MELO3C017002 (gene) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MELO3C017002
Type	gene
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Alpha-amylase
Location	chr7 : 511759 .. 516775 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGCCCACGCGGCTGCAGATCAATTGCCTGATTGCAAATGGTTCGAACAGATAAAATCGTTTCCCTGAACCAGCAGAACCTGGAACGTCGAACCGCGAAGTGATTGCTTCTTGGTAACGTTTTTCATTATTTGCTTAGTAACTTTTATTCATGATCGCCTTATGTTCTTGCGACGTTCAAATGCGGAATTCTAATCGCATGGCTTAGTGAAGCGTTTTGGTTGTTTAGCTATTTTCCCATTAATAAATATGTATACACGACGAATCACTCTCAAAGCATCTTTCGTTGTAATTGCGTTCTTCTTTTATTTTTTCTGATACGAAGATTCTTGCTAGCTGATCTTATCTCTTTCTTGAATTTTTCAGCTGTAAATGTCTTCAAGCCAATCTGAAACTATGATTTCAGCTGATTAATTCGTATTGTCAAGGTTGTGAACGCATCTGCTGCATAGCTACTTAGAAGTTGATTTTGGACAATTTTAAGAAGTGTGAGTGGTTTATCCAGTTTGAAATTGAGGAATAGATTCTCAAAATGAAGCTAGAGCCATGAATAGTCCCCAGTCCCACCATGTCACTAAGTATTCTGTTATAATTCTAGCTCGTTATCTTATTTTCTTTTTCGTTGTTCGAGTGGCCATACGGGTAATGAAGTTAAGACCACACAATATGAATGAGGTTACACTTTGCAGATTGTGATGCTTTTTGATTTTGGTTGTAGGACTAGGACGTCCCTATCTTCTGCAACCAAGACGACAAGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAATGTATGCTTCTTGTTTAATTTGGAAATTGTTTCCTTGATGAGTTTTCTGCATCAGTGTGATATGCTTTTAGTTATAAATTTGTCGGAATTAGAATATAGATTCTGATATACAAATTACTTATTTATGTTGGAATTCAGGTTTTCGATGAAAACTACGAACAGACTGATACTGGTATGGTTTTTTCTTTGTAAATTTCACATCATGGTATGCTCACGTCGATCTTAAGTGTTTGTCAAAAGATCATACCTCTCTTGGAAGTGACTTCAAACTCTAATACATTTGCTTGGTCTAACTAACTAAAGAGAATGTTTGATGATGCTCAAAATTTGACTCAGGGATAAGAAATTTGAAAAGAAATATATGCTTCTTAGGTGTGAGTACGTTGCAATATTTGCTTTTAGTTTACATAAAATTTGTGGTTTATGAAAAGGATTGAAACACGTGGATTTGAAATCAGAGCTGGATTTCTGCTACGTAAATTTTCTTTTGTTTGAAATTATTTTATCGTTTTAATAATTTCAGTGTTTCTTTATGGTCACCTGTAGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGTAGAGGTTACAATTTCAGCTCTCTGATTTTTCTCTAGAGCAGAGGTCAGCAGGAAGGAAAATATTATCTTGCTCTTAATTGAGCCGAAGTCTCGGAAACAGAAAAAGAAAAAAATGTTTGCCGTTTGAAATTCATGCTCGATGTTGTTGATTCTTTTAATGTAAATCGGCTAGAATTTGAGGTGGAAGGACTTCGGGACTGTCAATGTTTTGGATCAAATCTCCAGGTCATGCTAAACTTATGGGAGAAATTTTGCACCCATAATTTCTATTGTAATTACAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTCAAAGGAACAAATTCACTTGAATTTTTATGTTCCTTTCACATATATGCGTGCATAACTCAATATTTTCTTCACAATTTCAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGTATTTTTCTGTTACAAATTCCAAGTGAGCTCATAATTATAAGAAAACCTTTGGTCTTTAGTCTGTGGTATCTGTTCTAGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGGTAATATTGTCCTTCTCTGTTTGTGTGTGTGTGTAAATATATTGTGGGGAGGCTGGGGCGTTACAGAGGTTCAAGGATAAATTGTTCTGAAAATGACAAGATCCCTCCTCTGTTTCAAAATTTCACTGTATCATCTTCTCAACATTCGGCTTGTCTTTTCTTGTAAGGAGTTCTTCAATGGATTATAACATACATTTGCCGTCTGTCTTTTTTTTTTTTTTTGATGCTTTAACTCAGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGGTAACATGATGATGCATTATGTTGATAAATTGAAATCATGTTTTCAAGTTTCAGGATTTTTAGTTTTTTTGAATGGTCTTGTCTGTGAATAATATCCTTTGTTCGTCTTATCAATTCATGTGTTCCATAGTGATGATTACAGCTCTAAATGCAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGTATTGGTGTTTTGTGAGGCCATTGCTAACTTCCTTCCCTCCTAAAAATTGTAATACTACGGAGCTCAAATCTTAATTACTTAAAATAGAAAGGATGAAGTGACAGCTATGTCATGGGTTTTTTCCCCTCTAGATCAGTAGATCCACAACATTTGACATTTTACAACTCTTTCTATTTGCTTCTTTTCTCCTTCTTTATTAAGTGAAGTTTGGGTTTTGTTAATATTTGCTCTTGCTAGTACATTGCTTGACTAACATGCATTTGTTTCCTTGTTTGCTTAGTAATAAACATAGTATAATTAATTTTGCTTGTAGGAAGCTGTTAAAGGACAATTATGGCGTCTGTGTGACTGTCGAGGAAAGCCACCCGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGTACCATGTCTTCAAATCTGTTCGTGAAATTATTGGTTGACAAATTTCAACATTTGTTTTCATTGCTCATTGTGCATGTGCCTTTTAAAATTGTCTCAACTTGACCGCGTATCCATGTCCTTTTATTTGGATTAATATTTGACAACACGTATTTGGAAGTCATGCCCTGTATTGGGGATTGAGTGTGACCAGACCATACATGAAAATGTCATAGGAAGCAAGGGAGAAAGGAAGTATTGATGCATACCTGAATGAAGTTGTAATGTCTGGTTGTTCTACACGGCTTCTATATGGCCAAATATGATGCCATCAGAACAGCTACTGGACTCTGTTCCATTTTTCTATTTTTCTATTATCTTTTGTAAGATTCGTATGTGTTTTTTGCAACATGTAACTGAGATTTATTGGCCAAGCTTTCTTTCATATAGGTTTGTTCAAATTGAACTAATTAAAATGTAAATAGTTCTAGGCTTCATCCAACTCTGCATCTTATTGAGTAGATAGCTTTAGATTATTATTCGGAAACTCTTAATTAAAATTTTTTTTTCCTAGGTTGAAAGCAAGCATCAAGTGTGCTTTCAAGTATAGATATTGACTTGAGCAGATCATGACAATTAATGCAACTCGACATGCTTGGCAAATGAGCTATATAATTCTTCATATGTATTTTCTTGAAACATTTATAGAAGGTGAAATTGTACCATTAGAATGTTGGTGACTCCACTAACCTCAGCGTACCATTGTTCTTGCAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGTATGCAAGTGTGGCATATCATTCCATTTGCAATCTCTTTATTGCTTATAGTCTTATTCTTGCTGCTGTGGCTTCATCGTGGTTTTTGGAAGCTCTCAATTGTTTGGTCCAATTATTCGTTGTGTAAAGTCTAATTATCCCCCATCAGGTGGTCCTCATTGTTCCCTGTGTGTTTTGGAAGCTTTCAATTGTTTGTTCCAATTGATCCTATGTATGACTGTCTATACATTTGAAGTTAGTTTCCTACTTTTAGCTGCAAATTCTATGGCTATAACTGTTCAGTATAAATTTAAAAAAAGAAAAAGAAAAAAAAAAAAAACTGTTCAGCATACTAAGCAATCAATCTTTGATGTTTCTGGGTGAATTTCGTTGAAAGAGCTTTATAATTACTTTTATGCTTGGCCTAGAGTCGTCACTTGTCTTCCAGTAAAAAAAATTCCGTCATGTTTCTATTTTCTGATTCCTGGAGCTTTGGTTATTCCCTGCAGGGCTATGCATATATTCTCACACACCCAGGGATACCAACAGTATTTTATGACCACTTTTACGATTGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAGTATGTGGCATAGTCAATAATGCTTCAATAGAGTTTGTTATCTTCTATTCAAGCAAACTTCTCATAGTACTTAAAATTTTATTTTTCTTGCAGATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAACTTGTGCATGAAAATCGGAGATGGTTCTTGGTGTCCTGGTACGGAATGGACTCTTGCAACCTATGGATGCAGATATGCAGTGTGGCAGAAATAA

