CmoCh04G019860 (gene) Cucurbita moschata (Rifu)

Overview
NameCmoCh04G019860
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
DescriptionThiamine-phosphate synthase
LocationCmo_Chr04 : 10285108 .. 10288364 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTGAAGAGCGTTATGTTTGTTTTTAGAAATGTACTTGTCAAAGGCGGGGACCTTCCAGATTCATTGGATGCTGTAGATATATTCTTTGATGGTAAGTACAGTACAGGTTGCATAATTCTGACAATTTGATATATATATTTTTGTTATGAAGGAAGCACATCCACTGAACCCATATTTTTTTCATAGGCAAGGATCTGCATGAGCTACGATCTTCACGCATAACGACTCGCAACACTCATGGCACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAGCTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGGTAATTATCTACGGACTCTTATTTTAAACCCACCAATAAGAAGATTAAAGTCTCAACGGTTCATAATACAACTTCCTATTGAATAGTTAAATAATTTTAAAAAAATAAACTTATGAGCCATCTTCAATTTGTTATAGGATAATAGTTGTTGAAAATAACGCTATATTCACTATGTATGCTCACTGACCTTGACGGATGATCAAGACGGGATGTTAAGAGTTACTTTAATGCTTTTTTTTTTTTTTTTTTTTTTCTGGCTTTTCATTGAAATGATGAAAAATAAATAAATGTTCTAGGGATACAAACTCCCAAAGGGAGTGAAAAAGGGAAAAAAGAAAAAAAAAATACAAACAGTACATACAACCAAATAGAGATTAGTCATGAAAATTAATAAATTTTTAAGGAACCAAGAACTTTCCCTGAACGCTTGGAGAGCTTGCCAATGAATAATGAAACTGTGAATAAAACCTCTATCAAACACTGAAAATCAATAGATGCTTCAAAACTTCAAAAAGAAAACCAAGAATCGACCACAACCAACAACTCTTGAGCCAATTTTACTCGTGACACCTTCATCAACTCTTGAGCTTTGCAAGAATTACTAACCATTGTTAAAAGCTTTGTAAGAACGATCACCGACAAAGAAATAAAAAATATCCTCATGCAAGTCAATTCAAATATTTCATGCAAGTAGATTCAAATATCCCAGTGTAGACAAAAAAAAAAACTTTGTAAAGCAAAACCATGATGTGGCTACCCTCTCGAGCTAATGGAGAGTCTTCTTGTCACAGTCCTAAAATAATAAACCAAAAGTTTAAGAAAAAGAATCTACAAGAACATCAGCCTCCCTACTGAAATCCATGGATGAATGAGTGATTCCACACTGTTCAAACGTACCTGGGAGTCAACCTCAAACACGTCATTACATGGTGGCGAGTCCTTGTCGAGTGAATAACTCCTCTCGTTAAACCTTGAGTGAAGTCAAAATGGTTGTTGAGGGGATAAGTGAAGGGGGAGGGTGGTAAGGCGACATTCTAATTGAATAAGAATTTAAAGAAGTTTCCTGGAAGCATGAATAGTAAGAAGAAAAAAGCACATCTTTTTTGTGAGAATAATGCAAATGATAGGCTGGAATATATAAACATTCTTCCCCTCCTTTTTAACACTTGTGGGCTTGGAAATCTCAATCCGTAATGGAGGCCTCTAGGATTCTCAATATATGAGGGATATATCTTTCACAGTCCTATTTGATAATCAAGCTTTTAAAAGTTGTACTTATTTTTTCACCCTTCTTTACAGCATCTTTCCTAATGAAACATTTAAATTCACGACCAAATTCTATTTACAAACCCAAGGTTTTCAAAACTACTTTTTTTAGCACTAATCAAAATATGGTTGCCACTCAATCCAATTTGGAGCTTCAAATTCTTTGGTTATTTGTTTTTTTTTTCATTTTTTTTTGGATGAAAGAGACGTATCATTTCACTCCATATATATAATTTGAGTAATTCAAAACGCAATAGGCTTGGTTGTCGTTACCAGACTTGATGATTGTCCACAACTAAGGTTTTATTTATCTGGATCTTTACAGATAAGCAAACAGTTTATTGAAAGAGCATTGAGTTACAGCAAGGACATTAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTTAAGAGTCGAGAACAAAGTTCGTATAGACAGGGGAGTTTCAATCAATCTGACTTATTCTTGTATGCTGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTTCTATCACAGATGCTGTTAAAGCTGCAGTTGAAGGAGGTGCTACTATAATTCAGATAAGGTTTGTCTACTGTTTTCCATTAATCAATTGTTGTTAGTTTGTTCGCTTAAAAATGTTTTCAGAAGTTCATACATATTCAAGTCATTACTTCAGATGTATTGAATTCCTTGTTGCATTTTAATACCTCTTAGCACCTTCTCAGTTGTTCAAAGGTTGAATGGCTTTAGAAACTTACCCATAAAGTGTAGGCTAAAGGCTCCCTATAGGCTAGCAATAGTGAGGTAGTAATCTTGGGTTAATAGAACCCGACTGTAAGATTTGAATCTCTTAACGTGTTGTATTATTGTTGACCGAAGTTATAAACTTAGTATAATTAAAACTTGTATTCAATTGTTAATTTGCTTCCAATGGTCGCATGATCTTATTTTTATGAACAGGGAAAAGGATGCTAAAACTCGCGATTTCTTGGAAGCAGCCAAATCATGTATAGAGATTTGTCACACACACGGCGTTCCATTACTGATCAACGATCGTATTGACGTTGCACTTGCCTGTGGTGCTGATGGTGTACACATTGGTCAGTCCGATATTCCTGTTCATGCAGCTCGTAGCCTTCTTGGCCCTGATAAGATTATCGGTGTCTCATGCAAGACACCGGAGCAAGCGGAACAGGCATGGCTTGATGGTGCTGATTACATTGGGTGTGGTGGAGTTTATCCCACAAACACGAAGGCAAACAATCTGACTGTTGGGCTTGATGGATTGAAAAGGGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGAATTAATCACAGTAATGCAGCGGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCGGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGACATTGAAGCTACATGCAACATTAGTGGAGGCTACAGCATTATCACCTGCGAAATGAGTGAGGGACCATAGTGCAACGACACAGTTTCTTGATTTTGATGAAATCAAATGTATGAATAATTGTAATGCTTTGATTTTAGTAGAAAGGTTTTTGAAATAAATGTTAGTCTCAATGTGATTATAATGTAACAAAATTTAATTTCATGATAATAAGCATGGTCATTATATATTCTTT

