CsaV3_5G012690 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_5G012690
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	Thiamine phosphate synthase
Location	chr5 : 8187482 .. 8188011 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCGTGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTAATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGGTGTGGTGGAGTTTATCCCACTAACACAAAAGCGAACAATTTAACCGTTGGGATTGATGGATTGAAAAGAGTTTGCTTAGCTTCCAAATTGCCAGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGA

mRNA sequence

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCGTGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTAATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGA

Coding sequence (CDS)

ATGAACAGAGAAAAAGATGCTAAAACTCGTGATTTCTTGGAAGTAGCAAAGTCATGCATAAAGATTTGTCGTGCACATGGAGTTCCATTGTTGATCAACGATCGTATTGACATTGCACTTGCGTGTAATGCGGATGGTGTACACGTTGGTCAGTCCGATATTCCTGCTCATGAAGTTCGCCGCCTTCTTGGCCCAACAAGATCATCGGTGTCTCATGCAAGACAACGGAGCAAGCGGAACAGGCATGGATTGATGGTGCAGATTACATTGGTGGTAGCAATTGGCGGTATCAATCACACTAATGCAGCTGCTGTGATGGGAATTGGTATCCCAAATCTTAAAGGTGTTGCTGTTGTGTCAGCTCTTTTTGATAGGCAATGTGTTTTAGAGGAGGCCTCAAAGTTACATGCTACTTTAGTGGAGGCTACAACATGA

Protein sequence

MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRLLGPTRSSVSHARQRSKRNRHGLMVQITLVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT

Homology

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BLAST of CsaV3_5G012690 vs. NCBI nr
Match: KGN50789.1 (hypothetical protein Csa_5G262270 [Cucumis sativus])

HSP 1 Score: 216.9 bits (551), Expect = 4.7e-53
Identity = 123/176 (69.89%), Postives = 128/176 (72.73%), Query Frame = 0


Query: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQI 120
           RLLGP +       +   A Q                  +K N         +   +   
Sbjct: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 TLVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
             VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 176

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BLAST of CsaV3_5G012690 vs. NCBI nr
Match: XP_004140412.1 (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis sativus])

HSP 1 Score: 212.6 bits (540), Expect = 8.8e-52
Identity = 121/174 (69.54%), Postives = 126/174 (72.41%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       +   A Q                  +K N         +   +     
Sbjct: 419 LGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
           VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 532

[Close]

BLAST of CsaV3_5G012690 vs. NCBI nr
Match: XP_008460243.1 (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-49
Identity = 117/174 (67.24%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       ++  A Q                  +K N         +   +     
Sbjct: 419 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 532

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BLAST of CsaV3_5G012690 vs. NCBI nr
Match: XP_008460244.1 (PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-49
Identity = 117/174 (67.24%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 339 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 398

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       ++  A Q                  +K N         +   +     
Sbjct: 399 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 459 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 512

[Close]

BLAST of CsaV3_5G012690 vs. NCBI nr
Match: XP_022957338.1 (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 183.3 bits (464), Expect = 5.7e-43
Identity = 104/173 (60.12%), Postives = 114/173 (65.90%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLE AKSCI+IC  HGVPLLINDRID+ALAC ADGVH+GQSDIP H  R L
Sbjct: 339 REKDAKTRDFLEAAKSCIEICHTHGVPLLINDRIDVALACGADGVHIGQSDIPVHAARSL 398

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       +   A Q                  +K N         +   +     
Sbjct: 399 LGPDKIIGVSCKTPEQAEQAWLDGADYIGCGGVYPTNTKANNLTVGLDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 144
           VVAIGGINH+NAAAVM IG+PNLKGVAVVSALFDRQCVLEE  KLHATLVEAT
Sbjct: 459 VVAIGGINHSNAAAVMEIGVPNLKGVAVVSALFDRQCVLEETLKLHATLVEAT 511

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BLAST of CsaV3_5G012690 vs. TAIR10
Match: AT1G22940.1 (thiamin biosynthesis protein, putative)

HSP 1 Score: 146.4 bits (368), Expect = 1.4e-35
Identity = 88/172 (51.16%), Postives = 103/172 (59.88%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AK+CI ICR+HGV LLINDRIDIALAC+ADGVHVGQSD+P   VR L
Sbjct: 347 REKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSL 406