mRNA sequence

GAGCCCACGCGGCTGCAGATCAATTGCCTGATTGCAAATGGTTCGAACAGATAAAATCGTTTCCCTGAACCAGCAGAACCTGGAACGTCGAACCGCGAAGTGATTGCTTCTTGCTGTAAATGTCTTCAAGCCAATCTGAAACTATGATTTCAGCTGATTAATTCGTATTGTCAAGGTTGTGAACGCATCTGCTGCATAGCTACTTAGAAGTTGATTTTGGACAATTTTAAGAAGTGACTAGGACGTCCCTATCTTCTGCAACCAAGACGACAAGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAATGTTTTCGATGAAAACTACGAACAGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAAAGGACAATTATGGCGTCTGTGTGACTGTCGAGGAAAGCCACCCGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACACACCCAGGGATACCAACAGTATTTTATGACCACTTTTACGATTGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAACTTGTGCATGAAAATCGGAGATGGTTCTTGGTGTCCTGGTACGGAATGGACTCTTGCAACCTATGGATGCAGATATGCAGTGTGGCAGAAATAA

Coding sequence (CDS)

ATGGGTGACCATGATAATGTTTTCGATGAAAACTACGAACAGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCAAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTACGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTTTTCTGCAAAATTCGTGAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGGGAATATTGGGATTCTTGTAATTATAATGGCCATGACTTGGACTATAATCAAGATGGACATAGACAGCGGATAATAAATTGGATTGATGGCACAGGACAACTTTCAGCTGCTTTTGATTTTACAACTAAGGGAGTCCTTCAGGAAGCTGTTAAAGGACAATTATGGCGTCTGTGTGACTGTCGAGGAAAGCCACCCGGTGTGATGGGATGGTGGCCTTCAAGGGCCGTGACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCCTTTCCTGCCAACCATATCATGGAGGGCTATGCATATATTCTCACACACCCAGGGATACCAACAGTATTTTATGACCACTTTTACGATTGGGGTGACTCAGTGCACGACGAGATAGTAAAATTAATAGACATTCGAAAACATCAAGACATCAACAGTCGGTCATCTGTGAAGATCCTCGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAACTTGTGCATGAAAATCGGAGATGGTTCTTGGTGTCCTGGTACGGAATGGACTCTTGCAACCTATGGATGCAGATATGCAGTGTGGCAGAAATAA

Protein sequence

MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK*

Homology

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BLAST of MELO3C017002 vs. Swiss-Prot
Match: AMY2_ARATH (Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 2.4e-207
Identity = 326/413 (78.93%), Postives = 373/413 (90.31%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG ++NVFDE  +QTD G VIR+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVKGQ WRLCD +GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRS+V++L+A S+LY+AI+GE +CMK+GDGSWCP G +WTLAT G RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

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BLAST of MELO3C017002 vs. Swiss-Prot
Match: AMY3_ARATH (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.4e-114
Identity = 198/397 (49.87%), Postives = 262/397 (65.99%), Query Frame = 1


