCAACGCCCTCGACGCCATTCTCCCCTCCGCGCCGGCCCTCCGCCACGTCTCTCTACAGACCGGGGTCAAACATTATGCTTCGTTGACGAGACTCGCCGCTGGCTGCGGCGGAAGAGAGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAACAATTTTTATTACGTGTTGGAAGATCTCCTCCGTGAGCGGTTGTCCTGTGGGCGGAGGATGGTGGCGTGGAGTGTTCTCCGGCCCGGTTTGATATTGGGATCTTCGAACCGGACTTTCTTTAATTTCATGGGGAGTTTGTGTGTTTATGGTGCCATCTGTAAGAAGTTGAAGATGCCGTTTGTGTTCGGCGGCACGGCAGCATGCTGGGAGGAGGTTTATATCGACGGTTCCGATGCTCGACTCACGGCGGAGCAGCACATGTGGGTCGCAACTAAAGCTGTTGAAATAAATGCAACTGCAGATGGAGAAGCATTCAACGTTTGCAACGGGTGGAGTTTCCGGTGGAAGGAGATATGGGGGACGGTGGCGGAGAAACTTGGAGTGGCGACGACGGCAGAGGAAGAGAAGGAGAGAATGTTTTCGAAGGAGTTTCACTATACGGCGGCGATGGGGGACAAAGGGAAGGTATGGGCGGAGATAGTGGAGGAGGAAGGGTTGGTCGCGACGGAAATGGAAGAGCTGGCGAATTGGGTGTTCTTAGACACATTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCTAAGTCCGACCGATTTGGGTTCAATGTAAAATACAAGATGTTGGATTCTATCTTGTATTGGATTGATGTCATGAGAAATGACAAATTGATTCCTTCTTAA

Protein sequence

MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS*

Homology

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BLAST of MELO3C020553 vs. Swiss-Prot
Match: VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 9.1e-63
Identity = 152/382 (39.79%), Postives = 203/382 (53.14%), Query Frame = 1


Query: 15  ELVAVIFGVTGLVGKQLAKTL--LSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+ L    T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSL 134
            +  +  LSP+  VTH+F++TW  +   +S  C + N +ML N L AI+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWS 194
           QTG KHY  L       G R +  + ED PR +   NFYY  ED+L E +      V WS
Sbjct: 146 QTGTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTWS 205

Query: 195 VLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAE 254
           + RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L AE
Sbjct: 206 IHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADLIAE 265

Query: 255 QHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEF 314
           Q +W A     ++  A  EAFN  N   F+WK +W  +AE+ G+     EE      K  
Sbjct: 266 QQIWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-----GKNL 325

Query: 315 HYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNV 374
                M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF  
Sbjct: 326 GLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLG 385

Query: 375 KYKMLDSILYWIDVMRNDKLIP 393
                +S + WID  +  K++P
Sbjct: 386 FRNSNNSFISWIDKYKAFKIVP 388

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BLAST of MELO3C020553 vs. Swiss-Prot
Match: 5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.2e-58
Identity = 147/380 (38.68%), Postives = 198/380 (52.11%), Query Frame = 1


Query: 17  VAVIFGVTGLVGKQLAKTL--LSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLDRQ 76
           VA+I GVTG++G  LA+ L    T G  WKVYGVARR        +P  +++ CD+ D  
Sbjct: 28  VALIVGVTGIIGNSLAEILPLADTPGGPWKVYGVARRTRPAWHEDNP-INYVQCDISDPD 87

Query: 77  SVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQT 136
             Q  LSP+  VTH+F++TWA +   +   C + N  M  N LDA++P+ P L+H+SLQT
Sbjct: 88  DSQAKLSPLTDVTHVFYVTWANR-STEQENC-EANSKMFRNVLDAVIPNCPNLKHISLQT 147

Query: 137 GVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVL 196
           G KHY      + G     +  Y ED PR +   NFYY LED++ E +   +  + WSV 
Sbjct: 148 GRKHYMGPFE-SYGKIESHDPPYTEDLPRLKY-MNFYYDLEDIMLEEVE-KKEGLTWSVH 207

Query: 197 RPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQH 256
           RPG I G S  +  N +G+LCVY AICK       F G  A W+  Y D SDA L AE H
Sbjct: 208 RPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAWDG-YSDCSDADLIAEHH 267

Query: 257 MWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHY 316
           +W A     ++  A  EAFNV NG  F+WK  W  +AE+ GV     EE       +   
Sbjct: 268 IWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVGCGEYEE-----GVDLKL 327

