Cp4.1LG07g01200 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG07g01200
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionEukaryotic translation initiation factor 5
LocationCp4.1LG07 : 668781 .. 671351 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGTGACGTGTAGGGTTTTCTTTCTACCATTGATTTGAGTGTGTATAAAAGGCGTTCCTTCTGCTCAACAGAATCCCATCGGCATCTTCGTCACCGCGAAATCAAGAGCCAGAGCTTCAGGATCAGTTTGTTTTTCCTTCTCTTATCACGACAGCCTAACACTGTAAGACGATAATTTCCTATGTTGTAATTTGTTTGTTAATCTAACTTCATTATTGTTTCTAGTTTCTATTTTGGCCTCAGATTTTCAACTCATGTTTTGAGTTTGTTATTTTTGTTTTGTTGTAATTGATTATATAAGTTCATACGCCTTTGATTCAATGATATCCTCACGATCGGTTCTTCGATTATTGATTTCTGATGGGAATTGTAAGTTTCTTGCTGCGTCGTGTTCAGATCTGTGGATTTAGTTGCCTGTTTCTCGTTTGTTTTGGATGTTCTTTTTTATTTCATCTCTCGATGATCGTAATTGAAAATTATGATTGTCTCCGTAGATTGGATATTCATGTAAAGCTGATTGAATCTTCTCTGCGAATTCGATTTTATTATTCTCCATCTAGTATTTTCATGCGTTGAATCTCGTCAGTTGTGTATTGGATGGATTGTCAAGTTTTAAGGTAGAGACAGACTTATATTTTGTGGCTGATTGTGTTATTTTTTTCATCTTCTAAAATCGAACTTTATTTAAAGTGAACTGCAATTTTGTTTATGTTTTCATTGCTGCATTTAAAGATTACTCCATTGAGATCAATAAATCTGCTGTTGTACGGTTCAGTTTTCTATACCAAAGGTAGAAATCAGCTGTTAAATGATATTTGTGCACTTTAACTTGCGCTTGATTGATGATGTTTTATGTTCACTGTCAACGTTTAAGTTCATGAGAATCGAGCATAATTATTTGTTCTTAATCCACAGGAAGTTGGCCTTCTGGTCTGCTGTTGTGTAGTTCAAAGTGGAGAGCGTGAAGTTTGAGACTCCTTTTATGTCTGAACGGGCCACTCTCTTTTTGTCATCGATTGGATTGCTTGGAGAGTATTGTCCTACGTCTAGGAAAAAGTACAGCTGCGCTGAGACTATTGGCTGCATGCATCTTGAGTTGCCTGGTGGTCATAGTAACAGCCCGCGGGTGGTAGCCTTGTCCAGCTGCTCAAAGAACAACAGGGCATCTTAAAGATACCACTGCTGATATCTATCTTTTCTAACCACTACTGCCGTTTTTGTGTTCTAACATGGCGTTGCAGAATATTGGTGCCTCAAACCGTGACGATGCCTTCTACAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATTAAGACCAATGTGGTTAACATGGTGGAAATTGCCAAAGCCTTGGGCAGGCCTGCTTCTTACACTACCAAGTACTTTGGATGTGAGCTTGGTGCACAATCCAAATTCGATGAGAAAACTGGGACTTCTCTTGTAAATGGTGCACACGATACAGCCAAGCTGGCTGGTCTTCTCGAGAACTTTATCAAGAAGTATGTTCAATGCTACGGATGTGGTAACCCCGAAACTGAGATTATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTTATATCTGATGTTGACATGAGGGATAAAATCACAACATTTATTCTCAAGAATCCTCCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCTATGAGGCGGGCTGAGAAGGAAAGACTTAAGGAGGGCGAGGCTGCTGATGAGGAGCAGAAGAAGCTAAAGAAGGATTCGGTGAAGAAGAAAAGCACGAGTGGTAATTCAAAGGAAACTGTTACTAAAGTGCCAAGCAAGAAGAAGAATAATGGCTCGGATGACGATCATTCCCCAGTTCCTATCCATGCTAGTGGTAAGGACGAAGTAGCTGATGATGATGACGATGATGACGATGTGCAGTGGCAAACGGACACATCTTTAGAGGCAGCTAAGCAGCGTATTCAGGAGCAGTTGAGTGCAGTCACTGCTGATATGGTCATGCTGTCTACCGAAGAAACTACGTCGTCCAAAAAGCCCTCTGAACATGAGAAGTCTTCGATCAACGGAAACAAGAACGGAAATGGAGTGTCTACCCATGTTGCTCTTGTCAACGAGATCAAAGATTATTTGAAGAAAGGTTCCTCCGCTGCCAACCTCAAATCATTTTTGGGATCACTTTCTGGAACTCCCCAGGAGATTGTTAATGCCTTAGTTGAGGCTCTGTTTGATGGCGTTGGAAAAGGCTTCTCAAAGGAGGTGATGAAGAAGAAGAACTTCTTGGCTGCAGCTGTTGCTCTGGAGGGATCACAGATAGCTCTATTGCGTGCCATTGAATCTTTCTGTCTCAACACGAGTTCGGAGGCTGCTAAGGAGGTTGCTCTGGTTCTGAAATCGCTATACGACAGCAACCTGATCGAGGAGGAATTTGTATTAGAGTGGTACCAGAAGGGCGTGGTTGGCGCTAACAAAAGTTCTCCAATCTGGAACCATGTAAAGCCGTTCATCGAATGGCTCCAGAACGCCGAGTCCGAGACCGAAGAAGAGTAA