Query: 23  NGREILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q FNWES+K   W   L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L WD+ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCSG---GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAV-KGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+ K + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH +      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGENLCMKIGDGSWCP---GTEWTLATYGCRYAVWQ 412
           II E + MKIG G + P      W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

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BLAST of MELO3C017002 vs. Swiss-Prot
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.8e-101
Identity = 184/402 (45.77%), Postives = 252/402 (62.69%), Query Frame = 1


Query: 26  EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           ++LFQ FNWES +    W   L  KV +IA +G T  WLPPP+HS++PQGY+P  LY L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 86  SS-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGIL----LPWDE 145
           +S YG+E  LK+L+       V  +ADIVINHR        G++  F+G      L W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 146 HAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG  F   P+IDH  P V+R++  WL WLR+ +GF  +R DF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 206 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDL-DYNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++   P F +GE W S  YNG      NQD  RQ ++NW++G G+ + 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 266 AFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+G+LWRL D  GK PG+MGW P +AVTF+DNHDTGSTQ+ WPFP++
Sbjct: 266 AFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSD 325

Query: 326 HIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSD 385
            +M+GYAYILTHPGIP +FYDH +DW  ++  EI  L +IR    I++ S++ IL+A  D
Sbjct: 326 KVMQGYAYILTHPGIPCIFYDHVFDW--NLQHEIATLAEIRSRNGIHAESTLDILKAEGD 385

Query: 386 LYSAIIGENLCMKIG---DGSWCPGTEWTLATYGCRYAVWQK 413
           +Y A+I   +  K+G   D      +++ +  +G  Y VW+K
Sbjct: 386 IYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 425

[Close]

BLAST of MELO3C017002 vs. Swiss-Prot
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.9e-101
Identity = 184/401 (45.89%), Postives = 258/401 (64.34%), Query Frame = 1


Query: 27  ILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSS 86
           +LFQ FNWES K   W  +L+  + D+A +G T  WLPPP+ S++P+GYLP  LY L++S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 87  -YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDEHA 146
            YGS++ LK+L+    +  ++ +ADIVINHR        G++  F+G        W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 147 V----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
           +    T+ S G GN  +G+ +   P+IDH  P V+R++  W+ WL++ +GF  +RFDF +
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 207 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHD--LDYNQDGHRQRIINWIDGTGQLSAA 266
           G++    K Y+E  KP F++GE WDS +Y G D   +YNQD HR  ++NW++  G    A
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVNWVESAGGAITA 264

Query: 267 FDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AV+G+LWRL D  GKPPG++G  P  AVTF+DNHDTGSTQ  WPFP++ 
Sbjct: 265 FDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 327 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 386
           +M+GYAYILTHPG P++FYDHF+DWG  + ++I KL  IR    IN +S+VKI+ +  DL
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNGINEKSTVKIMASEGDL 384

Query: 387 YSAIIGENLCMKIGD----GSWCPGTEWTLATYGCRYAVWQ 412
           Y A I   + +KIG     G+  P +   +AT G  YAVW+
Sbjct: 385 YVAKIDNKIMVKIGPKMDLGNLIP-SNLHVATSGQDYAVWE 421

[Close]

BLAST of MELO3C017002 vs. Swiss-Prot
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 2.0e-100
Identity = 183/403 (45.41%), Postives = 257/403 (63.77%), Query Frame = 1


Query: 26  EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           +ILFQ FNW+S K    W   L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 86  SS-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGI----LLPWDE 145
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 146 HAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG +F   P+IDH  P V+R++  WL+WLR  VGF  +R DF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208

Query: 206 ARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDY-NQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++ A+P F + E W+S +Y+G      NQDG RQ ++NW+   G  + 
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268

Query: 266 AFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+G+LWR+ D  GK PG++GW+P +AVTF+DNHDTGSTQ  WPFP++
Sbjct: 269 AFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSD 328

Query: 326 HIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSD 385
            ++ GYAYILTHPG+P +FYDH +DW  ++  EI  L   RK   IN+ S +++L A SD
Sbjct: 329 KVILGYAYILTHPGVPCIFYDHVFDW--NLKQEINALAATRKRNGINAGSKLRVLAAESD 388

