MELO3C005195 (gene) Melon (DHL92) v3.5.1

NameMELO3C005195
Typegene
OrganismCucumis melo (Melon (DHL92) v3.5.1)
DescriptionPectinesterase
Locationchr9 : 17222592 .. 17224688 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGGGAAGGTAATCGTGTCGGCATTATCCCTTATTCTCGTTGTTGGTATAGCTCTAGCCGTTGTCGCCACCATAAACAAAAGGAATGGTTCTAATGGTGGAAAGGAGGAAGTATCACCAAAGATGAAGGCAATCTCAGCCATTTGTTCTACTACAGATTATCAAGAAGAATGCAAAAATACACTTAACCATGTTGCTCAGAATACCTCTTCAAATGACCCCAAAGACTATGCTAAAGCAGCAATTCTTGCCACTATCAAGGAAATTACAAAAGGCTACAACTTGTCTGATAGCCTCATAGTGGAGGCTGGTAACAATGCCTCCATCAAAATGAGTGTTGAAGATTGCAACGATTTGTTACAATTCGCCATTGATGAGTTGCAAGCATCGTATTCCACCGTTGGAGAGTCTGATTTGCATACCGACGCAGATCGTATTGCTGATATTAAGAATTGGCTTAGTGCTGTTGTTTCTTATCAACAATCTTGTCTTGATGGACTTGGAGAATTTGATCCTCAGCTTAAACAGAGGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATTCTCACTGCAGTTTCCAATATCCTTGACAACTACGGCTTGCAATTGAAGGTAAATATGCTAAATCATCATTTATTAGAAAGCTCTTTTTAACGCCATCCAGTTGGAAGTGAAAGGAAGTCGAGTCATAAACTCCGCTATTTGTTTGGCCCAAGTAATTTGCGGTCCTATTATTAAAATAAACATCAATGTTATACCTTATTAACTTCTTACATCATAGAAACCATGAGTTTACAATTAATCCACTCCTTACCTCAAACAACCTCGTAGAAAGTTTAAACTAATGGGATATGACATATTTAATAAGTGTGGTTTGTTTTTTTTAATTAGCAGGTTCAACCAAGTGGTCGTAGGCTTCTTGGAACAACAGAAGTAGATAGCGACGGGTTCCCGACATGGTTGACGGGGGCAGATAGGAAGTTGCTTGCATCAAAACAAGGAGGCGTTCGACCAACACCGAACGCAGTGGTGGCTAAGGATGGTAGTGGGCAATACAAGACCATTGCTGCTGCATTAGCTGCATACCCAAAGGCTCTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATGATGAATACATCACTGTAACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGTCCAAGAAAAACCATTGTCACCGGTCGCAAGAGTAACCGCGACGGTTTCACCACACAAAACACTGCCTCTTTCGGTAAATCACTCCCTACTCAAATTTTTCACAACAATATTTCGTCCTAAATTTTCAAACTATCATAAGATATACAAACTAACTCATTTACATTTCGAAATTTACCTCTTGCAGCTGCCATAGGGGAAGGATTCCTTTGCAAATCCATGGGATTCACCAACACAGCTGGTCCTCAAGGCCACCAAGCGGTAGCCTTACGAGTCCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGTCATCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGAAACTGTGTCATATCAGGTACCGTAGACTTCATCTTCGGTGATTCATCCACCATCATCCAAAACTCCTTAATCATCGTTCGTCGCCCAATGGACAACCAACAAAACACCGTCACAGCCCAAGGTCGCGCCGAGCAAAAAGAAATCACTGGTCTAGTGATCCACAACTGCAGAATTGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCAAGAACTGTGATCATGGAGTCTACATTGGGTGACTTCATTCAGCCAGCAGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACTTGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGGAGAGTGAGATGGAAGGGCGTTAAAGTGATCGGAAGAAATGAAGCTTTGCAATATACTGCTGGTGCATTTCTTCTTGGAAGAACGTGGCTTCCAAGAACTGGTGGGATTTACTACTTGGGATTGAAGAACTGA

mRNA sequence

ATGGTCGGGAAGGTAATCGTGTCGGCATTATCCCTTATTCTCGTTGTTGGTATAGCTCTAGCCGTTGTCGCCACCATAAACAAAAGGAATGGTTCTAATGGTGGAAAGGAGGAAGTATCACCAAAGATGAAGGCAATCTCAGCCATTTGTTCTACTACAGATTATCAAGAAGAATGCAAAAATACACTTAACCATGTTGCTCAGAATACCTCTTCAAATGACCCCAAAGACTATGCTAAAGCAGCAATTCTTGCCACTATCAAGGAAATTACAAAAGGCTACAACTTGTCTGATAGCCTCATAGTGGAGGCTGGTAACAATGCCTCCATCAAAATGAGTGTTGAAGATTGCAACGATTTGTTACAATTCGCCATTGATGAGTTGCAAGCATCGTATTCCACCGTTGGAGAGTCTGATTTGCATACCGACGCAGATCGTATTGCTGATATTAAGAATTGGCTTAGTGCTGTTGTTTCTTATCAACAATCTTGTCTTGATGGACTTGGAGAATTTGATCCTCAGCTTAAACAGAGGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATTCTCACTGCAGTTTCCAATATCCTTGACAACTACGGCTTGCAATTGAAGGTTCAACCAAGTGGTCGTAGGCTTCTTGGAACAACAGAAGTAGATAGCGACGGGTTCCCGACATGGTTGACGGGGGCAGATAGGAAGTTGCTTGCATCAAAACAAGGAGGCGTTCGACCAACACCGAACGCAGTGGTGGCTAAGGATGGTAGTGGGCAATACAAGACCATTGCTGCTGCATTAGCTGCATACCCAAAGGCTCTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATGATGAATACATCACTGTAACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGTCCAAGAAAAACCATTGTCACCGGTCGCAAGAGTAACCGCGACGGTTTCACCACACAAAACACTGCCTCTTTCGCTGCCATAGGGGAAGGATTCCTTTGCAAATCCATGGGATTCACCAACACAGCTGGTCCTCAAGGCCACCAAGCGGTAGCCTTACGAGTCCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGTCATCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGAAACTGTGTCATATCAGGTACCGTAGACTTCATCTTCGGTGATTCATCCACCATCATCCAAAACTCCTTAATCATCGTTCGTCGCCCAATGGACAACCAACAAAACACCGTCACAGCCCAAGGTCGCGCCGAGCAAAAAGAAATCACTGGTCTAGTGATCCACAACTGCAGAATTGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCAAGAACTGTGATCATGGAGTCTACATTGGGTGACTTCATTCAGCCAGCAGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACTTGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGGAGAGTGAGATGGAAGGGCGTTAAAGTGATCGGAAGAAATGAAGCTTTGCAATATACTGCTGGTGCATTTCTTCTTGGAAGAACGTGGCTTCCAAGAACTGGTGGGATTTACTACTTGGGATTGAAGAACTGA

