Csa5G262270 (gene) Cucumber (Chinese Long) v2

Overview
NameCsa5G262270
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionThiamine-phosphate synthase; contains IPR003733 (Thiamine phosphate synthase)
LocationChr5 : 10996766 .. 10997441 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCGTGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTAATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGATGAAATGTTTGAATAATTTTGTAATGCTTTGCTTTTTGTAGAAAGCCTTTTGAAATAAAAATAATTGTCACAATATATATGTCATGTAAGATTCTATTCATATAAAGTAGAGCATATTTATATTCGCTATAATAAGCATTCTGAT

mRNA sequence

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCGTGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTAATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGA

Coding sequence (CDS)

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCGTGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTAATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGA

Protein sequence

MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT*
Homology
[Close]
BLAST of Csa5G262270 vs. Swiss-Prot
Match: TPS1L_ARATH (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana GN=TH1 PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 1.3e-69
Identity = 131/172 (76.16%), Postives = 145/172 (84.30%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AK+CI ICR+HGV LLINDRIDIALAC+ADGVHVGQSD+P   VR L
Sbjct: 347 REKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSL 406

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN T+G+DGLK VC ASKLP
Sbjct: 407 LGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA +VM I  PNLKGVAVVSALFD+ CVL +A KLH TL E+
Sbjct: 467 VVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLHKTLKES 518

[Close]
BLAST of Csa5G262270 vs. Swiss-Prot
Match: TPS1_ORYSJ (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 1.4e-68
Identity = 125/173 (72.25%), Postives = 145/173 (83.82%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKD++TR+FLE AK+C++IC++ GVPLLINDR+DIALACNADGVHVGQ D+ AHEVR L
Sbjct: 374 REKDSETREFLEAAKACMEICKSSGVPLLINDRVDIALACNADGVHVGQLDMSAHEVREL 433

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT  QA+QAW DGADYIGCGGV+PT+TKANN T+G DGLK VCLASKLP
Sbjct: 434 LGPGKIIGVSCKTPAQAQQAWNDGADYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLP 493

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 176
           VVAIGGIN +NA +VM +G+PNLKGVAVVSALFDR  V+ E   + + L   +
Sbjct: 494 VVAIGGINASNAGSVMELGLPNLKGVAVVSALFDRPSVVAETRNMKSILTNTS 546

[Close]
BLAST of Csa5G262270 vs. Swiss-Prot
Match: TPS1_BRANA (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus GN=BTH1 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.2e-67
Identity = 127/172 (73.84%), Postives = 145/172 (84.30%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AKSC+ ICR++GV LLINDR DIA+A +ADGVHVGQSD+P   VR L
Sbjct: 348 REKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALDADGVHVGQSDMPVDLVRSL 407

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN T+G+DGL+ VC ASKLP
Sbjct: 408 LGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLREVCKASKLP 467

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA +VM IG PNLKGVAVVSALFD++CVL +A KLH TL E+
Sbjct: 468 VVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQAKKLHKTLTES 519

[Close]
BLAST of Csa5G262270 vs. Swiss-Prot
Match: THIE_ALKOO (Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) GN=thiE PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.5e-36
Identity = 79/169 (46.75%), Postives = 111/169 (65.68%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A + +F ++A     +   + VPL+INDR+DIALA +ADGVHVGQSD+PAH VR +
Sbjct: 43  REKEASSLEFYQLALKAKALTEKYNVPLIINDRVDIALAVDADGVHVGQSDLPAHIVRSM 102

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           +G NKI+GVS  T E++++A  DGADYIG G ++PT TK +   V +D L+ +     +P
Sbjct: 103 IGQNKILGVSTATLEESKKAAEDGADYIGVGALFPTGTKTDANPVTLDQLRYIKENMDIP 162

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATL 172
           VV IGGI   N   +M +GI    GVA+VSA+  ++ + E A  L A++
Sbjct: 163 VVGIGGICEDNIKTIMEVGI---DGVAIVSAILGKENIKEAAESLKASI 208

