Cp4.1LG20g08120 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG20g08120
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionProgesterone 5-beta-reductase, putative
LocationCp4.1LG20 : 7795144 .. 7796404 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGCCAATGGCGAACTGGTAGCTGTCATTTTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATCTCTTGCAATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCCTTCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGACTCCCAGGATTGCTCCGACCAGAACAAGGCCATGTTGGCGAACGCCCTCGATGCTATTCTCCCGTCGGCGCTGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCGGCGGCGGAGGAGGGGAGGTGGTTTATTGTGAGGACAGCCCGAGAGCGGAGTCCGTGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCCATTTGCAAGAAGTTGAACATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGACGGCTCCGACGCTCGGCTCACGGCGGAGCAGCACGTGTGGGCCGCAACAAAAGCCGTTGAAATAAATGGTATGTAACACCTACATCGAGCTGTCATAAATATAAATATATATTTTTTTTTTTTATAAGAGACAATTTCATTCATGATTGATAAATATAAATTTAATATCTGTTAACATGTAAAAATACCGTAATCTCAGATGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAAGAGATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGCGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTTCATTATACGGCAGCTATGGCGGAGAAAGGGACGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGAAAATGGGGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAGTCCGACCGGTTAGGGTTCACTGTTAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCT

mRNA sequence

ACGCCAATGGCGAACTGGTAGCTGTCATTTTCGGAGTCACCGGGTTGGTCGGAAAAGAGTTGGCCAAAATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATCTCTTGCAATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCCTTCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGACTCCCAGGATTGCTCCGACCAGAACAAGGCCATGTTGGCGAACGCCCTCGATGCTATTCTCCCGTCGGCGCTGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCGGCGGCGGAGGAGGGGAGGTGGTTTATTGTGAGGACAGCCCGAGAGCGGAGTCCGTGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCCATTTGCAAGAAGTTGAACATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGACGGCTCCGACGCTCGGCTCACGGCGGAGCAGCACGTGTGGGCCGCAACAAAAGCCATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGCGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTTCATTATACGGCAGCTATGGCGGAGAAAGGGACGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGAAAATGGGGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAGTCCGACCGGTTAGGGTTCACTGTTAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCT

Coding sequence (CDS)

ATGCTTCTCTCAACCGCTGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGATAATATCAGCCCCATCTCCGATCCCAACTTCCACTTCATCTCTTGCAATTTGCTCGACCGCCGTTCGGCTCACCTGAACCTCTCACCCCTTCGCCACGTCACCCACATGTTCTGGATCACTTGGGCCGCCCAGCTCCGGTTCGACTCCCAGGATTGCTCCGACCAGAACAAGGCCATGTTGGCGAACGCCCTCGATGCTATTCTCCCGTCGGCGCTGGCCCTCCGCCACGTCTCTCTGCAGACCGGGGTCAAGCATTACGCTTCTCTCACGAGCTCCACCGGCGGCGGAGGAGGGGAGGTGGTTTATTGTGAGGACAGCCCGAGAGCGGAGTCCGTGAATAATTTTTACTATGTGTTGGAGGATCTCCTCCGCGAGCGGCTGTCCGGCTACCGGAGAGTGGTGGCGTGGAGTATTCTCCGACCGGGTTTGATACTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGCGCCATTTGCAAGAAGTTGAACATGCCATTTGTGTTCGGCGGCACGGCGGCGTGCTGGGAGGAGGTTTATATCGACGGCTCCGACGCTCGGCTCACGGCGGAGCAGCACGTGTGGGCCGCAACAAAAGCCATATGGGGGGCGGTGGCGGAGAAGCTTGGCGTGACGGCGGGGGCGGAGGACGAGAGGATGTTTTCGCCGGAGTTTCATTATACGGCAGCTATGGCGGAGAAAGGGACGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGGTTGCGACGAAAATGGGGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCGAGTCGAGCCAAGTCCGACCGGTTAGGGTTCACTGTTAAATACAAGATGTTGGACTCCATCTTGTATTGGATTGATGTCATGAGACACGACAAATTGATTCCGTCTCCT

Protein sequence

MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPSP
Homology
[Close]
BLAST of Cp4.1LG20g08120 vs. Swiss-Prot
Match: VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.5e-50
Identity = 125/343 (36.44%), Postives = 172/343 (50.15%), Query Frame = 1 

Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP        P   +I C++ D       LSPL  VTH+F++TW  +   +S
Sbjct: 56  WKVYGVARRPRPTWNADHP-IDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNR-ESES 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA 127
           ++C + N +ML N L AI+P A  LRHV LQTG KHY    ++  G   +  + ED PR 
Sbjct: 116 ENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRL 175

