Lsi09G016700 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi09G016700
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Calcium-binding EF-hand family protein
Location	chr09 : 25113833 .. 25114207 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACCAAAATCATGATACCTCAAGCAAAGTAGATGAAGGATATGAAGACTTGTTGCCAGTGATGGCGCAAAAGCTCGACGTTGAGGTGTTTGTGGCTGAGCTTTGCAGCGGTTTTCGGCTGCTTGCCGACACAACGAAAGGGTTGATAACGGCAGAGAGTTTACGGCGAAACTCGGTGCTGTTGGGAATGGAAGGGATGAACGAGCACGAAGCAGAGTCGATGGTGAGGGAAGGAGATCTTGATGGGGATGGAGCTCTCAATGAAATGGAATTTTGTATTTTGATGGTGAGACTTAGTCCAGGAATGATGGAGGATGCTGAGGCTTGGCTTCAGAAGGCAATTGATGAAGAGCTAAGTAAATCTTCTTGTTAA

mRNA sequence

ATGGACCAAAATCATGATACCTCAAGCAAAGTAGATGAAGGATATGAAGACTTGTTGCCAGTGATGGCGCAAAAGCTCGACGTTGAGGTGTTTGTGGCTGAGCTTTGCAGCGGTTTTCGGCTGCTTGCCGACACAACGAAAGGGTTGATAACGGCAGAGAGTTTACGGCGAAACTCGGTGCTGTTGGGAATGGAAGGGATGAACGAGCACGAAGCAGAGTCGATGGTGAGGGAAGGAGATCTTGATGGGGATGGAGCTCTCAATGAAATGGAATTTTGTATTTTGATGGTGAGACTTAGTCCAGGAATGATGGAGGATGCTGAGGCTTGGCTTCAGAAGGCAATTGATGAAGAGCTAAGTAAATCTTCTTGTTAA

Coding sequence (CDS)

ATGGACCAAAATCATGATACCTCAAGCAAAGTAGATGAAGGATATGAAGACTTGTTGCCAGTGATGGCGCAAAAGCTCGACGTTGAGGTGTTTGTGGCTGAGCTTTGCAGCGGTTTTCGGCTGCTTGCCGACACAACGAAAGGGTTGATAACGGCAGAGAGTTTACGGCGAAACTCGGTGCTGTTGGGAATGGAAGGGATGAACGAGCACGAAGCAGAGTCGATGGTGAGGGAAGGAGATCTTGATGGGGATGGAGCTCTCAATGAAATGGAATTTTGTATTTTGATGGTGAGACTTAGTCCAGGAATGATGGAGGATGCTGAGGCTTGGCTTCAGAAGGCAATTGATGAAGAGCTAAGTAAATCTTCTTGTTAA

Protein sequence

MDQNHDTSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEELSKSSC

Homology

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BLAST of Lsi09G016700 vs. Swiss-Prot
Match: KIC_ARATH (Calcium-binding protein KIC OS=Arabidopsis thaliana GN=KIC PE=1 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 3.8e-39
Identity = 77/113 (68.14%), Postives = 98/113 (86.73%), Query Frame = 1


Query: 7   TSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEG 66
           +++K++  YED+LPVMA+K+DVE FV+ELC GF LLAD  + LITAESLRRNS +LG+EG
Sbjct: 14  STTKMETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEG 73

Query: 67  MNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
           M++ +A+ MVREGDLDGDGALN+ EFC+LMVRLSP MMEDAE WL+KA+ +EL
Sbjct: 74  MSKEDAQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQEL 126

[Close]

BLAST of Lsi09G016700 vs. Swiss-Prot
Match: PBP1_ARATH (Calcium-binding protein PBP1 OS=Arabidopsis thaliana GN=PBP1 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 9.3e-22
Identity = 52/105 (49.52%), Postives = 70/105 (66.67%), Query Frame = 1


Query: 15  YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRN-SVLLGMEGMNEHEAE 74
           ++D  P MA KL  E  + E+C GF LL D  KG+IT ESLRRN S +LG+  + + +  
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 75  SMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEE 119
            M+ EGD D DGALN+MEFC+LM RLSP +ME +   + + I+EE
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEE 123

[Close]