Query: 386 LYSAIIGENLCMKIGD----GSWCPGTEWTLATYGCRYAVWQK 413
           +Y A++ E +  KIG     G+  P +++ +  +G  Y VW+K
Sbjct: 389 MYVAMVDERVITKIGPRIDVGNIIP-SDFHIVAHGNDYCVWEK 428

[Close]

BLAST of MELO3C017002 vs. TrEMBL
Match: A0A0A0L5R6_CUCSA (Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 7.2e-235
Identity = 387/413 (93.70%), Postives = 396/413 (95.88%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

[Close]

BLAST of MELO3C017002 vs. TrEMBL
Match: M5XLS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 9.8e-208
Identity = 337/413 (81.60%), Postives = 369/413 (89.35%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG   N  +EN +QTD GA +RNGREILFQAFNWESHK DW  NLE KV D+ +SGFTSA
Sbjct: 1   MGHRGNDSNENSQQTDIGAALRNGREILFQAFNWESHKHDWWRNLERKVPDMGRSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPP THS AP+GYLPQNLYSLNS YGSE +LK LL KMKQ KVR MADIVINHRVGT  
Sbjct: 61  WLPPATHSFAPEGYLPQNLYSLNSKYGSEHLLKGLLQKMKQHKVRPMADIVINHRVGTTQ 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI L WDEHAVTSC+GGLGNRSTGDNFHGVPNIDHTQ FVR+DI AWL+W
Sbjct: 121 GHGGMYNRYDGISLSWDEHAVTSCTGGLGNRSTGDNFHGVPNIDHTQLFVRKDITAWLQW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SAK+VKEYIE AKP+FS+GEYWDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNNVGFQDFRFDFARGYSAKYVKEYIEGAKPIFSVGEYWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDG GQLS AFDFTTKG+LQEAVKGQLWRL D +GKPPGV+GWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGAGQLSTAFDFTTKGILQEAVKGQLWRLRDPQGKPPGVIGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP+NHIMEGYAYILTHPGIP+VFYDHFYDW DS+HD+IVKLIDIRKHQDI+
Sbjct: 301 TGSTQAHWPFPSNHIMEGYAYILTHPGIPSVFYDHFYDWSDSIHDQIVKLIDIRKHQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRSS++ILEA+ +LYSAIIGE +CMKIGD SWCP G EWTLAT G RYA+W K
Sbjct: 361 SRSSIRILEAQPNLYSAIIGEKVCMKIGDSSWCPTGREWTLATCGHRYAIWHK 413

[Close]

BLAST of MELO3C017002 vs. TrEMBL
Match: A0A067HB02_CITSI (Alpha-amylase OS=Citrus sinensis GN=CISIN_1g014892mg PE=3 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 4.1e-206
Identity = 335/406 (82.51%), Postives = 368/406 (90.64%), Query Frame = 1


Query: 8   FDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTH 67
           FDE  +QTD GAVIRNGREILFQ FNWES K DW  NLE KV DI+KSGFTS WLPP TH
Sbjct: 8   FDETNQQTDLGAVIRNGREILFQGFNWESCKHDWWRNLERKVPDISKSGFTSVWLPPATH 67

Query: 68  SIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHN 127
           S AP+GYLPQNLYSLNSSYGSE +LKALL KMKQ KVRAMADIVINHRVGT  GHGG +N
Sbjct: 68  SFAPEGYLPQNLYSLNSSYGSEHLLKALLHKMKQHKVRAMADIVINHRVGTTQGHGGKYN 127

Query: 128 RFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGF 187
           R+DGI L WDEHAVTSC+GGLGN STGDNFHGVPNIDHTQ FVR+DIIAWL+WLR++VGF
Sbjct: 128 RYDGIPLSWDEHAVTSCTGGLGNGSTGDNFHGVPNIDHTQHFVRKDIIAWLRWLRNTVGF 187

