Lsi07G008980 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi07G008980
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Transcription initiation factor IIE subunit beta
Location	chr07 : 10262891 .. 10268227 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAGAATTGGTTGGGTTTCTTCATGGACCGACGCCATATCTCATTTTAACCTTCCTCAAAGAAGTCGTCCCCCATCTCTCTCTCTCTTTCCCCCTCGCGCTTTCCCAATTCCAGCTTCCATCTTCCATCTTCCATCTTCATTCTTCTCGACCTTCATAGCCGCACGAACTGAACTCGATTCAACAATCAACTCCATTACGTATACTAAAAGTTATCGACCAGCGATCCCACCGTCGTCTTATCCACGGCGGTCAATTCTGGTCTGTCTAACCGATGCAATTTGTGGTATTCTACTAACTATCCTTTTCTTTTCTGGGTATTTTCAATCGTGGGTTGGGCTTGATGGGATGCTCGGCAATTTGATTTTATTGGGTGAATCTATTTTGATGATTTTTATTGTTATTTTGATTGTTAGGGTTGAGAATCATTGAAAACTGTGAGGGGATTCAATGGCGTTGCAAGAACAACTAGATAGGTTTAAGAAACAGCAAGAAAGATGTCAGACTACTTTGTCTGGCATTGCTGCTTCCAAGTCTGCATCCAAGCCTACACCCAAGTTTACGCCTGCACCAGCTGCTTCCATTGCAAGACCTCCGGCTCCTGCTGTGAAATTTTCAAATGATACTGAGAGACTTCAACACATTAACAGCATAAGGAAATCCCCAGTCGGGGCTCAGATCAAGAGGGTTATTGACCTGCTGCTGGAGGTTCACACCCATCACCCATCTTTCTTATTTTTTCCCCTTTTGTTTTCTTCCTTCCACCTTATTTGCTTTACTTGGTTTCTTGTGTTTGATCAAATTCTTCAATGTAAGGCTTTGTATAGGACAGGATTTAATGAACCGTTTATTGATTTGAACTGTGTAGAGCATATGCTGAAATTTCGCGTTATTAATCTGTTCAAGTTCCATAAATTCAATATGTGTTGTTTCTAATGGATACTCTTTTAACCGGGAGATTTGGCGGGCATGGGGGCTTCTTCAGGTAGTGAGACTTGTGATTGGCATTAGATGGCAAGAGGAGCTAGGATCCTCTTCAGTGGCTTTTGTGTGGTCGTTTTCCTTACTTTTTGTTGGGGAAAAAAATGTATTTTACAGGCAAATTACATATGTTAATACCCTCCCCGTGCTCTCTGGATTTTGAATTGGCTGTGAAAAAGCTAAAGAAAAGTCCATCTGAAATGCATTTTTCTCTATTCGTAGTAAATCAGAACTGTAGGGTTATGCTACAATCTTATAGCTTGCTTATTGTGCATGTTAATAGACAAGGCAAGCCTTTACACCTGAGCAAATCAATGAAGCATGTTATGTTGATATAAATTCAAACAAGGCTGTCTATGATAGTCTGCGGAATAACCCAAAGGTGCACTATGATGGGAGGCGTTTCTCATACAAGGTAATTTATATTCTCAAACACCTGTATAATTTGCATCGACTTGTACTTGTTTAGTTTAAAAGTTTGAACCTTCAGGATGACATTTTTTCCCCTCTTTTTTCTGTTTTTGGTGTATTCAGTCCAAGCATGACCTGAAGGATAAGAGTCAACTTCTTTACTTGATTAGAAAATTTCCAGAAGGCATTGCTGTCATTGATCTCAAGGATGCTTATCCAAGCGTTATGGAGGACTTGCAAGTACGTATAATAAAGAATTTTTTAATGTGATTTTCCCATTTTGAATGCGACAAAGTGCAAAATAACAAATGACCCTGGAATGATGCCTAAGAAGCACAGACACTTTAGTTTGGCTAGCGTGTCCGTGTCCGTGTCCGACACGTGTCGGACACTTGGACACTCCGACACTTGTTGAACACGTATCGGACACTTGTTAGTGCAACAAATGTGTTAGACATGCATTGAACACTTGTTGAGTAGACCAAAAAGACACATATATGACAATATGACAATAATACTAACTTTTGAGTGTGAAATACATCAAGCTAAGTCTTTTAAGCATATAAATGCATCAACCCATTTACTTTTAATTTTATTTTGGTATGAAAATGATATATATTTTCAAAAATGTATATTTTAGTAAACGTGTCATTGCCGTGTCATGTCCTAGATATTTGAAATATGGCGTGTCGCCGTGTCCGTGTCGTGTCATATCCGTGTCTCGTATCCGTGTCCGTGCTTCTTAGAATGATGCTATCCTTATTTGAACTGCTTGGGCAAAATAACCTTTGTGATGTTCCAACATATTGGCTATTTTGGACATCAAATGTCTTGTGTAATTCTTGAGCTTTTAAGGTCGTGTATTTTATTTGCACACGGATCAGAATTATAGTTCATTTATATTTCTAATTACCTCAGAGATTAGTACAAGAGTTTGTCGGGATCTGTATTCTAATTTCTATATGGATATAAATGCAGTGTAAATTAAAGCCGGCAAGTTATTAAGTTTTGATTTGTCTATTAAAGGGGTAGTTTGAGAATGTTGTGAGAAGCATCTTTCTCTGATAGCTGGTGAATGGTGATTTTATGTGGAGAATAGCCTCAGATAATCAACCATATGGTCTGAAAACTGTTGTTAAAAATATTTTTGAAAATAAAAGGAAAGATTTGTTCTAAGTAGCTGTGAGAAAAGCCCCCAATTTCCTTTGCTAGTATACCCCTTGTGTTTCCTCCCTGTTTTTCCTCCTCTCCATCCGATAAGAAGCATGGGCACTTTAGTTTGACTAGCGTTCGACACTCTGACACTTGTGGGACACGTATCAGACACTTGTTAGTATAATAGTTGTTTAGACACTAGTTGTATGAAGTCAAAATAGAACCAACATTTGTTAGGCATGTATCAAACACTTGTTATATATACTAAATAAATACATTAAGACAAAAATAATAAATTTTGATAATGAAATACATCAAACTAATTTTTTAAGCATATAAATGAATAAATTCATTGACTTTAAATTTCCTTCTGGTTTAAAAATGATATATATTTTAAAAAAAATGTATGTTTTAATGAATACGTCATTGTCGTGTCCGTGTCTTAAATTTTTAAAAAATGTCGTGTCATCGTGTCTGGGTAGTGACGATCCATGCCTTGTATCTGTATCCACGCTTCTTAGCCATCCCCCATTTTAGGAGTTAGTACCAGACTGATTTTAGAGCAAGCTCGTTCCTACCTGCAGAAATTTGATTGATGCCTGTATGACTTGCTCACTATTCATGTTGACGTTCAAGCCTTCAAGGTTCTTGTTCATGCTATACCTCATAAGTCAGTCTGTTTACTTTTCTTCCTGGTTAGACTGTGGTGTCTCCTTTGTACATTAGAAGGTTTTATTCATCCAACAATTCCTATCCCAAAAGTCGTAGAGAATAGTGTTAATGATTAGGAGCTGAAATTTAAATTGAAGGATTTAACCCCATGATTTAGTCATTGTCAAAACAAATTGTTTTTCTAATGAGGAGCCAAACTTCCATTGAGAAACATGAAATAGTTTAGAAGTGGAAACAAAAGGACAATTCAACAACTGGAGCCAATCCGAGACTAACCATACGAACGGGCTCAAATCTAAAATTATAAGGTCTAACAAATAATTACAATTCTTGGAAACCAATGTACATTGTACAAGAAGAAGCATTGAACTTTGTTGCACCTAAATTGTAGTTGAGAATTTGCAAAAATCTACAAGAAACGTTTGCTTTTGGCAATAACCTATACATTAAGCTTGAAAAAGAACGGGTGGTAGGGCCTAAACATCCAAGTCATCCACGACTTCAATTCCTTCAAGTAATCTTGGTAACCATGATAAAATAATTGAGGCCAGTACGAAGATGAAGAACCTCTCTTTCTCTCTTTGGATAGAAACAACTGCTGTCATTGAGGAAAAAAAATGAAAGGAGGGCTTACAAAAAAACCTCCTAAAGAAAGGGTTTTCAACTAGGCAAGATGTCCATATTTATTATATAAAAATAACATAATATCTCATAGGTTAGGAATTTTACTTATAATTATGCTTTTGGGTTATGCATCTAATTTAATAAAGATATGTGGAGTGATTCGTGGGAATTTTACATTGAACTTAGTGCTCCTGTACTTCTTTTATTTTATTTTATTTTTGGTAATGACATGATATTCAGCTGCAGTAGGATGACCGGCTTGGAGGTTATGTTTCTTGAGAAGTGATGTCTATCTATTTCCATACCGTGTTGATTGCCCTTTTTTTGGGCTCCCAAACTATATAAAGTTCTCTTTGACGGAACCGGCCCTTGTATTGTTACAACTAATTTGCTTATATAATGAATGGAAAGCACATGGCTGTACTCTTCTTTGACTATATAATGAATGGAAAACACAGTCCATTAGCTTTACTTTTTGAATTACGTTATATTTATTTCCCCCTCTTGTTACAACTAATTTGCTTATATAATGAAAGGAAAGCACATGGCTGTACTCTTCTTTGACTATATAATAAATGGAAAACACAGTCCATTAGCTTTACTTTTTGAATTACGTTATATTTATTTCCCCCTCTTGTTACAACTAATTTGCTTATATAATGAAAGGAAAGCACATGGCTGTACTCTTCTTTGACTATATAATAAATGGAAAACACAGTCCATTAGCTTTACTTTTTGAATTACGTTATATTTATTTCCCCCTCTTGTTACAACTAATTTGCTTATATAATGAATGGAAAGCACATGGCTGTACTCTTCTTTGACTATATAATGAATGGAAAACACAGTCCATTAGCTTTACTTTTTGAATTACGTTATATTTATTTCCCCCTCTTGTTTAGGCCCTAAAAGCCTCAGGCCAGGTTTGGCTTCTCTCCAACTTCGATTCACAAGAAGATATTGCATATCCAAACGACCCTCGAATTCAAATCAAGGTAGACGATGATCTGAAGCAACTCTTTCGGGAGATTGAATTACCTCGTGACATGCTTGATGTTGAAAAGGATCTGCAGAAGAATGGGATGAAGCCTGCAACCAACACAGCGAAGAGGCGAGCCAATGCACAACATGTAGCATCCTCGAAACCGAAGCCAAAGAAAAAGAAGCAAGAGATCAGCAAGAGGACTAAGCTCACCAATGCACATCTTCCCGAGCTCTTCAAAAACTAGAGAACCATATAGGCTGGGATGCAACAATCTTTGTTATATGGATAGTTCATGGACTCTGTATATAGGATTCAACTATCCCTTCTGTTTCGTGTATTGCCTTTTCTTTCCCATGATATCATCATAGAATGAAATGCTCCTGTGGTTTACTTTTGTGTATGTCTTTGATTTTACATCAATTAATTGAACAAATTGAAATGTAAGATACAGGTTCAATTAAACTAGATAAATGATTTTTGGGAGATGAAATTATGGCATTTGCTTGCTTC