mRNA sequence

ATGCTGAAGAGCGTTATGTTTGTTTTTAGAAATGTACTTGTCAAAGGCGGGGACCTTCCAGATTCATTGGATGCTGTAGATATATTCTTTGATGGCAAGGATCTGCATGAGCTACGATCTTCACGCATAACGACTCGCAACACTCATGGCACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAGCTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGATAAGCAAACAGTTTATTGAAAGAGCATTGAGTTACAGCAAGGACATTAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTTAAGAGTCGAGAACAAAGTTCGTATAGACAGGGGAGTTTCAATCAATCTGACTTATTCTTGTATGCTGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTTCTATCACAGATGCTGTTAAAGCTGCAGTTGAAGGAGGTGCTACTATAATTCAGATAAGGGAAAAGGATGCTAAAACTCGCGATTTCTTGGAAGCAGCCAAATCATGTATAGAGATTTGTCACACACACGGCGTTCCATTACTGATCAACGATCGTATTGACGTTGCACTTGCCTGTGGTGCTGATGGTGTACACATTGGTCAGTCCGATATTCCTGTTCATGCAGCTCGTAGCCTTCTTGGCCCTGATAAGATTATCGGTGTCTCATGCAAGACACCGGAGCAAGCGGAACAGGCATGGCTTGATGGTGCTGATTACATTGGGTGTGGTGGAGTTTATCCCACAAACACGAAGGCAAACAATCTGACTGTTGGGCTTGATGGATTGAAAAGGGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGAATTAATCACAGTAATGCAGCGGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCGGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGACATTGAAGCTACATGCAACATTAGTGGAGGCTACAGCATTATCACCTGCGAAATGAGTGAGGGACCATAGTGCAACGACACAGTTTCTTGATTTTGATGAAATCAAATGTATGAATAATTGTAATGCTTTGATTTTAGTAGAAAGGTTTTTGAAATAAATGTTAGTCTCAATGTGATTATAATGTAACAAAATTTAATTTCATGATAATAAGCATGGTCATTATATATTCTTT

Coding sequence (CDS)

ATGCTGAAGAGCGTTATGTTTGTTTTTAGAAATGTACTTGTCAAAGGCGGGGACCTTCCAGATTCATTGGATGCTGTAGATATATTCTTTGATGGCAAGGATCTGCATGAGCTACGATCTTCACGCATAACGACTCGCAACACTCATGGCACTGGATGCAGCTTAGCATCATGCATTGCAGCTGAGCTTGCTAAAGGGTCTTCAATGTTCTCAGCTGTTAAGATAAGCAAACAGTTTATTGAAAGAGCATTGAGTTACAGCAAGGACATTAGCATTGGAAATGGACCTCAAGGCCCATTCGATCATCTATGTCGTCTTAAGAGTCGAGAACAAAGTTCGTATAGACAGGGGAGTTTCAATCAATCTGACTTATTCTTGTATGCTGTTACGGACTCGGGTATGAATAAGCGTTGGGACCGTTCTATCACAGATGCTGTTAAAGCTGCAGTTGAAGGAGGTGCTACTATAATTCAGATAAGGGAAAAGGATGCTAAAACTCGCGATTTCTTGGAAGCAGCCAAATCATGTATAGAGATTTGTCACACACACGGCGTTCCATTACTGATCAACGATCGTATTGACGTTGCACTTGCCTGTGGTGCTGATGGTGTACACATTGGTCAGTCCGATATTCCTGTTCATGCAGCTCGTAGCCTTCTTGGCCCTGATAAGATTATCGGTGTCTCATGCAAGACACCGGAGCAAGCGGAACAGGCATGGCTTGATGGTGCTGATTACATTGGGTGTGGTGGAGTTTATCCCACAAACACGAAGGCAAACAATCTGACTGTTGGGCTTGATGGATTGAAAAGGGTTTGCTTAGCTTCCAAGTTGCCTGTGGTTGCAATTGGTGGAATTAATCACAGTAATGCAGCGGCTGTGATGGAAATTGGTGTCCCAAATCTTAAAGGTGTTGCAGTTGTGTCGGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGACATTGAAGCTACATGCAACATTAGTGGAGGCTACAGCATTATCACCTGCGAAATGA
Homology
[Close]
BLAST of CmoCh04G019860 vs. Swiss-Prot
Match: TPS1L_ARATH (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana GN=TH1 PE=1 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-136
Identity = 242/322 (75.16%), Postives = 274/322 (85.09%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           R VLVKGGDLPDS D+VD++FDGK+ HELRS RI TRNTHGTGC+LASCIAAELAKGSSM
Sbjct: 198 RFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSM 257