mRNA sequence

TTGGTGACGTGTAGGGTTTTCTTTCTACCATTGATTTGAGTGTGTATAAAAGGCGTTCCTTCTGCTCAACAGAATCCCATCGGCATCTTCGTCACCGCGAAATCAAGAGCCAGAGCTTCAGGATCAGTTTGTTTTTCCTTCTCTTATCACGACAGCCTAACACTGAAAAAGTACAGCTGCGCTGAGACTATTGGCTGCATGCATCTTGAGTTGCCTGGTGGTCATAGTAACAGCCCGCGGGTGAATATTGGTGCCTCAAACCGTGACGATGCCTTCTACAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATTAAGACCAATGTGGTTAACATGGTGGAAATTGCCAAAGCCTTGGGCAGGCCTGCTTCTTACACTACCAAGTACTTTGGATGTGAGCTTGGTGCACAATCCAAATTCGATGAGAAAACTGGGACTTCTCTTGTAAATGGTGCACACGATACAGCCAAGCTGGCTGGTCTTCTCGAGAACTTTATCAAGAAGTATGTTCAATGCTACGGATGTGGTAACCCCGAAACTGAGATTATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTTATATCTGATGTTGACATGAGGGATAAAATCACAACATTTATTCTCAAGAATCCTCCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCTATGAGGCGGGCTGAGAAGGAAAGACTTAAGGAGGGCGAGGCTGCTGATGAGGAGCAGAAGAAGCTAAAGAAGGATTCGGTGAAGAAGAAAAGCACGAGTGGTAATTCAAAGGAAACTGTTACTAAATTGAGTGCAGTCACTGCTGATATGGTCATGCTGTCTACCGAAGAAACTACGTCGTCCAAAAAGCCCTCTGAACATGAGAAGTCTTCGATCAACGGAAACAAGAACGGAAATGGAGTGTCTACCCATGTTGCTCTTGTCAACGAGATCAAAGATTATTTGAAGAAAGGTTCCTCCGCTGCCAACCTCAAATCATTTTTGGGATCACTTTCTGGAACTCCCCAGGAGATTGTTAATGCCTTAGTTGAGGCTCTGTTTGATGGCGTTGGAAAAGGCTTCTCAAAGGAGGTGATGAAGAAGAAGAACTTCTTGGCTGCAGCTGTTGCTCTGGAGGGATCACAGATAGCTCTATTGCGTGCCATTGAATCTTTCTGTCTCAACACGAGTTCGGAGGCTGCTAAGGAGGTTGCTCTGGTTCTGAAATCGCTATACGACAGCAACCTGATCGAGGAGGAATTTGTATTAGAGTGGTACCAGAAGGGCGTGGTTGGCGCTAACAAAAGTTCTCCAATCTGGAACCATGTAAAGCCGTTCATCGAATGGCTCCAGAACGCCGAGTCCGAGACCGAAGAAGAGTAA

Coding sequence (CDS)

ATGCATCTTGAGTTGCCTGGTGGTCATAGTAACAGCCCGCGGGTGAATATTGGTGCCTCAAACCGTGACGATGCCTTCTACAGGTATAAGATGCCTAGGATGGTTACTAAAATTGAAGGTAGAGGTAATGGCATTAAGACCAATGTGGTTAACATGGTGGAAATTGCCAAAGCCTTGGGCAGGCCTGCTTCTTACACTACCAAGTACTTTGGATGTGAGCTTGGTGCACAATCCAAATTCGATGAGAAAACTGGGACTTCTCTTGTAAATGGTGCACACGATACAGCCAAGCTGGCTGGTCTTCTCGAGAACTTTATCAAGAAGTATGTTCAATGCTACGGATGTGGTAACCCCGAAACTGAGATTATCATCACGAAGACGCAGATGATCACTATGAAATGTGCTGCCTGTGGATTTATATCTGATGTTGACATGAGGGATAAAATCACAACATTTATTCTCAAGAATCCTCCTGAACAGAAGAAGGGATCAAAAGACAAGAAAGCTATGAGGCGGGCTGAGAAGGAAAGACTTAAGGAGGGCGAGGCTGCTGATGAGGAGCAGAAGAAGCTAAAGAAGGATTCGGTGAAGAAGAAAAGCACGAGTGGTAATTCAAAGGAAACTGTTACTAAATTGAGTGCAGTCACTGCTGATATGGTCATGCTGTCTACCGAAGAAACTACGTCGTCCAAAAAGCCCTCTGAACATGAGAAGTCTTCGATCAACGGAAACAAGAACGGAAATGGAGTGTCTACCCATGTTGCTCTTGTCAACGAGATCAAAGATTATTTGAAGAAAGGTTCCTCCGCTGCCAACCTCAAATCATTTTTGGGATCACTTTCTGGAACTCCCCAGGAGATTGTTAATGCCTTAGTTGAGGCTCTGTTTGATGGCGTTGGAAAAGGCTTCTCAAAGGAGGTGATGAAGAAGAAGAACTTCTTGGCTGCAGCTGTTGCTCTGGAGGGATCACAGATAGCTCTATTGCGTGCCATTGAATCTTTCTGTCTCAACACGAGTTCGGAGGCTGCTAAGGAGGTTGCTCTGGTTCTGAAATCGCTATACGACAGCAACCTGATCGAGGAGGAATTTGTATTAGAGTGGTACCAGAAGGGCGTGGTTGGCGCTAACAAAAGTTCTCCAATCTGGAACCATGTAAAGCCGTTCATCGAATGGCTCCAGAACGCCGAGTCCGAGACCGAAGAAGAGTAA

Protein sequence

MHLELPGGHSNSPRVNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCAACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDSVKKKSTSGNSKETVTKLSAVTADMVMLSTEETTSSKKPSEHEKSSINGNKNGNGVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVGKGFSKEVMKKKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLIEEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE
BLAST of Cp4.1LG07g01200 vs. Swiss-Prot
Match: IF5Y_ARATH (Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana GN=At1g36730 PE=2 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 3.3e-92
Identity = 171/198 (86.36%), Postives = 188/198 (94.95%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFGCELG
Sbjct: 5   NIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEK GTSLVNGAHDT+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ +KCA
Sbjct: 65  AQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKD- 195
           ACGF+SDVDMRDK+T+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +KLKK+ 
Sbjct: 125 ACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKLKKEA 184

Query: 196 -SVKKKSTSGNSKETVTK 212
            S KK +T+G SK+ V+K
Sbjct: 185 ASKKKAATTGTSKDKVSK 202

BLAST of Cp4.1LG07g01200 vs. Swiss-Prot
Match: IF5_MAIZE (Eukaryotic translation initiation factor 5 OS=Zea mays GN=EIF5 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.3e-92
Identity = 190/297 (63.97%), Postives = 219/297 (73.74%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFGCELG
Sbjct: 5   NIGASNRDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTG SLVNGAHDTAKLAGLLE FIKKYVQCYGCGNPETEI+I+KTQMI++KCA
Sbjct: 65  AQSKFDEKTGISLVNGAHDTAKLAGLLEVFIKKYVQCYGCGNPETEILISKTQMISLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+TTFILKNPPEQKKG KDKKAMRRAEKERLKEGEAADEEQKKLKKD+
Sbjct: 125 ACGFVSDVDMRDKLTTFILKNPPEQKKGGKDKKAMRRAEKERLKEGEAADEEQKKLKKDA 184