Query: 128 ESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL 187
           + + NFYY  ED+L E +      V WSI RP +I G S  +  N +G+LCVY AICK  
Sbjct: 176 Q-IQNFYYTQEDILFEEIKKI-ETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHE 235

Query: 188 NMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWGA 247
             P +F G+   WE  ++  SDA L AEQ +WAA                    K +W  
Sbjct: 236 GSPLLFPGSKKAWEG-FMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKI 295

Query: 248 VAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSL 307
           +AE+ G+     +E            M  K  VW E+V+E  L   K+ E+  W F D +
Sbjct: 296 LAEQFGIEEYGFEE---GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVI 355

Query: 308 FRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
             + E M+ S  KS   GF       +S + WID  +  K++P
Sbjct: 356 LGV-EGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cp4.1LG20g08120 vs. Swiss-Prot
Match: 5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 125/344 (36.34%), Postives = 172/344 (50.00%), Query Frame = 1 

Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARR        +P  +++ C++ D   +   LSPL  VTH+F++TWA   R   
Sbjct: 56  WKVYGVARRTRPAWHEDNP-INYVQCDISDPDDSQAKLSPLTDVTHVFYVTWAN--RSTE 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGG-GGGEVVYCEDSPR 127
           Q+  + N  M  N LDA++P+   L+H+SLQTG KHY     S G     +  Y ED PR
Sbjct: 116 QENCEANSKMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESYGKIESHDPPYTEDLPR 175

Query: 128 AESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKK 187
            + + NFYY LED++ E +   +  + WS+ RPG I G S  +  N +G+LCVY AICK 
Sbjct: 176 LKYM-NFYYDLEDIMLEEVE-KKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKH 235

Query: 188 LNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWG 247
                 F G  A W+  Y D SDA L AE H+WAA                    K  W 
Sbjct: 236 EGKVLRFTGCKAAWDG-YSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWK 295

Query: 248 AVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDS 307
            +AE+ GV  G  +E +   +      M  K  VW EIV E GL  TK+ ++  W F D 
Sbjct: 296 VLAEQFGVGCGEYEEGV---DLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDV 355

Query: 308 LFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           +    E  + S  KS   GF       ++ + WID  +  K++P
Sbjct: 356 ILG-NECFLDSMNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]
BLAST of Cp4.1LG20g08120 vs. Swiss-Prot
Match: IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.5e-47
Identity = 123/348 (35.34%), Postives = 174/348 (50.00%), Query Frame = 1 

Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP  +     P   +I C++ D +     LSPL+ +TH+F+++W       S
Sbjct: 59  WKVYGVARRPCPVWLAKKP-VEYIQCDVSDNQETISKLSPLKDITHIFYVSWIG-----S 118

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVV-----YCE 127
           +DC   N  M  N L++++P+A  L+HV LQTG+KHY  +       G +VV     + E
Sbjct: 119 EDCQT-NATMFKNILNSVIPNASNLQHVCLQTGIKHYFGIFEE----GSKVVPHDSPFTE 178

Query: 128 DSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGA 187
           D PR  +V NFY+ LED+L E     +  + WS+ RP L+ G S  +  N + +LCVY  
Sbjct: 179 DLPRL-NVPNFYHDLEDILYEETG--KNNLTWSVHRPALVFGFSPCSMMNIVSTLCVYAT 238

Query: 188 ICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------K 247
           ICK  N   V+ G+   W   Y D  DA L AE  +WAA                    K
Sbjct: 239 ICKHENKALVYPGSKNSW-NCYADAVDADLVAEHEIWAAVDPKAKNQVLNCNNGDVFKWK 298

Query: 248 AIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWG 307
            IW  +AE+ G+      E     +      M +K  VW EIV++  LV TK+ E+A + 
Sbjct: 299 HIWKKLAEEFGIEMVGYVEG--KEQVSLAELMKDKDQVWDEIVKKNNLVPTKLKEIAAFW 358

Query: 308 FLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           F D  F   E +++S  KS  LGF      + S +  ID MR  + IP
Sbjct: 359 FADIAF-CSENLISSMNKSKELGFLGFRNSMKSFVSCIDKMRDYRFIP 388

[Close]
BLAST of Cp4.1LG20g08120 vs. Swiss-Prot
Match: YJ72_SCHPO (Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC757.02c PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.7e-07
Identity = 50/159 (31.45%), Postives = 75/159 (47.17%), Query Frame = 1 

Query: 74  NKAMLANALDAI-LPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA----E 133
           N  ML N + A+ L S   LR V L TG+K Y         G   +   E   R      
Sbjct: 97  NGNMLRNFVQALELTSIQTLRRVILTTGLKFYGLHL-----GEVRLPMIETDIRVPETFS 156

Query: 134 SVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLN 193
              NFYYV ED+L+E  +G  +   ++I  P  I G S  ++ N   ++ +Y  +C++L+
Sbjct: 157 GTPNFYYVQEDILKEFSNG--KKWDYTIAMPNDICGVSKGSYMNEAFTIALYALVCRELH 216