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK++K+F++ AL YSKDI IG+G QGPFDH   LK   QSS R   FN  DLFLYAV
Sbjct: 258 LSAVKVAKRFVDNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSS-RCSIFNPDDLFLYAV 317

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDS MNK+W+RSI DA+KAA+EGGATIIQ+REK+A+TR+FLE AK+CI+IC +HGV LLI
Sbjct: 318 TDSRMNKKWNRSIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLI 377

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSD+PV   RSLLGPDKIIGVSCKTPEQA QAW DGADYIG 
Sbjct: 378 NDRIDIALACDADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGS 437

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGV+PTNTKANN T+GLDGLK VC ASKLPVVAIGGI  SNA +VM+I  PNLKGVAVVS
Sbjct: 438 GGVFPTNTKANNRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVS 497

Query: 310 ALFDRQCVLEETLKLHATLVEA 332
           ALFD+ CVL +  KLH TL E+
Sbjct: 498 ALFDQDCVLTQAKKLHKTLKES 518

[Close]
BLAST of CmoCh04G019860 vs. Swiss-Prot
Match: TPS1_BRANA (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus GN=BTH1 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 2.5e-133
Identity = 234/322 (72.67%), Postives = 272/322 (84.47%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           R VLVKGGDLPDS D+VD++FDG + HEL S RI TRNTHGTGC+LASCIAAELAKGS+M
Sbjct: 200 RFVLVKGGDLPDSSDSVDVYFDGNEFHELHSPRIATRNTHGTGCTLASCIAAELAKGSNM 259

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK++K+F++ AL+YSKDI IG+G QGPFDH   LK  +  SYRQ +F   DLFLYAV
Sbjct: 260 LSAVKVAKRFVDSALNYSKDIVIGSGMQGPFDHFLSLK--DPQSYRQSTFKPDDLFLYAV 319

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDS MNK+W+RSI DAVKAA+EGGATIIQ+REK+A+TR+FLE AKSC++IC ++GV LLI
Sbjct: 320 TDSRMNKKWNRSIVDAVKAAIEGGATIIQLREKEAETREFLEEAKSCVDICRSNGVCLLI 379

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDR D+A+A  ADGVH+GQSD+PV   RSLLGPDKIIGVSCKT EQA QAW DGADYIG 
Sbjct: 380 NDRFDIAIALDADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTQEQAHQAWKDGADYIGS 439

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGV+PTNTKANN T+GLDGL+ VC ASKLPVVAIGGI  SNA +VM IG PNLKGVAVVS
Sbjct: 440 GGVFPTNTKANNRTIGLDGLREVCKASKLPVVAIGGIGISNAESVMRIGEPNLKGVAVVS 499

Query: 310 ALFDRQCVLEETLKLHATLVEA 332
           ALFD++CVL +  KLH TL E+
Sbjct: 500 ALFDQECVLTQAKKLHKTLTES 519

[Close]
BLAST of CmoCh04G019860 vs. Swiss-Prot
Match: TPS1_ORYSJ (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 456.1 bits (1172), Expect = 3.4e-127
Identity = 219/323 (67.80%), Postives = 266/323 (82.35%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           ++VLVKGGD+ +S DA D+FFDGK+  EL + RI T NTHGTGC+LASCIA+ELAKG++M
Sbjct: 224 KHVLVKGGDMLESSDATDVFFDGKEFIELHAHRIKTHNTHGTGCTLASCIASELAKGATM 283

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
             AV+++K F+E AL +SKD+ +GNGPQGPFDHL +LK    +   Q SF    LFLYAV
Sbjct: 284 LHAVQVAKNFVESALHHSKDLVVGNGPQGPFDHLFKLKCPPYNVGSQPSFKPDQLFLYAV 343

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMNK+W RSI +AV+AA+EGGATI+Q+REKD++TR+FLEAAK+C+EIC + GVPLLI
Sbjct: 344 TDSGMNKKWGRSIKEAVQAAIEGGATIVQLREKDSETREFLEAAKACMEICKSSGVPLLI 403

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDR+D+ALAC ADGVH+GQ D+  H  R LLGP KIIGVSCKTP QA+QAW DGADYIGC
Sbjct: 404 NDRVDIALACNADGVHVGQLDMSAHEVRELLGPGKIIGVSCKTPAQAQQAWNDGADYIGC 463

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGV+PT+TKANN T+G DGLK VCLASKLPVVAIGGIN SNA +VME+G+PNLKGVAVVS
Sbjct: 464 GGVFPTSTKANNPTLGFDGLKTVCLASKLPVVAIGGINASNAGSVMELGLPNLKGVAVVS 523

