Lsi11G000210 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi11G000210
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Thiamine-phosphate synthase
Location	chr11 : 231452 .. 238444 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGTCTTAGGATTTTGCAAACTAGTTTCTACAAGAGAGACACAACATGCAATCAGACTTGAATCATTGGTCATCCTAATCAAAACATACATCCAAGAAATTCTAAGTTATTCAATCCTACATTCTTCTAGGGCAAAAATAAAATTAAAAAAAAAAAAAAATCAAATTTGTTTTTAACCACTCTTCTAGCTGTCCTTTTTCAAAACCCTATTTCACGTTCAGCCGCCACCTACCCACCAGTCATTTCACTCCGGCCCGAACCGCCCGCCGTTCCTGCCCGCCACCGACTTAACGGTGCCGCCGCATGCTGCACTCTCAGCTGCTCCAGTCTCAGCCTTCCGACGAAATTGCTGCCGCCTCCTACCTCTCACACTCTCTCGCCTCTCCGAGCAATCAGAGTTACATATCTCTGCTTTGTTTCTCAGAAACACAGTGATTCAGCCCCTAACCGGCGCCGTTGGTGAGATGGTGCCGCTGCCCCTTATTTCTCAGATTCCCAAGGTCTTATTATTATTTTTTTAATTATATATATATATATATATATATATATATATATATATTTAATATTGTTTCGTTGGGTTATGTTTGTTTATCCACGGATAAGAAAATCAAGAGTAGTCCTTTATTTATTTATTGTTTATTTCAAATTTCTTCGTCAACCAATGTTAAGCAAGTCTAAATGAAAACATCAAACTAATAATGCATATCTCACAAACTAGAAAGAACAGAACATATTGAACGGAACAAAAAAAAAACATTTCAATTATTGTCTGAATCAATAATTAAAAAGGATAAATAATGTTTAAGAATGAACAAAGCGAAGAAAAAATCCTGTAGTTTTGTTGGCTATACGAAACTTGTTGGAATTGAAAATTTTCTTATCAATTAAAAAACCCACCAAAATACAATGCATAAACAAAATAGAAAACTTAAATTTGAGTTTAGTTTGATTTAATGGCATTAAATCTTGTTGATTATAGAAGAAACTATCCTGGCATATGGAGGTGGCTAGGCAGATATTATTATTTTTATTATTTTAAAAATCTCTTATGGAGTAGAATTGGAAACATAATTTTTAAAGTTTAAAATTCTCTCAAATCAGACCAATGTAGAACAATTTATTTATTTTATTTTTAAATAAAACAAGATTTCAAATCACTACTGATTTCAAAATTCCTGCCCTTTTTTTATGCATCCAAAATTGATTTGAAATCCTTCTACATTCAGGCGTTCTAAGCAGGGAGTTAGCTTTATCTATTATGAAAATATAATTTATAATTTATGGGTTTTCCTGACTGTGCCTTAATTTGTACTGTTGTCTATTTTAGTTCAGTCAAGTTTCTAGATTTTGTGTGGCCATGAAGAAGCAGGAAGAAACCGTTGTAGCGTCAAGTAATCGCTATGAGATGAGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGCTCCACTGTGATAACTGCTATTACTGCACAGAACACTGTGGGGGTTCAGGTCATTTTCACACACTCATAATTATTATCTATATTTATGTATTGATGTCTTTATTTTGGGTTGAAAGAACAAAGGGAAGGTCTGGTGAATAGAGAAATAAACTTTCACTTGCTACTTGTCTGGCTGTTTTGGTATAATGGGTTGTAGAAAATGGTTTAACATGCTGCAATGGTCAATTCTAATGTTATTGAGAGTTTGGGATGATTTTTGAAAGCAAGGTGCTTTTGAGTGAAAGCTCATCCTTTGTTAGCACTTTATTAAGTGTAACACAAAATTTATTTAACACTAAGGTGTAATAACTTTTTCAGGATGTAAACATAGTGCCGGAGGGTTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAGGTGGATGTGGTGAGTATAATTAAATTAGGATTGGGAATAAGCCCTTCGTAGATCCAACAGTATATTTATATTTTTTTTAATAAGAAATTTTAAGTTAGGATTGCCTAATGAAAGTTCTTTAGGAGCTCATGCGAACAAGGTTGTGGACTGTTCATGTATAGATTGCACAGGCTAGCTGTTATTGTTCTTTCAGAGTACATACTAATATGTACTTTATGTAAGTTAGGAAGATTGCACGGATAGAAGATTACTAAATGATTACATTAATTTAGGTGAAAACAGGAATGCTACCTTCTACTGGCATTGTTCGGGTTCTACATCAGTGCCTGAAGGAGTTTCCCATTCGAGGTATAAAGTAATTCAGGAAGTTATTGGTGGATTTCTTTGATTAAATAATCGTGGTTTAAAATTTTAATGTAATTAAAGGCAGCCTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGAGATGTTCTGGCTGGTCCTACCATTATTTCAGTGTTACAGTAAGTTGATTCTCTCTTTAAAGGGAATATTTTCAGTCCTTCAAGCTGGTTCTATTTGTATGATTGTGTTAATTTCTTGCGCTTCATGTTTCATTTTTTATATATCTTCATAATTAGTACTTTCTGGTTTAAAAGGCTTCTGAAGCTTATGTGTGGAGCTCAAACTTTTAGCATCAAAGCTTACATCCGACGCACCTTTATTAGAACCTATTGAGGCTTGAAATCCTTATGCCTCACACAATAATTTGAAAAATTATACTATGACACTTCTGAAGCTCGTGTTTGATCTTCATATGTTAGTTTTTCATAAATCCATGACAATTCTTTTGCTTGCAGGGAAGAGCTTCTACCAATGGCTGACTTGGTAACTCCAAATTTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACATTAATCCATCAGATGGGATCAAAGTAAGTTTTTAATCCATCAGATAAGCATGTCTTCTTTTGTAATTTCATTTAATCCATCAGATTAGATTGTCTTTTGTAATTTCATTTAATCCATCAGATAAGCTTCTCTTCTTTTGTAATTTCATTTTATCAATGATGTGAAGGAATCCATGTTTCTCATAATAAAAAAAAGGAAAATATTCTTTTCTGTTACTATTGGTTTCAGAGACTGACTTTGGCGCTCAGTTTTCCTCATTGCTGCTTGTCTCCTAGCTGTAATTTTAGTACCATTTTGTTAATCGAAATAATTTTTTTTGGCAGGTGCTTCTATTCTTTCAAAAGTTGCACCTTTCATAAACTTTTCAAAACTACTTTAGGAGTACAAATTCTTTAAAATTTTGGATAAAAAATTAGGACATGAAATAGTGTGGTGATGACCTAGAATAAAAATTGGATTGTAATATTGCAACTTTTGAAAGTATAGCGGTACTTTTGAAACAAAGAATAAATTTTAGGGATGTCTCTTATAATTTAACTTCTAATTCTTTGGTTGTTTGGATTAACAACAAAGATACAATAAGACTTGTCAGTATAGTTTTCTGCAAGAATTTTGTCAAGAATATTATCAAGATAGATACTAGAAATTCTATTAGGAGGCATATTGTTGGTCATTTGTACCTAGTCAAGTAATTTTCTAGTAAGAGAGTGTGTAATAAATCATCATGGACTGGTCGGTTGTAAGAGTTGTGGAGGAATAGGTTCAAACGTTCTTTAGCTATCTATCTTGAATATTAAAATCTTACTAGTTTTCTAACAACAAAATGTTATAATTGTGTTCGTGAAATTAGTTGAGGTGTACACCAATTAGGTTAGACACACAATCAATAGATATCAAAGAAAGGAAGGAAAAAAAAGATAGAGAGTTATAGAAAGAAATTTTTGAAAGGATGAAACAAAGATTTATTTTATTTCTTTATCTATTTTTGGATAAGAAATAATTTTATGATGAATGAAAACAAAAAGATAGGGAGTTATAAAAAGAGTGACGGAGGGAGGTTGAGAGAAGCAAATGTTTAGTTGTTTCTTCTTTTTCTCCTTTGATTAATTTCTTTTTTCCTAATTTCTTATTCTGCAAGTTTTTTTTTTTTTTTTGAAAATGAAATCAGGACTTTTCACTAAGTAAATGAAAAGAGGCTAATGCTGAAAGATACCAGAAAGATAATTTTAATTTGCCAACATGAGCTTGACTCATCAGTAATTGGCGTGTACTCCCTTGTCGGAGGTTCAATCCCATCCCACAAGTGTTATATTCAAATAATAATAATAATAATCTCGAATTCAATTTTCTACCCAATGAAAATCAGCCTCGCACATTTTAGATGCCTCAGTTTCATAGTGTCATTTTTCGGTTGTAAGGTTGTAGAAGATTTCGTTATTGATACAAATCATGACCATTGAGTTGCCATTAATAAACTTTTGTTGTCTTTAATTAATTTCTAGCTTTTAAGTGTTAAACTTAAGCTCAATTTAGAACAAAATTTTCGAATTCTTGCATCTAGATCTTCGATTTAAGAGGTAATCTAATTCTAACATTTTCTTTTGGTTGATCTAAATTTGTATAAGGTGGTGTTAATTTCTTAGTTTCAAAAGTTTTTGCTTCAACAAAAGTTTGGATCTTTGGGTTGAGTATTAGGATTGCGTTCTAAAGACAATATCCATTGCAGGCATTTGATTTTATGGTTTTATATTTATCAAACTTTAACCTACTCGTGAGTGATGCTAAATAGCATAATGTTGTTTCCAGGAATGTACTTGTCAAAGGTGGGGACCTTCCCGATTCCTTGGATGCTGTCGATATATTCTTTGATGGTAAGTACATATTGCATAATTCTGACAATTGGATATTATTTTTTGTTATGAAGGAAACAGATCCATTGAATCCAAAATTTGAACTAATTTTCACAGGCAAGGATTTGCATGAGCTACGATCTTCGCGCATAAAGACTCGCAACACTCATGGTACTGGATGCAGCTTAGCATCATGCATAGCAGCTGAACTCGCTAAAGGGTCTTCAATGTTCTCAGCGGTTAAGGTATTTGTCTATGGATCTCTTCTTTAATAACTCCCGCCATAAGAAGATTCAAGATAGGACATGTTTAAATTGGAATGCTTTCATTTCTTCAAAATAATTTATCTATCTTTGTACTTTATTTGAGTTATTTGACATCTTGTATTTTCGAGCATCAGACTCTTTTCAGTAAATCAATGAAAAGTTCCGTTTCTCAAAGAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAAATTAATTTCAAGTCTCAAGGGGGGCTCATAATAGAAGTTCCTACTGAAAAATAAATTAATGACTAAAGACTTATGAACCATTTTCAATTTGTTGTAGGATTATAATTGTTTAAAATAATGCTATATTCACTATGTACGCTCACTTACCCTAATGGGTGGTTAAGGATTGGGATGTTTGGAAATACCTCCGGTCTTGAGTTCAACCCTGCAAGTCCACAACTAAGGTTTTATTTATCTGGATCTTTACAGGCAAGCAAACAGTTCATTGAAAGAGCATTGAAGTACAGCAAGGACATCAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTCAAGAGTCGAGAACAAAGTTCCTATAGACAGGGTTGTTTCAATCCAGCTGACTTATTCTTGTATGCTGTTACAGACTCAGGTATGAATAAGCATTGGGACCGTTCTATCACCGATGCTGTTAAAGCTGCAGTGGAAGGAGGTGCTACTATTGTTCAAATAAGGTTTGTCTAATGTCTTCCATATATCAATTTGTTATTTTGTTCGTTAAAAATGCTCTTCACAAGTTGGTAGATAATTCAAGTCATTACTTCAAATGTATGGAGTTCCTTGTTGCATTTTAGTATCCTCTCAACACCTCAGTTCAAAGGTTAAATGGCTTTAGAACTTACACATAAAGTGCAGGCTGAATGCTTCCTTAGTCTAGCGGTAGTGAAGTAGTTCTCACTCGACTGTAACGATTTGAATATCTTAGGCCTTGTTTGGTAACCATTTGGTTTTTAATTTTTGAAAATTAAGCCCATGAATATACTTTTTATAAGCTAATTTTTGAAAACTAAAAAAAATAGTTCTTAAAAACTTGTTTTTGTATTTGAAATTTGGCTAAGAATCCAACTCGTTTACTTAAGAAAGATGAAACTATGGTAAGAAGTTGAGAAGAAATATGTTTAATTTTAAAAAACTGAATGGTTACCAAATGAGGTCTTTCTTAAATAACTTTGTAAGAGGTTAAATACTTGGCATAGTAATGGAAACTTGTATTCAATTGTTAATTTTCTTCCAATGATTAATGTTCTCTACTTCTCTTACTTCTACGAACAGGGAAAAAGATGCTAAAACTTGCGATTTCTTGGAAGTAGCAAAGTCATGTATGAAGATTTGTCACGCACACGGAGTTCCATTGTTGATTAACGATCGTATTGACGTTGCACTTGCATGCGATGCTGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCAGCCTTCTTGGCCCCAATAAGATCATCGGTGTCTCGTGCAAGACACCGGAGCAAGCAGAACAGGCATGGCTTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACAAACACAAAAGCAAACAATCTGACTGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCTGTGGTTGCAATTGGTGGTATTAATCACAGTAATGCAGCAGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTTAAAGTTACATGCAACTTTAGTGGAGGCTACAACATCAAATGTATGAATAATAATTCGTAATGCTTTGATTTTTGTAGTAAGCTTTTTGAAATAAAAAGAACTGTCACAATGTGACTATAACATTATATTTGTATAAGCACATTTATATCCATGATAATTATAATAAGCATGGTAATGGTATATTCATTTTAATT

