Carg12426 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg12426
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	3-oxo-delta(4,5)-steroid 5-beta-reductase
Location	Cucurbita_argyrosperma_scaffold_074 : 907136 .. 908411 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACTTGTCGCCGTCATTTTCGGAGTCACCGGTTTGGTCGGAAAAGAGTTAGCAAAAGCCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATACTAATTTTCAATTCATTTCCTGCGATTTGCTCGACCGCTCTTCCGCTAACCGAAACCTCTCGCCGGTCCGCCACGTCACACATCTTTTCTGGGTCACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTCGACGTCATTCTCCCGGCGGCGCCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGCCGCCGTCGGAGGCGGCGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCTGGAGGTGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACAGTACGCCACTCTTTCACTATTAATTTATTTTTAATAATTAATTTCAATTTTCCATAAAAATATTTATATAAAATATTTTATGTGTAAATTCCAACGACAGCCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACATGGCAGCGATGACGGAGAAAGGGAGGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGATTCAGTGTAAAGTACAAGATGTTGGACTCCATCTTGTATTGGATCGATGTCATGAGATACGATAAATTAATTCCTTTTCCTTAA

mRNA sequence

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACTTGTCGCCGTCATTTTCGGAGTCACCGGTTTGGTCGGAAAAGAGTTAGCAAAAGCCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATACTAATTTTCAATTCATTTCCTGCGATTTGCTCGACCGCTCTTCCGCTAACCGAAACCTCTCGCCGGTCCGCCACGTCACACATCTTTTCTGGGTCACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTCGACGTCATTCTCCCGGCGGCGCCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGCCGCCGTCGGAGGCGGCGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCTGGAGGTGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACACCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACATGGCAGCGATGACGGAGAAAGGGAGGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGATTCAGTGTAAAGTACAAGATGTTGGACTCCATCTTGTATTGGATCGATGTCATGAGATACGATAAATTAATTCCTTTTCCTTAA

Coding sequence (CDS)

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACTTGTCGCCGTCATTTTCGGAGTCACCGGTTTGGTCGGAAAAGAGTTAGCAAAAGCCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATACTAATTTTCAATTCATTTCCTGCGATTTGCTCGACCGCTCTTCCGCTAACCGAAACCTCTCGCCGGTCCGCCACGTCACACATCTTTTCTGGGTCACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTCGACGTCATTCTCCCGGCGGCGCCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGCCGCCGTCGGAGGCGGCGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCTGGAGGTGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACACCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACATGGCAGCGATGACGGAGAAAGGGAGGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGATTCAGTGTAAAGTACAAGATGTTGGACTCCATCTTGTATTGGATCGATGTCATGAGATACGATAAATTAATTCCTTTTCCTTAA

Protein sequence

MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP

Homology

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BLAST of Carg12426 vs. NCBI nr
Match: XP_022948773.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 800.4 bits (2066), Expect = 2.7e-228
Identity = 387/391 (98.98%), Postives = 390/391 (99.74%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTH+FWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHY AAMTEKGR+WAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

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BLAST of Carg12426 vs. NCBI nr
Match: XP_023525980.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 1.9e-218
Identity = 375/391 (95.91%), Postives = 380/391 (97.19%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AA ALRHVSLQTGIKHYVSLT    GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AASALRHVSLQTGIKHYVSLT----GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV  AAEDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV--AAEDEE 300

Query: 301 GMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHY AAM EKG+VWAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           DRLGFSVKYKMLDSILYWIDVMR+DKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385

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BLAST of Carg12426 vs. NCBI nr
Match: XP_022998181.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 764.2 bits (1972), Expect = 2.1e-217
Identity = 375/393 (95.42%), Postives = 379/393 (96.44%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAAETINAKLDGNGELVAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITDTN
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTHLFWVTW AQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAV-GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
           AAPALRHVSLQTGIKHYVSLTGAAV        AVYCEDSPRAESGNNFYYALEDLLRER
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVDXXXXXXXAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240
           LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC
Sbjct: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240

Query: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV-AAAAED 300
           INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFG+ AAAAED
Sbjct: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAED 300