Query: 63  LGPTR-------------------------SSVSHARQRSKRNRHGL-------MVQITL 122
           LGP +                           V     ++     GL             
Sbjct: 407 LGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 143
           VVAIGGI  +NA +VM I  PNLKGVAVVSALFD+ CVL +A KLH TL E+
Sbjct: 467 VVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLHKTLKES 518

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BLAST of CsaV3_5G012690 vs. Swiss-Prot
Match: sp|Q5M731|TPS1L_ARATH (Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TH1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.5e-34
Identity = 88/172 (51.16%), Postives = 103/172 (59.88%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AK+CI ICR+HGV LLINDRIDIALAC+ADGVHVGQSD+P   VR L
Sbjct: 347 REKEAETREFLEEAKACIDICRSHGVSLLINDRIDIALACDADGVHVGQSDMPVDLVRSL 406

Query: 63  LGPTR-------------------------SSVSHARQRSKRNRHGL-------MVQITL 122
           LGP +                           V     ++     GL             
Sbjct: 407 LGPDKIIGVSCKTPEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLKEVCEASKLP 466

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 143
           VVAIGGI  +NA +VM I  PNLKGVAVVSALFD+ CVL +A KLH TL E+
Sbjct: 467 VVAIGGIGISNAGSVMQIDAPNLKGVAVVSALFDQDCVLTQAKKLHKTLKES 518

[Close]

BLAST of CsaV3_5G012690 vs. Swiss-Prot
Match: sp|Q2QWK9|TPS1_ORYSJ (Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0192500 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-33
Identity = 84/173 (48.55%), Postives = 108/173 (62.43%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKD++TR+FLE AK+C++IC++ GVPLLINDR+DIALACNADGVHVGQ D+ AHEVR L
Sbjct: 374 REKDSETREFLEAAKACMEICKSSGVPLLINDRVDIALACNADGVHVGQLDMSAHEVREL 433

Query: 63  LGPTR------SSVSHARQ-----------------RSKRNRHGL---------MVQITL 122
           LGP +       + + A+Q                  +K N   L         +     
Sbjct: 434 LGPGKIIGVSCKTPAQAQQAWNDGADYIGCGGVFPTSTKANNPTLGFDGLKTVCLASKLP 493

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 144
           VVAIGGIN +NA +VM +G+PNLKGVAVVSALFDR  V+ E   + + L   +
Sbjct: 494 VVAIGGINASNAGSVMELGLPNLKGVAVVSALFDRPSVVAETRNMKSILTNTS 546

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BLAST of CsaV3_5G012690 vs. Swiss-Prot
Match: sp|O48881|TPS1_BRANA (Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus OX=3708 GN=BTH1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 8.2e-33
Identity = 87/172 (50.58%), Postives = 105/172 (61.05%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REK+A+TR+FLE AKSC+ ICR++GV LLINDR DIA+A +ADGVHVGQSD+P   VR L
Sbjct: 348 REKEAETREFLEEAKSCVDICRSNGVCLLINDRFDIAIALDADGVHVGQSDMPVDLVRSL 407

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       +   A Q                  +K N         R         
Sbjct: 408 LGPDKIIGVSCKTQEQAHQAWKDGADYIGSGGVFPTNTKANNRTIGLDGLREVCKASKLP 467

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 143
           VVAIGGI  +NA +VM IG PNLKGVAVVSALFD++CVL +A KLH TL E+
Sbjct: 468 VVAIGGIGISNAESVMRIGEPNLKGVAVVSALFDQECVLTQAKKLHKTLTES 519

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BLAST of CsaV3_5G012690 vs. Swiss-Prot
Match: sp|A5UUL3|THIE_ROSS1 (Thiamine-phosphate synthase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=thiE PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-16
Identity = 61/173 (35.26%), Postives = 85/173 (49.13%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           R+KD   RD + + ++ + + R  GVPL++NDR+D+ALA +ADGVHVGQ DIPA  VRR+
Sbjct: 59  RDKDVPARDMVALGQALLPLTRDAGVPLIVNDRVDVALALDADGVHVGQDDIPAEMVRRI 118