Query: 317 TAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKY 376
              M  K  VW EIV E GL  T+++++  W F D +    E  + S  KS   GF    
Sbjct: 328 QDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFR 387

Query: 377 KMLDSILYWIDVMRNDKLIP 393
              ++ + WID  +  K++P
Sbjct: 388 NSKNAFISWIDKAKAYKIVP 389

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BLAST of MELO3C020553 vs. Swiss-Prot
Match: IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.4e-55
Identity = 139/380 (36.58%), Postives = 197/380 (51.84%), Query Frame = 1


Query: 17  VAVIFGVTGLVGKQLAKTLL--STAG--WKVYGVARRPDNVSPISHPKFHFISCDLLDRQ 76
           VA++ GVTG+VG  LA+ L    T G  WKVYGVARRP  V     P   +I CD+ D Q
Sbjct: 31  VALVVGVTGIVGSSLAEVLKLPDTPGGPWKVYGVARRPCPVWLAKKP-VEYIQCDVSDNQ 90

Query: 77  SVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQT 136
                LSP++ +TH+F+++W     + S  C   N  M  N L++++P+A  L+HV LQT
Sbjct: 91  ETISKLSPLKDITHIFYVSW-----IGSEDCQT-NATMFKNILNSVIPNASNLQHVCLQT 150

Query: 137 GVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVL 196
           G+KHY  +    +     +   + ED PR  +  NFY+ LED+L E    G+  + WSV 
Sbjct: 151 GIKHYFGIFEEGSKVVPHDS-PFTEDLPRL-NVPNFYHDLEDILYE--ETGKNNLTWSVH 210

Query: 197 RPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQH 256
           RP L+ G S  +  N + +LCVY  ICK      V+ G+   W   Y D  DA L AE  
Sbjct: 211 RPALVFGFSPCSMMNIVSTLCVYATICKHENKALVYPGSKNSW-NCYADAVDADLVAEHE 270

Query: 257 MWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHY 316
           +W A     ++  A  +  N  NG  F+WK IW  +AE+ G+      E +   S     
Sbjct: 271 IWAA-----VDPKAKNQVLNCNNGDVFKWKHIWKKLAEEFGIEMVGYVEGKEQVS----L 330

Query: 317 TAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKY 376
              M DK +VW EIV++  LV T+++E+A + F D  F   E +++S  KS   GF    
Sbjct: 331 AELMKDKDQVWDEIVKKNNLVPTKLKEIAAFWFADIAF-CSENLISSMNKSKELGFLGFR 388

Query: 377 KMLDSILYWIDVMRNDKLIP 393
             + S +  ID MR+ + IP
Sbjct: 391 NSMKSFVSCIDKMRDYRFIP 388

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BLAST of MELO3C020553 vs. Swiss-Prot
Match: YJ72_SCHPO (Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC757.02c PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 5.4e-15
Identity = 95/367 (25.89%), Postives = 158/367 (43.05%), Query Frame = 1


Query: 17  VAVIFGVTGLVGKQLAKTLLSTAGWK-VYGVARRPDNVSPISHPKFHFISCDLLDRQ--- 76
           VA++ G TGL G  + K L      K ++ ++R   +  P    K    S DLL+ +   
Sbjct: 7   VAIVTGATGLNGAAIIKRLSEDDNCKTIHCISRSLKDEYP---RKIKHHSIDLLNEEPKD 66

Query: 77  -SVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAI-LPSAPALRHVSL 136
            + + +L  V+ + + ++  +  +   +     + N  ML N + A+ L S   LR V L
Sbjct: 67  IAKKFSLEGVKGINYAYFAAYKEENNEEK--LCEVNGNMLRNFVQALELTSIQTLRRVIL 126

Query: 137 QTGVKHYASLTRLAAGCGGREEVVYCEDS---PRAESGN-NFYYVLEDLLRERLSCGRRM 196
            TG+K Y           G   +   E     P   SG  NFYYV ED+L+E  S G++ 
Sbjct: 127 TTGLKFYGLHL-------GEVRLPMIETDIRVPETFSGTPNFYYVQEDILKE-FSNGKKW 186

Query: 197 VAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI---DGS 256
             +++  P  I G S  ++ N   ++ +Y  +C++L  PF F G     E+ Y+   D S
Sbjct: 187 -DYTIAMPNDICGVSKGSYMNEAFTIALYALVCRELHEPFRFPGN----EKFYLGFDDIS 246

Query: 257 DARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKE 316
            ++L A+  +W+  K     A    E FN+ NG    W   W  +AE  GV     E  +
Sbjct: 247 YSKLIADFQLWMTFK-----AECSEEKFNIVNGDIHSWSRTWPKIAEYFGV-----EVPK 306

