CmoCh04G019850 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh04G019850
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionHydroxymethylpyrimidine kinase / phosphomethylpyrimidine kinase / thiamine-phosphate diphosphorylase
LocationCmo_Chr04 : 10279007 .. 10283100 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCATTTTTATTGGTTTAAACCATTATTACTTTCCTTTTACTTAACCCTACCTCACGGTCAGCCAGCCTCCACCGCCCATTACCAGTCACTGTACTCCGTCCCGCCGCCGCCTCCTCCTCCCCCCTCACATTCTCTCGCCTCCTTAAGGACTCAGAGTCACATTTCTTTGTTTAATTCCCTCAGAAACACAGTGGTTCAGCCCCTAACCCGCGCCGTTCTTGAGATGGTGCTGCTGCCTCTCACTTTTCAGATCCCCAAGGTCTGATTTATTTATTTATTTATTTTTGTTCTTTTTCGTTGGGTTATGTTGGTTTATGCGCTTCACCCACGGAGAAGAAAACTAATAATGTATCCTTCGCAAATTAGACAGAAAACACCGATTGAACCAACAAGTACAGAGGAACAAAAAACAAACCAATTCTTGTCTGAATCAGTAAATTTATGGGAAAAGTAATGTTTAAGAATGAACAACGCTTAAAAAGACCCTGTAGTTTTGTTGGCTATGAAAAACTTGTTGGAATTGAAAAGCTGCATTTGAAAACATTGAAACTATAGAAAACTATGGACAAACAAAATAGAAAACTTAACTTTTTGATTTTAGTTTGATTGAATGACGTTGAGTCTTCTTGAAAATAGAAGAAACTATCCACGGAGAGCAGAAATTATTATCTATTTTCTTAAAAAATTTCATATGGTGTAGAATTGGAAACAGAATTTTTAAAGTATTAAATCCTTACAAATCAGACCTATATAGAAGTATATTTTATGATTTATGGGTTTTACTTTCTGTGCCTTGATTTGTCCGTTTTAGTTCAATCAAGTCTCTAGGTTTTGTATGGCCATGAAGAAGCCGGAAGAAACAGTTGTAGCTTCAAGTGATCGCCATGAGATGAGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGTTCCACTGTGATAACTGCTGTTACTGCACAGAACACCGTGGGGGTTCAGGTCATTATCGCGCACACATAATTATTATCCATATTTATTTGTTGGTGTATTTGTTTTGGCTTGAAAGGACAATAGGGAGGACTGGCGAATAAATAAATAATGTTCCTTGCCTGCCTGCTATGGTATAATGAGGTGTATAATGTTATTGTGAGTTTAGGATGATTTTTGAAAACAAGGTACTTCTGAGTGAAAGCTTATCCTTTGCTAGCACTTTATTAAGTGCTATCCTGGGTATGTTTAGGAATGCTTTTGGCTTGGTGAAAAACACCTTTTTCTTATGCTCAAAAGCATGTATCGTAAACATCATGTTTGGGAGCAACAACAGATGCTTTTAATAAAAGTTAATAGTACTTATAATACTTGGTAAAAAGCACTTTTAGATTTCTTTTGTCTAACTGGGAAGTACTTTATAAACACTTATTAATAGTGTGAAAGCAAGCATAAAGTAATCTTTCGTAAAGCAGTTCTTTTCAAAATGCTTACAGTACTTCATCCTTATTCTATAAGCACTCCTAAATACATCCGTAGTCCTTTTAATAAGTTTTCAAGTATCACTTTTAAGTATTTAACTCAAATTATGAAAACTTTGAAAATACTTTTGACAAGCCAGAAACACTTTTTACCTTCGCGAAAATCACCCTAAACTCACTCTTAGAATATACAATGCCCTTGAAGTTAGTTTTTGCTTTGTTCTCAATGAAATTAACTGAAATGTTTTGTCCTAAATTATACTGCTTGTAACACAAATTTTATTTAACACGAAGATGTACTAACTTTTTCAGGATGTAAACATTATACCGGAGGGCTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAGTATAACTAAATTTTAAGTTAGGATTGCCTTGCAAGTTTTTTAGGAGCTCATACGGACAAGACTGTGGACTGTTCATGTATAGGATATTGAACAGGCTAGCTGTTATTGCTTTTTCAGTGCACGTACTAATATATACTTTATGTAAGCTGGAAAACACAGAAAGACTACTAATGATTACATTAATTCAGGTGAAAACAGGAATGCTACCTTCTACTGGCATCATTCAGGTTATACATCAGCGCCTGAAGGAGTTTCCTGTTCAAGGTATAAAGTATTTCAGTAAGTTATTGGTGCATTTCTTTGATTTAATTATCGTTTTTTAAAGATTTTAATATGAATAAAGGCAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGGGACGTTCTGGCTTGTCCTACAATTATTTCAGTGTTACAGTAAGTTGATTATCTGTTTATAGGGAGTATCATTAGTCCTTCAAACTAGTTCTTATGATAATTTCTTGCGCTTCCTGTTTGCATTTTTTAAATGTCCTCAGAATTAGTATATTGTTTTTTGGTTCAAAAGGACTCTGAAGCTTTTGGTATGGACTTCAAGCTTTTAGCGTTAAGGCTTACATCGAACAAACCTTTATTAGAACCTACTGAGGCTTGAAATTCTTATGCGTCATATAATAATTAGAAAAATAATACTATTTTATTGCCTATTCTCTCAAAATGTGACTGTTCACATGTGTAAGTAATGCGTATAGCACCATGATTTATTCATGCTTTTGGCTAATGTCCATACATCCAAGTAAGGACTACATGAAGCTCCCAACAATTTAATACTGTTTTTAATGGTATTGCTACTGGTTTTACTTTCACATGTTAGATTGAGTGATTTTGCAAAACCCCTTCGTTTGCAAGGGGACATCTTGTCCGTTTTTCCATAGATTGTTTTGGAAGCTTCTTTTTTATAACGAAATTTCTTGCATGTTTCTTATAAAAAGAAGAGAGAGAGAGCGTGAAAGGGAGAAAAATCTTCTTGGTAGTAGCTTCTTCTTAAAGTTGCTGGTTTTTCTTGTTGAGAGAGGCTTTTAGTTTTGACTTGGCAAAACTTGTTAGTCTTTGTTTGGCTGCAATTACTCCCACAAATTTGCCTCACATTGGCCCCAAGGTGTGTTCTTGTTGGTAAGAAATTACAATATTGGAACCCCTCCCAAATTTTTAATTCAAACAGTGGGTTCTAGGGATCGAGAGAATTAATTTCCTTGCCCATCTACTAGAGCTGGAGCTGCGGGTTTTGCTGGGGATTAGAGTTGGTGGGGCCTGGATTCCCCAATTTACAGCCATAATTTGCTTAATTGAATGAAAATAATGCTTTTTCTTTAATCAAAATTCATGTCTGATCTTCATATGTTAGTTTTTCATAATTCTATGACACGTCTTTTGCTTTCAGGGATGAACTTCTACCAATGGCTGACTTGGTAACCCCAAATTTGAAGGAAGCATCTGCCTTACTTGGCGGTATGCCCCTTAATACAATTTCTGACATGCGTCATGCTGCAACACTAATCCATCAGATGGGATCCAAGTAAGTTTTTAATCCCTCACATAGCTTGCCTTCTTTTGTAATTCCATTTTATCAATGAAATGAAAATCAATGTTTCTCATGAAAAAAGAAGAAATTTTTATGCTACCTCTAGTCACAAGAGACCGACTTTGGCACTCATTTTTCCTCATTGCCGCCTTCCTCCTAGCAGTAATGTTAATACCATTCTACTTAATTCAAAGAAGTTTTTTTAAGTCTAAATTATAAAAATACCCCTGAATTTTACCCTTTGTTTCAAAAGTATCTCTATGCTTTTAAAATTTGCAATATTACCCTTGTGTTGTGATCTAGAACAATATTTTGGATCATAATATTGGAACTTGTGAAGTATAGGAGTATTTTTGAAACACAGAAAAAAGTTCAGAGGTGTCTTTTATAATTTAACCATTTTTAAAACTTTAATTCTATGGTTGTTCAGATTACATTAGCGATCATTAGTCATTCTCCCTCCTTTTCCACCCAATCTTCACCTTCTTATAGACCCATCGAATCTTCTCCCCCCTGCCTCCTCAACAATCGCTTGCCATCCTGACCCCGATCCCTTCTTCCTTTCCCTCCTCATCAGCCAATTAATCTACCTCATTGCATTTACCAATTGGCCTCAAGACGACTAA