Query: 194 MPFVFGGTAACWEEVYI---DGSDARLTAEQHVWAATKA 225
            PF F G     E+ Y+   D S ++L A+  +W   KA
Sbjct: 217 EPFRFPGN----EKFYLGFDDISYSKLIADFQLWMTFKA 244

[Close]
BLAST of Cp4.1LG20g08120 vs. TrEMBL
Match: A0A0A0LVI2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G530150 PE=4 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 2.9e-162
Identity = 292/362 (80.66%), Postives = 307/362 (84.81%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDRRS   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSST----GGGGGE 121
           QLR DS DCSDQN+AMLANALDAILPSA ALRHVSLQTG+KHYASLT       GGG GE
Sbjct: 95  QLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGE 154

Query: 122 VVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSL 181
           VVYCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILGCSNRTFFNFMGSL
Sbjct: 155 VVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSL 214

Query: 182 CVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA------------- 241
           CVYGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA             
Sbjct: 215 CVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHIWVATKAVEINATADGEAFN 274

Query: 242 -----------IWGAVAEKLGV-TAGAEDE--RMFSPEFHYTAAMAEKGTVWAEIVEEEG 301
                      IWGAVAEKLGV T GAE+E  RMFS EFHYTAAM +KG VWAEIVEEEG
Sbjct: 275 VCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEG 334

Query: 302 LVATKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLI 333
           LV T+M ELANWGFLD+LFRLPEKMVASRAKSDRLGF VKYKMLDS+LYWIDVMR+DKLI
Sbjct: 335 LVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLI 394

[Close]
BLAST of Cp4.1LG20g08120 vs. TrEMBL
Match: A0A022RYY0_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a018609mg PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 8.2e-104
Identity = 195/356 (54.78%), Postives = 237/356 (66.57%), Query Frame = 1 

Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSP-LRHVTHMFWITW 60
           MLLST  WKVYG++RRP+ + P     + FI C+LL+      NLSP L  VTH+FW+TW
Sbjct: 28  MLLSTCDWKVYGISRRPEKVGPADVERYRFIPCDLLNPLETRKNLSPILDDVTHIFWVTW 87

Query: 61  AAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVV 120
           A+Q   DS DC +QN+AM+AN L+A+LP A ALRHVSLQTG KHY SLT S+    G   
Sbjct: 88  ASQFSLDSSDCYEQNRAMMANVLNALLPKAKALRHVSLQTGTKHYVSLTGSSDPQVGH-- 147

Query: 121 YCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCV 180
           Y E  PRA   +NFYY LEDLL+ERL  +   VAWSI RPGLI+GCS++T +NF+GS+CV
Sbjct: 148 YDEKCPRACLGHNFYYALEDLLQERLPSHN--VAWSIHRPGLIMGCSDKTLYNFIGSICV 207

Query: 181 YGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT----------------- 240
           YGAICK LN+PFVFGGT  CWEE+Y+D SDARL AEQH+W  T                 
Sbjct: 208 YGAICKHLNLPFVFGGTKECWEEMYLDASDARLVAEQHIWVGTNDKITSDNLQGQAFNAI 267

Query: 241 -------KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATK 300
                  K IW A+  K GV    E+   FS +F + + M +KG VW EIV +EGLV T 
Sbjct: 268 NGESYTWKEIWPAIGAKFGVNVH-ENVPFFSGDFVFASEMGDKGGVWKEIVAKEGLVDTN 327

Query: 301 MGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           + +LANW FLD LFR P K++ +R K D +GF  KYK LDSILYWIDVMR DK IP
Sbjct: 328 LMDLANWDFLDLLFRCPLKILGTRFKIDSMGFETKYKTLDSILYWIDVMRADKFIP 378

[Close]
BLAST of Cp4.1LG20g08120 vs. TrEMBL
Match: A0A0K9S2H9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_002350 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.5e-102
Identity = 195/357 (54.62%), Postives = 241/357 (67.51%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDP--NFHFISCNLLDRRSAHLNLSPLRHVTHMFWITW 61
           L S   WKVYG+AR+ + ++   +   N+HFISC+LL        LS L  VTH+FW+TW
Sbjct: 36  LSSKYSWKVYGIARKEEQLNSYRNTSNNYHFISCDLLCPFDTEKKLSCLEDVTHIFWVTW 95

Query: 62  AAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEV- 121
           A Q + DS +C +QNK ML+NALDA+LP A +L+HVSLQTG+KHY SL         +V 
Sbjct: 96  ANQFQLDSPECCEQNKEMLSNALDAMLPKAKSLKHVSLQTGMKHYVSLRGPISNNLRDVD 155