mRNA sequence

CGAGAATTGGTTGGGTTTCTTCATGGACCGACGCCATATCTCATTTTAACCTTCCTCAAAGAAGTCGTCCCCCATCTCTCTCTCTCTTTCCCCCTCGCGCTTTCCCAATTCCAGCTTCCATCTTCCATCTTCCATCTTCATTCTTCTCGACCTTCATAGCCGCACGAACTGAACTCGATTCAACAATCAACTCCATTACGTATACTAAAAGTTATCGACCAGCGATCCCACCGTCGTCTTATCCACGGCGGTCAATTCTGGTCTGTCTAACCGATGCAATTTGTGGGTTGAGAATCATTGAAAACTGTGAGGGGATTCAATGGCGTTGCAAGAACAACTAGATAGGTTTAAGAAACAGCAAGAAAGATGTCAGACTACTTTGTCTGGCATTGCTGCTTCCAAGTCTGCATCCAAGCCTACACCCAAGTTTACGCCTGCACCAGCTGCTTCCATTGCAAGACCTCCGGCTCCTGCTGTGAAATTTTCAAATGATACTGAGAGACTTCAACACATTAACAGCATAAGGAAATCCCCAGTCGGGGCTCAGATCAAGAGGGTTATTGACCTGCTGCTGGAGACAAGGCAAGCCTTTACACCTGAGCAAATCAATGAAGCATGTTATGTTGATATAAATTCAAACAAGGCTGTCTATGATAGTCTGCGGAATAACCCAAAGGTGCACTATGATGGGAGGCGTTTCTCATACAAGTCCAAGCATGACCTGAAGGATAAGAGTCAACTTCTTTACTTGATTAGAAAATTTCCAGAAGGCATTGCTGTCATTGATCTCAAGGATGCTTATCCAAGCGTTATGGAGGACTTGCAAGCCCTAAAAGCCTCAGGCCAGGTTTGGCTTCTCTCCAACTTCGATTCACAAGAAGATATTGCATATCCAAACGACCCTCGAATTCAAATCAAGGTAGACGATGATCTGAAGCAACTCTTTCGGGAGATTGAATTACCTCGTGACATGCTTGATGTTGAAAAGGATCTGCAGAAGAATGGGATGAAGCCTGCAACCAACACAGCGAAGAGGCGAGCCAATGCACAACATGTAGCATCCTCGAAACCGAAGCCAAAGAAAAAGAAGCAAGAGATCAGCAAGAGGACTAAGCTCACCAATGCACATCTTCCCGAGCTCTTCAAAAACTAGAGAACCATATAGGCTGGGATGCAACAATCTTTGTTATATGGATAGTTCATGGACTCTGTATATAGGATTCAACTATCCCTTCTGTTTCGTGTATTGCCTTTTCTTTCCCATGATATCATCATAGAATGAAATGCTCCTGTGGTTTACTTTTGTGTATGTCTTTGATTTTACATCAATTAATTGAACAAATTGAAATGTAAGATACAGGTTCAATTAAACTAGATAAATGATTTTTGGGAGATGAAATTATGGCATTTGCTTGCTTC

