Lsi07G003890 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi07G003890
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ubiquitin thioesterase otubain-like
Location	chr07 : 4169903 .. 4176211 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACAAGGCACGATTGAGATTCAGATTCAGATTCATCGGAATTTTTAGTTTCAGTTTCACCATAGTTGACCTCTTTTCATCTTCTTCTGCAGCCCTAATCCGTCTCGCCACCGTCCACTCCGCTTTCTCCCTCATTTCCATTCCGACGCTTCCTCCATTATCACTTACTGCAACAATGGTTATCTCGCTCCTCCATGGCGCCCTTTTCCCTTTCCTCTTTTAGTATCTGTTTCCCCCAAGCACTGACAAGTGAGCACTGAGCGTCCTTCATTGCCCTCAAGCTCTTCAATCTCCATCTCTCGTCTACCTCTTAATCTGTAAGATTCTTTCTCTTTGTCTCTCCTATTTGCTCCTTGCTTAGTATGGTTTTGTTGGATCATTATCGGATCTGGATTCTGATTGACCGGTACTAGTATGGATGGATTTAAGTTTCGATGCTTGCATCGCTTTCTTGGTTGTAGCCTCTATGCGTGGGAAGTATTTTTGTGTCTACGAGTTTATATGCTTACCGAATATGTTGAGGGAATTTAATAATCTTGGACTTTTTTTTCTTTTCTTTTCTTAAATCAAGTTCGAATTAATGATTTATTACTTTCATTTCCAAGTTTTGGGGAAATTAAAGAAGTAATTTTAAGGTCCTGTTGTTTGCCCTAGTTTCTGATGCGAAGTGCTCAAGCTAATCACTAATGCACAGTGAGAAGTAATGACTCAAGATATGAAGTTTCTATCTCGATGTTATGTTTCATTTTGATGAGCGATTTGGAACCTGATTTTTTTTCCCTTTCCTTTATAACTACGAGCACTTTCCAGTTCTTTAGTAGTATAGCCTACTTTCTTGCAACATGATCTTTAAGAACTCGGCTAAGAAATTGGATGTCGACTGTATTAGGTTTTTAAACATTGGATTTATAGATGTGCTTGTTAAAAGTGTGGAATAAGTAATTGCGCTGGTCTGATATTGATATGCAATGTTTTCTTAATAACTGCTGTGGTCTATATTCACTGGCATTCAATTATCCGTATCATATGGTTTGAGAATGATCGAGTTATTAACTTTAAAGATGAGAATAAATATGCTATTGCTACACTTTTTTTTTTTTTTTTTTTTTTTACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTGCTACACATTTATTTATTACTAGATAATGTTTCTTGAACGCTCAGCAGGTGTTCTCCTAGCAGGCAAGTTTATGAGCTGGTTGGATCATCACAGTGAAGTTACAAGGGAAATTCTTGGATAGGTTTTTCAAACCCGACTTCACTTACTGAGGAAACAAAGTATTTGTATTCCTTTAAAAGAATGCAGAACCAGGAAAATGTTGATGCAGATGGGAAAGCGGAGTCTGTAATATCTATTCAAACATCAGAGTACGATAGTTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTTGGTGACAAGGCATGTTTCACCTAACTAAGTTTTAATCTCCTGCATTTTCCTGTCTTGATCTTGCCACACTTTTCCCTCCCTTTCTTCCTTTTGTTTGTGTTATCTATGATTCTTTGCATGTAGGCAACCAAGTTTTACTTTTTAGTCTTCCCTTCTTTTCTCATAAATTATGCTATGAAGTAGTGCTCTTATTTTATTTGTTATAACATGTTTGGGTAACTCCCAAAATGTCGTCTAGCAACACAGTTGCTAATGTCTAACATATGTTTGGAAATTATTTGGAACTTAGAAATGTTGGAGGGATTCTGCATCGCTTTTTGCTTCAAAAGCCACCCTTTCTGTATGTTTAATGGCATTTTTGGTGTCTAACCTTTGGGCCTACGTATCTAACCCTTTTTGTAATGTTACTATTTGTTTTTAATTCTTATTTTAACAATTGGTGGTCTATCTTGTTAGTTCTTTTTTCCCCCTACTTTATTTTGGCTTGGCTTGGCTCTATTTTTGCATTTTTATTTATCAAAAGTGAACCTTTATATCTCTGTTTTTTTTCCATTTTTACTCATTTTGGAGTTTGTATCCTTCGAGCATTTGTTTCTCATTTTGTTTTTCATTTTTTTAATTGTATGTTTTGAATGCCTTACAACTGTGGTTTTATAGCTCTAAGATGTTTTTATCAGTTGATTTTTCTTTTACTCCATAATTAAACTTAATGGCAATGGTGTTGCAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCCGGAAGTCCCATTTTGCTGGAGAAAATTAAGGTTCTATTTGTTGCTTATTGTTATTTTAGTAGAGTTATTGTCCTTGGAATAATTATTTATGTTAAATGTTACATGTTATGCAGGTGCTCAGTGGCCAATATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGCAGCTTTATGTTTTCGTATCTTGTAAGTCTAATTCTTTTAATTAATCTCAGCAGCTTGATTATTGTACTTTTTGTGCATCATCGAGCATTGGCTGTTACTGACTTGGCCTCTCAAATCAGGAACATATTTTAGAGTCTCAAGACAGAACTGAAGTTGATCGTATCAAAACAAATGTGGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACTTTCGAAGATTTTTTTGCGGTAAATTGTTACTATTCCCTTTTTAAAATTAATTTATTATACATATATCACCCTCTGCTTTAAGCAGTATGGCTTATTTCTCCCGTTTAACGGGAGGCAGTGGAAGTTGTGAACTTCTTCCTAGTCTCTAAATGTGTTATTGAACATAGCAGATAGAAATATCGCTACAACAAGTTTATTGTTGCCGAGATGTTTTCTTTGCAATGTGTGATGCTAGAGTAGGTTTATTCTCGAGCTTGTTTATGGCTTAAATTTGAGCGACTTGTCAAGGAGTTACACTGATGCAGATATGCATAATTTTGCCAATAGGGAGTCTTTAACCTTGACCTTGTGGGGATACCACATCTTACCTTCTTTTGAATTGGGTTTTATGTTAATATAATTCATTAACTCTCAGATTCGCATAAATAATTGTGTTTGCTCTTTCCTCGTGTTTATCAAGGGGGATTGTGCTCCTGGATTATTGGTTTAAGGAGCCTCAGAAAGCTTGTGGTCAAGTGTTTTACTTGCTTAGCTAGGTTTTACACCTTGCCTCATTGACTTTGTTTCAAAAAGACCCCAATTGGTTTATGATTTTTATCATTTAAAAATATACTATGGTCAAATGAGTAAATAAAGAAAAAATCAAACAAAACGTTTGCTGTTTTACTTTATTGAACCTTTTCACCTAAGGATGTGCTCTTTCTCTCTGATTGATGGATAATTGGATAGGAATGAGGAAATTAGACCAAACTAAAGAGGAAACTATGGAGGGTTGCAAGTTTAATGATAGAAAAAGTGGCGATGATAATCTATTATTGCGATGACAGATTTTAACTTTAACATCTTTACAAATTTGCAAGGACATTTGTAGTTTTTGTAATTGAATTTATTTGTTTTTGTTCTCTATCTCATAGTTCATGCTAACTTTTAATATATGAACATTTTATATATTGTGTCTTCACCTCATTGAAGTCCTGATTCTTTTTTGTTACTCAGTCACATATAAACTCTAAAGAGCTATTCTGCTTCAGTGTGTTTGCCACTTCTATAAACATTGTCCCAGATTTATATCACCTTAAAGATGTCTAGAGTCTAGAGTCTAGACTCATCCTTCTTTATTATTGCCTTCTTTATTTCATTTCGGTATAAACTAGCTAGATATCCTTGGTGGTTAATGATGGATGCCTTAAATATAAATTTTTTATTAATAAATATGTTTAAAATATCTTTACAGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAAACTTCTATAAGGTGTCAATAACCTGCCCTTCTACCTTGGAATTTTGATTCCCGAATTTTTATTTTATAGTGGAATCTTCTGTTGCCATCGATTATGAAATTCTGCCATTCATGATTGAATTTGACTCTTATTTTTCTTGGATGCAGTCATGATGAACTTGTAACTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTGATGTTTGTACATTCAATATCCTATTGCCTCTTTCTGTTTCTACTGACAAAATTGTTTTGCTAGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCGGAATTTTTTGAGCCTTTTATTCTGGGATTGACAAATGGCACAGTTGATCAGGTTTGTTAAATTTATAATAGCGTCTACAAGTTTATCATTATGTGTTGACTTTTATCTTCAGATTTATGTTGGAAATTTAACCATACAAGTCATTTGAAATCTAAGTTGATTTGGTTAGGGTGACCCTTTTGTAAGTGTAGTCTCGAGAAGAAGCAGTTAATAGTTAATGTGCAATAATGATGATGTTAGACCATGTATCCTTGGACAGGAATAAGAATGGATCTTCCCGATTTCCATTCTTGTTGTTTACTTCATTTGGGGAATAGAATAAGTACTACCCACTTAAAAATGGAAAACCGTGCTCTTAGTTTTTAGCAACATCTTTACTTCACAAAAGATAATCATTCTCCTATTGCTGTTCTTTGGGTTCAATTTCTTTTCCTAAATCTCTACTTTTTTGTGGGTCTTGCATTTGTTGAACTTTGATTCTCATTCTAAGAATCTGAATGTTAACCAACAATATCAACCACAATTTTATTCTTACTGATCCAGATTTCATAGTAATTTTAGTAAACAAAGTATTTAAAATTTTATTTCAAATCCATAATTTTTCTATCCAATCAGTCGTGATGATTCTTGTACGGTTTTATTCCCATTAAATAAACCATGCCTTAGGTTAATTATTTTATTATCGCATCTATGTTCAAATGAAATCACTTCAGCCTTGTTGTTTTCCCTTTTTTGAGGTCACGTGGGGGATTCGAATTTCTAACCTTTTGGTCGGCTATTATTTCCTTTTTTGTGCATCAGAATCCCAAACAAACAAACAGTAAGCCTTTGGTCTTTATGTTTGGGCGTTAAATTTTCATTTTTAGTTTTCTCTTTTTTGTGGGGAAATTTTGTTGGGCGTATATTAGCTTTCTAAGATGTCAATGGACATTTAATTTCTTATTTAAGAAGAAAGAGAACCTGTTTATCATGAATTCCAATCTCATTCTCTTTGGCACAGTTCTGCAAATCAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGGCGATTCGTGTTCTGTACCTCGATCGGAGCTCTTGTGATTCTGGTGGCCTCAGTGTAAATCACCATGATTTTGTTCCTGCTGCTAGTGAGGTCTCAAGTGATAGTGGTGCTGGCTCTGAGATTAAGATCCCTTTCATCACTTTGCTCTATCGTCCCGGTCATTATGATATCCTTTATCCAAAGTGATGGTAAACAGCTCTCATGAACTTATTTGAAAAATTGCCACAGAAATGTCTCTTCCTCCCACCTTTTATATGCAACAGATTAACTTTATAGTCTGTATAAAATGATTATGTTTAAAAGTATATTGTCGCTGCCATCCTCATTCCTCATGCAAAATGATATTTTCATCTGTAGTTGTCTACCATTATTTGCAAATTTGTTTCATGTCTTTGATTTACTTGGGAATTTTATTCAGTGTTGGAGGTTTTTTGTTCTGGATGGTTCAGCTTGAATTTTATGTCATGTATTTCAGGCCATTGGCAAACCCTGGTCGGGCTCTCAGAGTTGAACTGCATTGACATGACAGACATGGATCCAAGCTTCAACAAGATAAAGGTTGGATTTCATAATTGATGCTTGCCAGATGTTCTGTACTGATTACTGATGAGCTGTTTGAGTAT

