Cucsa.374150 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.374150 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 453.0 bits (1164), Expect = 4.9e-126
Identity = 228/561 (40.64%), Postives = 345/561 (61.50%), Query Frame = 1

Query: 5   VVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQTTLG 64
           + +S + LI +V VA+ V   +NK D   +  A ++  +KAV  +C+  DY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  SVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSA 124
             G N++  DP E +K A   T++++      +  +M E   ++  +M++D CK+L+  A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLD 184
           +DEL  S+  +G  + H  ++ + +++ WL+A IS++++CL+G +       + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLA 244
            A +LT N LAI+  +S+ + ++ +      + RRLL      ++ FP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 AKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMK 304
           A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y E + V K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DGITT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRP 424
           RVQSD S FFNCR DGYQDTLY  +HRQF+R+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME 484
           + NQ   +TA+GR D +E +G V   C I  E    A +     +LGRPWKEY+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTA 544
           + + DF+QP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + ++FT 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GPFLLGKAWLPGTGGPYLLGL 565
             ++ G  W+PG G PY  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cucsa.374150 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.4e-126
Identity = 228/563 (40.50%), Postives = 349/563 (61.99%), Query Frame = 1

Query: 4   KVVMSGISLILVVGVALAVV--AIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTT 63
           + V+  IS +L++ + +AV     VNKSD++ +   ++  +KA+  +C+  DY+  C+ T
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDE-EITTSVKAIKDVCAPTDYKETCEDT 74

Query: 64  LGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQ 123
           L     ++S  DP E +K A  +T++++      +   M+E   +   KM++D CK+L+ 
Sbjct: 75  LRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 134

Query: 124 SAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 183
            AI EL  S+  +G  + H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 194

Query: 184 LDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKL 243
           L  A +LT N LA+V  +S+ L ++ +    + + RRLL      S  FP+W+    R+L
Sbjct: 195 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 254

Query: 244 LAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKD 303
           L A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++K G+Y E + V + 
Sbjct: 255 LNAPMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAV 363
           M ++   GDGP KT+++G KS +DGITT  TA+ A +G+ F+ K++ F NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+RV +D S F+NC+ DGYQDTLY  +HRQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 483
           +P+ NQ   +TA+GR D +E +G V+  C IV E    A + +  T+LGRPWKEY+RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQF 543
           M + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E ++F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGKAWLPGTGGPYLLGL 565
           T   ++ G AW+PG G PY+LGL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Cucsa.374150 vs. Swiss-Prot
Match: PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 2.0e-124
Identity = 245/593 (41.32%), Postives = 358/593 (60.37%), Query Frame = 1

Query: 4   KVVMSGISLILV----VGVALAVVAIVNKSDSSDETANL------------SPKM----- 63
           ++++  +S+++V    +G A A V   NK+    +T N             SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVASICSNADYQPECQTTLGSVGNNSSVE-DPKEFIKAAITSTIEEMKKG 123
                    K + ++C++  Y+P CQ TL +     + + DP+  +K+AI +  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNW 183
           +    +L  E   N   K ++  CK L+  A +EL  S   + D +++     + D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKV 243
           L+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L  +    KV
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KV 263

Query: 244 QPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAAL 303
           +        ++  ++D   +WL+  +R++L A      +KPN  VAKDGSG + TI AAL
Sbjct: 264 KTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTTINAAL 323

Query: 304 AAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNT 363
            A P   +GRY IY+K G+Y+E +I+ K   N+ M GDG +KTIVTG KS+   I T  T
Sbjct: 324 KAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLT 383

Query: 364 ASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQF 423
           A+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+
Sbjct: 384 ATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQY 443

Query: 424 YRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRI 483
           YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G VIHNC +
Sbjct: 444 YRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTV 503

Query: 484 VPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPW-SGNFALATCSYFE 543
            P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   +FA+ T SY E
Sbjct: 504 APNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAE 563