Query: 310 ALFDRQCVLEETLKLHATLVEAT 333
           ALFDR  V+ ET  + + L   +
Sbjct: 524 ALFDRPSVVAETRNMKSILTNTS 546

[Close]
BLAST of CmoCh04G019860 vs. Swiss-Prot
Match: THIE_CLOAB (Thiamine-phosphate synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) GN=thiE PE=3 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.6e-42
Identity = 92/198 (46.46%), Postives = 132/198 (66.67%), Query Frame = 1 

Query: 123 DLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHT 182
           D  LY VTD  + K  +R +  +++ A++GG T++Q+REK+  T DF E+A    +I  T
Sbjct: 5   DYKLYLVTDRKVLK--ERDLYKSIEEAIKGGVTLVQLREKEMSTLDFYESALKLKKITET 64

Query: 183 HGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLD 242
           + +PL+INDRID+ALA  ADGVHIGQSD+P+  AR LLG DKIIGVS  + E+A +A  +
Sbjct: 65  YKIPLIINDRIDIALAINADGVHIGQSDMPLIKARELLGKDKIIGVSAHSIEEALEAERN 124

Query: 243 GADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNL 302
           GA Y+G G +Y T+TK +   V L+ LK +  + K+PVV IGGIN  NA  V+E GV   
Sbjct: 125 GATYLGVGAIYNTSTKGDAQAVSLEELKNIKNSVKIPVVGIGGINEENANKVIETGV--- 184

Query: 303 KGVAVVSALFDRQCVLEE 321
            G++V+S +   Q + ++
Sbjct: 185 DGISVISGILSAQKIKDK 197

[Close]
BLAST of CmoCh04G019860 vs. Swiss-Prot
Match: THIE_ALKOO (Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) GN=thiE PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.4e-41
Identity = 89/206 (43.20%), Postives = 132/206 (64.08%), Query Frame = 1 

Query: 123 DLFLYAVTDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHT 182
           D  LY V+D  + K   R    +++ A+ GGATI+Q+REK+A + +F + A     +   
Sbjct: 8   DYGLYLVSDRDVLK--GRDFIKSLEEAILGGATIVQLREKEASSLEFYQLALKAKALTEK 67

Query: 183 HGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLD 242
           + VPL+INDR+D+ALA  ADGVH+GQSD+P H  RS++G +KI+GVS  T E++++A  D
Sbjct: 68  YNVPLIINDRVDIALAVDADGVHVGQSDLPAHIVRSMIGQNKILGVSTATLEESKKAAED 127

Query: 243 GADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNL 302
           GADYIG G ++PT TK +   V LD L+ +     +PVV IGGI   N   +ME+G+   
Sbjct: 128 GADYIGVGALFPTGTKTDANPVTLDQLRYIKENMDIPVVGIGGICEDNIKTIMEVGI--- 187

Query: 303 KGVAVVSALFDRQCVLEETLKLHATL 329
            GVA+VSA+  ++ + E    L A++
Sbjct: 188 DGVAIVSAILGKENIKEAAESLKASI 208

[Close]
BLAST of CmoCh04G019860 vs. TrEMBL
Match: D7TGZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 2.2e-149
Identity = 261/325 (80.31%), Postives = 293/325 (90.15%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           RNVLVKGGDLP SLDAVDIFFDG D +ELRSSRI TRNTHGTGC+LASCIAAELAKGS +
Sbjct: 171 RNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKGSQI 230

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK +K +IE AL YSKDI+IGNG QGPFDHL +LKS  ++S+R+ +FN ++LFLYAV
Sbjct: 231 LSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFLYAV 290

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMNK+W RSIT+AVKAA+EGGATI+Q+REKDA+TRDFLEAAK+C+EICH+HGVPLLI
Sbjct: 291 TDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVPLLI 350

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRIDVALAC ADGVH+GQSDIP    R+LLGP+KIIGVSCKTPEQAE+AW+DGADYIGC
Sbjct: 351 NDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADYIGC 410

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANN+TVGLDGLK VCLASKLPVVAIGGIN SNA  VMEIGVPNLKGVAVVS
Sbjct: 411 GGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVAVVS 470

Query: 310 ALFDRQCVLEETLKLHATLVEATAL 335
           ALFDR+CVL ET KLH  L +A A+
Sbjct: 471 ALFDRECVLTETQKLHGDLTQAAAM 495

[Close]
BLAST of CmoCh04G019860 vs. TrEMBL
Match: M5XRD0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003609mg PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 6.5e-149
Identity = 264/324 (81.48%), Postives = 289/324 (89.20%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           RNVLVKGGDLPDSLDAVDIFFDG+ L+ELRSSRI TRNTHGTGC+LASCIAAELAKG+SM
Sbjct: 236 RNVLVKGGDLPDSLDAVDIFFDGEHLYELRSSRIKTRNTHGTGCTLASCIAAELAKGASM 295

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
             AVK++K F+E AL YSK+I IGNGPQGPFDHL +LKS   +S RQ  FN SDLFLYAV
Sbjct: 296 LEAVKVAKCFVETALDYSKEIFIGNGPQGPFDHLMKLKSNAHNSGRQVRFNPSDLFLYAV 355