Query: 301 EEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360
           E+GMFSPEFHY AAM EKG+VWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN
Sbjct: 301 EKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           KSDRLGFSVKYKMLDSILYWIDVMRYD LIPFP
Sbjct: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDTLIPFP 393

[Close]

BLAST of Carg12426 vs. NCBI nr
Match: XP_004145319.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN65803.1 hypothetical protein Csa_1G530150 [Cucumis sativus])

HSP 1 Score: 649.8 bits (1675), Expect = 5.8e-183
Identity = 321/395 (81.27%), Postives = 338/395 (85.57%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MA E IN  LD NGELVAVIFGVTGLVGK+LAK LLST GWKVYGVARRPDNVSPI+   
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR S  +NLSPVRHVTHLFW+TWAAQLR D+ DCSDQNR MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLT--GAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRE 180
           +APALRHVSLQTGIKHY SLT   A  GGGG    VYCEDSPRAESGNNFYY LEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGF 240
           RLS G R VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE  
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 CINGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVA--A 300
            I+GSDARL AEQH+W AT   EIN TADGEAFNVCNGWSF WKEIWGAVAEK GVA   
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AAEDEEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360
           A E++E MFS EFHY AAM +KG+VWAEIVEEEGL+ TEMEELANWGFLD+LFRLP KMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 390
           ASR KSDRLGF+VKYKMLDS+LYWIDVMR DKLIP
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIP 395

[Close]

BLAST of Carg12426 vs. NCBI nr
Match: XP_022964858.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 646.4 bits (1666), Expect = 6.4e-182
Identity = 318/393 (80.92%), Postives = 340/393 (86.51%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MA E++N  LD NGELVAVIFGVTGLVGKELAK LLST GWKVYGVARRPDN+SPI+D N
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR SA+ NLSP+ HVTH+FW+TWAAQLRFD+ DCSDQN+ MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           +APALRHVSLQTG+KHY SLT ++ G GGG + VY E+SPRAESGNNFYY LEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLT-SSTGSGGGEV-VYSENSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SG  R VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I
Sbjct: 181 SGRRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 NGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAED 300
           + SDARL AEQHVWAAT   EIN   DGEAFNVCNGWSFTWKEIWGAVAEK GV   AED
Sbjct: 241 DSSDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAED 300

Query: 301 EEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360
           E  MFSPEF Y AAM EKG VWAEIVEEEGL+ TEM ELANWGFLDSLFRLP KMVASR 
Sbjct: 301 ER-MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRA 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 392
           KSDRLGF+VKYKMLDSILYWIDVMR+DKLIP P
Sbjct: 361 KSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Carg12426 vs. TAIR10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 401.4 bits (1030), Expect = 6.5e-112
Identity = 206/378 (54.50%), Postives = 261/378 (69.05%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKALL-STGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSS 74
           E VA+IFGVTGLVG+E+ K LL S  GW++YGVAR P+ ++ +T   + FISCDLL+ S 
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPE-INSMTKM-YNFISCDLLNASE 75

Query: 75  ANRNLSPVRH-VTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 134
             + LSP++  V+H+FWVTW+ +   DT +C  QN+TML N LD ILP A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GIKHYVSLTGAAVG-GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSV 194
           G+KHYVSL    +  G G  +  Y E+ PR  SG NFYY LEDLL+E+++     V WSV
Sbjct: 136 GMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS--VVWSV 195

Query: 195 LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQ 254
            RPGL++G S+RT +NFMGSLCVYGA+CK L  PFVFGG+  CWE   I+GSD+ LVAEQ
Sbjct: 196 QRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQ 255

Query: 255 HVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYMA 314
           H++AAT+       GEAFN  NG  FTWKEIW  + +K GV     +E  MF   F +  
Sbjct: 256 HIFAATSG-KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQV---NETTMFDEGFWFGR 315

Query: 315 AMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKM 374
            M E+  VW EIV +E L+ TE+E+LANW FLD+LFR P K++  R K DR GF  KY+ 
Sbjct: 316 EMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRT 375