Query: 544 YGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGL 565
           Y N GP   T  RV+W G RV+ + EAM+FT GPFL G+ W+   G P  LGL
Sbjct: 564 YKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Cucsa.374150 vs. Swiss-Prot
Match: PME4_ARATH (Pectinesterase 4 OS=Arabidopsis thaliana GN=PME4 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.0e-123
Identity = 257/602 (42.69%), Postives = 353/602 (58.64%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           M+GKVV+S  S++L+VGVA+ VVA +NK+      ANLSP+MKAV  IC +   +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGD----ANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATI--KMSVDDCKD 120
           TL  V +    EDP + IKA + +T +E+ K  N T    V   ++ +   K  +D CK 
Sbjct: 61  TLEPVKS----EDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           +   A+++L      +G+ DL     +I  +K WL  V +YQ  CLD +EE D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAIVGAVSDILKELGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V   + ++  ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTTEVDSDSFPTWLTGSDRKLLA--------AKRGGVRVKPNVVVAKDGSGQY 300
           G  RRLL   ++D    PTW++G+DRKL+A         + GG RV+ N VVAKDGSGQ+
Sbjct: 241 GPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKS--N 360
           KT+  A+ A P+  +GR +IY+KAG+Y E +I+ K   NIFM+GDG RKT+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 420
             G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEIS 480
           LYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      + 
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 -GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPWSGNF 540
            G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLL 565
              +C Y EY NRGPGA  NRRV W  V      E   FTA  +L    W+     P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of Cucsa.374150 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 2.1e-121
Identity = 234/566 (41.34%), Postives = 342/566 (60.42%), Query Frame = 1

Query: 4   KVVMSGISLILVVGVALAVVAIVNKSDSSDET---ANLSPKMKAVASICSNADYQPECQT 63
           K +++G+   L+V + +AV    +++ S  E      +     AV ++C+  DY+  C  
Sbjct: 11  KCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVN 70

Query: 64  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTD-NLMVEAANNATIKMSVDDCKDL 123
           +L     +S+   P + IK     TI  ++         L  +AAN+   K +++ C+ L
Sbjct: 71  SLMKASPDST--QPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKL 130

Query: 124 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 183
           +  A D+L           +   ED + D++ WL+  I+YQQ+C+D  EE + +L Q MQ
Sbjct: 131 MNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQ 190

Query: 184 DGLDVAGKLTSNALAIVGAVSDILKEL---GLQLKVQPSGRRLLGTTEVDSDSFPTWLTG 243
                + +LTSN LA++  +S++L E    G+   +    R+LL       D  P+W+  
Sbjct: 191 KIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA----EDGIPSWVGP 250

Query: 244 SDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPI 303
           + R+L+A K G   VK NVVVA DGSGQYKTI  AL A PKA +  +VIY+K GVYNE +
Sbjct: 251 NTRRLMATKGG---VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKV 310

Query: 304 IVTKDMKNIFMYGDGPRKTIVTGRKSNRDG-ITTQNTASFAAIGEGFLCKSMGFTNTAGP 363
            VTK M ++   GDGP KT +TG  +   G + T  TA+ A  G+ F  K++GF NTAGP
Sbjct: 311 DVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGP 370

Query: 364 EGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQN 423
           EGHQAVALRV +D + F+NC++DGYQDTLYV +HRQF+R+C +SGTVDFIFGD   ++QN
Sbjct: 371 EGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQN 430

Query: 424 SLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKE 483
             I+VR+PM +Q   +TA GR+DK+E +GLV+ NC I  E      +     +LGRPWKE
Sbjct: 431 CNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKE 490

Query: 484 YARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGR 543
           ++RT+IM +T+ D I PAG++PW+G+FAL T  Y EY N GPG+N  +RV+W G++ +  
Sbjct: 491 FSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSP 550

Query: 544 NEAMQFTAGPFLLGKAWLPGTGGPYL 562
            +A++FT   FL G  W+P    PY+
Sbjct: 551 KQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Cucsa.374150 vs. TrEMBL
Match: A0A0A0KLN2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G119700 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLGKAWLPGTGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Cucsa.374150 vs. TrEMBL
Match: A0A0A0KJ73_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G125700 PE=4 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 1.1e-270
Identity = 460/567 (81.13%), Postives = 516/567 (91.01%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60
           M+GKV++SG+SLILVVG+ALA+ A +NKS++S+  T ++SPKMKAV++ICS  DYQ EC+
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120
           TTL  V  N+S  DPK++ +AAI +TI E+ KGYNL+D+L+VEA+ NA+IKMSV+DCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DR+ADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240
           DGLDVAGKLTSNALAIV AVS+IL    LQLKVQPSGRRLLGTT VD D FPTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300
           KLLA+K+ GVR  PN VVAKDGSG+YKTI AALAAYPK L+GRYVIYVKAG+Y+E I +T
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGPRKTIVTGRKSNRDG TTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCV+SGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540
           VIMESTLGDFIQPAGYMPWSG+FAL TC Y EYGNRGPGANTNRRVRWKG +VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 567
           Q+TAG FLLG++WLP TGG Y LGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of Cucsa.374150 vs. TrEMBL
Match: W9QN86_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_013873 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 3.3e-198
Identity = 350/571 (61.30%), Postives = 438/571 (76.71%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANL---SPKMKAVASICSNADYQPE 60
           +VGKVV+S +S++L+VGVALAVV +VN   +     ++   S     V S+CSN DY+  
Sbjct: 3   IVGKVVVSVVSVLLLVGVALAVVVVVNNHGNGKHEGSVAAPSTSKSMVESVCSNTDYKTA 62