Query: 196 VKKKSTSGNSKETVTKLSAVTADMVMLSTEETTSSKKPSEHEKSSINGNKNGNGVSTHV- 255
            KK S    +K    K +  T       ++E  SS     H+      + + + V     
Sbjct: 185 KKKGSKDSTAKGLKKKATTAT------GSDEDHSSSPTRSHDGDKAAADDDDDDVQWQTD 244

Query: 256 ALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVGKGFSKEVMKKK 312
             +   K  +++  SAA  +  + S   T +++     +   DG   G +KE+   K
Sbjct: 245 TSIEAAKQRMQEQLSAATAEMVMLSTEETEKKMKQPTHK---DGSTNGSAKEIPNDK 292

BLAST of Cp4.1LG07g01200 vs. Swiss-Prot
Match: IF5_PHAVU (Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris GN=EIF5 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.4e-87
Identity = 174/238 (73.11%), Postives = 196/238 (82.35%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA N DDAFYRYKMPRMVTKIEGRGNGIKTNVVNMV+IAK L RPASYTTKYFGCELG
Sbjct: 5   NIGAGNSDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVDIAKRLARPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTGTS VNGAH+TAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QMI +KCA
Sbjct: 65  AQSKFDEKTGTSHVNGAHETAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMIQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+TTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+KK+ 
Sbjct: 125 ACGFVSDVDMRDKLTTFIVKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKVKKEV 184

Query: 196 VKKKSTS---GNSKETVTKLSAVTADMVMLSTEETTSSKKPSEHEKSSINGNKNGNGV 251
            KK S+S   G +K T++K     +D        + + K+  E E++    + + +GV
Sbjct: 185 KKKGSSSAKDGTAKSTISKKKGSGSD----EDRRSPTHKQIEEKEEAKDEDDDDDDGV 238

BLAST of Cp4.1LG07g01200 vs. Swiss-Prot
Match: IF5Z_ARATH (Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana GN=At1g77840 PE=2 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.3e-85
Identity = 162/195 (83.08%), Postives = 179/195 (91.79%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFGCELG
Sbjct: 5   NIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTGTSLVNGAH+T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ +KCA
Sbjct: 65  AQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVNLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGFIS+VDMRDK+T FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+KLK   
Sbjct: 125 ACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKLK--- 184

Query: 196 VKKKSTSGNSKETVT 211
            KKK+ S N K++ T
Sbjct: 185 AKKKALS-NGKDSKT 195

BLAST of Cp4.1LG07g01200 vs. Swiss-Prot
Match: IF5_MOUSE (Eukaryotic translation initiation factor 5 OS=Mus musculus GN=Eif5 PE=1 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 2.7e-46
Identity = 141/414 (34.06%), Postives = 211/414 (50.97%), Query Frame = 1

Query: 15  VNIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCEL 74
           VN+  S  D  FYRYKMPR++ K+EG+GNGIKT +VNMV++AKAL RP +Y TKYFGCEL
Sbjct: 3   VNVNRSVSDQ-FYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCEL 62

Query: 75  GAQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIIT-KTQMITMK 134
           GAQ++FD K    +VNG+H+  KL  +L+ FIKK+V C  C NPET++ +  K Q I   
Sbjct: 63  GAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNS 122

Query: 135 CAACGFISDVDMRDKITTFILKNPPEQK---KGSKDKKAMRRAEKERLKEGEAADEEQKK 194
           C ACG+   +D   K+ TFILKNPPE      G K+K+   R  K++     +  E    
Sbjct: 123 CKACGYRGMLDTHHKLCTFILKNPPENSDIGTGKKEKEKKNRKGKDKENGSVSTSETPPP 182

Query: 195 LKKDSVKKKSTSGNSKETVTKLSAVTADMVMLSTEETTSSK--KPSEHEKSSINGNKNGN 254
              + +         ++          D    +TEE    +  + S+H K     +    
Sbjct: 183 PPPNEISPPHAVEEEEDD---------DWGEDTTEEAQRRRMDEISDHAKGLTLSDDLER 242

Query: 255 GVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVN-------------ALVEAL 314
            V      VN + D++KK       K   G +  + +EIV               L E L
Sbjct: 243 TVEER---VNILFDFVKK-------KKEEGIIDSSDKEIVAEAERLDVKAMGPLVLTEVL 302

Query: 315 FDGVGKGFSKEVMK-KKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALVLKSLY 374
           FD   +   +++ K +++FL      + +Q  LL  +E       ++   ++  +LK +Y
Sbjct: 303 FD---EKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMY 362

Query: 375 DSNLIEEEFVLEWYQKG---VVGANKSSPIWNHVKPFIEWLQNAESET---EEE 403
           D++L+EEE ++ W +K     V    +  I    +PFI+WL+ AE E+   EEE
Sbjct: 363 DADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEE 393

BLAST of Cp4.1LG07g01200 vs. TrEMBL
Match: A0A059BWC9_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03737 PE=4 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 6.3e-159
Identity = 306/413 (74.09%), Postives = 342/413 (82.81%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG
Sbjct: 5   NIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTG SLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT+KCA
Sbjct: 65  AQSKFDEKTGISLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+TTFILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEEQKKLKK++
Sbjct: 125 ACGFLSDVDMRDKLTTFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEEQKKLKKEA 184

Query: 196 VKKKSTSGN---SKETVTK-----------------LSAVTADMVMLSTEETTSS---KK 255
            KKK+++ +   SK   TK                 LSA TADMVMLST E  S    K 
Sbjct: 185 AKKKASATSKDASKGASTKKKNLSDEEHSPPGSQEQLSAATADMVMLSTNEEKSKSTKKL 244

Query: 256 PSEHEKSSINGNKNGNGV---STHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVN 315
           P E  KS    N NGNG     T  +LV+EIK++LKKGSS++ LKSFL SLSGTP+EI++
Sbjct: 245 PEEAAKSETKVNGNGNGTVTAETEDSLVDEIKEFLKKGSSSSQLKSFLKSLSGTPKEIMD 304

