CmoCh04G022770 (gene) Cucurbita moschata (Rifu)

BLAST of CmoCh04G022770 vs. Swiss-Prot
Match: PME4_ARATH (Pectinesterase 4 OS=Arabidopsis thaliana GN=PME4 PE=2 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.6e-122
Identity = 259/603 (42.95%), Postives = 348/603 (57.71%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTT--IQMSVDDCK 120
            TL        S+DP + +KA ++AT +E+TK  N T    V    + +   +  +D CK
Sbjct: 61  KTLEPVK----SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D+     ++  +K WL  V ++Q  CLD +EE   DLR+ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE--DDLRKA 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-----------------------VQP 240
           +   + ++  LT+NA+ I   VV  + K+  ++                          P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RRLLGTTEVGSDGFPTWLVGEDRKLLA--------ARGGAGRVKPNAVVAKDGSGQ 300
            G  RRLL   ++   G PTW+ G DRKL+A         RGG  RV+ N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKS-- 360
           FKT+  A+ A P+   GR +IY+KAG+Y E +I+ K   NIFM+GDG R T+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQD 420
              G TT  SA+     +GF+ K MGF NTAGP GHQA A+RV  D++  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANG-RADNRQ 480
           TLYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G       
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 NTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYARTVIMESTLGDLIQPEGYMPWAGN 540
             G+V+ NCRIVP++KL  +R  + T+LGRPWK+++ TVIM + +GDLI+PEG+  W G 
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQGTGGPFF 566
               +C Y+EY NRGPGA  NRRV W  V      E   FTA  +L    W+Q    P  
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVT 588

BLAST of CmoCh04G022770 vs. Swiss-Prot
Match: PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 9.5e-122
Identity = 252/599 (42.07%), Postives = 355/599 (59.27%), Query Frame = 1

Query: 4   KVVVSGVSFILVVGVALAVVAVV----------------NKRNDTAAEMQTLSPKM---- 63
           K ++ GV  +LVV  A+   A                  NK  D+  + ++ SPK     
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVAGICSGTDYPKECQSTLST-TAQNSTSDDPKQYLKAAIVATIEEITK 123
                     K +  +C+ T Y   CQ+TL   T +++   DP+  LK+AIVA  +++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GLNLTDTLRVEAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKT 183
                 +L+ E  D+     ++  CK L+  A + L  S   + D +++  A  V D+ +
Sbjct: 143 VFKRVLSLKTENKDDKD---AIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVISFQQSCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILE-----KL 243
           WLSAV+S+Q++C+DG EE    L+ +++   +S+  LTSN+LA++  + G L      K 
Sbjct: 203 WLSAVMSYQETCVDGFEEGK--LKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 GLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFK 303
            L L+ + S +         +D   +WL  ++R++L A      +KPNA VAKDGSG F 
Sbjct: 263 RLLLEARSSAKE--------TDHITSWLSNKERRMLKAVD-VKALKPNATVAKDGSGNFT 322

Query: 304 TIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDG 363
           TI AAL A P    GRY IY+K G+YDE +I+ K   N+ M GDG + TIVTG KS+   
Sbjct: 323 TINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKK 382

Query: 364 VTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYV 423
           + T  +A+F A G+GF+ +SMGF NTAGPEGHQAVA+RVQSD+S F NCR +G+QDTLY 
Sbjct: 383 IRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYA 442

Query: 424 QANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANGRADNRQNTGLV 483
             +RQ+YR+CVI GTVDFIFGD+  I QN  I +R+ +  QKNTVTA GR D  Q TG V
Sbjct: 443 YTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFV 502

Query: 484 IHNCRIVPEQKLFADRFKIPTFLGRPWKQYARTVIMESTLGDLIQPEGYMPW-AGNFALD 543
           IHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D+I P G++ W   +FA+D
Sbjct: 503 IHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAID 562

Query: 544 TCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQGTGGPFFVGL 566
           T  Y EY N GP   T  RVKW G RV+ + EAM+FT GPFLQG  W+Q  G P  +GL
Sbjct: 563 TLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of CmoCh04G022770 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 438.3 bits (1126), Expect = 1.2e-121
Identity = 227/567 (40.04%), Postives = 350/567 (61.73%), Query Frame = 1

Query: 4   KVVVSGVSFILVVGVALAVVAVV--NKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQS 63
           + +V  +S +L++ + +AV   V  NK +  +     ++  +KAV  +C+ TDY K C+ 
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDLL 123
           TL    +N+T  DP + +K A   T+++IT     + T+     D+ T +M++D CK+L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRT-RMALDQCKELM 133

Query: 124 QSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQG 183
             A+D L  S   +G  + H   + + +++ WLSA IS +++CL+G + T  +  + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 ELDSAGKLTSNALAILDEV---VGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGE 243
            L +A +LT N LAI+ E+   VG ++  GL      + RRLL      ++GFP+W+   
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQMQIPGL------NSRRLL------AEGFPSWVDQR 253

Query: 244 DRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYII 303
            RKLL A      VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EY+ 
Sbjct: 254 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 313