mRNA sequence

TCATTTTTATTGGTTTAAACCATTATTACTTTCCTTTTACTTAACCCTACCTCACGGTCAGCCAGCCTCCACCGCCCATTACCAGTCACTGTACTCCGTCCCGCCGCCGCCTCCTCCTCCCCCCTCACATTCTCTCGCCTCCTTAAGGACTCAGAGTCACATTTCTTTGTTTAATTCCCTCAGAAACACAGTGGTTCAGCCCCTAACCCGCGCCGTTCTTGAGATGGTGCTGCTGCCTCTCACTTTTCAGATCCCCAAGTTCAATCAAGTCTCTAGGTTTTGTATGGCCATGAAGAAGCCGGAAGAAACAGTTGTAGCTTCAAGTGATCGCCATGAGATGAGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGTTCCACTGTGATAACTGCTGTTACTGCACAGAACACCGTGGGGGTTCAGGATGTAAACATTATACCGGAGGGCTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAAAACAGGAATGCTACCTTCTACTGGCATCATTCAGGCAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGGGACGTTCTGGCTTGTCCTACAATTATTTCAGTGTTACAGGATGAACTTCTACCAATGGCTGACTTGGTAACCCCAAATTTGAAGGAAGCATCTGCCTTACTTGGCGACCCATCGAATCTTCTCCCCCCTGCCTCCTCAACAATCGCTTGCCATCCTGACCCCGATCCCTTCTTCCTTTCCCTCCTCATCAGCCAATTAATCTACCTCATTGCATTTACCAATTGGCCTCAAGACGACTAA

Coding sequence (CDS)

ATGGTGCTGCTGCCTCTCACTTTTCAGATCCCCAAGTTCAATCAAGTCTCTAGGTTTTGTATGGCCATGAAGAAGCCGGAAGAAACAGTTGTAGCTTCAAGTGATCGCCATGAGATGAGGATTCCGCATGTGTTGAGTGTTGCTGGTTCTGATTCAGGAGCAGGGGCTGGAATCCAAGCGGATCTTAAGACTTGTGCTGCTCGTGGAGTGTACTGTTCCACTGTGATAACTGCTGTTACTGCACAGAACACCGTGGGGGTTCAGGATGTAAACATTATACCGGAGGGCTTTGTTTCAGAGCAGCTGAAATCTGTTCTCTCTGATATGCAAGTGGATGTGGTGAAAACAGGAATGCTACCTTCTACTGGCATCATTCAGGCAGCTTTGGTGGTTGATCCTGTCATGGTGTCTACCAGTGGGGACGTTCTGGCTTGTCCTACAATTATTTCAGTGTTACAGGATGAACTTCTACCAATGGCTGACTTGGTAACCCCAAATTTGAAGGAAGCATCTGCCTTACTTGGCGACCCATCGAATCTTCTCCCCCCTGCCTCCTCAACAATCGCTTGCCATCCTGACCCCGATCCCTTCTTCCTTTCCCTCCTCATCAGCCAATTAATCTACCTCATTGCATTTACCAATTGGCCTCAAGACGACTAA
Homology
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BLAST of CmoCh04G019850 vs. Swiss-Prot
Match: TPS1L_ARATH (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana GN=TH1 PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 6.2e-45
Identity = 99/146 (67.81%), Postives = 120/146 (82.19%), Query Frame = 1 

Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVS 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITAVTAQNT GVQ V+++P  F+S
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 EQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSGDVLACPTI 159
           EQLKSVLSD + DVVKTGMLPST I++            ALVVDPVMVSTSG VLA  +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQDELLPMADLVTPNLKEASALL 175
           +S+ ++ LLP+AD++TPN+KEASALL
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALL 174

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BLAST of CmoCh04G019850 vs. Swiss-Prot
Match: TPS1_ORYSJ (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 6.2e-45
Identity = 100/158 (63.29%), Postives = 120/158 (75.95%), Query Frame = 1 