Query: 61  CQTTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNAT--IKMSVDD 120
           C+ TL  + +N +  D K+ IKAA+ +T++++ K  NL+++L+VEA NN +  +KM+++D
Sbjct: 63  CEQTLSPLSHNVTA-DYKDIIKAAVQATVDQVSKSLNLSESLLVEANNNNSTRLKMALED 122

Query: 121 CKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLK 180
           C DLL  AI +L AS+S VGD D+HT +DR A++++WL++VISY ++C+DG+EE  P+ +
Sbjct: 123 CNDLLSLAIGQLQASFSMVGDSDMHTIKDRSAELRSWLSSVISYAETCIDGVEE--PKFQ 182

Query: 181 QKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQP-SGRRLLGTTEVDSDSFPTWL 240
            +++  L  A  LT NALAIV  +S+IL   GL+L  +  +GRRLL  T + +D  PTW 
Sbjct: 183 GQLKGTLQNATALTDNALAIVSTISEILSAFGLKLNPKALNGRRLLSDTVIGNDGLPTWF 242

Query: 241 TGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNE 300
           + SDRKL+A   G  R+KP+ VVAKDGSGQ+KTI AALAAYPK   GRY+IYVKAGVYNE
Sbjct: 243 SASDRKLMAL-HGNGRIKPHAVVAKDGSGQFKTIAAALAAYPKNHVGRYIIYVKAGVYNE 302

Query: 301 PIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAG 360
            I V K+M NIFMYGDGPRKTIVTGRKS   G +T  TASF+ IG GF+CK MGF NTAG
Sbjct: 303 YITVEKNMVNIFMYGDGPRKTIVTGRKSYTGGFSTFRTASFSVIGAGFVCKGMGFQNTAG 362

Query: 361 PEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQ 420
           PEGHQAVALRVQS+ SAFFNCRMDGYQDTLYVQ  RQFYRNCVISGT+DFIFGD+  +IQ
Sbjct: 363 PEGHQAVALRVQSEMSAFFNCRMDGYQDTLYVQAGRQFYRNCVISGTIDFIFGDATVVIQ 422

Query: 421 NSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK 480
           NSL+IVRRPMDNQ N VTA GR ++ + +G+VIHNCRIVPEQKLFA+RFKIPT+LGRPWK
Sbjct: 423 NSLLIVRRPMDNQLNAVTAQGRKERHQPTGIVIHNCRIVPEQKLFADRFKIPTYLGRPWK 482

Query: 481 EYARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIG 540
           E++RTVIMESTL DFIQPAG+ PW+GNFAL T  Y EY NRGPGA TNRRV W+G  VIG
Sbjct: 483 EFSRTVIMESTLADFIQPAGWFPWAGNFALDTLYYAEYANRGPGARTNRRVHWRGYHVIG 542

Query: 541 RNEAMQFTAGPFLLGKAWLPGTGGPYLLGLK 566
           R+EA QFT G FL G+ WLP TG P+L   K
Sbjct: 543 RHEAQQFTVGQFLEGQRWLPATGAPFLPSFK 569

BLAST of Cucsa.374150 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.7e-197
Identity = 352/569 (61.86%), Postives = 432/569 (75.92%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNK--SDSSDETANLSPKMKAVASICSNADYQPEC 60
           M+GK V+SGISL LVVGV + +V++     SD +D   N+S  MKAVAS+C+ ADY+  C
Sbjct: 249 MIGKAVVSGISLFLVVGVIIGIVSVTRPHGSDRTDGDTNMSSSMKAVASVCATADYKDAC 308

Query: 61  QTTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKD 120
             TL  V  N S   PK++I+AA+  TI+++K   NL++ L  +A N++  +M++ DCKD
Sbjct: 309 MQTLSPVPKNGSSATPKDYIQAAVQVTIKQIKSSMNLSEKLF-QATNDSRTQMALGDCKD 368

Query: 121 LLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           LLQ AIDEL  S+S+VG+ DL T +    +I NWL+A +SYQQ+CLDG+ E  P+ +  M
Sbjct: 369 LLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAAVSYQQTCLDGVIE--PRFQAAM 428