Coding sequence (CDS)

ATGGCGTTGCAAGAACAACTAGATAGGTTTAAGAAACAGCAAGAAAGATGTCAGACTACTTTGTCTGGCATTGCTGCTTCCAAGTCTGCATCCAAGCCTACACCCAAGTTTACGCCTGCACCAGCTGCTTCCATTGCAAGACCTCCGGCTCCTGCTGTGAAATTTTCAAATGATACTGAGAGACTTCAACACATTAACAGCATAAGGAAATCCCCAGTCGGGGCTCAGATCAAGAGGGTTATTGACCTGCTGCTGGAGACAAGGCAAGCCTTTACACCTGAGCAAATCAATGAAGCATGTTATGTTGATATAAATTCAAACAAGGCTGTCTATGATAGTCTGCGGAATAACCCAAAGGTGCACTATGATGGGAGGCGTTTCTCATACAAGTCCAAGCATGACCTGAAGGATAAGAGTCAACTTCTTTACTTGATTAGAAAATTTCCAGAAGGCATTGCTGTCATTGATCTCAAGGATGCTTATCCAAGCGTTATGGAGGACTTGCAAGCCCTAAAAGCCTCAGGCCAGGTTTGGCTTCTCTCCAACTTCGATTCACAAGAAGATATTGCATATCCAAACGACCCTCGAATTCAAATCAAGGTAGACGATGATCTGAAGCAACTCTTTCGGGAGATTGAATTACCTCGTGACATGCTTGATGTTGAAAAGGATCTGCAGAAGAATGGGATGAAGCCTGCAACCAACACAGCGAAGAGGCGAGCCAATGCACAACATGTAGCATCCTCGAAACCGAAGCCAAAGAAAAAGAAGCAAGAGATCAGCAAGAGGACTAAGCTCACCAATGCACATCTTCCCGAGCTCTTCAAAAACTAG

Protein sequence

MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKVHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRRANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN

Homology

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BLAST of Lsi07G008980 vs. Swiss-Prot
Match: T2EB_DICDI (General transcription factor IIE subunit 2 OS=Dictyostelium discoideum GN=gtf2e2 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.2e-14
Identity = 73/275 (26.55%), Postives = 142/275 (51.64%), Query Frame = 1


Query: 10  FKKQQERCQTTLSGI----AASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTERLQHI 69
           FK+ Q +   TL+ I    +++ SA K  PK +    +S A   +     S  T      
Sbjct: 6   FKETQMKAIETLNSIKKPSSSTNSAFKYIPKISGGGGSSSANRQS-----SIYTANTLRD 65

Query: 70  NSIRKSPVGAQIKRVIDLL--LETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKVHYD 129
             +++ P    +  +++ L  LE   A T E ++   ++ I+    + + LRNN K+   
Sbjct: 66  KQVKEKPSNRIVYDIVNYLKTLEGIPADTKEIMHSTNHM-IDDKPEILELLRNNEKIIDH 125

Query: 130 GR-RFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLLSN 189
           G  RFS+K K +++ +  +L L+  +P GI V +L ++Y +   D++ LK + Q++ +  
Sbjct: 126 GNDRFSFKPKFNVRTQRDILDLLPNYPGGILVSELAESYNNAESDVKKLKETKQIFAIKA 185

Query: 190 FDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRRAN 249
            +S  DI +PND R+++ +  +L  +++ I+LP +  D+EK+++  G+    +  +   +
Sbjct: 186 AESACDIIFPNDERLRVPLSSELVDMWKSIKLPNE-ADLEKEMKDAGL----SVVESVES 245

Query: 250 AQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           A+   S+K K ++KK    + TK+TN H+     N
Sbjct: 246 ARQTKSTKLKKERKK----RITKVTNTHIENFNPN 265

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BLAST of Lsi07G008980 vs. Swiss-Prot
Match: T2EB_SCHPO (Transcription initiation factor IIE subunit beta OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tfa2 PE=1 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-11
Identity = 80/294 (27.21%), Postives = 143/294 (48.64%), Query Frame = 1


Query: 2   ALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPK--FTPAPAASI----------ARPP 61
           +L +QL  FKK+          +A     +KP P+   +P P A +          + P 
Sbjct: 3   SLSDQLSSFKKK----------VANQPIYAKPQPRQPASPTPTAYLNSNDGHSSAASSPG 62

Query: 62  APAVKFSNDTERLQHINSIRKSPVG----AQIKRVIDLLLETRQAFTPEQINEACYVDIN 121
           + ++K       L + +    S VG    +Q+   ++ L E  +  T E+I  A Y+   
Sbjct: 63  SYSLKKKRSKTSLVY-SQPADSGVGTHYLSQLHYAVEYLKERNEPKTAEEI--ASYLSTP 122

Query: 122 SNKAVYDSLRNNPKVHYDGRR--FSYKSKHDLKDKSQLLYLI--RKFPEGIAVIDLKDAY 181
               + + L+ N +++YD R   F++K  H+++  + LL  +  +K   G+++ +L+D +
Sbjct: 123 LTPMLLNLLKKNNRIYYDERNETFTFKPLHNIRSGAGLLAYLDSQKTHVGMSIKELRDGW 182