Query: 316 ALVEALFDGVGKGFSKEVMKKKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALV 375
           AL EALFDGVGKGF+KEV KKK++L AA   +GSQ+ LL+AIESFC     E  KEVALV
Sbjct: 305 ALFEALFDGVGKGFAKEVTKKKSYLVAATEEDGSQMVLLQAIESFCGKAGQEVTKEVALV 364

Query: 376 LKSLYDSNLIEEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           LK LYD++++EEEF++EWYQKG+ G NK+SPIW +VKPFIEWLQNAESE+EEE
Sbjct: 365 LKFLYDNDVLEEEFIMEWYQKGLAGGNKASPIWKNVKPFIEWLQNAESESEEE 417

BLAST of Cp4.1LG07g01200 vs. TrEMBL
Match: A0A022Q8M5_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a006876mg PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 8.2e-143
Identity = 276/426 (64.79%), Postives = 325/426 (76.29%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA N+DDAFYRYKMPRM+TKIEGRGNGIKTN+VNMV+IAKALGRPASYTTK+FGCELG
Sbjct: 5   NIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPASYTTKHFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTG S VNGAHDT+KLAGLLENFIKK+VQCYGCGNPETEIIITK+QMI +KCA
Sbjct: 65  AQSKFDEKTGASHVNGAHDTSKLAGLLENFIKKFVQCYGCGNPETEIIITKSQMINLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGFIS+VDMRDK+TTFILKNPPE KKGSKDKKAMRRAEKERLKEGE ADEE KKLKKD+
Sbjct: 125 ACGFISEVDMRDKLTTFILKNPPESKKGSKDKKAMRRAEKERLKEGEVADEEVKKLKKDA 184

Query: 196 VKKKSTSGNSKETVTKLSAVTADMVMLSTEE--TTSSKKPSEHEKSSINGNKNG------ 255
            KKK +SGN KE   K  A+T    + S E+     S    +HE    + + +       
Sbjct: 185 AKKKGSSGNLKEVAAK--AITKKKSIHSDEDHSPNGSSHADDHEVECDDDDDDNIQWQTD 244

Query: 256 --------------NGVSTHVALV------------------NEIKDYLKKGSSAANLKS 315
                         N ++  + ++                  NEIK ++KKGS  +  KS
Sbjct: 245 TSSLAAQQRIKEQLNAITASMVMLSTDEEKPKSGKISPKRATNEIKKFIKKGSPVSQFKS 304

Query: 316 FLGSLSGTPQEIVNALVEALFDGVGKGFSKEVMKKKNFLAAAVALEGSQIALLRAIESFC 375
            L SLSGT Q+I +AL EALF+GVGKGF+KEV+KK+N+LAAAV  EGSQ  LLRA+ESFC
Sbjct: 305 LLQSLSGTQQQITDALFEALFEGVGKGFAKEVVKKRNYLAAAVEEEGSQAVLLRAVESFC 364

Query: 376 LNTSSEAAKEVALVLKSLYDSNLIEEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNA 402
              S+EA KEVALVLK+LYDS+++EEEF++EWY+KGV G NK SPIW +VKPF+EWLQ+A
Sbjct: 365 GKASNEAVKEVALVLKALYDSDVVEEEFIVEWYKKGVNGGNKGSPIWKNVKPFVEWLQSA 424

BLAST of Cp4.1LG07g01200 vs. TrEMBL
Match: A0A151TD81_CAJCA (Eukaryotic translation initiation factor 5 OS=Cajanus cajan GN=KK1_019608 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 4.5e-141
Identity = 274/401 (68.33%), Postives = 317/401 (79.05%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA+N DDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFGCELG
Sbjct: 5   NIGAANSDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTGTS VNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITK QMI +KCA
Sbjct: 65  AQSKFDEKTGTSHVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKNQMIQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+TTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KKLKK+ 
Sbjct: 125 ACGFVSDVDMRDKLTTFIIKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKLKKEK 184

Query: 196 --------------VKKKSTSGNSKETVTKLSAVTADMVMLSTEETTSSKKPSEHEKSSI 255
                         +   S     +    +LSAVTADMVMLST+E    KK + +     
Sbjct: 185 EEAHDEDDDDDVQWLTDTSLDAARQRIQEQLSAVTADMVMLSTDEPEKKKKAASNGNG-- 244

Query: 256 NGNKNGNGVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVGK 315
            G++NGN +S +  LV E+K  LKKG  A  L S L +L+   QE ++ALVEALF+G  K
Sbjct: 245 -GSQNGNSMS-YGTLVGEVKANLKKGVGAKELMSHLAALTVPAQEKISALVEALFEGTEK 304

Query: 316 GFSKEVMKKKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLIEE 375
           GF+KE +KKKN+LAAAV  EGSQ+ LL A E F   ++S A KEVALVLK+LYD++++EE
Sbjct: 305 GFAKEAIKKKNYLAAAVGEEGSQLLLLNAFEEFSCKSTSNALKEVALVLKALYDADVLEE 364

Query: 376 EFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           EF+++WYQKG+ G NK S IW + +PFI+WLQNAESE+EEE
Sbjct: 365 EFIVQWYQKGLKGDNKKSQIWKNAQPFIDWLQNAESESEEE 401

BLAST of Cp4.1LG07g01200 vs. TrEMBL
Match: A0A151RII9_CAJCA (Eukaryotic translation initiation factor 5 OS=Cajanus cajan GN=KK1_036375 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 6.8e-137
Identity = 262/403 (65.01%), Postives = 321/403 (79.65%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA+N DDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKAL RP SYTTKYFGCELG
Sbjct: 5   NIGAANSDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPPSYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEK GTS VNGAHDTAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QM+ +KCA
Sbjct: 65  AQSKFDEKAGTSHVNGAHDTAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMVQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+T+FI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+KK+S
Sbjct: 125 ACGFVSDVDMRDKLTSFIIKNPPETKKGSKDKKAMRRAEKERLKEGEAADEEMKKIKKES 184

Query: 196 VKKKSTSGNSKET---------------VTKLSAVTADMVMLSTEETTSSKKPSEHEKSS 255
            KK++   +  E                  +LSAVTADMVMLST+E    +K +  +   
Sbjct: 185 KKKEAPDEDDDEVQWQTDTSLEAARQRIQEQLSAVTADMVMLSTDEPEKKEKGASKKS-- 244