Query: 188 QDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDG 247
           QDFRFDFARG+SAK+VKEYIE A+P+FS+GEYWDSCNYN H LDYNQD HRQRIINWIDG
Sbjct: 188 QDFRFDFARGYSAKYVKEYIEGARPIFSVGEYWDSCNYNSHGLDYNQDSHRQRIINWIDG 247

Query: 248 TGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAH 307
           TGQLSAAFDFTTKG+LQEAVKGQ WRL D +GKPPGVMGWWPSRAVTFLDNHDTGSTQAH
Sbjct: 248 TGQLSAAFDFTTKGILQEAVKGQFWRLRDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAH 307

Query: 308 WPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKI 367
           WPFP+NHIMEGYAYIL HPGIP+VFYDHFYDWGDS+H++IVKL+D+R+ QDI+SRSS+KI
Sbjct: 308 WPFPSNHIMEGYAYILMHPGIPSVFYDHFYDWGDSIHNQIVKLMDVRRQQDIHSRSSIKI 367

Query: 368 LEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           LEA+S+LYSAIIG+ +CMKIGDGSWCP G EWTLAT G +YAVW K
Sbjct: 368 LEAQSNLYSAIIGDKVCMKIGDGSWCPAGKEWTLATSGHKYAVWHK 413

[Close]

BLAST of MELO3C017002 vs. TrEMBL
Match: D7KTA0_ARALL (Alpha-amylase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 9.2e-206
Identity = 327/413 (79.18%), Postives = 375/413 (90.80%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG H+NVFDE  +QTD G V+R+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYHNNVFDECNDQTDIGRVLRDGREVILQAYNWESHKHDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVKGQ WRLCD +GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP++HIMEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHIMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRS++++L+A S+LY+AI+GE LCMK+GDGSWCP G +WTLAT G RYAVW K
Sbjct: 361 SRSTIRVLKAESNLYAAIVGEKLCMKLGDGSWCPSGGDWTLATSGHRYAVWHK 413

[Close]

BLAST of MELO3C017002 vs. TrEMBL
Match: R0IAQ9_9BRAS (Alpha-amylase OS=Capsella rubella GN=CARUB_v10020375mg PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 1.7e-204
Identity = 326/413 (78.93%), Postives = 374/413 (90.56%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG ++NVFD+  +QTD G V+R+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDQCNDQTDIGRVLRDGREVILQAYNWESHKHDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLHKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DG+ LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGLSLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQQFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSC YNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCKYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWID TGQ+SAAFDFTTKG+LQEAVKGQ WRLCD +GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDSTGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP++HIMEGYAYILTHPGIP+VFYDHFYDWG S HD+IVKLIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHIMEGYAYILTHPGIPSVFYDHFYDWGSSFHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRS+++IL+A S+LY+AIIGE LCMK+GDGSWCP G +WTLAT G RYAVW K
Sbjct: 361 SRSTIRILKAESNLYAAIIGEKLCMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of MELO3C017002 vs. TAIR10
Match: AT1G76130.1 (AT1G76130.1 alpha-amylase-like 2)

HSP 1 Score: 722.6 bits (1864), Expect = 1.4e-208
Identity = 326/413 (78.93%), Postives = 373/413 (90.31%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MG ++NVFDE  +QTD G VIR+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI AAKP+FS+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAVKGQ WRLCD +GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDHFYDWG S+HD+IVKLIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRS+V++L+A S+LY+AI+GE +CMK+GDGSWCP G +WTLAT G RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of MELO3C017002 vs. TAIR10
Match: AT1G69830.1 (AT1G69830.1 alpha-amylase-like 3)

HSP 1 Score: 413.7 bits (1062), Expect = 1.3e-115
Identity = 198/397 (49.87%), Postives = 262/397 (65.99%), Query Frame = 1


Query: 23  NGREILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q FNWES+K   W   L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L WD+ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCSG---GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAV-KGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+ K + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH +      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGENLCMKIGDGSWCP---GTEWTLATYGCRYAVWQ 412
           II E + MKIG G + P      W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of MELO3C017002 vs. TAIR10
Match: AT4G25000.1 (AT4G25000.1 alpha-amylase-like)