Query: 375 LDSILYWIDVMRYDKLIP 390
           LDS+LYWIDVMR +KLIP
Sbjct: 376 LDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of Carg12426 vs. TAIR10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 250.4 bits (638), Expect = 1.9e-66
Identity = 156/380 (41.05%), Postives = 204/380 (53.68%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 75  RSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSL 134
                  LSP+  VTH+F+VTW    R   S+  + N +ML NVL  I+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHYVSLTGAAVGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAW 194
           QTG KHY+   G      G R    + ED PR +   NFYY  ED+L E +      V W
Sbjct: 146 QTGTKHYL---GPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTW 205

Query: 195 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVA 254
           S+ RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+A
Sbjct: 206 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIA 265

Query: 255 EQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHY 314
           EQ +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     +E          
Sbjct: 266 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE----GKNLGL 325

Query: 315 MAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKY 374
           +  M  K RVW E+V+E  L   ++EE+  W F D +  + G M+ S NKS   GF    
Sbjct: 326 VEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFR 385

Query: 375 KMLDSILYWIDVMRYDKLIP 390
              +S + WID  +  K++P
Sbjct: 386 NSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of Carg12426 vs. Swiss-Prot
Match: sp|A0A1C9CX66|CYC2_CAMAC (Iridoid synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.0e-66
Identity = 159/378 (42.06%), Postives = 210/378 (55.56%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRS 76
           VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D   ++I CD+ D S
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISDAS 87

Query: 77  SANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 136
             +  LSP+  VTH+FWVTWA   R   S+C + N TML NVL+ ++P A  L H+ LQT
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147

Query: 137 GIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 196
           G KHY+     A G        + ED PR  +  NFYY LED+L E  S     + WSV 
Sbjct: 148 GHKHYIG-PFEAFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVE-ASEKKAGLNWSVH 207

Query: 197 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 256
           RP +I G S  +  N +G+LCVY AICK    P  F G+ A W  + +  SDA L+AE  
Sbjct: 208 RPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSV-ASDADLIAEHQ 267

Query: 257 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYMAA 316
           +WAA   ++  A  EAFN  NG  F WK +W  +AE+FGV  A  DE          +  
Sbjct: 268 IWAA---VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDES---EKPTSLVER 327

Query: 317 MTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGK-MVASRNKSDRLGFSVKYKM 376
           M +KG VW EIV E GL  T++E +A W F D +  L G+ ++ S NKS   G+      
Sbjct: 328 MKDKGPVWEEIVRENGLHTTKLEGVATWWFADVI--LGGECLLDSMNKSKEHGYLGFRNT 387

Query: 377 LDSILYWIDVMRYDKLIP 390
            +S++  ID M+  K++P
Sbjct: 388 KNSLISVIDKMKAHKIVP 390

[Close]

BLAST of Carg12426 vs. Swiss-Prot
Match: sp|Q9STX2|VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.4e-65
Identity = 156/380 (41.05%), Postives = 204/380 (53.68%), Query Frame = 0


Query: 15  ELVAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 75  RSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSL 134
                  LSP+  VTH+F+VTW    R   S+  + N +ML NVL  I+P AP LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHYVSLTGAAVGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAW 194
           QTG KHY+   G      G R    + ED PR +   NFYY  ED+L E +      V W
Sbjct: 146 QTGTKHYL---GPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTW 205

Query: 195 SVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVA 254
           S+ RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+A
Sbjct: 206 SIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIA 265

Query: 255 EQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHY 314
           EQ +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     +E          
Sbjct: 266 EQQIWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEE----GKNLGL 325

Query: 315 MAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKY 374
           +  M  K RVW E+V+E  L   ++EE+  W F D +  + G M+ S NKS   GF    
Sbjct: 326 VEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFR 385

Query: 375 KMLDSILYWIDVMRYDKLIP 390
              +S + WID  +  K++P
Sbjct: 386 NSNNSFISWIDKYKAFKIVP 388

[Close]

BLAST of Carg12426 vs. Swiss-Prot
Match: sp|Q6PQJ9|5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.7e-62
Identity = 151/377 (40.05%), Postives = 203/377 (53.85%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRS 76
           VA+I GVTG++G  LA+ L       G WKVYGVARR    +   D    ++ CD+ D  
Sbjct: 28  VALIVGVTGIIGNSLAEILPLADTPGGPWKVYGVARR-TRPAWHEDNPINYVQCDISDPD 87