Query: 122 --VYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGS 181
             VY ED PRA   NNFYYVLEDL++ERLSG  +V AWS+ RPGLI GCSNRT +NFMG 
Sbjct: 156 IAVYDEDCPRASVGNNFYYVLEDLIQERLSGNNKV-AWSVQRPGLITGCSNRTLYNFMGC 215

Query: 182 LCVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------- 241
           L +YG+ICK LN+PFVFGG+  CWEE  ID SDARL AEQH+WA+T              
Sbjct: 216 LSIYGSICKHLNLPFVFGGSKECWEEAVIDVSDARLVAEQHIWASTDEQLSSNGGQPFNA 275

Query: 242 --------KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVAT 301
                   K IW A+ +K G+      E MFS EF Y+ AMA+KG VW EIVE++GLV T
Sbjct: 276 INGVCFTWKEIWPAIGKKFGLEV---PENMFSDEFMYSKAMADKGGVWREIVEKKGLVET 335

Query: 302 KMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           +M ELANW FLD LFR P K++ +RAK+DRLGF  +Y   +SIL+WID MR +KLIP
Sbjct: 336 EMEELANWPFLDILFRCPMKLLGTRAKADRLGFRKRYSASESILFWIDNMRDEKLIP 388

[Close]
BLAST of Cp4.1LG20g08120 vs. TrEMBL
Match: A0A103XNN3_CYNCS (NAD(P)-binding domain-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_003863 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.8e-102
Identity = 195/364 (53.57%), Postives = 243/364 (66.76%), Query Frame = 1 

Query: 1   MLLSTAGWKVYGVARRPDNISPI-----SDPNFHFISCNLLDRRSAHLNLSPLRHVTHMF 60
           +L S + WKVYGVARRP+ ++ I     ++PN+HFISCNLLD     + LS L  VTH+F
Sbjct: 30  LLSSRSKWKVYGVARRPEMVTMIKKPIHTNPNYHFISCNLLDPFDTQMKLSGLHDVTHVF 89

Query: 61  WITWAAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGG 120
           W+TWA+  + D+ +C DQNKAM++NALD+ILP A  L H SLQTG KHY SL   T    
Sbjct: 90  WVTWASHFQLDTIECYDQNKAMMSNALDSILPVAKGLSHFSLQTGTKHYVSLHGGTASFD 149

Query: 121 GEV----VYCEDSPRAESV--NNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRT 180
             V     Y E+ PR E+   +NFYY LEDLL+ERL G   VV WS+ RPGLI+G S +T
Sbjct: 150 HLVKKVCYYDENCPRVETQVGSNFYYALEDLLKERLGG---VVPWSVHRPGLIMGSSRKT 209

Query: 181 FFNFMGSLCVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT------- 240
            +NFMGSLCVYG+ICK LN+PFVFGG   CWEE ++D SDARL A+Q +WAAT       
Sbjct: 210 MYNFMGSLCVYGSICKYLNLPFVFGGRKECWEEQFVDASDARLVADQQIWAATNNLVQSK 269

Query: 241 ---------------KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVE 300
                          K IWGA+ EK G     E   M S +F ++  M++K  VW EIV 
Sbjct: 270 NGQAFNSINGDSSTWKDIWGAIGEKFGAVVPWE---MLSKDFTFSGFMSDKAGVWKEIVR 329

Query: 301 EEGLVATKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHD 332
           +EGLV T+M +LANW FLD+LFR P KM+ +RAK+DRLGFT +Y  LDSIL+WIDVMR +
Sbjct: 330 KEGLVDTEMDDLANWYFLDALFRCPVKMLGTRAKADRLGFTTRYTALDSILHWIDVMRKE 387

[Close]
BLAST of Cp4.1LG20g08120 vs. TrEMBL
Match: M5W676_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021134mg PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 7.6e-102
Identity = 193/354 (54.52%), Postives = 241/354 (68.08%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           L+S + WKVYG+AR P  IS     NFHFI C+LL+       LS L+ VTH+FW+TWA+
Sbjct: 35  LISKSKWKVYGIARNPMIISNQQCSNFHFIQCDLLNPLETQQRLSLLQDVTHVFWVTWAS 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYC 121
           Q + DS +C +QNKAM++N L+AI+P+A AL+HVSLQTG+KHY SL            Y 
Sbjct: 95  QYQLDSIECCEQNKAMMSNVLNAIVPTAKALKHVSLQTGMKHYVSLQGPFDEAEARY-YN 154

Query: 122 EDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYG 181
           E+ PR ++  NFYYVLEDLL+ERL G    +AWS+ RPGL++G S+RT +NFMGSLCVYG
Sbjct: 155 ENGPRVKAGCNFYYVLEDLLKERLDGK---IAWSVHRPGLLIGSSSRTLYNFMGSLCVYG 214