HSP 1 Score: 342.4 bits (877), Expect = 3.8e-94
Identity = 182/402 (45.27%), Postives = 248/402 (61.69%), Query Frame = 1


Query: 27  ILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 86
           +LFQ+FNWES K +  +  +L   + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 87  S-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGIL----LPWDEH 146
           S YGSE  LK+L+  + Q  ++A+ADIVINHR          +  F+G      L WD  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 147 AVTSCSG---GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
            V        G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+ R
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 207 GFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGH-DLDYNQDGHRQRIINWI-DGTGQLSAA 266
           G+++   K Y++   P F++GE WD   Y G   LDY+Q+ HR  +  WI +  G +  A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 267 FDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AVKG+LWRL D +GKPPG++G  P  AVTF+DNHDT  T   W FP++ 
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 327 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 386
           ++ GY YILTHPG P +FY+H+ +WG  + + I KL+ IR    I S SSV I  A +DL
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEADL 386

Query: 387 YSAIIGENLCMKIGD----GSWCPGTEWTLATYGCRYAVWQK 413
           Y A+I + + MKIG     G+  P + + LA  G  +AVW+K
Sbjct: 387 YLAMIDDKVIMKIGPKQDVGTLVP-SNFALAYSGLDFAVWEK 422

[Close]

BLAST of MELO3C017002 vs. NCBI nr
Match: gi|659104363|ref|XP_008452918.1| (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 1.1e-255
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 412

[Close]

BLAST of MELO3C017002 vs. NCBI nr
Match: gi|659104355|ref|XP_008452914.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 1.1e-255
Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 437

[Close]

BLAST of MELO3C017002 vs. NCBI nr
Match: gi|307136308|gb|ADN34132.1| (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 884.8 bits (2285), Expect = 5.8e-254
Identity = 412/416 (99.04%), Postives = 412/416 (99.04%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 413
           QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCPGTEWTLATYGCRYAVWQK 416

[Close]

BLAST of MELO3C017002 vs. NCBI nr
Match: gi|778697053|ref|XP_011654246.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 830.1 bits (2143), Expect = 1.7e-237
Identity = 389/413 (94.19%), Postives = 399/413 (96.61%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIV+LIDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 413

[Close]

BLAST of MELO3C017002 vs. NCBI nr
Match: gi|778697076|ref|XP_011654253.1| (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 820.8 bits (2119), Expect = 1.0e-234
Identity = 387/413 (93.70%), Postives = 396/413 (95.88%), Query Frame = 1


Query: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEI   IDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 413
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

The following BLAST results are available for this feature:

BLAST of MELO3C017002 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AMY2_ARATH	2.4e-207	78.93	Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1	[more]
AMY3_ARATH	2.4e-114	49.87	Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1	[more]
AMY3E_ORYSJ	1.8e-101	45.77	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1	[more]
AMYA_VIGMU	3.9e-101	45.89	Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1	[more]
AMY3A_ORYSJ	2.0e-100	45.41	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2	[more]

BLAST of MELO3C017002 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L5R6_CUCSA	7.2e-235	93.70	Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1	[more]
M5XLS5_PRUPE	9.8e-208	81.60	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1	[more]
A0A067HB02_CITSI	4.1e-206	82.51	Alpha-amylase OS=Citrus sinensis GN=CISIN_1g014892mg PE=3 SV=1	[more]
D7KTA0_ARALL	9.2e-206	79.18	Alpha-amylase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=3 SV=1	[more]
R0IAQ9_9BRAS	1.7e-204	78.93	Alpha-amylase OS=Capsella rubella GN=CARUB_v10020375mg PE=3 SV=1	[more]

BLAST of MELO3C017002 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76130.1	1.4e-208	78.93	alpha-amylase-like 2	[more]
AT1G69830.1	1.3e-115	49.87	alpha-amylase-like 3	[more]
AT4G25000.1	3.8e-94	45.27	alpha-amylase-like	[more]