Query: 63  LGPTR---SSVSHARQRSKRNRHGL-------------------------MVQITL---- 122
           +GP R    SV+   Q  +    G                          + +I      
Sbjct: 119 IGPERILGVSVATVEQAQRAMDAGATYVSVGDLFGTPSKPDAGPPIGLEPLAEIARTVNL 178

Query: 123 -VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEA 143
            V+ IGGIN  NAA+V+  G     GVAV+SA+            LHA +  A
Sbjct: 179 PVLGIGGINLANAASVIRAG---AVGVAVISAVIGAPDPEAATRALHAVIASA 228

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BLAST of CsaV3_5G012690 vs. Swiss-Prot
Match: sp|A8MK92|THIE_ALKOO (Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 GN=thiE PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.1e-16
Identity = 58/169 (34.32%), Postives = 91/169 (53.85%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR-- 62
           REK+A + +F ++A     +   + VPL+INDR+DIALA +ADGVHVGQSD+PAH VR  
Sbjct: 43  REKEASSLEFYQLALKAKALTEKYNVPLIINDRVDIALAVDADGVHVGQSDLPAHIVRSM 102

Query: 63  ----RLLGPTRSSVSHARQRSKRNRHGLMV-------------QITL------------- 122
               ++LG + +++  +++ ++     + V              +TL             
Sbjct: 103 IGQNKILGVSTATLEESKKAAEDGADYIGVGALFPTGTKTDANPVTLDQLRYIKENMDIP 162

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATL 140
           VV IGGI   N   +M +GI    GVA+VSA+  ++ + E A  L A++
Sbjct: 163 VVGIGGICEDNIKTIMEVGI---DGVAIVSAILGKENIKEAAESLKASI 208

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BLAST of CsaV3_5G012690 vs. TrEMBL
Match: tr|A0A0A0KPF9|A0A0A0KPF9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G262270 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.1e-53
Identity = 123/176 (69.89%), Postives = 128/176 (72.73%), Query Frame = 0


Query: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60
           MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR
Sbjct: 1   MNREKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVR 60

Query: 61  RLLGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQI 120
           RLLGP +       +   A Q                  +K N         +   +   
Sbjct: 61  RLLGPNKIIGVSCKTTEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASK 120

Query: 121 TLVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
             VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT
Sbjct: 121 LPVVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 176

[Close]

BLAST of CsaV3_5G012690 vs. TrEMBL
Match: tr|A0A1S3CCI6|A0A1S3CCI6_CUCME (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.1e-50
Identity = 117/174 (67.24%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 359 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 418

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       ++  A Q                  +K N         +   +     
Sbjct: 419 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 478

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 479 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 532

[Close]

BLAST of CsaV3_5G012690 vs. TrEMBL
Match: tr|A0A1S3CDB7|A0A1S3CDB7_CUCME (thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499122 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.1e-50
Identity = 117/174 (67.24%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKDAKTRDFLEVAKSCIKIC AHGVPLLINDRIDIALAC+ADGVHVGQSDIPAHEVRRL
Sbjct: 339 REKDAKTRDFLEVAKSCIKICHAHGVPLLINDRIDIALACDADGVHVGQSDIPAHEVRRL 398

Query: 63  LGPTR------SSVSHARQ-----------------RSKRN---------RHGLMVQITL 122
           LGP +       ++  A Q                  +K N         +   +     
Sbjct: 399 LGPNKVIGVSCKTMEQAEQAWIDGADYIGCGGVYPTNTKANNLTVGIDGLKRVCLASKLP 458

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
           VVAIGGINHTNAAAVMGIGIPNL+GVAVVSALFDRQCVLE ASKLHATLVEATT
Sbjct: 459 VVAIGGINHTNAAAVMGIGIPNLRGVAVVSALFDRQCVLEAASKLHATLVEATT 512

[Close]

BLAST of CsaV3_5G012690 vs. TrEMBL
Match: tr|A0A2P6PCZ4|A0A2P6PCZ4_ROSCH (Putative transferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0221201 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 6.2e-38
Identity = 95/173 (54.91%), Postives = 112/173 (64.74%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKD +TRDFLE AKSC++ICR+HGVPLLINDRID+ALAC+ADGVH+GQSD+P H  R L
Sbjct: 364 REKDIETRDFLEAAKSCLQICRSHGVPLLINDRIDVALACDADGVHIGQSDMPTHVARAL 423