Query: 182 AICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT------------------- 241
            ICK LN+PFVFGGT  CWEE  IDGSDARL AEQH+WAAT                   
Sbjct: 215 TICKHLNLPFVFGGTKKCWEETCIDGSDARLVAEQHIWAATNDDVNVQSTDGQSFNAING 274

Query: 242 -----KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMG 301
                K IW A+AEK G+    E+   FS +F Y+ AMA+K  VW EIV +E ++ T+M 
Sbjct: 275 PSFTWKEIWTALAEKFGLEVVPENA--FSEDFWYSKAMADKKEVWKEIVAKERMMKTEME 334

Query: 302 ELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           +LANW FLD LFR P K++ +R K D+LGFTV+YK LDSILYWID MR +KLIP
Sbjct: 335 DLANWAFLDVLFRCPFKLLGTRNKVDQLGFTVRYKTLDSILYWIDCMRDEKLIP 382

[Close]
BLAST of Cp4.1LG20g08120 vs. TAIR10
Match: AT5G58750.1 (AT5G58750.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 354.0 bits (907), Expect = 1.0e-97
Identity = 182/356 (51.12%), Postives = 232/356 (65.17%), Query Frame = 1 

Query: 1   MLLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRH-VTHMFWITW 60
           +L+S  GW++YGVAR P+  S      ++FISC+LL+       LSPL+  V+H+FW+TW
Sbjct: 36  LLMSKPGWRIYGVARNPEINSMTK--MYNFISCDLLNASETKQRLSPLQDIVSHVFWVTW 95

Query: 61  AAQLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSST---GGGGG 120
           + +   D+ +C  QNK ML NALDAILP+A  L+H SLQTG+KHY SL   T   G G  
Sbjct: 96  SGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQTGMKHYVSLVEETMARGEGSS 155

Query: 121 EVVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGS 180
              Y E+ PR  S  NFYYVLEDLL+E+++  R  V WS+ RPGL++G S+RT +NFMGS
Sbjct: 156 LYYYSEECPRKSSGKNFYYVLEDLLKEKIT--RSSVVWSVQRPGLLMGSSSRTLYNFMGS 215

Query: 181 LCVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------- 240
           LCVYGA+CK LN+PFVFGGT  CWEE YIDGSD+ L AEQH++AAT              
Sbjct: 216 LCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQHIFAATSGKVREKGEAFNAI 275

Query: 241 -------KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATK 300
                  K IW  + +KLGV     +  MF   F +   M E+  VW EIV +E LV T+
Sbjct: 276 NGVGFTWKEIWPEIGKKLGVQVN--ETTMFDEGFWFGREMVERKHVWDEIVVKEKLVRTE 335

Query: 301 MGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           + +LANW FLD+LFR P K++  R K DR GF  KY+ LDS+LYWIDVMR +KLIP
Sbjct: 336 IEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLDSVLYWIDVMRDEKLIP 385

[Close]
BLAST of Cp4.1LG20g08120 vs. TAIR10
Match: AT4G24220.1 (AT4G24220.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 201.4 bits (511), Expect = 8.5e-52
Identity = 125/343 (36.44%), Postives = 172/343 (50.15%), Query Frame = 1 

Query: 8   WKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAAQLRFDS 67
           WKVYGVARRP        P   +I C++ D       LSPL  VTH+F++TW  +   +S
Sbjct: 56  WKVYGVARRPRPTWNADHP-IDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNR-ESES 115

Query: 68  QDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYCEDSPRA 127
           ++C + N +ML N L AI+P A  LRHV LQTG KHY    ++  G   +  + ED PR 
Sbjct: 116 ENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRL 175

Query: 128 ESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKL 187
           + + NFYY  ED+L E +      V WSI RP +I G S  +  N +G+LCVY AICK  
Sbjct: 176 Q-IQNFYYTQEDILFEEIKKI-ETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHE 235

Query: 188 NMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT-------------------KAIWGA 247
             P +F G+   WE  ++  SDA L AEQ +WAA                    K +W  
Sbjct: 236 GSPLLFPGSKKAWEG-FMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKI 295

Query: 248 VAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGELANWGFLDSL 307
           +AE+ G+     +E            M  K  VW E+V+E  L   K+ E+  W F D +
Sbjct: 296 LAEQFGIEEYGFEE---GKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVI 355

Query: 308 FRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
             + E M+ S  KS   GF       +S + WID  +  K++P
Sbjct: 356 LGV-EGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: gi|449455156|ref|XP_004145319.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus])

HSP 1 Score: 579.3 bits (1492), Expect = 4.2e-162
Identity = 292/362 (80.66%), Postives = 307/362 (84.81%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDRRS   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSST----GGGGGE 121
           QLR DS DCSDQN+AMLANALDAILPSA ALRHVSLQTG+KHYASLT       GGG GE
Sbjct: 95  QLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGE 154