[Close]
BLAST of Csa5G262270 vs. Swiss-Prot
Match: THIE_CLOTE (Thiamine-phosphate synthase OS=Clostridium tetani (strain Massachusetts / E88) GN=thiE PE=3 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 1.0e-34
Identity = 72/165 (43.64%), Postives = 109/165 (66.06%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+  +++F E+AK    I   + +PL+INDR+DIALA NA+G+H+GQ D+PA+ VR++
Sbjct: 42  REKNVSSKEFYEIAKDIKVITDKYNIPLIINDRVDIALAVNAEGIHIGQKDLPANIVRKI 101

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           +G +KI+GVS  T E A +A  DGADY+G G ++PTN+K +  +  I+ LK +  A  +P
Sbjct: 102 IGEDKILGVSANTIEDALKAQRDGADYLGVGAIFPTNSKKDAESTSIETLKEIKNAVNIP 161

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKL 168
           +VAIGGIN  N   V  +   N+ G+AV+S +  ++ V +   +L
Sbjct: 162 IVAIGGINENN---VQKLKETNIDGIAVISTILGKEDVKKACEEL 203

[Close]
BLAST of Csa5G262270 vs. TrEMBL
Match: A0A0A0KPF9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G262270 PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 6.3e-95
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1 

Query: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120
           RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK
Sbjct: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 176

[Close]
BLAST of Csa5G262270 vs. TrEMBL
Match: D7TGZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 6.5e-76
Identity = 140/172 (81.40%), Postives = 154/172 (89.53%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDA+TRDFLE AK+C++IC +HGVPLLINDRID+ALAC+ADGVHVGQSDIPA  VR L
Sbjct: 321 REKDAETRDFLEAAKACVEICHSHGVPLLINDRIDVALACDADGVHVGQSDIPARVVRTL 380

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT EQAE+AWIDGADYIGCGGVYPTNTKANN+TVG+DGLK VCLASKLP
Sbjct: 381 LGPEKIIGVSCKTPEQAEKAWIDGADYIGCGGVYPTNTKANNITVGLDGLKTVCLASKLP 440

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGIN +NA  VM IG+PNLKGVAVVSALFDR+CVL E  KLH  L +A
Sbjct: 441 VVAIGGINASNARTVMEIGVPNLKGVAVVSALFDRECVLTETQKLHGDLTQA 492

[Close]
BLAST of Csa5G262270 vs. TrEMBL
Match: M5XRD0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003609mg PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 1.5e-75
Identity = 140/173 (80.92%), Postives = 153/173 (88.44%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKD +TRDF+E AKSC++ICRAHGVPLLINDRID+ALAC+ADGVH+GQSD+PAH  R L
Sbjct: 386 REKDIETRDFVEAAKSCLQICRAHGVPLLINDRIDVALACDADGVHIGQSDMPAHTARAL 445

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT EQAEQAWI GADYIGCGGVYPTNTKANNLTVG+DGLK VCLASKLP
Sbjct: 446 LGPEKIIGVSCKTPEQAEQAWIAGADYIGCGGVYPTNTKANNLTVGLDGLKTVCLASKLP 505

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 176
           VVAIGGI  +NA  VM IG+PNLKGVAVVSA+FD +CVL E  KLHA L EAT
Sbjct: 506 VVAIGGIKVSNARPVMEIGVPNLKGVAVVSAIFDSECVLTETRKLHAVLTEAT 558

[Close]
BLAST of Csa5G262270 vs. TrEMBL
Match: A0A061E190_THECC (Thiamin biosynthesis protein, putative isoform 3 OS=Theobroma cacao GN=TCM_006934 PE=3 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 3.6e-74
Identity = 137/172 (79.65%), Postives = 153/172 (88.95%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDA+T+DFLE AK+C+KIC +HGVPLLINDRIDIALAC+ADGVHVGQSD+PAH  R L
Sbjct: 214 REKDAETQDFLESAKACLKICHSHGVPLLINDRIDIALACDADGVHVGQSDMPAHVARTL 273