Query: 77  SANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 136
            +   LSP+  VTH+F+VTWA   R    +  + N  M  NVLD ++P  P L+H+SLQT
Sbjct: 88  DSQAKLSPLTDVTHVFYVTWAN--RSTEQENCEANSKMFRNVLDAVIPNCPNLKHISLQT 147

Query: 137 GIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 196
           G KHY+     + G        Y ED PR +   NFYY LED++ E +      + WSV 
Sbjct: 148 GRKHYMG-PFESYGKIESHDPPYTEDLPRLKY-MNFYYDLEDIMLEEVE-KKEGLTWSVH 207

Query: 197 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 256
           RPG I G S  +  N +G+LCVY AICK   K   F G  A W+G+  + SDA L+AE H
Sbjct: 208 RPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAWDGYS-DCSDADLIAEHH 267

Query: 257 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYMAA 316
           +WAA   ++  A  EAFNV NG  F WK  W  +AE+FGV    E EEG+   +      
Sbjct: 268 IWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGV-GCGEYEEGV---DLKLQDL 327

Query: 317 MTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKML 376
           M  K  VW EIV E GL PT+++++  W F D +       + S NKS   GF       
Sbjct: 328 MKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFRNSK 387

Query: 377 DSILYWIDVMRYDKLIP 390
           ++ + WID  +  K++P
Sbjct: 388 NAFISWIDKAKAYKIVP 389

[Close]

BLAST of Carg12426 vs. Swiss-Prot
Match: sp|A0A1C9CX56|CYC1_CAMAC (Iridoid synthase OS=Camptotheca acuminata OX=16922 GN=CYC1 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.2e-59
Identity = 149/384 (38.80%), Postives = 204/384 (53.12%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRS 76
           V +I GVTG+VG  LA+ L       G WKVYGVARRP   +  TD   ++I CD+    
Sbjct: 27  VGLIIGVTGIVGNSLAEILPLPDTPGGPWKVYGVARRP-RPAWNTDHPIEYIQCDVASTE 86

Query: 77  SANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 136
                LSP+ ++TH+F+V W       + DC + N TM  NVL  ++P AP LRH+ +QT
Sbjct: 87  DTLAKLSPLTNITHIFYVAWTG-----SEDC-EVNETMFHNVLKAVIPNAPNLRHICIQT 146

Query: 137 GIKHYVSLTGAAVGGGG--GRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWS 196
           GIKHY   TG     GG     + + ED PR  +  NFY+ LED+L E  +   + + WS
Sbjct: 147 GIKHY---TGPLDPDGGIQPHDSPFTEDLPRL-NAPNFYHNLEDILIEE-AAKKKGLTWS 206

Query: 197 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGG----SAACWEGFCINGSDAR 256
           V RP L+ G S  +  N +G+L VY  ICK   KP ++ G    S   W  +  + +DA 
Sbjct: 207 VHRPALVFGFSPYSMMNIIGALSVYAVICKHENKPLIYHGAPDNSRDSWNVYA-DAADAD 266

Query: 257 LVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAA-EDEEGMFSP 316
           L+AE  +WAA   +++ A  E FN  NG  F WK++W  +AE+F V     ED +   S 
Sbjct: 267 LIAEHQIWAA---VDSNAKNEVFNCSNGDVFKWKQMWKILAEQFEVELVGYEDGQKRMS- 326

Query: 317 EFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGF 376
                  M  KG VW EIV +  L+PT++E++A W F D +      +V   NKS   GF
Sbjct: 327 ---LQERMKGKGPVWDEIVRKHKLLPTKLEDVAQWWFAD-IVSQSQNLVNGVNKSKENGF 386

Query: 377 SVKYKMLDSILYWIDVMRYDKLIP 390
                   S +YWI  MR  K+IP
Sbjct: 387 LGFRDSKKSFVYWIKKMRAAKIIP 389

[Close]

BLAST of Carg12426 vs. Swiss-Prot
Match: sp|K7WDL7|IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.2e-58
Identity = 145/381 (38.06%), Postives = 205/381 (53.81%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKAL----LSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRS 76
           VA++ GVTG+VG  LA+ L       G WKVYGVARRP  V  +     ++I CD+ D  
Sbjct: 31  VALVVGVTGIVGSSLAEVLKLPDTPGGPWKVYGVARRPCPVW-LAKKPVEYIQCDVSDNQ 90

Query: 77  SANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQT 136
                LSP++ +TH+F+V+W       + DC   N TM  N+L+ ++P A  L+HV LQT
Sbjct: 91  ETISKLSPLKDITHIFYVSW-----IGSEDC-QTNATMFKNILNSVIPNASNLQHVCLQT 150

Query: 137 GIKHYVSL--TGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWS 196
           GIKHY  +   G+ V       + + ED PR  +  NFY+ LED+L E    G   + WS
Sbjct: 151 GIKHYFGIFEEGSKV---VPHDSPFTEDLPRL-NVPNFYHDLEDILYEET--GKNNLTWS 210

Query: 197 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAE 256
           V RP L+ G S  +  N + +LCVY  ICK   K  V+ GS   W  +  +  DA LVAE
Sbjct: 211 VHRPALVFGFSPCSMMNIVSTLCVYATICKHENKALVYPGSKNSWNCYA-DAVDADLVAE 270

Query: 257 QHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV--AAAAEDEEGMFSPEFH 316
             +WAA   ++  A  +  N  NG  F WK IW  +AE+FG+      E +E +   E  
Sbjct: 271 HEIWAA---VDPKAKNQVLNCNNGDVFKWKHIWKKLAEEFGIEMVGYVEGKEQVSLAEL- 330

Query: 317 YMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVK 376
               M +K +VW EIV++  L+PT+++E+A + F D  F     +++S NKS  LGF   
Sbjct: 331 ----MKDKDQVWDEIVKKNNLVPTKLKEIAAFWFADIAF-CSENLISSMNKSKELGFLGF 388

Query: 377 YKMLDSILYWIDVMRYDKLIP 390
              + S +  ID MR  + IP
Sbjct: 391 RNSMKSFVSCIDKMRDYRFIP 388

[Close]

BLAST of Carg12426 vs. TrEMBL
Match: tr|A0A0A0LVI2|A0A0A0LVI2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 3.8e-183
Identity = 321/395 (81.27%), Postives = 338/395 (85.57%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MA E IN  LD NGELVAVIFGVTGLVGK+LAK LLST GWKVYGVARRPDNVSPI+   
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR S  +NLSPVRHVTHLFW+TWAAQLR D+ DCSDQNR MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLT--GAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRE 180
           +APALRHVSLQTGIKHY SLT   A  GGGG    VYCEDSPRAESGNNFYY LEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGF 240
           RLS G R VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE  
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 CINGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVA--A 300
            I+GSDARL AEQH+W AT   EIN TADGEAFNVCNGWSF WKEIWGAVAEK GVA   
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 AAEDEEGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMV 360
           A E++E MFS EFHY AAM +KG+VWAEIVEEEGL+ TEMEELANWGFLD+LFRLP KMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 390
           ASR KSDRLGF+VKYKMLDS+LYWIDVMR DKLIP
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIP 395

[Close]

BLAST of Carg12426 vs. TrEMBL
Match: tr|A0A1S3C4Y3|A0A1S3C4Y3_CUCME (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=4 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 1.4e-180
Identity = 316/392 (80.61%), Postives = 333/392 (84.95%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAA+ IN  LD NGELVAVIFGVTGLVGK+LAK LLST GWKVYGVARRPDNVSPI+   
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR S  +NLSPVRHVTHLFW+TWAAQLR D+  CSDQNR MLAN LD ILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           +APALRHVSLQTG+KHY SLT  A G GG    VYCEDSPRAESGNNFYY LEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           S G R VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I
Sbjct: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 NGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAED 300
           +GSDARL AEQH+W AT   EIN TADGEAFNVCNGWSF WKEIWG VAEK GVA  AE+
Sbjct: 241 DGSDARLTAEQHMWVATKAVEINATADGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEE 300

Query: 301 E-EGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASR 360
           E E MFS EFHY AAM +KG+VWAEIVEEEGL+ TEMEELANW FLD+LFRLP KMVASR
Sbjct: 301 EKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASR 360

Query: 361 NKSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 390
            KSDR GF+VKYKMLDSILYWIDVMR DKLIP
Sbjct: 361 AKSDRFGFNVKYKMLDSILYWIDVMRNDKLIP 392

[Close]

BLAST of Carg12426 vs. TrEMBL
Match: tr|A0A1S3C5H7|A0A1S3C5H7_CUCME (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103497085 PE=4 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 4.9e-178
Identity = 311/390 (79.74%), Postives = 329/390 (84.36%), Query Frame = 0


Query: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60
           MAA+ IN  LD NGELVAVIFGVTGLVGK+LAK LLST GWKVYGVARRPDNVSPI+   
Sbjct: 1   MAAQIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR S  +NLSPVRHVTHLFW+TWAAQLR D+  CSDQNR MLAN LD ILP
Sbjct: 61  FHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAAQLRLDSPGCSDQNRAMLANALDAILP 120

Query: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           +APALRHVSLQTG+KHY SLT  A G GG    VYCEDSPRAESGNNFYY LEDLLRERL
Sbjct: 121 SAPALRHVSLQTGVKHYASLTRLAAGCGGREEVVYCEDSPRAESGNNFYYVLEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           S G R VAWSVLRPGLILG SNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I
Sbjct: 181 SCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDE- 300
           +GSDARL AEQH+W AT  +    DGEAFNVCNGWSF WKEIWG VAEK GVA  AE+E 
Sbjct: 241 DGSDARLTAEQHMWVATKAVEIN-DGEAFNVCNGWSFRWKEIWGTVAEKLGVATTAEEEK 300

Query: 301 EGMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNK 360
           E MFS EFHY AAM +KG+VWAEIVEEEGL+ TEMEELANW FLD+LFRLP KMVASR K
Sbjct: 301 ERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEMEELANWVFLDTLFRLPEKMVASRAK 360

Query: 361 SDRLGFSVKYKMLDSILYWIDVMRYDKLIP 390
           SDR GF+VKYKMLDSILYWIDVMR DKLIP
Sbjct: 361 SDRFGFNVKYKMLDSILYWIDVMRNDKLIP 389

[Close]

BLAST of Carg12426 vs. TrEMBL
Match: tr|A0A1U8AXK6|A0A1U8AXK6_NELNU (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Nelumbo nucifera OX=4432 GN=LOC104608495 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 6.9e-124
Identity = 224/385 (58.18%), Postives = 283/385 (73.51%), Query Frame = 0


Query: 5   TINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFI 64
           TI+ K D   E VA+IFGVTGLVGKE+A  LL+  GWKVYG+AR+PD  +PI   N++FI
Sbjct: 11  TISTKKD-VAESVALIFGVTGLVGKEIANNLLAKSGWKVYGIARKPDFFTPIQHRNYRFI 70

Query: 65  SCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPA 124
           SCDLLD     + LS +   TH+FWVTWA+Q   D+S+C DQN+ M++N LD ILP A A
Sbjct: 71  SCDLLDPVQTVQKLSSLNGATHIFWVTWASQFPLDSSECCDQNKAMMSNALDAILPKAKA 130

Query: 125 LRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGG 184
           L+HVSLQTG KHYVSL+G         +  Y E+SPR   G+NFYY LEDLL E+L G  
Sbjct: 131 LKHVSLQTGTKHYVSLSGPF----DNTVRRYDEESPRVNEGHNFYYMLEDLLIEKLRG-- 190

Query: 185 RRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSD 244
            +V+WSV RPGLILG S+RT FNF+GSLC+YG ICK L  PFVFGG+  CWE   I+GSD
Sbjct: 191 -KVSWSVHRPGLILGSSHRTAFNFIGSLCIYGTICKHLHLPFVFGGARECWEEVYIDGSD 250

Query: 245 ARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFS 304
           ARLVAEQH+W +TN+  ++ DG+AFN  NG SFTWK+IW A+A KFG+ AA    E MFS
Sbjct: 251 ARLVAEQHIWVSTNDNVSSTDGQAFNAVNGPSFTWKQIWPALAVKFGLQAA----EDMFS 310

Query: 305 PEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLG 364
            +F +  +M +K  VW +IV +EGL PT++ +LANW FLD LFR P K++A+R+K DRLG
Sbjct: 311 QDFLFSTSMADKESVWEDIVVKEGLRPTKIGDLANWTFLDVLFRFPTKLLAARDKVDRLG 370

Query: 365 FSVKYKMLDSILYWIDVMRYDKLIP 390
           F++ Y+MLDSILYW+D +R +KLIP
Sbjct: 371 FTMTYEMLDSILYWVDCIRDEKLIP 383

[Close]

BLAST of Carg12426 vs. TrEMBL
Match: tr|A0A1U8AX67|A0A1U8AX67_NELNU (3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Nelumbo nucifera OX=4432 GN=LOC104608495 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 6.9e-124
Identity = 224/385 (58.18%), Postives = 283/385 (73.51%), Query Frame = 0


Query: 5   TINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFI 64
           TI+ K D   E VA+IFGVTGLVGKE+A  LL+  GWKVYG+AR+PD  +PI   N++FI
Sbjct: 14  TISTKKD-VAESVALIFGVTGLVGKEIANNLLAKSGWKVYGIARKPDFFTPIQHRNYRFI 73

Query: 65  SCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPA 124
           SCDLLD     + LS +   TH+FWVTWA+Q   D+S+C DQN+ M++N LD ILP A A
Sbjct: 74  SCDLLDPVQTVQKLSSLNGATHIFWVTWASQFPLDSSECCDQNKAMMSNALDAILPKAKA 133

Query: 125 LRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGG 184
           L+HVSLQTG KHYVSL+G         +  Y E+SPR   G+NFYY LEDLL E+L G  
Sbjct: 134 LKHVSLQTGTKHYVSLSGPF----DNTVRRYDEESPRVNEGHNFYYMLEDLLIEKLRG-- 193

Query: 185 RRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSD 244
            +V+WSV RPGLILG S+RT FNF+GSLC+YG ICK L  PFVFGG+  CWE   I+GSD
Sbjct: 194 -KVSWSVHRPGLILGSSHRTAFNFIGSLCIYGTICKHLHLPFVFGGARECWEEVYIDGSD 253

Query: 245 ARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFS 304
           ARLVAEQH+W +TN+  ++ DG+AFN  NG SFTWK+IW A+A KFG+ AA    E MFS
Sbjct: 254 ARLVAEQHIWVSTNDNVSSTDGQAFNAVNGPSFTWKQIWPALAVKFGLQAA----EDMFS 313

Query: 305 PEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLG 364
            +F +  +M +K  VW +IV +EGL PT++ +LANW FLD LFR P K++A+R+K DRLG
Sbjct: 314 QDFLFSTSMADKESVWEDIVVKEGLRPTKIGDLANWTFLDVLFRFPTKLLAARDKVDRLG 373

Query: 365 FSVKYKMLDSILYWIDVMRYDKLIP 390
           F++ Y+MLDSILYW+D +R +KLIP
Sbjct: 374 FTMTYEMLDSILYWVDCIRDEKLIP 386

The following BLAST results are available for this feature:

BLAST of Carg12426 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948773.1	2.7e-228	98.98	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]
XP_023525980.1	1.9e-218	95.91	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022998181.1	2.1e-217	95.42	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]
XP_004145319.1	5.8e-183	81.27	PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN65803...	[more]
XP_022964858.1	6.4e-182	80.92	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]

BLAST of Carg12426 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G58750.1	6.5e-112	54.50	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	1.9e-66	41.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]