BLAST of Lsi09G016700 vs. Swiss-Prot
Match: CETN2_BOVIN (Centrin-2 OS=Bos taurus GN=CETN2 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 2.9e-07
Identity = 31/86 (36.05%), Postives = 49/86 (56.98%), Query Frame = 1


Query: 15  YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEGMNEHEAES 74
           + D L VM QK+  +    E+   F+L  D   G I+ ++L+R +  LG E +++ E + 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLSDEELQE 145

Query: 75  MVREGDLDGDGALNEMEFCILMVRLS 101
           M+ E D DGDG +NE EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of Lsi09G016700 vs. Swiss-Prot
Match: CETN1_HUMAN (Centrin-1 OS=Homo sapiens GN=CETN1 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.8e-07
Identity = 32/86 (37.21%), Postives = 48/86 (55.81%), Query Frame = 1


Query: 15  YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEGMNEHEAES 74
           + D L VM QK+  +    E+   FRL  D   G I+ ++L+R +  LG E + + E + 
Sbjct: 86  FNDFLAVMTQKMSEKDTKEEILKAFRLFDDDETGKISFKNLKRVANELG-ENLTDEELQE 145

Query: 75  MVREGDLDGDGALNEMEFCILMVRLS 101
           M+ E D DGDG +NE EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEEEFLRIMKKTS 170

[Close]

BLAST of Lsi09G016700 vs. Swiss-Prot
Match: CETN2_MOUSE (Centrin-2 OS=Mus musculus GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.8e-07
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 1


Query: 15  YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEGMNEHEAES 74
           + D L VM QK+  +    E+   F+L  D   G I+ ++L+R +  LG E + + E + 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQE 145

Query: 75  MVREGDLDGDGALNEMEFCILMVRLS 101
           M+ E D DGDG +NE EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of Lsi09G016700 vs. TrEMBL
Match: A0A0A0KAP2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G106800 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 3.6e-57
Identity = 117/125 (93.60%), Postives = 120/125 (96.00%), Query Frame = 1


Query: 1   MDQNHDTSSKV-DEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNS 60
           MDQNH+TS +V DEGYEDLLPVMAQKLDVEVFVAELCSGFRLLAD TKGLITAESLRRNS
Sbjct: 1   MDQNHNTSGRVLDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADATKGLITAESLRRNS 60

Query: 61  VLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
            LLGMEGMNE+EAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL
Sbjct: 61  ALLGMEGMNENEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120

Query: 121 SKSSC 125
            KSSC
Sbjct: 121 GKSSC 125

[Close]

BLAST of Lsi09G016700 vs. TrEMBL
Match: A0A061DS74_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_005098 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-43
Identity = 93/117 (79.49%), Postives = 100/117 (85.47%), Query Frame = 1


Query: 7   TSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEG 66
           T S     YEDLLPVMA+KLDVE FV+ELC GFRLLAD  +GLIT ESLRRNS LLGMEG
Sbjct: 9   TPSTTTSQYEDLLPVMAEKLDVEAFVSELCGGFRLLADQERGLITPESLRRNSALLGMEG 68

Query: 67  MNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEELSKSS 124
           M++ EAE MVREGDLDGDGALN+ EFCILMVRLSPGMMEDAE WLQKAID+EL KSS
Sbjct: 69  MSKDEAEGMVREGDLDGDGALNQTEFCILMVRLSPGMMEDAETWLQKAIDQELRKSS 125

[Close]

BLAST of Lsi09G016700 vs. TrEMBL
Match: A0A0B2PAH4_GLYSO (Calcium-binding protein KIC OS=Glycine soja GN=glysoja_021168 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 7.4e-42
Identity = 89/124 (71.77%), Postives = 106/124 (85.48%), Query Frame = 1


Query: 1   MDQNHDT-SSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNS 60
           M+ NH    + ++  +EDLLPVMA+KLDVE FV+ELC GF+LL+D   GLIT+ESLR NS
Sbjct: 1   MESNHSMRQNTMETEFEDLLPVMAEKLDVESFVSELCGGFKLLSDPETGLITSESLRTNS 60

Query: 61  VLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
            LLGMEGM++ +AE+MVR+GDLDGDG LNE EFCILMVRLSPGMMEDAE+WL+KAIDEEL
Sbjct: 61  ALLGMEGMSKEDAEAMVRQGDLDGDGKLNETEFCILMVRLSPGMMEDAESWLEKAIDEEL 120

