Cucsa.271900 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.271900 vs. Swiss-Prot
Match: AMY2_ARATH (Probable alpha-amylase 2 OS=Arabidopsis thaliana GN=AMY2 PE=2 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 2.0e-206
Identity = 319/413 (77.24%), Postives = 375/413 (90.80%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MG ++NVFDE  ++TD G VIR+GRE++ QA++WESHKYDWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI A KP+ S+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDH Y+WG S+HD+IV+LIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRS+V++L+A S+LY+AI+GEK+CMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of Cucsa.271900 vs. Swiss-Prot
Match: AMY3_ARATH (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana GN=AMY3 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-114
Identity = 196/397 (49.37%), Postives = 270/397 (68.01%), Query Frame = 1

Query: 23  NGREILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q F+WES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCSG---GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A KP  ++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAVRR-QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH++      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGEKLCMKIGDGSW-CPSGTE-WTLATSGWRYAVWQ 413
           II EK+ MKIG G +  P+G++ W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of Cucsa.271900 vs. Swiss-Prot
Match: AMY3E_ORYSJ (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 4.4e-100
Identity = 183/404 (45.30%), Postives = 255/404 (63.12%), Query Frame = 1

Query: 26  EILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           ++LFQ F+WES +    W+  L  KV +IA +G T  WLPPP+HS++PQGY+P  LY L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 86  SS-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIL----LPWNE 145
           +S YG+E  LK+L+       V  +ADIVINHR        G++  F+G      L W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 146 YAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG  F   P+IDH  P V+R++  WL WLR+ +GF  +R DF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 206 ARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDL-DYNQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++   P   +GE W S  YNG      NQD  RQ ++NW++G G+ + 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 266 AFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+ +LWRL D  GK PG+MGW P +AVTF+DNHDTGSTQ+ WPFP++
Sbjct: 266 AFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSD 325

Query: 326 HIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSD 385
            +M+GYAYILTHPGIP +FYDH+++W  ++  EI  L +IR    I++ S++ IL+A  D
Sbjct: 326 KVMQGYAYILTHPGIPCIFYDHVFDW--NLQHEIATLAEIRSRNGIHAESTLDILKAEGD 385

Query: 386 LYSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 414
           +Y A+I  K+  K+G     G   PS  ++ +   G  Y VW+K
Sbjct: 386 IYVAMIDGKVITKLGPRYDAGGIIPS--DFHVVAHGNDYCVWEK 425

BLAST of Cucsa.271900 vs. Swiss-Prot
Match: AMYA_VIGMU (Alpha-amylase OS=Vigna mungo GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 5.7e-100
Identity = 181/402 (45.02%), Postives = 260/402 (64.68%), Query Frame = 1

Query: 27  ILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNSS 86
           +LFQ F+WES K   W+ +L++ + D+A +G T  WLPPP+ S++P+GYLP  LY L++S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 87  -YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDG----ILLPWNEYA 146
            YGS++ LK+L+    +  ++ +ADIVINHR        G++  F+G        W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 147 V----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
           +    T+ S G GN  +G+ +   P+IDH  P V+R++  W+ WL++ +GF  +RFDF +
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 207 GFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHD--LDYNQDGHRQRIINWIDGTGQLSAA 266
           G++    K Y+E  KP  ++GE WDS +Y G D   +YNQD HR  ++NW++  G    A
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVNWVESAGGAITA 264

Query: 267 FDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AV+ +LWRL D  GKPPG++G  P  AVTF+DNHDTGSTQ  WPFP++ 
Sbjct: 265 FDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 327 IMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSDL 386
           +M+GYAYILTHPG P++FYDH ++WG  + ++I +L  IR    IN +S+VKI+ +  DL
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNGINEKSTVKIMASEGDL 384

Query: 387 YSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQ 413
           Y A I  K+ +KIG     G+  PS     +ATSG  YAVW+
Sbjct: 385 YVAKIDNKIMVKIGPKMDLGNLIPSNLH--VATSGQDYAVWE 421

BLAST of Cucsa.271900 vs. Swiss-Prot
Match: AMY3A_ORYSJ (Alpha-amylase isozyme 3A OS=Oryza sativa subsp. japonica GN=AMY1.2 PE=2 SV=2)