Query: 256 INGNKNGNGVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVG 315
            +G++NGN +  +  LV E+K  L KG +A  L+S + +L    QE ++AL E LF+G  
Sbjct: 245 -DGSENGNSMH-YSKLVGEVKAILNKGIAANELQSHMAALPAPAQEKISALFEVLFEGTE 304

Query: 316 KGFSKEVMKKKNFLAAAVAL-EGSQIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLI 375
           KGF+KEV KKK +LAAAV++ EGSQ+ LLRAIE+FCL ++S A KE+A+VLK+LYD++++
Sbjct: 305 KGFAKEVAKKKRYLAAAVSVGEGSQLQLLRAIEAFCLKSTSNALKEIAIVLKALYDADVL 364

Query: 376 EEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           EEE++++W+Q+G+ G NK S IW + KP I+WLQ+AESE+EEE
Sbjct: 365 EEEYIVQWFQEGLKGDNKDSQIWKNAKPLIDWLQSAESESEEE 403

BLAST of Cp4.1LG07g01200 vs. TrEMBL
Match: K4BFW4_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.8e-134
Identity = 267/410 (65.12%), Postives = 315/410 (76.83%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASN DDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKAL RP SYTTKYFG ELG
Sbjct: 5   NIGASNSDDAFYRYKMPRMLTKIEGRGNGIKTNVVNMVDIAKALARPPSYTTKYFGNELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEK+GT+LVNGAH+T KLAGLLENFIKKYVQCYGCGNPETE+IITKTQMI +KCA
Sbjct: 65  AQSKFDEKSGTALVNGAHETPKLAGLLENFIKKYVQCYGCGNPETEVIITKTQMIQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGE---AADEEQKKLK 195
           ACGF+SD+DMRDK+TTFILKNPPE KKGSKDKKAMRRAEKERLKEGE    +DE+     
Sbjct: 125 ACGFLSDLDMRDKLTTFILKNPPEAKKGSKDKKAMRRAEKERLKEGEKHGGSDEDHASPP 184

Query: 196 KDSVKKK-----------------STSGNSKETVTKLSAVTADMVMLSTEETTSSKKPSE 255
           +  V  K                 S     +    +L+A TA+MVMLST ET   K  + 
Sbjct: 185 RSHVNVKEEEEEDDDDDVQWQTDTSLEAAQQRIQEQLNAATAEMVMLSTVETEKKKSKAP 244

Query: 256 HEKSSI---NGNKNGNGVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALV 315
             K++      +K  NGV+ H  LV EIK  L KG +A+ L+SFLGSLSG+PQE++ A  
Sbjct: 245 SPKAASVTPEISKTENGVTNHERLVREIKADLAKGVTASKLQSFLGSLSGSPQEVIAAFY 304

Query: 316 EALFDGVGKGFSKEVMKKKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALVLKS 375
           EAL DGV KGF+KEV+KKK++LAA    E SQ+ LL+AIE F + ++S A KEVALVLK+
Sbjct: 305 EALLDGVAKGFAKEVIKKKSYLAAIAQDEESQLRLLQAIEEFSVKSNSVALKEVALVLKA 364

Query: 376 LYDSNLIEEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           LYD +L+EEE++++WY KG VGANK S IW +VKPF+EWLQ+AESE+EEE
Sbjct: 365 LYDDDLLEEEYIVQWYNKG-VGANKDSKIWKNVKPFVEWLQSAESESEEE 413

BLAST of Cp4.1LG07g01200 vs. TAIR10
Match: AT1G36730.1 (AT1G36730.1 Translation initiation factor IF2/IF5)

HSP 1 Score: 340.1 bits (871), Expect = 1.8e-93
Identity = 171/198 (86.36%), Postives = 188/198 (94.95%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNRDDAFYRYKMPRM+TKIEGRGNGIKTNVVNMVEIAKALGRPA+YTTKYFGCELG
Sbjct: 5   NIGASNRDDAFYRYKMPRMMTKIEGRGNGIKTNVVNMVEIAKALGRPAAYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEK GTSLVNGAHDT+KLAGLLENFIKKYVQCYGCGNPETEI+ITKTQM+ +KCA
Sbjct: 65  AQSKFDEKNGTSLVNGAHDTSKLAGLLENFIKKYVQCYGCGNPETEILITKTQMLQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKD- 195
           ACGF+SDVDMRDK+T+FILKNPPEQKK SKDKK+MRRAEKERL+EGEAADEE +KLKK+ 
Sbjct: 125 ACGFLSDVDMRDKLTSFILKNPPEQKKSSKDKKSMRRAEKERLREGEAADEEMRKLKKEA 184

Query: 196 -SVKKKSTSGNSKETVTK 212
            S KK +T+G SK+ V+K
Sbjct: 185 ASKKKAATTGTSKDKVSK 202

BLAST of Cp4.1LG07g01200 vs. TAIR10
Match: AT1G77840.1 (AT1G77840.1 Translation initiation factor IF2/IF5)

HSP 1 Score: 318.2 bits (814), Expect = 7.5e-87
Identity = 162/195 (83.08%), Postives = 179/195 (91.79%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNR+DAFYRYKMP+MVTK EG+GNGIKTN++N VEIAKAL RP SYTTKYFGCELG
Sbjct: 5   NIGASNRNDAFYRYKMPKMVTKTEGKGNGIKTNIINNVEIAKALARPPSYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTGTSLVNGAH+T+KLAGLLENFIKK+VQCYGCGNPETEIIITKTQM+ +KCA
Sbjct: 65  AQSKFDEKTGTSLVNGAHNTSKLAGLLENFIKKFVQCYGCGNPETEIIITKTQMVNLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGFIS+VDMRDK+T FILKNPPEQKK SKDKKAMR+AEKERLKEGE ADEEQ+KLK   
Sbjct: 125 ACGFISEVDMRDKLTNFILKNPPEQKKVSKDKKAMRKAEKERLKEGELADEEQRKLK--- 184

Query: 196 VKKKSTSGNSKETVT 211
            KKK+ S N K++ T
Sbjct: 185 AKKKALS-NGKDSKT 195

BLAST of Cp4.1LG07g01200 vs. TAIR10
Match: AT5G20920.1 (AT5G20920.1 eukaryotic translation initiation factor 2 beta subunit)