mRNA sequence

AAAGTCTTAGGATTTTGCAAACTAGTTTCTACAAGAGAGACACAACATGCAATCAGACTTGAATCATTGGTCATCCTAATCAAAACATACATCCAAGAAATTCTAAGTTATTCAATCCTACATTCTTCTAGGGCAAAAATAAAATTAAAAAAAAAAAAAAATCAAATTTGTTTTTAACCACTCTTCTAGCTGTCCTTTTTCAAAACCCTATTTCACGTTCAGCCGCCACCTACCCACCAGTCATTTCACTCCGGCCCGAACCGCCCGCCGTTCCTGCCCGCCACCGACTTAACGGTGCCGCCGCATGCTGCACTCTCAGCTGCTCCAGTCTCAGCCTTCCGACGAAATTGCTGCCGCCTCCTACCTCTCACACTCTCTCGCCTCTCCGAGCAATCAGAGTTACATATCTCTGCTTTGTTTCTCAGAAACACAGTGATTCAGCCCCTAACCGGCGCCGTTGGTGAGATGGTGCCGCTGCCCCTTATTTCTCAGATTCCCAAGTTCAGTCAAGTTTCTAGATTTTGTGTGGCCATGAAGAAGCAGGAAGAAACCGTTGTAGCGTCAAGTAATCGCTATGAGATGAGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGCTCCACTGTGATAACTGCTATTACTGCACAGAACACTGTGGGGGTTCAGGTGAAAACAGGAATGCTACCTTCTACTGGCATTGTTCGGGTTCTACATCAGTGCCTGAAGGAGTTTCCCATTCGAGCCTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGAGATGTTCTGGCTGGTCCTACCATTATTTCAGTGTTACAGGAAGAGCTTCTACCAATGGCTGACTTGGTAACTCCAAATTTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACATTAATCCATCAGATGGGATCAAAGAATGTACTTGTCAAAGGTGGGGACCTTCCCGATTCCTTGGATGCTGTCGATATATTCTTTGATGGCAAGGATTTGCATGAGCTACGATCTTCGCGCATAAAGACTCGCAACACTCATGGTACTGGATGCAGCTTAGCATCATGCATAGCAGCTGAACTCGCTAAAGGGTCTTCAATGTTCTCAGCGGTTAAGTACAGCAAGGACATCAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTCAAGAGTCGAGAACAAAGTTCCTATAGACAGGGTTGTTTCAATCCAGCTGACTTATTCTTGTATGCTGTTACAGACTCAGGTATGAATAAGCATTGGGACCGTTCTATCACCGATGCTGTTAAAGCTGCAGTGGAAGGAGGTGCTACTATTGTTCAAATAAGGGAAAAAGATGCTAAAACTTGCGATTTCTTGGAAGTAGCAAAGTCATGTATGAAGATTTGTCACGCACACGGAGTTCCATTGTTGATTAACGATCGTATTGACGTTGCACTTGCATGCGATGCTGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCAGCCTTCTTGGCCCCAATAAGATCATCGGTGTCTCGTGCAAGACACCGGAGCAAGCAGAACAGGCATGGCTTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACAAACACAAAAGCAAACAATCTGACTGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCTGTGGTTGCAATTGGTGGTATTAATCACAGTAATGCAGCAGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTTAAAGTTACATGCAACTTTAGTGGAGGCTACAACATCAAATGTATGAATAATAATTCGTAATGCTTTGATTTTTGTAGTAAGCTTTTTGAAATAAAAAGAACTGTCACAATGTGACTATAACATTATATTTGTATAAGCACATTTATATCCATGATAATTATAATAAGCATGGTAATGGTATATTCATTTTAATT

Coding sequence (CDS)

ATGGTGCCGCTGCCCCTTATTTCTCAGATTCCCAAGTTCAGTCAAGTTTCTAGATTTTGTGTGGCCATGAAGAAGCAGGAAGAAACCGTTGTAGCGTCAAGTAATCGCTATGAGATGAGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGCTCCACTGTGATAACTGCTATTACTGCACAGAACACTGTGGGGGTTCAGGTGAAAACAGGAATGCTACCTTCTACTGGCATTGTTCGGGTTCTACATCAGTGCCTGAAGGAGTTTCCCATTCGAGCCTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGAGATGTTCTGGCTGGTCCTACCATTATTTCAGTGTTACAGGAAGAGCTTCTACCAATGGCTGACTTGGTAACTCCAAATTTGAAGGAAGCATCTGCCTTACTTGGTGGTATGCCACTTAAAACAATTTCTGACATGCGTCATGCTGCAACATTAATCCATCAGATGGGATCAAAGAATGTACTTGTCAAAGGTGGGGACCTTCCCGATTCCTTGGATGCTGTCGATATATTCTTTGATGGCAAGGATTTGCATGAGCTACGATCTTCGCGCATAAAGACTCGCAACACTCATGGTACTGGATGCAGCTTAGCATCATGCATAGCAGCTGAACTCGCTAAAGGGTCTTCAATGTTCTCAGCGGTTAAGTACAGCAAGGACATCAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTCAAGAGTCGAGAACAAAGTTCCTATAGACAGGGTTGTTTCAATCCAGCTGACTTATTCTTGTATGCTGTTACAGACTCAGGTATGAATAAGCATTGGGACCGTTCTATCACCGATGCTGTTAAAGCTGCAGTGGAAGGAGGTGCTACTATTGTTCAAATAAGGGAAAAAGATGCTAAAACTTGCGATTTCTTGGAAGTAGCAAAGTCATGTATGAAGATTTGTCACGCACACGGAGTTCCATTGTTGATTAACGATCGTATTGACGTTGCACTTGCATGCGATGCTGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCAGCCTTCTTGGCCCCAATAAGATCATCGGTGTCTCGTGCAAGACACCGGAGCAAGCAGAACAGGCATGGCTTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACAAACACAAAAGCAAACAATCTGACTGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCTGTGGTTGCAATTGGTGGTATTAATCACAGTAATGCAGCAGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTTAAAGTTACATGCAACTTTAGTGGAGGCTACAACATCAAATGTATGA

Protein sequence

MVPLPLISQIPKFSQVSRFCVAMKKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQVKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAVKYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEEALKLHATLVEATTSNV

Homology

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BLAST of Lsi11G000210 vs. Swiss-Prot
Match: TPS1L_ARATH (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana GN=TH1 PE=1 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 5.3e-185
Identity = 338/491 (68.84%), Postives = 390/491 (79.43%), Query Frame = 1


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ----------- 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITA+TAQNT GVQ           
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 --------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPTI 159
                         VKTGMLPST IV VL Q L +FP+RALVVDPVMVSTSG VLAG +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLP 219
           +S+ +E LLP+AD++TPN+KEASALL G  ++T+++MR AA  +H+MG + VLVKGGDLP
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLP 208

Query: 220 DSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAVK------ 279
           DS D+VD++FDGK+ HELRS RI TRNTHGTGC+LASCIAAELAKGSSM SAVK      
Sbjct: 209 DSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFV 268

Query: 280 -----YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWDR 339
                YSKDI IG+G QGPFDH   LK   QSS R   FNP DLFLYAVTDS MNK W+R
Sbjct: 269 DNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSS-RCSIFNPDDLFLYAVTDSRMNKKWNR 328

Query: 340 SITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALACD 399
           SI DA+KAA+EGGATI+Q+REK+A+T +FLE AK+C+ IC +HGV LLINDRID+ALACD
Sbjct: 329 SIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACD 388

Query: 400 ADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKAN 459
           ADGVHVGQSD+P   VRSLLGP+KIIGVSCKTPEQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 389 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKAN 448

Query: 460 NLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEE 495
           N T+G+DGLK VC ASKLPVVAIGGI  SNA +VM+I  PNLKGVAVVSALFD+ CVL +
Sbjct: 449 NRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQ 508

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BLAST of Lsi11G000210 vs. Swiss-Prot
Match: TPS1_BRANA (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus GN=BTH1 PE=1 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 2.8e-178
Identity = 326/491 (66.40%), Postives = 383/491 (78.00%), Query Frame = 1


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ----------- 99
           ++  VL+VAGSDSGAGAGIQAD+K CAARGVYC++V TA+ A+NT  VQ           
Sbjct: 31  KVAQVLTVAGSDSGAGAGIQADIKVCAARGVYCASVKTAVKAKNTRAVQSVHLLPPDSVS 90

Query: 100 --------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPTI 159
                         VKTGMLPS  IV VL Q L E+P+RALVVDPVMVSTSG VLAG +I
Sbjct: 91  EQLKSVLSDFEVDVVKTGMLPSPEIVEVLLQNLSEYPVRALVVDPVMVSTSGHVLAGSSI 150

Query: 160 ISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLP 219
           +S+ +E LLP+AD++TPN+KEASALLGG+ ++T+++MR AA  +HQMG + VLVKGGDLP
Sbjct: 151 LSIFRERLLPLADIITPNVKEASALLGGVRIQTVAEMRSAAKSLHQMGPRFVLVKGGDLP 210

Query: 220 DSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAVK------ 279
           DS D+VD++FDG + HEL S RI TRNTHGTGC+LASCIAAELAKGS+M SAVK      
Sbjct: 211 DSSDSVDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNMLSAVKVAKRFV 270

Query: 280 -----YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWDR 339
                YSKDI IG+G QGPFDH   LK  +  SYRQ  F P DLFLYAVTDS MNK W+R
Sbjct: 271 DSALNYSKDIVIGSGMQGPFDHFLSLK--DPQSYRQSTFKPDDLFLYAVTDSRMNKKWNR 330

Query: 340 SITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALACD 399
           SI DAVKAA+EGGATI+Q+REK+A+T +FLE AKSC+ IC ++GV LLINDR D+A+A D
Sbjct: 331 SIVDAVKAAIEGGATIIQLREKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALD 390

Query: 400 ADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKAN 459
           ADGVHVGQSD+P   VRSLLGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 391 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKAN 450

Query: 460 NLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEE 495
           N T+G+DGL+ VC ASKLPVVAIGGI  SNA +VM IG PNLKGVAVVSALFD++CVL +
Sbjct: 451 NRTIGLDGLREVCKASKLPVVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQ 510

[Close]

BLAST of Lsi11G000210 vs. Swiss-Prot
Match: TPS1_ORYSJ (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 622.1 bits (1603), Expect = 5.3e-177
Identity = 319/508 (62.80%), Postives = 387/508 (76.18%), Query Frame = 1


Query: 24  KKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQN 83
           ++      ++S   EM  PHVL+VAGSDSG GAGIQAD+K CAA G YCS+V+TA+TAQN
Sbjct: 39  RRYNRLAASASAAREMPWPHVLTVAGSDSGGGAGIQADIKACAALGAYCSSVVTAVTAQN 98

Query: 84  TVGVQ-------------------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVD 143
           T GVQ                         VKTGMLPS G+VRVL + LK+FP++ALVVD
Sbjct: 99  TAGVQGIHVVPEEFIREQLNSVLSDMSVDVVKTGMLPSIGVVRVLCESLKKFPVKALVVD 158

Query: 144 PVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLI 203
           PVMVSTSGD L+  + +SV ++EL  MAD+VTPN+KEAS LLGG+ L+T+SDMR+AA  I
Sbjct: 159 PVMVSTSGDTLSESSTLSVYRDELFAMADIVTPNVKEASRLLGGVSLRTVSDMRNAAESI 218

Query: 204 HQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELA 263
           ++ G K+VLVKGGD+ +S DA D+FFDGK+  EL + RIKT NTHGTGC+LASCIA+ELA
Sbjct: 219 YKFGPKHVLVKGGDMLESSDATDVFFDGKEFIELHAHRIKTHNTHGTGCTLASCIASELA 278

Query: 264 KGSSMFSAVK-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADL 323
           KG++M  AV+           +SKD+ +GNGPQGPFDHL +LK    +   Q  F P  L
Sbjct: 279 KGATMLHAVQVAKNFVESALHHSKDLVVGNGPQGPFDHLFKLKCPPYNVGSQPSFKPDQL 338

Query: 324 FLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHG 383
           FLYAVTDSGMNK W RSI +AV+AA+EGGATIVQ+REKD++T +FLE AK+CM+IC + G
Sbjct: 339 FLYAVTDSGMNKKWGRSIKEAVQAAIEGGATIVQLREKDSETREFLEAAKACMEICKSSG 398

Query: 384 VPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGA 443
           VPLLINDR+D+ALAC+ADGVHVGQ D+ AHEVR LLGP KIIGVSCKTP QA+QAW DGA
Sbjct: 399 VPLLINDRVDIALACNADGVHVGQLDMSAHEVRELLGPGKIIGVSCKTPAQAQQAWNDGA 458

Query: 444 DYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKG 496
           DYIGCGGV+PT+TKANN T+G DGLK VCLASKLPVVAIGGIN SNA +VME+G+PNLKG
Sbjct: 459 DYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLPVVAIGGINASNAGSVMELGLPNLKG 518

[Close]

BLAST of Lsi11G000210 vs. Swiss-Prot
Match: THIE_ALKOO (Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) GN=thiE PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.1e-44
Identity = 95/206 (46.12%), Postives = 137/206 (66.50%), Query Frame = 1


Query: 286 DLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHA 345
           D  LY V+D  + K   R    +++ A+ GGATIVQ+REK+A + +F ++A     +   
Sbjct: 8   DYGLYLVSDRDVLK--GRDFIKSLEEAILGGATIVQLREKEASSLEFYQLALKAKALTEK 67

Query: 346 HGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLD 405
           + VPL+INDR+D+ALA DADGVHVGQSD+PAH VRS++G NKI+GVS  T E++++A  D
Sbjct: 68  YNVPLIINDRVDIALAVDADGVHVGQSDLPAHIVRSMIGQNKILGVSTATLEESKKAAED 127

Query: 406 GADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNL 465
           GADYIG G ++PT TK +   V +D L+ +     +PVV IGGI   N   +ME+G+   
Sbjct: 128 GADYIGVGALFPTGTKTDANPVTLDQLRYIKENMDIPVVGIGGICEDNIKTIMEVGI--- 187

Query: 466 KGVAVVSALFDRQCVLEEALKLHATL 492
            GVA+VSA+  ++ + E A  L A++
Sbjct: 188 DGVAIVSAILGKENIKEAAESLKASI 208

[Close]

BLAST of Lsi11G000210 vs. Swiss-Prot
Match: THIE_CLOAB (Thiamine-phosphate synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) GN=thiE PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.9e-41
Identity = 90/199 (45.23%), Postives = 131/199 (65.83%), Query Frame = 1


Query: 286 DLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHA 345
           D  LY VTD  + K  +R +  +++ A++GG T+VQ+REK+  T DF E A    KI   
Sbjct: 5   DYKLYLVTDRKVLK--ERDLYKSIEEAIKGGVTLVQLREKEMSTLDFYESALKLKKITET 64

Query: 346 HGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLD 405
           + +PL+INDRID+ALA +ADGVH+GQSD+P  + R LLG +KIIGVS  + E+A +A  +
Sbjct: 65  YKIPLIINDRIDIALAINADGVHIGQSDMPLIKARELLGKDKIIGVSAHSIEEALEAERN 124

Query: 406 GADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNL 465
           GA Y+G G +Y T+TK +   V ++ LK +  + K+PVV IGGIN  NA  V+E GV   
Sbjct: 125 GATYLGVGAIYNTSTKGDAQAVSLEELKNIKNSVKIPVVGIGGINEENANKVIETGV--- 184

Query: 466 KGVAVVSALFDRQCVLEEA 485
            G++V+S +   Q + ++A
Sbjct: 185 DGISVISGILSAQKIKDKA 198

[Close]