Query: 181 QDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEV-DSDSFPTWLTGS 240
           Q GL  A +LTSNALAIV  +S IL +  + L ++P+ RRLLG  EV   D +PTW + +
Sbjct: 429 QKGLLNATQLTSNALAIVSDLSQILTKFNVPLDLKPNSRRLLGEIEVLGHDGYPTWFSAT 488

Query: 241 DRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPII 300
           DRKLLA +  G R+ PN +VAKDGSG + TI AALAAYPK LKGRYVIYVKAG+Y E I 
Sbjct: 489 DRKLLALQDNG-RLTPNAIVAKDGSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYIT 548

Query: 301 VTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEG 360
           VTKD  N++MYGDGPRKTIVTG K  RDGITT  TA+F+AIG+GF+ +SMGF NTAGP+G
Sbjct: 549 VTKDHVNVYMYGDGPRKTIVTGTKCYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDG 608

Query: 361 HQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSL 420
           HQAVALRVQSD SAFFNCRMDGYQDTLYVQ HRQFYRNCVISGT+DFIFGDS T+IQNSL
Sbjct: 609 HQAVALRVQSDMSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTIDFIFGDSTTVIQNSL 668

Query: 421 IIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYA 480
           IIVRRP D QQNTVTA G+ +K+E +GLVIH+CRIVPEQKLF +RFKIP+FLGRPWK Y+
Sbjct: 669 IIVRRPNDKQQNTVTAQGKTEKRETTGLVIHDCRIVPEQKLFPDRFKIPSFLGRPWKPYS 728

Query: 481 RTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVI-GRN 540
           +T+IME+TLGDFIQPAG+ PW+G F   T  Y EYGN GPGANT+ RV WKG R+I  RN
Sbjct: 729 KTIIMETTLGDFIQPAGWTPWAGKFVPNTLLYAEYGNLGPGANTHSRVTWKGYRIIKTRN 788

Query: 541 EAMQFTAGPFLLGKAWLPGTGGPYLLGLK 566
           EA+Q+T   F+ G  WL     PYL GLK
Sbjct: 789 EALQYTVNSFIQGNLWLKQINIPYLPGLK 813

BLAST of Cucsa.374150 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 3.2e-108
Identity = 192/257 (74.71%), Postives = 221/257 (85.99%), Query Frame = 1

Query: 307 MYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQ 366
           MYGDGPRKTIVTG KS RDGITT  TA+F+AIG+GF+ +SMGF NTAGP+GHQAVALRVQ
Sbjct: 1   MYGDGPRKTIVTGTKSYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDGHQAVALRVQ 60

Query: 367 SDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDN 426
           SD SA FNCRMDGYQDTLY+Q HRQFYRNCVISGT+DFIFGDS T+IQNSLIIVRRP DN
Sbjct: 61  SDMSAIFNCRMDGYQDTLYIQAHRQFYRNCVISGTIDFIFGDSTTVIQNSLIIVRRPKDN 120

Query: 427 QQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTL 486
           QQNTVTA+G+A+K+E +GLVIHNCRIVPEQKLF +RFKIP+FLGRPWK Y++T+IME+TL
Sbjct: 121 QQNTVTAHGKAEKRETTGLVIHNCRIVPEQKLFPDRFKIPSFLGRPWKPYSKTIIMETTL 180

Query: 487 GDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVI-GRNEAMQFT--- 546
           GDFIQPAG+MPW+G+FAL T  Y EYGNRGPGANT  RV WKG R+I  RNEA+Q+T   
Sbjct: 181 GDFIQPAGWMPWAGDFALNTLFYAEYGNRGPGANTRSRVTWKGYRIIKTRNEALQYTLAE 240

Query: 547 ----AGPFLLGKAWLPG 556
                G  ++GKA + G
Sbjct: 241 RGINKGTKMIGKAVVSG 257

HSP 2 Score: 697.2 bits (1798), Expect = 1.7e-197
Identity = 352/569 (61.86%), Postives = 437/569 (76.80%), Query Frame = 1

Query: 3   GKVVMSGISLILVVGVALAVVAIVN-----KSDSSDETANLSPKMKAVASICSNADYQPE 62
           GKV + GIS+ILVVGVA+ ++A ++     KS   D+   LS   KAVA+ICS  DY+ +
Sbjct: 4   GKVAVGGISIILVVGVAIGLIAGLHGRGGHKSTDGDDEKVLSTTTKAVAAICSPTDYKQQ 63