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-07
Identity = 27/93 (29.03%), Postives = 45/93 (48.39%), Query Frame = 1

Query: 46  KTNVVNMVEIAKALGRPASYTTKYFGCELGAQSKFDEKTGTSLVNGAHDTAKLAGLLENF 105
           KT  VN +++ K + R   +  +Y   ELG     D +    +V G        G+L  +
Sbjct: 157 KTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRL-VVKGRFAPKNFEGILRRY 216

Query: 106 IKKYVQCYGCGNPETEIIITKTQMITMKCAACG 139
           I  YV C GC +P+T I+  + ++  ++C  CG
Sbjct: 217 ITDYVICLGCKSPDT-ILSKENRLFFLRCEKCG 247

BLAST of Cp4.1LG07g01200 vs. NCBI nr
Match: gi|629105069|gb|KCW70538.1| (hypothetical protein EUGRSUZ_F03737 [Eucalyptus grandis])

HSP 1 Score: 568.5 bits (1464), Expect = 9.0e-159
Identity = 306/413 (74.09%), Postives = 342/413 (82.81%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG
Sbjct: 5   NIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTG SLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMIT+KCA
Sbjct: 65  AQSKFDEKTGISLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+TTFILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEEQKKLKK++
Sbjct: 125 ACGFLSDVDMRDKLTTFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEEQKKLKKEA 184

Query: 196 VKKKSTSGN---SKETVTK-----------------LSAVTADMVMLSTEETTSS---KK 255
            KKK+++ +   SK   TK                 LSA TADMVMLST E  S    K 
Sbjct: 185 AKKKASATSKDASKGASTKKKNLSDEEHSPPGSQEQLSAATADMVMLSTNEEKSKSTKKL 244

Query: 256 PSEHEKSSINGNKNGNGV---STHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVN 315
           P E  KS    N NGNG     T  +LV+EIK++LKKGSS++ LKSFL SLSGTP+EI++
Sbjct: 245 PEEAAKSETKVNGNGNGTVTAETEDSLVDEIKEFLKKGSSSSQLKSFLKSLSGTPKEIMD 304

Query: 316 ALVEALFDGVGKGFSKEVMKKKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALV 375
           AL EALFDGVGKGF+KEV KKK++L AA   +GSQ+ LL+AIESFC     E  KEVALV
Sbjct: 305 ALFEALFDGVGKGFAKEVTKKKSYLVAATEEDGSQMVLLQAIESFCGKAGQEVTKEVALV 364

Query: 376 LKSLYDSNLIEEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           LK LYD++++EEEF++EWYQKG+ G NK+SPIW +VKPFIEWLQNAESE+EEE
Sbjct: 365 LKFLYDNDVLEEEFIMEWYQKGLAGGNKASPIWKNVKPFIEWLQNAESESEEE 417

BLAST of Cp4.1LG07g01200 vs. NCBI nr
Match: gi|848913851|ref|XP_012854941.1| (PREDICTED: eukaryotic translation initiation factor 5 [Erythranthe guttata])

HSP 1 Score: 515.0 bits (1325), Expect = 1.2e-142
Identity = 276/426 (64.79%), Postives = 325/426 (76.29%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA N+DDAFYRYKMPRM+TKIEGRGNGIKTN+VNMV+IAKALGRPASYTTK+FGCELG
Sbjct: 5   NIGAGNKDDAFYRYKMPRMITKIEGRGNGIKTNIVNMVDIAKALGRPASYTTKHFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTG S VNGAHDT+KLAGLLENFIKK+VQCYGCGNPETEIIITK+QMI +KCA
Sbjct: 65  AQSKFDEKTGASHVNGAHDTSKLAGLLENFIKKFVQCYGCGNPETEIIITKSQMINLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGFIS+VDMRDK+TTFILKNPPE KKGSKDKKAMRRAEKERLKEGE ADEE KKLKKD+
Sbjct: 125 ACGFISEVDMRDKLTTFILKNPPESKKGSKDKKAMRRAEKERLKEGEVADEEVKKLKKDA 184

Query: 196 VKKKSTSGNSKETVTKLSAVTADMVMLSTEE--TTSSKKPSEHEKSSINGNKNG------ 255
            KKK +SGN KE   K  A+T    + S E+     S    +HE    + + +       
Sbjct: 185 AKKKGSSGNLKEVAAK--AITKKKSIHSDEDHSPNGSSHADDHEVECDDDDDDNIQWQTD 244

Query: 256 --------------NGVSTHVALV------------------NEIKDYLKKGSSAANLKS 315
                         N ++  + ++                  NEIK ++KKGS  +  KS
Sbjct: 245 TSSLAAQQRIKEQLNAITASMVMLSTDEEKPKSGKISPKRATNEIKKFIKKGSPVSQFKS 304

Query: 316 FLGSLSGTPQEIVNALVEALFDGVGKGFSKEVMKKKNFLAAAVALEGSQIALLRAIESFC 375
            L SLSGT Q+I +AL EALF+GVGKGF+KEV+KK+N+LAAAV  EGSQ  LLRA+ESFC
Sbjct: 305 LLQSLSGTQQQITDALFEALFEGVGKGFAKEVVKKRNYLAAAVEEEGSQAVLLRAVESFC 364

Query: 376 LNTSSEAAKEVALVLKSLYDSNLIEEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNA 402
              S+EA KEVALVLK+LYDS+++EEEF++EWY+KGV G NK SPIW +VKPF+EWLQ+A
Sbjct: 365 GKASNEAVKEVALVLKALYDSDVVEEEFIVEWYKKGVNGGNKGSPIWKNVKPFVEWLQSA 424

BLAST of Cp4.1LG07g01200 vs. NCBI nr
Match: gi|1012353806|gb|KYP64994.1| (Eukaryotic translation initiation factor 5 [Cajanus cajan])

HSP 1 Score: 509.2 bits (1310), Expect = 6.5e-141
Identity = 274/401 (68.33%), Postives = 317/401 (79.05%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA+N DDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKAL RPASYTTKYFGCELG
Sbjct: 5   NIGAANSDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTGTS VNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITK QMI +KCA
Sbjct: 65  AQSKFDEKTGTSHVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKNQMIQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+TTFI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KKLKK+ 
Sbjct: 125 ACGFVSDVDMRDKLTTFIIKNPPEVKKGSKDKKAMRRAEKERLKEGEAADEELKKLKKEK 184