Query: 182 PSVMEDLQALKASGQVWLL-SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLD 241
           P+V  +L+ L+  G+V LL +  D    + + ND      VD + +Q++ EI +P   LD
Sbjct: 183 PNVTVELEELEKQGEVLLLRTRKDGVPKMVWRNDKSCDCHVDKEFQQVWHEIPIP-PTLD 242

Query: 242 VEKDLQKNGMKPAT---NTAKRRANAQHVASSKPKPKKKKQEISKRTKLTNAHL 272
           +  +L K G+KP +   +T KR  + Q      PK KK K   ++R K+TN HL
Sbjct: 243 LASELGKYGLKPTSVDPSTVKRAGHNQ-----TPKQKKPK---TRRGKITNTHL 274

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BLAST of Lsi07G008980 vs. Swiss-Prot
Match: T2EB_BOVIN (General transcription factor IIE subunit 2 OS=Bos taurus GN=GTF2E2 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 4.5e-08
Identity = 52/197 (26.40%), Postives = 99/197 (50.25%), Query Frame = 1


Query: 86  ETRQAFTPEQINEACYVDINSNKAVY---DSLRNNPKVHYDGRRFSYKSKHDLKDKSQLL 145
           +T      E ++E  ++DI   +  +   ++L NNPK+     ++++K K++LKDK  LL
Sbjct: 91  DTHPLTLEEILDETQHLDIGLKQKQWLMSEALVNNPKIEVVDGKYAFKPKYNLKDKKALL 150

Query: 146 YLIRKFPE-GIAVIDLKDAYPSVMEDLQALKASGQVWLLSNFDSQEDIAYPNDPRIQIKV 205
            L+ +  + G+  I L+D    +    +A+KA G   L  N   ++ I + ND   Q  V
Sbjct: 151 RLLDQHDQRGLGGILLEDIEEGLPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSV 210

Query: 206 DDDLKQLFREIEL-PRDMLDVEKDLQKNGMKPATNTAKRRANAQHVASSKPKPKKKKQEI 265
           D++ ++L+R + +   D   +E+ L++ G+    ++  ++  A      KP  +KK    
Sbjct: 211 DEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQDSGPKKV-APIQRRKKPASQKK---- 270

Query: 266 SKRTKLTNAHLPELFKN 278
            +R K  N HL  + K+
Sbjct: 271 -RRFKTHNEHLAGVLKD 281

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BLAST of Lsi07G008980 vs. Swiss-Prot
Match: T2EB_HUMAN (Transcription initiation factor IIE subunit beta OS=Homo sapiens GN=GTF2E2 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.2e-07
Identity = 50/197 (25.38%), Postives = 99/197 (50.25%), Query Frame = 1


Query: 86  ETRQAFTPEQINEACYVDINSNKAVY---DSLRNNPKVHYDGRRFSYKSKHDLKDKSQLL 145
           +T      E ++E  ++DI   +  +   ++L NNPK+     ++++K K++++DK  LL
Sbjct: 93  DTHPLTLDEILDETQHLDIGLKQKQWLMTEALVNNPKIEVIDGKYAFKPKYNVRDKKALL 152

Query: 146 YLIRKFPE-GIAVIDLKDAYPSVMEDLQALKASGQVWLLSNFDSQEDIAYPNDPRIQIKV 205
            L+ +  + G+  I L+D   ++    +A+KA G   L  N   ++ I + ND   Q  V
Sbjct: 153 RLLDQHDQRGLGGILLEDIEEALPNSQKAVKALGDQILFVNRPDKKKILFFNDKSCQFSV 212

Query: 206 DDDLKQLFREIEL-PRDMLDVEKDLQKNGMKPATNTAKRRANAQHVASSKPKPKKKKQEI 265
           D++ ++L+R + +   D   +E+ L++ G+     +  ++  A      KP  +KK    
Sbjct: 213 DEEFQKLWRSVTVDSMDEEKIEEYLKRQGISSMQESGPKKV-APIQRRKKPASQKK---- 272

Query: 266 SKRTKLTNAHLPELFKN 278
            +R K  N HL  + K+
Sbjct: 273 -RRFKTHNEHLAGVLKD 283

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BLAST of Lsi07G008980 vs. Swiss-Prot
Match: T2EB_MOUSE (General transcription factor IIE subunit 2 OS=Mus musculus GN=Gtf2e2 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 2.2e-07
Identity = 51/197 (25.89%), Postives = 101/197 (51.27%), Query Frame = 1


Query: 86  ETRQAFTPEQINEACYVDINSNKAVY---DSLRNNPKVHYDGRRFSYKSKHDLKDKSQLL 145
           +T      E ++E  ++DI   +  +   ++L NNPK+     ++++K K++LKDK  LL
Sbjct: 94  DTHPLTLEEILDETQHLDIGLKQKQWLMTEALVNNPKIEVVDGKYAFKPKYNLKDKKALL 153

Query: 146 YLIRKFPE-GIAVIDLKDAYPSVMEDLQALKASGQVWLLSNFDSQEDIAYPNDPRIQIKV 205
            L+    + G+  I L+D    +    +A+KA G   L  +   ++ I + ND   Q  V
Sbjct: 154 RLLDNHDQRGLGGILLEDIEEGLPNSQKAVKALGDQILFVSRPDKKKILFFNDKSCQFSV 213

Query: 206 DDDLKQLFREIEL-PRDMLDVEKDLQKNGMKPATNTAKRRANAQHVASSKPKPKKKKQEI 265
           D++ ++L+R + +   D   +E+ L++ G+     ++ + +  + VAS + + K   Q+ 
Sbjct: 214 DEEFQKLWRSVTVDSMDEEKIEEYLKRQGI-----SSMQESGPKKVASIQRRKKPASQK- 273

Query: 266 SKRTKLTNAHLPELFKN 278
            +R K  N HL  + K+
Sbjct: 274 KRRFKTHNEHLAGVLKD 284

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BLAST of Lsi07G008980 vs. TrEMBL
Match: A0A0A0KWU5_CUCSA (Transcription initiation factor IIE subunit beta OS=Cucumis sativus GN=Csa_4G049080 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 2.5e-146
Identity = 272/277 (98.19%), Postives = 275/277 (99.28%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTE 60
           MALQEQLDRFKKQQERCQ+TLSGIAASKS SK TPKFTPAPAASIARPPAPAVKFSNDTE
Sbjct: 1   MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60

Query: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120
           RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV
Sbjct: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120

Query: 121 HYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180
           HYDGRRFSYKSKHDLKDK+QLLYL+RKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL
Sbjct: 121 HYDGRRFSYKSKHDLKDKNQLLYLVRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180