Query: 32  ASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVN 91
           ++S   EM  PHVL+VAGSDSG GAGIQAD+K CAA G YCS+V+TAVTAQNT GVQ ++
Sbjct: 47  SASAAREMPWPHVLTVAGSDSGGGAGIQADIKACAALGAYCSSVVTAVTAQNTAGVQGIH 106

Query: 92  IIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSG 151
           ++PE F+ EQL SVLSDM VDVVKTGMLPS G+++            ALVVDPVMVSTSG
Sbjct: 107 VVPEEFIREQLNSVLSDMSVDVVKTGMLPSIGVVRVLCESLKKFPVKALVVDPVMVSTSG 166

Query: 152 DVLACPTIISVLQDELLPMADLVTPNLKEASALLGDPS 179
           D L+  + +SV +DEL  MAD+VTPN+KEAS LLG  S
Sbjct: 167 DTLSESSTLSVYRDELFAMADIVTPNVKEASRLLGGVS 204

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BLAST of CmoCh04G019850 vs. Swiss-Prot
Match: TPS1_BRANA (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus GN=BTH1 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.2e-41
Identity = 94/147 (63.95%), Postives = 116/147 (78.91%), Query Frame = 1 

Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVS 99
           ++  VL+VAGSDSGAGAGIQAD+K CAARGVYC++V TAV A+NT  VQ V+++P   VS
Sbjct: 31  KVAQVLTVAGSDSGAGAGIQADIKVCAARGVYCASVKTAVKAKNTRAVQSVHLLPPDSVS 90

Query: 100 EQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSGDVLACPTI 159
           EQLKSVLSD +VDVVKTGMLPS  I++            ALVVDPVMVSTSG VLA  +I
Sbjct: 91  EQLKSVLSDFEVDVVKTGMLPSPEIVEVLLQNLSEYPVRALVVDPVMVSTSGHVLAGSSI 150

Query: 160 ISVLQDELLPMADLVTPNLKEASALLG 176
           +S+ ++ LLP+AD++TPN+KEASALLG
Sbjct: 151 LSIFRERLLPLADIITPNVKEASALLG 177

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BLAST of CmoCh04G019850 vs. Swiss-Prot
Match: THIED_GEOSL (Thiamine biosynthesis bifunctional protein ThiED OS=Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) GN=thiDE PE=3 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.9e-26
Identity = 76/143 (53.15%), Postives = 94/143 (65.73%), Query Frame = 1 

Query: 44  VLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLK 103
           VL+VAGSDSG GAGIQADLKT    G Y S+V+TA+TAQNT GV  ++ +P  FV++QL 
Sbjct: 228 VLTVAGSDSGGGAGIQADLKTVTLLGSYGSSVLTALTAQNTRGVSGIHGVPPAFVADQLD 287

Query: 104 SVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSGDVLACPTIISVL 163
           +V SD+ VDVVKTGML S   I A            +VVDPVMV+  G  L     +SVL
Sbjct: 288 AVFSDIPVDVVKTGMLFSAETIVAIAAKLTEYRRRMVVVDPVMVAKGGANLIDRGAVSVL 347

Query: 164 QDELLPMADLVTPNLKEASALLG 176
           ++ L P+A LVTPN+ EA  L G
Sbjct: 348 KERLFPLAYLVTPNIPEAERLTG 370

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BLAST of CmoCh04G019850 vs. Swiss-Prot
Match: THID_RHIME (Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS=Rhizobium meliloti (strain 1021) GN=thiD PE=3 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 8.4e-26
Identity = 74/141 (52.48%), Postives = 92/141 (65.25%), Query Frame = 1 

Query: 45  LSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKS 104
           LS+AGSDSG GAGIQADLKT +A GVY ++VITA+TAQNT GV  V  +    VS Q+ +
Sbjct: 6   LSIAGSDSGGGAGIQADLKTFSALGVYGASVITAITAQNTRGVTAVEDVSAEIVSAQMDA 65

Query: 105 VLSDMQVDVVKTGMLPSTGIIQA----------ALVVDPVMVSTSGDVLACPTIISVLQD 164
           V SD+ V  VK GM+     I A            VVDPVMV+TSGD L  P  ++ L +
Sbjct: 66  VFSDLDVKAVKIGMVSRRETIAAIADGLRRFGKRAVVDPVMVATSGDALLRPDAVAALIE 125