Coding sequence (CDS)

ATGGTCGGGAAGGTAATCGTGTCGGCATTATCCCTTATTCTCGTTGTTGGTATAGCTCTAGCCGTTGTCGCCACCATAAACAAAAGGAATGGTTCTAATGGTGGAAAGGAGGAAGTATCACCAAAGATGAAGGCAATCTCAGCCATTTGTTCTACTACAGATTATCAAGAAGAATGCAAAAATACACTTAACCATGTTGCTCAGAATACCTCTTCAAATGACCCCAAAGACTATGCTAAAGCAGCAATTCTTGCCACTATCAAGGAAATTACAAAAGGCTACAACTTGTCTGATAGCCTCATAGTGGAGGCTGGTAACAATGCCTCCATCAAAATGAGTGTTGAAGATTGCAACGATTTGTTACAATTCGCCATTGATGAGTTGCAAGCATCGTATTCCACCGTTGGAGAGTCTGATTTGCATACCGACGCAGATCGTATTGCTGATATTAAGAATTGGCTTAGTGCTGTTGTTTCTTATCAACAATCTTGTCTTGATGGACTTGGAGAATTTGATCCTCAGCTTAAACAGAGGATGCAAGATGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATTCTCACTGCAGTTTCCAATATCCTTGACAACTACGGCTTGCAATTGAAGGTTCAACCAAGTGGTCGTAGGCTTCTTGGAACAACAGAAGTAGATAGCGACGGGTTCCCGACATGGTTGACGGGGGCAGATAGGAAGTTGCTTGCATCAAAACAAGGAGGCGTTCGACCAACACCGAACGCAGTGGTGGCTAAGGATGGTAGTGGGCAATACAAGACCATTGCTGCTGCATTAGCTGCATACCCAAAGGCTCTAAGAGGAAGATATGTAATCTACGTGAAAGCAGGGATCTATGATGAATACATCACTGTAACTAAAGACATGAAAAATGTTTTCATGTATGGAGATGGTCCAAGAAAAACCATTGTCACCGGTCGCAAGAGTAACCGCGACGGTTTCACCACACAAAACACTGCCTCTTTCGCTGCCATAGGGGAAGGATTCCTTTGCAAATCCATGGGATTCACCAACACAGCTGGTCCTCAAGGCCACCAAGCGGTAGCCTTACGAGTCCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGTCATCAAGACACTCTCTACGTACAAACTCACCGTCAATTCTACCGAAACTGTGTCATATCAGGTACCGTAGACTTCATCTTCGGTGATTCATCCACCATCATCCAAAACTCCTTAATCATCGTTCGTCGCCCAATGGACAACCAACAAAACACCGTCACAGCCCAAGGTCGCGCCGAGCAAAAAGAAATCACTGGTCTAGTGATCCACAACTGCAGAATTGTCCCAGAACAGAAGCTATTCGCTGAGAGGTTCAAGATTCCAACATTCTTGGGGCGACCATGGAAGCAATATGCAAGAACTGTGATCATGGAGTCTACATTGGGTGACTTCATTCAGCCAGCAGGATACATGCCTTGGTCTGGAAATTTTGCATTGGAGACTTGTTTGTATTTGGAGTATGGGAACAGAGGACCTGGTGCTAACACCAACAGGAGAGTGAGATGGAAGGGCGTTAAAGTGATCGGAAGAAATGAAGCTTTGCAATATACTGCTGGTGCATTTCTTCTTGGAAGAACGTGGCTTCCAAGAACTGGTGGGATTTACTACTTGGGATTGAAGAACTGA

Protein sequence

MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECKNTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEALQYTAGAFLLGRTWLPRTGGIYYLGLKN*
BLAST of MELO3C005195 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 228/565 (40.35%), Postives = 350/565 (61.95%), Query Frame = 1

Query: 4   KVIVSALSLILVVGIALAVVA--TINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECKN 63
           + IV  +S +L++ + +AV    ++NK +G + GK EV+  +KA+  +C+ TDY++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDLL 123
           TL    +NT+  DP +  K A   T+K+IT     S + I+E   ++  +M+++ C +L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQD 183
            +A+DEL  S+  +G+ + H   + + +++ WLSA +S++++CL+G         + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 GLDVAGKLTSNALAILTAVSNILDNYGLQLKVQP-SGRRLLGTTEVDSDGFPTWLTGADR 243
            L  A +LT N LAI++ +SN +     Q+++   + RRLL      ++GFP+W+    R
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGR 253

Query: 244 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 303
           KLL +        P+ VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y EY+ V 
Sbjct: 254 KLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVN 313

Query: 304 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 363
           K M ++   GDGP KTI++G K+ +DG TT  TA+ A +G  F+ K++GF NTAG   HQ
Sbjct: 314 KTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQ 373

Query: 364 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 423
           AVA+RVQSD S FFNCR DG+QDTLY  +HRQF+R+C ISGT+DF+FGD++ + QN  ++
Sbjct: 374 AVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLL 433

Query: 424 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 483
           VR+P+ NQ   +TA GR + +E TG V   C I  E    A +     +LGRPWK+Y+RT
Sbjct: 434 VRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRT 493

Query: 484 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 543
           +IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + L
Sbjct: 494 IIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDIL 553

Query: 544 QYTAGAFLLGRTWLPRTGGIYYLGL 566
           ++T   ++ G  W+P  G  Y  GL
Sbjct: 554 KFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of MELO3C005195 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.6e-127
Identity = 227/562 (40.39%), Postives = 345/562 (61.39%), Query Frame = 1