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT EQA+QAW+DGADYIGCGGVYPTNTK NN+TVG+DGLK VC+ASKLP
Sbjct: 274 LGPEKIIGVSCKTPEQAQQAWVDGADYIGCGGVYPTNTKENNITVGLDGLKTVCMASKLP 333

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA  VM IG+PNLKGVAVVSALFDR+CVL E  KLHA L E+
Sbjct: 334 VVAIGGIGVSNARIVMEIGVPNLKGVAVVSALFDRECVLTETRKLHAMLSES 385

[Close]
BLAST of Csa5G262270 vs. TrEMBL
Match: A0A061DZ91_THECC (Thiamin biosynthesis protein, putative isoform 2 OS=Theobroma cacao GN=TCM_006934 PE=3 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 3.6e-74
Identity = 137/172 (79.65%), Postives = 153/172 (88.95%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDA+T+DFLE AK+C+KIC +HGVPLLINDRIDIALAC+ADGVHVGQSD+PAH  R L
Sbjct: 242 REKDAETQDFLESAKACLKICHSHGVPLLINDRIDIALACDADGVHVGQSDMPAHVARTL 301

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT EQA+QAW+DGADYIGCGGVYPTNTK NN+TVG+DGLK VC+ASKLP
Sbjct: 302 LGPEKIIGVSCKTPEQAQQAWVDGADYIGCGGVYPTNTKENNITVGLDGLKTVCMASKLP 361

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA  VM IG+PNLKGVAVVSALFDR+CVL E  KLHA L E+
Sbjct: 362 VVAIGGIGVSNARIVMEIGVPNLKGVAVVSALFDRECVLTETRKLHAMLSES 413

[Close]
BLAST of Csa5G262270 vs. TAIR10
Match: AT1G22940.1 (AT1G22940.1 thiamin biosynthesis protein, putative)

HSP 1 Score: 263.8 bits (673), Expect = 7.4e-71
Identity = 131/172 (76.16%), Postives = 145/172 (84.30%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AK+CI ICR+HGV LLINDRIDIALAC+ADGVHVGQSD+P   VR L
Sbjct: 347 REKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSL 406

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP+KIIGVSCKT EQA QAW DGADYIG GGV+PTNTKANN T+G+DGLK VC ASKLP
Sbjct: 407 LGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGI  +NA +VM I  PNLKGVAVVSALFD+ CVL +A KLH TL E+
Sbjct: 467 VVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLHKTLKES 518

[Close]
BLAST of Csa5G262270 vs. NCBI nr
Match: gi|700195612|gb|KGN50789.1| (hypothetical protein Csa_5G262270 [Cucumis sativus])

HSP 1 Score: 354.8 bits (909), Expect = 9.0e-95
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1 

Query: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120
           RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK
Sbjct: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 176

[Close]
BLAST of Csa5G262270 vs. NCBI nr
Match: gi|449445302|ref|XP_004140412.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis sativus])

HSP 1 Score: 350.5 bits (898), Expect = 1.7e-93
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 419 LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 532

[Close]
BLAST of Csa5G262270 vs. NCBI nr
Match: gi|659120553|ref|XP_008460244.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 340.5 bits (872), Expect = 1.8e-90
Identity = 168/174 (96.55%), Postives = 171/174 (98.28%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 339 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 398

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 399 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 459 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 512

[Close]
BLAST of Csa5G262270 vs. NCBI nr
Match: gi|659120551|ref|XP_008460243.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 340.5 bits (872), Expect = 1.8e-90
Identity = 168/174 (96.55%), Postives = 171/174 (98.28%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGPNK+IGVSCKT EQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP
Sbjct: 419 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 177
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 532

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BLAST of Csa5G262270 vs. NCBI nr
Match: gi|297739580|emb|CBI29762.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 291.6 bits (745), Expect = 9.4e-76
Identity = 140/172 (81.40%), Postives = 154/172 (89.53%), Query Frame = 1 

Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDA+TRDFLE AK+C++IC +HGVPLLINDRID+ALAC+ADGVHVGQSDIPA  VR L
Sbjct: 321 REKDAETRDFLEAAKACVEICHSHGVPLLINDRIDVALACDADGVHVGQSDIPARVVRTL 380