Query: 63  LGPTR------SSVSHARQ-----------------RSKRNR-----HGL----MVQITL 122
           LGP +       +  HA Q                  +K N       GL    +     
Sbjct: 424 LGPEKIIGVSCKTPEHAEQAWIAGADYIGCGGVYPTNTKANNLTIGLEGLKTVCLASKLP 483

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEAT 144
           VVAIGGIN +NA +VM IG+PNLKGVAVVSALFDR+CVL E  KL   L+EAT
Sbjct: 484 VVAIGGINTSNAQSVMQIGVPNLKGVAVVSALFDRECVLTETKKLRTLLMEAT 536

[Close]

BLAST of CsaV3_5G012690 vs. TrEMBL
Match: tr|A0A2P5B8J3|A0A2P5B8J3_9ROSA (Thiamine phosphate synthase OS=Trema orientalis OX=63057 GN=TorRG33x02_329480 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 6.9e-37
Identity = 95/174 (54.60%), Postives = 111/174 (63.79%), Query Frame = 0


Query: 3   REKDAKTRDFLEVAKSCIKICRAHGVPLLINDRIDIALACNADGVHVGQSDIPAHEVRRL 62
           REKD +TRDFLE AKSC+KICR+HGVPLLINDR+D+ALAC+ADGVHVGQSD+PAH  R L
Sbjct: 379 REKDIETRDFLEAAKSCLKICRSHGVPLLINDRVDVALACDADGVHVGQSDMPAHIARTL 438

Query: 63  LGPTR------SSVSHARQ-----------------RSKRNR-----HGL----MVQITL 122
           LGP +       +   A Q                  +K N       GL    +     
Sbjct: 439 LGPEKIIGVSCKTPEQAEQAWFDGADYIGCGGVYPTNTKENNVTVGLDGLKTVCLASKLP 498

Query: 123 VVAIGGINHTNAAAVMGIGIPNLKGVAVVSALFDRQCVLEEASKLHATLVEATT 145
           VVAIGGI  +NA AVM I +P LKGVAVVSALFDRQCV  E  KLH+ L+EAT+
Sbjct: 499 VVAIGGIGLSNAHAVMEINVPQLKGVAVVSALFDRQCVSTETRKLHSVLIEATS 552

The following BLAST results are available for this feature:

BLAST of CsaV3_5G012690 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN50789.1	4.7e-53	69.89	hypothetical protein Csa_5G262270 [Cucumis sativus]	[more]
XP_004140412.1	8.8e-52	69.54	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic [Cucumis...	[more]
XP_008460243.1	1.1e-49	67.24	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
XP_008460244.1	1.1e-49	67.24	PREDICTED: thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform ...	[more]
XP_022957338.1	5.7e-43	60.12	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 [Cucurbi...	[more]

BLAST of CsaV3_5G012690 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G22940.1	1.4e-35	51.16	thiamin biosynthesis protein, putative	[more]

BLAST of CsaV3_5G012690 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q5M731\|TPS1L_ARATH	2.5e-34	51.16	Thiamine biosynthetic bifunctional enzyme TH1, chloroplastic OS=Arabidopsis thal...	[more]
sp\|Q2QWK9\|TPS1_ORYSJ	1.3e-33	48.55	Probable thiamine biosynthetic bifunctional enzyme, chloroplastic OS=Oryza sativ...	[more]
sp\|O48881\|TPS1_BRANA	8.2e-33	50.58	Thiamine biosynthetic bifunctional enzyme BTH1, chloroplastic OS=Brassica napus ...	[more]
sp\|A5UUL3\|THIE_ROSS1	2.4e-16	35.26	Thiamine-phosphate synthase OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=thiE P...	[more]
sp\|A8MK92\|THIE_ALKOO	3.1e-16	34.32	Thiamine-phosphate synthase OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688...	[more]