Query: 121 SKSS 124
           SKSS
Sbjct: 121 SKSS 124

[Close]

BLAST of Lsi09G016700 vs. TrEMBL
Match: C6TM17_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_07G053300 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 7.4e-42
Identity = 89/124 (71.77%), Postives = 106/124 (85.48%), Query Frame = 1


Query: 1   MDQNHDT-SSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNS 60
           M+ NH    + ++  +EDLLPVMA+KLDVE FV+ELC GF+LL+D   GLIT+ESLR NS
Sbjct: 1   MESNHSMRQNTMETEFEDLLPVMAEKLDVESFVSELCGGFKLLSDPETGLITSESLRTNS 60

Query: 61  VLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
            LLGMEGM++ +AE+MVR+GDLDGDG LNE EFCILMVRLSPGMMEDAE+WL+KAIDEEL
Sbjct: 61  ALLGMEGMSKEDAEAMVRQGDLDGDGKLNETEFCILMVRLSPGMMEDAESWLEKAIDEEL 120

Query: 121 SKSS 124
           SKSS
Sbjct: 121 SKSS 124

[Close]

BLAST of Lsi09G016700 vs. TrEMBL
Match: B9SBD1_RICCO (Ccd1, putative OS=Ricinus communis GN=RCOM_0715990 PE=4 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 4.8e-41
Identity = 89/117 (76.07%), Postives = 98/117 (83.76%), Query Frame = 1


Query: 7   TSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEG 66
           T +     YEDLLPVMA+KLDV+ FV+ELC GFRLLAD  K LIT+ESLRRNS LLGMEG
Sbjct: 8   TKTATSRVYEDLLPVMAEKLDVDSFVSELCGGFRLLADPEKRLITSESLRRNSALLGMEG 67

Query: 67  MNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEELSKSS 124
           M++ ++E MVREGDLDGDGALNE EFCILMVRLSP MMEDAE WLQKAID EL KSS
Sbjct: 68  MSKEDSEEMVREGDLDGDGALNETEFCILMVRLSPEMMEDAEDWLQKAIDHELGKSS 124

[Close]

BLAST of Lsi09G016700 vs. TAIR10
Match: AT2G46600.1 (AT2G46600.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 162.2 bits (409), Expect = 2.1e-40
Identity = 77/113 (68.14%), Postives = 98/113 (86.73%), Query Frame = 1


Query: 7   TSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEG 66
           +++K++  YED+LPVMA+K+DVE FV+ELC GF LLAD  + LITAESLRRNS +LG+EG
Sbjct: 14  STTKMETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEG 73

Query: 67  MNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
           M++ +A+ MVREGDLDGDGALN+ EFC+LMVRLSP MMEDAE WL+KA+ +EL
Sbjct: 74  MSKEDAQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQEL 126

[Close]

BLAST of Lsi09G016700 vs. TAIR10
Match: AT4G27280.1 (AT4G27280.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 106.3 bits (264), Expect = 1.4e-23
Identity = 53/113 (46.90%), Postives = 72/113 (63.72%), Query Frame = 1


Query: 7   TSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSV-LLGME 66
           T       + D LP MA  L  E  + ELC+GF LL D  KG+IT ESLRRN+  +LG+ 
Sbjct: 11  TQQNPQPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLG 70

Query: 67  GMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEE 119
            + + +   M++EGD D DGALN+MEFC+LM RLSP +ME +   + + I+EE
Sbjct: 71  DLTDEDVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEE 123

[Close]

BLAST of Lsi09G016700 vs. TAIR10
Match: AT5G54490.1 (AT5G54490.1 pinoid-binding protein 1)

HSP 1 Score: 104.4 bits (259), Expect = 5.2e-23
Identity = 52/105 (49.52%), Postives = 70/105 (66.67%), Query Frame = 1


Query: 15  YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRN-SVLLGMEGMNEHEAE 74
           ++D  P MA KL  E  + E+C GF LL D  KG+IT ESLRRN S +LG+  + + +  
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 75  SMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEE 119
            M+ EGD D DGALN+MEFC+LM RLSP +ME +   + + I+EE
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEE 123

[Close]