Query: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240
           SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR
Sbjct: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240

Query: 241 ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN
Sbjct: 241 ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 277

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BLAST of Lsi07G008980 vs. TrEMBL
Match: D7TW30_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03930 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.1e-121
Identity = 233/280 (83.21%), Postives = 253/280 (90.36%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPT--PKFTPAPAASIARPPAPAVKFSND 60
           MALQE L +FKKQQE+CQ+TL+ IAA   +SKPT   KFTP   A+  +PP PAVKFSND
Sbjct: 1   MALQESLAKFKKQQEKCQSTLTSIAAKAGSSKPTVPQKFTP---AAPTKPPTPAVKFSND 60

Query: 61  TERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNP 120
           TERLQHINSIRK+PVGAQIKRVIDLLLETRQAFTPEQINEACYVD N+NKAV+DSLRNNP
Sbjct: 61  TERLQHINSIRKAPVGAQIKRVIDLLLETRQAFTPEQINEACYVDTNANKAVFDSLRNNP 120

Query: 121 KVHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVW 180
           KV YDG+RF+YKSKHDLKD+SQLL L+RKFPEGIAVIDLKD+YPSVMEDLQALKA+GQVW
Sbjct: 121 KVSYDGKRFAYKSKHDLKDRSQLLVLVRKFPEGIAVIDLKDSYPSVMEDLQALKAAGQVW 180

Query: 181 LLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAK 240
           LLSNFDSQEDIAYPNDPR+ IKVDDDLKQ FR IELPRDMLD+EKDLQKNGMKPATNTAK
Sbjct: 181 LLSNFDSQEDIAYPNDPRVPIKVDDDLKQFFRTIELPRDMLDIEKDLQKNGMKPATNTAK 240

Query: 241 RRANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           RRA AQ H  +SK KPKKKK EISKRTKLTNAHLPELF+N
Sbjct: 241 RRAMAQVHGIASKSKPKKKKHEISKRTKLTNAHLPELFQN 277

[Close]

BLAST of Lsi07G008980 vs. TrEMBL
Match: B9GKK9_POPTR (Transcription initiation factor IIE subunit beta OS=Populus trichocarpa GN=POPTR_0001s09040g PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 5.2e-120
Identity = 235/282 (83.33%), Postives = 253/282 (89.72%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASK----SASKPTPKFTPAPAASIARPPAPAVKFS 60
           MALQEQLDRFKKQQE+CQ+TL+ IA S+    S ++ T    PAP+ S AR PAPAVKFS
Sbjct: 1   MALQEQLDRFKKQQEKCQSTLTSIAKSRPSKSSLTQKTVAVAPAPSTS-ARTPAPAVKFS 60

Query: 61  NDTERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRN 120
           NDTERLQHINSIRK+P GAQIKRVIDLLLETRQAFTPEQIN+ CYVD+NSNKAV+DSLRN
Sbjct: 61  NDTERLQHINSIRKAPAGAQIKRVIDLLLETRQAFTPEQINDHCYVDMNSNKAVFDSLRN 120

Query: 121 NPKVHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQ 180
           NPKVHYDG+RFSYKSKHDLKDKSQLL LIRKFPEGIAVIDLKD+YPSVM+DLQALKA GQ
Sbjct: 121 NPKVHYDGKRFSYKSKHDLKDKSQLLVLIRKFPEGIAVIDLKDSYPSVMDDLQALKAVGQ 180

Query: 181 VWLLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNT 240
           +WLLSNFDSQEDIAYPNDPR+ IKVDDDLKQLFR IELPRDMLD+EKDLQKNGMKPATNT
Sbjct: 181 IWLLSNFDSQEDIAYPNDPRMVIKVDDDLKQLFRGIELPRDMLDIEKDLQKNGMKPATNT 240

Query: 241 AKRRANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           AKRRA AQ    S+K K KKKK EISKRTKLTNAHLPELFKN
Sbjct: 241 AKRRAAAQVQGISTKQKAKKKKHEISKRTKLTNAHLPELFKN 281

[Close]

BLAST of Lsi07G008980 vs. TrEMBL
Match: W9QS39_9ROSA (Transcription initiation factor IIE subunit beta OS=Morus notabilis GN=L484_027256 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 2.6e-119
Identity = 228/278 (82.01%), Postives = 250/278 (89.93%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTE 60
           MALQE+L++FKKQQE CQ+ L+ IAAS  A +PT K TPA   +I++PP PAVKFS DTE
Sbjct: 1   MALQEKLEKFKKQQESCQSILTSIAASNKA-RPTQKVTPAMPPAISKPPPPAVKFSTDTE 60

Query: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120
           RLQHINSIRKSP GAQIKRVIDLLLETRQA TPEQINEACYVD N+NK V+DSLR NPKV
Sbjct: 61  RLQHINSIRKSPAGAQIKRVIDLLLETRQALTPEQINEACYVDTNANKTVFDSLRKNPKV 120

Query: 121 HYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180
           +YDG+RFSYKSKHDLKDKSQLLYL+RKFPEGIAVIDLKDAYP+VMEDLQ+LKA+GQ+WLL
Sbjct: 121 NYDGKRFSYKSKHDLKDKSQLLYLVRKFPEGIAVIDLKDAYPTVMEDLQSLKAAGQIWLL 180

Query: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240
           SN DSQEDIAYPNDPR+ IKVDDDLKQLFR IELPRDMLD+EKDLQKNGMKPATNTA+RR
Sbjct: 181 SNLDSQEDIAYPNDPRVPIKVDDDLKQLFRGIELPRDMLDIEKDLQKNGMKPATNTAQRR 240

Query: 241 ANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           A AQ    SSKPK KKKK EISKRTKLTNAHLPELF+N
Sbjct: 241 AMAQVQGISSKPKTKKKKHEISKRTKLTNAHLPELFQN 277

[Close]

BLAST of Lsi07G008980 vs. TrEMBL
Match: A0A067JRL8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20629 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.7e-118
Identity = 229/278 (82.37%), Postives = 245/278 (88.13%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTE 60
           MALQE+LDRFKKQQE+CQTTL+ IA+  S      K  P  A +  R P P VKFSNDTE
Sbjct: 1   MALQEKLDRFKKQQEKCQTTLTSIASRPSKPAAAQKHVPLAAPTNPRTPGPPVKFSNDTE 60

Query: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120
           RLQHINSIRK+PVGAQIKRVIDLLLETR A+TPEQINEACYVD+ SNKAV+DSLRNNPKV
Sbjct: 61  RLQHINSIRKAPVGAQIKRVIDLLLETRLAYTPEQINEACYVDVTSNKAVFDSLRNNPKV 120