Query: 196 --------------VKKKSTSGNSKETVTKLSAVTADMVMLSTEETTSSKKPSEHEKSSI 255
                         +   S     +    +LSAVTADMVMLST+E    KK + +     
Sbjct: 185 EEAHDEDDDDDVQWLTDTSLDAARQRIQEQLSAVTADMVMLSTDEPEKKKKAASNGNG-- 244

Query: 256 NGNKNGNGVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVGK 315
            G++NGN +S +  LV E+K  LKKG  A  L S L +L+   QE ++ALVEALF+G  K
Sbjct: 245 -GSQNGNSMS-YGTLVGEVKANLKKGVGAKELMSHLAALTVPAQEKISALVEALFEGTEK 304

Query: 316 GFSKEVMKKKNFLAAAVALEGSQIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLIEE 375
           GF+KE +KKKN+LAAAV  EGSQ+ LL A E F   ++S A KEVALVLK+LYD++++EE
Sbjct: 305 GFAKEAIKKKNYLAAAVGEEGSQLLLLNAFEEFSCKSTSNALKEVALVLKALYDADVLEE 364

Query: 376 EFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           EF+++WYQKG+ G NK S IW + +PFI+WLQNAESE+EEE
Sbjct: 365 EFIVQWYQKGLKGDNKKSQIWKNAQPFIDWLQNAESESEEE 401

BLAST of Cp4.1LG07g01200 vs. NCBI nr
Match: gi|1009136567|ref|XP_015885593.1| (PREDICTED: probable eukaryotic translation initiation factor 5-1 [Ziziphus jujuba])

HSP 1 Score: 507.3 bits (1305), Expect = 2.5e-140
Identity = 288/437 (65.90%), Postives = 322/437 (73.68%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGASNRDDAFYRYKMP+M+TKIEGRGNGIKTNVVNMVEIAKAL RPASYTTKYFGCELG
Sbjct: 5   NIGASNRDDAFYRYKMPKMITKIEGRGNGIKTNVVNMVEIAKALARPASYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEKTGT+LVNGAHDTAKLA LLENFIKKYVQCYGCGNPETEIIITKTQMIT+KCA
Sbjct: 65  AQSKFDEKTGTALVNGAHDTAKLAALLENFIKKYVQCYGCGNPETEIIITKTQMITLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGFISDVDMRDK+T+FILKNPPEQKK SKDKKAMRRAEKERLKEGEAADEE KKLKK++
Sbjct: 125 ACGFISDVDMRDKLTSFILKNPPEQKKSSKDKKAMRRAEKERLKEGEAADEELKKLKKEA 184

Query: 196 VKKKSTSGNSKETVTKLS-------------------AVTADMVMLSTEETTSSKKPSEH 255
           +KKK  SG+SK +V K S                   A   D   +  +  TS +   + 
Sbjct: 185 IKKKGASGSSKSSVKKKSNGSDEDHSPSHSQIDENDHAANDDEDDVQWQTDTSLEAAQKR 244

Query: 256 EKSSING-NKNGNGVSTHV-------------------------------ALVNEIKDYL 315
            +  +N        +STH                                 LV EIK+ L
Sbjct: 245 IQEQLNAVTSEMVMLSTHEDKQKSSKKSPEHEQKSEINGHENGALDSNQGKLVGEIKECL 304

Query: 316 KKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVGKGFSKEVMKKKNFLAAAVALEG-S 375
           KKGSS +  KSF+GSLSGT QEI+NAL EALFDGVGKGF+KEV KKKN+L  AVA EG S
Sbjct: 305 KKGSSISQFKSFVGSLSGTSQEIMNALFEALFDGVGKGFAKEVAKKKNYL-EAVAREGES 364

Query: 376 QIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLIEEEFVLEWYQKGVVGANKSSPIWN 401
           Q+ LL AIE F    S EA KEV LVLK+LYD +L+EEEF++EWY +G VG+NKSS IW 
Sbjct: 365 QMLLLHAIEYFSAKASPEAVKEVPLVLKALYDCDLLEEEFIMEWYNQGSVGSNKSSQIWK 424

BLAST of Cp4.1LG07g01200 vs. NCBI nr
Match: gi|1012330727|gb|KYP42235.1| (Eukaryotic translation initiation factor 5 [Cajanus cajan])

HSP 1 Score: 495.4 bits (1274), Expect = 9.7e-137
Identity = 262/403 (65.01%), Postives = 321/403 (79.65%), Query Frame = 1

Query: 16  NIGASNRDDAFYRYKMPRMVTKIEGRGNGIKTNVVNMVEIAKALGRPASYTTKYFGCELG 75
           NIGA+N DDAFYRYKMPRM+TKIEGRGNGIKTNVVNMV+IAKAL RP SYTTKYFGCELG
Sbjct: 5   NIGAANSDDAFYRYKMPRMITKIEGRGNGIKTNVVNMVDIAKALARPPSYTTKYFGCELG 64

Query: 76  AQSKFDEKTGTSLVNGAHDTAKLAGLLENFIKKYVQCYGCGNPETEIIITKTQMITMKCA 135
           AQSKFDEK GTS VNGAHDTAKLAGLLE FIKKYVQCYGCGNPETEI+ITK QM+ +KCA
Sbjct: 65  AQSKFDEKAGTSHVNGAHDTAKLAGLLEIFIKKYVQCYGCGNPETEILITKNQMVQLKCA 124

Query: 136 ACGFISDVDMRDKITTFILKNPPEQKKGSKDKKAMRRAEKERLKEGEAADEEQKKLKKDS 195
           ACGF+SDVDMRDK+T+FI+KNPPE KKGSKDKKAMRRAEKERLKEGEAADEE KK+KK+S
Sbjct: 125 ACGFVSDVDMRDKLTSFIIKNPPETKKGSKDKKAMRRAEKERLKEGEAADEEMKKIKKES 184

Query: 196 VKKKSTSGNSKET---------------VTKLSAVTADMVMLSTEETTSSKKPSEHEKSS 255
            KK++   +  E                  +LSAVTADMVMLST+E    +K +  +   
Sbjct: 185 KKKEAPDEDDDEVQWQTDTSLEAARQRIQEQLSAVTADMVMLSTDEPEKKEKGASKKS-- 244

