MU52206 (transcribed_cluster) Melon (DHL92) v3.5.1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	MU52206
Type	transcribed_cluster
Organism	Cucumis melo (Melon (DHL92) v3.5.1)
Description	Alpha-amylase
Location	chr7 : 514494 .. 516776 (-)
Sequence length	843

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GAGCCCACGCGGCTGCAGATCAATTGCCTGATTGCAAATGGTTCGAACAGATAAAATCGTTTCCCTGAACCAGCAGAACCTGGAACGTCGAACCACGAAGTGATTGCTTCTTGCTGTAAATGTCTTCAAGCCAATCTGAAACTATGATTTCAGCTGATTAATTCGTATTGTCAAGGTTGTGAACGCATCTGCTGCATAGCTACTTAGAAGTTGATTTTGGACAATTTTAAGAAGTGGCTAGGACGTCCCTATCTTCTGCAACCAAGACGACAAGGTTTTCACAAGTTTCTCCCAAGTATTATGGGTGACCATGATAATGTTTTCGATGAAAACTACGAACGGACTGATACTGGGGCAGTGATACGCAATGGAAGAGAAATCCTTTTCCAGGCATTCAACTGGGAATCTCATAAATGTGATTGGTCGGGAAATCTAGAGTGTAAAGTTCATGATATCGCAAAAAGTGGATTTACCTCTGCATGGTTGCCTCCTCCAACTCATTCCATTGCTCCTCAAGGTTACCTCCCTCAGAACCTTTACTCCCTAAACTCTTCATACGGTTCTGAGGACATGCTGAAAGCTTTACTTCTCAAAATGAAGCAGTGCAAGGTTAGAGCAATGGCTGACATAGTCATCAATCATCGTGTTGGAACTGTTGTAGGACATGGAGGAATGCATAATCGCTTTGATGGAATACTATTACCATGGGATGAGCATGCTGTTACCTCGTGTTCTGGTGGCCTGGGCAATCGTAGCACTGGTGACAACTTCCATGGGGTTCCCAACATTGATCATACCCAACCTTTTGTCCGTAGAGATATCATAACATGGCTGAAGTGGCTA

Library


Library category	ESTs
melon 46d library	1
melon PSI library	1

Homology

[Close]

BLAST of MU52206 vs. Swiss-Prot
Match: AMY2_ARATH (Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 4.5e-80
Identity = 138/181 (76.24%), Postives = 159/181 (87.85%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MG ++NVFDE  ++TD G VIR+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. Swiss-Prot
Match: AMY3_ARATH (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.0e-36
Identity = 77/163 (47.24%), Postives = 102/163 (62.58%), Query Frame = 1


Query: 367 NGREILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 426
           +G EIL Q FNWES+K   W   L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 427 LNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAV 486
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L WD+ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 487 TSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKWL 546
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWM 654

Query: 547  606
           
Sbjct: 673  654

Query: 607  666
           
Sbjct: 733  654

Query: 667  726
           
Sbjct: 793  654

Query: 727  786
           
Sbjct: 853  654

Query: 787  844
           
Sbjct: 913  654

[Close]

BLAST of MU52206 vs. Swiss-Prot
Match: AMY3_WHEAT (Alpha-amylase AMY3 OS=Triticum aestivum GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.2e-30
Identity = 71/166 (42.77%), Postives = 93/166 (56.02%), Query Frame = 1


Query: 376 EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 435
           +ILFQ FNWES K    W   ++ KV +IA +G T  WLPPP+ S++P+GYLP  LY+LN
Sbjct: 26  QILFQGFNWESWKTQGGWYKFMQGKVEEIASTGATHVWLPPPSQSVSPEGYLPGQLYNLN 85

Query: 436 SSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDEH 495
           S YGS   LK+L+   +   +  +ADIVINHR        G++  F+G      L W   
Sbjct: 86  SKYGSGADLKSLIQAFRGKNISCVADIVINHRCADKKDGRGVYCIFEGGTSDNRLDWGPD 145

Query: 496 AV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKWL 555
            +    T  S G G+R TG  F   P+IDH  P V+R++  WL WL
Sbjct: 146 EICSDDTKYSNGRGHRDTGGGFDAAPDIDHLNPRVQRELSAWLNWL 191

Query: 556  615
           
Sbjct: 206  191

Query: 616  675
           
Sbjct: 266  191

Query: 676  735
           
Sbjct: 326  191

Query: 736  795
           
Sbjct: 386  191

Query: 796  844
           
Sbjct: 446  191

[Close]