Query: 122 VVYCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSL 181
           VVYCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILGCSNRTFFNFMGSL
Sbjct: 155 VVYCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSL 214

Query: 182 CVYGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA------------- 241
           CVYGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA             
Sbjct: 215 CVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHIWVATKAVEINATADGEAFN 274

Query: 242 -----------IWGAVAEKLGV-TAGAEDE--RMFSPEFHYTAAMAEKGTVWAEIVEEEG 301
                      IWGAVAEKLGV T GAE+E  RMFS EFHYTAAM +KG VWAEIVEEEG
Sbjct: 275 VCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEG 334

Query: 302 LVATKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLI 333
           LV T+M ELANWGFLD+LFRLPEKMVASRAKSDRLGF VKYKMLDS+LYWIDVMR+DKLI
Sbjct: 335 LVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLI 394

[Close]
BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: gi|659115081|ref|XP_008457377.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo])

HSP 1 Score: 566.2 bits (1458), Expect = 3.7e-158
Identity = 287/356 (80.62%), Postives = 300/356 (84.27%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDR+S   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGG--EVV 121
           QLR DS  CSDQN+AMLANALDAILPSA ALRHVSLQTGVKHYASLT    G GG  EVV
Sbjct: 95  QLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVV 154

Query: 122 YCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCV 181
           YCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILG SNRTFFNFMGSLCV
Sbjct: 155 YCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCV 214

Query: 182 YGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA--------------- 241
           YGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA               
Sbjct: 215 YGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWVATKAVEINDGEAFNVCNGW 274

Query: 242 ------IWGAVAEKLGV--TAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKM 301
                 IWG VAEKLGV  TA  E ERMFS EFHYTAAM +KG VWAEIVEEEGLVAT+M
Sbjct: 275 SFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVATEM 334

Query: 302 GELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 333
            ELANW FLD+LFRLPEKMVASRAKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 335 EELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 390

[Close]
BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: gi|659115079|ref|XP_008457376.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo])

HSP 1 Score: 565.1 bits (1455), Expect = 8.3e-158
Identity = 287/359 (79.94%), Postives = 300/359 (83.57%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LLSTAGWKVYGVARRPDN+SPIS P FHFISC+LLDR+S   NLSP+RHVTH+FWITWAA
Sbjct: 35  LLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRQSVQQNLSPVRHVTHLFWITWAA 94

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGG--EVV 121
           QLR DS  CSDQN+AMLANALDAILPSA ALRHVSLQTGVKHYASLT    G GG  EVV
Sbjct: 95  QLRLDSPGCSDQNRAMLANALDAILPSAPALRHVSLQTGVKHYASLTRLAAGCGGREEVV 154

Query: 122 YCEDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCV 181
           YCEDSPRAES NNFYYVLEDLLRERLS  RR+VAWS+LRPGLILG SNRTFFNFMGSLCV
Sbjct: 155 YCEDSPRAESGNNFYYVLEDLLRERLSCGRRMVAWSVLRPGLILGSSNRTFFNFMGSLCV 214

Query: 182 YGAICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKA--------------- 241
           YGAICKKL MPFVFGGTAACWEEVYIDGSDARLTAEQH+W ATKA               
Sbjct: 215 YGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHMWVATKAVEINATADGEAFNVC 274

Query: 242 ---------IWGAVAEKLGV--TAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVA 301
                    IWG VAEKLGV  TA  E ERMFS EFHYTAAM +KG VWAEIVEEEGLVA
Sbjct: 275 NGWSFRWKEIWGTVAEKLGVATTAEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVA 334

Query: 302 TKMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIPS 333
           T+M ELANW FLD+LFRLPEKMVASRAKSDR GF VKYKMLDSILYWIDVMR+DKLIPS
Sbjct: 335 TEMEELANWVFLDTLFRLPEKMVASRAKSDRFGFNVKYKMLDSILYWIDVMRNDKLIPS 393

[Close]
BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: gi|720053698|ref|XP_010272811.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Nelumbo nucifera])

HSP 1 Score: 391.0 bits (1003), Expect = 2.1e-105
Identity = 198/352 (56.25%), Postives = 246/352 (69.89%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LL+ +GWKVYG+AR+PD  +PI   N+ FISC+LLD       LS L   TH+FW+TWA+
Sbjct: 40  LLAKSGWKVYGIARKPDFFTPIQHRNYRFISCDLLDPVQTVQKLSSLNGATHIFWVTWAS 99

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYC 121
           Q   DS +C DQNKAM++NALDAILP A AL+HVSLQTG KHY SL+           Y 
Sbjct: 100 QFPLDSSECCDQNKAMMSNALDAILPKAKALKHVSLQTGTKHYVSLSGPFDNTVRR--YD 159