mRNA sequence

AAACAAGGCACGATTGAGATTCAGATTCAGATTCATCGGAATTTTTAGTTTCAGTTTCACCATAGTTGACCTCTTTTCATCTTCTTCTGCAGCCCTAATCCGTCTCGCCACCGTCCACTCCGCTTTCTCCCTCATTTCCATTCCGACGCTTCCTCCATTATCACTTACTGCAACAATGGTTATCTCGCTCCTCCATGGCGCCCTTTTCCCTTTCCTCTTTTAGTATCTGTTTCCCCCAAGCACTGACAAGTGAGCACTGAGCGTCCTTCATTGCCCTCAAGCTCTTCAATCTCCATCTCTCGTCTACCTCTTAATCTCAGGTGTTCTCCTAGCAGGCAAGTTTATGAGCTGGTTGGATCATCACAGTGAAGTTACAAGGGAAATTCTTGGATAGGTTTTTCAAACCCGACTTCACTTACTGAGGAAACAAAGTATTTGTATTCCTTTAAAAGAATGCAGAACCAGGAAAATGTTGATGCAGATGGGAAAGCGGAGTCTGTAATATCTATTCAAACATCAGAGTACGATAGTTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTTGGTGACAAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCCGGAAGTCCCATTTTGCTGGAGAAAATTAAGGTGCTCAGTGGCCAATATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGCAGCTTTATGTTTTCGTATCTTGAACATATTTTAGAGTCTCAAGACAGAACTGAAGTTGATCGTATCAAAACAAATGTGGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACTTTCGAAGATTTTTTTGCGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAAACTTCTATAAGTCATGATGAACTTGTAACTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTTTGTTACATCAGGTGAAATACAAAAGCGTTCGGAATTTTTTGAGCCTTTTATTCTGGGATTGACAAATGGCACAGTTGATCAGTTCTGCAAATCAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGGCGATTCGTGTTCTGTACCTCGATCGGAGCTCTTGTGATTCTGGTGGCCTCAGTGTAAATCACCATGATTTTGTTCCTGCTGCTAGTGAGGTCTCAAGTGATAGTGGTGCTGGCTCTGAGATTAAGATCCCTTTCATCACTTTGCTCTATCGTCCCGGTCATTATGATATCCTTTATCCAAAGTGATGGCCATTGGCAAACCCTGGTCGGGCTCTCAGAGTTGAACTGCATTGACATGACAGACATGGATCCAAGCTTCAACAAGATAAAGGTTGGATTTCATAATTGATGCTTGCCAGATGTTCTGTACTGATTACTGATGAGCTGTTTGAGTAT

Coding sequence (CDS)

ATGCAGAACCAGGAAAATGTTGATGCAGATGGGAAAGCGGAGTCTGTAATATCTATTCAAACATCAGAGTACGATAGTTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTTGGTGACAAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCCGGAAGTCCCATTTTGCTGGAGAAAATTAAGGTGCTCAGTGGCCAATATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGCAGCTTTATGTTTTCGTATCTTGAACATATTTTAGAGTCTCAAGACAGAACTGAAGTTGATCGTATCAAAACAAATGTGGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACTTTCGAAGATTTTTTTGCGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAAACTTCTATAAGTCATGATGAACTTGTAACTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTTTGTTACATCAGGTGAAATACAAAAGCGTTCGGAATTTTTTGAGCCTTTTATTCTGGGATTGACAAATGGCACAGTTGATCAGTTCTGCAAATCAGCAGTTGAACCAATGGGTGAAGAGAGTGATCATGTACACATAATTGCCTTATCTGATGCTTTGGGTGTGGCGATTCGTGTTCTGTACCTCGATCGGAGCTCTTGTGATTCTGGTGGCCTCAGTGTAAATCACCATGATTTTGTTCCTGCTGCTAGTGAGGTCTCAAGTGATAGTGGTGCTGGCTCTGAGATTAAGATCCCTTTCATCACTTTGCTCTATCGTCCCGGTCATTATGATATCCTTTATCCAAAGTGA

Protein sequence

MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISDYGQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDILYPK

Homology

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BLAST of Lsi07G003890 vs. Swiss-Prot
Match: OTUBL_ARATH (Ubiquitin thioesterase otubain-like OS=Arabidopsis thaliana GN=At1g28120 PE=2 SV=2)

HSP 1 Score: 412.9 bits (1060), Expect = 2.9e-114
Identity = 211/303 (69.64%), Postives = 241/303 (79.54%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ ++  D +AE   SI   + + WGN    +    + +   +EA KVP+VGDKEPLS
Sbjct: 1   MQNQIDMVKD-EAEVAASISAIKGEEWGNCSSVE---DQPSFQEEEAAKVPYVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGSPILLEKIK+L  QY  IRRTRGDGNCFFRSFMFSYLEHILESQDR EVDR
Sbjct: 61  SLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL++LGYT+FTFEDFFALFLEQL+  LQG E SIS+DELV RSRDQS+SD
Sbjct: 121 IKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVT+G+I+ R++FFEPFI GL+N TVDQFCKS+VEPMGEESDH+HI ALSDAL
Sbjct: 181 YIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GVAIRV+YLDRSSCDSGG++VNHHDFVP       D     E   PFITLLYRPGHYDIL
Sbjct: 241 GVAIRVVYLDRSSCDSGGVTVNHHDFVPVGITNEKD----EEASAPFITLLYRPGHYDIL 295

[Close]

BLAST of Lsi07G003890 vs. Swiss-Prot
Match: OTUB1_HUMAN (Ubiquitin thioesterase OTUB1 OS=Homo sapiens GN=OTUB1 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 116/270 (42.96%), Postives = 155/270 (57.41%), Query Frame = 1


Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ IM Q+  I  + A + P V ++  LS L  EY     I  +KIK L  +Y+ IR+TR
Sbjct: 27  DEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTR 86

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCF+R+F FS+LE +L+  D  E+ R K      ++ L S G+TEFT EDF   F++
Sbjct: 87  PDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMD 146

Query: 152 QLESALQGNETSISHDELVTRSRDQSISD----YGQFVTSGEIQKRSEFFEPFILGLTNG 211
            +E      E   S  +L+    DQS SD    Y + +TSG +Q+ S+FFE FI G    
Sbjct: 147 LIEQV----EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GR 206

Query: 212 TVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAAS 271
           TV +FC+  VEPM +ESDH+HIIAL+ AL V+I+V Y+DR      G + N H F P  S
Sbjct: 207 TVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHIF-PEGS 266

Query: 272 EVSSDSGAGSEIKIPFITLLYRPGHYDILY 298
           E             P + LLYRPGHYDILY
Sbjct: 267 E-------------PKVYLLYRPGHYDILY 270

[Close]

BLAST of Lsi07G003890 vs. Swiss-Prot
Match: OTUB1_MOUSE (Ubiquitin thioesterase OTUB1 OS=Mus musculus GN=Otub1 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 116/270 (42.96%), Postives = 155/270 (57.41%), Query Frame = 1


Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ IM Q+  I  + A + P V ++  LS L  EY     I  +KIK L  +Y+ IR+TR
Sbjct: 27  DEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTR 86

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCF+R+F FS+LE +L+  D  E+ R K      ++ L S G+TEFT EDF   F++
Sbjct: 87  PDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMD 146

Query: 152 QLESALQGNETSISHDELVTRSRDQSISD----YGQFVTSGEIQKRSEFFEPFILGLTNG 211
            +E      E   S  +L+    DQS SD    Y + +TSG +Q+ S+FFE FI G    
Sbjct: 147 LIEQV----EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GR 206

Query: 212 TVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAAS 271
           TV +FC+  VEPM +ESDH+HIIAL+ AL V+I+V Y+DR      G + N H F P  S
Sbjct: 207 TVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHVF-PEGS 266

Query: 272 EVSSDSGAGSEIKIPFITLLYRPGHYDILY 298
           E             P + LLYRPGHYDILY
Sbjct: 267 E-------------PKVYLLYRPGHYDILY 270

[Close]

BLAST of Lsi07G003890 vs. Swiss-Prot
Match: OTUB1_RAT (Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus GN=Otub1 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 116/270 (42.96%), Postives = 155/270 (57.41%), Query Frame = 1


Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ IM Q+  I  + A + P V ++  LS L  EY     I  +KIK L  +Y+ IR+TR
Sbjct: 27  DEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTR 86

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCF+R+F FS+LE +L+  D  E+ R K      ++ L S G+TEFT EDF   F++
Sbjct: 87  PDGNCFYRAFGFSHLEALLD--DSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMD 146

Query: 152 QLESALQGNETSISHDELVTRSRDQSISD----YGQFVTSGEIQKRSEFFEPFILGLTNG 211
            +E      E   S  +L+    DQS SD    Y + +TSG +Q+ S+FFE FI G    
Sbjct: 147 LIEQV----EKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEG--GR 206

Query: 212 TVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPAAS 271
           TV +FC+  VEPM +ESDH+HIIAL+ AL V+I+V Y+DR      G + N H F P  S
Sbjct: 207 TVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGE----GGTTNPHVF-PEGS 266

Query: 272 EVSSDSGAGSEIKIPFITLLYRPGHYDILY 298
           E             P + LLYRPGHYDILY
Sbjct: 267 E-------------PKVYLLYRPGHYDILY 270

[Close]

BLAST of Lsi07G003890 vs. Swiss-Prot
Match: OTUBL_DROME (Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster GN=CG4968 PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 4.6e-43
Identity = 113/273 (41.39%), Postives = 154/273 (56.41%), Query Frame = 1


Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ I+ QK  I  + ++  P V ++ PL+ L AEY SG  I   KI+ LS +Y  IRRTR
Sbjct: 12  DELIIQQKRDIEKEISDTTPLVSEQLPLTCLYAEY-SGDEIFTAKIQDLSKKYKFIRRTR 71