Query: 63  CQTTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCK 122
           C ++  ++ NN S   PK+F+ AAI  T++E+K+    ++++ +   ++   KM+ +DC+
Sbjct: 64  CISSFQTLANNHSAT-PKDFLMAAIDITMKEVKEAIGKSESIGLAGNSSGRQKMATEDCQ 123

Query: 123 DLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQK 182
           DLLQ AI EL AS+S VGD  +HT  DR  D+KNWL+AVISYQQSC+DG +E  P++K  
Sbjct: 124 DLLQLAIGELQASFSMVGDAAMHTINDRAQDLKNWLSAVISYQQSCMDGFDE-TPEVKSA 183

Query: 183 MQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGS 242
           +Q+GL  A +LTSNALAIV  +S IL    + L    S RRL   TE +SD +P+W + +
Sbjct: 184 IQNGLLNATQLTSNALAIVSEISAILTSFNIPLNFTSSFRRLQEATE-ESDQYPSWFSAA 243

Query: 243 DRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPII 302
           DRKLL     G +V PN VVA DGSGQYK+IGAALAAYPK L GRYVIYVKAG+Y+E I 
Sbjct: 244 DRKLLGRVDNG-KVTPNAVVALDGSGQYKSIGAALAAYPKNLNGRYVIYVKAGIYDEYIT 303

Query: 303 VTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEG 362
           + K++ NIFMYGDGPRKT+VTG+KS  DGITT  T++F+ IG GF+CKSMGF NTAGPEG
Sbjct: 304 IEKNLVNIFMYGDGPRKTMVTGKKSFLDGITTYKTSTFSVIGNGFICKSMGFRNTAGPEG 363

Query: 363 HQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSL 422
           HQAVALRVQSD SAFFNCRMDGYQDTLYVQ HRQFYRNCVISGTVDFIFGDS T+IQNSL
Sbjct: 364 HQAVALRVQSDYSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTVDFIFGDSTTLIQNSL 423

Query: 423 IIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYA 482
           IIVR+P+DNQQNT+TA+GRA+K+E +GLVI NCRIVPE+KLF  RFK P++LGRPWK Y+
Sbjct: 424 IIVRKPLDNQQNTITAHGRAEKRETTGLVIQNCRIVPEEKLFPTRFKTPSYLGRPWKAYS 483

Query: 483 RTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVI-GRN 542
           RTV+MEST+GDFI PAG++PW GNFAL T  Y EY N+GPGANT +RV+WKG +VI  RN
Sbjct: 484 RTVVMESTMGDFIHPAGWLPWMGNFALDTLYYAEYANKGPGANTAQRVKWKGYKVITNRN 543

Query: 543 EAMQFTAGPFLLGKAWLPGTGGPYLLGLK 566
           EA+QFTAGPF+ G  WL  TG PY LG +
Sbjct: 544 EALQFTAGPFIQGNEWLRLTGAPYFLGFR 568

BLAST of Cucsa.374150 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 453.0 bits (1164), Expect = 2.7e-127
Identity = 228/561 (40.64%), Postives = 345/561 (61.50%), Query Frame = 1

Query: 5   VVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQTTLG 64
           + +S + LI +V VA+ V   +NK D   +  A ++  +KAV  +C+  DY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  SVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQSA 124
             G N++  DP E +K A   T++++      +  +M E   ++  +M++D CK+L+  A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQTIM-ELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDGLD 184
           +DEL  S+  +G  + H  ++ + +++ WL+A IS++++CL+G +       + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKLLA 244
            A +LT N LAI+  +S+ + ++ +      + RRLL      ++ FP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 AKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMK 304
           A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++KAG+Y E + V K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DGITT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRP 424
           RVQSD S FFNCR DGYQDTLY  +HRQF+R+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIME 484
           + NQ   +TA+GR D +E +G V   C I  E    A +     +LGRPWKEY+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTA 544
           + + DF+QP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + ++FT 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GPFLLGKAWLPGTGGPYLLGL 565
             ++ G  W+PG G PY  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of Cucsa.374150 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 452.6 bits (1163), Expect = 3.6e-127
Identity = 228/563 (40.50%), Postives = 349/563 (61.99%), Query Frame = 1

Query: 4   KVVMSGISLILVVGVALAVV--AIVNKSDSSDETANLSPKMKAVASICSNADYQPECQTT 63
           + V+  IS +L++ + +AV     VNKSD++ +   ++  +KA+  +C+  DY+  C+ T
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDE-EITTSVKAIKDVCAPTDYKETCEDT 74

Query: 64  LGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLLQ 123
           L     ++S  DP E +K A  +T++++      +   M+E   +   KM++D CK+L+ 
Sbjct: 75  LRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 134