Query: 165 ELLPMADLVTPNLKEASALLG 176
           ELLP+A +VTPNL EA+ + G
Sbjct: 126 ELLPLALVVTPNLAEAALMTG 146

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BLAST of CmoCh04G019850 vs. TrEMBL
Match: A0A067KRT8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04989 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.5e-53
Identity = 119/155 (76.77%), Postives = 133/155 (85.81%), Query Frame = 1 

Query: 32  ASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVN 91
           AS+D  + +IPHVL+VAGSDSGAGAGIQADLKTCAARGV+CSTVITAVTAQNTVGVQ VN
Sbjct: 8   ASNDASKAKIPHVLTVAGSDSGAGAGIQADLKTCAARGVFCSTVITAVTAQNTVGVQGVN 67

Query: 92  IIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQ-----------AALVVDPVMVSTSG 151
           I+PE FV+EQLKSVLSDMQVDVVKTGMLPS GI+             ALVVDPVMVSTSG
Sbjct: 68  IVPEDFVAEQLKSVLSDMQVDVVKTGMLPSIGIVNILRQSLREFPVRALVVDPVMVSTSG 127

Query: 152 DVLACPTIISVLQDELLPMADLVTPNLKEASALLG 176
           DVLA P+I+S  Q+ELLPMAD++TPN+KEASALLG
Sbjct: 128 DVLAGPSILSCFQEELLPMADIITPNIKEASALLG 162

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BLAST of CmoCh04G019850 vs. TrEMBL
Match: A0A161ZLW2_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_025347 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.1e-52
Identity = 118/155 (76.13%), Postives = 133/155 (85.81%), Query Frame = 1 

Query: 32  ASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVN 91
           A+S+  + R+PHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQ VN
Sbjct: 27  ATSESIKERMPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQGVN 86

Query: 92  IIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSG 151
           I+PE FVSEQLKSVLSDM V VVKTGMLP+ GI++            ALVVDPVMVSTSG
Sbjct: 87  IVPEKFVSEQLKSVLSDMNVTVVKTGMLPTVGIVKVLNDSLKQFPVQALVVDPVMVSTSG 146

Query: 152 DVLACPTIISVLQDELLPMADLVTPNLKEASALLG 176
           DVLA P+I+ +L+++LLPMAD+VTPNLKEASALLG
Sbjct: 147 DVLAGPSILDILREQLLPMADIVTPNLKEASALLG 181

[Close]
BLAST of CmoCh04G019850 vs. TrEMBL
Match: W9S3N5_9ROSA (Thiamine biosynthesis bifunctional protein ThiED OS=Morus notabilis GN=L484_016718 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 2.8e-52
Identity = 116/175 (66.29%), Postives = 139/175 (79.43%), Query Frame = 1 

Query: 12  KFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVY 71
           + N+  +   +M+   +    + D   +++PHVL+VAGSDSGAGAGIQADLK CAARGVY
Sbjct: 36  RINEDKQIHTSMEMQRDNGSITKDHSRLKVPHVLTVAGSDSGAGAGIQADLKACAARGVY 95

Query: 72  CSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQA---- 131
           CSTVITAVTAQNTVGVQ  NI+PE FV++QL+SVLSDMQVDVVKTGMLPSTGI++     
Sbjct: 96  CSTVITAVTAQNTVGVQGGNIVPEDFVAKQLRSVLSDMQVDVVKTGMLPSTGIVKVLRQS 155

Query: 132 -------ALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLG 176
                   LVVDPVMVSTSGDVLA P+I++V ++ELLPMAD+VTPNLKEASALLG
Sbjct: 156 LAEFPVRGLVVDPVMVSTSGDVLAGPSILTVFREELLPMADIVTPNLKEASALLG 210

[Close]
BLAST of CmoCh04G019850 vs. TrEMBL
Match: D7TGZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.8e-51
Identity = 115/148 (77.70%), Postives = 128/148 (86.49%), Query Frame = 1 

Query: 39  MRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFV 98
           M+IPHVL+VAGSDSGAGAGIQADLK CAARGVYCSTVITAVTAQNTVGVQ VNI+PE FV
Sbjct: 1   MKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVNIVPEDFV 60