Query: 92  GDGNCFFRSFMFSYLEHILESQDRTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCFFR+F +SYLE+++   + +     K   E  ++ L  LG+  FT EDF   F+E
Sbjct: 72  PDGNCFFRAFAYSYLEYLI--SNTSAYQEFKKLAEESKEKLVQLGFPSFTLEDFHETFME 131

Query: 152 QLESALQGNE--TSISHDELVTRSRDQSISDYG----QFVTSGEIQKRSEFFEPFILGLT 211
            ++     N    S   DEL     +Q  SDY     + +TSG++Q+ ++F++ FI G  
Sbjct: 132 VIQRVSPDNAGGHSTVQDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFYQNFIEG-- 191

Query: 212 NGTVDQFCKSAVEPMGEESDHVHIIALSDALGVAIRVLYLDRSSCDSGGLSVNHHDFVPA 271
           + T++ F    VEPM +ESDH+HIIAL  ALG  +RV YLDR      G +V  HDF P 
Sbjct: 192 DLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYLDRGE----GGTVKAHDF-PE 251

Query: 272 ASEVSSDSGAGSEIKIPFITLLYRPGHYDILYP 299
            SE             P I L+YRPGHYDILYP
Sbjct: 252 GSE-------------PRIYLIYRPGHYDILYP 261

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BLAST of Lsi07G003890 vs. TrEMBL
Match: A0A0A0KX37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007720 PE=4 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.1e-155
Identity = 283/303 (93.40%), Postives = 292/303 (96.37%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+DADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISH+ELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EV+S S A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGS-AASEIKIPFITLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. TrEMBL
Match: M5XZ09_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007269mg PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 2.7e-130
Identity = 233/303 (76.90%), Postives = 261/303 (86.14%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ V  DG+ ES  S+ T E+D W N GD DIM Q SAI  +EAEK+PF+GDKEPLS
Sbjct: 74  MQNQDGVQVDGEVESATSVPTPEFDEWANFGDQDIMQQHSAIRAEEAEKIPFLGDKEPLS 133

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 134 SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 193

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE SISHDEL+ RSRDQS+SD
Sbjct: 194 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEASISHDELILRSRDQSVSD 253

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI+KR+EFFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 254 YVVMFFRFVTSGEIRKRAEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 313

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD+GG+SVNHHDFVPA S++ + SG   ++  PFITLLYRPGHYDIL
Sbjct: 314 GVPIRVVYLDRSSCDTGGVSVNHHDFVPAGSDLPNASGCSEKVS-PFITLLYRPGHYDIL 373

[Close]

BLAST of Lsi07G003890 vs. TrEMBL
Match: A0A061E5K2_THECC (Otubain, Ubiquitin thioesterase Otubain, Peptidase C65, otubain isoform 1 OS=Theobroma cacao GN=TCM_008341 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 1.8e-126
Identity = 229/303 (75.58%), Postives = 256/303 (84.49%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQN+E V ADG+ ES +SI  SE+D W    DDDIM Q+SA   +EA+KVPFVGDKEPL+
Sbjct: 1   MQNREGVVADGEKESAVSIPVSEFDDWAKFADDDIMQQQSAFHAEEAKKVPFVGDKEPLT 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
            L AEY+SGSPILLEKIK+L  QYAAIRRTRGDGNCFFRSFMFSYLE++LESQD  EVDR
Sbjct: 61  MLAAEYESGSPILLEKIKLLGQQYAAIRRTRGDGNCFFRSFMFSYLEYVLESQDHAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE SIS DEL+ RSRDQSISD
Sbjct: 121 IKGNVEECRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEDSISQDELIIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI+KRSEFFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD+GG+SVNHHDFVP +   S+ +   +E   PFITLLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDNGGVSVNHHDFVPTSGGHSNATSGSTEPVSPFITLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. TrEMBL
Match: A0A0D2PW37_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G197000 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 8.9e-126
Identity = 230/303 (75.91%), Postives = 254/303 (83.83%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+   AD + ES +SI  SE D W N  DDDIM Q+SAI  +EA+K+PFVGDKE LS
Sbjct: 1   MQNQDGAVADREKESAVSIPISEVDDWANFADDDIMQQQSAIHAEEAKKIPFVGDKEALS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
            L AEY+SGSPILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQDR EVDR
Sbjct: 61  MLAAEYESGSPILLEKIKVLDQQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL+SLG+T+FTFEDFF+LFLEQLE  LQGNE SIS DEL+ RSRDQSISD
Sbjct: 121 IKGNVEECRKTLQSLGHTDFTFEDFFSLFLEQLECVLQGNEDSISQDELILRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI+KRSEFFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD GG+SVNHHDF+P + + S+  G  +    PFITLLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDIGGVSVNHHDFLPTSGDKSNAKGGSTVPVKPFITLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. TrEMBL
Match: A0A067JKE5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23096 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-124
Identity = 223/303 (73.60%), Postives = 256/303 (84.49%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQE   ADG+AE+  +   SE++ W N  D+DIM   S I   EA+K+PF+GDKEPLS
Sbjct: 1   MQNQEGQLADGEAEAKATPAPSEFEDWANFADEDIMQHHSTIRSVEADKIPFLGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           +L AEY+SGSPILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHIL++QDR EVDR
Sbjct: 61  ALAAEYQSGSPILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILQTQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           +K  VE CRKTL+SLGY +FTFEDFFALFLEQL+  LQGNETS++HDEL+ RSRDQSISD
Sbjct: 121 VKIKVEECRKTLQSLGYADFTFEDFFALFLEQLDCVLQGNETSVNHDELINRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI++RS+FFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRRRSDFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD+GG+SVNHHDF+P AS+V + +  GS  K PFI LLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDTGGVSVNHHDFIPMASDVPNPATDGSVTKNPFINLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. TAIR10
Match: AT1G28120.1 (AT1G28120.1 Ovarian tumour, otubain (InterPro:IPR003323), Ubiquitin thioesterase Otubain (InterPro:IPR016615), Peptidase C65, otubain (InterPro:IPR019400))