BLAST of MU52206 vs. Swiss-Prot
Match: AMY3D_ORYSJ (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 5.5e-30
Identity = 72/167 (43.11%), Postives = 96/167 (57.49%), Query Frame = 1


Query: 376 EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 435
           ++LFQ FNWES K    W   L+ +V DIAK+G T  WLPPP+HS+APQGY+P  LY L+
Sbjct: 26  QVLFQGFNWESWKQQGGWYNMLKGQVDDIAKAGVTHVWLPPPSHSVAPQGYMPGRLYDLD 85

Query: 436 SS-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDE 495
           +S YG+   LK+L+       V+ +AD+VINHR        G++  F+G      L W  
Sbjct: 86  ASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTPDDRLDWGP 145

Query: 496 HAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKWL 555
             +    T  S G G+R TG+ F   P+IDH  P V+R++  WL WL
Sbjct: 146 GMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWL 192

Query: 556  615
           
Sbjct: 206  192

Query: 616  675
           
Sbjct: 266  192

Query: 676  735
           
Sbjct: 326  192

Query: 736  795
           
Sbjct: 386  192

Query: 796  844
           
Sbjct: 446  192

[Close]

BLAST of MU52206 vs. Swiss-Prot
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 7.2e-30
Identity = 71/167 (42.51%), Postives = 96/167 (57.49%), Query Frame = 1


Query: 376 EILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 435
           +ILFQ FNW+S K    W   L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 436 SS-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDE 495
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 496 HAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKWL 555
             +    T  S G G+R TG +F   P+IDH  P V+R++  WL+WL
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWL 195

Query: 556  615
           
Sbjct: 209  195

Query: 616  675
           
Sbjct: 269  195

Query: 676  735
           
Sbjct: 329  195

Query: 736  795
           
Sbjct: 389  195

Query: 796  844
           
Sbjct: 449  195

[Close]

BLAST of MU52206 vs. TrEMBL
Match: E5GCD3_CUCME (Alpha-amylase OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.4e-104
Identity = 179/181 (98.90%), Postives = 180/181 (99.45%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MGDHDNVFDENYE+TDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDII WLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. TrEMBL
Match: A0A0A0L5R6_CUCSA (Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.7e-97
Identity = 169/181 (93.37%), Postives = 175/181 (96.69%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDII WLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. TrEMBL
Match: D7KTA0_ARALL (Alpha-amylase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.2e-78
Identity = 138/181 (76.24%), Postives = 161/181 (88.95%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MG H+NVFDE  ++TD G V+R+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYHNNVFDECNDQTDIGRVLRDGREVILQAYNWESHKHDWWRNLDGKVPDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. TrEMBL
Match: R0IAQ9_9BRAS (Alpha-amylase OS=Capsella rubella GN=CARUB_v10020375mg PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.2e-77
Identity = 135/181 (74.59%), Postives = 161/181 (88.95%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MG ++NVFD+  ++TD G V+R+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDQCNDQTDIGRVLRDGREVILQAYNWESHKHDWWRNLDGKVPDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLHKMKQYKVRAMADIVINHRVGTTR 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGM+NR+DG+ LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGLSLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQQFVRKDIIGWLRW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. TrEMBL
Match: M5XLS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.2e-77
Identity = 139/181 (76.80%), Postives = 152/181 (83.98%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MG   N  +EN ++TD GA +RNGREILFQAFNWESHK DW  NLE KV D+ +SGFTSA
Sbjct: 1   MGHRGNDSNENSQQTDIGAALRNGREILFQAFNWESHKHDWWRNLERKVPDMGRSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPP THS AP+GYLPQNLYSLNS YGSE +LK LL KMKQ KVR MADIVINHRVGT  
Sbjct: 61  WLPPATHSFAPEGYLPQNLYSLNSKYGSEHLLKGLLQKMKQHKVRPMADIVINHRVGTTQ 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGM+NR+DGI L WDEHAVTSC+GGLGNRSTGDNFHGVPNIDHTQ FVR+DI  WL+W
Sbjct: 121 GHGGMYNRYDGISLSWDEHAVTSCTGGLGNRSTGDNFHGVPNIDHTQLFVRKDITAWLQW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. TAIR10
Match: AT1G76130.1 (AT1G76130.1 alpha-amylase-like 2)

HSP 1 Score: 299.3 bits (765), Expect = 2.5e-81
Identity = 138/181 (76.24%), Postives = 159/181 (87.85%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MG ++NVFDE  ++TD G VIR+GRE++ QA+NWESHK DW  NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRAMADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGM+NR+DGI LPWDEHAVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. TAIR10
Match: AT1G69830.1 (AT1G69830.1 alpha-amylase-like 3)