Query: 317 RMFSKEFHYTAAMGDKGKVWAEIVEEE-GLVATEMEELANWVFLDTLFRLPEKMVASRAK 370
             F+ +F  +  +         + E+E G+  T   ++ N + L    +  +   A R  
Sbjct: 307 NQFATDFTLSTEVTLSTPSPINLYEKELGIKHTPNSKIINQISLQQWVKQKKVQDAWRTI 345

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BLAST of MELO3C020553 vs. TrEMBL
Match: A0A0A0LVI2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G530150 PE=4 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 6.9e-219
Identity = 379/396 (95.71%), Postives = 384/396 (96.97%), Query Frame = 1


Query: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGG--REEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGCGG    EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300
           YIDGSDARLTAEQH+WVATKAVEINATADGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 394
           ASRAKSDR GFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

[Close]

BLAST of MELO3C020553 vs. TrEMBL
Match: M5W676_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021134mg PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.6e-119
Identity = 224/392 (57.14%), Postives = 276/392 (70.41%), Query Frame = 1


Query: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAA+       A  E VA IFGVTGLVGK LAK L+S + WKVYG+AR P  +S      
Sbjct: 1   MAAEETKRPEAAGIEHVAAIFGVTGLVGKVLAKELISKSKWKVYGIARNPMIISNQQCSN 60

Query: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120
           FHFI CDLL+    QQ LS ++ VTH+FW+TWA+Q +LDS  C +QN+AM++N L+AI+P
Sbjct: 61  FHFIQCDLLNPLETQQRLSLLQDVTHVFWVTWASQYQLDSIECCEQNKAMMSNVLNAIVP 120

Query: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180
           +A AL+HVSLQTG+KHY S   L       E   Y E+ PR ++G NFYYVLEDLL+ERL
Sbjct: 121 TAKALKHVSLQTGMKHYVS---LQGPFDEAEARYYNENGPRVKAGCNFYYVLEDLLKERL 180

Query: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240
                 +AWSV RPGL++GSS+RT +NFMGSLCVYG ICK L +PFVFGGT  CWEE  I
Sbjct: 181 D---GKIAWSVHRPGLLIGSSSRTLYNFMGSLCVYGTICKHLNLPFVFGGTKKCWEETCI 240

Query: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300
           DGSDARL AEQH+W AT       + DG++FN  NG SF WKEIW  +AEK G+    E 
Sbjct: 241 DGSDARLVAEQHIWAATNDDVNVQSTDGQSFNAINGPSFTWKEIWTALAEKFGL----EV 300

Query: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360
             E  FS++F Y+ AM DK +VW EIV +E ++ TEME+LANW FLD LFR P K++ +R
Sbjct: 301 VPENAFSEDFWYSKAMADKKEVWKEIVAKERMMKTEMEDLANWAFLDVLFRCPFKLLGTR 360

Query: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIP 393
            K D+ GF V+YK LDSILYWID MR++KLIP
Sbjct: 361 NKVDQLGFTVRYKTLDSILYWIDCMRDEKLIP 382

[Close]

BLAST of MELO3C020553 vs. TrEMBL
Match: B9SI19_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0612220 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 4.8e-119
Identity = 228/385 (59.22%), Postives = 278/385 (72.21%), Query Frame = 1


Query: 11  DANGEL--VAVIFGVTGLVGKQLAKTLLSTAGW-KVYGVARRPDNVSPISHPKFHFISCD 70
           D+NGE   VAVIFG TGLVG++L + L+S + W KVYGVARR ++  PI +P +HFISCD
Sbjct: 9   DSNGETEAVAVIFGATGLVGRELVRRLISKSKWKKVYGVARRFESF-PIQNPNYHFISCD 68

Query: 71  LLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRH 130
           LL  Q  Q+ LS ++  TH+FW+TWA Q  LDS  C DQN AM++NAL+AIL    AL+H
Sbjct: 69  LLIPQETQKKLSVIQDATHMFWVTWAGQFPLDSKECCDQNMAMMSNALNAILQQTNALQH 128

Query: 131 VSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMV 190
           VSLQTG+KHY SL +   G        Y ED PRA  G NFYYVLED L+ERL+     V
Sbjct: 129 VSLQTGMKHYVSLQQ---GHNANRVCFYGEDCPRASGGCNFYYVLEDFLKERLA---GKV 188

Query: 191 AWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARL 250
           AWSVLRPGL++GSSNRT +N MGSLCVYGAICK L + FVFGGT  CWEE  IDGSDARL
Sbjct: 189 AWSVLRPGLLMGSSNRTMYNVMGSLCVYGAICKYLHLAFVFGGTMECWEEACIDGSDARL 248