Query: 99  SEQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSGDVLACPT 158
           +EQLKSVLSDM VDVVKTGMLP+ GI++            ALVVDPVMVSTSGDVLA P+
Sbjct: 61  AEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALVVDPVMVSTSGDVLAGPS 120

Query: 159 IISVLQDELLPMADLVTPNLKEASALLG 176
           I++  ++ELLPMAD+VTPNLKEASALLG
Sbjct: 121 ILAAFREELLPMADIVTPNLKEASALLG 148

[Close]
BLAST of CmoCh04G019850 vs. TrEMBL
Match: A0A161X4G3_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006692 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 9.0e-51
Identity = 112/149 (75.17%), Postives = 127/149 (85.23%), Query Frame = 1 

Query: 38  EMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGF 97
           + R+PHVL+VAGSDSGAGAGIQADLK CAARGVYCSTV+TAVTAQNTVGVQ VNI+PE F
Sbjct: 29  KQRLPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVVTAVTAQNTVGVQGVNIVPERF 88

Query: 98  VSEQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSGDVLACP 157
           VSEQL+SVLSDM VDVVKTGMLP+ GI++A           ALVVDPVMVSTSGDVLA P
Sbjct: 89  VSEQLQSVLSDMNVDVVKTGMLPTVGIVKALYDRLKQYPVQALVVDPVMVSTSGDVLAGP 148

Query: 158 TIISVLQDELLPMADLVTPNLKEASALLG 176
           +++   +D+LLPMAD+ TPNLKEASALLG
Sbjct: 149 SVLDTFRDQLLPMADIATPNLKEASALLG 177

[Close]
BLAST of CmoCh04G019850 vs. TAIR10
Match: AT1G22940.1 (AT1G22940.1 thiamin biosynthesis protein, putative)

HSP 1 Score: 182.2 bits (461), Expect = 3.5e-46
Identity = 99/146 (67.81%), Postives = 120/146 (82.19%), Query Frame = 1 

Query: 40  RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVS 99
           ++P VL+VAGSDSGAGAGIQADLK CAARGVYC++VITAVTAQNT GVQ V+++P  F+S
Sbjct: 29  KVPQVLTVAGSDSGAGAGIQADLKVCAARGVYCASVITAVTAQNTRGVQSVHLLPPEFIS 88

Query: 100 EQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------ALVVDPVMVSTSGDVLACPTI 159
           EQLKSVLSD + DVVKTGMLPST I++            ALVVDPVMVSTSG VLA  +I
Sbjct: 89  EQLKSVLSDFEFDVVKTGMLPSTEIVEVLLQNLSDFPVRALVVDPVMVSTSGHVLAGSSI 148

Query: 160 ISVLQDELLPMADLVTPNLKEASALL 175
           +S+ ++ LLP+AD++TPN+KEASALL
Sbjct: 149 LSIFRERLLPIADIITPNVKEASALL 174

[Close]
BLAST of CmoCh04G019850 vs. NCBI nr
Match: gi|659120551|ref|XP_008460243.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-75
Identity = 161/186 (86.56%), Postives = 167/186 (89.78%), Query Frame = 1 

Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60
           MV LPL  QIPKFNQVSRFCMAMKK EE VVASSDR+E RIPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITA+TAQNTVGVQDVNI+PEGFVS+QLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQA-----------ALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 176
           STGI+Q            ALVVDPVMVSTSGDVLA PTIISVLQ+ELLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKE 180

[Close]
BLAST of CmoCh04G019850 vs. NCBI nr
Match: gi|449445302|ref|XP_004140412.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis sativus])

HSP 1 Score: 283.1 bits (723), Expect = 4.1e-73
Identity = 156/187 (83.42%), Postives = 166/187 (88.77%), Query Frame = 1 

Query: 1   MVLLPLTFQIPKFNQVSRFCMAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQA 60
           MV LPL  QIPKFNQVSRFCMAMKK +E VVASS+R+E  IPHVLSVAGSDSGAGAGIQA
Sbjct: 1   MVPLPLISQIPKFNQVSRFCMAMKKQDEMVVASSNRNETCIPHVLSVAGSDSGAGAGIQA 60