Query: 304 VTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEG 363
           V K M ++   GDGP  TI++G K+ +DG+TT  +A+ + +G  F+ K++GF NTAG   
Sbjct: 314 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 373

Query: 364 HQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSL 423
           HQAVA+RVQSD+S FFNCR DG+QDTLY  ++RQF+R+C ISGT+DF+FGD+  + QN  
Sbjct: 374 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 433

Query: 424 IIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYA 483
           ++VR+P+ NQ   +TA+GR D R++TG V   C I  E    A +     +LGRPWK+Y+
Sbjct: 434 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 493

Query: 484 RTVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNE 543
           RT+IM + + D +QP+G+ PW G+F L T  Y E  N GPG+    RV W G++ +   +
Sbjct: 494 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 553

Query: 544 AMQFTAGPFLQGNTWLQGTGGPFFVGL 566
            ++FT   ++QG+ W+ G G P+  GL
Sbjct: 554 ILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh04G022770 vs. Swiss-Prot
Match: PME13_ARATH (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=PME13 PE=2 SV=2)

HSP 1 Score: 432.2 bits (1110), Expect = 8.9e-120
Identity = 234/524 (44.66%), Postives = 334/524 (63.74%), Query Frame = 1

Query: 44  KAVAGICSGTDYPKECQSTLST-TAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRV 103
           K +  +CS T Y + C+ TL   T +    D+P  +LK+AI A  E++   L    +L+ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 104 EAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQ 163
           E  D+     +++ CK L++ A +   AS + +   ++++    V D+++WLSAV+S+Q+
Sbjct: 167 ENQDDKD---AIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLG 223
           +CLDG EE +  L+ +++  ++S+  LTSN+LA++       E L   +KV    R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPVMKVVE--RHLL- 286

Query: 224 TTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTG 283
                 D  P+W+  +DR++L A      +KPNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVD-VKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKG 343
           RY+IYVK G+YDEY+ V K   N+ M GDG + TIVTG KS+   + T  +A+F A G+G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSD+S F NCR +G+QDTLY   +RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 404 VDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFAD 463
           +DFIFGD+  I QN  I +R+ +  QKNTVTA GR D  Q TG V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 464 RFKIPTFLGRPWKQYARTVIMESTLGDLIQPEGYMPW-AGNFALDTCLYLEYGNRGPGAM 523
           + +  ++LGRPWK Y+RT+IMES + ++I P G++ W   +FA+DT  Y EY N+G    
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 524 TNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQGTGGPFFVGL 566
           T  RVKW G +VI + EA+ +T GPFLQG+ W+  +G P  +GL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of CmoCh04G022770 vs. Swiss-Prot
Match: PME5_ARATH (Pectinesterase 5 OS=Arabidopsis thaliana GN=PME5 PE=1 SV=2)

HSP 1 Score: 430.6 bits (1106), Expect = 2.6e-119
Identity = 254/610 (41.64%), Postives = 350/610 (57.38%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA-----NLSPQMKAVRGICEATSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLR--VEAADNTTIQMSVDDCK 120
            TL        SDDP + +KA ++AT + IT+  N T      + +  +   +  +D CK
Sbjct: 61  KTLEPVK----SDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D++    ++  +K WL+ V ++Q  CLD +EE D  LR+ 
Sbjct: 121 KVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LRKT 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-------------------------- 240
           +   + S+  LTSNA+ I   VV  + KL L+++                          
Sbjct: 181 IGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPP 240

Query: 241 -------VQPSG--RRLLGTTEVGSDGFPTWLVGEDRKLLAARG-----GAGRVKPNAVV 300
                    P G  RRLL   ++   G PTW+ G DRKL+   G     G  R++   VV
Sbjct: 241 VADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVV 300

Query: 301 AKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIV 360
           AKDGSGQFKT+  A+ A P+   GR +I++KAG+Y E +I+ K   NIFM+GDG R T++
Sbjct: 301 AKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVI 360

Query: 361 TGRKSNR--DGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNC 420
           +  +S +   G TT  S +     +GF+ K +GF NTAGP GHQAVA+RV  D++  FNC
Sbjct: 361 SYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNC 420

Query: 421 RMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANG 480
           R DG+QDTLYV   RQFYRN V+SGTVDFIFG S T+IQNSLI+VR+    Q NTVTA+G
Sbjct: 421 RFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTADG 480

Query: 481 RADN-RQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYARTVIMESTLGDLIQPEG 540
                    G+V+ NCRIVP++KL A+R  + ++LGRPWK+++ TVI+ S +GD+I+PEG
Sbjct: 481 NEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRPEG 540

Query: 541 YMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQ 566
           +  W G     +C Y+EY NRGPGA+TNRRV W  +      E   FT   +L    W+Q
Sbjct: 541 WKIWDGESFHKSCRYVEYNNRGPGAITNRRVNWVKI-ARSAAEVNDFTVANWLGPINWIQ 595