BLAST of Lsi11G000210 vs. TrEMBL
Match: D7TGZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 4.1e-208
Identity = 375/492 (76.22%), Postives = 416/492 (84.55%), Query Frame = 1


Query: 39  MRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ---------- 98
           M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA+TAQNTVGVQ          
Sbjct: 1   MKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVNIVPEDFV 60

Query: 99  ---------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPT 158
                          VKTGMLP+ GIV+VLH  LKEFP++ALVVDPVMVSTSGDVLAGP+
Sbjct: 61  AEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALVVDPVMVSTSGDVLAGPS 120

Query: 159 IISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDL 218
           I++  +EELLPMAD+VTPNLKEASALLGG+ L+T+SDM  AA LIH MG +NVLVKGGDL
Sbjct: 121 ILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCTAAKLIHDMGPRNVLVKGGDL 180

Query: 219 PDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAVK----- 278
           P SLDAVDIFFDG D +ELRSSRIKTRNTHGTGC+LASCIAAELAKGS + SAVK     
Sbjct: 181 PSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKGSQILSAVKAAKHY 240

Query: 279 ------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWD 338
                 YSKDI+IGNG QGPFDHL +LKS  ++S+R+  FNPA+LFLYAVTDSGMNK W 
Sbjct: 241 IETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFLYAVTDSGMNKKWG 300

Query: 339 RSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALAC 398
           RSIT+AVKAA+EGGATIVQ+REKDA+T DFLE AK+C++ICH+HGVPLLINDRIDVALAC
Sbjct: 301 RSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVPLLINDRIDVALAC 360

Query: 399 DADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKA 458
           DADGVHVGQSDIPA  VR+LLGP KIIGVSCKTPEQAE+AW+DGADYIGCGGVYPTNTKA
Sbjct: 361 DADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADYIGCGGVYPTNTKA 420

Query: 459 NNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLE 495
           NN+TVG+DGLK VCLASKLPVVAIGGIN SNA  VMEIGVPNLKGVAVVSALFDR+CVL 
Sbjct: 421 NNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVAVVSALFDRECVLT 480

[Close]

BLAST of Lsi11G000210 vs. TrEMBL
Match: M5XRD0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003609mg PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 1.9e-205
Identity = 370/513 (72.12%), Postives = 420/513 (81.87%), Query Frame = 1


Query: 21  VAMKKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 80
           + M+ Q      +SN+  + IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA+T
Sbjct: 48  IFMEMQGNGSPTTSNQSTINIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVT 107

Query: 81  AQNTVGVQ-------------------------VKTGMLPSTGIVRVLHQCLKEFPIRAL 140
           AQNT GVQ                         VKTGMLPS GIV++LHQ L+E+P+RAL
Sbjct: 108 AQNTAGVQGVNVVPEDFVAEQMKSVLSDMHVDVVKTGMLPSIGIVKILHQHLREYPVRAL 167

Query: 141 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 200
           VVDPVMVSTSGDVLAGP++++  +EELLPMA+++TPNLKEASALL G+ +KT+SDMR AA
Sbjct: 168 VVDPVMVSTSGDVLAGPSVLAGFREELLPMANIITPNLKEASALLDGVKIKTVSDMRSAA 227

Query: 201 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAA 260
            L+H  G++NVLVKGGDLPDSLDAVDIFFDG+ L+ELRSSRIKTRNTHGTGC+LASCIAA
Sbjct: 228 KLLHDKGARNVLVKGGDLPDSLDAVDIFFDGEHLYELRSSRIKTRNTHGTGCTLASCIAA 287

Query: 261 ELAKGSSMFSAVK-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNP 320
           ELAKG+SM  AVK           YSK+I IGNGPQGPFDHL +LKS   +S RQ  FNP
Sbjct: 288 ELAKGASMLEAVKVAKCFVETALDYSKEIFIGNGPQGPFDHLMKLKSNAHNSGRQVRFNP 347

Query: 321 ADLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICH 380
           +DLFLYAVTDSGMN+ W  SI+DAVKAAV+GGATIVQ+REKD +T DF+E AKSC++IC 
Sbjct: 348 SDLFLYAVTDSGMNRRWGHSISDAVKAAVQGGATIVQLREKDIETRDFVEAAKSCLQICR 407

Query: 381 AHGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWL 440
           AHGVPLLINDRIDVALACDADGVH+GQSD+PAH  R+LLGP KIIGVSCKTPEQAEQAW+
Sbjct: 408 AHGVPLLINDRIDVALACDADGVHIGQSDMPAHTARALLGPEKIIGVSCKTPEQAEQAWI 467

Query: 441 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN 498
            GADYIGCGGVYPTNTKANNLTVG+DGLK VCLASKLPVVAIGGI  SNA  VMEIGVPN
Sbjct: 468 AGADYIGCGGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIKVSNARPVMEIGVPN 527

[Close]

BLAST of Lsi11G000210 vs. TrEMBL
Match: A0A067EGP5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010260mg PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 8.2e-201
Identity = 361/498 (72.49%), Postives = 411/498 (82.53%), Query Frame = 1


Query: 33  SSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ---- 92
           ++ +Y+M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA+TAQNT GVQ    
Sbjct: 12  TTEQYKMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTAGVQGVNI 71

Query: 93  ---------------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGD 152
                                VKTGMLPST +V+VL Q L EFP+RALVVDPVMVSTSGD
Sbjct: 72  VPEDFVAAQLKSVLSDMQVDVVKTGMLPSTDLVKVLLQSLSEFPVRALVVDPVMVSTSGD 131

Query: 153 VLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVL 212
           VLAGP+ I+ L+E LLPMAD+VTPN+KEASALLGGM + T++DM  AA L+H +G + VL
Sbjct: 132 VLAGPSTITGLRENLLPMADIVTPNVKEASALLGGMQVVTVADMCSAAKLLHNLGPRTVL 191

Query: 213 VKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAV 272
           VKGGDLPDS DAVDIFFDG+D HELRSSR+ TRNTHGTGC+LASCIAAELAKGS M SAV
Sbjct: 192 VKGGDLPDSSDAVDIFFDGEDFHELRSSRVNTRNTHGTGCTLASCIAAELAKGSPMLSAV 251

Query: 273 K-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSG 332
           K           YSKDI IG+GPQGPFDHL RLKS  + S+R   FNP+DLFLYAVTDSG
Sbjct: 252 KVAKCFVETALDYSKDIVIGSGPQGPFDHLLRLKSTSRQSHRAEAFNPSDLFLYAVTDSG 311

Query: 333 MNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRI 392
           MNK W RSITDAVKAA+EGGATI+Q+REKDA T  FLE AK+C++IC  HGVPLLINDRI
Sbjct: 312 MNKKWGRSITDAVKAALEGGATIIQLREKDADTRGFLEAAKACLQICCVHGVPLLINDRI 371

Query: 393 DVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVY 452
           D+ALACDADGVH+GQSD+PA   R+LLGP+KIIGVSCKTPE+A QAW+DGA+YIGCGGVY
Sbjct: 372 DIALACDADGVHLGQSDMPARTARALLGPDKIIGVSCKTPEEAHQAWIDGANYIGCGGVY 431

Query: 453 PTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFD 495
           PTNTKANNLTVG+DGLK VCLASKLPVVAIGGI  SNA+ VM+IGV NLKGVAVVSALFD
Sbjct: 432 PTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIGISNASDVMKIGVSNLKGVAVVSALFD 491

[Close]

BLAST of Lsi11G000210 vs. TrEMBL
Match: V4T108_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 1.6e-199
Identity = 359/498 (72.09%), Postives = 408/498 (81.93%), Query Frame = 1