BLAST of MELO3C017002 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659104363\|ref\|XP_008452918.1\|	1.1e-255	100.00	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo]	[more]
gi\|659104355\|ref\|XP_008452914.1\|	1.1e-255	100.00	PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo]	[more]
gi\|307136308\|gb\|ADN34132.1\|	5.8e-254	99.04	cytosolic alpha-amylase [Cucumis melo subsp. melo]	[more]
gi\|778697053\|ref\|XP_011654246.1\|	1.7e-237	94.19	PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis sativus]	[more]
gi\|778697076\|ref\|XP_011654253.1\|	1.0e-234	93.70	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006046	Alpha_amylase
IPR006047	Glyco_hydro_13_cat_dom
IPR012850	A-amylase_bs_C
IPR013775	A-amylase_pln
IPR013780	Glyco_hydro_b
IPR013781	Glycoside hydrolase, catalytic domain
IPR015902	Glycoside hydrolase, family 13
IPR017853	Glycoside_hydrolase_SF

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0043169	cation binding
GO:0004556	alpha-amylase activity
GO:0005509	calcium ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
MU52206	melon EST collection version 4.0	transcribed_cluster
MU63309	melon EST collection version 4.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MELO3C017002T1	MELO3C017002T1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
MU52206	MU52206	transcribed_cluster
MU63309	MU63309	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 103..114 score: 5.6E-5coord: 187..198 score: 5.6E-5coord: 290..302 score: 5.
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 52..114 score: 1.
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 26..366 score: 1.4
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 357..411 score: 5.1
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 355..412 score: 6.8
IPR013775	Alpha-amylase, plant	PIR	PIRSF001028	Alpha-amylase_plant	coord: 6..412 score: 7.5E
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	G3DSA:2.60.40.1180		coord: 359..412 score: 7.1
IPR013781	Glycoside hydrolase, catalytic domain	GENE3D	G3DSA:3.20.20.80		coord: 23..358 score: 2.7
IPR015902	Glycoside hydrolase, family 13	PANTHER	PTHR10357	ALPHA-AMYLASE	coord: 26..412 score: 1.8E
IPR017853	Glycoside hydrolase superfamily	unknown	SSF51445	(Trans)glycosidases	coord: 40..345 score: 5.39
None	No IPR available	PANTHER	PTHR10357:SF118	ALPHA-AMYLASE 2-RELATED	coord: 26..412 score: 1.8E
None	No IPR available	unknown	SSF51011	Glycosyl hydrolase domain	coord: 359..412 score: 6.47

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
MELO3C017002	Csa4G652880	Cucumber (Chinese Long) v2	cumeB313
MELO3C017002	Bhi09G002425	Wax gourd	mewgoB595
MELO3C017002	CSPI04G26680	Wild cucumber (PI 183967)	cpimeB311
MELO3C017002	Cucsa.271900	Cucumber (Gy14) v1	cgymeB454
MELO3C017002	CsGy4G025000	Cucumber (Gy14) v2	cgybmeB270
MELO3C017002	CsaV3_4G037030	Cucumber (Chinese Long) v3	cucmeB316

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
MELO3C017002	Silver-seed gourd	carmeB0107
MELO3C017002	Silver-seed gourd	carmeB0363
MELO3C017002	Watermelon (97103) v2	mewmbB481
MELO3C017002	Cucurbita maxima (Rimu)	cmameB037
MELO3C017002	Cucurbita maxima (Rimu)	cmameB442
MELO3C017002	Cucurbita moschata (Rifu)	cmomeB026
MELO3C017002	Cucurbita moschata (Rifu)	cmomeB434
MELO3C017002	Watermelon (Charleston Gray)	mewcgB476
MELO3C017002	Watermelon (97103) v1	mewmB524
MELO3C017002	Cucurbita pepo (Zucchini)	cpemeB531
MELO3C017002	Cucurbita pepo (Zucchini)	cpemeB731
MELO3C017002	Bottle gourd (USVL1VR-Ls)	lsimeB323