Query: 61  DLKTCAARGVYCSTVITAVTAQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLP 120
           DLKTCAARGVYCSTVITA+TAQNTVGVQDVN++PEGFVS+QLKSVLSDMQVDVVKTGMLP
Sbjct: 61  DLKTCAARGVYCSTVITAITAQNTVGVQDVNVVPEGFVSKQLKSVLSDMQVDVVKTGMLP 120

Query: 121 STGIIQA-----------ALVVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKE 177
           STGI+Q            ALVVDPVMVSTSGDVLA P IISVLQ++LLPMADLVTPNLKE
Sbjct: 121 STGIVQVLHQCLKEFPVRALVVDPVMVSTSGDVLAGPAIISVLQEKLLPMADLVTPNLKE 180

[Close]
BLAST of CmoCh04G019850 vs. NCBI nr
Match: gi|659120553|ref|XP_008460244.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 260.0 bits (663), Expect = 3.7e-66
Identity = 144/166 (86.75%), Postives = 150/166 (90.36%), Query Frame = 1 

Query: 21  MAMKKPEETVVASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVT 80
           MAMKK EE VVASSDR+E RIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITA+T
Sbjct: 1   MAMKKQEEMVVASSDRYETRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAIT 60

Query: 81  AQNTVGVQDVNIIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQA-----------AL 140
           AQNTVGVQDVNI+PEGFVS+QLKSVLSDMQVDVVKTGMLPSTGI+Q            AL
Sbjct: 61  AQNTVGVQDVNIVPEGFVSKQLKSVLSDMQVDVVKTGMLPSTGIVQVLHQCLKEFPVRAL 120

Query: 141 VVDPVMVSTSGDVLACPTIISVLQDELLPMADLVTPNLKEASALLG 176
           VVDPVMVSTSGDVLA PTIISVLQ+ELLPMADLVTPNLKEASALLG
Sbjct: 121 VVDPVMVSTSGDVLAGPTIISVLQEELLPMADLVTPNLKEASALLG 166

[Close]
BLAST of CmoCh04G019850 vs. NCBI nr
Match: gi|643731588|gb|KDP38832.1| (hypothetical protein JCGZ_04989 [Jatropha curcas])

HSP 1 Score: 217.6 bits (553), Expect = 2.1e-53
Identity = 119/155 (76.77%), Postives = 133/155 (85.81%), Query Frame = 1 

Query: 32  ASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVN 91
           AS+D  + +IPHVL+VAGSDSGAGAGIQADLKTCAARGV+CSTVITAVTAQNTVGVQ VN
Sbjct: 8   ASNDASKAKIPHVLTVAGSDSGAGAGIQADLKTCAARGVFCSTVITAVTAQNTVGVQGVN 67

Query: 92  IIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQ-----------AALVVDPVMVSTSG 151
           I+PE FV+EQLKSVLSDMQVDVVKTGMLPS GI+             ALVVDPVMVSTSG
Sbjct: 68  IVPEDFVAEQLKSVLSDMQVDVVKTGMLPSIGIVNILRQSLREFPVRALVVDPVMVSTSG 127

Query: 152 DVLACPTIISVLQDELLPMADLVTPNLKEASALLG 176
           DVLA P+I+S  Q+ELLPMAD++TPN+KEASALLG
Sbjct: 128 DVLAGPSILSCFQEELLPMADIITPNIKEASALLG 162

[Close]
BLAST of CmoCh04G019850 vs. NCBI nr
Match: gi|802591932|ref|XP_012071303.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Jatropha curcas])

HSP 1 Score: 217.6 bits (553), Expect = 2.1e-53
Identity = 119/155 (76.77%), Postives = 133/155 (85.81%), Query Frame = 1 

Query: 32  ASSDRHEMRIPHVLSVAGSDSGAGAGIQADLKTCAARGVYCSTVITAVTAQNTVGVQDVN 91
           AS+D  + +IPHVL+VAGSDSGAGAGIQADLKTCAARGV+CSTVITAVTAQNTVGVQ VN
Sbjct: 66  ASNDASKAKIPHVLTVAGSDSGAGAGIQADLKTCAARGVFCSTVITAVTAQNTVGVQGVN 125