BLAST of CmoCh04G022770 vs. TrEMBL
Match: A0A0A0KLN2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G119700 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 7.3e-262
Identity = 452/567 (79.72%), Postives = 504/567 (88.89%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKVV+SG+S ILVVGVALAVVA+VNK +D++ E   LSPKMKAVA ICS  DY  ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNK-SDSSDETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL +   NS+ +DPK+++KAAI +TIEE+ KG NLTD L VEAA+N TI+MSVDDCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAID LHAS STVGDPD+HT+ DR+ D+K WL+AVIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             LD AGKLTSNALAI+  V  IL++LGLQLKVQPSGRRLLGTTEV SD FPTWL G DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLAA+ G  RVKPN VVAKDGSGQ+KTI AALAAYPK L GRYVIYVKAGVY+E IIVT
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+NIFMYGDGPR TIVTGRKSNRDG+TTQ++ASF+AIG+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ +RQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTANGRAD ++ +GLVIHNCRIVPEQKLFA+RFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIMESTLGD IQP GYMPW+GNFAL TC Y EYGNRGPGA TNRRV+WKGVRVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           QFTAGPFL G  WL GTGGP+ +GLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CmoCh04G022770 vs. TrEMBL
Match: A0A0A0KJ73_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G125700 PE=4 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 7.8e-248
Identity = 425/567 (74.96%), Postives = 493/567 (86.95%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKV+VSG+S ILVVG+ALA+ A +NK N +    + +SPKMKAV+ ICS TDY  EC+
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL   A+N++S+DPK Y +AAI+ATI EITKG NL+D+L VEA+ N +I+MSV+DCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQ AID L AS S VG+ D+HTD+DRV D+K WLSAVIS+QQSCLDGL E DP L+Q+MQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             LD AGKLTSNALAI+  V  IL+   LQLKVQPSGRRLLGTT V  DGFPTWL G DR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLA++    R  PNAVVAKDGSG++KTIAAALAAYPKVL GRYVIYVKAG+YDEYI +T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+N+FMYGDGPR TIVTGRKSNRDG TTQ++ASF+AIG+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGHQDTLYVQ +RQFYRNCV+SGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTA GRA+ ++ TGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIMESTLGD IQP GYMPW+G+FAL+TCLYLEYGNRGPGA TNRRV+WKG +VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           Q+TAG FL G +WL  TGG +++GLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of CmoCh04G022770 vs. TrEMBL
Match: W9QN86_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_013873 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 4.3e-198
Identity = 359/571 (62.87%), Postives = 431/571 (75.48%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKA--VAGICSGTDYPKE 60
           ++GKVVVS VS +L+VGVALAVV VVN   +   E    +P      V  +CS TDY   
Sbjct: 3   IVGKVVVSVVSVLLLVGVALAVVVVVNNHGNGKHEGSVAAPSTSKSMVESVCSNTDYKTA 62

Query: 61  CQSTLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADN--TTIQMSVDD 120
           C+ TLS  + N T+ D K  +KAA+ AT+++++K LNL+++L VEA +N  T ++M+++D
Sbjct: 63  CEQTLSPLSHNVTA-DYKDIIKAAVQATVDQVSKSLNLSESLLVEANNNNSTRLKMALED 122

Query: 121 CKDLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLR 180
           C DLL  AI  L AS S VGD DMHT  DR  ++++WLS+VIS+ ++C+DG+EE  P  +
Sbjct: 123 CNDLLSLAIGQLQASFSMVGDSDMHTIKDRSAELRSWLSSVISYAETCIDGVEE--PKFQ 182

Query: 181 QKMQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQP-SGRRLLGTTEVGSDGFPTWL 240
            +++G L +A  LT NALAI+  +  IL   GL+L  +  +GRRLL  T +G+DG PTW 
Sbjct: 183 GQLKGTLQNATALTDNALAIVSTISEILSAFGLKLNPKALNGRRLLSDTVIGNDGLPTWF 242

Query: 241 VGEDRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDE 300
              DRKL+A  G  GR+KP+AVVAKDGSGQFKTIAAALAAYPK   GRY+IYVKAGVY+E
Sbjct: 243 SASDRKLMALHGN-GRIKPHAVVAKDGSGQFKTIAAALAAYPKNHVGRYIIYVKAGVYNE 302

Query: 301 YIIVTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAG 360
           YI V KNM NIFMYGDGPR TIVTGRKS   G +T  +ASFS IG GF+CK MGF NTAG
Sbjct: 303 YITVEKNMVNIFMYGDGPRKTIVTGRKSYTGGFSTFRTASFSVIGAGFVCKGMGFQNTAG 362

Query: 361 PEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQ 420
           PEGHQAVALRVQS+ SAFFNCRMDG+QDTLYVQA RQFYRNCVISGT+DFIFGD+  +IQ
Sbjct: 363 PEGHQAVALRVQSEMSAFFNCRMDGYQDTLYVQAGRQFYRNCVISGTIDFIFGDATVVIQ 422

Query: 421 NSLIIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWK 480
           NSL+IVRRPMDNQ N VTA GR +  Q TG+VIHNCRIVPEQKLFADRFKIPT+LGRPWK
Sbjct: 423 NSLLIVRRPMDNQLNAVTAQGRKERHQPTGIVIHNCRIVPEQKLFADRFKIPTYLGRPWK 482