HSP 1 Score: 365.5 bits (937), Expect = 7.5e-100
Identity = 180/404 (44.55%), Postives = 258/404 (63.86%), Query Frame = 1

Query: 26  EILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLN 85
           +ILFQ F+W+S K    W+  L+ +V DIA +G T  WLPPPTHS++PQGY+P  LY LN
Sbjct: 29  QILFQGFNWDSWKKQGGWYNMLKDQVGDIASAGVTHVWLPPPTHSVSPQGYMPGRLYDLN 88

Query: 86  SS-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGI----LLPWNE 145
           +S YG++  LK+L+       ++ +ADIV+NHR        G++  F G      L W  
Sbjct: 89  ASKYGTKAELKSLIAAFHAKGIKCVADIVVNHRCADDKDGRGVYCIFKGGGPRGCLDWGP 148

Query: 146 YAV----TSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDF 205
             +    T  S G G+R TG +F   P+IDH  P V+R++  WL+WLR  VGF  +R DF
Sbjct: 149 SMICCDDTQYSDGTGHRDTGADFAAAPDIDHLNPLVQRELSDWLRWLRRDVGFDGWRLDF 208

Query: 206 ARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDY-NQDGHRQRIINWIDGTGQLSA 265
           A+G+SA   + Y++  +P   + E W+S +Y+G      NQDG RQ ++NW+   G  + 
Sbjct: 209 AKGYSAAVARTYVQNARPSFVVAEIWNSLSYDGDGKPAANQDGQRQELVNWVKQVGGPAT 268

Query: 266 AFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPAN 325
           AFDFTTKG+LQ AV+ +LWR+RD  GK PG++GW+P +AVTF+DNHDTGSTQ  WPFP++
Sbjct: 269 AFDFTTKGILQSAVQGELWRMRDKDGKAPGMIGWYPEKAVTFVDNHDTGSTQRMWPFPSD 328

Query: 326 HIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSD 385
            ++ GYAYILTHPG+P +FYDH+++W  ++  EI  L   RK   IN+ S +++L A SD
Sbjct: 329 KVILGYAYILTHPGVPCIFYDHVFDW--NLKQEINALAATRKRNGINAGSKLRVLAAESD 388

Query: 386 LYSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 414
           +Y A++ E++  KIG     G+  PS  ++ +   G  Y VW+K
Sbjct: 389 MYVAMVDERVITKIGPRIDVGNIIPS--DFHIVAHGNDYCVWEK 428

BLAST of Cucsa.271900 vs. TrEMBL
Match: A0A0A0L5R6_CUCSA (Alpha-amylase OS=Cucumis sativus GN=Csa_4G652880 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 2.5e-251
Identity = 410/413 (99.27%), Postives = 410/413 (99.27%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI   IDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

BLAST of Cucsa.271900 vs. TrEMBL
Match: E5GCD3_CUCME (Alpha-amylase OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 6.5e-236
Identity = 389/417 (93.29%), Postives = 399/417 (95.68%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIV+LIDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416

BLAST of Cucsa.271900 vs. TrEMBL
Match: M5XLS5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa006402mg PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.4e-206
Identity = 328/413 (79.42%), Postives = 370/413 (89.59%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MG   N  +E+ ++TD GA +RNGREILFQAF+WESHK+DWW NLE KV D+ +SGFTSA
Sbjct: 1   MGHRGNDSNENSQQTDIGAALRNGREILFQAFNWESHKHDWWRNLERKVPDMGRSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPP THS AP+GYLPQNLYSLNS YGSE +LK LL KMKQ KVR +ADIVINHRVGT  
Sbjct: 61  WLPPATHSFAPEGYLPQNLYSLNSKYGSEHLLKGLLQKMKQHKVRPMADIVINHRVGTTQ 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI L W+E+AVTSC+GGLGNRSTGDNFHGVPNIDHTQ FVR+DI AWL+W
Sbjct: 121 GHGGMYNRYDGISLSWDEHAVTSCTGGLGNRSTGDNFHGVPNIDHTQLFVRKDITAWLQW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SAK+VKEYIE  KP+ S+GEYWDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNNVGFQDFRFDFARGYSAKYVKEYIEGAKPIFSVGEYWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDG GQLS AFDFTTKG+LQEAV+ QLWRLRD QGKPPGV+GWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGAGQLSTAFDFTTKGILQEAVKGQLWRLRDPQGKPPGVIGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFP+NHIMEGYAYILTHPGIP+VFYDH Y+W DS+HD+IV+LIDIRKHQDI+
Sbjct: 301 TGSTQAHWPFPSNHIMEGYAYILTHPGIPSVFYDHFYDWSDSIHDQIVKLIDIRKHQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRSS++ILEA+ +LYSAIIGEK+CMKIGD SWCP+G EWTLAT G RYA+W K
Sbjct: 361 SRSSIRILEAQPNLYSAIIGEKVCMKIGDSSWCPTGREWTLATCGHRYAIWHK 413

BLAST of Cucsa.271900 vs. TrEMBL
Match: A0A067HB02_CITSI (Alpha-amylase OS=Citrus sinensis GN=CISIN_1g014892mg PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 5.4e-206
Identity = 327/406 (80.54%), Postives = 370/406 (91.13%), Query Frame = 1

Query: 8   FDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPTH 67
           FDE+ ++TD GAVIRNGREILFQ F+WES K+DWW NLE KV DI+KSGFTS WLPP TH
Sbjct: 8   FDETNQQTDLGAVIRNGREILFQGFNWESCKHDWWRNLERKVPDISKSGFTSVWLPPATH 67

Query: 68  SIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHN 127
           S AP+GYLPQNLYSLNSSYGSE +LK LL KMKQ KVRA+ADIVINHRVGT  GHGG +N
Sbjct: 68  SFAPEGYLPQNLYSLNSSYGSEHLLKALLHKMKQHKVRAMADIVINHRVGTTQGHGGKYN 127

Query: 128 RFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGF 187
           R+DGI L W+E+AVTSC+GGLGN STGDNFHGVPNIDHTQ FVR+DIIAWL+WLR++VGF
Sbjct: 128 RYDGIPLSWDEHAVTSCTGGLGNGSTGDNFHGVPNIDHTQHFVRKDIIAWLRWLRNTVGF 187

Query: 188 QDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDG 247
           QDFRFDFARG+SAK+VKEYIE  +P+ S+GEYWDSCNYN H LDYNQD HRQRIINWIDG
Sbjct: 188 QDFRFDFARGYSAKYVKEYIEGARPIFSVGEYWDSCNYNSHGLDYNQDSHRQRIINWIDG 247

Query: 248 TGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAH 307
           TGQLSAAFDFTTKG+LQEAV+ Q WRLRD QGKPPGVMGWWPSRAVTFLDNHDTGSTQAH
Sbjct: 248 TGQLSAAFDFTTKGILQEAVKGQFWRLRDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAH 307

Query: 308 WPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKI 367
           WPFP+NHIMEGYAYIL HPGIP+VFYDH Y+WGDS+H++IV+L+D+R+ QDI+SRSS+KI
Sbjct: 308 WPFPSNHIMEGYAYILMHPGIPSVFYDHFYDWGDSIHNQIVKLMDVRRQQDIHSRSSIKI 367

Query: 368 LEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           LEA+S+LYSAIIG+K+CMKIGDGSWCP+G EWTLATSG +YAVW K
Sbjct: 368 LEAQSNLYSAIIGDKVCMKIGDGSWCPAGKEWTLATSGHKYAVWHK 413

BLAST of Cucsa.271900 vs. TrEMBL
Match: D7KTA0_ARALL (Alpha-amylase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_316534 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 2.3e-204
Identity = 319/413 (77.24%), Postives = 375/413 (90.80%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MG H+NVFDE  ++TD G V+R+GRE++ QA++WESHK+DWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYHNNVFDECNDQTDIGRVLRDGREVILQAYNWESHKHDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI A KP+ S+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFP++HIMEGYAYILTHPGIP VFYDH Y+WG S+HD+IV+LIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHIMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRS++++L+A S+LY+AI+GEKLCMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 361 SRSTIRVLKAESNLYAAIVGEKLCMKLGDGSWCPSGGDWTLATSGHRYAVWHK 413

BLAST of Cucsa.271900 vs. TAIR10
Match: AT1G76130.1 (AT1G76130.1 alpha-amylase-like 2)

HSP 1 Score: 719.5 bits (1856), Expect = 1.1e-207
Identity = 319/413 (77.24%), Postives = 375/413 (90.80%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MG ++NVFDE  ++TD G VIR+GRE++ QA++WESHKYDWW NL+ KV DIAKSGFTSA
Sbjct: 1   MGYYNNVFDECNDQTDIGRVIRDGREVILQAYNWESHKYDWWRNLDGKVPDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPP+ S+AP+GYLPQ+LYSLNS+YGSE +LK+LL KMKQ KVRA+ADIVINHRVGT  
Sbjct: 61  WLPPPSQSLAPEGYLPQDLYSLNSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTR 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGM+NR+DGI LPW+E+AVTSC+GGLGNRSTGDNF+GVPN+DHTQ FVR+DII WL+W
Sbjct: 121 GHGGMYNRYDGISLPWDEHAVTSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LR++VGFQDFRFDFARG+SA +VKEYI A KP+ S+GE WDSCNYNGH LDYNQD HRQR
Sbjct: 181 LRNTVGFQDFRFDFARGYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           II+WID TGQ+SAAFDFTTKG+LQEAV+ Q WRL D QGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IISWIDATGQISAAFDFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFP++H+MEGYAYILTHPGIP VFYDH Y+WG S+HD+IV+LIDIR+ QDI+
Sbjct: 301 TGSTQAHWPFPSHHVMEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIH 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRS+V++L+A S+LY+AI+GEK+CMK+GDGSWCPSG +WTLATSG RYAVW K
Sbjct: 361 SRSTVRVLKAESNLYAAIVGEKICMKLGDGSWCPSGRDWTLATSGHRYAVWHK 413

BLAST of Cucsa.271900 vs. TAIR10
Match: AT1G69830.1 (AT1G69830.1 alpha-amylase-like 3)

HSP 1 Score: 414.1 bits (1063), Expect = 1.0e-115
Identity = 196/397 (49.37%), Postives = 270/397 (68.01%), Query Frame = 1

Query: 23  NGREILFQAFSWESHKYD-WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYS 82
           +G EIL Q F+WES+K   W+  L+ K  ++A  GFT  WLPPPT S++P+GY+P++LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 83  LNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAV 142
           LNS YG+ D LK  + K  +  ++ + D V+NHR        G+ N F G  L W++ AV
Sbjct: 553 LNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAV 612

Query: 143 TSCSG---GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGF 202
            +      G GN+S+GDNFH  PNIDH+Q FVR+DI  WL W+   VG+  +R DF RGF
Sbjct: 613 VADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGF 672

Query: 203 SAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFT 262
              +VK+Y++A KP  ++GEYWDS +Y   ++DYNQD HRQRI++WI+ T   + AFD T
Sbjct: 673 WGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVT 732

Query: 263 TKGVLQEAVRR-QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 322
           TKG+L  A+++ + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+
Sbjct: 733 TKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQ 792

Query: 323 GYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSDLYSA 382
           GYAYILTHPG P VF+DH++      H EI  L+ +R  Q ++ RS V I ++  D+Y+A
Sbjct: 793 GYAYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAA 852

Query: 383 IIGEKLCMKIGDGSW-CPSGTE-WTLATSGWRYAVWQ 413
           II EK+ MKIG G +  P+G++ W++A  G  Y VW+
Sbjct: 853 IIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

BLAST of Cucsa.271900 vs. TAIR10
Match: AT4G25000.1 (AT4G25000.1 alpha-amylase-like)

HSP 1 Score: 340.9 bits (873), Expect = 1.1e-93
Identity = 181/403 (44.91%), Postives = 252/403 (62.53%), Query Frame = 1

Query: 27  ILFQAFSWESHKYD--WWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSLNS 86
           +LFQ+F+WES K +  ++ +L + + DIA +G T  WLPPP+ S+AP+GYLP  LY LNS
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 87  S-YGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGIL----LPWNEY 146
           S YGSE  LK+L+  + Q  ++A+ADIVINHR          +  F+G      L W+  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 147 AVTSCSG---GLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFAR 206
            V        G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+ R
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 207 GFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGH-DLDYNQDGHRQRIINWI-DGTGQLSAA 266
           G+++   K Y++   P  ++GE WD   Y G   LDY+Q+ HR  +  WI +  G +  A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 267 FDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 326
           FDFTTKG+LQ AV+ +LWRL+D QGKPPG++G  P  AVTF+DNHDT  T   W FP++ 
Sbjct: 267 FDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDK 326

Query: 327 IMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSDL 386
           ++ GY YILTHPG P +FY+H  EWG  + + I +L+ IR    I S SSV I  A +DL
Sbjct: 327 VLLGYVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEADL 386

Query: 387 YSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGWRYAVWQK 414
           Y A+I +K+ MKIG     G+  PS   + LA SG  +AVW+K
Sbjct: 387 YLAMIDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422

BLAST of Cucsa.271900 vs. NCBI nr
Match: gi|778697053|ref|XP_011654246.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 885.9 bits (2288), Expect = 2.6e-254
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 413

BLAST of Cucsa.271900 vs. NCBI nr
Match: gi|778697076|ref|XP_011654253.1| (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 875.5 bits (2261), Expect = 3.5e-251
Identity = 410/413 (99.27%), Postives = 410/413 (99.27%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI   IDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEI---IDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 410

BLAST of Cucsa.271900 vs. NCBI nr
Match: gi|659104355|ref|XP_008452914.1| (PREDICTED: probable alpha-amylase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 830.1 bits (2143), Expect = 1.7e-237
Identity = 389/413 (94.19%), Postives = 399/413 (96.61%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 26  MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 85

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 86  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 145

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 146 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 205

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 206 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 265

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 266 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 325

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIV+LIDIRKHQDIN
Sbjct: 326 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 385

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 386 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

BLAST of Cucsa.271900 vs. NCBI nr
Match: gi|659104363|ref|XP_008452918.1| (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 830.1 bits (2143), Expect = 1.7e-237
Identity = 389/413 (94.19%), Postives = 399/413 (96.61%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQR 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQDGHRQR
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQR 240

Query: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHD 300
           IINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFLDNHD
Sbjct: 241 IINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHD 300

Query: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDIN 360
           TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIV+LIDIRKHQDIN
Sbjct: 301 TGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDIN 360

Query: 361 SRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           SRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 SRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 412

BLAST of Cucsa.271900 vs. NCBI nr
Match: gi|307136308|gb|ADN34132.1| (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 824.3 bits (2128), Expect = 9.4e-236
Identity = 389/417 (93.29%), Postives = 399/417 (95.68%), Query Frame = 1

Query: 1   MGDHDNVFDESYEKTDTGAVIRNGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSA 60
           MGDHDNVFDE+YE+TDTGAVIRNGREILFQAF+WESHK DW  NLE KVHDIAKSGFTSA
Sbjct: 1   MGDHDNVFDENYEQTDTGAVIRNGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSA 60

Query: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVV 120
           WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLK LLLKMKQCKVRA+ADIVINHRVGTVV
Sbjct: 61  WLPPPTHSIAPQGYLPQNLYSLNSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVV 120

Query: 121 GHGGMHNRFDGILLPWNEYAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180
           GHGGMHNRFDGILLPW+E+AVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW
Sbjct: 121 GHGGMHNRFDGILLPWDEHAVTSCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKW 180

Query: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQ----DG 240
           LRSSVGFQDFRFDFARGFSAKFVKEYIEA KPM SIGEYWDSCNYNGHDLDYNQ    DG
Sbjct: 181 LRSSVGFQDFRFDFARGFSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADG 240

Query: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFL 300
           HRQRIINWIDGTGQLSAAFDFTTKGVLQEAV+ QLWRL DC+GKPPGVMGWWPSRAVTFL
Sbjct: 241 HRQRIINWIDGTGQLSAAFDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFL 300

Query: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKH 360
           DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDH Y+WGDSVHDEIV+LIDIRKH
Sbjct: 301 DNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKH 360

Query: 361 QDINSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 414
           QDINSRSSVKILEARSDLYSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 361 QDINSRSSVKILEARSDLYSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416