BLAST of CsaV3_5G012690 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KPF9\|A0A0A0KPF9_CUCSA	3.1e-53	69.89	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G262270 PE=3 SV=1	[more]
tr\|A0A1S3CCI6\|A0A1S3CCI6_CUCME	7.1e-50	67.24	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X2 OS=Cucum...	[more]
tr\|A0A1S3CDB7\|A0A1S3CDB7_CUCME	7.1e-50	67.24	thiamine biosynthetic bifunctional enzyme TH1, chloroplastic isoform X1 OS=Cucum...	[more]
tr\|A0A2P6PCZ4\|A0A2P6PCZ4_ROSCH	6.2e-38	54.91	Putative transferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr7g0221201 PE=3 SV...	[more]
tr\|A0A2P5B8J3\|A0A2P5B8J3_9ROSA	6.9e-37	54.60	Thiamine phosphate synthase OS=Trema orientalis OX=63057 GN=TorRG33x02_329480 PE...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036206	ThiamineP_synth_sf
IPR022998	ThiamineP_synth_TenI
IPR013785	Aldolase_TIM

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009228	thiamine biosynthetic process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0008902	hydroxymethylpyrimidine kinase activity
molecular_function	GO:0008972	phosphomethylpyrimidine kinase activity
molecular_function	GO:0004789	thiamine-phosphate diphosphorylase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_5G012690.1	CsaV3_5G012690.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 132..144
None	No IPR available	PANTHER	PTHR20857	THIAMINE-PHOSPHATE PYROPHOSPHORYLASE	coord: 91..139 coord: 3..66
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 84..142 e-value: 5.7E-7 score: 30.8
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 2..78 e-value: 7.2E-26 score: 92.6
IPR022998	Thiamine phosphate synthase/TenI	PFAM	PF02581	TMP-TENI	coord: 3..66 e-value: 9.4E-23 score: 80.4
IPR022998	Thiamine phosphate synthase/TenI	CDD	cd00564	TMP_TenI	coord: 3..137 e-value: 1.44942E-36 score: 124.938
IPR036206	Thiamin phosphate synthase superfamily	SUPERFAMILY	SSF51391	Thiamin phosphate synthase	coord: 2..136

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_5G012690	CSPI05G13000	Wild cucumber (PI 183967)	cpicucB299
CsaV3_5G012690	CmoCh04G019860	Cucurbita moschata (Rifu)	cmocucB0884
CsaV3_5G012690	MELO3C022605	Melon (DHL92) v3.5.1	cucmeB372
CsaV3_5G012690	MELO3C022605.2	Melon (DHL92) v3.6.1	cucmedB363
CsaV3_5G012690	Cla97C11G207240	Watermelon (97103) v2	cucwmbB375

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_5G012690	Wax gourd	cucwgoB521
CsaV3_5G012690	Silver-seed gourd	carcucB0773
CsaV3_5G012690	Cucumber (Gy14) v2	cgybcucB232
CsaV3_5G012690	Cucurbita maxima (Rimu)	cmacucB0876
CsaV3_5G012690	Cucurbita maxima (Rimu)	cmacucB0881
CsaV3_5G012690	Cucurbita moschata (Rifu)	cmocucB0862
CsaV3_5G012690	Cucurbita moschata (Rifu)	cmocucB0893
CsaV3_5G012690	Cucurbita pepo (Zucchini)	cpecucB0532
CsaV3_5G012690	Cucurbita pepo (Zucchini)	cpecucB0561
CsaV3_5G012690	Wild cucumber (PI 183967)	cpicucB285
CsaV3_5G012690	Wild cucumber (PI 183967)	cpicucB300
CsaV3_5G012690	Bottle gourd (USVL1VR-Ls)	cuclsiB363
CsaV3_5G012690	Melon (DHL92) v3.5.1	cucmeB379
CsaV3_5G012690	Melon (DHL92) v3.6.1	cucmedB371
CsaV3_5G012690	Watermelon (Charleston Gray)	cucwcgB377
CsaV3_5G012690	Watermelon (Charleston Gray)	cucwcgB379
CsaV3_5G012690	Watermelon (97103) v1	cucwmB463
CsaV3_5G012690	Watermelon (97103) v1	cucwmB465
CsaV3_5G012690	Watermelon (97103) v2	cucwmbB376