Query: 481 QYARTVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIG 540
           +++RTVIMESTL D IQP G+ PWAGNFALDT  Y EY NRGPGA TNRRV W+G  VIG
Sbjct: 483 EFSRTVIMESTLADFIQPAGWFPWAGNFALDTLYYAEYANRGPGARTNRRVHWRGYHVIG 542

Query: 541 RNEAMQFTAGPFLQGNTWLQGTGGPFFVGLK 567
           R+EA QFT G FL+G  WL  TG PF    K
Sbjct: 543 RHEAQQFTVGQFLEGQRWLPATGAPFLPSFK 569

BLAST of CmoCh04G022770 vs. TrEMBL
Match: F6HE41_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_05s0020g00800 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 8.8e-191
Identity = 346/569 (60.81%), Postives = 431/569 (75.75%), Query Frame = 1

Query: 3   GKVVVSGVSFILVVGVALAVVAVVNKRNDTAA----EMQTLSPKMKAVAGICSGTDYPKE 62
           GKV V G+S ILVVGVA+ ++A ++ R    +    + + LS   KAVA ICS TDY ++
Sbjct: 4   GKVAVGGISIILVVGVAIGLIAGLHGRGGHKSTDGDDEKVLSTTTKAVAAICSPTDYKQQ 63

Query: 63  CQSTLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCK 122
           C S+  T A N ++  PK +L AAI  T++E+ + +  ++++ +    +   +M+ +DC+
Sbjct: 64  CISSFQTLANNHSAT-PKDFLMAAIDITMKEVKEAIGKSESIGLAGNSSGRQKMATEDCQ 123

Query: 123 DLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 182
           DLLQ AI  L AS S VGD  MHT  DR  D+K WLSAVIS+QQSC+DG +ET P+++  
Sbjct: 124 DLLQLAIGELQASFSMVGDAAMHTINDRAQDLKNWLSAVISYQQSCMDGFDET-PEVKSA 183

Query: 183 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGE 242
           +Q  L +A +LTSNALAI+ E+  IL    + L    S RRL   TE  SD +P+W    
Sbjct: 184 IQNGLLNATQLTSNALAIVSEISAILTSFNIPLNFTSSFRRLQEATEE-SDQYPSWFSAA 243

Query: 243 DRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYII 302
           DRKLL  R   G+V PNAVVA DGSGQ+K+I AALAAYPK L GRYVIYVKAG+YDEYI 
Sbjct: 244 DRKLLG-RVDNGKVTPNAVVALDGSGQYKSIGAALAAYPKNLNGRYVIYVKAGIYDEYIT 303

Query: 303 VTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEG 362
           + KN+ NIFMYGDGPR T+VTG+KS  DG+TT  +++FS IG GF+CKSMGF NTAGPEG
Sbjct: 304 IEKNLVNIFMYGDGPRKTMVTGKKSFLDGITTYKTSTFSVIGNGFICKSMGFRNTAGPEG 363

Query: 363 HQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSL 422
           HQAVALRVQSD SAFFNCRMDG+QDTLYVQA+RQFYRNCVISGTVDFIFGDS T+IQNSL
Sbjct: 364 HQAVALRVQSDYSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTVDFIFGDSTTLIQNSL 423

Query: 423 IIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYA 482
           IIVR+P+DNQ+NT+TA+GRA+ R+ TGLVI NCRIVPE+KLF  RFK P++LGRPWK Y+
Sbjct: 424 IIVRKPLDNQQNTITAHGRAEKRETTGLVIQNCRIVPEEKLFPTRFKTPSYLGRPWKAYS 483

Query: 483 RTVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVI-GRN 542
           RTV+MEST+GD I P G++PW GNFALDT  Y EY N+GPGA T +RVKWKG +VI  RN
Sbjct: 484 RTVVMESTMGDFIHPAGWLPWMGNFALDTLYYAEYANKGPGANTAQRVKWKGYKVITNRN 543

Query: 543 EAMQFTAGPFLQGNTWLQGTGGPFFVGLK 567
           EA+QFTAGPF+QGN WL+ TG P+F+G +
Sbjct: 544 EALQFTAGPFIQGNEWLRLTGAPYFLGFR 568

BLAST of CmoCh04G022770 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 7.0e-188
Identity = 343/569 (60.28%), Postives = 423/569 (74.34%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQT-LSPKMKAVAGICSGTDYPKEC 60
           MIGK VVSG+S  LVVGV + +V+V         +  T +S  MKAVA +C+  DY   C
Sbjct: 249 MIGKAVVSGISLFLVVGVIIGIVSVTRPHGSDRTDGDTNMSSSMKAVASVCATADYKDAC 308

Query: 61  QSTLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKD 120
             TLS   +N +S  PK Y++AA+  TI++I   +NL++ L  +A +++  QM++ DCKD
Sbjct: 309 MQTLSPVPKNGSSATPKDYIQAAVQVTIKQIKSSMNLSEKL-FQATNDSRTQMALGDCKD 368

Query: 121 LLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKM 180
           LLQ AID L  S S+VG+ D+ T      ++  WLSA +S+QQ+CLDG+ E  P  +  M
Sbjct: 369 LLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAAVSYQQTCLDGVIE--PRFQAAM 428