BLAST of Lsi09G016700 vs. TAIR10
Match: AT4G14640.1 (AT4G14640.1 calmodulin 8)

HSP 1 Score: 52.4 bits (124), Expect = 2.4e-07
Identity = 35/111 (31.53%), Postives = 55/111 (49.55%), Query Frame = 1


Query: 1   MDQN------HDTSSKVDEG------YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKG 60
           +DQN      HD  +++D        + + L +MA+KL       EL   F++      G
Sbjct: 41  LDQNPTEQELHDIITEIDSDSNGTIEFAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNG 100

Query: 61  LITAESLRRNSVLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRL 100
            I+A  L    + LG E + + E E M++E DLDGDG +N  EF  +M+ +
Sbjct: 101 YISASELSHVMINLG-EKLTDEEVEQMIKEADLDGDGQVNYDEFVKMMINI 150

[Close]

BLAST of Lsi09G016700 vs. TAIR10
Match: AT3G51920.1 (AT3G51920.1 calmodulin 9)

HSP 1 Score: 51.2 bits (121), Expect = 5.3e-07
Identity = 34/86 (39.53%), Postives = 45/86 (52.33%), Query Frame = 1


Query: 15  YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEGMNEHEAES 74
           ++D L +MAQ    E    EL   FR+      GLI+   L      +GM+ +   EAE 
Sbjct: 66  FDDFLYIMAQNTSQESASDELIEVFRVFDRDGDGLISQLELGEGMKDMGMK-ITAEEAEH 125

Query: 75  MVREGDLDGDGALNEMEFCILMVRLS 101
           MVRE DLDGDG L+  EF  +M+  S
Sbjct: 126 MVREADLDGDGFLSFHEFSKMMIAAS 150

[Close]

BLAST of Lsi09G016700 vs. NCBI nr
Match: gi|449445489|ref|XP_004140505.1| (PREDICTED: calcium-binding protein KIC [Cucumis sativus])

HSP 1 Score: 228.8 bits (582), Expect = 5.2e-57
Identity = 117/125 (93.60%), Postives = 120/125 (96.00%), Query Frame = 1


Query: 1   MDQNHDTSSKV-DEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNS 60
           MDQNH+TS +V DEGYEDLLPVMAQKLDVEVFVAELCSGFRLLAD TKGLITAESLRRNS
Sbjct: 1   MDQNHNTSGRVLDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADATKGLITAESLRRNS 60

Query: 61  VLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
            LLGMEGMNE+EAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL
Sbjct: 61  ALLGMEGMNENEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120

Query: 121 SKSSC 125
            KSSC
Sbjct: 121 GKSSC 125

[Close]

BLAST of Lsi09G016700 vs. NCBI nr
Match: gi|590721005|ref|XP_007051487.1| (Calcium-binding EF-hand family protein [Theobroma cacao])

HSP 1 Score: 184.1 bits (466), Expect = 1.5e-43
Identity = 93/117 (79.49%), Postives = 100/117 (85.47%), Query Frame = 1


Query: 7   TSSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNSVLLGMEG 66
           T S     YEDLLPVMA+KLDVE FV+ELC GFRLLAD  +GLIT ESLRRNS LLGMEG
Sbjct: 9   TPSTTTSQYEDLLPVMAEKLDVEAFVSELCGGFRLLADQERGLITPESLRRNSALLGMEG 68

Query: 67  MNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEELSKSS 124
           M++ EAE MVREGDLDGDGALN+ EFCILMVRLSPGMMEDAE WLQKAID+EL KSS
Sbjct: 69  MSKDEAEGMVREGDLDGDGALNQTEFCILMVRLSPGMMEDAETWLQKAIDQELRKSS 125

[Close]

BLAST of Lsi09G016700 vs. NCBI nr
Match: gi|1009142463|ref|XP_015888736.1| (PREDICTED: calcium-binding protein KIC-like [Ziziphus jujuba])

HSP 1 Score: 178.3 bits (451), Expect = 8.1e-42
Identity = 90/125 (72.00%), Postives = 106/125 (84.80%), Query Frame = 1


Query: 1   MDQNHDTSSKVDEG--YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRN 60
           M+QN     K+     YEDLLPVMA+KLDV+ F++ELC GF+LLA+  +GLITA SLR+N
Sbjct: 5   MEQNQTGGGKISTSTEYEDLLPVMAEKLDVDNFMSELCKGFQLLAEPGRGLITAGSLRKN 64