Query: 121 HYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180
            YDG+RFSYKSKHDLKDKSQLL LIRK+PEGIAVIDLKDAYP+VMEDLQALKA+GQ+WLL
Sbjct: 121 TYDGKRFSYKSKHDLKDKSQLLVLIRKYPEGIAVIDLKDAYPTVMEDLQALKAAGQIWLL 180

Query: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240
           SNFDSQEDIAYPNDPR  IKVDDDLKQLFR IELPRDMLD+EKDLQKNGMKPATNTAKRR
Sbjct: 181 SNFDSQEDIAYPNDPRAVIKVDDDLKQLFRGIELPRDMLDIEKDLQKNGMKPATNTAKRR 240

Query: 241 ANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           A AQ    SSKPK KKKK EISKRTKLTNAHLPELF+N
Sbjct: 241 AAAQVQGVSSKPKTKKKKHEISKRTKLTNAHLPELFQN 278

[Close]

BLAST of Lsi07G008980 vs. TAIR10
Match: AT4G20330.1 (AT4G20330.1 Transcription initiation factor TFIIE, beta subunit)

HSP 1 Score: 354.8 bits (909), Expect = 5.0e-98
Identity = 193/280 (68.93%), Postives = 234/280 (83.57%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAA-SIARPPAPAVKFSNDT 60
           MAL+EQLD+F KQQ +CQ+TLS IA+S+  +  + +  P PAA +  +P A  VKFS+DT
Sbjct: 1   MALKEQLDKFNKQQVKCQSTLSSIASSRERTSSSRQNVPLPAAITQKKPDAAPVKFSSDT 60

Query: 61  ERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPK 120
           ERLQ+IN+IRK+PVGAQIKRVIDLL E R A TPEQINE C+VD+++NKAV+DSLR NPK
Sbjct: 61  ERLQNINNIRKAPVGAQIKRVIDLLYERRLALTPEQINEWCHVDMHANKAVFDSLRKNPK 120

Query: 121 VHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWL 180
            HYDGRRFSYK+ HD+ DK+QLL L+RK+ +GIAV+DLKDAYP+VMEDL+AL ASG ++L
Sbjct: 121 AHYDGRRFSYKATHDVNDKNQLLSLVRKYLDGIAVVDLKDAYPNVMEDLKALSASGDIYL 180

Query: 181 LSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKR 240
           LSN  SQEDIAYPND + +IKVDD+ K LFR+I +P DMLDVEK+L K G+KPATNTA+R
Sbjct: 181 LSN--SQEDIAYPNDFKCEIKVDDEFKALFRDINIPNDMLDVEKELLKIGLKPATNTAER 240

Query: 241 RANAQ-HVASSKPK-PKKKKQEISKRTKLTNAHLPELFKN 278
           RA AQ H  S+KPK  KKKKQEISKRTKLTNAHLPELF+N
Sbjct: 241 RAAAQTHGISNKPKDKKKKKQEISKRTKLTNAHLPELFQN 278

[Close]

BLAST of Lsi07G008980 vs. TAIR10
Match: AT4G21010.1 (AT4G21010.1 Transcription initiation factor TFIIE, beta subunit)

HSP 1 Score: 290.8 bits (743), Expect = 8.8e-79
Identity = 167/280 (59.64%), Postives = 217/280 (77.50%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTE 60
           MAL+EQLD+F KQQE+CQ+TLS I++S++A   +  + PA   S  +P     KFS +T+
Sbjct: 1   MALREQLDKFNKQQEKCQSTLSSISSSRTALSRS--YVPAATTS-QKPNVFRGKFSENTK 60

Query: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120
           +LQHI +IR S VGAQ+K VIDLL +TR A+T EQINEACYVD+++NKAV+DSLR NPKV
Sbjct: 61  QLQHITNIRNSAVGAQMKIVIDLLFKTRLAYTAEQINEACYVDMHNNKAVFDSLRKNPKV 120

Query: 121 HYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQV-WL 180
           HYDGRRFSYK+ H++KDK QLL  + K  + I V DLKDAYP+VMEDL++LK+SG++ WL
Sbjct: 121 HYDGRRFSYKATHNIKDKKQLLSFVNKSDKVIDVSDLKDAYPNVMEDLKSLKSSGEIFWL 180

Query: 181 LSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKR 240
           LSN DS+E   Y N+     K+DD+LK LFR+I +P DML+VEK+L K G+KPATN A+R
Sbjct: 181 LSNTDSKEGTVYRNNMEYP-KIDDELKALFRDI-IPSDMLEVEKELLKIGLKPATNIAER 240

Query: 241 RANAQ-HVASSKPK-PKKKKQEISKRTKLTNAHLPELFKN 278
           RA  Q H  S+KPK  KKKK+EI+ RTKLTN+H+ ELF++
Sbjct: 241 RAAEQLHGVSNKPKDKKKKKKEITNRTKLTNSHMLELFQS 275

[Close]

BLAST of Lsi07G008980 vs. NCBI nr
Match: gi|778690905|ref|XP_011653191.1| (PREDICTED: general transcription factor IIE subunit 2-like [Cucumis sativus])

HSP 1 Score: 526.2 bits (1354), Expect = 3.5e-146
Identity = 272/277 (98.19%), Postives = 275/277 (99.28%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTE 60
           MALQEQLDRFKKQQERCQ+TLSGIAASKS SK TPKFTPAPAASIARPPAPAVKFSNDTE
Sbjct: 1   MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPPAPAVKFSNDTE 60

Query: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120
           RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV
Sbjct: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120

Query: 121 HYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180
           HYDGRRFSYKSKHDLKDK+QLLYL+RKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL
Sbjct: 121 HYDGRRFSYKSKHDLKDKNQLLYLVRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180

Query: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240
           SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR
Sbjct: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240

Query: 241 ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN
Sbjct: 241 ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 277

[Close]

BLAST of Lsi07G008980 vs. NCBI nr
Match: gi|659102216|ref|XP_008452012.1| (PREDICTED: general transcription factor IIE subunit 2-like [Cucumis melo])

HSP 1 Score: 522.7 bits (1345), Expect = 3.9e-145
Identity = 272/277 (98.19%), Postives = 274/277 (98.92%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPTPKFTPAPAASIARPPAPAVKFSNDTE 60
           MALQEQLDRFKKQQERCQ+TLSGIAASKS SK TPKFTPAPAASIARP APAVKFSNDTE
Sbjct: 1   MALQEQLDRFKKQQERCQSTLSGIAASKSVSKSTPKFTPAPAASIARPLAPAVKFSNDTE 60