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMN+RW  SI+DAVKAAV+GGATI+Q+REKD +TRDF+EAAKSC++IC  HGVPLLI
Sbjct: 356 TDSGMNRRWGHSISDAVKAAVQGGATIVQLREKDIETRDFVEAAKSCLQICRAHGVPLLI 415

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRIDVALAC ADGVHIGQSD+P H AR+LLGP+KIIGVSCKTPEQAEQAW+ GADYIGC
Sbjct: 416 NDRIDVALACDADGVHIGQSDMPAHTARALLGPEKIIGVSCKTPEQAEQAWIAGADYIGC 475

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVGLDGLK VCLASKLPVVAIGGI  SNA  VMEIGVPNLKGVAVVS
Sbjct: 476 GGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIKVSNARPVMEIGVPNLKGVAVVS 535

Query: 310 ALFDRQCVLEETLKLHATLVEATA 334
           A+FD +CVL ET KLHA L EATA
Sbjct: 536 AIFDSECVLTETRKLHAVLTEATA 559

[Close]
BLAST of CmoCh04G019860 vs. TrEMBL
Match: A0A067EGP5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010260mg PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 5.1e-146
Identity = 257/322 (79.81%), Postives = 287/322 (89.13%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           R VLVKGGDLPDS DAVDIFFDG+D HELRSSR+ TRNTHGTGC+LASCIAAELAKGS M
Sbjct: 188 RTVLVKGGDLPDSSDAVDIFFDGEDFHELRSSRVNTRNTHGTGCTLASCIAAELAKGSPM 247

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK++K F+E AL YSKDI IG+GPQGPFDHL RLKS  + S+R  +FN SDLFLYAV
Sbjct: 248 LSAVKVAKCFVETALDYSKDIVIGSGPQGPFDHLLRLKSTSRQSHRAEAFNPSDLFLYAV 307

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMNK+W RSITDAVKAA+EGGATIIQ+REKDA TR FLEAAK+C++IC  HGVPLLI
Sbjct: 308 TDSGMNKKWGRSITDAVKAALEGGATIIQLREKDADTRGFLEAAKACLQICCVHGVPLLI 367

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSD+P   AR+LLGPDKIIGVSCKTPE+A QAW+DGA+YIGC
Sbjct: 368 NDRIDIALACDADGVHLGQSDMPARTARALLGPDKIIGVSCKTPEEAHQAWIDGANYIGC 427

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVGLDGLK VCLASKLPVVAIGGI  SNA+ VM+IGV NLKGVAVVS
Sbjct: 428 GGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIGISNASDVMKIGVSNLKGVAVVS 487

Query: 310 ALFDRQCVLEETLKLHATLVEA 332
           ALFDR+C+L E+ KLHA L++A
Sbjct: 488 ALFDRECILPESKKLHAVLMDA 509

[Close]
BLAST of CmoCh04G019860 vs. TrEMBL
Match: V4T678_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 3.3e-145
Identity = 256/322 (79.50%), Postives = 285/322 (88.51%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           R VLVKGGDLPDS DAVDIFFDG+D HELRSSR+ TRNTHGTGC+LASCIAAELAKGS M
Sbjct: 216 RTVLVKGGDLPDSSDAVDIFFDGEDFHELRSSRVNTRNTHGTGCTLASCIAAELAKGSPM 275

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK++K F+E AL YSKDI IG+GPQGPFDHL RLKS    S+R  +FN SDLFLYAV
Sbjct: 276 LSAVKVAKCFVETALDYSKDIVIGSGPQGPFDHLLRLKSTSHQSHRAEAFNPSDLFLYAV 335

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSG NK+W RSITDAVKAA+EGGATIIQ+REKDA TR FLEAAK+C++IC  HGVPLLI
Sbjct: 336 TDSGTNKKWGRSITDAVKAALEGGATIIQLREKDADTRGFLEAAKACLQICCVHGVPLLI 395

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSD+P   AR+LLGPDKIIGVSCKTPE+A QAW+DGA+YIGC
Sbjct: 396 NDRIDIALACDADGVHLGQSDMPARTARALLGPDKIIGVSCKTPEEAHQAWIDGANYIGC 455

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVGLDGLK VCLASKLPVVAIGGI  SNA+ VM+IGV NLKGVAVVS
Sbjct: 456 GGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIGISNASDVMKIGVSNLKGVAVVS 515

Query: 310 ALFDRQCVLEETLKLHATLVEA 332
           ALFDR+C+L E+ KLHA L++A
Sbjct: 516 ALFDRECILPESKKLHAVLMDA 537

[Close]
BLAST of CmoCh04G019860 vs. TrEMBL
Match: V4T108_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 3.3e-145
Identity = 256/322 (79.50%), Postives = 285/322 (88.51%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           R VLVKGGDLPDS DAVDIFFDG+D HELRSSR+ TRNTHGTGC+LASCIAAELAKGS M
Sbjct: 188 RTVLVKGGDLPDSSDAVDIFFDGEDFHELRSSRVNTRNTHGTGCTLASCIAAELAKGSPM 247