Query: 61  SVLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEE 120
           S LLGMEGM++ +AE+MVREGDLDGDGALNE EFCILMVRLSPGMMEDAEAWL+KAID+E
Sbjct: 65  SALLGMEGMSKEDAEAMVREGDLDGDGALNETEFCILMVRLSPGMMEDAEAWLEKAIDQE 124

Query: 121 LSKSS 124
           L KSS
Sbjct: 125 LKKSS 129

[Close]

BLAST of Lsi09G016700 vs. NCBI nr
Match: gi|358248524|ref|NP_001239896.1| (uncharacterized protein LOC100801586 [Glycine max])

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-41
Identity = 89/124 (71.77%), Postives = 106/124 (85.48%), Query Frame = 1


Query: 1   MDQNHDT-SSKVDEGYEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRNS 60
           M+ NH    + ++  +EDLLPVMA+KLDVE FV+ELC GF+LL+D   GLIT+ESLR NS
Sbjct: 1   MESNHSMRQNTMETEFEDLLPVMAEKLDVESFVSELCGGFKLLSDPETGLITSESLRTNS 60

Query: 61  VLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEEL 120
            LLGMEGM++ +AE+MVR+GDLDGDG LNE EFCILMVRLSPGMMEDAE+WL+KAIDEEL
Sbjct: 61  ALLGMEGMSKEDAEAMVRQGDLDGDGKLNETEFCILMVRLSPGMMEDAESWLEKAIDEEL 120

Query: 121 SKSS 124
           SKSS
Sbjct: 121 SKSS 124

[Close]

BLAST of Lsi09G016700 vs. NCBI nr
Match: gi|694412072|ref|XP_009334371.1| (PREDICTED: calcium-binding protein KIC [Pyrus x bretschneideri])

HSP 1 Score: 176.4 bits (446), Expect = 3.1e-41
Identity = 89/125 (71.20%), Postives = 105/125 (84.00%), Query Frame = 1


Query: 1   MDQNHDTSSKVDEG--YEDLLPVMAQKLDVEVFVAELCSGFRLLADTTKGLITAESLRRN 60
           M+    TS K  E   Y+DLLPVMA+KLDVE FV+ELC GFRLLAD  +GLIT+ESL++N
Sbjct: 1   MENKGTTSRKSTEPTEYQDLLPVMAEKLDVETFVSELCGGFRLLADPERGLITSESLKKN 60

Query: 61  SVLLGMEGMNEHEAESMVREGDLDGDGALNEMEFCILMVRLSPGMMEDAEAWLQKAIDEE 120
           S LLGMEGM++ +AE MVREGDLDGDGAL E EFCILMVRLSPG+MEDAE WL+KA+D+E
Sbjct: 61  SALLGMEGMSKEDAEGMVREGDLDGDGALGETEFCILMVRLSPGIMEDAETWLEKALDQE 120

Query: 121 LSKSS 124
           L+KSS
Sbjct: 121 LNKSS 125

The following BLAST results are available for this feature:

BLAST of Lsi09G016700 vs. Swiss-Prot


Match Name	E-value	Identity	Description
KIC_ARATH	3.8e-39	68.14	Calcium-binding protein KIC OS=Arabidopsis thaliana GN=KIC PE=1 SV=2	[more]
PBP1_ARATH	9.3e-22	49.52	Calcium-binding protein PBP1 OS=Arabidopsis thaliana GN=PBP1 PE=1 SV=1	[more]
CETN2_BOVIN	2.9e-07	36.05	Centrin-2 OS=Bos taurus GN=CETN2 PE=2 SV=1	[more]
CETN1_HUMAN	3.8e-07	37.21	Centrin-1 OS=Homo sapiens GN=CETN1 PE=1 SV=1	[more]
CETN2_MOUSE	3.8e-07	36.05	Centrin-2 OS=Mus musculus GN=Cetn2 PE=1 SV=1	[more]

BLAST of Lsi09G016700 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAP2_CUCSA	3.6e-57	93.60	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G106800 PE=4 SV=1	[more]
A0A061DS74_THECC	1.0e-43	79.49	Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_005098 PE=4 SV=...	[more]
A0A0B2PAH4_GLYSO	7.4e-42	71.77	Calcium-binding protein KIC OS=Glycine soja GN=glysoja_021168 PE=4 SV=1	[more]
C6TM17_SOYBN	7.4e-42	71.77	Uncharacterized protein OS=Glycine max GN=GLYMA_07G053300 PE=2 SV=1	[more]
B9SBD1_RICCO	4.8e-41	76.07	Ccd1, putative OS=Ricinus communis GN=RCOM_0715990 PE=4 SV=1	[more]

BLAST of Lsi09G016700 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G46600.1	2.1e-40	68.14	Calcium-binding EF-hand family protein	[more]
AT4G27280.1	1.4e-23	46.90	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	5.2e-23	49.52	pinoid-binding protein 1	[more]
AT4G14640.1	2.4e-07	31.53	calmodulin 8	[more]
AT3G51920.1	5.3e-07	39.53	calmodulin 9	[more]

BLAST of Lsi09G016700 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449445489\|ref\|XP_004140505.1\|	5.2e-57	93.60	PREDICTED: calcium-binding protein KIC [Cucumis sativus]	[more]
gi\|590721005\|ref\|XP_007051487.1\|	1.5e-43	79.49	Calcium-binding EF-hand family protein [Theobroma cacao]	[more]
gi\|1009142463\|ref\|XP_015888736.1\|	8.1e-42	72.00	PREDICTED: calcium-binding protein KIC-like [Ziziphus jujuba]	[more]
gi\|358248524\|ref\|NP_001239896.1\|	1.1e-41	71.77	uncharacterized protein LOC100801586 [Glycine max]	[more]
gi\|694412072\|ref\|XP_009334371.1\|	3.1e-41	71.20	PREDICTED: calcium-binding protein KIC [Pyrus x bretschneideri]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010091	trichome branching
biological_process	GO:0042744	hydrogen peroxide catabolic process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi09G016700.1	Lsi09G016700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 47..98 score: 4.2
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 67..102 score: 9
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 16..98 score: 2.8
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 19..119 score: 4.56
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 80..92 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..122 score: 6.8
None	No IPR available	PANTHER	PTHR10891:SF624	CALCIUM-BINDING PROTEIN KIC	coord: 15..122 score: 6.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi09G016700	Csa6G106800	Cucumber (Chinese Long) v2	culsiB402
Lsi09G016700	CSPI06G08440	Wild cucumber (PI 183967)	cpilsiB404
Lsi09G016700	Cucsa.120930	Cucumber (Gy14) v1	cgylsiB169
Lsi09G016700	CmoCh17G004820	Cucurbita moschata (Rifu)	cmolsiB320
Lsi09G016700	Cla006701	Watermelon (97103) v1	lsiwmB036
Lsi09G016700	MELO3C022259	Melon (DHL92) v3.5.1	lsimeB017
Lsi09G016700	ClCG06G002680	Watermelon (Charleston Gray)	lsiwcgB029
Lsi09G016700	MELO3C022259.2	Melon (DHL92) v3.6.1	lsimedB019
Lsi09G016700	Cla97C06G112040	Watermelon (97103) v2	lsiwmbB026
Lsi09G016700	Bhi12G001958	Wax gourd	lsiwgoB011
Lsi09G016700	Cp4.1LG12g04330	Cucurbita pepo (Zucchini)	cpelsiB101
Lsi09G016700	CsGy6G008520	Cucumber (Gy14) v2	cgyblsiB366
Lsi09G016700	Carg26681	Silver-seed gourd	carlsiB820
Lsi09G016700	CsaV3_6G008810	Cucumber (Chinese Long) v3	cuclsiB424

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi09G016700	Cucurbita maxima (Rimu)	cmalsiB331
Lsi09G016700	Cucurbita maxima (Rimu)	cmalsiB814
Lsi09G016700	Cucurbita moschata (Rifu)	cmolsiB800
Lsi09G016700	Cucurbita pepo (Zucchini)	cpelsiB251
Lsi09G016700	Melon (DHL92) v3.5.1	lsimeB018
Lsi09G016700	Watermelon (Charleston Gray)	lsiwcgB026
Lsi09G016700	Watermelon (97103) v1	lsiwmB042
Lsi09G016700	Silver-seed gourd	carlsiB070