Query: 4   KVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECKNTL 63
           + ++ ++S +L++ + +AV   ++     N G EE++  +KAI  +C+ TDY+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  NHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDLLQF 123
              A++TS  DP +  K A  AT+K+I+     S ++I E   +   KM+++ C +L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQDGL 183
           AI EL  S+  +G+ + H   + +  ++ WLSA +S++Q+CLDG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADRKLL 243
             A +LT N LA++T +SN L    +    + + RRLL      S  FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVTKDM 303
            +    V+P  + VVA+DGSGQYKTI  AL   PK     +V+++K GIY EY+ V + M
Sbjct: 255 NAPMSEVKP--DIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVA 363
            ++   GDGP KT+++G KS +DG TT  TA+ A +G+ F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C ISGT+DF+FGD++ + QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA GR + +E TG V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEALQYT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGAFLLGRTWLPRTGGIYYLGL 566
              ++ G  W+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of MELO3C005195 vs. Swiss-Prot
Match: PME4_ARATH (Pectinesterase 4 OS=Arabidopsis thaliana GN=PME4 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 2.1e-121
Identity = 254/601 (42.26%), Postives = 347/601 (57.74%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           M+GKV+VS  S++L+VG+A+ VVA INK   +N     +SP+MKA+  IC +T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDAN-----LSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASI--KMSVEDCN 120
            TL  V     S DP    KA +LAT  E+TK  N +    V  G++ S   K  ++ C 
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQR 180
            +  +A+++L      +GE DL     +I  +K WL  V +YQ  CLD + E D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDVAGKLTSNALAIL-TAVSNI--LDNYGLQLK--------------------VQP 240
           + +G+  +  LT+NA+ I  T VS +  ++N    LK                      P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SGRRLLGTTEVDSDGFPTWLTGADRKLLA--------SKQGGVRPTPNAVVAKDGSGQYK 300
            G       ++D  G PTW++GADRKL+A         + GG R   N VVAKDGSGQ+K
Sbjct: 241 DGPARRLLEDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQFK 300

Query: 301 TIAAALAAYPKALRGRYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGRKS--NR 360
           T+  A+ A P+  RGR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S    
Sbjct: 301 TVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVALS 360

Query: 361 DGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGHQDTL 420
            G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QDTL
Sbjct: 361 RGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTL 420

Query: 421 YVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQKEI-T 480
           YV   RQFYRNCV+SGTVDFIFG S+T+IQN+LI+VR+    Q NTVTA G      +  
Sbjct: 421 YVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKI 480

Query: 481 GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGNFA 540
           G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G   
Sbjct: 481 GIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESF 540

Query: 541 LETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEALQYTAGAFLLGRTWLPRTGGIYYLG 566
            ++C Y+EY NRGPGA  NRRV W  V      E   +TA  +L    W+        +G
Sbjct: 541 HKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTIG 588

BLAST of MELO3C005195 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.1e-120
Identity = 233/558 (41.76%), Postives = 339/558 (60.75%), Query Frame = 1

Query: 6   IVSALSLILVVGIALAVVATINKRNGSNGGK---EEVSPKMKAISAICSTTDYQEECKNT 65
           +++AL +++VV + +        RN S+  K    ++     A+ A+C+ TDY+E C N+
Sbjct: 17  VITALLVLMVVAVGITT-----SRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNS 76

Query: 66  LNHVAQNTSSNDPKDYAKAAILATIKEITKGYN-LSDSLIVEAGNNASIKMSVEDCNDLL 125
           L  +  +  S  P D  K     TI+ I       S  L  +A N+   K ++E C  L+
Sbjct: 77  L--MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLM 136

Query: 126 QFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQD 185
             A D+L+          +    D + D++ WLS  ++YQQ+C+D   E + +L Q MQ 
Sbjct: 137 NDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQK 196

Query: 186 GLDVAGKLTSNALAILTAVSNILDNY---GLQLKVQPSGRRLLGTTEVDSDGFPTWLTGA 245
               + +LTSN LA++T +SN+L  +   G+   +    R+LL       DG P+W+   
Sbjct: 197 IFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA----EDGIPSWVGPN 256

Query: 246 DRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYIT 305
            R+L+A+K GGV+   N VVA DGSGQYKTI  AL A PKA +  +VIY+K G+Y+E + 
Sbjct: 257 TRRLMATK-GGVKA--NVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVD 316

Query: 306 VTKDMKNVFMYGDGPRKTIVTGRKSNRDG-FTTQNTASFAAIGEGFLCKSMGFTNTAGPQ 365
           VTK M +V   GDGP KT +TG  +   G   T  TA+ A  G+ F  K++GF NTAGP+
Sbjct: 317 VTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPE 376

Query: 366 GHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNS 425
           GHQAVALRV +D + F+NC++DG+QDTLYV +HRQF+R+C +SGTVDFIFGD   ++QN 
Sbjct: 377 GHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNC 436

Query: 426 LIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQY 485
            I+VR+PM +Q   +TAQGR++++E TGLV+ NC I  E      +     +LGRPWK++
Sbjct: 437 NIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEF 496

Query: 486 ARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRN 545
           +RT+IM +T+ D I PAG++PW+G+FAL T  Y EY N GPG+N  +RV+W G+K +   
Sbjct: 497 SRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPK 556

Query: 546 EALQYTAGAFLLGRTWLP 556
           +AL++T   FL G  W+P
Sbjct: 557 QALRFTPARFLRGNLWIP 560

BLAST of MELO3C005195 vs. Swiss-Prot
Match: PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.6e-119
Identity = 246/594 (41.41%), Postives = 352/594 (59.26%), Query Frame = 1

Query: 4   KVIVSALSLILV----VGIALAVVA-----------TINKRNGSNGGKEEVSPKM----- 63
           ++I+  +S+++V    +G A A V            T NK   S    E  SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAISAICSTTDYQEECKNTL-NHVAQNTSSNDPKDYAKAAILATIKEITKG 123
                    K I  +C++T Y+  C+NTL N   ++T   DP+   K+AI+A   ++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLSDSLIVEAGNNASIKMSVEDCNDLLQFAIDELQASYSTVGESDLHTDADRIADIKNW 183
           +    SL  E   N   K ++  C  L+  A +EL  S   + +S+++  A  + D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LSAVVSYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKV 243
           LSAV+SYQ++C+DG  E   +LK  ++   + +  LTSN+LA++ ++   L +     KV
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KV 263

Query: 244 QPSGRRLLGTTEVDSDGFPTWLTGADRKLLASKQGGVRPT-PNAVVAKDGSGQYKTIAAA 303
           +        ++  ++D   +WL+  +R++L  K   V+   PNA VAKDGSG + TI AA
Sbjct: 264 KTRLLLEARSSAKETDHITSWLSNKERRML--KAVDVKALKPNATVAKDGSGNFTTINAA 323

Query: 304 LAAYPKALRGRYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQN 363
           L A P   +GRY IY+K GIYDE + + K   NV M GDG +KTIVTG KS+     T  
Sbjct: 324 LKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFL 383

Query: 364 TASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQ 423
           TA+F A GEGF+ +SMGF NTAGP+GHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ
Sbjct: 384 TATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQ 443

Query: 424 FYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCR 483
           +YR+CVI GTVDFIFGD++ I QN  I +R+ +  Q+NTVTAQGR ++ + TG VIHNC 
Sbjct: 444 YYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCT 503

Query: 484 IVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGNFALETCLYL 543
           + P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   +FA++T  Y 
Sbjct: 504 VAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYA 563