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK++K F+E AL YSKDI IG+GPQGPFDHL RLKS    S+R  +FN SDLFLYAV
Sbjct: 248 LSAVKVAKCFVETALDYSKDIVIGSGPQGPFDHLLRLKSTSHQSHRAEAFNPSDLFLYAV 307

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSG NK+W RSITDAVKAA+EGGATIIQ+REKDA TR FLEAAK+C++IC  HGVPLLI
Sbjct: 308 TDSGTNKKWGRSITDAVKAALEGGATIIQLREKDADTRGFLEAAKACLQICCVHGVPLLI 367

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSD+P   AR+LLGPDKIIGVSCKTPE+A QAW+DGA+YIGC
Sbjct: 368 NDRIDIALACDADGVHLGQSDMPARTARALLGPDKIIGVSCKTPEEAHQAWIDGANYIGC 427

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVGLDGLK VCLASKLPVVAIGGI  SNA+ VM+IGV NLKGVAVVS
Sbjct: 428 GGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIGISNASDVMKIGVSNLKGVAVVS 487

Query: 310 ALFDRQCVLEETLKLHATLVEA 332
           ALFDR+C+L E+ KLHA L++A
Sbjct: 488 ALFDRECILPESKKLHAVLMDA 509

[Close]
BLAST of CmoCh04G019860 vs. TAIR10
Match: AT1G22940.1 (AT1G22940.1 thiamin biosynthesis protein, putative)

HSP 1 Score: 487.6 bits (1254), Expect = 6.0e-138
Identity = 242/322 (75.16%), Postives = 274/322 (85.09%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           R VLVKGGDLPDS D+VD++FDGK+ HELRS RI TRNTHGTGC+LASCIAAELAKGSSM
Sbjct: 198 RFVLVKGGDLPDSSDSVDVYFDGKEFHELRSPRIATRNTHGTGCTLASCIAAELAKGSSM 257

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK++K+F++ AL YSKDI IG+G QGPFDH   LK   QSS R   FN  DLFLYAV
Sbjct: 258 LSAVKVAKRFVDNALDYSKDIVIGSGMQGPFDHFFGLKKDPQSS-RCSIFNPDDLFLYAV 317

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDS MNK+W+RSI DA+KAA+EGGATIIQ+REK+A+TR+FLE AK+CI+IC +HGV LLI
Sbjct: 318 TDSRMNKKWNRSIVDALKAAIEGGATIIQLREKEAETREFLEEAKACIDICRSHGVSLLI 377

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSD+PV   RSLLGPDKIIGVSCKTPEQA QAW DGADYIG 
Sbjct: 378 NDRIDIALACDADGVHVGQSDMPVDLVRSLLGPDKIIGVSCKTPEQAHQAWKDGADYIGS 437

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGV+PTNTKANN T+GLDGLK VC ASKLPVVAIGGI  SNA +VM+I  PNLKGVAVVS
Sbjct: 438 GGVFPTNTKANNRTIGLDGLKEVCEASKLPVVAIGGIGISNAGSVMQIDAPNLKGVAVVS 497

Query: 310 ALFDRQCVLEETLKLHATLVEA 332
           ALFD+ CVL +  KLH TL E+
Sbjct: 498 ALFDQDCVLTQAKKLHKTLKES 518

[Close]
BLAST of CmoCh04G019860 vs. NCBI nr
Match: gi|659120553|ref|XP_008460244.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 587.4 bits (1513), Expect = 1.6e-164
Identity = 287/323 (88.85%), Postives = 305/323 (94.43%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           +NVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRI +RNTHGTGCSLASCI+AELAKGSSM
Sbjct: 189 KNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSM 248

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
           FSAVK SKQFIERAL YSKDI+IG+GPQGPFDHLCRLKSREQSSY QG FN +DLFLYAV
Sbjct: 249 FSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDLFLYAV 308

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMN+RWDRSITDAVKAAVEGGATI+QIREKDAKTRDFLE AKSCI+ICH HGVPLLI
Sbjct: 309 TDSGMNERWDRSITDAVKAAVEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHGVPLLI 368

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSDIP H  R LLGP+K+IGVSCKT EQAEQAW+DGADYIGC
Sbjct: 369 NDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGADYIGC 428

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVG+DGLKRVCLASKLPVVAIGGINH+NAAAVM IG+PNL+GVAVVS
Sbjct: 429 GGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRGVAVVS 488

Query: 310 ALFDRQCVLEETLKLHATLVEAT 333
           ALFDRQCVLE   KLHATLVEAT
Sbjct: 489 ALFDRQCVLEAASKLHATLVEAT 511

[Close]
BLAST of CmoCh04G019860 vs. NCBI nr
Match: gi|659120551|ref|XP_008460243.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 587.4 bits (1513), Expect = 1.6e-164
Identity = 287/323 (88.85%), Postives = 305/323 (94.43%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           +NVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRI +RNTHGTGCSLASCI+AELAKGSSM
Sbjct: 209 KNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCISAELAKGSSM 268

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
           FSAVK SKQFIERAL YSKDI+IG+GPQGPFDHLCRLKSREQSSY QG FN +DLFLYAV
Sbjct: 269 FSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCRLKSREQSSYSQGCFNPTDLFLYAV 328