Query: 181 QGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEV-GSDGFPTWLVGE 240
           Q  L +A +LTSNALAI+ ++  IL K  + L ++P+ RRLLG  EV G DG+PTW    
Sbjct: 429 QKGLLNATQLTSNALAIVSDLSQILTKFNVPLDLKPNSRRLLGEIEVLGHDGYPTWFSAT 488

Query: 241 DRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYII 300
           DRKLLA +   GR+ PNA+VAKDGSG F TIAAALAAYPK L GRYVIYVKAG+Y EYI 
Sbjct: 489 DRKLLALQDN-GRLTPNAIVAKDGSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYIT 548

Query: 301 VTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEG 360
           VTK+  N++MYGDGPR TIVTG K  RDG+TT  +A+FSAIGKGF+ +SMGF NTAGP+G
Sbjct: 549 VTKDHVNVYMYGDGPRKTIVTGTKCYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDG 608

Query: 361 HQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSL 420
           HQAVALRVQSD SAFFNCRMDG+QDTLYVQA+RQFYRNCVISGT+DFIFGDS T+IQNSL
Sbjct: 609 HQAVALRVQSDMSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTIDFIFGDSTTVIQNSL 668

Query: 421 IIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYA 480
           IIVRRP D Q+NTVTA G+ + R+ TGLVIH+CRIVPEQKLF DRFKIP+FLGRPWK Y+
Sbjct: 669 IIVRRPNDKQQNTVTAQGKTEKRETTGLVIHDCRIVPEQKLFPDRFKIPSFLGRPWKPYS 728

Query: 481 RTVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVI-GRN 540
           +T+IME+TLGD IQP G+ PWAG F  +T LY EYGN GPGA T+ RV WKG R+I  RN
Sbjct: 729 KTIIMETTLGDFIQPAGWTPWAGKFVPNTLLYAEYGNLGPGANTHSRVTWKGYRIIKTRN 788

Query: 541 EAMQFTAGPFLQGNTWLQGTGGPFFVGLK 567
           EA+Q+T   F+QGN WL+    P+  GLK
Sbjct: 789 EALQYTVNSFIQGNLWLKQINIPYLPGLK 813

BLAST of CmoCh04G022770 vs. TAIR10
Match: AT2G47030.1 (AT2G47030.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 439.5 bits (1129), Expect = 3.1e-123
Identity = 259/603 (42.95%), Postives = 348/603 (57.71%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGDA-----NLSPQMKAVQGICQSTSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTT--IQMSVDDCK 120
            TL        S+DP + +KA ++AT +E+TK  N T    V    + +   +  +D CK
Sbjct: 61  KTLEPVK----SEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D+     ++  +K WL  V ++Q  CLD +EE   DLR+ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEE--DDLRKA 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-----------------------VQP 240
           +   + ++  LT+NA+ I   VV  + K+  ++                          P
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 SG--RRLLGTTEVGSDGFPTWLVGEDRKLLA--------ARGGAGRVKPNAVVAKDGSGQ 300
            G  RRLL   ++   G PTW+ G DRKL+A         RGG  RV+ N VVAKDGSGQ
Sbjct: 241 DGPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 FKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKS-- 360
           FKT+  A+ A P+   GR +IY+KAG+Y E +I+ K   NIFM+GDG R T+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQD 420
              G TT  SA+     +GF+ K MGF NTAGP GHQA A+RV  D++  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANG-RADNRQ 480
           TLYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G       
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 NTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYARTVIMESTLGDLIQPEGYMPWAGN 540
             G+V+ NCRIVP++KL  +R  + T+LGRPWK+++ TVIM + +GDLI+PEG+  W G 
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQGTGGPFF 566
               +C Y+EY NRGPGA  NRRV W  V      E   FTA  +L    W+Q    P  
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVT 588

BLAST of CmoCh04G022770 vs. TAIR10
Match: AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 438.7 bits (1127), Expect = 5.4e-123
Identity = 252/599 (42.07%), Postives = 355/599 (59.27%), Query Frame = 1

Query: 4   KVVVSGVSFILVVGVALAVVAVV----------------NKRNDTAAEMQTLSPKM---- 63
           K ++ GV  +LVV  A+   A                  NK  D+  + ++ SPK     
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVAGICSGTDYPKECQSTLST-TAQNSTSDDPKQYLKAAIVATIEEITK 123
                     K +  +C+ T Y   CQ+TL   T +++   DP+  LK+AIVA  +++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GLNLTDTLRVEAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKT 183
                 +L+ E  D+     ++  CK L+  A + L  S   + D +++  A  V D+ +
Sbjct: 143 VFKRVLSLKTENKDDKD---AIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVISFQQSCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILE-----KL 243
           WLSAV+S+Q++C+DG EE    L+ +++   +S+  LTSN+LA++  + G L      K 
Sbjct: 203 WLSAVMSYQETCVDGFEEGK--LKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 GLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFK 303
            L L+ + S +         +D   +WL  ++R++L A      +KPNA VAKDGSG F 
Sbjct: 263 RLLLEARSSAKE--------TDHITSWLSNKERRMLKAVD-VKALKPNATVAKDGSGNFT 322