Query: 33  SSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ---- 92
           ++ +Y+M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA+TAQNT GVQ    
Sbjct: 12  TTEQYKMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTAGVQGVNI 71

Query: 93  ---------------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGD 152
                                VKTGMLPST + +VL Q L EFP+RALVVDPVMVSTSGD
Sbjct: 72  VPEDFVAAQLKSVLSDMQVDVVKTGMLPSTDLAKVLLQSLSEFPVRALVVDPVMVSTSGD 131

Query: 153 VLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVL 212
           VLAGP+ I+ L+E LLPMAD+VTPN+KEASALLGGM + T++DM  AA L+H +G + VL
Sbjct: 132 VLAGPSTITGLRENLLPMADIVTPNVKEASALLGGMQVVTVADMCSAAKLLHNLGPRTVL 191

Query: 213 VKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAV 272
           VKGGDLPDS DAVDIFFDG+D HELRSSR+ TRNTHGTGC+LASCIAAELAKGS M SAV
Sbjct: 192 VKGGDLPDSSDAVDIFFDGEDFHELRSSRVNTRNTHGTGCTLASCIAAELAKGSPMLSAV 251

Query: 273 K-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSG 332
           K           YSKDI IG+GPQGPFDHL RLKS    S+R   FNP+DLFLYAVTDSG
Sbjct: 252 KVAKCFVETALDYSKDIVIGSGPQGPFDHLLRLKSTSHQSHRAEAFNPSDLFLYAVTDSG 311

Query: 333 MNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRI 392
            NK W RSITDAVKAA+EGGATI+Q+REKDA T  FLE AK+C++IC  HGVPLLINDRI
Sbjct: 312 TNKKWGRSITDAVKAALEGGATIIQLREKDADTRGFLEAAKACLQICCVHGVPLLINDRI 371

Query: 393 DVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVY 452
           D+ALACDADGVH+GQSD+PA   R+LLGP+KIIGVSCKTPE+A QAW+DGA+YIGCGGVY
Sbjct: 372 DIALACDADGVHLGQSDMPARTARALLGPDKIIGVSCKTPEEAHQAWIDGANYIGCGGVY 431

Query: 453 PTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFD 495
           PTNTKANNLTVG+DGLK VCLASKLPVVAIGGI  SNA+ VM+IGV NLKGVAVVSALFD
Sbjct: 432 PTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIGISNASDVMKIGVSNLKGVAVVSALFD 491

[Close]

BLAST of Lsi11G000210 vs. TrEMBL
Match: V4T678_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 1.6e-199
Identity = 359/498 (72.09%), Postives = 408/498 (81.93%), Query Frame = 1


Query: 33  SSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ---- 92
           ++ +Y+M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA+TAQNT GVQ    
Sbjct: 40  TTEQYKMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTAGVQGVNI 99

Query: 93  ---------------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGD 152
                                VKTGMLPST + +VL Q L EFP+RALVVDPVMVSTSGD
Sbjct: 100 VPEDFVAAQLKSVLSDMQVDVVKTGMLPSTDLAKVLLQSLSEFPVRALVVDPVMVSTSGD 159

Query: 153 VLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVL 212
           VLAGP+ I+ L+E LLPMAD+VTPN+KEASALLGGM + T++DM  AA L+H +G + VL
Sbjct: 160 VLAGPSTITGLRENLLPMADIVTPNVKEASALLGGMQVVTVADMCSAAKLLHNLGPRTVL 219

Query: 213 VKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAV 272
           VKGGDLPDS DAVDIFFDG+D HELRSSR+ TRNTHGTGC+LASCIAAELAKGS M SAV
Sbjct: 220 VKGGDLPDSSDAVDIFFDGEDFHELRSSRVNTRNTHGTGCTLASCIAAELAKGSPMLSAV 279

Query: 273 K-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSG 332
           K           YSKDI IG+GPQGPFDHL RLKS    S+R   FNP+DLFLYAVTDSG
Sbjct: 280 KVAKCFVETALDYSKDIVIGSGPQGPFDHLLRLKSTSHQSHRAEAFNPSDLFLYAVTDSG 339

Query: 333 MNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRI 392
            NK W RSITDAVKAA+EGGATI+Q+REKDA T  FLE AK+C++IC  HGVPLLINDRI
Sbjct: 340 TNKKWGRSITDAVKAALEGGATIIQLREKDADTRGFLEAAKACLQICCVHGVPLLINDRI 399

Query: 393 DVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVY 452
           D+ALACDADGVH+GQSD+PA   R+LLGP+KIIGVSCKTPE+A QAW+DGA+YIGCGGVY
Sbjct: 400 DIALACDADGVHLGQSDMPARTARALLGPDKIIGVSCKTPEEAHQAWIDGANYIGCGGVY 459

Query: 453 PTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFD 495
           PTNTKANNLTVG+DGLK VCLASKLPVVAIGGI  SNA+ VM+IGV NLKGVAVVSALFD
Sbjct: 460 PTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIGISNASDVMKIGVSNLKGVAVVSALFD 519

[Close]

BLAST of Lsi11G000210 vs. TAIR10
Match: AT1G22940.1 (AT1G22940.1 thiamin biosynthesis protein, putative)

HSP 1 Score: 648.7 bits (1672), Expect = 3.0e-186
Identity = 338/491 (68.84%), Postives = 390/491 (79.43%), Query Frame = 1


Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTVGVQ----------- 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITA+TAQNT GVQ           
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 --------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPTI 159
                         VKTGMLPST IV VL Q L +FP+RALVVDPVMVSTSG VLAG +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLP 219
           +S+ +E LLP+AD++TPN+KEASALL G  ++T+++MR AA  +H+MG + VLVKGGDLP
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALLDGFRIETVAEMRSAAKSLHEMGPRFVLVKGGDLP 208

Query: 220 DSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKGSSMFSAVK------ 279
           DS D+VD++FDGK+ HELRS RI TRNTHGTGC+LASCIAAELAKGSSM SAVK      
Sbjct: 209 DSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSMLSAVKVAKRFV 268

Query: 280 -----YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWDR 339
                YSKDI IG+G QGPFDH   LK   QSS R   FNP DLFLYAVTDS MNK W+R
Sbjct: 269 DNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSS-RCSIFNPDDLFLYAVTDSRMNKKWNR 328

Query: 340 SITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALACD 399
           SI DA+KAA+EGGATI+Q+REK+A+T +FLE AK+C+ IC +HGV LLINDRID+ALACD
Sbjct: 329 SIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACD 388

Query: 400 ADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKAN 459
           ADGVHVGQSD+P   VRSLLGP+KIIGVSCKTPEQA QAW DGADYIG GGV+PTNTKAN
Sbjct: 389 ADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKAN 448

Query: 460 NLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEE 495
           N T+G+DGLK VC ASKLPVVAIGGI  SNA +VM+I  PNLKGVAVVSALFD+ CVL +
Sbjct: 449 NRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQ 508

[Close]

BLAST of Lsi11G000210 vs. NCBI nr
Match: gi|659120551|ref|XP_008460243.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 913.7 bits (2360), Expect = 1.4e-262
Identity = 471/535 (88.04%), Postives = 488/535 (91.21%), Query Frame = 1