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMN+RWDRSITDAVKAAVEGGATI+QIREKDAKTRDFLE AKSCI+ICH HGVPLLI
Sbjct: 329 TDSGMNERWDRSITDAVKAAVEGGATIVQIREKDAKTRDFLEVAKSCIKICHAHGVPLLI 388

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSDIP H  R LLGP+K+IGVSCKT EQAEQAW+DGADYIGC
Sbjct: 389 NDRIDIALACDADGVHVGQSDIPAHEVRRLLGPNKVIGVSCKTMEQAEQAWIDGADYIGC 448

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVG+DGLKRVCLASKLPVVAIGGINH+NAAAVM IG+PNL+GVAVVS
Sbjct: 449 GGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLRGVAVVS 508

Query: 310 ALFDRQCVLEETLKLHATLVEAT 333
           ALFDRQCVLE   KLHATLVEAT
Sbjct: 509 ALFDRQCVLEAASKLHATLVEAT 531

[Close]
BLAST of CmoCh04G019860 vs. NCBI nr
Match: gi|449445302|ref|XP_004140412.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis sativus])

HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-162
Identity = 285/323 (88.24%), Postives = 302/323 (93.50%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           +NVL+KGGDLPDSLDAVDIFFDGKDLHELRSSRI +RNTHGTGCSLASCIAAELAKGSSM
Sbjct: 209 KNVLIKGGDLPDSLDAVDIFFDGKDLHELRSSRIKSRNTHGTGCSLASCIAAELAKGSSM 268

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
           FSAVK SKQFIERAL YSKDI+IG+GPQGPFDHLC LK+RE SSY QG FN +DLFLYAV
Sbjct: 269 FSAVKASKQFIERALRYSKDINIGHGPQGPFDHLCCLKNREPSSYSQGCFNPADLFLYAV 328

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMN+RWDRSITDAVK AVEGGATI+QIREKDAKTRDFLE AKSCI+IC  HGVPLLI
Sbjct: 329 TDSGMNERWDRSITDAVKDAVEGGATIVQIREKDAKTRDFLEVAKSCIKICRAHGVPLLI 388

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRID+ALAC ADGVH+GQSDIP H  R LLGP+KIIGVSCKT EQAEQAW+DGADYIGC
Sbjct: 389 NDRIDIALACNADGVHVGQSDIPAHEVRRLLGPNKIIGVSCKTTEQAEQAWIDGADYIGC 448

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANNLTVG+DGLKRVCLASKLPVVAIGGINH+NAAAVM IG+PNLKGVAVVS
Sbjct: 449 GGVYPTNTKANNLTVGIDGLKRVCLASKLPVVAIGGINHTNAAAVMGIGIPNLKGVAVVS 508

Query: 310 ALFDRQCVLEETLKLHATLVEAT 333
           ALFDRQCVLEE  KLHATLVEAT
Sbjct: 509 ALFDRQCVLEEASKLHATLVEAT 531

[Close]
BLAST of CmoCh04G019860 vs. NCBI nr
Match: gi|731411629|ref|XP_010658056.1| (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Vitis vinifera])

HSP 1 Score: 536.6 bits (1381), Expect = 3.2e-149
Identity = 261/325 (80.31%), Postives = 293/325 (90.15%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           RNVLVKGGDLP SLDAVDIFFDG D +ELRSSRI TRNTHGTGC+LASCIAAELAKGS +
Sbjct: 239 RNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKGSQI 298

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK +K +IE AL YSKDI+IGNG QGPFDHL +LKS  ++S+R+ +FN ++LFLYAV
Sbjct: 299 LSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFLYAV 358

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMNK+W RSIT+AVKAA+EGGATI+Q+REKDA+TRDFLEAAK+C+EICH+HGVPLLI
Sbjct: 359 TDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVPLLI 418

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRIDVALAC ADGVH+GQSDIP    R+LLGP+KIIGVSCKTPEQAE+AW+DGADYIGC
Sbjct: 419 NDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADYIGC 478

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANN+TVGLDGLK VCLASKLPVVAIGGIN SNA  VMEIGVPNLKGVAVVS
Sbjct: 479 GGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVAVVS 538

Query: 310 ALFDRQCVLEETLKLHATLVEATAL 335
           ALFDR+CVL ET KLH  L +A A+
Sbjct: 539 ALFDRECVLTETQKLHGDLTQAAAM 563

[Close]
BLAST of CmoCh04G019860 vs. NCBI nr
Match: gi|297739580|emb|CBI29762.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 536.6 bits (1381), Expect = 3.2e-149
Identity = 261/325 (80.31%), Postives = 293/325 (90.15%), Query Frame = 1 

Query: 10  RNVLVKGGDLPDSLDAVDIFFDGKDLHELRSSRITTRNTHGTGCSLASCIAAELAKGSSM 69
           RNVLVKGGDLP SLDAVDIFFDG D +ELRSSRI TRNTHGTGC+LASCIAAELAKGS +
Sbjct: 171 RNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKGSQI 230

Query: 70  FSAVKISKQFIERALSYSKDISIGNGPQGPFDHLCRLKSREQSSYRQGSFNQSDLFLYAV 129
            SAVK +K +IE AL YSKDI+IGNG QGPFDHL +LKS  ++S+R+ +FN ++LFLYAV
Sbjct: 231 LSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFLYAV 290