Query: 544 EYGNRGPGANTNRRVRWKGVKVIGRNEALQYTAGAFLLGRTWLPRTGGIYYLGL 566
           EY N GP   T  RV+W G +V+ + EA+++T G FL G  W+   G    LGL
Sbjct: 564 EYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of MELO3C005195 vs. TrEMBL
Match: A0A0A0KJ73_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G125700 PE=4 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 1.8e-310
Identity = 529/567 (93.30%), Postives = 549/567 (96.83%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
            TL+HVA+NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDC DL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTD+DR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+TAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLASKQ GVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of MELO3C005195 vs. TrEMBL
Match: A0A0A0KLN2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G119700 PE=4 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 4.4e-275
Identity = 469/567 (82.72%), Postives = 517/567 (91.18%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           MVGKV++S +SLILVVG+ALAVVA +NK + S+     +SPKMKA+++ICS  DYQ EC+
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
            TL  V  N+S  DPK++ KAAI +TI+E+ KGYNL+D+L+VEA NNA+IKMSV+DC DL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYSTVG+ DLHT+ DRIADIKNWL+AV+SYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+ AVS+IL   GLQLKVQPSGRRLLGTTEVDSD FPTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLA+K+GGVR  PN VVAKDGSGQYKTI AALAAYPKAL+GRYVIYVKAG+Y+E I VT
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKN+FMYGDGPRKTIVTGRKSNRDG TTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFAL TC Y EYGNRGPGANTNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           Q+TAG FLLG+ WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of MELO3C005195 vs. TrEMBL
Match: A5ATJ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040170 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 6.5e-202
Identity = 363/564 (64.36%), Postives = 444/564 (78.72%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGS--NGGKEEVSPKMKAISAICSTTDYQEE 60
           M+GK +VS +SLILVVG+ + +V+ + + +GS  N G   +S  MKA++++C+T DY++ 
Sbjct: 1   MIGKAVVSGISLILVVGVIIGIVS-VTRPHGSDRNDGNTNMSSSMKAVASVCATADYKDA 60

Query: 61  CKNTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCN 120
           C  TL+ VA+N S+  PKDY +AA+  T+KEI    NLS+ L V+A N++  +M++ DC 
Sbjct: 61  CMQTLSPVAKNGSAT-PKDYIQAAVQVTMKEIKSSMNLSEKL-VQATNDSRTQMALGDCK 120

Query: 121 DLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQR 180
           DLLQFAIDELQ S+S+VGESDL T      +I NWLSAVVSYQQ+CLDG+   +P+ +  
Sbjct: 121 DLLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAVVSYQQTCLDGV--IEPRFQTA 180

Query: 181 MQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEV-DSDGFPTWLTG 240
           MQ GL  A +LTSNALAI++ +S IL  + + L ++P+ RRLLG  +V   DG+PTW + 
Sbjct: 181 MQKGLLNATQLTSNALAIVSDISQILTKFNVSLDLKPNSRRLLGEIDVLGHDGYPTWFSA 240

Query: 241 ADRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYI 300
            DRKLLAS   G R TPNA+VAKDGSG + TIAAALAAYPK L+GRYVIYVKAGIY EYI
Sbjct: 241 TDRKLLASHDNG-RLTPNAIVAKDGSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYI 300

Query: 301 TVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQ 360
           TVTKD  NV+MYGDGPRKTIVTG KS RDG TT  TA+F+AIG+GF+ +SMGF NTAGP 
Sbjct: 301 TVTKDQVNVYMYGDGPRKTIVTGTKSYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPD 360

Query: 361 GHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNS 420
           GHQAVALRVQSD SA FNCRMDG+QDTLY+Q HRQFYRNCVISGT+DFIFGDS+T+IQNS
Sbjct: 361 GHQAVALRVQSDMSAIFNCRMDGYQDTLYIQAHRQFYRNCVISGTIDFIFGDSTTVIQNS 420

Query: 421 LIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQY 480
           LIIVRRP DNQQNTVTA G+AE++E TGLVIHNCRIVPEQKLF +RFKIP+FLGRPWK Y
Sbjct: 421 LIIVRRPKDNQQNTVTAHGKAEKRETTGLVIHNCRIVPEQKLFPDRFKIPSFLGRPWKPY 480

Query: 481 ARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI-GR 540
           ++T+IME+TLGDFIQPAG+MPW+G+FAL T  Y EYGNRGPGANT  RV WKG ++I  R
Sbjct: 481 SKTIIMETTLGDFIQPAGWMPWAGDFALNTLFYAEYGNRGPGANTRSRVTWKGYRIIKTR 540

Query: 541 NEALQYTAGAFLLGRTWLPRTGGI 561
           NEALQYT  +    RT   R G +
Sbjct: 541 NEALQYTPSS---PRTRSARKGSV 555

BLAST of MELO3C005195 vs. TrEMBL
Match: A5ATJ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040170 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 3.3e-190
Identity = 342/538 (63.57%), Postives = 415/538 (77.14%), Query Frame = 1

Query: 25   TINKRNGSN--GGKEEVSPKMKAISAICSTTDYQEECKNTLNHVAQNTSSNDPKDYAKAA 84
            ++ + +GS+   G   +S  MKA++++C+T DY++ C  TL+ V +N SS  PKDY +AA
Sbjct: 554  SVTRPHGSDRTDGDTNMSSSMKAVASVCATADYKDACMQTLSPVPKNGSSATPKDYIQAA 613

Query: 85   ILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDLLQFAIDELQASYSTVGESDLHT 144
            +  TIK+I    NLS+ L  +A N++  +M++ DC DLLQFAIDELQ S+S+VGESDL T
Sbjct: 614  VQVTIKQIKSSMNLSEKLF-QATNDSRTQMALGDCKDLLQFAIDELQESFSSVGESDLQT 673

Query: 145  DADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAILTAVSN 204
                  +I NWLSA VSYQQ+CLDG+   +P+ +  MQ GL  A +LTSNALAI++ +S 
Sbjct: 674  LDQLSTEIMNWLSAAVSYQQTCLDGV--IEPRFQNAMQKGLLNATQLTSNALAIVSDLSQ 733

Query: 205  ILDNYGLQLKVQPSGRRLLGTTEV-DSDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKD 264
            IL  + + L ++P+ RRLLG  EV   DG+PTW +  DRKLLA +  G R TPNA+VAKD
Sbjct: 734  ILTKFNVPLDLKPNSRRLLGEIEVLGHDGYPTWFSATDRKLLALQDNG-RLTPNAIVAKD 793

Query: 265  GSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGR 324
            GSG + TIAAALAAYPK L+GRYVIYVKAGIY EYITVTKD  NV+MYGDGPRKTIVTG 
Sbjct: 794  GSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYITVTKDHVNVYMYGDGPRKTIVTGT 853