Query: 124 SAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQDG 183
            AI EL  S+  +G  + H  ++ +  ++ WL+A IS++Q+CLDG +       + ++  
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 194

Query: 184 LDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRKL 243
           L  A +LT N LA+V  +S+ L ++ +    + + RRLL      S  FP+W+    R+L
Sbjct: 195 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 254

Query: 244 LAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKD 303
           L A      VKP++VVA+DGSGQYKTI  AL   PK     +V+++K G+Y E + V + 
Sbjct: 255 LNAPMS--EVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAV 363
           M ++   GDGP KT+++G KS +DGITT  TA+ A +G+ F+ K++ F NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+RV +D S F+NC+ DGYQDTLY  +HRQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVI 483
           +P+ NQ   +TA+GR D +E +G V+  C IV E    A + +  T+LGRPWKEY+RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQF 543
           M + + DF+ P G+ PW G F L T  Y E  N GPGA   +RV W G++ +   E ++F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGKAWLPGTGGPYLLGL 565
           T   ++ G AW+PG G PY+LGL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Cucsa.374150 vs. TAIR10
Match: AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-125
Identity = 245/593 (41.32%), Postives = 358/593 (60.37%), Query Frame = 1

Query: 4   KVVMSGISLILV----VGVALAVVAIVNKSDSSDETANL------------SPKM----- 63
           ++++  +S+++V    +G A A V   NK+    +T N             SPK      
Sbjct: 24  RIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSAA 83

Query: 64  ---------KAVASICSNADYQPECQTTLGSVGNNSSVE-DPKEFIKAAITSTIEEMKKG 123
                    K + ++C++  Y+P CQ TL +     + + DP+  +K+AI +  +++ + 
Sbjct: 84  QTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQV 143

Query: 124 YNLTDNLMVEAANNATIKMSVDDCKDLLQSAIDELHASYSTVGDPDLHTNEDRIADIKNW 183
           +    +L  E   N   K ++  CK L+  A +EL  S   + D +++     + D+ +W
Sbjct: 144 FKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSW 203

Query: 184 LTAVISYQQSCLDGLEEFDPQLKQKMQDGLDVAGKLTSNALAIVGAVSDILKELGLQLKV 243
           L+AV+SYQ++C+DG EE   +LK +++   + +  LTSN+LA++ ++   L  +    KV
Sbjct: 204 LSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVP---KV 263

Query: 244 QPSGRRLLGTTEVDSDSFPTWLTGSDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAAL 303
           +        ++  ++D   +WL+  +R++L A      +KPN  VAKDGSG + TI AAL
Sbjct: 264 KTRLLLEARSSAKETDHITSWLSNKERRMLKAVDVKA-LKPNATVAKDGSGNFTTINAAL 323

Query: 304 AAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNT 363
            A P   +GRY IY+K G+Y+E +I+ K   N+ M GDG +KTIVTG KS+   I T  T
Sbjct: 324 KAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLT 383

Query: 364 ASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQF 423
           A+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +GYQDTLY  THRQ+
Sbjct: 384 ATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQY 443

Query: 424 YRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRI 483
           YR+CVI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR DK + +G VIHNC +
Sbjct: 444 YRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTV 503

Query: 484 VPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPW-SGNFALATCSYFE 543
            P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   +FA+ T SY E
Sbjct: 504 APNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAE 563

Query: 544 YGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLLGL 565
           Y N GP   T  RV+W G RV+ + EAM+FT GPFL G+ W+   G P  LGL
Sbjct: 564 YKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Cucsa.374150 vs. TAIR10
Match: AT2G47030.1 (AT2G47030.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 443.7 bits (1140), Expect = 1.7e-124
Identity = 257/602 (42.69%), Postives = 353/602 (58.64%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           M+GKVV+S  S++L+VGVA+ VVA +NK+      ANLSP+MKAV  IC +   +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNGD----ANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATI--KMSVDDCKD 120
           TL  V +    EDP + IKA + +T +E+ K  N T    V   ++ +   K  +D CK 
Sbjct: 61  TLEPVKS----EDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKM 180
           +   A+++L      +G+ DL     +I  +K WL  V +YQ  CLD +EE D  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAIVGAVSDILKELGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V   + ++  ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTTEVDSDSFPTWLTGSDRKLLA--------AKRGGVRVKPNVVVAKDGSGQY 300
           G  RRLL   ++D    PTW++G+DRKL+A         + GG RV+ N VVAKDGSGQ+
Sbjct: 241 GPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTKDMKNIFMYGDGPRKTIVTGRKS--N 360
           KT+  A+ A P+  +GR +IY+KAG+Y E +I+ K   NIFM+GDG RKT+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGYQDT 420
             G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRADKKEIS 480
           LYV   RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G      + 
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 -GLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTVIMESTLGDFIQPAGYMPWSGNF 540
            G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQFTAGPFLLGKAWLPGTGGPYLL 565
              +C Y EY NRGPGA  NRRV W  V      E   FTA  +L    W+     P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of Cucsa.374150 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 234/566 (41.34%), Postives = 342/566 (60.42%), Query Frame = 1