Query: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120
           RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV
Sbjct: 61  RLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNPKV 120

Query: 121 HYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180
           HYDGRRFSYKSKHDLKDK+QLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL
Sbjct: 121 HYDGRRFSYKSKHDLKDKNQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVWLL 180

Query: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240
           SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR
Sbjct: 181 SNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAKRR 240

Query: 241 ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN
Sbjct: 241 ANAQHVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 277

[Close]

BLAST of Lsi07G008980 vs. NCBI nr
Match: gi|225426874|ref|XP_002283557.1| (PREDICTED: general transcription factor IIE subunit 2 [Vitis vinifera])

HSP 1 Score: 443.4 bits (1139), Expect = 3.0e-121
Identity = 233/280 (83.21%), Postives = 253/280 (90.36%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASKSASKPT--PKFTPAPAASIARPPAPAVKFSND 60
           MALQE L +FKKQQE+CQ+TL+ IAA   +SKPT   KFTP   A+  +PP PAVKFSND
Sbjct: 1   MALQESLAKFKKQQEKCQSTLTSIAAKAGSSKPTVPQKFTP---AAPTKPPTPAVKFSND 60

Query: 61  TERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRNNP 120
           TERLQHINSIRK+PVGAQIKRVIDLLLETRQAFTPEQINEACYVD N+NKAV+DSLRNNP
Sbjct: 61  TERLQHINSIRKAPVGAQIKRVIDLLLETRQAFTPEQINEACYVDTNANKAVFDSLRNNP 120

Query: 121 KVHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQVW 180
           KV YDG+RF+YKSKHDLKD+SQLL L+RKFPEGIAVIDLKD+YPSVMEDLQALKA+GQVW
Sbjct: 121 KVSYDGKRFAYKSKHDLKDRSQLLVLVRKFPEGIAVIDLKDSYPSVMEDLQALKAAGQVW 180

Query: 181 LLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNTAK 240
           LLSNFDSQEDIAYPNDPR+ IKVDDDLKQ FR IELPRDMLD+EKDLQKNGMKPATNTAK
Sbjct: 181 LLSNFDSQEDIAYPNDPRVPIKVDDDLKQFFRTIELPRDMLDIEKDLQKNGMKPATNTAK 240

Query: 241 RRANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           RRA AQ H  +SK KPKKKK EISKRTKLTNAHLPELF+N
Sbjct: 241 RRAMAQVHGIASKSKPKKKKHEISKRTKLTNAHLPELFQN 277

[Close]

BLAST of Lsi07G008980 vs. NCBI nr
Match: gi|224058567|ref|XP_002299546.1| (transcription initiation factor-related family protein [Populus trichocarpa])

HSP 1 Score: 438.7 bits (1127), Expect = 7.4e-120
Identity = 235/282 (83.33%), Postives = 253/282 (89.72%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASK----SASKPTPKFTPAPAASIARPPAPAVKFS 60
           MALQEQLDRFKKQQE+CQ+TL+ IA S+    S ++ T    PAP+ S AR PAPAVKFS
Sbjct: 1   MALQEQLDRFKKQQEKCQSTLTSIAKSRPSKSSLTQKTVAVAPAPSTS-ARTPAPAVKFS 60

Query: 61  NDTERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRN 120
           NDTERLQHINSIRK+P GAQIKRVIDLLLETRQAFTPEQIN+ CYVD+NSNKAV+DSLRN
Sbjct: 61  NDTERLQHINSIRKAPAGAQIKRVIDLLLETRQAFTPEQINDHCYVDMNSNKAVFDSLRN 120

Query: 121 NPKVHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQ 180
           NPKVHYDG+RFSYKSKHDLKDKSQLL LIRKFPEGIAVIDLKD+YPSVM+DLQALKA GQ
Sbjct: 121 NPKVHYDGKRFSYKSKHDLKDKSQLLVLIRKFPEGIAVIDLKDSYPSVMDDLQALKAVGQ 180

Query: 181 VWLLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNT 240
           +WLLSNFDSQEDIAYPNDPR+ IKVDDDLKQLFR IELPRDMLD+EKDLQKNGMKPATNT
Sbjct: 181 IWLLSNFDSQEDIAYPNDPRMVIKVDDDLKQLFRGIELPRDMLDIEKDLQKNGMKPATNT 240

Query: 241 AKRRANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           AKRRA AQ    S+K K KKKK EISKRTKLTNAHLPELFKN
Sbjct: 241 AKRRAAAQVQGISTKQKAKKKKHEISKRTKLTNAHLPELFKN 281

[Close]

BLAST of Lsi07G008980 vs. NCBI nr
Match: gi|743852303|ref|XP_011029222.1| (PREDICTED: general transcription factor IIE subunit 2 [Populus euphratica])

HSP 1 Score: 437.6 bits (1124), Expect = 1.7e-119
Identity = 234/282 (82.98%), Postives = 253/282 (89.72%), Query Frame = 1


Query: 1   MALQEQLDRFKKQQERCQTTLSGIAASK----SASKPTPKFTPAPAASIARPPAPAVKFS 60
           MALQEQLDRFKKQQE+CQ+TL+ IA S+    S ++ T    P+P+ S AR PAPAVKFS
Sbjct: 1   MALQEQLDRFKKQQEKCQSTLTSIAKSRPSKSSLTQKTVAVAPSPSTS-ARTPAPAVKFS 60

Query: 61  NDTERLQHINSIRKSPVGAQIKRVIDLLLETRQAFTPEQINEACYVDINSNKAVYDSLRN 120
           NDTERLQHINSIRK+P GAQIKRVIDLLLETRQAFTPEQIN+ CYVD+NSNKAV+DSLRN
Sbjct: 61  NDTERLQHINSIRKAPAGAQIKRVIDLLLETRQAFTPEQINDHCYVDMNSNKAVFDSLRN 120

Query: 121 NPKVHYDGRRFSYKSKHDLKDKSQLLYLIRKFPEGIAVIDLKDAYPSVMEDLQALKASGQ 180
           NPKVHYDG+RFSYKSKHDLKDKSQLL LIRKFPEGIAVIDLKDAYP+VM+DLQALKA GQ
Sbjct: 121 NPKVHYDGKRFSYKSKHDLKDKSQLLVLIRKFPEGIAVIDLKDAYPNVMDDLQALKAVGQ 180