Query: 325  KSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGH 384
            K  RDG TT  TA+F+AIG+GF+ +SMGF NTAGP GHQAVALRVQSD SAFFNCRMDG+
Sbjct: 854  KCYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDGHQAVALRVQSDMSAFFNCRMDGY 913

Query: 385  QDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQK 444
            QDTLYVQ HRQFYRNCVISGT+DFIFGDS+T+IQNSLIIVRRP D QQNTVTAQG+ E++
Sbjct: 914  QDTLYVQAHRQFYRNCVISGTIDFIFGDSTTVIQNSLIIVRRPNDKQQNTVTAQGKTEKR 973

Query: 445  EITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSG 504
            E TGLVIH+CRIVPEQKLF +RFKIP+FLGRPWK Y++T+IME+TLGDFIQPAG+ PW+G
Sbjct: 974  ETTGLVIHDCRIVPEQKLFPDRFKIPSFLGRPWKPYSKTIIMETTLGDFIQPAGWTPWAG 1033

Query: 505  NFALETCLYLEYGNRGPGANTNRRVRWKGVKVI-GRNEALQYTAGAFLLGRTWLPRTG 559
             F   T LY EYGN GPGANT+ RV WKG ++I  RNEALQYT       R  L   G
Sbjct: 1034 KFVPNTLLYAEYGNLGPGANTHSRVTWKGYRIIKTRNEALQYTVSNVEAQRVGLEERG 1087


HSP 2 Score: 708.0 bits (1826), Expect = 9.4e-201
Identity = 359/570 (62.98%), Postives = 441/570 (77.37%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSN--GGKEEVSPKMKAISAICSTTDYQEE 60
           M+GK +VS +SL LVVG+ + +V+ + + +GS+   G   +S  MKA++++C+T DY++ 
Sbjct: 249 MIGKAVVSGISLFLVVGVIIGIVS-VTRPHGSDRTDGDTNMSSSMKAVASVCATADYKDA 308

Query: 61  CKNTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCN 120
           C  TL+ V +N SS  PKDY +AA+  TIK+I    NLS+ L  +A N++  +M++ DC 
Sbjct: 309 CMQTLSPVPKNGSSATPKDYIQAAVQVTIKQIKSSMNLSEKLF-QATNDSRTQMALGDCK 368

Query: 121 DLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQR 180
           DLLQFAIDELQ S+S+VGESDL T      +I NWLSA VSYQQ+CLDG+   +P+ +  
Sbjct: 369 DLLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAAVSYQQTCLDGV--IEPRFQAA 428

Query: 181 MQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEV-DSDGFPTWLTG 240
           MQ GL  A +LTSNALAI++ +S IL  + + L ++P+ RRLLG  EV   DG+PTW + 
Sbjct: 429 MQKGLLNATQLTSNALAIVSDLSQILTKFNVPLDLKPNSRRLLGEIEVLGHDGYPTWFSA 488

Query: 241 ADRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYI 300
            DRKLLA +  G R TPNA+VAKDGSG + TIAAALAAYPK L+GRYVIYVKAGIY EYI
Sbjct: 489 TDRKLLALQDNG-RLTPNAIVAKDGSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYI 548

Query: 301 TVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQ 360
           TVTKD  NV+MYGDGPRKTIVTG K  RDG TT  TA+F+AIG+GF+ +SMGF NTAGP 
Sbjct: 549 TVTKDHVNVYMYGDGPRKTIVTGTKCYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPD 608

Query: 361 GHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNS 420
           GHQAVALRVQSD SAFFNCRMDG+QDTLYVQ HRQFYRNCVISGT+DFIFGDS+T+IQNS
Sbjct: 609 GHQAVALRVQSDMSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTIDFIFGDSTTVIQNS 668

Query: 421 LIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQY 480
           LIIVRRP D QQNTVTAQG+ E++E TGLVIH+CRIVPEQKLF +RFKIP+FLGRPWK Y
Sbjct: 669 LIIVRRPNDKQQNTVTAQGKTEKRETTGLVIHDCRIVPEQKLFPDRFKIPSFLGRPWKPY 728

Query: 481 ARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI-GR 540
           ++T+IME+TLGDFIQPAG+ PW+G F   T LY EYGN GPGANT+ RV WKG ++I  R
Sbjct: 729 SKTIIMETTLGDFIQPAGWTPWAGKFVPNTLLYAEYGNLGPGANTHSRVTWKGYRIIKTR 788

Query: 541 NEALQYTAGAFLLGRTWLPRTGGIYYLGLK 567
           NEALQYT  +F+ G  WL +    Y  GLK
Sbjct: 789 NEALQYTVNSFIQGNLWLKQINIPYLPGLK 813

BLAST of MELO3C005195 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.2e-107
Identity = 187/237 (78.90%), Postives = 211/237 (89.03%), Query Frame = 1

Query: 308 MYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQ 367
           MYGDGPRKTIVTG KS RDG TT  TA+F+AIG+GF+ +SMGF NTAGP GHQAVALRVQ
Sbjct: 1   MYGDGPRKTIVTGTKSYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDGHQAVALRVQ 60

Query: 368 SDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDN 427
           SD SA FNCRMDG+QDTLY+Q HRQFYRNCVISGT+DFIFGDS+T+IQNSLIIVRRP DN
Sbjct: 61  SDMSAIFNCRMDGYQDTLYIQAHRQFYRNCVISGTIDFIFGDSTTVIQNSLIIVRRPKDN 120

Query: 428 QQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTL 487
           QQNTVTA G+AE++E TGLVIHNCRIVPEQKLF +RFKIP+FLGRPWK Y++T+IME+TL
Sbjct: 121 QQNTVTAHGKAEKRETTGLVIHNCRIVPEQKLFPDRFKIPSFLGRPWKPYSKTIIMETTL 180

Query: 488 GDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVI-GRNEALQYT 544
           GDFIQPAG+MPW+G+FAL T  Y EYGNRGPGANT  RV WKG ++I  RNEALQYT
Sbjct: 181 GDFIQPAGWMPWAGDFALNTLFYAEYGNRGPGANTRSRVTWKGYRIIKTRNEALQYT 237


HSP 2 Score: 706.8 bits (1823), Expect = 2.1e-200
Identity = 352/571 (61.65%), Postives = 447/571 (78.28%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKR-NGSNGGKEEVSPKMKA-ISAICSTTDYQEE 60
           +VGKV+VS +S++L+VG+ALAVV  +N   NG + G        K+ + ++CS TDY+  
Sbjct: 3   IVGKVVVSVVSVLLLVGVALAVVVVVNNHGNGKHEGSVAAPSTSKSMVESVCSNTDYKTA 62