Query: 4   KVVMSGISLILVVGVALAVVAIVNKSDSSDET---ANLSPKMKAVASICSNADYQPECQT 63
           K +++G+   L+V + +AV    +++ S  E      +     AV ++C+  DY+  C  
Sbjct: 11  KCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVN 70

Query: 64  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTD-NLMVEAANNATIKMSVDDCKDL 123
           +L     +S+   P + IK     TI  ++         L  +AAN+   K +++ C+ L
Sbjct: 71  SLMKASPDST--QPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKL 130

Query: 124 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 183
           +  A D+L           +   ED + D++ WL+  I+YQQ+C+D  EE + +L Q MQ
Sbjct: 131 MNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQ 190

Query: 184 DGLDVAGKLTSNALAIVGAVSDILKEL---GLQLKVQPSGRRLLGTTEVDSDSFPTWLTG 243
                + +LTSN LA++  +S++L E    G+   +    R+LL       D  P+W+  
Sbjct: 191 KIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA----EDGIPSWVGP 250

Query: 244 SDRKLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPI 303
           + R+L+A K G   VK NVVVA DGSGQYKTI  AL A PKA +  +VIY+K GVYNE +
Sbjct: 251 NTRRLMATKGG---VKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKV 310

Query: 304 IVTKDMKNIFMYGDGPRKTIVTGRKSNRDG-ITTQNTASFAAIGEGFLCKSMGFTNTAGP 363
            VTK M ++   GDGP KT +TG  +   G + T  TA+ A  G+ F  K++GF NTAGP
Sbjct: 311 DVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGP 370

Query: 364 EGHQAVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQN 423
           EGHQAVALRV +D + F+NC++DGYQDTLYV +HRQF+R+C +SGTVDFIFGD   ++QN
Sbjct: 371 EGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQN 430

Query: 424 SLIIVRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKE 483
             I+VR+PM +Q   +TA GR+DK+E +GLV+ NC I  E      +     +LGRPWKE
Sbjct: 431 CNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKE 490

Query: 484 YARTVIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGR 543
           ++RT+IM +T+ D I PAG++PW+G+FAL T  Y EY N GPG+N  +RV+W G++ +  
Sbjct: 491 FSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSP 550

Query: 544 NEAMQFTAGPFLLGKAWLPGTGGPYL 562
            +A++FT   FL G  W+P    PY+
Sbjct: 551 KQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of Cucsa.374150 vs. NCBI nr
Match: gi|778698533|ref|XP_004149498.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLGKAWLPGTGGPYLLGLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Cucsa.374150 vs. NCBI nr
Match: gi|659072603|ref|XP_008466382.1| (PREDICTED: pectinesterase-like isoform X2 [Cucumis melo])

HSP 1 Score: 1055.0 bits (2727), Expect = 4.5e-305
Identity = 522/566 (92.23%), Postives = 546/566 (96.47%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MV KVVM+  SL LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQT
Sbjct: 1   MVAKVVMAVTSL-LVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLG+VG+NSSV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVA KLTSNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D FPTWLTG+DRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRKSNRDGI TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCV+SGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IME+TLGDFIQPAGYMPWSG+FALATCSYFEYGNRGPGANT RRVRWKGVRVIGRNEAMQ
Sbjct: 481 IMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFLLG+AWLP TGGPYLLGLKN
Sbjct: 541 FTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of Cucsa.374150 vs. NCBI nr
Match: gi|659072605|ref|XP_008466391.1| (PREDICTED: pectinesterase-like isoform X3 [Cucumis melo])