Query: 304 TIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDG 363
           TI AAL A P    GRY IY+K G+YDE +I+ K   N+ M GDG + TIVTG KS+   
Sbjct: 323 TINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKK 382

Query: 364 VTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYV 423
           + T  +A+F A G+GF+ +SMGF NTAGPEGHQAVA+RVQSD+S F NCR +G+QDTLY 
Sbjct: 383 IRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYA 442

Query: 424 QANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANGRADNRQNTGLV 483
             +RQ+YR+CVI GTVDFIFGD+  I QN  I +R+ +  QKNTVTA GR D  Q TG V
Sbjct: 443 YTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFV 502

Query: 484 IHNCRIVPEQKLFADRFKIPTFLGRPWKQYARTVIMESTLGDLIQPEGYMPW-AGNFALD 543
           IHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D+I P G++ W   +FA+D
Sbjct: 503 IHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAID 562

Query: 544 TCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQGTGGPFFVGL 566
           T  Y EY N GP   T  RVKW G RV+ + EAM+FT GPFLQG  W+Q  G P  +GL
Sbjct: 563 TLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of CmoCh04G022770 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 438.3 bits (1126), Expect = 7.0e-123
Identity = 227/567 (40.04%), Postives = 350/567 (61.73%), Query Frame = 1

Query: 4   KVVVSGVSFILVVGVALAVVAVV--NKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQS 63
           + +V  +S +L++ + +AV   V  NK +  +     ++  +KAV  +C+ TDY K C+ 
Sbjct: 14  RYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCED 73

Query: 64  TLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDLL 123
           TL    +N+T  DP + +K A   T+++IT     + T+     D+ T +M++D CK+L+
Sbjct: 74  TLIKNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRT-RMALDQCKELM 133

Query: 124 QSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQG 183
             A+D L  S   +G  + H   + + +++ WLSA IS +++CL+G + T  +  + M+ 
Sbjct: 134 DYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKK 193

Query: 184 ELDSAGKLTSNALAILDEV---VGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGE 243
            L +A +LT N LAI+ E+   VG ++  GL      + RRLL      ++GFP+W+   
Sbjct: 194 ALKTAIELTHNGLAIISEMSNFVGQMQIPGL------NSRRLL------AEGFPSWVDQR 253

Query: 244 DRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYII 303
            RKLL A      VKP+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EY+ 
Sbjct: 254 GRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQ 313

Query: 304 VTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEG 363
           V K M ++   GDGP  TI++G K+ +DG+TT  +A+ + +G  F+ K++GF NTAG   
Sbjct: 314 VNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIK 373

Query: 364 HQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSL 423
           HQAVA+RVQSD+S FFNCR DG+QDTLY  ++RQF+R+C ISGT+DF+FGD+  + QN  
Sbjct: 374 HQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCT 433

Query: 424 IIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYA 483
           ++VR+P+ NQ   +TA+GR D R++TG V   C I  E    A +     +LGRPWK+Y+
Sbjct: 434 LLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYS 493

Query: 484 RTVIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNE 543
           RT+IM + + D +QP+G+ PW G+F L T  Y E  N GPG+    RV W G++ +   +
Sbjct: 494 RTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEED 553

Query: 544 AMQFTAGPFLQGNTWLQGTGGPFFVGL 566
            ++FT   ++QG+ W+ G G P+  GL
Sbjct: 554 ILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmoCh04G022770 vs. TAIR10
Match: AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 432.2 bits (1110), Expect = 5.0e-121
Identity = 234/524 (44.66%), Postives = 334/524 (63.74%), Query Frame = 1

Query: 44  KAVAGICSGTDYPKECQSTLST-TAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRV 103
           K +  +CS T Y + C+ TL   T +    D+P  +LK+AI A  E++   L    +L+ 
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 104 EAADNTTIQMSVDDCKDLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQ 163
           E  D+     +++ CK L++ A +   AS + +   ++++    V D+++WLSAV+S+Q+
Sbjct: 167 ENQDDKD---AIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLEETDPDLRQKMQGELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLG 223
           +CLDG EE +  L+ +++  ++S+  LTSN+LA++       E L   +KV    R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPVMKVVE--RHLL- 286

Query: 224 TTEVGSDGFPTWLVGEDRKLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTG 283
                 D  P+W+  +DR++L A      +KPNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVD-VKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKG 343
           RY+IYVK G+YDEY+ V K   N+ M GDG + TIVTG KS+   + T  +A+F A G+G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSD+S F NCR +G+QDTLY   +RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 404 VDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFAD 463
           +DFIFGD+  I QN  I +R+ +  QKNTVTA GR D  Q TG V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 464 RFKIPTFLGRPWKQYARTVIMESTLGDLIQPEGYMPW-AGNFALDTCLYLEYGNRGPGAM 523
           + +  ++LGRPWK Y+RT+IMES + ++I P G++ W   +FA+DT  Y EY N+G    
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 524 TNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQGTGGPFFVGL 566
           T  RVKW G +VI + EA+ +T GPFLQG+ W+  +G P  +GL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of CmoCh04G022770 vs. TAIR10
Match: AT2G47040.1 (AT2G47040.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-120
Identity = 254/610 (41.64%), Postives = 350/610 (57.38%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           MIGKVVVS  S +L+VGVA+ VVA +NK  D       LSP+MKAV GIC  T     C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAYINKNGDA-----NLSPQMKAVRGICEATSDKASCV 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLR--VEAADNTTIQMSVDDCK 120
            TL        SDDP + +KA ++AT + IT+  N T      + +  +   +  +D CK
Sbjct: 61  KTLEPVK----SDDPNKLIKAFMLATRDAITQSSNFTGKTEGNLGSGISPNNKAVLDYCK 120