Query: 1   MVPLPLISQIPKFSQVSRFCVAMKKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPKF+QVSRFC+AMKKQEE VVASS+RYE RIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQ-------------------------VKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQ                         VKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIV+VLHQCLKEFP+RALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKTRNTHGTGCSLASCIAAELAKGSSMFSAVK-----------YSKDISIGNGPQGPFD 300
           RIK+RNTHGTGCSLASCI+AELAKGSSMFSAVK           YSKDI+IG+GPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCISAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIRE 360
           HLCRLKSREQSSY QGCFNP DLFLYAVTDSGMN+ WDRSITDAVKAAVEGGATIVQIRE
Sbjct: 301 HLCRLKSREQSSYSQGCFNPTDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIRE 360

Query: 361 KDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLG 420
           KDAKT DFLEVAKSC+KICHAHGVPLLINDRID+ALACDADGVHVGQSDIPAHEVR LLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNK+IGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV
Sbjct: 421 PNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEEALKLHATLVEATTSNV 500
           AIGGINH+NAAAVM IG+PNL+GVAVVSALFDRQCVLE A KLHATLVEATTSNV
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATTSNV 535

[Close]

BLAST of Lsi11G000210 vs. NCBI nr
Match: gi|449445302|ref|XP_004140412.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis sativus])

HSP 1 Score: 882.1 bits (2278), Expect = 4.6e-253
Identity = 458/532 (86.09%), Postives = 477/532 (89.66%), Query Frame = 1


Query: 1   MVPLPLISQIPKFSQVSRFCVAMKKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQA 60
           MVPLPLISQIPKF+QVSRFC+AMKKQ+E VVASSNR E  IPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQDEMVVASSNRNETCIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAITAQNTVGVQ-------------------------VKTGMLP 120
           DLKTCAARGVYCSTVITAITAQNTVGVQ                         VKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNVVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIVRVLHQCLKEFPIRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180
           STGIV+VLHQCLKEFP+RALVVDPVMVSTSGDVLAGP IISVLQE+LLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPAIISVLQEKLLPMADLVTPNLKE 180

Query: 181 ASALLGGMPLKTISDMRHAATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSS 240
           ASALLG MPL TISDMRHAA LI+QMGSKNVL+KGGDLPDSLDAVDIFFDGKDLHELRSS
Sbjct: 181 ASALLGDMPLTTISDMRHAAMLIYQMGSKNVLIKGGDLPDSLDAVDIFFDGKDLHELRSS 240

Query: 241 RIKTRNTHGTGCSLASCIAAELAKGSSMFSAVK-----------YSKDISIGNGPQGPFD 300
           RIK+RNTHGTGCSLASCIAAELAKGSSMFSAVK           YSKDI+IG+GPQGPFD
Sbjct: 241 RIKSRNTHGTGCSLASCIAAELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFD 300

Query: 301 HLCRLKSREQSSYRQGCFNPADLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIRE 360
           HLC LK+RE SSY QGCFNPADLFLYAVTDSGMN+ WDRSITDAVK AVEGGATIVQIRE
Sbjct: 301 HLCCLKNREPSSYSQGCFNPADLFLYAVTDSGMNERWDRSITDAVKDAVEGGATIVQIRE 360

Query: 361 KDAKTCDFLEVAKSCMKICHAHGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLG 420
           KDAKT DFLEVAKSC+KIC AHGVPLLINDRID+ALAC+ADGVHVGQSDIPAHEVR LLG
Sbjct: 361 KDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRLLG 420

Query: 421 PNKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480
           PNKIIGVSCKT EQAEQAW+DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV
Sbjct: 421 PNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVV 480

Query: 481 AIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEEALKLHATLVEATT 497
           AIGGINH+NAAAVM IG+PNLKGVAVVSALFDRQCVLEEA KLHATLVEATT
Sbjct: 481 AIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 532

[Close]

BLAST of Lsi11G000210 vs. NCBI nr
Match: gi|659120553|ref|XP_008460244.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 876.3 bits (2263), Expect = 2.5e-251
Identity = 452/515 (87.77%), Postives = 468/515 (90.87%), Query Frame = 1


Query: 21  VAMKKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 80
           +AMKKQEE VVASS+RYE RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT
Sbjct: 1   MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 60

Query: 81  AQNTVGVQ-------------------------VKTGMLPSTGIVRVLHQCLKEFPIRAL 140
           AQNTVGVQ                         VKTGMLPSTGIV+VLHQCLKEFP+RAL
Sbjct: 61  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 120

Query: 141 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 200
           VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA
Sbjct: 121 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAA 180

Query: 201 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAA 260
           TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIK+RNTHGTGCSLASCI+A
Sbjct: 181 TLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISA 240

Query: 261 ELAKGSSMFSAVK-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNP 320
           ELAKGSSMFSAVK           YSKDI+IG+GPQGPFDHLCRLKSREQSSY QGCFNP
Sbjct: 241 ELAKGSSMFSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNP 300

Query: 321 ADLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICH 380
            DLFLYAVTDSGMN+ WDRSITDAVKAAVEGGATIVQIREKDAKT DFLEVAKSC+KICH
Sbjct: 301 TDLFLYAVTDSGMNERWDRSITDAVKAAVEGGATIVQIREKDAKTRDFLEVAKSCIKICH 360

Query: 381 AHGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWL 440
           AHGVPLLINDRID+ALACDADGVHVGQSDIPAHEVR LLGPNK+IGVSCKT EQAEQAW+
Sbjct: 361 AHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWI 420

Query: 441 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPN 500
           DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINH+NAAAVM IG+PN
Sbjct: 421 DGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPN 480

[Close]

BLAST of Lsi11G000210 vs. NCBI nr
Match: gi|731411629|ref|XP_010658056.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Vitis vinifera])

HSP 1 Score: 733.4 bits (1892), Expect = 2.6e-208
Identity = 380/512 (74.22%), Postives = 424/512 (82.81%), Query Frame = 1


Query: 19  FCVAMKKQEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITA 78
           F V  + +  TV   S   +M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA
Sbjct: 52  FTVMQEDRPSTVKDDS---KMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITA 111

Query: 79  ITAQNTVGVQ-------------------------VKTGMLPSTGIVRVLHQCLKEFPIR 138
           +TAQNTVGVQ                         VKTGMLP+ GIV+VLH  LKEFP++
Sbjct: 112 VTAQNTVGVQGVNIVPEDFVAEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQ 171

Query: 139 ALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRH 198
           ALVVDPVMVSTSGDVLAGP+I++  +EELLPMAD+VTPNLKEASALLGG+ L+T+SDM  
Sbjct: 172 ALVVDPVMVSTSGDVLAGPSILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCT 231

Query: 199 AATLIHQMGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCI 258
           AA LIH MG +NVLVKGGDLP SLDAVDIFFDG D +ELRSSRIKTRNTHGTGC+LASCI
Sbjct: 232 AAKLIHDMGPRNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCI 291

Query: 259 AAELAKGSSMFSAVK-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCF 318
           AAELAKGS + SAVK           YSKDI+IGNG QGPFDHL +LKS  ++S+R+  F
Sbjct: 292 AAELAKGSQILSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAF 351

Query: 319 NPADLFLYAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKI 378
           NPA+LFLYAVTDSGMNK W RSIT+AVKAA+EGGATIVQ+REKDA+T DFLE AK+C++I
Sbjct: 352 NPANLFLYAVTDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEI 411

Query: 379 CHAHGVPLLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQA 438
           CH+HGVPLLINDRIDVALACDADGVHVGQSDIPA  VR+LLGP KIIGVSCKTPEQAE+A
Sbjct: 412 CHSHGVPLLINDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKA 471