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-115
Identity = 211/303 (69.64%), Postives = 241/303 (79.54%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ ++  D +AE   SI   + + WGN    +    + +   +EA KVP+VGDKEPLS
Sbjct: 1   MQNQIDMVKD-EAEVAASISAIKGEEWGNCSSVE---DQPSFQEEEAAKVPYVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGSPILLEKIK+L  QY  IRRTRGDGNCFFRSFMFSYLEHILESQDR EVDR
Sbjct: 61  SLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL++LGYT+FTFEDFFALFLEQL+  LQG E SIS+DELV RSRDQS+SD
Sbjct: 121 IKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVT+G+I+ R++FFEPFI GL+N TVDQFCKS+VEPMGEESDH+HI ALSDAL
Sbjct: 181 YIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GVAIRV+YLDRSSCDSGG++VNHHDFVP       D     E   PFITLLYRPGHYDIL
Sbjct: 241 GVAIRVVYLDRSSCDSGGVTVNHHDFVPVGITNEKD----EEASAPFITLLYRPGHYDIL 295

[Close]

BLAST of Lsi07G003890 vs. NCBI nr
Match: gi|659108333|ref|XP_008454143.1| (PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo])

HSP 1 Score: 560.1 bits (1442), Expect = 2.4e-156
Identity = 284/303 (93.73%), Postives = 292/303 (96.37%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+N+D DGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQDNLDTDGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISHDELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EVSSDS A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDS-AASEIKIPFITLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. NCBI nr
Match: gi|449468900|ref|XP_004152159.1| (PREDICTED: ubiquitin thioesterase otubain-like [Cucumis sativus])

HSP 1 Score: 557.4 bits (1435), Expect = 1.5e-155
Identity = 283/303 (93.40%), Postives = 292/303 (96.37%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQEN+DADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+TEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNE+SISH+ELV RSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEIQKRSEFFEPFI+GLTNGTVDQFCK+AVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRVLYLDRSSCDSGGLSVNHHDFVPA +EV+S S A SEIKIPFITLLYRPGHYDIL
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGS-AASEIKIPFITLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. NCBI nr
Match: gi|657956293|ref|XP_008369628.1| (PREDICTED: ubiquitin thioesterase otubain-like [Malus domestica])

HSP 1 Score: 474.9 bits (1221), Expect = 1.0e-130
Identity = 237/303 (78.22%), Postives = 260/303 (85.81%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ V  DG+ ES  S+ T E+D W N GD DIMLQ SAI  +EAEK+PFVGDKEPLS
Sbjct: 1   MQNQDGVQVDGEVESAASVATPEFDEWANFGDQDIMLQHSAIRAEEAEKIPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 61  SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE SISHDEL+ RSRDQS+SD
Sbjct: 121 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEESISHDELILRSRDQSVSD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI+KRSEFFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD+GG+SVNHHDF+PA S+    S  GSE   PFITLLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDNGGVSVNHHDFIPAGSDAPKAS-EGSEKVSPFITLLYRPGHYDIL 300