Query: 121 DLLQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQK 180
            +   A++ L      +G+ D++    ++  +K WL+ V ++Q  CLD +EE D  LR+ 
Sbjct: 121 KVFMYALEDLSTIVEEMGE-DLNQIGSKIDQLKQWLTGVYNYQTDCLDDIEEDD--LRKT 180

Query: 181 MQGELDSAGKLTSNALAILDEVVGILEKLGLQLK-------------------------- 240
           +   + S+  LTSNA+ I   VV  + KL L+++                          
Sbjct: 181 IGEGIASSKILTSNAIDIFHTVVSAMAKLNLKVEDFKNMTGGIFAPSDKGAAPVNKGTPP 240

Query: 241 -------VQPSG--RRLLGTTEVGSDGFPTWLVGEDRKLLAARG-----GAGRVKPNAVV 300
                    P G  RRLL   ++   G PTW+ G DRKL+   G     G  R++   VV
Sbjct: 241 VADDSPVADPDGPARRLL--EDIDETGIPTWVSGADRKLMTKAGRGSNDGGARIRATFVV 300

Query: 301 AKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVTKNMENIFMYGDGPRSTIV 360
           AKDGSGQFKT+  A+ A P+   GR +I++KAG+Y E +I+ K   NIFM+GDG R T++
Sbjct: 301 AKDGSGQFKTVQQAVNACPEKNPGRCIIHIKAGIYREQVIIPKKKNNIFMFGDGARKTVI 360

Query: 361 TGRKSNR--DGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQAVALRVQSDKSAFFNC 420
           +  +S +   G TT  S +     +GF+ K +GF NTAGP GHQAVA+RV  D++  FNC
Sbjct: 361 SYNRSVKLSPGTTTSLSGTVQVESEGFMAKWIGFKNTAGPMGHQAVAIRVNGDRAVIFNC 420

Query: 421 RMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQKNTVTANG 480
           R DG+QDTLYV   RQFYRN V+SGTVDFIFG S T+IQNSLI+VR+    Q NTVTA+G
Sbjct: 421 RFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLIVVRKGNKGQFNTVTADG 480

Query: 481 RADN-RQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYARTVIMESTLGDLIQPEG 540
                    G+V+ NCRIVP++KL A+R  + ++LGRPWK+++ TVI+ S +GD+I+PEG
Sbjct: 481 NEKGLAMKIGIVLQNCRIVPDKKLAAERLIVESYLGRPWKKFSTTVIINSEIGDVIRPEG 540

Query: 541 YMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAMQFTAGPFLQGNTWLQ 566
           +  W G     +C Y+EY NRGPGA+TNRRV W  +      E   FT   +L    W+Q
Sbjct: 541 WKIWDGESFHKSCRYVEYNNRGPGAITNRRVNWVKI-ARSAAEVNDFTVANWLGPINWIQ 595

BLAST of CmoCh04G022770 vs. NCBI nr
Match: gi|778698533|ref|XP_004149498.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 911.0 bits (2353), Expect = 1.0e-261
Identity = 452/567 (79.72%), Postives = 504/567 (88.89%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKVV+SG+S ILVVGVALAVVA+VNK +D++ E   LSPKMKAVA ICS  DY  ECQ
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNK-SDSSDETANLSPKMKAVASICSNADYQPECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL +   NS+ +DPK+++KAAI +TIEE+ KG NLTD L VEAA+N TI+MSVDDCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAID LHAS STVGDPD+HT+ DR+ D+K WL+AVIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             LD AGKLTSNALAI+  V  IL++LGLQLKVQPSGRRLLGTTEV SD FPTWL G DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLAA+ G  RVKPN VVAKDGSGQ+KTI AALAAYPK L GRYVIYVKAGVY+E IIVT
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+NIFMYGDGPR TIVTGRKSNRDG+TTQ++ASF+AIG+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ +RQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTANGRAD ++ +GLVIHNCRIVPEQKLFA+RFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIMESTLGD IQP GYMPW+GNFAL TC Y EYGNRGPGA TNRRV+WKGVRVIGRNEAM
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           QFTAGPFL G  WL GTGGP+ +GLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CmoCh04G022770 vs. NCBI nr
Match: gi|659072605|ref|XP_008466391.1| (PREDICTED: pectinesterase-like isoform X3 [Cucumis melo])