Query: 256 INGNKNGNGVSTHVALVNEIKDYLKKGSSAANLKSFLGSLSGTPQEIVNALVEALFDGVG 315
            +G++NGN +  +  LV E+K  L KG +A  L+S + +L    QE ++AL E LF+G  
Sbjct: 245 -DGSENGNSMH-YSKLVGEVKAILNKGIAANELQSHMAALPAPAQEKISALFEVLFEGTE 304

Query: 316 KGFSKEVMKKKNFLAAAVAL-EGSQIALLRAIESFCLNTSSEAAKEVALVLKSLYDSNLI 375
           KGF+KEV KKK +LAAAV++ EGSQ+ LLRAIE+FCL ++S A KE+A+VLK+LYD++++
Sbjct: 305 KGFAKEVAKKKRYLAAAVSVGEGSQLQLLRAIEAFCLKSTSNALKEIAIVLKALYDADVL 364

Query: 376 EEEFVLEWYQKGVVGANKSSPIWNHVKPFIEWLQNAESETEEE 403
           EEE++++W+Q+G+ G NK S IW + KP I+WLQ+AESE+EEE
Sbjct: 365 EEEYIVQWFQEGLKGDNKDSQIWKNAKPLIDWLQSAESESEEE 403

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
IF5Y_ARATH3.3e-9286.36Probable eukaryotic translation initiation factor 5-1 OS=Arabidopsis thaliana GN... [more]
IF5_MAIZE4.3e-9263.97Eukaryotic translation initiation factor 5 OS=Zea mays GN=EIF5 PE=2 SV=1[more]
IF5_PHAVU5.4e-8773.11Eukaryotic translation initiation factor 5 OS=Phaseolus vulgaris GN=EIF5 PE=2 SV... [more]
IF5Z_ARATH1.3e-8583.08Probable eukaryotic translation initiation factor 5-2 OS=Arabidopsis thaliana GN... [more]
IF5_MOUSE2.7e-4634.06Eukaryotic translation initiation factor 5 OS=Mus musculus GN=Eif5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A059BWC9_EUCGR6.3e-15974.09Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F03737 PE=4 SV=1[more]
A0A022Q8M5_ERYGU8.2e-14364.79Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a006876mg PE=4 SV=1[more]
A0A151TD81_CAJCA4.5e-14168.33Eukaryotic translation initiation factor 5 OS=Cajanus cajan GN=KK1_019608 PE=4 S... [more]
A0A151RII9_CAJCA6.8e-13765.01Eukaryotic translation initiation factor 5 OS=Cajanus cajan GN=KK1_036375 PE=4 S... [more]
K4BFW4_SOLLC1.8e-13465.12Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G36730.11.8e-9386.36 Translation initiation factor IF2/IF5[more]
AT1G77840.17.5e-8783.08 Translation initiation factor IF2/IF5[more]
AT5G20920.12.6e-0729.03 eukaryotic translation initiation factor 2 beta subunit[more]
Match NameE-valueIdentityDescription
gi|629105069|gb|KCW70538.1|9.0e-15974.09hypothetical protein EUGRSUZ_F03737 [Eucalyptus grandis][more]
gi|848913851|ref|XP_012854941.1|1.2e-14264.79PREDICTED: eukaryotic translation initiation factor 5 [Erythranthe guttata][more]
gi|1012353806|gb|KYP64994.1|6.5e-14168.33Eukaryotic translation initiation factor 5 [Cajanus cajan][more]
gi|1009136567|ref|XP_015885593.1|2.5e-14065.90PREDICTED: probable eukaryotic translation initiation factor 5-1 [Ziziphus jujub... [more]
gi|1012330727|gb|KYP42235.1|9.7e-13765.01Eukaryotic translation initiation factor 5 [Cajanus cajan][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005488binding
GO:0005515protein binding
GO:0003743translation initiation factor activity
Vocabulary: Biological Process
TermDefinition
GO:0006413translational initiation
Vocabulary: INTERPRO
TermDefinition
IPR016190Transl_init_fac_IF2/IF5_Zn-bd
IPR016189Transl_init_fac_IF2/IF5_N
IPR016024ARM-type_fold
IPR016021MIF4-like_sf
IPR003307W2_domain
IPR002735Transl_init_fac_IF2/IF5
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006446 regulation of translational initiation
biological_process GO:0006413 translational initiation
cellular_component GO:0005840 ribosome
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0003743 translation initiation factor activity
molecular_function GO:0005488 binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g01200.1Cp4.1LG07g01200.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002735Translation initiation factor IF2/IF5PFAMPF01873eIF-5_eIF-2Bcoord: 22..138
score: 3.3
IPR002735Translation initiation factor IF2/IF5SMARTSM00653eIF2Bneu4coord: 26..140
score: 4.1
IPR003307W2 domainPFAMPF02020W2coord: 324..402
score: 8.9
IPR003307W2 domainSMARTSM00515542_3coord: 311..398
score: 2.2
IPR003307W2 domainPROFILEPS51363W2coord: 246..402
score: 23
IPR016021MIF4G-like domainGENE3DG3DSA:1.25.40.180coord: 256..402
score: 3.0
IPR016024Armadillo-type foldunknownSSF48371ARM repeatcoord: 257..396
score: 2.68
IPR016189Translation initiation factor IF2/IF5, N-terminalGENE3DG3DSA:3.30.30.50coord: 41..83
score: 3.2
IPR016189Translation initiation factor IF2/IF5, N-terminalunknownSSF100966Translation initiation factor 2 beta, aIF2beta, N-terminal domaincoord: 20..112
score: 8.63
IPR016190Translation initiation factor IF2/IF5, zinc-bindingunknownSSF75689Zinc-binding domain of translation initiation factor 2 betacoord: 109..144
score: 1.8
NoneNo IPR availableunknownCoilCoilcoord: 170..190
scor
NoneNo IPR availablePANTHERPTHR23001EUKARYOTIC TRANSLATION INITIATION FACTORcoord: 16..268
score: 2.6E-221coord: 285..402
score: 2.6E
NoneNo IPR availablePANTHERPTHR23001:SF8EUKARYOTIC TRANSLATION INITIATION FACTOR 5-2-RELATEDcoord: 16..268
score: 2.6E-221coord: 285..402
score: 2.6E