Query: 63  LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 122
           LGP KIIGVSCKT EQAE+AWIDGADYIGCGGVYPTNTKANN+TVG+DGLK VCLASKLP
Sbjct: 381 LGPEKIIGVSCKTPEQAEKAWIDGADYIGCGGVYPTNTKANNITVGLDGLKTVCLASKLP 440

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 175
           VVAIGGIN +NA  VM IG+PNLKGVAVVSALFDR+CVL E  KLH  L +A
Sbjct: 441 VVAIGGINASNARTVMEIGVPNLKGVAVVSALFDRECVLTETQKLHGDLTQA 492

The following BLAST results are available for this feature:
BLAST of Csa5G262270 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TPS1L_ARATH1.3e-6976.16Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal... [more]
TPS1_ORYSJ1.4e-6872.25Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ... [more]
TPS1_BRANA1.2e-6773.84Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ... [more]
THIE_ALKOO5.5e-3646.75Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) GN=thiE P... [more]
THIE_CLOTE1.0e-3443.64Thiamine-phosphate synthase OS=Clostridium tetani (strain Massachusetts / E88) G... [more]
BLAST of Csa5G262270 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KPF9_CUCSA6.3e-95100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G262270 PE=3 SV=1[more]
D7TGZ0_VITVI6.5e-7681.40Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=... [more]
M5XRD0_PRUPE1.5e-7580.92Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003609mg PE=3 SV=1[more]
A0A061E190_THECC3.6e-7479.65Thiamin biosynthesis protein, putative isoform 3 OS=Theobroma cacao GN=TCM_00693... [more]
A0A061DZ91_THECC3.6e-7479.65Thiamin biosynthesis protein, putative isoform 2 OS=Theobroma cacao GN=TCM_00693... [more]
BLAST of Csa5G262270 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G22940.17.4e-7176.16 thiamin biosynthesis protein, putative[more]
BLAST of Csa5G262270 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700195612|gb|KGN50789.1|9.0e-95100.00hypothetical protein Csa_5G262270 [Cucumis sativus][more]
gi|449445302|ref|XP_004140412.1|1.7e-93100.00PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis... [more]
gi|659120553|ref|XP_008460244.1|1.8e-9096.55PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|659120551|ref|XP_008460243.1|1.8e-9096.55PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|297739580|emb|CBI29762.3|9.4e-7681.40unnamed protein product [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003733Thiamine phosphate synthase
IPR013785Aldolase_TIM
IPR022998ThiamineP_synth_TenI
Vocabulary: Molecular Function
TermDefinition
GO:0004789thiamine-phosphate diphosphorylase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0009228thiamine biosynthetic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009228 thiamine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005575 cellular_component
molecular_function GO:0008902 hydroxymethylpyrimidine kinase activity
molecular_function GO:0008972 phosphomethylpyrimidine kinase activity
molecular_function GO:0004789 thiamine-phosphate diphosphorylase activity
molecular_function GO:0003824 catalytic activity
Unigene
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU126613cucumber EST collection version 3.0transcribed_cluster
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa5G262270.1Csa5G262270.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU126613CU126613transcribed_cluster


InterPro
Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003733Thiamine phosphate synthaseHAMAPMF_00097TMP_synthasecoord: 2..173
score: 19
IPR003733Thiamine phosphate synthasePFAMPF02581TMP-TENIcoord: 3..154
score: 2.0
IPR003733Thiamine phosphate synthaseTIGRFAMsTIGR00693TIGR00693coord: 3..162
score: 2.7
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 3..171
score: 8.6
IPR022998Thiamin phosphate synthase superfamilyunknownSSF51391Thiamin phosphate synthasecoord: 2..168
score: 5.89
NoneNo IPR availablePANTHERPTHR20857THIAMINE-PHOSPHATE PYROPHOSPHORYLASEcoord: 3..167
score: 4.1
NoneNo IPR availablePANTHERPTHR20857:SF15THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYMEcoord: 3..167
score: 4.1