HSP 1 Score: 898.7 bits (2321), Expect = 5.4e-258
Identity = 446/567 (78.66%), Postives = 503/567 (88.71%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+ KVV++ V+ +LVVGV LAV A+VNK N ++ E   +SPKMKAV+ ICS TDY +ECQ
Sbjct: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNK-NHSSDETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL     NS+ DDPK++LKAAI ATIEE+ KG NLTD L VEAA+N +I+M+VDDC+DL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAID LHAS STVGDPD+HT+ DRV D+K WL+AVIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           G LD A KLTSNALAI+D V  IL+ LGLQLKVQPSGRRLLGTTEV +DGFPTWL G DR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLAA+ G  RVKPNAVVAKDGSGQ+KTIAAALAAYPK L GRYVIYVKAG+Y+EYIIVT
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+N+FMYGDGPR TIVTGRKSNRDG+ TQ++A+F AIG+GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFVAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ  RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTANGRAD ++ +GLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIMESTLGD IQP GYMPW+GNFAL+TCLYLEYGNRGPGA+TNRRV+WKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           QFTAGPFLQG  WL  TGGP+ +GLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 565

BLAST of CmoCh04G022770 vs. NCBI nr
Match: gi|659072603|ref|XP_008466382.1| (PREDICTED: pectinesterase-like isoform X2 [Cucumis melo])

HSP 1 Score: 891.7 bits (2303), Expect = 6.6e-256
Identity = 441/567 (77.78%), Postives = 501/567 (88.36%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+ KVV++ V+ +LVVGV LAV A+VNK N ++ E   +SPKMKAV+ ICS TDY +ECQ
Sbjct: 1   MVAKVVMA-VTSLLVVGVVLAVFAMVNK-NHSSDETADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL     NS+ DDPK++LKAAI ATIEE+ KG NLTD L VEAA+N +I+M+VDDC+DL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQSAID LHAS STVGDPD+HT+ DRV D+K WL+AVIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
           G LD A KLTSNALAI+D V  IL+ LGLQLKVQPSGRRLLGTTEV +DGFPTWL G DR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLAA+ G  RVKPNAVVAKDGSGQ+KTIAAALAAYPK L GRYVIYVKAG+Y+EYIIVT
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+N+FMYGDGPR TIVTGRKSNRDG+ TQ++A+F+AIG+GFLCKSMGF+NTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ +RQFYRNCV+SGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTANGRAD ++ +GLVIHNCRIVPEQKLFA+RFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIME+TLGD IQP GYMPW+G+FAL TC Y EYGNRGPGA T RRV+WKGVRVIGRNEAM
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           QFTAGPFL G  WL  TGGP+ +GLKN
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of CmoCh04G022770 vs. NCBI nr
Match: gi|659072607|ref|XP_008466403.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 1.5e-255
Identity = 437/567 (77.07%), Postives = 501/567 (88.36%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+GKV+VS +S ILVVG+ALAVVA +NKRN +    + +SPKMKA++ ICS TDY +EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TL+  AQN++S+DPK Y KAAI+ATI+EITKG NL+D+L VEA +N +I+MSV+DC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQ AID L AS STVG+ D+HTDADR+ D+K WLSAV+S+QQSCLDGL E DP L+Q+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             LD AGKLTSNALAIL  V  IL+  GLQLKVQPSGRRLLGTTEV SDGFPTWL G DR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLA++ G  R  PNAVVAKDGSGQ+KTIAAALAAYPK L GRYVIYVKAG+YDEYI VT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+N+FMYGDGPR TIVTGRKSNRDG TTQ++ASF+AIG+GFLCKSMGF+NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDGHQDTLYVQ +RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTA GRA+ ++ TGLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIMESTLGD IQP GYMPW+GNFAL+TCLYLEYGNRGPGA TNRRV+WKGV+VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           Q+TAG FL G TWL  TGG +++GLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of CmoCh04G022770 vs. NCBI nr
Match: gi|659072601|ref|XP_008466374.1| (PREDICTED: pectinesterase-like isoform X1 [Cucumis melo])

HSP 1 Score: 888.3 bits (2294), Expect = 7.2e-255
Identity = 431/567 (76.01%), Postives = 497/567 (87.65%), Query Frame = 1

Query: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60
           M+ KV++ G++ ILVVGVALAVVA+V+    + A  + LSPKMKAV+ IC+  +Y +ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120
           +TLS  A N++SDDPK+Y++AAI++TIEE+ KG NLTD   +EAA+N +I+M V+DC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180
           LQ AID L AS STVG+PD+HT+ADRV D+K WL++VIS+QQSCLDGLEE DP L+QKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240
             L  AGKLTSNALAI+D V  IL   GLQLK QPSGRRLLGTTEVG DG+PTWL G DR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300
           KLLA+RGG  +VKPNAVVAKDGSGQFKTI+AALAAYPK L GRYVIYVKAG+Y+EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360
           K+M+NIFMYGDGP+ TIVTG KSNR G TTQD+A+F AIG+GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSD+SAFFNCRMDG+QDTLYVQ  RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480
           VRRPMDNQ+NTVTANGRAD ++ +GLVIHNCRIVPEQKLFA+RFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540
           VIMESTLGD IQP GYMPW+GNFAL+TCLYLEYGNRGPGA+TNRRV+WKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 568
           QFTAGPFLQG  WL  TGGP+ +GLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567