Query: 61  CKNTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNAS--IKMSVED 120
           C+ TL+ ++ N ++ D KD  KAA+ AT+ +++K  NLS+SL+VEA NN S  +KM++ED
Sbjct: 63  CEQTLSPLSHNVTA-DYKDIIKAAVQATVDQVSKSLNLSESLLVEANNNNSTRLKMALED 122

Query: 121 CNDLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLK 180
           CNDLL  AI +LQAS+S VG+SD+HT  DR A++++WLS+V+SY ++C+DG+ E  P+ +
Sbjct: 123 CNDLLSLAIGQLQASFSMVGDSDMHTIKDRSAELRSWLSSVISYAETCIDGVEE--PKFQ 182

Query: 181 QRMQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQP-SGRRLLGTTEVDSDGFPTWL 240
            +++  L  A  LT NALAI++ +S IL  +GL+L  +  +GRRLL  T + +DG PTW 
Sbjct: 183 GQLKGTLQNATALTDNALAIVSTISEILSAFGLKLNPKALNGRRLLSDTVIGNDGLPTWF 242

Query: 241 TGADRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDE 300
           + +DRKL+A   G  R  P+AVVAKDGSGQ+KTIAAALAAYPK   GRY+IYVKAG+Y+E
Sbjct: 243 SASDRKLMA-LHGNGRIKPHAVVAKDGSGQFKTIAAALAAYPKNHVGRYIIYVKAGVYNE 302

Query: 301 YITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAG 360
           YITV K+M N+FMYGDGPRKTIVTGRKS   GF+T  TASF+ IG GF+CK MGF NTAG
Sbjct: 303 YITVEKNMVNIFMYGDGPRKTIVTGRKSYTGGFSTFRTASFSVIGAGFVCKGMGFQNTAG 362

Query: 361 PQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQ 420
           P+GHQAVALRVQS+ SAFFNCRMDG+QDTLYVQ  RQFYRNCVISGT+DFIFGD++ +IQ
Sbjct: 363 PEGHQAVALRVQSEMSAFFNCRMDGYQDTLYVQAGRQFYRNCVISGTIDFIFGDATVVIQ 422

Query: 421 NSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK 480
           NSL+IVRRPMDNQ N VTAQGR E+ + TG+VIHNCRIVPEQKLFA+RFKIPT+LGRPWK
Sbjct: 423 NSLLIVRRPMDNQLNAVTAQGRKERHQPTGIVIHNCRIVPEQKLFADRFKIPTYLGRPWK 482

Query: 481 QYARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIG 540
           +++RTVIMESTL DFIQPAG+ PW+GNFAL+T  Y EY NRGPGA TNRRV W+G  VIG
Sbjct: 483 EFSRTVIMESTLADFIQPAGWFPWAGNFALDTLYYAEYANRGPGARTNRRVHWRGYHVIG 542

Query: 541 RNEALQYTAGAFLLGRTWLPRTGGIYYLGLK 567
           R+EA Q+T G FL G+ WLP TG  +    K
Sbjct: 543 RHEAQQFTVGQFLEGQRWLPATGAPFLPSFK 569

BLAST of MELO3C005195 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 458.4 bits (1178), Expect = 6.5e-129
Identity = 228/565 (40.35%), Postives = 350/565 (61.95%), Query Frame = 1

Query: 4   KVIVSALSLILVVGIALAVVA--TINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECKN 63
           + IV  +S +L++ + +AV    ++NK +G + GK EV+  +KA+  +C+ TDY++ C++
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDLL 123
           TL    +NT+  DP +  K A   T+K+IT     S + I+E   ++  +M+++ C +L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELM 133

Query: 124 QFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQD 183
            +A+DEL  S+  +G+ + H   + + +++ WLSA +S++++CL+G         + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 GLDVAGKLTSNALAILTAVSNILDNYGLQLKVQP-SGRRLLGTTEVDSDGFPTWLTGADR 243
            L  A +LT N LAI++ +SN +     Q+++   + RRLL      ++GFP+W+    R
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVG----QMQIPGLNSRRLL------AEGFPSWVDQRGR 253

Query: 244 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 303
           KLL +        P+ VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y EY+ V 
Sbjct: 254 KLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVN 313

Query: 304 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 363
           K M ++   GDGP KTI++G K+ +DG TT  TA+ A +G  F+ K++GF NTAG   HQ
Sbjct: 314 KTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQ 373

Query: 364 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 423
           AVA+RVQSD S FFNCR DG+QDTLY  +HRQF+R+C ISGT+DF+FGD++ + QN  ++
Sbjct: 374 AVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLL 433

Query: 424 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 483
           VR+P+ NQ   +TA GR + +E TG V   C I  E    A +     +LGRPWK+Y+RT
Sbjct: 434 VRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRT 493

Query: 484 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 543
           +IM + + DF+QP G+ PW G+F L+T  Y E  N GPG+    RV W G+K +   + L
Sbjct: 494 IIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDIL 553

Query: 544 QYTAGAFLLGRTWLPRTGGIYYLGL 566
           ++T   ++ G  W+P  G  Y  GL
Sbjct: 554 KFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of MELO3C005195 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 457.2 bits (1175), Expect = 1.5e-128
Identity = 227/562 (40.39%), Postives = 345/562 (61.39%), Query Frame = 1

Query: 4   KVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECKNTL 63
           + ++ ++S +L++ + +AV   ++     N G EE++  +KAI  +C+ TDY+E C++TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  NHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDLLQF 123
              A++TS  DP +  K A  AT+K+I+     S ++I E   +   KM+++ C +L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQDGL 183
           AI EL  S+  +G+ + H   + +  ++ WLSA +S++Q+CLDG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADRKLL 243
             A +LT N LA++T +SN L    +    + + RRLL      S  FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRLL 254

Query: 244 ASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVTKDM 303
            +    V+P  + VVA+DGSGQYKTI  AL   PK     +V+++K GIY EY+ V + M
Sbjct: 255 NAPMSEVKP--DIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVA 363
            ++   GDGP KT+++G KS +DG TT  TA+ A +G+ F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C ISGT+DF+FGD++ + QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA GR + +E TG V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEALQYT 543
            + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G+K +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGAFLLGRTWLPRTGGIYYLGL 566
              ++ G  W+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of MELO3C005195 vs. TAIR10
Match: AT2G47030.1 (AT2G47030.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 254/601 (42.26%), Postives = 347/601 (57.74%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           M+GKV+VS  S++L+VG+A+ VVA INK   +N     +SP+MKA+  IC +T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDAN-----LSPQMKAVQGICQSTSDKASCV 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASI--KMSVEDCN 120
            TL  V     S DP    KA +LAT  E+TK  N +    V  G++ S   K  ++ C 
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQR 180
            +  +A+++L      +GE DL     +I  +K WL  V +YQ  CLD + E D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDVAGKLTSNALAIL-TAVSNI--LDNYGLQLK--------------------VQP 240
           + +G+  +  LT+NA+ I  T VS +  ++N    LK                      P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SGRRLLGTTEVDSDGFPTWLTGADRKLLA--------SKQGGVRPTPNAVVAKDGSGQYK 300
            G       ++D  G PTW++GADRKL+A         + GG R   N VVAKDGSGQ+K
Sbjct: 241 DGPARRLLEDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQFK 300

Query: 301 TIAAALAAYPKALRGRYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGRKS--NR 360
           T+  A+ A P+  RGR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S    
Sbjct: 301 TVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVALS 360

Query: 361 DGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGHQDTL 420
            G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QDTL
Sbjct: 361 RGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDTL 420

Query: 421 YVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQKEI-T 480
           YV   RQFYRNCV+SGTVDFIFG S+T+IQN+LI+VR+    Q NTVTA G      +  
Sbjct: 421 YVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMKI 480

Query: 481 GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGNFA 540
           G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G   
Sbjct: 481 GIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGESF 540

Query: 541 LETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEALQYTAGAFLLGRTWLPRTGGIYYLG 566
            ++C Y+EY NRGPGA  NRRV W  V      E   +TA  +L    W+        +G
Sbjct: 541 HKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTIG 588

BLAST of MELO3C005195 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 435.3 bits (1118), Expect = 5.9e-122
Identity = 233/558 (41.76%), Postives = 339/558 (60.75%), Query Frame = 1

Query: 6   IVSALSLILVVGIALAVVATINKRNGSNGGK---EEVSPKMKAISAICSTTDYQEECKNT 65
           +++AL +++VV + +        RN S+  K    ++     A+ A+C+ TDY+E C N+
Sbjct: 17  VITALLVLMVVAVGITT-----SRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNS 76

Query: 66  LNHVAQNTSSNDPKDYAKAAILATIKEITKGYN-LSDSLIVEAGNNASIKMSVEDCNDLL 125
           L  +  +  S  P D  K     TI+ I       S  L  +A N+   K ++E C  L+
Sbjct: 77  L--MKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLM 136

Query: 126 QFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQD 185
             A D+L+          +    D + D++ WLS  ++YQQ+C+D   E + +L Q MQ 
Sbjct: 137 NDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQK 196

Query: 186 GLDVAGKLTSNALAILTAVSNILDNY---GLQLKVQPSGRRLLGTTEVDSDGFPTWLTGA 245
               + +LTSN LA++T +SN+L  +   G+   +    R+LL       DG P+W+   
Sbjct: 197 IFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA----EDGIPSWVGPN 256

Query: 246 DRKLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYIT 305
            R+L+A+K GGV+   N VVA DGSGQYKTI  AL A PKA +  +VIY+K G+Y+E + 
Sbjct: 257 TRRLMATK-GGVKA--NVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVD 316

Query: 306 VTKDMKNVFMYGDGPRKTIVTGRKSNRDG-FTTQNTASFAAIGEGFLCKSMGFTNTAGPQ 365
           VTK M +V   GDGP KT +TG  +   G   T  TA+ A  G+ F  K++GF NTAGP+
Sbjct: 317 VTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPE 376

Query: 366 GHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNS 425
           GHQAVALRV +D + F+NC++DG+QDTLYV +HRQF+R+C +SGTVDFIFGD   ++QN 
Sbjct: 377 GHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNC 436

Query: 426 LIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQY 485
            I+VR+PM +Q   +TAQGR++++E TGLV+ NC I  E      +     +LGRPWK++
Sbjct: 437 NIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEF 496

Query: 486 ARTVIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRN 545
           +RT+IM +T+ D I PAG++PW+G+FAL T  Y EY N GPG+N  +RV+W G+K +   
Sbjct: 497 SRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPK 556

Query: 546 EALQYTAGAFLLGRTWLP 556
           +AL++T   FL G  W+P
Sbjct: 557 QALRFTPARFLRGNLWIP 560

BLAST of MELO3C005195 vs. TAIR10
Match: AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-120
Identity = 246/594 (41.41%), Postives = 352/594 (59.26%), Query Frame = 1

Query: 4   KVIVSALSLILV----VGIALAVVA-----------TINKRNGSNGGKEEVSPKM----- 63
           ++I+  +S+++V    +G A A V            T NK   S    E  SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAISAICSTTDYQEECKNTL-NHVAQNTSSNDPKDYAKAAILATIKEITKG 123
                    K I  +C++T Y+  C+NTL N   ++T   DP+   K+AI+A   ++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLSDSLIVEAGNNASIKMSVEDCNDLLQFAIDELQASYSTVGESDLHTDADRIADIKNW 183
           +    SL  E   N   K ++  C  L+  A +EL  S   + +S+++  A  + D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LSAVVSYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAILTAVSNILDNYGLQLKV 243
           LSAV+SYQ++C+DG  E   +LK  ++   + +  LTSN+LA++ ++   L +     KV
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KV 263

Query: 244 QPSGRRLLGTTEVDSDGFPTWLTGADRKLLASKQGGVRPT-PNAVVAKDGSGQYKTIAAA 303
           +        ++  ++D   +WL+  +R++L  K   V+   PNA VAKDGSG + TI AA
Sbjct: 264 KTRLLLEARSSAKETDHITSWLSNKERRML--KAVDVKALKPNATVAKDGSGNFTTINAA 323

Query: 304 LAAYPKALRGRYVIYVKAGIYDEYITVTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQN 363
           L A P   +GRY IY+K GIYDE + + K   NV M GDG +KTIVTG KS+     T  
Sbjct: 324 LKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFL 383

Query: 364 TASFAAIGEGFLCKSMGFTNTAGPQGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQ 423
           TA+F A GEGF+ +SMGF NTAGP+GHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ
Sbjct: 384 TATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQ 443

Query: 424 FYRNCVISGTVDFIFGDSSTIIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCR 483
           +YR+CVI GTVDFIFGD++ I QN  I +R+ +  Q+NTVTAQGR ++ + TG VIHNC 
Sbjct: 444 YYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCT 503

Query: 484 IVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGNFALETCLYL 543
           + P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   +FA++T  Y 
Sbjct: 504 VAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYA 563