Query: 122 EDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYG 181
           E+SPR    +NFYY+LEDLL E+L G    V+WS+ RPGLILG S+RT FNF+GSLC+YG
Sbjct: 160 EESPRVNEGHNFYYMLEDLLIEKLRGK---VSWSVHRPGLILGSSHRTAFNFIGSLCIYG 219

Query: 182 AICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT------------------- 241
            ICK L++PFVFGG   CWEEVYIDGSDARL AEQH+W +T                   
Sbjct: 220 TICKHLHLPFVFGGARECWEEVYIDGSDARLVAEQHIWVSTNDNVSSTDGQAFNAVNGPS 279

Query: 242 ---KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGEL 301
              K IW A+A K G+ A AED  MFS +F ++ +MA+K +VW +IV +EGL  TK+G+L
Sbjct: 280 FTWKQIWPALAVKFGLQA-AED--MFSQDFLFSTSMADKESVWEDIVVKEGLRPTKIGDL 339

Query: 302 ANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           ANW FLD LFR P K++A+R K DRLGFT+ Y+MLDSILYW+D +R +KLIP
Sbjct: 340 ANWTFLDVLFRFPTKLLAARDKVDRLGFTMTYEMLDSILYWVDCIRDEKLIP 383

[Close]
BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match: gi|720053693|ref|XP_010272810.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Nelumbo nucifera])

HSP 1 Score: 391.0 bits (1003), Expect = 2.1e-105
Identity = 198/352 (56.25%), Postives = 246/352 (69.89%), Query Frame = 1 

Query: 2   LLSTAGWKVYGVARRPDNISPISDPNFHFISCNLLDRRSAHLNLSPLRHVTHMFWITWAA 61
           LL+ +GWKVYG+AR+PD  +PI   N+ FISC+LLD       LS L   TH+FW+TWA+
Sbjct: 43  LLAKSGWKVYGIARKPDFFTPIQHRNYRFISCDLLDPVQTVQKLSSLNGATHIFWVTWAS 102

Query: 62  QLRFDSQDCSDQNKAMLANALDAILPSALALRHVSLQTGVKHYASLTSSTGGGGGEVVYC 121
           Q   DS +C DQNKAM++NALDAILP A AL+HVSLQTG KHY SL+           Y 
Sbjct: 103 QFPLDSSECCDQNKAMMSNALDAILPKAKALKHVSLQTGTKHYVSLSGPFDNTVRR--YD 162

Query: 122 EDSPRAESVNNFYYVLEDLLRERLSGYRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYG 181
           E+SPR    +NFYY+LEDLL E+L G    V+WS+ RPGLILG S+RT FNF+GSLC+YG
Sbjct: 163 EESPRVNEGHNFYYMLEDLLIEKLRGK---VSWSVHRPGLILGSSHRTAFNFIGSLCIYG 222

Query: 182 AICKKLNMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAAT------------------- 241
            ICK L++PFVFGG   CWEEVYIDGSDARL AEQH+W +T                   
Sbjct: 223 TICKHLHLPFVFGGARECWEEVYIDGSDARLVAEQHIWVSTNDNVSSTDGQAFNAVNGPS 282

Query: 242 ---KAIWGAVAEKLGVTAGAEDERMFSPEFHYTAAMAEKGTVWAEIVEEEGLVATKMGEL 301
              K IW A+A K G+ A AED  MFS +F ++ +MA+K +VW +IV +EGL  TK+G+L
Sbjct: 283 FTWKQIWPALAVKFGLQA-AED--MFSQDFLFSTSMADKESVWEDIVVKEGLRPTKIGDL 342

Query: 302 ANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDVMRHDKLIP 332
           ANW FLD LFR P K++A+R K DRLGFT+ Y+MLDSILYW+D +R +KLIP
Sbjct: 343 ANWTFLDVLFRFPTKLLAARDKVDRLGFTMTYEMLDSILYWVDCIRDEKLIP 386

The following BLAST results are available for this feature:
BLAST of Cp4.1LG20g08120 vs. Swiss-Prot
Match NameE-valueIdentityDescription
VEP1_ARATH1.5e-5036.443-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 S... [more]
5BPOR_DIGLA1.8e-4836.343-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1[more]
IRIS_CATRO3.5e-4735.34Iridoid synthase OS=Catharanthus roseus PE=1 SV=1[more]
YJ72_SCHPO2.7e-0731.45Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
BLAST of Cp4.1LG20g08120 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LVI2_CUCSA2.9e-16280.66Uncharacterized protein OS=Cucumis sativus GN=Csa_1G530150 PE=4 SV=1[more]
A0A022RYY0_ERYGU8.2e-10454.78Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a018609mg PE=4 SV=1[more]
A0A0K9S2H9_SPIOL1.5e-10254.62Uncharacterized protein OS=Spinacia oleracea GN=SOVF_002350 PE=4 SV=1[more]
A0A103XNN3_CYNCS5.8e-10253.57NAD(P)-binding domain-containing protein OS=Cynara cardunculus var. scolymus GN=... [more]
M5W676_PRUPE7.6e-10254.52Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021134mg PE=4 SV=1[more]
BLAST of Cp4.1LG20g08120 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G58750.11.0e-9751.12 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT4G24220.18.5e-5236.44 NAD(P)-binding Rossmann-fold superfamily protein[more]
BLAST of Cp4.1LG20g08120 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449455156|ref|XP_004145319.1|4.2e-16280.66PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus][more]
gi|659115081|ref|XP_008457377.1|3.7e-15880.62PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Cucumis melo][more]
gi|659115079|ref|XP_008457376.1|8.3e-15879.94PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Cucumis melo][more]
gi|720053698|ref|XP_010272811.1|2.1e-10556.25PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X2 [Nelumbo nucifer... [more]
gi|720053693|ref|XP_010272810.1|2.1e-10556.25PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase isoform X1 [Nelumbo nucifer... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016040NAD(P)-bd_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050662 coenzyme binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG20g08120.1Cp4.1LG20g08120.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 4..181
score: 2.
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 3..227
score: 9.17
NoneNo IPR availablePANTHERPTHR32487FAMILY NOT NAMEDcoord: 5..333
score: 2.5E
NoneNo IPR availablePANTHERPTHR32487:SF7SUBFAMILY NOT NAMEDcoord: 5..333
score: 2.5E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG20g08120Cucsa.147030Cucumber (Gy14) v1cgycpeB0389
Cp4.1LG20g08120CmaCh13G000690Cucurbita maxima (Rimu)cmacpeB235
Cp4.1LG20g08120CmaCh01G006600Cucurbita maxima (Rimu)cmacpeB483
Cp4.1LG20g08120CmoCh01G006910Cucurbita moschata (Rifu)cmocpeB441
Cp4.1LG20g08120CmoCh13G000580Cucurbita moschata (Rifu)cmocpeB202
Cp4.1LG20g08120Cla008168Watermelon (97103) v1cpewmB507
Cp4.1LG20g08120Csa1G530150Cucumber (Chinese Long) v2cpecuB507
Cp4.1LG20g08120MELO3C020553Melon (DHL92) v3.5.1cpemeB479
Cp4.1LG20g08120ClCG03G000790Watermelon (Charleston Gray)cpewcgB467
Cp4.1LG20g08120CSPI01G23840Wild cucumber (PI 183967)cpecpiB507
Cp4.1LG20g08120Lsi02G012870Bottle gourd (USVL1VR-Ls)cpelsiB422
Cp4.1LG20g08120MELO3C020553.2Melon (DHL92) v3.6.1cpemedB561
Cp4.1LG20g08120CsaV3_1G036150Cucumber (Chinese Long) v3cpecucB0625
Cp4.1LG20g08120Bhi08G000424Wax gourdcpewgoB0650
Cp4.1LG20g08120CsGy1G023320Cucumber (Gy14) v2cgybcpeB071
Cp4.1LG20g08120Carg12426Silver-seed gourdcarcpeB0620
Cp4.1LG20g08120Carg20604Silver-seed gourdcarcpeB0531
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cp4.1LG20g08120Cp4.1LG02g11730Cucurbita pepo (Zucchini)cpecpeB430
The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG20g08120Cucurbita pepo (Zucchini)cpecpeB387
Cp4.1LG20g08120Cucurbita pepo (Zucchini)cpecpeB444
Cp4.1LG20g08120Cucurbita pepo (Zucchini)cpecpeB447
Cp4.1LG20g08120Cucumber (Gy14) v1cgycpeB0376
Cp4.1LG20g08120Cucurbita maxima (Rimu)cmacpeB636
Cp4.1LG20g08120Cucurbita maxima (Rimu)cmacpeB742
Cp4.1LG20g08120Cucurbita moschata (Rifu)cmocpeB586
Cp4.1LG20g08120Cucurbita moschata (Rifu)cmocpeB694
Cp4.1LG20g08120Cucurbita moschata (Rifu)cmocpeB695
Cp4.1LG20g08120Bottle gourd (USVL1VR-Ls)cpelsiB412
Cp4.1LG20g08120Watermelon (Charleston Gray)cpewcgB462
Cp4.1LG20g08120Watermelon (97103) v1cpewmB526
Cp4.1LG20g08120Cucumber (Gy14) v2cgybcpeB670
Cp4.1LG20g08120Cucumber (Gy14) v2cgybcpeB818
Cp4.1LG20g08120Silver-seed gourdcarcpeB0942
Cp4.1LG20g08120Cucumber (Chinese Long) v3cpecucB0648
Cp4.1LG20g08120Wax gourdcpewgoB0628