Query: 251 TAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFS 310
            AEQH+W AT   EI++T+ G+AFN  NG SF WKEIW  + +KL V     E  + MF 
Sbjct: 249 VAEQHIWAATNG-EISSTS-GQAFNAINGPSFTWKEIWPILGKKLEV-----EMPQDMFL 308

Query: 311 KEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFG 370
           ++F ++ A+ +K  VW EIVE+EGL+ TEME+LANW FLD LFR P KM+ SR KSDR G
Sbjct: 309 EDFWFSKAVSNKKDVWKEIVEKEGLLQTEMEDLANWEFLDALFRCPSKMLGSREKSDRLG 368

Query: 371 FNVKYKMLDSILYWIDVMRNDKLIP 393
           F ++ K +DS+LYWID MR+DKLIP
Sbjct: 369 FTMRCKTMDSMLYWIDSMRDDKLIP 379

[Close]

BLAST of MELO3C020553 vs. TrEMBL
Match: A0A067LNQ3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08472 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.1e-118
Identity = 218/376 (57.98%), Postives = 276/376 (73.40%), Query Frame = 1


Query: 17  VAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQ 76
           VA+IFGVTGLVG+++ + LL+ + W+VYG+ARR ++  PI +P +HFISCDLL+ +  ++
Sbjct: 11  VAIIFGVTGLVGREIMRGLLAKSKWEVYGIARRFESF-PIKNPNYHFISCDLLNAKETEK 70

Query: 77  NLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVKH 136
            LS V+  TH+FW+TW  Q  LDS  C +QN+ M++NAL++IL +   L+HVSLQTG+KH
Sbjct: 71  KLSIVQDATHVFWVTWTGQFPLDSKECCEQNKIMMSNALNSILSTTKTLKHVSLQTGMKH 130

Query: 137 YASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGL 196
           Y SL +       RE  +Y ED PRA  G NFYYVLEDLL+ERL+  +  VAWSV+RPGL
Sbjct: 131 YLSLQQKDNNVE-REVCLYDEDCPRASQGYNFYYVLEDLLKERLASNK--VAWSVIRPGL 190

Query: 197 ILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWVA 256
           ++GSSNRT +N MGSLCVYGAICK L +PFVFGGT  CWEEV IDGSDA L AEQH+W A
Sbjct: 191 LMGSSNRTLYNVMGSLCVYGAICKYLNLPFVFGGTRECWEEVCIDGSDAVLVAEQHIWAA 250

Query: 257 TKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAAM 316
           T   E+ +T DG+AFN  NG SF WKEIW  + +K  V     +  + MF K+F +  AM
Sbjct: 251 TND-ELYST-DGQAFNSINGTSFTWKEIWPILGKKFEV-----QVPKNMFLKDFWFEKAM 310

Query: 317 GDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLD 376
            DK KVWAEIV +EGL  TEME+LANW F+D LFR P KM+ SRAK+D+ GF ++ K LD
Sbjct: 311 SDKNKVWAEIVVKEGLAETEMEDLANWEFIDILFRCPMKMLGSRAKADQLGFTMRCKTLD 370

Query: 377 SILYWIDVMRNDKLIP 393
           SILYWID MR+D LIP
Sbjct: 371 SILYWIDSMRDDNLIP 375

[Close]

BLAST of MELO3C020553 vs. TrEMBL
Match: A0A022RYY0_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a018609mg PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.1e-118
Identity = 217/377 (57.56%), Postives = 263/377 (69.76%), Query Frame = 1


Query: 17  VAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQ 76
           VA IFGVTGLVGK++A+ LLST  WKVYG++RRP+ V P    ++ FI CDLL+    ++
Sbjct: 11  VAAIFGVTGLVGKEIARMLLSTCDWKVYGISRRPEKVGPADVERYRFIPCDLLNPLETRK 70

Query: 77  NLSPVRH-VTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVK 136
           NLSP+   VTH+FW+TWA+Q  LDS  C +QNRAM+AN L+A+LP A ALRHVSLQTG K
Sbjct: 71  NLSPILDDVTHIFWVTWASQFSLDSSDCYEQNRAMMANVLNALLPKAKALRHVSLQTGTK 130

Query: 137 HYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPG 196
           HY SLT    G    +   Y E  PRA  G+NFYY LEDLL+ERL      VAWS+ RPG
Sbjct: 131 HYVSLT----GSSDPQVGHYDEKCPRACLGHNFYYALEDLLQERLPSHN--VAWSIHRPG 190