Query: 544 EYGNRGPGANTNRRVRWKGVKVIGRNEALQYTAGAFLLGRTWLPRTGGIYYLGL 566
           EY N GP   T  RV+W G +V+ + EA+++T G FL G  W+   G    LGL
Sbjct: 564 EYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of MELO3C005195 vs. NCBI nr
Match: gi|659072607|ref|XP_008466403.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
           NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
           DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           QYTAGAFLLGRTWLPRTGGIYYLGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of MELO3C005195 vs. NCBI nr
Match: gi|449463555|ref|XP_004149499.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 1072.4 bits (2772), Expect = 2.7e-310
Identity = 529/567 (93.30%), Postives = 549/567 (96.83%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
            TL+HVA+NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDC DL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTD+DR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+TAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLASKQ GVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of MELO3C005195 vs. NCBI nr
Match: gi|778698533|ref|XP_004149498.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 954.9 bits (2467), Expect = 6.3e-275
Identity = 469/567 (82.72%), Postives = 517/567 (91.18%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           MVGKV++S +SLILVVG+ALAVVA +NK + S+     +SPKMKA+++ICS  DYQ EC+
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
            TL  V  N+S  DPK++ KAAI +TI+E+ KGYNL+D+L+VEA NNA+IKMSV+DC DL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYSTVG+ DLHT+ DRIADIKNWL+AV+SYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+ AVS+IL   GLQLKVQPSGRRLLGTTEVDSD FPTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLA+K+GGVR  PN VVAKDGSGQYKTI AALAAYPKAL+GRYVIYVKAG+Y+E I VT
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKN+FMYGDGPRKTIVTGRKSNRDG TTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGNFAL TC Y EYGNRGPGANTNRRVRWKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           Q+TAG FLLG+ WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of MELO3C005195 vs. NCBI nr
Match: gi|449467649|ref|XP_004151535.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 948.0 bits (2449), Expect = 7.7e-273
Identity = 458/567 (80.78%), Postives = 516/567 (91.01%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           MV KV++  +SL+LVVG+ALAVVA +N  N S+   E++SPKMKA+S ICST +YQEEC+
Sbjct: 1   MVNKVVLPGISLLLVVGVALAVVAIVNS-NKSSDATEDLSPKMKAVSTICSTANYQEECQ 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
           NTL + A N SS+DPK+Y KAAILATI E+ KGYNL+D  ++EA NN SIKM VEDC DL
Sbjct: 61  NTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAID+LQASYSTVGE DLHT+ADR+ADIKNWL++V+SYQQSCLDGL EFDPQL+Q+MQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
           DGL+ AGKLTSNALAI+ AVS+IL ++GLQLK QPSGRRLLGTTEVD+DG+PTWLTGADR
Sbjct: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLAS+ GG +  PNAVVAKDGSGQ+KTI+AALAAYPK LRGRYVIYVKAGIY EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR GFTTQ+TA+F AIGEGFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCVISGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIMEST+GDFIQP GYMPWSGNFALETCLYLEYGNRGPGA TNRRVRWKGVKVIGRNEA+
Sbjct: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           Q+TAG+FL G+TWLP TGG Y LGLK+
Sbjct: 541 QFTAGSFLQGKTWLPTTGGPYLLGLKS 566

BLAST of MELO3C005195 vs. NCBI nr
Match: gi|659072603|ref|XP_008466382.1| (PREDICTED: pectinesterase-like isoform X2 [Cucumis melo])

HSP 1 Score: 947.2 bits (2447), Expect = 1.3e-272
Identity = 466/567 (82.19%), Postives = 518/567 (91.36%), Query Frame = 1

Query: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60
           MV KV+++  SL LVVG+ LAV A +NK N S+    ++SPKMKA+S+ICS TDYQ+EC+
Sbjct: 1   MVAKVVMAVTSL-LVVGVVLAVFAMVNK-NHSSDETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120
            TL +V  N+S +DPK++ KAAI ATI+E+ KGYNL+D+L+VEA NNASIKM+V+DC DL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYSTVG+ DLHT+ DR+ADIKNWL+AV+SYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240
            GLDVA KLTSNALAI+ AVS+IL + GLQLKVQPSGRRLLGTTEVD+DGFPTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300
           KLLA+K+GGVR  PNAVVAKDGSGQYKTIAAALAAYPKAL+GRYVIYVKAGIY+EYI VT
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDG  TQNTA+FAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCV+SGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540
           VIME+TLGDFIQPAGYMPWSG+FAL TC Y EYGNRGPGANT RRVRWKGV+VIGRNEA+
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 568
           Q+TAG FLLGR WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME21_ARATH1.2e-12740.35Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=P... [more]
PME28_ARATH2.6e-12740.39Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=P... [more]
PME4_ARATH2.1e-12142.26Pectinesterase 4 OS=Arabidopsis thaliana GN=PME4 PE=2 SV=1[more]
PME58_ARATH1.1e-12041.76Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=P... [more]
PME45_ARATH2.6e-11941.41Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=P... [more]
Match NameE-valueIdentityDescription
A0A0A0KJ73_CUCSA1.8e-31093.30Pectinesterase OS=Cucumis sativus GN=Csa_5G125700 PE=4 SV=1[more]
A0A0A0KLN2_CUCSA4.4e-27582.72Pectinesterase OS=Cucumis sativus GN=Csa_5G119700 PE=4 SV=1[more]
A5ATJ7_VITVI6.5e-20264.36Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040170 PE=4 SV=1[more]
A5ATJ7_VITVI3.3e-19063.57Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040170 PE=4 SV=1[more]
F6HZG5_VITVI1.2e-10778.90Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT3G05610.16.5e-12940.35 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G27870.11.5e-12840.39 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT2G47030.11.2e-12242.26 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT5G49180.15.9e-12241.76 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G33230.11.5e-12041.41 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|659072607|ref|XP_008466403.1|0.0e+00100.00PREDICTED: pectinesterase-like [Cucumis melo][more]
gi|449463555|ref|XP_004149499.1|2.7e-31093.30PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|778698533|ref|XP_004149498.2|6.3e-27582.72PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|449467649|ref|XP_004151535.1|7.7e-27380.78PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|659072603|ref|XP_008466382.1|1.3e-27282.19PREDICTED: pectinesterase-like isoform X2 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0007034 vacuolar transport
biological_process GO:0010468 regulation of gene expression
biological_process GO:0051083 'de novo' cotranslational protein folding
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0006449 regulation of translational termination
biological_process GO:0045490 pectin catabolic process
biological_process GO:0043086 negative regulation of catalytic activity
cellular_component GO:0005618 cell wall
cellular_component GO:0005840 ribosome
cellular_component GO:0018444 translation release factor complex
cellular_component GO:0005777 peroxisome
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0003747 translation release factor activity
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0045330 aspartyl esterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C005195T1MELO3C005195T1mRNA


Analysis Name: InterPro Annotations of melon
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 255..550
score: 5.6E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 43..200
score: 1.4
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..196
score: 3.8
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..196
score: 6.5
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 39..198
score: 3.1
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 44..200
score: 2.09
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 252..562
score: 5.23E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 252..560
score: 6.5E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 2..567
score:
NoneNo IPR availablePANTHERPTHR31707:SF9PECTINESTERASE 4-RELATEDcoord: 2..567
score: