Lsi08G009450 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi08G009450
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionEukaryotic translation initiation factor, putative
Locationchr08 : 17924831 .. 17926177 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTGCAGAATATTGGTGCCTCAAACCGTGATGATGCCTTCTATAGGTATAAGATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAAGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTCGGATGTGAGCTTGGTGCGCAATCCAAGTTCGATGAGAAAACCGGGACTTCTCTTGTAAATGGTGCTCATGAGACAGCAAAGCTGGCTGGTCTTCTCGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGAAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACGTTTATTCTCAAGAATCCTCCCGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGACTTAAGGAGGGTGAGGCTGCTGACGAGGAGCAGAAGAAAATAAAGAAGGATTCGCTCAAGAAGAAAAGCACAAGTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAGGATCATTCTCCAACTCGTAGCCATGCTGGTGATAATAACCAAGCAGCTGATGACGATGACGACGACGATGTGCAGTGGCAAACAGACACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGCGCTGTCACTGCTGATATGGTCATGCTCTCTACTACTGAAGAGAACAAGTCGTTAAAGAAGTCCCCTCAGCACCCTGAGCACGAGAAGTCTGCAGTCAATGGAAACAAGAATGGGAATGGAGTTTCTACCCATGGTGCCCTCGTTCAAGAGATCAAAGATTATTTGAAGAAAGGTTCCTCCGCAGCTGATCTCAAATCATTCTTGGAATCACTTTTCGGAACTCGCCAAGAGATTGTGAATGCCTTGGTTGAGGCTTTATTCGATGGTGTCGGTAAAGGCTTCTCAAAGGAGGTAATGAAAAAGAAGAAGTTCCTGGCTGCAGCTGCCGCTCAGGAAGGATCACAGATAATTCTTTTGCGCGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCAGGTAAGGAGGTTGCTCTAGTCCTGAAATCGTTATACGACAGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAACAGGGTGTGGCTGGTGCTAACAAAAGCTCTCAAATCTGGAAGTATGTAAAGCCCTTCATCGAATGGCTCCAGAATGCCGAGTCGGAGACAGAAGACGAGTAA

mRNA sequence

ATGGCGTTGCAGAATATTGGTGCCTCAAACCGTGATGATGCCTTCTATAGGTATAAGATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAAGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTCGGATGTGAGCTTGGTGCGCAATCCAAGTTCGATGAGAAAACCGGGACTTCTCTTGTAAATGGTGCTCATGAGACAGCAAAGCTGGCTGGTCTTCTCGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGAAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACGTTTATTCTCAAGAATCCTCCCGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGACTTAAGGAGGGTGAGGCTGCTGACGAGGAGCAGAAGAAAATAAAGAAGGATTCGCTCAAGAAGAAAAGCACAAGTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAGGATCATTCTCCAACTCGTAGCCATGCTGGTGATAATAACCAAGCAGCTGATGACGATGACGACGACGATGTGCAGTGGCAAACAGACACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGCGCTGTCACTGCTGATATGGTCATGCTCTCTACTACTGAAGAGAACAAGTCGTTAAAGAAGTCCCCTCAGCACCCTGAGCACGAGAAGTCTGCAGTCAATGGAAACAAGAATGGGAATGGAGTTTCTACCCATGGTGCCCTCGTTCAAGAGATCAAAGATTATTTGAAGAAAGGTTCCTCCGCAGCTGATCTCAAATCATTCTTGGAATCACTTTTCGGAACTCGCCAAGAGATTGTGAATGCCTTGGTTGAGGCTTTATTCGATGGTGTCGGTAAAGGCTTCTCAAAGGAGGTAATGAAAAAGAAGAAGTTCCTGGCTGCAGCTGCCGCTCAGGAAGGATCACAGATAATTCTTTTGCGCGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCAGGTAAGGAGGTTGCTCTAGTCCTGAAATCGTTATACGACAGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAACAGGGTGTGGCTGGTGCTAACAAAAGCTCTCAAATCTGGAAGTATGTAAAGCCCTTCATCGAATGGCTCCAGAATGCCGAGTCGGAGACAGAAGACGAGTAA

Coding sequence (CDS)

ATGGCGTTGCAGAATATTGGTGCCTCAAACCGTGATGATGCCTTCTATAGGTATAAGATGCCTAAGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATCAAGACTAATGTAGTTAACATGGTGGAAATTGCAAAAGCCTTGGGCAGACCTGCCTCTTACACTACCAAGTACTTCGGATGTGAGCTTGGTGCGCAATCCAAGTTCGATGAGAAAACCGGGACTTCTCTTGTAAATGGTGCTCATGAGACAGCAAAGCTGGCTGGTCTTCTCGAAAACTTCATCAAGAAATATGTTCAATGCTATGGATGTGGAAACCCTGAGACTGAGATAATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTCATATCTGATGTTGACATGAGAGATAAACTCACAACGTTTATTCTCAAGAATCCTCCCGAACAGAAGAAGGGATCAAAAGACAAGAAAGCAATGAGGAGGGCTGAGAAGGAACGACTTAAGGAGGGTGAGGCTGCTGACGAGGAGCAGAAGAAAATAAAGAAGGATTCGCTCAAGAAGAAAAGCACAAGTACTACAAAGGATGCTGTAACCAAAGTTCCAAGCAAGAAAAAGAATAATGGCTCAGATGAGGATCATTCTCCAACTCGTAGCCATGCTGGTGATAATAACCAAGCAGCTGATGACGATGACGACGACGATGTGCAGTGGCAAACAGACACTTCTTTAGAGGCAGCCAAGCAGCGTATTCAGGAGCAGTTGAGCGCTGTCACTGCTGATATGGTCATGCTCTCTACTACTGAAGAGAACAAGTCGTTAAAGAAGTCCCCTCAGCACCCTGAGCACGAGAAGTCTGCAGTCAATGGAAACAAGAATGGGAATGGAGTTTCTACCCATGGTGCCCTCGTTCAAGAGATCAAAGATTATTTGAAGAAAGGTTCCTCCGCAGCTGATCTCAAATCATTCTTGGAATCACTTTTCGGAACTCGCCAAGAGATTGTGAATGCCTTGGTTGAGGCTTTATTCGATGGTGTCGGTAAAGGCTTCTCAAAGGAGGTAATGAAAAAGAAGAAGTTCCTGGCTGCAGCTGCCGCTCAGGAAGGATCACAGATAATTCTTTTGCGCGCCATCGAATCTTTCTGTCTCAACACAAGTCCAGAGGCAGGTAAGGAGGTTGCTCTAGTCCTGAAATCGTTATACGACAGCGATGTAATAGAGGAGGAATTCGTACTAGAGTGGTACCAACAGGGTGTGGCTGGTGCTAACAAAAGCTCTCAAATCTGGAAGTATGTAAAGCCCTTCATCGAATGGCTCCAGAATGCCGAGTCGGAGACAGAAGACGAGTAA

Protein sequence

MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVSTHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKKKFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEDE
Homology
[Close]
BLAST of Lsi08G009450 vs. Swiss-Prot
Match: IF5_PHAVU (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris GN=EIF5 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.0e-159
Identity = 310/452 (68.58%), Postives = 363/452 (80.31%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGA N DDAFYRYKMP+MVTKIEGRGNGIKTNVVNMV+IAK L RPASYTTKYFG
Sbjct: 1   MALQNIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTS VNGAHETAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI 
Sbjct: 61  CELGAQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKV 180

Query: 181 KKDSLKKKSTSTTKDAVTK-VPSKKKNNGSDED-HSPTRSHAGDNNQAADDDDDDD--VQ 240
           KK+ +KKK +S+ KD   K   SKKK +GSDED  SPT     +  +A D+DDDDD  VQ
Sbjct: 181 KKE-VKKKGSSSAKDGTAKSTISKKKGSGSDEDRRSPTHKQIEEKEEAKDEDDDDDDGVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGV 300
           W TDTSL+A++QRI+EQLSAVTADMVML+T E  K  K +        +   G++NGN  
Sbjct: 241 WLTDTSLDASRQRIKEQLSAVTADMVMLTTDEPEKKKKAA-------SNQNGGSQNGNS- 300

Query: 301 STHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKK 360
             +G +V E+K  LKKG  A++L S L +L    QE ++ALVEALF+G  KGF +E +KK
Sbjct: 301 KNYGTVVAEVKANLKKGFGASELLSHLAALPVPAQEKMSALVEALFEGTEKGFGRETLKK 360

Query: 361 KKFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQ 420
           K + AAA A+EGSQI+LL AIE FC   +  A KEVALVLK+LYD+DV+EEE ++ WYQ+
Sbjct: 361 KNYFAAAVAEEGSQILLLHAIEEFCCKPNSNALKEVALVLKTLYDADVLEEEAIVLWYQK 420

Query: 421 GVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           G+ G NK+S+IWK  +PFI+WLQNAESE+++E
Sbjct: 421 GLKGDNKNSKIWKNAQPFIDWLQNAESESDEE 443

[Close]
BLAST of Lsi08G009450 vs. Swiss-Prot
Match: IF5_MAIZE (Eukaryotic translation initiation factor 5 OS=Zea mays GN=EIF5 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.0e-155
Identity = 308/460 (66.96%), Postives = 361/460 (78.48%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG SLVNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI+
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMIS 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKN--NGSDEDH--SPTRSHAGDNNQAADDDDDDDVQ 240
           KKD+ KK S    KD+  K   KK     GSDEDH  SPTRSH GD  +AA DDDDDDVQ
Sbjct: 181 KKDAKKKGS----KDSTAKGLKKKATTATGSDEDHSSSPTRSHDGD--KAAADDDDDDVQ 240

Query: 241 WQTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNG--- 300
           WQTDTS+EAAKQR+QEQLSA TA+MVMLST E  K +K+    P H+  + NG+      
Sbjct: 241 WQTDTSIEAAKQRMQEQLSAATAEMVMLSTEETEKKMKQ----PTHKDGSTNGSAKEIPN 300

Query: 301 -----NGVSTHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKG 360
                   S +  L+ +IK  L    + + LK+ L S     Q+++NA +EALF GVGKG
Sbjct: 301 DKPAVTKPSPYEELIGDIKASLGSAPTPSQLKAVLASSTLPPQDVMNAPLEALFGGVGKG 360

Query: 361 FSKEVMKKKKFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEE 420
           F+KEV+K KK+LA A   EG+Q +L++AIE+F    +PEA KEV +VLK+LYD D+++EE
Sbjct: 361 FTKEVVKNKKYLAVAVPDEGAQTLLVQAIEAFGGKCNPEALKEVPVVLKALYDGDILDEE 420

Query: 421 FVLEWYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
            +++WY   VA A K SQ+ K  KPF+EWLQ+AES+ E +
Sbjct: 421 TIVDWYNDAVA-AGKDSQVVKNAKPFVEWLQSAESDEEGD 449

[Close]
BLAST of Lsi08G009450 vs. Swiss-Prot
Match: IF5Y_ARATH (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 3.0e-155
Identity = 302/456 (66.23%), Postives = 357/456 (78.29%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTD 240
           KK++  KK  +TT  +  KV  KK       DHSP RS + +N+QA  ++DDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNG---VS 300
           TS EAA++R++EQLSAVTA+MVMLST EE K + +  + PE        ++NGN     +
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSKIPEN 300

Query: 301 THGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKK 360
            H  LV EIK+ L  GSS   LK+ L S     QE ++AL  ALF G GKGF+KEV+KKK
Sbjct: 301 AHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKK 360

Query: 361 KFLAA-----AAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLE 420
           K+L A       A   +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ ++E
Sbjct: 361 KYLLALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVE 420

Query: 421 WYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           WY +GV    KSS + K V PFIEWLQNAESE+E+E
Sbjct: 421 WYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]
BLAST of Lsi08G009450 vs. Swiss-Prot
Match: IF5Z_ARATH (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana GN=At1g77840 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.0e-154
Identity = 310/456 (67.98%), Postives = 361/456 (79.17%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQA---ADDDDDDDVQW 240
           K    KKK+ S  KD+ T      KN+ SDED SP      D N      D+DDDD V+W
Sbjct: 181 K---AKKKALSNGKDSKT-----SKNHSSDEDISPKH----DENALEVDEDEDDDDGVEW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGN--KNGNG 300
           QTDTS EAA++R+ EQLSA TA+MVMLS  E  +  KK+P      KS  NGN  K  N 
Sbjct: 241 QTDTSREAAEKRMMEQLSAKTAEMVMLSAMEVEE--KKAP------KSKSNGNVVKTENP 300

Query: 301 VSTHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMK 360
                 LVQ++K+YLKKGS  + LKSF+ SL    Q+I++AL  ALFDGVGKGF+KEV K
Sbjct: 301 PPQEKNLVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTK 360

Query: 361 KKKFLAAAAA--QEGSQIILLRAIESFC-LNTSPEAGKEVALVLKSLYDSDVIEEEFVLE 420
           KK +LAAAA   ++GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL+
Sbjct: 361 KKNYLAAAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLD 420

Query: 421 WYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           WY++G+ GA+KSS +WK VKPF+EWLQ+AESE+E+E
Sbjct: 421 WYEKGLTGADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]
BLAST of Lsi08G009450 vs. Swiss-Prot
Match: IF5_RAT (Eukaryotic translation initiation factor 5 OS=Rattus norvegicus GN=Eif5 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.6e-47
Identity = 142/444 (31.98%), Postives = 219/444 (49.32%), Query Frame = 1 

Query: 13  DAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEK 72
           D FYRYKMP+++ K+EG+GNGIKT +VNMV++AKAL RP +Y TKYFGCELGAQ++FD K
Sbjct: 11  DQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVK 70

Query: 73  TGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMITMKCAACGFISD 132
               +VNG+HE  KL  +L+ FIKK+V C  C NPET++ +  K Q I   C ACG+   
Sbjct: 71  NDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGM 130

Query: 133 VDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKIKKDSLKKKSTS 192
           +D   KL TFILKNPPE       KK                 E++KK +K   K+  + 
Sbjct: 131 LDTHHKLCTFILKNPPENSDIGTGKK-----------------EKEKKNRKGKDKENGSV 190

Query: 193 TTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTDTSLEAAKQRIQ 252
           +T +     P          + SP          A ++++DDD  W  DT+ EA ++R+ 
Sbjct: 191 STSETPPPPPPN--------EISPPH--------AVEEEEDDD--WGEDTTEEAQRRRMD 250

Query: 253 EQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVSTHGALVQEIKDYLK 312
           E           +S   +  +L    +    E+                  V  + D++K
Sbjct: 251 E-----------ISDHAKGLTLSDDLERTVEER------------------VNILFDFVK 310

Query: 313 KGSSAADLKSFLESLFGTRQEI-VNA-----LVEALFDGVGKGFSKEVMK-KKKFLAAAA 372
           K      + S  + +    + + V A     L E LFD   +   +++ K ++ FL    
Sbjct: 311 KKKEEGIIDSSDKDIVAEAERLDVKAMGPLVLTEVLFD---EKIREQIKKYRRHFLRFCH 370

Query: 373 AQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQG---VAGA 432
             + +Q  LL  +E        +   ++  +LK +YD+D++EEE ++ W ++        
Sbjct: 371 NNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSK 387

Query: 433 NKSSQIWKYVKPFIEWLQNAESET 446
             + +I    +PFI+WL+ AE E+
Sbjct: 431 ELAKEIRVKAEPFIKWLKEAEEES 387

[Close]
BLAST of Lsi08G009450 vs. TrEMBL
Match: A0A0A0LMB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G310390 PE=4 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 6.4e-229
Identity = 425/448 (94.87%), Postives = 432/448 (96.43%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTD 240
           KKD LKKKST TTKDAVTKVPSKKKNNGSDEDHSPTRSHA DN++AADD+DDDDVQWQTD
Sbjct: 181 KKDVLKKKST-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVSTHG 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKS KKS    EHEKSA NGNKNGNGVSTHG
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS---SEHEKSAANGNKNGNGVSTHG 300

Query: 301 ALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKKKFL 360
           AL+QEIKDYLKKG+S  DLKSFLESL GTRQEIVNALVEALFDGVGKGFSKEV+KKKKFL
Sbjct: 301 ALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360

Query: 361 AAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQGVAG 420
           A  AA EGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYD DVIEEEFVLEWYQQGVAG
Sbjct: 361 AVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEDE 449
           ANKSSQIWKYVKPFIEWLQNAESETE+E
Sbjct: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444

[Close]
BLAST of Lsi08G009450 vs. TrEMBL
Match: A0A059BY17_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03737 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 7.4e-185
Identity = 350/452 (77.43%), Postives = 395/452 (87.39%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKM+TKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG SLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 LKCAACGFLSDVDMRDKLTTFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQ--AADDDDDDDVQWQ 240
           KK++ KKK+++T+KDA +K  S KK N SDE+HSP  S A +N+    ADDDDDDDVQWQ
Sbjct: 181 KKEAAKKKASATSKDA-SKGASTKKKNLSDEEHSPPGSQADENDHHAVADDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE-NKSLKKSPQHPEHEKSAVNGNKNGN-GV 300
           TDTSLEAA+QRIQEQLSA TADMVMLST EE +KS KK P+     ++ VNGN NG    
Sbjct: 241 TDTSLEAARQRIQEQLSAATADMVMLSTNEEKSKSTKKLPEEAAKSETKVNGNGNGTVTA 300

Query: 301 STHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKK 360
            T  +LV EIK++LKKGSS++ LKSFL+SL GT +EI++AL EALFDGVGKGF+KEV KK
Sbjct: 301 ETEDSLVDEIKEFLKKGSSSSQLKSFLKSLSGTPKEIMDALFEALFDGVGKGFAKEVTKK 360

Query: 361 KKFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQ 420
           K +L AA  ++GSQ++LL+AIESFC     E  KEVALVLK LYD+DV+EEEF++EWYQ+
Sbjct: 361 KSYLVAATEEDGSQMVLLQAIESFCGKAGQEVTKEVALVLKFLYDNDVLEEEFIMEWYQK 420

Query: 421 GVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           G+AG NK+S IWK VKPFIEWLQNAESE+E+E
Sbjct: 421 GLAGGNKASPIWKNVKPFIEWLQNAESESEEE 451

[Close]
BLAST of Lsi08G009450 vs. TrEMBL
Match: W9R9S8_9ROSA (Putative eukaryotic translation initiation factor 5-2 OS=Morus notabilis GN=L484_014935 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 3.1e-183
Identity = 354/453 (78.15%), Postives = 395/453 (87.20%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKM+TKIEGRGNGIKTN+VN VEIAKAL R  SYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNIVNNVEIAKALARLPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEELKKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQ-AADDDDDDDVQWQT 240
           KK++ KKK TS +    +K  SKKKNNGSDEDHSPT S A +N+  AA DDDDDDVQWQT
Sbjct: 181 KKEAGKKKGTSGS----SKSSSKKKNNGSDEDHSPTHSQADENDHAAAGDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEE-NKSLKKSPQHPEHEKSAVNGNKNGN-GVS 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE  KS+KKS   PE EK   NGN+NG+ G +
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEEKQKSVKKS---PEREKKPKNGNENGSVGNT 300

Query: 301 THGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKK 360
           T   L +EIK+YLKKGSS + L+SFL SL GT QE+++AL+EALF+GVGKGF+KEV KKK
Sbjct: 301 TKRKLAKEIKEYLKKGSSLSQLQSFLASLSGTPQEVMDALLEALFEGVGKGFAKEVTKKK 360

Query: 361 KFLAAAAAQEG--SQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQ 420
            +LAAA  + G  SQ +LL AIESFC   +P+A KEVALVLK+LYD DV+EEEF+LEWY+
Sbjct: 361 NYLAAAIQEGGSLSQTVLLHAIESFCGKANPDALKEVALVLKALYDGDVLEEEFILEWYE 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           +G+ G N+SSQIWK VKPF+EWLQ+AESE+E+E
Sbjct: 421 KGLVGDNESSQIWKNVKPFVEWLQSAESESEEE 446

[Close]
BLAST of Lsi08G009450 vs. TrEMBL
Match: A0A0B0NJ45_GOSAR (Eukaryotic translation initiation factor 5 OS=Gossypium arboreum GN=F383_17868 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.4e-180
Identity = 348/451 (77.16%), Postives = 382/451 (84.70%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASN DDAFYRYKMPKM+TKIEGRGNGIKTNVVNMVEIAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGASNSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+T
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMLT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPE KK SKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEAKKSSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSTTKDAVTKVP---SKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQW 240
           KK++ KK S++TT    T      +KKK+  SDEDHSPT S A D N+  DDDDDD+VQW
Sbjct: 181 KKEAKKKGSSTTTTSKATAAKGAVTKKKSKHSDEDHSPTHSQA-DENEEVDDDDDDNVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVS 300
           QTDTSLEAA+QRIQEQLSAVTADMVMLST EE KS+KKS   PE E  A       NGV 
Sbjct: 241 QTDTSLEAAQQRIQEQLSAVTADMVMLSTEEEKKSVKKS---PEREVKA-----QENGVI 300

Query: 301 THGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKK 360
            H  LV EIK YL KGSSAA LKSFL SL GT QEI++AL  ALF  VGKGF++EV KKK
Sbjct: 301 AHEKLVDEIKKYLNKGSSAAQLKSFLGSLPGTSQEIMDALFTALFHNVGKGFAEEVTKKK 360

Query: 361 KFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQG 420
            +L AAA +EG Q++LL +IESFC   + EA KEVALVLK+LYD+D++EEEF++EWYQ+G
Sbjct: 361 NYLVAAAREEGWQMMLLNSIESFCGKANLEAAKEVALVLKALYDNDILEEEFIMEWYQKG 420

Query: 421 VAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           VAG NKSSQIWK  KPFIEWLQNAESETE+E
Sbjct: 421 VAGGNKSSQIWKNSKPFIEWLQNAESETEEE 442

[Close]
BLAST of Lsi08G009450 vs. TrEMBL
Match: A0A061FGW1_THECC (Translation initiation factor IF2/IF5 isoform 1 OS=Theobroma cacao GN=TCM_035003 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 3.2e-180
Identity = 344/449 (76.61%), Postives = 381/449 (84.86%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGA+N DDAFYRYKMPKM+TKIEGRGNGIKTNVVNMVEIAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGAANSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITK QMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKMQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPE KK SKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEAKKSSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSTTKDAVTK-VPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQT 240
           KK+S KK +++T+KDA +K   +KKKN  SDEDHSP  S A +N Q A DD D+DVQWQT
Sbjct: 181 KKESKKKGTSTTSKDAASKGAATKKKNKNSDEDHSPAHSQADENEQVASDDGDNDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVSTH 300
           DTSLEAA+QRIQEQLSAVTADMVMLST EE K  K + + PE E     G  + NGVS H
Sbjct: 241 DTSLEAARQRIQEQLSAVTADMVMLSTNEEKK--KTARKSPERE-----GKVHENGVSAH 300

Query: 301 GALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKKKF 360
             LV EI +YLKKGS A  LKSFL SL GT QE+++AL  ALF  V KG +KEV KKK +
Sbjct: 301 EKLVHEIMEYLKKGSPATQLKSFLGSLSGTSQEVMDALFIALFHDVKKGLAKEVTKKKSY 360

Query: 361 LAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQGVA 420
           LAAA  +EG Q++LL +IESFC   SPEA KEVALVLK LYD+D++EEE ++EWYQ+GVA
Sbjct: 361 LAAAIQEEGWQMVLLHSIESFCGKASPEAAKEVALVLKVLYDNDILEEELIMEWYQKGVA 420

Query: 421 GANKSSQIWKYVKPFIEWLQNAESETEDE 449
           G+NKSSQIWK VKPFIEWLQNAESE+E+E
Sbjct: 421 GSNKSSQIWKNVKPFIEWLQNAESESEEE 442

[Close]
BLAST of Lsi08G009450 vs. TAIR10
Match: AT1G36730.1 (AT1G36730.1 Translation initiation factor IF2/IF5)

HSP 1 Score: 549.7 bits (1415), Expect = 1.7e-156
Identity = 302/456 (66.23%), Postives = 357/456 (78.29%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEK GTSLVNGAH+T+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ 
Sbjct: 61  CELGAQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQ 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLT+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +K+
Sbjct: 121 LKCAACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTD 240
           KK++  KK  +TT  +  KV  KK       DHSP RS + +N+QA  ++DDDDVQWQTD
Sbjct: 181 KKEAASKKKAATTGTSKDKVSKKK-------DHSPPRSLSDENDQADSEEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNG---VS 300
           TS EAA++R++EQLSAVTA+MVMLST EE K + +  + PE        ++NGN     +
Sbjct: 241 TSREAAEKRMKEQLSAVTAEMVMLSTVEEKKPVAEVKKAPEQV------HENGNSKIPEN 300

Query: 301 THGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKK 360
            H  LV EIK+ L  GSS   LK+ L S     QE ++AL  ALF G GKGF+KEV+KKK
Sbjct: 301 AHEKLVNEIKELLSSGSSPTQLKTALASNSANPQEKMDALFSALFGGTGKGFAKEVIKKK 360

Query: 361 KFLAA-----AAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLE 420
           K+L A       A   +Q+ LL  IESFC+  S EA KEVALV+K LYD D+++E+ ++E
Sbjct: 361 KYLLALMMMQEEAGAPAQMGLLNGIESFCMKASAEAAKEVALVIKGLYDEDILDEDVIVE 420

Query: 421 WYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           WY +GV    KSS + K V PFIEWLQNAESE+E+E
Sbjct: 421 WYNKGV----KSSPVLKNVTPFIEWLQNAESESEEE 439

[Close]
BLAST of Lsi08G009450 vs. TAIR10
Match: AT1G77840.1 (AT1G77840.1 Translation initiation factor IF2/IF5)

HSP 1 Score: 547.0 bits (1408), Expect = 1.1e-155
Identity = 310/456 (67.98%), Postives = 361/456 (79.17%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNR+DAFYRYKMPKMVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFG
Sbjct: 1   MALQNIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ 
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVN 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGFIS+VDMRDKLT FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+K+
Sbjct: 121 LKCAACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQA---ADDDDDDDVQW 240
           K    KKK+ S  KD+ T      KN+ SDED SP      D N      D+DDDD V+W
Sbjct: 181 K---AKKKALSNGKDSKT-----SKNHSSDEDISPKH----DENALEVDEDEDDDDGVEW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGN--KNGNG 300
           QTDTS EAA++R+ EQLSA TA+MVMLS  E  +  KK+P      KS  NGN  K  N 
Sbjct: 241 QTDTSREAAEKRMMEQLSAKTAEMVMLSAMEVEE--KKAP------KSKSNGNVVKTENP 300

Query: 301 VSTHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMK 360
                 LVQ++K+YLKKGS  + LKSF+ SL    Q+I++AL  ALFDGVGKGF+KEV K
Sbjct: 301 PPQEKNLVQDMKEYLKKGSPISALKSFISSLSEPPQDIMDALFNALFDGVGKGFAKEVTK 360

Query: 361 KKKFLAAAAA--QEGSQIILLRAIESFC-LNTSPEAGKEVALVLKSLYDSDVIEEEFVLE 420
           KK +LAAAA   ++GSQ+ LL +I +FC  N + EA KEVALVLK+LYD D+IEEE VL+
Sbjct: 361 KKNYLAAAATMQEDGSQMHLLNSIGTFCGKNGNEEALKEVALVLKALYDQDIIEEEVVLD 420

Query: 421 WYQQGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           WY++G+ GA+KSS +WK VKPF+EWLQ+AESE+E+E
Sbjct: 421 WYEKGLTGADKSSPVWKNVKPFVEWLQSAESESEEE 436

[Close]
BLAST of Lsi08G009450 vs. TAIR10
Match: AT2G34970.1 (AT2G34970.1 Trimeric LpxA-like enzyme)

HSP 1 Score: 54.3 bits (129), Expect = 2.2e-07
Identity = 34/103 (33.01%), Postives = 55/103 (53.40%), Query Frame = 1 

Query: 347 KGFSKEVMKKKKFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIE 406
           K  +  + K K  L   A +   QI ++   E  C  +  E G     +L  LYD DV++
Sbjct: 619 KNAASIITKWKDLLGFYAKKIDEQIEVIMKFEEMCQESHKELGPLFTQILHLLYDKDVLQ 678

Query: 407 EEFVLEWYQQGVAGANKSSQIW-KYVKPFIEWLQNAESETEDE 449
           E+ +L W ++  AGA+++ +++ K    FI+WL+ A SE EDE
Sbjct: 679 EDAILRW-EEEKAGADEADKVYLKQCDTFIQWLKEA-SEEEDE 719

[Close]
BLAST of Lsi08G009450 vs. TAIR10
Match: AT5G20920.1 (AT5G20920.1 eukaryotic translation initiation factor 2 beta subunit)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 1 

Query: 35  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHETAKLAGLLENF 94
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 95  IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 128
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

[Close]
BLAST of Lsi08G009450 vs. NCBI nr
Match: gi|449465960|ref|XP_004150695.1| (PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis sativus])

HSP 1 Score: 801.2 bits (2068), Expect = 9.2e-229
Identity = 425/448 (94.87%), Postives = 432/448 (96.43%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTD 240
           KKD LKKKST TTKDAVTKVPSKKKNNGSDEDHSPTRSHA DN++AADD+DDDDVQWQTD
Sbjct: 181 KKDVLKKKST-TTKDAVTKVPSKKKNNGSDEDHSPTRSHADDNDRAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVSTHG 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKS KKS    EHEKSA NGNKNGNGVSTHG
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS---SEHEKSAANGNKNGNGVSTHG 300

Query: 301 ALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKKKFL 360
           AL+QEIKDYLKKG+S  DLKSFLESL GTRQEIVNALVEALFDGVGKGFSKEV+KKKKFL
Sbjct: 301 ALIQEIKDYLKKGASTTDLKSFLESLAGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360

Query: 361 AAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQGVAG 420
           A  AA EGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYD DVIEEEFVLEWYQQGVAG
Sbjct: 361 AVVAAVEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYQQGVAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEDE 449
           ANKSSQIWKYVKPFIEWLQNAESETE+E
Sbjct: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444

[Close]
BLAST of Lsi08G009450 vs. NCBI nr
Match: gi|659089161|ref|XP_008445359.1| (PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo])

HSP 1 Score: 796.2 bits (2055), Expect = 3.0e-227
Identity = 421/448 (93.97%), Postives = 432/448 (96.43%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI
Sbjct: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQWQTD 240
           KKD LKKKST TTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDN+QAADD+DDDDVQWQTD
Sbjct: 181 KKDVLKKKST-TTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNDQAADDEDDDDVQWQTD 240

Query: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVSTHG 300
           TSLEAAKQRIQEQLSAVTADMVMLSTTEENKS KKS    EHEKSA NGNKNGNGVSTH 
Sbjct: 241 TSLEAAKQRIQEQLSAVTADMVMLSTTEENKSSKKS---SEHEKSAANGNKNGNGVSTHV 300

Query: 301 ALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKKKFL 360
           +L+QEIKD+LKKGSS  DLKSFLESL GTRQEIVNALVEALFDGVGKGFSKEV+KKKKFL
Sbjct: 301 SLIQEIKDHLKKGSSTTDLKSFLESLSGTRQEIVNALVEALFDGVGKGFSKEVVKKKKFL 360

Query: 361 AAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQGVAG 420
           A  AA+EGSQI LLR+IESFC+NTSPEAGKEVALVLKSLYD DVIEEEFVLEWY+QGVAG
Sbjct: 361 AVVAAEEGSQITLLRSIESFCINTSPEAGKEVALVLKSLYDGDVIEEEFVLEWYKQGVAG 420

Query: 421 ANKSSQIWKYVKPFIEWLQNAESETEDE 449
           ANKSSQIWKYVKPFIEWLQNAESETE+E
Sbjct: 421 ANKSSQIWKYVKPFIEWLQNAESETEEE 444

[Close]
BLAST of Lsi08G009450 vs. NCBI nr
Match: gi|702379840|ref|XP_010063324.1| (PREDICTED: probable eukaryotic translation initiation factor 5-1 [Eucalyptus grandis])

HSP 1 Score: 654.8 bits (1688), Expect = 1.1e-184
Identity = 350/452 (77.43%), Postives = 395/452 (87.39%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKM+TKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTG SLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGISLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEEQKK+
Sbjct: 121 LKCAACGFLSDVDMRDKLTTFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEEQKKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQ--AADDDDDDDVQWQ 240
           KK++ KKK+++T+KDA +K  S KK N SDE+HSP  S A +N+    ADDDDDDDVQWQ
Sbjct: 181 KKEAAKKKASATSKDA-SKGASTKKKNLSDEEHSPPGSQADENDHHAVADDDDDDDVQWQ 240

Query: 241 TDTSLEAAKQRIQEQLSAVTADMVMLSTTEE-NKSLKKSPQHPEHEKSAVNGNKNGN-GV 300
           TDTSLEAA+QRIQEQLSA TADMVMLST EE +KS KK P+     ++ VNGN NG    
Sbjct: 241 TDTSLEAARQRIQEQLSAATADMVMLSTNEEKSKSTKKLPEEAAKSETKVNGNGNGTVTA 300

Query: 301 STHGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKK 360
            T  +LV EIK++LKKGSS++ LKSFL+SL GT +EI++AL EALFDGVGKGF+KEV KK
Sbjct: 301 ETEDSLVDEIKEFLKKGSSSSQLKSFLKSLSGTPKEIMDALFEALFDGVGKGFAKEVTKK 360

Query: 361 KKFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQ 420
           K +L AA  ++GSQ++LL+AIESFC     E  KEVALVLK LYD+DV+EEEF++EWYQ+
Sbjct: 361 KSYLVAATEEDGSQMVLLQAIESFCGKAGQEVTKEVALVLKFLYDNDVLEEEFIMEWYQK 420

Query: 421 GVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           G+AG NK+S IWK VKPFIEWLQNAESE+E+E
Sbjct: 421 GLAGGNKASPIWKNVKPFIEWLQNAESESEEE 451

[Close]
BLAST of Lsi08G009450 vs. NCBI nr
Match: gi|703095323|ref|XP_010095506.1| (putative eukaryotic translation initiation factor 5-2 [Morus notabilis])

HSP 1 Score: 649.4 bits (1674), Expect = 4.5e-183
Identity = 354/453 (78.15%), Postives = 395/453 (87.20%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASNRDDAFYRYKMPKM+TKIEGRGNGIKTN+VN VEIAKAL R  SYTTKYFG
Sbjct: 1   MALQNIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNIVNNVEIAKALARLPSYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEE KK+
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEELKKL 180

Query: 181 KKDSLKKKSTSTTKDAVTKVPSKKKNNGSDEDHSPTRSHAGDNNQ-AADDDDDDDVQWQT 240
           KK++ KKK TS +    +K  SKKKNNGSDEDHSPT S A +N+  AA DDDDDDVQWQT
Sbjct: 181 KKEAGKKKGTSGS----SKSSSKKKNNGSDEDHSPTHSQADENDHAAAGDDDDDDVQWQT 240

Query: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEE-NKSLKKSPQHPEHEKSAVNGNKNGN-GVS 300
           DTSLEAAKQRIQEQLSAVTADMVMLSTTEE  KS+KKS   PE EK   NGN+NG+ G +
Sbjct: 241 DTSLEAAKQRIQEQLSAVTADMVMLSTTEEKQKSVKKS---PEREKKPKNGNENGSVGNT 300

Query: 301 THGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKK 360
           T   L +EIK+YLKKGSS + L+SFL SL GT QE+++AL+EALF+GVGKGF+KEV KKK
Sbjct: 301 TKRKLAKEIKEYLKKGSSLSQLQSFLASLSGTPQEVMDALLEALFEGVGKGFAKEVTKKK 360

Query: 361 KFLAAAAAQEG--SQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQ 420
            +LAAA  + G  SQ +LL AIESFC   +P+A KEVALVLK+LYD DV+EEEF+LEWY+
Sbjct: 361 NYLAAAIQEGGSLSQTVLLHAIESFCGKANPDALKEVALVLKALYDGDVLEEEFILEWYE 420

Query: 421 QGVAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           +G+ G N+SSQIWK VKPF+EWLQ+AESE+E+E
Sbjct: 421 KGLVGDNESSQIWKNVKPFVEWLQSAESESEEE 446

[Close]
BLAST of Lsi08G009450 vs. NCBI nr
Match: gi|728833402|gb|KHG12845.1| (Eukaryotic translation initiation factor 5 [Gossypium arboreum])

HSP 1 Score: 640.6 bits (1651), Expect = 2.1e-180
Identity = 348/451 (77.16%), Postives = 382/451 (84.70%), Query Frame = 1 

Query: 1   MALQNIGASNRDDAFYRYKMPKMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFG 60
           MALQNIGASN DDAFYRYKMPKM+TKIEGRGNGIKTNVVNMVEIAKAL RPASYTTKYFG
Sbjct: 1   MALQNIGASNSDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALARPASYTTKYFG 60

Query: 61  CELGAQSKFDEKTGTSLVNGAHETAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT 120
           CELGAQSKFDEKTGTSLVNGAH+TAKLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+T
Sbjct: 61  CELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIVITKTQMLT 120

Query: 121 MKCAACGFISDVDMRDKLTTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKI 180
           +KCAACGF+SDVDMRDKLTTFILKNPPE KK SKDKKAMRRAEKERLKEGEAADEE KKI
Sbjct: 121 LKCAACGFVSDVDMRDKLTTFILKNPPEAKKSSKDKKAMRRAEKERLKEGEAADEELKKI 180

Query: 181 KKDSLKKKSTSTTKDAVTKVP---SKKKNNGSDEDHSPTRSHAGDNNQAADDDDDDDVQW 240
           KK++ KK S++TT    T      +KKK+  SDEDHSPT S A D N+  DDDDDD+VQW
Sbjct: 181 KKEAKKKGSSTTTTSKATAAKGAVTKKKSKHSDEDHSPTHSQA-DENEEVDDDDDDNVQW 240

Query: 241 QTDTSLEAAKQRIQEQLSAVTADMVMLSTTEENKSLKKSPQHPEHEKSAVNGNKNGNGVS 300
           QTDTSLEAA+QRIQEQLSAVTADMVMLST EE KS+KKS   PE E  A       NGV 
Sbjct: 241 QTDTSLEAAQQRIQEQLSAVTADMVMLSTEEEKKSVKKS---PEREVKA-----QENGVI 300

Query: 301 THGALVQEIKDYLKKGSSAADLKSFLESLFGTRQEIVNALVEALFDGVGKGFSKEVMKKK 360
            H  LV EIK YL KGSSAA LKSFL SL GT QEI++AL  ALF  VGKGF++EV KKK
Sbjct: 301 AHEKLVDEIKKYLNKGSSAAQLKSFLGSLPGTSQEIMDALFTALFHNVGKGFAEEVTKKK 360

Query: 361 KFLAAAAAQEGSQIILLRAIESFCLNTSPEAGKEVALVLKSLYDSDVIEEEFVLEWYQQG 420
            +L AAA +EG Q++LL +IESFC   + EA KEVALVLK+LYD+D++EEEF++EWYQ+G
Sbjct: 361 NYLVAAAREEGWQMMLLNSIESFCGKANLEAAKEVALVLKALYDNDILEEEFIMEWYQKG 420

Query: 421 VAGANKSSQIWKYVKPFIEWLQNAESETEDE 449
           VAG NKSSQIWK  KPFIEWLQNAESETE+E
Sbjct: 421 VAGGNKSSQIWKNSKPFIEWLQNAESETEEE 442

The following BLAST results are available for this feature:
BLAST of Lsi08G009450 vs. Swiss-Prot
Match NameE-valueIdentityDescription
IF5_PHAVU2.0e-15968.58Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris GN=EIF5 PE=2 SV... [more]
IF5_MAIZE1.0e-15566.96Eukaryotic translation initiation factor 5 OS=Zea mays GN=EIF5 PE=2 SV=1[more]
IF5Y_ARATH3.0e-15566.23Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana GN... [more]
IF5Z_ARATH2.0e-15467.98Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana GN... [more]
IF5_RAT3.6e-4731.98Eukaryotic translation initiation factor 5 OS=Rattus norvegicus GN=Eif5 PE=1 SV=... [more]
BLAST of Lsi08G009450 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LMB4_CUCSA6.4e-22994.87Uncharacterized protein OS=Cucumis sativus GN=Csa_2G310390 PE=4 SV=1[more]
A0A059BY17_EUCGR7.4e-18577.43Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03737 PE=4 SV=1[more]
W9R9S8_9ROSA3.1e-18378.15Putative eukaryotic translation initiation factor 5-2 OS=Morus notabilis GN=L484... [more]
A0A0B0NJ45_GOSAR1.4e-18077.16Eukaryotic translation initiation factor 5 OS=Gossypium arboreum GN=F383_17868 P... [more]
A0A061FGW1_THECC3.2e-18076.61Translation initiation factor IF2/IF5 isoform 1 OS=Theobroma cacao GN=TCM_035003... [more]
BLAST of Lsi08G009450 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G36730.11.7e-15666.23 Translation initiation factor IF2/IF5[more]
AT1G77840.11.1e-15567.98 Translation initiation factor IF2/IF5[more]
AT2G34970.12.2e-0733.01 Trimeric LpxA-like enzyme[more]
AT5G20920.12.9e-0729.03 eukaryotic translation initiation factor 2 beta subunit[more]
BLAST of Lsi08G009450 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449465960|ref|XP_004150695.1|9.2e-22994.87PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis sativus][more]
gi|659089161|ref|XP_008445359.1|3.0e-22793.97PREDICTED: eukaryotic translation initiation factor 5-like [Cucumis melo][more]
gi|702379840|ref|XP_010063324.1|1.1e-18477.43PREDICTED: probable eukaryotic translation initiation factor 5-1 [Eucalyptus gra... [more]
gi|703095323|ref|XP_010095506.1|4.5e-18378.15putative eukaryotic translation initiation factor 5-2 [Morus notabilis][more]
gi|728833402|gb|KHG12845.1|2.1e-18077.16Eukaryotic translation initiation factor 5 [Gossypium arboreum][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0005515protein binding
GO:0003743translation initiation factor activity
Vocabulary: Biological Process
TermDefinition
GO:0006413translational initiation
Vocabulary: INTERPRO
TermDefinition
IPR016190Transl_init_fac_IF2/IF5_Zn-bd
IPR016189Transl_init_fac_IF2/IF5_N
IPR016024ARM-type_fold
IPR016021MIF4-like_sf
IPR003307W2_domain
IPR002735Transl_init_fac_IF2/IF5
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006446 regulation of translational initiation
biological_process GO:0006413 translational initiation
cellular_component GO:0005840 ribosome
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0005488 binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G009450.1Lsi08G009450.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002735Translation initiation factor IF2/IF5PFAMPF01873eIF-5_eIF-2Bcoord: 11..127
score: 3.5
IPR002735Translation initiation factor IF2/IF5SMARTSM00653eIF2Bneu4coord: 15..129
score: 9.2
IPR003307W2 domainPFAMPF02020W2coord: 370..448
score: 3.2
IPR003307W2 domainSMARTSM00515542_3coord: 357..444
score: 1.9
IPR003307W2 domainPROFILEPS51363W2coord: 289..448
score: 24
IPR016021MIF4G-like domainGENE3DG3DSA:1.25.40.180coord: 302..448
score: 1.2
IPR016024Armadillo-type foldunknownSSF48371ARM repeatcoord: 301..442
score: 8.92
IPR016189Translation initiation factor IF2/IF5, N-terminalGENE3DG3DSA:3.30.30.50coord: 30..72
score: 3.7
IPR016189Translation initiation factor IF2/IF5, N-terminalunknownSSF100966Translation initiation factor 2 beta, aIF2beta, N-terminal domaincoord: 10..101
score: 9.55
IPR016190Translation initiation factor IF2/IF5, zinc-bindingunknownSSF75689Zinc-binding domain of translation initiation factor 2 betacoord: 98..133
score: 2.0
NoneNo IPR availableunknownCoilCoilcoord: 159..179
scor
NoneNo IPR availablePANTHERPTHR23001EUKARYOTIC TRANSLATION INITIATION FACTORcoord: 208..314
score: 5.9E-259coord: 1..191
score: 5.9E-259coord: 331..448
score: 5.9E
NoneNo IPR availablePANTHERPTHR23001:SF8EUKARYOTIC TRANSLATION INITIATION FACTOR 5-2-RELATEDcoord: 1..191
score: 5.9E-259coord: 331..448
score: 5.9E-259coord: 208..314
score: 5.9E