Query: 197 LILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWV 256
           LI+G S++T +NF+GS+CVYGAICK L +PFVFGGT  CWEE+Y+D SDARL AEQH+WV
Sbjct: 191 LIMGCSDKTLYNFIGSICVYGAICKHLNLPFVFGGTKECWEEMYLDASDARLVAEQHIWV 250

Query: 257 ATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAA 316
            T     +    G+AFN  NG S+ WKEIW  +  K GV      E    FS +F + + 
Sbjct: 251 GTNDKITSDNLQGQAFNAINGESYTWKEIWPAIGAKFGVNV---HENVPFFSGDFVFASE 310

Query: 317 MGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKML 376
           MGDKG VW EIV +EGLV T + +LANW FLD LFR P K++ +R K D  GF  KYK L
Sbjct: 311 MGDKGGVWKEIVAKEGLVDTNLMDLANWDFLDLLFRCPLKILGTRFKIDSMGFETKYKTL 370

Query: 377 DSILYWIDVMRNDKLIP 393
           DSILYWIDVMR DK IP
Sbjct: 371 DSILYWIDVMRADKFIP 378

[Close]

BLAST of MELO3C020553 vs. TAIR10
Match: AT5G58750.1 (AT5G58750.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 408.7 bits (1049), Expect = 4.1e-114
Identity = 210/381 (55.12%), Postives = 262/381 (68.77%), Query Frame = 1


Query: 15  ELVAVIFGVTGLVGKQLAKTLL-STAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQS 74
           E VA+IFGVTGLVG+++ KTLL S  GW++YGVAR P+  S      ++FISCDLL+   
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINSMTK--MYNFISCDLLNASE 75

Query: 75  VQQNLSPVRH-VTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQT 134
            +Q LSP++  V+H+FW+TW+ +  LD+  C  QN+ ML NALDAILP+A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GVKHYASLTRLAAGCG-GREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSV 194
           G+KHY SL       G G     Y E+ PR  SG NFYYVLEDLL+E+++  R  V WSV
Sbjct: 136 GMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKIT--RSSVVWSV 195

Query: 195 LRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQ 254
            RPGL++GSS+RT +NFMGSLCVYGA+CK L +PFVFGGT  CWEE YIDGSD+ L AEQ
Sbjct: 196 QRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQ 255

Query: 255 HMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFH 314
           H++ AT          GEAFN  NG  F WKEIW  + +KLGV    +  +  MF + F 
Sbjct: 256 HIFAATSG---KVREKGEAFNAINGVGFTWKEIWPEIGKKLGV----QVNETTMFDEGFW 315

Query: 315 YTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVK 374
           +   M ++  VW EIV +E LV TE+E+LANW FLD LFR P K++  R K DRFGF  K
Sbjct: 316 FGREMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRK 375

Query: 375 YKMLDSILYWIDVMRNDKLIP 393
           Y+ LDS+LYWIDVMR++KLIP
Sbjct: 376 YRTLDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of MELO3C020553 vs. TAIR10
Match: AT4G24220.1 (AT4G24220.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 242.3 bits (617), Expect = 5.1e-64
Identity = 152/382 (39.79%), Postives = 203/382 (53.14%), Query Frame = 1


Query: 15  ELVAVIFGVTGLVGKQLAKTL--LSTAG--WKVYGVARRPDNVSPISHPKFHFISCDLLD 74
           E VA+I GVTG+VG  LA+ L    T G  WKVYGVARRP       HP   +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHP-IDYIQCDVSD 85

Query: 75  RQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSL 134
            +  +  LSP+  VTH+F++TW  +   +S  C + N +ML N L AI+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWS 194
           QTG KHY  L       G R +  + ED PR +   NFYY  ED+L E +      V WS
Sbjct: 146 QTGTKHY--LGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTWS 205

Query: 195 VLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAE 254
           + RP +I G S  +  N +G+LCVY AICK    P +F G+   WE  ++  SDA L AE
Sbjct: 206 IHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEG-FMTASDADLIAE 265

Query: 255 QHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEF 314
           Q +W A     ++  A  EAFN  N   F+WK +W  +AE+ G+     EE      K  
Sbjct: 266 QQIWAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE-----GKNL 325

Query: 315 HYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNV 374
                M  K +VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF  
Sbjct: 326 GLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLG 385

Query: 375 KYKMLDSILYWIDVMRNDKLIP 393
                +S + WID  +  K++P
Sbjct: 386 FRNSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of MELO3C020553 vs. NCBI nr
Match: gi|659115079|ref|XP_008457376.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo])