Query: 181 VWLLSNFDSQEDIAYPNDPRIQIKVDDDLKQLFREIELPRDMLDVEKDLQKNGMKPATNT 240
           +WLLSNFDSQEDIAYPNDPR+ IKVDDDLKQLFR IELPRDMLD+EKDLQKNGMKPATNT
Sbjct: 181 IWLLSNFDSQEDIAYPNDPRMVIKVDDDLKQLFRGIELPRDMLDIEKDLQKNGMKPATNT 240

Query: 241 AKRRANAQ-HVASSKPKPKKKKQEISKRTKLTNAHLPELFKN 278
           AKRRA AQ    S+K K KKKK EISKRTKLTNAHLPELFKN
Sbjct: 241 AKRRAAAQVQGISTKQKAKKKKHEISKRTKLTNAHLPELFKN 281

The following BLAST results are available for this feature:

BLAST of Lsi07G008980 vs. Swiss-Prot


Match Name	E-value	Identity	Description
T2EB_DICDI	3.2e-14	26.55	General transcription factor IIE subunit 2 OS=Dictyostelium discoideum GN=gtf2e2...	[more]
T2EB_SCHPO	1.9e-11	27.21	Transcription initiation factor IIE subunit beta OS=Schizosaccharomyces pombe (s...	[more]
T2EB_BOVIN	4.5e-08	26.40	General transcription factor IIE subunit 2 OS=Bos taurus GN=GTF2E2 PE=2 SV=1	[more]
T2EB_HUMAN	2.2e-07	25.38	Transcription initiation factor IIE subunit beta OS=Homo sapiens GN=GTF2E2 PE=1 ...	[more]
T2EB_MOUSE	2.2e-07	25.89	General transcription factor IIE subunit 2 OS=Mus musculus GN=Gtf2e2 PE=1 SV=2	[more]

BLAST of Lsi07G008980 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWU5_CUCSA	2.5e-146	98.19	Transcription initiation factor IIE subunit beta OS=Cucumis sativus GN=Csa_4G049...	[more]
D7TW30_VITVI	2.1e-121	83.21	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0025g03930 PE=4 SV=...	[more]
B9GKK9_POPTR	5.2e-120	83.33	Transcription initiation factor IIE subunit beta OS=Populus trichocarpa GN=POPTR...	[more]
W9QS39_9ROSA	2.6e-119	82.01	Transcription initiation factor IIE subunit beta OS=Morus notabilis GN=L484_0272...	[more]
A0A067JRL8_JATCU	1.7e-118	82.37	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20629 PE=4 SV=1	[more]

BLAST of Lsi07G008980 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G20330.1	5.0e-98	68.93	Transcription initiation factor TFIIE, beta subunit	[more]
AT4G21010.1	8.8e-79	59.64	Transcription initiation factor TFIIE, beta subunit	[more]

BLAST of Lsi07G008980 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778690905\|ref\|XP_011653191.1\|	3.5e-146	98.19	PREDICTED: general transcription factor IIE subunit 2-like [Cucumis sativus]	[more]
gi\|659102216\|ref\|XP_008452012.1\|	3.9e-145	98.19	PREDICTED: general transcription factor IIE subunit 2-like [Cucumis melo]	[more]
gi\|225426874\|ref\|XP_002283557.1\|	3.0e-121	83.21	PREDICTED: general transcription factor IIE subunit 2 [Vitis vinifera]	[more]
gi\|224058567\|ref\|XP_002299546.1\|	7.4e-120	83.33	transcription initiation factor-related family protein [Populus trichocarpa]	[more]
gi\|743852303\|ref\|XP_011029222.1\|	1.7e-119	82.98	PREDICTED: general transcription factor IIE subunit 2 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0005673	transcription factor TFIIE complex

Vocabulary: **Biological Process**
Term	Definition
GO:0006367	transcription initiation from RNA polymerase II promoter

Vocabulary: **INTERPRO**
Term	Definition
IPR016656	TFIIE-bsu
IPR003166	TFIIE_bsu_DNA-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006446	regulation of translational initiation
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0034645	cellular macromolecule biosynthetic process
biological_process	GO:0010467	gene expression
biological_process	GO:0006413	translational initiation
biological_process	GO:0008150	biological_process
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005673	transcription factor TFIIE complex
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G008980.1	Lsi07G008980.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003166	Transcription factor TFIIE beta subunit, DNA-binding domain	PFAM	PF02186	TFIIE_beta	coord: 75..134 score: 1.
IPR003166	Transcription factor TFIIE beta subunit, DNA-binding domain	PROFILE	PS51351	TFIIE_BETA_C	coord: 61..136 score: 10
IPR016656	Transcription initiation factor TFIIE, beta subunit	PIR	PIRSF016398	TFIIE-beta	coord: 1..277 score: 3.3
None	No IPR available	PANTHER	PTHR12716	TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT	coord: 1..277 score: 1.4E
None	No IPR available	PANTHER	PTHR12716:SF8	TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA	coord: 1..277 score: 1.4E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi07G008980	Csa4G049080	Cucumber (Chinese Long) v2	culsiB306
Lsi07G008980	CSPI04G05880	Wild cucumber (PI 183967)	cpilsiB306
Lsi07G008980	Cucsa.106900	Cucumber (Gy14) v1	cgylsiB141
Lsi07G008980	CmaCh16G005860	Cucurbita maxima (Rimu)	cmalsiB328
Lsi07G008980	CmaCh04G007300	Cucurbita maxima (Rimu)	cmalsiB700
Lsi07G008980	CmoCh16G006350	Cucurbita moschata (Rifu)	cmolsiB317
Lsi07G008980	CmoCh04G007750	Cucurbita moschata (Rifu)	cmolsiB694
Lsi07G008980	Cla014643	Watermelon (97103) v1	lsiwmB446
Lsi07G008980	MELO3C016344	Melon (DHL92) v3.5.1	lsimeB457
Lsi07G008980	ClCG07G007970	Watermelon (Charleston Gray)	lsiwcgB415
Lsi07G008980	MELO3C016344.2	Melon (DHL92) v3.6.1	lsimedB475
Lsi07G008980	Cla97C07G135130	Watermelon (97103) v2	lsiwmbB414
Lsi07G008980	Bhi01G001793	Wax gourd	lsiwgoB556
Lsi07G008980	Cp4.1LG14g02310	Cucurbita pepo (Zucchini)	cpelsiB187
Lsi07G008980	CsGy4G005740	Cucumber (Gy14) v2	cgyblsiB280
Lsi07G008980	Carg22211	Silver-seed gourd	carlsiB535
Lsi07G008980	Carg14126	Silver-seed gourd	carlsiB860
Lsi07G008980	CsaV3_4G006050	Cucumber (Chinese Long) v3	cuclsiB325

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi07G008980	Cucurbita pepo (Zucchini)	cpelsiB379