Query: 439 WLDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGV 495
           W+DGADYIGCGGVYPTNTKANN+TVG+DGLK VCLASKLPVVAIGGIN SNA  VMEIGV
Sbjct: 472 WIDGADYIGCGGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGV 531

[Close]

BLAST of Lsi11G000210 vs. NCBI nr
Match: gi|731411633|ref|XP_010658058.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Vitis vinifera])

HSP 1 Score: 733.4 bits (1892), Expect = 2.6e-208
Identity = 377/505 (74.65%), Postives = 421/505 (83.37%), Query Frame = 1


Query: 26  QEETVVASSNRYEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAITAQNTV 85
           QE+      +  +M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITA+TAQNTV
Sbjct: 2   QEDRPSTVKDDSKMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTV 61

Query: 86  GVQ-------------------------VKTGMLPSTGIVRVLHQCLKEFPIRALVVDPV 145
           GVQ                         VKTGMLP+ GIV+VLH  LKEFP++ALVVDPV
Sbjct: 62  GVQGVNIVPEDFVAEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALVVDPV 121

Query: 146 MVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLGGMPLKTISDMRHAATLIHQ 205
           MVSTSGDVLAGP+I++  +EELLPMAD+VTPNLKEASALLGG+ L+T+SDM  AA LIH 
Sbjct: 122 MVSTSGDVLAGPSILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCTAAKLIHD 181

Query: 206 MGSKNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKTRNTHGTGCSLASCIAAELAKG 265
           MG +NVLVKGGDLP SLDAVDIFFDG D +ELRSSRIKTRNTHGTGC+LASCIAAELAKG
Sbjct: 182 MGPRNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKG 241

Query: 266 SSMFSAVK-----------YSKDISIGNGPQGPFDHLCRLKSREQSSYRQGCFNPADLFL 325
           S + SAVK           YSKDI+IGNG QGPFDHL +LKS  ++S+R+  FNPA+LFL
Sbjct: 242 SQILSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFL 301

Query: 326 YAVTDSGMNKHWDRSITDAVKAAVEGGATIVQIREKDAKTCDFLEVAKSCMKICHAHGVP 385
           YAVTDSGMNK W RSIT+AVKAA+EGGATIVQ+REKDA+T DFLE AK+C++ICH+HGVP
Sbjct: 302 YAVTDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVP 361

Query: 386 LLINDRIDVALACDADGVHVGQSDIPAHEVRSLLGPNKIIGVSCKTPEQAEQAWLDGADY 445
           LLINDRIDVALACDADGVHVGQSDIPA  VR+LLGP KIIGVSCKTPEQAE+AW+DGADY
Sbjct: 362 LLINDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADY 421

Query: 446 IGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVA 495
           IGCGGVYPTNTKANN+TVG+DGLK VCLASKLPVVAIGGIN SNA  VMEIGVPNLKGVA
Sbjct: 422 IGCGGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVA 481

The following BLAST results are available for this feature:

BLAST of Lsi11G000210 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TPS1L_ARATH	5.3e-185	68.84	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal...	[more]
TPS1_BRANA	2.8e-178	66.40	Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ...	[more]
TPS1_ORYSJ	5.3e-177	62.80	Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ...	[more]
THIE_ALKOO	4.1e-44	46.12	Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) GN=thiE P...	[more]
THIE_CLOAB	1.9e-41	45.23	Thiamine-phosphate synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM...	[more]

BLAST of Lsi11G000210 vs. TrEMBL


Match Name	E-value	Identity	Description
D7TGZ0_VITVI	4.1e-208	76.22	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=...	[more]
M5XRD0_PRUPE	1.9e-205	72.12	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003609mg PE=3 SV=1	[more]
A0A067EGP5_CITSI	8.2e-201	72.49	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010260mg PE=3 SV=1	[more]
V4T108_9ROSI	1.6e-199	72.09	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1	[more]
V4T678_9ROSI	1.6e-199	72.09	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1	[more]

BLAST of Lsi11G000210 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G22940.1	3.0e-186	68.84	thiamin biosynthesis protein, putative	[more]

BLAST of Lsi11G000210 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659120551\|ref\|XP_008460243.1\|	1.4e-262	88.04	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
gi\|449445302\|ref\|XP_004140412.1\|	4.6e-253	86.09	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis...	[more]
gi\|659120553\|ref\|XP_008460244.1\|	2.5e-251	87.77	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
gi\|731411629\|ref\|XP_010658056.1\|	2.6e-208	74.22	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
gi\|731411633\|ref\|XP_010658058.1\|	2.6e-208	74.65	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity
GO:0004789	thiamine-phosphate diphosphorylase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0009228	thiamine biosynthetic process

Vocabulary: **INTERPRO**
Term	Definition
IPR022998	ThiamineP_synth_TenI
IPR013785	Aldolase_TIM
IPR013749	PM/HMP-P_kinase-1
IPR003733	Thiamine phosphate synthase

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0008972	phosphomethylpyrimidine kinase activity
molecular_function	GO:0004789	thiamine-phosphate diphosphorylase activity
molecular_function	GO:0008902	hydroxymethylpyrimidine kinase activity
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G000210.1	Lsi11G000210.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003733	Thiamine phosphate synthase	HAMAP	MF_00097	TMP_synthase	coord: 286..493 score: 26
IPR003733	Thiamine phosphate synthase	PFAM	PF02581	TMP-TENI	coord: 289..474 score: 1.6
IPR003733	Thiamine phosphate synthase	TIGRFAMs	TIGR00693	TIGR00693	coord: 289..478 score: 6.5
IPR013749	Pyridoxamine kinase/Phosphomethylpyrimidine kinase	PFAM	PF08543	Phos_pyr_kin	coord: 89..252 score: 1.2E-52coord: 51..88 score: 1.0
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 283..491 score: 1.3
IPR022998	Thiamin phosphate synthase superfamily	unknown	SSF51391	Thiamin phosphate synthase	coord: 285..487 score: 4.97
None	No IPR available	PANTHER	PTHR20858	PHOSPHOMETHYLPYRIMIDINE KINASE	coord: 28..305 score: 2.6E
None	No IPR available	PANTHER	PTHR20858:SF17	HYDROXYMETHYLPYRIMIDINE/PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED	coord: 28..305 score: 2.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi11G000210	Csa5G262270	Cucumber (Chinese Long) v2	culsiB336
Lsi11G000210	CSPI05G12990	Wild cucumber (PI 183967)	cpilsiB337
Lsi11G000210	CmoCh04G019850	Cucurbita moschata (Rifu)	cmolsiB621
Lsi11G000210	MELO3C022605	Melon (DHL92) v3.5.1	lsimeB083
Lsi11G000210	MELO3C022605.2	Melon (DHL92) v3.6.1	lsimedB088
Lsi11G000210	Cla97C11G207240	Watermelon (97103) v2	lsiwmbB079
Lsi11G000210	BhiUN306G7	Wax gourd	lsiwgoB099
Lsi11G000210	CsGy5G009580	Cucumber (Gy14) v2	cgyblsiB311
Lsi11G000210	CsaV3_5G012850	Cucumber (Chinese Long) v3	cuclsiB363

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi11G000210	Watermelon (Charleston Gray)	lsiwcgB087
Lsi11G000210	Watermelon (Charleston Gray)	lsiwcgB084
Lsi11G000210	Watermelon (97103) v1	lsiwmB114
Lsi11G000210	Watermelon (97103) v1	lsiwmB109
Lsi11G000210	Cucurbita pepo (Zucchini)	cpelsiB319