HSP 1 Score: 1030.0 bits (2662), Expect = 1.5e-297
Identity = 511/566 (90.28%), Postives = 536/566 (94.70%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MV KVVM+  SL LVVGV LAV A+VNK+ SSDETA++SPKMKAV+SICSN DYQ ECQT
Sbjct: 1   MVAKVVMAVTSL-LVVGVVLAVFAMVNKNHSSDETADMSPKMKAVSSICSNTDYQQECQT 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TLG+VG+NSSV+DPKEF+KAAIT+TIEEMKKGYNLTDNLMVEAANNA+IKM+VDDC+DLL
Sbjct: 61  TLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           QSAIDELHASYSTVGDPDLHTNEDR+ADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQG 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GLDVA KLTSNALAIV AVSDILK LGLQLKVQPSGRRLLGTTEVD+D FPTWLTG+DRK
Sbjct: 181 GLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLAAKRGGVRVKPN VVAKDGSGQYKTI AALAAYPKALKGRYVIYVKAG+YNE IIVTK
Sbjct: 241 LLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVTK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKN+FMYGDGPRKTIVTGRKSNRDGI TQNTA+F AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFVAIGEGFLCKSMGFQNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YARTV
Sbjct: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAMQ
Sbjct: 481 IMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAGPFL G+AWLP TGGPYLLGLK+
Sbjct: 541 FTAGPFLQGRAWLPTTGGPYLLGLKS 565

BLAST of Cucsa.374150 vs. NCBI nr
Match: gi|659072601|ref|XP_008466374.1| (PREDICTED: pectinesterase-like isoform X1 [Cucumis melo])

HSP 1 Score: 961.4 bits (2484), Expect = 6.7e-277
Identity = 471/567 (83.07%), Postives = 515/567 (90.83%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60
           MV KV++ GI+LILVVGVALAVVAIV+ + SSD  T +LSPKMKAV++IC+NA+YQ ECQ
Sbjct: 1   MVNKVILPGITLILVVGVALAVVAIVHSNKSSDAATEDLSPKMKAVSTICANANYQQECQ 60

Query: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120
            TL +  +N+S +DPKE+++AAI STIEE+KKGYNLTD  M+EAANN +IKM V+DC+DL
Sbjct: 61  NTLSNAAHNASSDDPKEYVRAAILSTIEEVKKGYNLTDGFMIEAANNRSIKMGVEDCRDL 120

Query: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180
           LQ AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQLKQKMQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240
           DGL  AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEV  D +PTWLTG+DR
Sbjct: 181 DGLSDAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVGKDGYPTWLTGADR 240

Query: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300
           KLLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPKAL+GRYVIYVKAG+YNE + +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKALRGRYVIYVKAGIYNEYVHIT 300

Query: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480
           VRRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540
           VIMESTLGDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAM
Sbjct: 481 VIMESTLGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 567
           QFTAGPFL G+AWLP TGGPYLLGLK+
Sbjct: 541 QFTAGPFLQGRAWLPTTGGPYLLGLKS 567

BLAST of Cucsa.374150 vs. NCBI nr
Match: gi|449467649|ref|XP_004151535.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 959.5 bits (2479), Expect = 2.6e-276
Identity = 467/566 (82.51%), Postives = 513/566 (90.64%), Query Frame = 1

Query: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60
           MV KVV+ GISL+LVVGVALAVVAIVN + SSD T +LSPKMKAV++ICS A+YQ ECQ 
Sbjct: 1   MVNKVVLPGISLLLVVGVALAVVAIVNSNKSSDATEDLSPKMKAVSTICSTANYQEECQN 60

Query: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120
           TL +  +N+S +DPKE++KAAI +TI+E+KKGYNLTD  ++EAANN +IKM V+DC+DLL
Sbjct: 61  TLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDLL 120

Query: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180
           Q AID+L ASYSTVG+PDLHTN DR+ADIKNWLT+VISYQQSCLDGLEEFDPQL+QKMQD
Sbjct: 121 QFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQD 180

Query: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240
           GL+ AGKLTSNALAIV AVSDIL   GLQLK QPSGRRLLGTTEVD+D +PTWLTG+DRK
Sbjct: 181 GLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADRK 240

Query: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300
           LLA++ GG +VKPN VVAKDGSGQ+KTI AALAAYPK L+GRYVIYVKAG+Y E + +TK
Sbjct: 241 LLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHITK 300

Query: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360
           DMKNIFMYGDGP+KTIVTG KSNR G TTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQA 360

Query: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALRVQSDRSAFFNCRMDGYQDTLYVQT RQFYRNCVISGTVDFIFGDS T+IQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480
           RRPMDNQQNTVTANGRAD KE+SGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540
           IMEST+GDFIQP GYMPWSGNFAL TC Y EYGNRGPGA TNRRVRWKGV+VIGRNEAMQ
Sbjct: 481 IMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 567
           FTAG FL GK WLP TGGPYLLGLK+
Sbjct: 541 FTAGSFLQGKTWLPTTGGPYLLGLKS 566