HSP 1 Score: 155.2 bits (391), Expect = 5.9e-38
Identity = 77/163 (47.24%), Postives = 102/163 (62.58%), Query Frame = 1


Query: 367 NGREILFQAFNWESHKCD-WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 426
           +G EIL Q FNWES+K   W   L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 427 LNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAV 486
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L WD+ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 487 TSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKWL 546
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWM 654

Query: 547  606
           
Sbjct: 673  654

Query: 607  666
           
Sbjct: 733  654

Query: 667  726
           
Sbjct: 793  654

Query: 727  786
           
Sbjct: 853  654

Query: 787  844
           
Sbjct: 913  654

[Close]

BLAST of MU52206 vs. TAIR10
Match: AT4G25000.1 (AT4G25000.1 alpha-amylase-like)

HSP 1 Score: 129.0 bits (323), Expect = 4.5e-30
Identity = 72/165 (43.64%), Postives = 95/165 (57.58%), Query Frame = 1


Query: 379 ILFQAFNWESHKCD--WSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 438
           +LFQ+FNWES K +  +  +L   + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 439 S-YGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDG----ILLPWDEH 498
           S YGSE  LK+L+  + Q  ++A+ADIVINHR          +  F+G      L WD  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 499 AVTSCS---GGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKWL 558
            V        G GN  TG +F G P+IDH  P V++++  W+ WL
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWL 191

Query: 559  618
           
Sbjct: 207  191

Query: 619  678
           
Sbjct: 267  191

Query: 679  738
           
Sbjct: 327  191

Query: 739  798
           
Sbjct: 387  191

Query: 799  844
           
Sbjct: 447  191

[Close]

BLAST of MU52206 vs. NCBI nr
Match: gi|659104355|ref|XP_008452914.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 438.0 bits (1125), Expect = 1.3e-119
Identity = 200/202 (99.01%), Postives = 201/202 (99.50%), Query Frame = 1


Query: 238 LGRPYLLQPRRQGFHKFLPSIMGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKC 297
           LGRPYLLQPRRQGFHKFLPSIMGDHDNVFDENYE+TDTGAVIRNGREILFQAFNWESHKC
Sbjct: 5   LGRPYLLQPRRQGFHKFLPSIMGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKC 64

Query: 298 DWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKM 357
           DWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKM
Sbjct: 65  DWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKM 124

Query: 358 KQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHG 417
           KQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHG
Sbjct: 125 KQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHG 184

Query: 418 VPNIDHTQPFVRRDIITWLKWL 477
           VPNIDHTQPFVRRDII WLKWL
Sbjct: 185 VPNIDHTQPFVRRDIIAWLKWL 206

Query: 478  537
           
Sbjct: 245  206

Query: 538  597
           
Sbjct: 305  206

Query: 598  657
           
Sbjct: 365  206

Query: 658  717
           
Sbjct: 425  206

Query: 718  777
           
Sbjct: 485  206

Query: 778  837
           
Sbjct: 545  206

Query: 838  844
           
Sbjct: 605  206

[Close]

BLAST of MU52206 vs. NCBI nr
Match: gi|659104371|ref|XP_008452921.1| (PREDICTED: probable alpha-amylase 2 isoform X3 [Cucumis melo])

HSP 1 Score: 438.0 bits (1125), Expect = 1.3e-119
Identity = 200/202 (99.01%), Postives = 201/202 (99.50%), Query Frame = 1


Query: 238 LGRPYLLQPRRQGFHKFLPSIMGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKC 297
           LGRPYLLQPRRQGFHKFLPSIMGDHDNVFDENYE+TDTGAVIRNGREILFQAFNWESHKC
Sbjct: 5   LGRPYLLQPRRQGFHKFLPSIMGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKC 64

Query: 298 DWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKM 357
           DWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKM
Sbjct: 65  DWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKM 124

Query: 358 KQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHG 417
           KQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHG
Sbjct: 125 KQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHG 184

Query: 418 VPNIDHTQPFVRRDIITWLKWL 477
           VPNIDHTQPFVRRDII WLKWL
Sbjct: 185 VPNIDHTQPFVRRDIIAWLKWL 206

Query: 478  537
           
Sbjct: 245  206

Query: 538  597
           
Sbjct: 305  206

Query: 598  657
           
Sbjct: 365  206

Query: 658  717
           
Sbjct: 425  206

Query: 718  777
           
Sbjct: 485  206

Query: 778  837
           
Sbjct: 545  206

Query: 838  844
           
Sbjct: 605  206

[Close]

BLAST of MU52206 vs. NCBI nr
Match: gi|307136308|gb|ADN34132.1| (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 392.9 bits (1008), Expect = 4.7e-106
Identity = 179/181 (98.90%), Postives = 180/181 (99.45%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MGDHDNVFDENYE+TDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDII WLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. NCBI nr
Match: gi|659104363|ref|XP_008452918.1| (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 392.9 bits (1008), Expect = 4.7e-106
Identity = 179/181 (98.90%), Postives = 180/181 (99.45%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MGDHDNVFDENYE+TDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDII WLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

[Close]

BLAST of MU52206 vs. NCBI nr
Match: gi|778697053|ref|XP_011654246.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 368.2 bits (944), Expect = 1.2e-98
Identity = 169/181 (93.37%), Postives = 175/181 (96.69%), Query Frame = 1


Query: 301 MGDHDNVFDENYERTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 360
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 361 WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 420
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 421 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIITWLKW 480
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDII WLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 481 L 540
           L
Sbjct: 181 L 181

Query: 541  600
           
Sbjct: 241  181

Query: 601  660
           
Sbjct: 301  181

Query: 661  720
           
Sbjct: 361  181

Query: 721  780
           
Sbjct: 421  181

Query: 781  840
           
Sbjct: 481  181

Query: 841  844
           
Sbjct: 541  181

The following BLAST results are available for this feature:

BLAST of MU52206 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AMY2_ARATH	4.5e-80	76.24	Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1	[more]
AMY3_ARATH	1.0e-36	47.24	Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1	[more]
AMY3_WHEAT	4.2e-30	42.77	Alpha-amylase AMY3 OS=Triticum aestivum GN=AMY1.1 PE=2 SV=1	[more]
AMY3D_ORYSJ	5.5e-30	43.11	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica GN=AMY1.3 PE=2 SV=2	[more]
AMY3A_ORYSJ	7.2e-30	42.51	Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2	[more]

BLAST of MU52206 vs. TrEMBL


Match Name	E-value	Identity	Description
E5GCD3_CUCME	1.4e-104	98.90	Alpha-amylase OS=Cucumis melo subsp. melo PE=3 SV=1	[more]
A0A0A0L5R6_CUCSA	3.7e-97	93.37	Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1	[more]
D7KTA0_ARALL	2.2e-78	76.24	Alpha-amylase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=3 SV=1	[more]
R0IAQ9_9BRAS	4.2e-77	74.59	Alpha-amylase OS=Capsella rubella GN=CARUB_v10020375mg PE=3 SV=1	[more]
M5XLS5_PRUPE	7.2e-77	76.80	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1	[more]

BLAST of MU52206 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G76130.1	2.5e-81	76.24	alpha-amylase-like 2	[more]
AT1G69830.1	5.9e-38	47.24	alpha-amylase-like 3	[more]
AT4G25000.1	4.5e-30	43.64	alpha-amylase-like	[more]

BLAST of MU52206 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659104355\|ref\|XP_008452914.1\|	1.3e-119	99.01	PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo]	[more]
gi\|659104371\|ref\|XP_008452921.1\|	1.3e-119	99.01	PREDICTED: probable alpha-amylase 2 isoform X3 [Cucumis melo]	[more]
gi\|307136308\|gb\|ADN34132.1\|	4.7e-106	98.90	cytosolic alpha-amylase [Cucumis melo subsp. melo]	[more]
gi\|659104363\|ref\|XP_008452918.1\|	4.7e-106	98.90	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo]	[more]
gi\|778697053\|ref\|XP_011654246.1\|	1.2e-98	93.37	PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR006047	Glyco_hydro_13_cat_dom
IPR013781	Glycoside hydrolase, catalytic domain
IPR015902	Glycoside hydrolase, family 13
IPR017853	Glycoside_hydrolase_SF

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0003824	catalytic activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
MELO3C017002	MELO3C017002	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
46D34C06	46D34C06	EST
PSI07C03	PSI07C03	EST

InterPro

Analysis Name: InterPro Annotations of melon unigene v4
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 51..114 score: 3.
IPR013781	Glycoside hydrolase, catalytic domain	GENE3D	G3DSA:3.20.20.80		coord: 23..181 score: 1.5
IPR015902	Glycoside hydrolase, family 13	PANTHER	PTHR10357	ALPHA-AMYLASE	coord: 26..181 score: 5.8E
IPR017853	Glycoside hydrolase superfamily	unknown	SSF51445	(Trans)glycosidases	coord: 40..181 score: 1.7
None	No IPR available	PANTHER	PTHR10357:SF118	ALPHA-AMYLASE 2-RELATED	coord: 26..181 score: 5.8E