Query: 92  IIPEGFVSEQLKSVLSDMQVDVVKTGMLPSTGIIQ-----------AALVVDPVMVSTSG 151
           I+PE FV+EQLKSVLSDMQVDVVKTGMLPS GI+             ALVVDPVMVSTSG
Sbjct: 126 IVPEDFVAEQLKSVLSDMQVDVVKTGMLPSIGIVNILRQSLREFPVRALVVDPVMVSTSG 185

Query: 152 DVLACPTIISVLQDELLPMADLVTPNLKEASALLG 176
           DVLA P+I+S  Q+ELLPMAD++TPN+KEASALLG
Sbjct: 186 DVLAGPSILSCFQEELLPMADIITPNIKEASALLG 220

The following BLAST results are available for this feature:
BLAST of CmoCh04G019850 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TPS1L_ARATH6.2e-4567.81Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal... [more]
TPS1_ORYSJ6.2e-4563.29Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ... [more]
TPS1_BRANA4.2e-4163.95Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ... [more]
THIED_GEOSL2.9e-2653.15Thiamine biosynthesis bifunctional protein ThiED OS=Geobacter sulfurreducens (st... [more]
THID_RHIME8.4e-2652.48Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase OS=Rhizobium meliloti (st... [more]
BLAST of CmoCh04G019850 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A067KRT8_JATCU1.5e-5376.77Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04989 PE=3 SV=1[more]
A0A161ZLW2_DAUCA2.1e-5276.13Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_025347 PE=4 SV=1[more]
W9S3N5_9ROSA2.8e-5266.29Thiamine biosynthesis bifunctional protein ThiED OS=Morus notabilis GN=L484_0167... [more]
D7TGZ0_VITVI1.8e-5177.70Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=... [more]
A0A161X4G3_DAUCA9.0e-5175.17Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_006692 PE=4 SV=1[more]
BLAST of CmoCh04G019850 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G22940.13.5e-4667.81 thiamin biosynthesis protein, putative[more]
BLAST of CmoCh04G019850 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659120551|ref|XP_008460243.1|1.5e-7586.56PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|449445302|ref|XP_004140412.1|4.1e-7383.42PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis... [more]
gi|659120553|ref|XP_008460244.1|3.7e-6686.75PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|643731588|gb|KDP38832.1|2.1e-5376.77hypothetical protein JCGZ_04989 [Jatropha curcas][more]
gi|802591932|ref|XP_012071303.1|2.1e-5376.77PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013749PM/HMP-P_kinase-1
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009228 thiamine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005575 cellular_component
molecular_function GO:0008972 phosphomethylpyrimidine kinase activity
molecular_function GO:0004789 thiamine-phosphate diphosphorylase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G019850.1CmoCh04G019850.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013749Pyridoxamine kinase/Phosphomethylpyrimidine kinasePFAMPF08543Phos_pyr_kincoord: 51..177
score: 5.3
NoneNo IPR availablePANTHERPTHR20858PHOSPHOMETHYLPYRIMIDINE KINASEcoord: 28..176
score: 1.1
NoneNo IPR availablePANTHERPTHR20858:SF17HYDROXYMETHYLPYRIMIDINE/PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATEDcoord: 28..176
score: 1.1

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh04G019850CSPI05G12990Wild cucumber (PI 183967)cmocpiB740
CmoCh04G019850Lsi11G000210Bottle gourd (USVL1VR-Ls)cmolsiB621
CmoCh04G019850Cp4.1LG01g16710Cucurbita pepo (Zucchini)cmocpeB673
CmoCh04G019850CsaV3_5G012700Cucumber (Chinese Long) v3cmocucB0884
CmoCh04G019850Cla97C11G207240Watermelon (97103) v2cmowmbB710
CmoCh04G019850CsGy5G009580Cucumber (Gy14) v2cgybcmoB661
CmoCh04G019850Carg25920Silver-seed gourdcarcmoB0317
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh04G019850Wax gourdcmowgoB0906
CmoCh04G019850Wax gourdcmowgoB0914
CmoCh04G019850Cucurbita maxima (Rimu)cmacmoB728
CmoCh04G019850Cucumber (Chinese Long) v2cmocuB732
CmoCh04G019850Bottle gourd (USVL1VR-Ls)cmolsiB623