Query: 130 TDSGMNKRWDRSITDAVKAAVEGGATIIQIREKDAKTRDFLEAAKSCIEICHTHGVPLLI 189
           TDSGMNK+W RSIT+AVKAA+EGGATI+Q+REKDA+TRDFLEAAK+C+EICH+HGVPLLI
Sbjct: 291 TDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVPLLI 350

Query: 190 NDRIDVALACGADGVHIGQSDIPVHAARSLLGPDKIIGVSCKTPEQAEQAWLDGADYIGC 249
           NDRIDVALAC ADGVH+GQSDIP    R+LLGP+KIIGVSCKTPEQAE+AW+DGADYIGC
Sbjct: 351 NDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADYIGC 410

Query: 250 GGVYPTNTKANNLTVGLDGLKRVCLASKLPVVAIGGINHSNAAAVMEIGVPNLKGVAVVS 309
           GGVYPTNTKANN+TVGLDGLK VCLASKLPVVAIGGIN SNA  VMEIGVPNLKGVAVVS
Sbjct: 411 GGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVAVVS 470

Query: 310 ALFDRQCVLEETLKLHATLVEATAL 335
           ALFDR+CVL ET KLH  L +A A+
Sbjct: 471 ALFDRECVLTETQKLHGDLTQAAAM 495

The following BLAST results are available for this feature:
BLAST of CmoCh04G019860 vs. Swiss-Prot
Match NameE-valueIdentityDescription
TPS1L_ARATH1.1e-13675.16Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal... [more]
TPS1_BRANA2.5e-13372.67Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ... [more]
TPS1_ORYSJ3.4e-12767.80Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ... [more]
THIE_CLOAB2.6e-4246.46Thiamine-phosphate synthase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM... [more]
THIE_ALKOO6.4e-4143.20Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) GN=thiE P... [more]
BLAST of CmoCh04G019860 vs. TrEMBL
Match NameE-valueIdentityDescription
D7TGZ0_VITVI2.2e-14980.31Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0035g00320 PE=3 SV=... [more]
M5XRD0_PRUPE6.5e-14981.48Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003609mg PE=3 SV=1[more]
A0A067EGP5_CITSI5.1e-14679.81Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g010260mg PE=3 SV=1[more]
V4T678_9ROSI3.3e-14579.50Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1[more]
V4T108_9ROSI3.3e-14579.50Uncharacterized protein OS=Citrus clementina GN=CICLE_v10000782mg PE=3 SV=1[more]
BLAST of CmoCh04G019860 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G22940.16.0e-13875.16 thiamin biosynthesis protein, putative[more]
BLAST of CmoCh04G019860 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659120553|ref|XP_008460244.1|1.6e-16488.85PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|659120551|ref|XP_008460243.1|1.6e-16488.85PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|449445302|ref|XP_004140412.1|1.5e-16288.24PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis... [more]
gi|731411629|ref|XP_010658056.1|3.2e-14980.31PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ... [more]
gi|297739580|emb|CBI29762.3|3.2e-14980.31unnamed protein product [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003733Thiamine phosphate synthase
IPR013749PM/HMP-P_kinase-1
IPR013785Aldolase_TIM
IPR022998ThiamineP_synth_TenI
Vocabulary: Molecular Function
TermDefinition
GO:0004789thiamine-phosphate diphosphorylase activity
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0009228thiamine biosynthetic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009228 thiamine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0008902 hydroxymethylpyrimidine kinase activity
molecular_function GO:0008972 phosphomethylpyrimidine kinase activity
molecular_function GO:0004789 thiamine-phosphate diphosphorylase activity
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G019860.1CmoCh04G019860.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003733Thiamine phosphate synthaseHAMAPMF_00097TMP_synthasecoord: 123..330
score: 27
IPR003733Thiamine phosphate synthasePFAMPF02581TMP-TENIcoord: 126..311
score: 1.5
IPR003733Thiamine phosphate synthaseTIGRFAMsTIGR00693TIGR00693coord: 126..315
score: 1.3
IPR013749Pyridoxamine kinase/Phosphomethylpyrimidine kinasePFAMPF08543Phos_pyr_kincoord: 10..96
score: 9.0
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 119..327
score: 7.0
IPR022998Thiamin phosphate synthase superfamilyunknownSSF51391Thiamin phosphate synthasecoord: 122..324
score: 1.83
NoneNo IPR availablePANTHERPTHR20857THIAMINE-PHOSPHATE PYROPHOSPHORYLASEcoord: 113..315
score: 1.5
NoneNo IPR availablePANTHERPTHR20857:SF15THIAMINE BIOSYNTHETIC BIFUNCTIONAL ENZYMEcoord: 113..315
score: 1.5

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmoCh04G019860Csa5G262270Cucumber (Chinese Long) v2cmocuB732
CmoCh04G019860CsaV3_5G012690Cucumber (Chinese Long) v3cmocucB0884
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmoCh04G019860Wax gourdcmowgoB0906
CmoCh04G019860Wax gourdcmowgoB0914
CmoCh04G019860Cucurbita maxima (Rimu)cmacmoB728
CmoCh04G019860Cucurbita pepo (Zucchini)cmocpeB673
CmoCh04G019860Bottle gourd (USVL1VR-Ls)cmolsiB623
CmoCh04G019860Silver-seed gourdcarcmoB0317