BLAST of Carg12426 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|A0A1C9CX66\|CYC2_CAMAC	3.0e-66	42.06	Iridoid synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1	[more]
sp\|Q9STX2\|VEP1_ARATH	3.4e-65	41.05	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
sp\|Q6PQJ9\|5BPOR_DIGLA	2.7e-62	40.05	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]
sp\|A0A1C9CX56\|CYC1_CAMAC	1.2e-59	38.80	Iridoid synthase OS=Camptotheca acuminata OX=16922 GN=CYC1 PE=1 SV=1	[more]
sp\|K7WDL7\|IRIS_CATRO	5.2e-58	38.06	Iridoid synthase OS=Catharanthus roseus OX=4058 PE=1 SV=1	[more]

BLAST of Carg12426 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LVI2\|A0A0A0LVI2_CUCSA	3.8e-183	81.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=4 SV=1	[more]
tr\|A0A1S3C4Y3\|A0A1S3C4Y3_CUCME	1.4e-180	80.61	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Cucumis melo OX=3656 GN=...	[more]
tr\|A0A1S3C5H7\|A0A1S3C5H7_CUCME	4.9e-178	79.74	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Cucumis melo OX=3656 GN=...	[more]
tr\|A0A1U8AXK6\|A0A1U8AXK6_NELNU	6.9e-124	58.18	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 OS=Nelumbo nucifera OX=4432...	[more]
tr\|A0A1U8AX67\|A0A1U8AX67_NELNU	6.9e-124	58.18	3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 OS=Nelumbo nucifera OX=4432...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0050662	coenzyme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR036291	NAD(P)-bd_dom_sf
IPR001509	Epimerase_deHydtase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0050662	coenzyme binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12426-RA	Carg12426-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 19..134 e-value: 2.5E-8 score: 33.6
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 17..389 e-value: 1.9E-93 score: 315.4
None	No IPR available	PANTHER	PTHR32487	FAMILY NOT NAMED	coord: 7..389
None	No IPR available	PANTHER	PTHR32487:SF7	SUBFAMILY NOT NAMED	coord: 7..389
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 17..328 e-value: 1.98375E-96 score: 292.227
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 17..294

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg12426	Cla008168	Watermelon (97103) v1	carwmB0440
Carg12426	Csa1G530150	Cucumber (Chinese Long) v2	carcuB0471
Carg12426	MELO3C020553	Melon (DHL92) v3.5.1	carmeB0449
Carg12426	ClCG03G000790	Watermelon (Charleston Gray)	carwcgB0436
Carg12426	CSPI01G23840	Wild cucumber (PI 183967)	carcpiB0476
Carg12426	Cucsa.147030	Cucumber (Gy14) v1	carcgyB0509
Carg12426	CmaCh01G006600	Cucurbita maxima (Rimu)	carcmaB0613
Carg12426	CmaCh13G000690	Cucurbita maxima (Rimu)	carcmaB0610
Carg12426	CmoCh01G006910	Cucurbita moschata (Rifu)	carcmoB0596
Carg12426	CmoCh13G000580	Cucurbita moschata (Rifu)	carcmoB0594
Carg12426	Lsi02G012870	Bottle gourd (USVL1VR-Ls)	carlsiB403
Carg12426	Cp4.1LG20g08120	Cucurbita pepo (Zucchini)	carcpeB0620
Carg12426	Cp4.1LG02g11730	Cucurbita pepo (Zucchini)	carcpeB0621
Carg12426	CsGy1G023320	Cucumber (Gy14) v2	carcgybB0448
Carg12426	MELO3C020553.2	Melon (DHL92) v3.6.1	carmedB0451
Carg12426	CsaV3_1G036150	Cucumber (Chinese Long) v3	carcucB0472
Carg12426	Cla97C03G051600	Watermelon (97103) v2	carwmbB0467
Carg12426	Bhi08G000424	Wax gourd	carwgoB0535

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg12426	Carg20604	Silver-seed gourd	carcarB408

The following block(s) are covering this gene:


Gene	Organism	Block
Carg12426	Silver-seed gourd	carcarB192
Carg12426	Cucurbita pepo (Zucchini)	carcpeB0619
Carg12426	Melon (DHL92) v3.5.1	carmeB0450
Carg12426	Melon (DHL92) v3.6.1	carmedB0453
Carg12426	Watermelon (97103) v1	carwmB0438
Carg12426	Watermelon (97103) v2	carwmbB0463