HSP 1 Score: 802.4 bits (2071), Expect = 3.6e-229
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 1


Query: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120
           FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180
           SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240
           SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI
Sbjct: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300
           DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE
Sbjct: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300

Query: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360
           EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 394
           AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]

BLAST of MELO3C020553 vs. NCBI nr
Match: gi|659115081|ref|XP_008457377.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo])

HSP 1 Score: 792.0 bits (2044), Expect = 4.9e-226
Identity = 390/393 (99.24%), Postives = 390/393 (99.24%), Query Frame = 1


Query: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120
           FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180
           SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240
           SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI
Sbjct: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300
           DGSDARLTAEQHMWVATKAVEIN   DGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE
Sbjct: 241 DGSDARLTAEQHMWVATKAVEIN---DGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300

Query: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360
           EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 394
           AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

[Close]

BLAST of MELO3C020553 vs. NCBI nr
Match: gi|449455156|ref|XP_004145319.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus])

HSP 1 Score: 767.7 bits (1981), Expect = 9.9e-219
Identity = 379/396 (95.71%), Postives = 384/396 (96.97%), Query Frame = 1


Query: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60
           MA +IINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120
           FHFISCDLLDR+SVQQNLSPVRHVTHLFWITWAAQLRLDSP CSDQNRAMLANALDAILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGG--REEVVYCEDSPRAESGNNFYYVLEDLLRE 180
           SAPALRHVSLQTG+KHYASLTRLAAGCGG    EVVYCEDSPRAESGNNFYYVLEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240
           RLSCGRRMVAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATT- 300
           YIDGSDARLTAEQH+WVATKAVEINATADGEAFNVCNGWSFRWKEIWG VAEKLGVATT 
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMV 360
           AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLV TEMEELANW FLDTLFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 394
           ASRAKSDR GFNVKYKMLDS+LYWIDVMRNDKLIPS
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIPS 396

[Close]

BLAST of MELO3C020553 vs. NCBI nr
Match: gi|720053693|ref|XP_010272810.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Nelumbo nucifera])

HSP 1 Score: 445.7 bits (1145), Expect = 8.6e-122
Identity = 224/389 (57.58%), Postives = 282/389 (72.49%), Query Frame = 1


Query: 6   INTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFIS 65
           I+T  D   E VA+IFGVTGLVGK++A  LL+ +GWKVYG+AR+PD  +PI H  + FIS
Sbjct: 15  ISTKKDV-AESVALIFGVTGLVGKEIANNLLAKSGWKVYGIARKPDFFTPIQHRNYRFIS 74

Query: 66  CDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPAL 125
           CDLLD     Q LS +   TH+FW+TWA+Q  LDS  C DQN+AM++NALDAILP A AL
Sbjct: 75  CDLLDPVQTVQKLSSLNGATHIFWVTWASQFPLDSSECCDQNKAMMSNALDAILPKAKAL 134

Query: 126 RHVSLQTGVKHYASLTRLAAGCGGREEVV--YCEDSPRAESGNNFYYVLEDLLRERLSCG 185
           +HVSLQTG KHY SL+      G  +  V  Y E+SPR   G+NFYY+LEDLL E+L   
Sbjct: 135 KHVSLQTGTKHYVSLS------GPFDNTVRRYDEESPRVNEGHNFYYMLEDLLIEKL--- 194

Query: 186 RRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGS 245
           R  V+WSV RPGLILGSS+RT FNF+GSLC+YG ICK L +PFVFGG   CWEEVYIDGS
Sbjct: 195 RGKVSWSVHRPGLILGSSHRTAFNFIGSLCIYGTICKHLHLPFVFGGARECWEEVYIDGS 254

Query: 246 DARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKE 305
           DARL AEQH+WV+T   +  ++ DG+AFN  NG SF WK+IW  +A K G+     +  E
Sbjct: 255 DARLVAEQHIWVSTN--DNVSSTDGQAFNAVNGPSFTWKQIWPALAVKFGL-----QAAE 314

Query: 306 RMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKS 365
            MFS++F ++ +M DK  VW +IV +EGL  T++ +LANW FLD LFR P K++A+R K 
Sbjct: 315 DMFSQDFLFSTSMADKESVWEDIVVKEGLRPTKIGDLANWTFLDVLFRFPTKLLAARDKV 374

Query: 366 DRFGFNVKYKMLDSILYWIDVMRNDKLIP 393
           DR GF + Y+MLDSILYW+D +R++KLIP
Sbjct: 375 DRLGFTMTYEMLDSILYWVDCIRDEKLIP 386

[Close]

BLAST of MELO3C020553 vs. NCBI nr
Match: gi|720053698|ref|XP_010272811.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Nelumbo nucifera])

HSP 1 Score: 445.7 bits (1145), Expect = 8.6e-122
Identity = 224/389 (57.58%), Postives = 282/389 (72.49%), Query Frame = 1


Query: 6   INTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFIS 65
           I+T  D   E VA+IFGVTGLVGK++A  LL+ +GWKVYG+AR+PD  +PI H  + FIS
Sbjct: 12  ISTKKDV-AESVALIFGVTGLVGKEIANNLLAKSGWKVYGIARKPDFFTPIQHRNYRFIS 71

Query: 66  CDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILPSAPAL 125
           CDLLD     Q LS +   TH+FW+TWA+Q  LDS  C DQN+AM++NALDAILP A AL
Sbjct: 72  CDLLDPVQTVQKLSSLNGATHIFWVTWASQFPLDSSECCDQNKAMMSNALDAILPKAKAL 131

Query: 126 RHVSLQTGVKHYASLTRLAAGCGGREEVV--YCEDSPRAESGNNFYYVLEDLLRERLSCG 185
           +HVSLQTG KHY SL+      G  +  V  Y E+SPR   G+NFYY+LEDLL E+L   
Sbjct: 132 KHVSLQTGTKHYVSLS------GPFDNTVRRYDEESPRVNEGHNFYYMLEDLLIEKL--- 191

Query: 186 RRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGS 245
           R  V+WSV RPGLILGSS+RT FNF+GSLC+YG ICK L +PFVFGG   CWEEVYIDGS
Sbjct: 192 RGKVSWSVHRPGLILGSSHRTAFNFIGSLCIYGTICKHLHLPFVFGGARECWEEVYIDGS 251

Query: 246 DARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEKE 305
           DARL AEQH+WV+T   +  ++ DG+AFN  NG SF WK+IW  +A K G+     +  E
Sbjct: 252 DARLVAEQHIWVSTN--DNVSSTDGQAFNAVNGPSFTWKQIWPALAVKFGL-----QAAE 311

Query: 306 RMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAKS 365
            MFS++F ++ +M DK  VW +IV +EGL  T++ +LANW FLD LFR P K++A+R K 
Sbjct: 312 DMFSQDFLFSTSMADKESVWEDIVVKEGLRPTKIGDLANWTFLDVLFRFPTKLLAARDKV 371

Query: 366 DRFGFNVKYKMLDSILYWIDVMRNDKLIP 393
           DR GF + Y+MLDSILYW+D +R++KLIP
Sbjct: 372 DRLGFTMTYEMLDSILYWVDCIRDEKLIP 383

The following BLAST results are available for this feature:

BLAST of MELO3C020553 vs. Swiss-Prot


Match Name	E-value	Identity	Description
VEP1_ARATH	9.1e-63	39.79	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 S...	[more]
5BPOR_DIGLA	5.2e-58	38.68	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1	[more]
IRIS_CATRO	2.4e-55	36.58	Iridoid synthase OS=Catharanthus roseus PE=1 SV=1	[more]
YJ72_SCHPO	5.4e-15	25.89	Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of MELO3C020553 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LVI2_CUCSA	6.9e-219	95.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G530150 PE=4 SV=1	[more]
M5W676_PRUPE	1.6e-119	57.14	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021134mg PE=4 SV=1	[more]
B9SI19_RICCO	4.8e-119	59.22	Putative uncharacterized protein OS=Ricinus communis GN=RCOM_0612220 PE=4 SV=1	[more]
A0A067LNQ3_JATCU	3.1e-118	57.98	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08472 PE=4 SV=1	[more]
A0A022RYY0_ERYGU	3.1e-118	57.56	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a018609mg PE=4 SV=1	[more]

BLAST of MELO3C020553 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G58750.1	4.1e-114	55.12	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	5.1e-64	39.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of MELO3C020553 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659115079\|ref\|XP_008457376.1\|	3.6e-229	100.00	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo]	[more]
gi\|659115081\|ref\|XP_008457377.1\|	4.9e-226	99.24	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo]	[more]
gi\|449455156\|ref\|XP_004145319.1\|	9.9e-219	95.71	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus]	[more]
gi\|720053693\|ref\|XP_010272810.1\|	8.6e-122	57.58	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Nelumbo nucifer...	[more]
gi\|720053698\|ref\|XP_010272811.1\|	8.6e-122	57.58	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Nelumbo nucifer...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001509	Epimerase_deHydtase
IPR016040	NAD(P)-bd_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0050662	coenzyme binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050662	coenzyme binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
MU59955	melon EST collection version 4.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C020553T1	MELO3C020553T1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
MU59955	MU59955	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase, N-terminal domain	PFAM	PF01370	Epimerase	coord: 19..87 score: 2.
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 19..294 score: 3.0
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 17..296 score: 5.04
None	No IPR available	PANTHER	PTHR32487	FAMILY NOT NAMED	coord: 15..393 score: 2.0E
None	No IPR available	PANTHER	PTHR32487:SF7	SUBFAMILY NOT NAMED	coord: 15..393 score: 2.0E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C020553	Csa1G530150	Cucumber (Chinese Long) v2	cumeB028
MELO3C020553	Cla008168	Watermelon (97103) v1	mewmB146
MELO3C020553	ClCG03G000790	Watermelon (Charleston Gray)	mewcgB149
MELO3C020553	Cla97C03G051600	Watermelon (97103) v2	mewmbB153
MELO3C020553	Bhi08G000424	Wax gourd	mewgoB161
MELO3C020553	CSPI01G23840	Wild cucumber (PI 183967)	cpimeB025
MELO3C020553	Cucsa.147030	Cucumber (Gy14) v1	cgymeB243
MELO3C020553	CmaCh13G000690	Cucurbita maxima (Rimu)	cmameB194
MELO3C020553	CmaCh01G006600	Cucurbita maxima (Rimu)	cmameB391
MELO3C020553	CmoCh01G006910	Cucurbita moschata (Rifu)	cmomeB382
MELO3C020553	CmoCh13G000580	Cucurbita moschata (Rifu)	cmomeB181
MELO3C020553	Lsi02G012870	Bottle gourd (USVL1VR-Ls)	lsimeB174
MELO3C020553	Cp4.1LG20g08120	Cucurbita pepo (Zucchini)	cpemeB479
MELO3C020553	Cp4.1LG02g11730	Cucurbita pepo (Zucchini)	cpemeB496
MELO3C020553	CsGy1G023320	Cucumber (Gy14) v2	cgybmeB023
MELO3C020553	Carg12426	Silver-seed gourd	carmeB0449
MELO3C020553	Carg20604	Silver-seed gourd	carmeB0388
MELO3C020553	CsaV3_1G036150	Cucumber (Chinese Long) v3	cucmeB025

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C020553	Silver-seed gourd	carmeB0159
MELO3C020553	Silver-seed gourd	carmeB0663
MELO3C020553	Silver-seed gourd	carmeB0971
MELO3C020553	Cucumber (Chinese Long) v3	cucmeB465
MELO3C020553	Watermelon (97103) v2	mewmbB148
MELO3C020553	Watermelon (97103) v2	mewmbB160
MELO3C020553	Wax gourd	mewgoB152
MELO3C020553	Melon (DHL92) v3.5.1	memeB069
MELO3C020553	Melon (DHL92) v3.5.1	memeB072
MELO3C020553	Cucumber (Gy14) v1	cgymeB220
MELO3C020553	Cucumber (Gy14) v1	cgymeB313
MELO3C020553	Cucurbita maxima (Rimu)	cmameB216
MELO3C020553	Cucurbita maxima (Rimu)	cmameB666
MELO3C020553	Cucurbita maxima (Rimu)	cmameB667
MELO3C020553	Cucurbita maxima (Rimu)	cmameB669
MELO3C020553	Cucurbita maxima (Rimu)	cmameB750
MELO3C020553	Cucurbita moschata (Rifu)	cmomeB201
MELO3C020553	Cucurbita moschata (Rifu)	cmomeB658
MELO3C020553	Cucurbita moschata (Rifu)	cmomeB661
MELO3C020553	Cucurbita moschata (Rifu)	cmomeB662
MELO3C020553	Cucurbita moschata (Rifu)	cmomeB742
MELO3C020553	Wild cucumber (PI 183967)	cpimeB180
MELO3C020553	Wild cucumber (PI 183967)	cpimeB454
MELO3C020553	Cucumber (Chinese Long) v2	cumeB185
MELO3C020553	Cucumber (Chinese Long) v2	cumeB459
MELO3C020553	Watermelon (Charleston Gray)	mewcgB128
MELO3C020553	Watermelon (Charleston Gray)	mewcgB155
MELO3C020553	Watermelon (97103) v1	mewmB145
MELO3C020553	Watermelon (97103) v1	mewmB175
MELO3C020553	Cucurbita pepo (Zucchini)	cpemeB390
MELO3C020553	Bottle gourd (USVL1VR-Ls)	lsimeB245
MELO3C020553	Cucumber (Gy14) v2	cgybmeB398