[Close]

BLAST of Lsi07G003890 vs. NCBI nr
Match: gi|596182099|ref|XP_007223296.1| (hypothetical protein PRUPE_ppa007269mg [Prunus persica])

HSP 1 Score: 473.0 bits (1216), Expect = 3.8e-130
Identity = 233/303 (76.90%), Postives = 261/303 (86.14%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ V  DG+ ES  S+ T E+D W N GD DIM Q SAI  +EAEK+PF+GDKEPLS
Sbjct: 74  MQNQDGVQVDGEVESATSVPTPEFDEWANFGDQDIMQQHSAIRAEEAEKIPFLGDKEPLS 133

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 134 SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 193

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE SISHDEL+ RSRDQS+SD
Sbjct: 194 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEASISHDELILRSRDQSVSD 253

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI+KR+EFFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 254 YVVMFFRFVTSGEIRKRAEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 313

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD+GG+SVNHHDFVPA S++ + SG   ++  PFITLLYRPGHYDIL
Sbjct: 314 GVPIRVVYLDRSSCDTGGVSVNHHDFVPAGSDLPNASGCSEKVS-PFITLLYRPGHYDIL 373

[Close]

BLAST of Lsi07G003890 vs. NCBI nr
Match: gi|645231113|ref|XP_008222244.1| (PREDICTED: ubiquitin thioesterase otubain-like [Prunus mume])

HSP 1 Score: 472.6 bits (1215), Expect = 5.0e-130
Identity = 235/303 (77.56%), Postives = 261/303 (86.14%), Query Frame = 1


Query: 1   MQNQENVDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ V  DG+ ES  S+ T E+D W N GD DIM Q SAI  +EAEK+PF+GDKEPLS
Sbjct: 1   MQNQDGVQVDGEVESATSVPTPEFDEWANFGDQDIMQQHSAIRAEEAEKIPFLGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 61  SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNETSISHDELVTRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S L GNE SISHDEL+ RSRDQS+SD
Sbjct: 121 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLHGNEASISHDELILRSRDQSVSD 180

Query: 181 Y----GQFVTSGEIQKRSEFFEPFILGLTNGTVDQFCKSAVEPMGEESDHVHIIALSDAL 240
           Y     +FVTSGEI+KR+EFFEPFILGLTN TV+QFCKS+VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRAEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVAIRVLYLDRSSCDSGGLSVNHHDFVPAASEVSSDSGAGSEIKIPFITLLYRPGHYDIL 300
           GV IRV+YLDRSSCD+GG+SVNHHDFVPA S++ + SG GSE   PFITLLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDTGGVSVNHHDFVPAGSDLPNASG-GSEKVSPFITLLYRPGHYDIL 300

The following BLAST results are available for this feature:

BLAST of Lsi07G003890 vs. Swiss-Prot


Match Name	E-value	Identity	Description
OTUBL_ARATH	2.9e-114	69.64	Ubiquitin thioesterase otubain-like OS=Arabidopsis thaliana GN=At1g28120 PE=2 SV...	[more]
OTUB1_HUMAN	1.9e-44	42.96	Ubiquitin thioesterase OTUB1 OS=Homo sapiens GN=OTUB1 PE=1 SV=2	[more]
OTUB1_MOUSE	1.9e-44	42.96	Ubiquitin thioesterase OTUB1 OS=Mus musculus GN=Otub1 PE=1 SV=2	[more]
OTUB1_RAT	1.9e-44	42.96	Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus GN=Otub1 PE=1 SV=1	[more]
OTUBL_DROME	4.6e-43	41.39	Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster GN=CG4968 PE=2 SV...	[more]

BLAST of Lsi07G003890 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX37_CUCSA	1.1e-155	93.40	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007720 PE=4 SV=1	[more]
M5XZ09_PRUPE	2.7e-130	76.90	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007269mg PE=4 SV=1	[more]
A0A061E5K2_THECC	1.8e-126	75.58	Otubain, Ubiquitin thioesterase Otubain, Peptidase C65, otubain isoform 1 OS=The...	[more]
A0A0D2PW37_GOSRA	8.9e-126	75.91	Uncharacterized protein OS=Gossypium raimondii GN=B456_005G197000 PE=4 SV=1	[more]
A0A067JKE5_JATCU	1.7e-124	73.60	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23096 PE=4 SV=1	[more]

BLAST of Lsi07G003890 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G28120.1	1.7e-115	69.64	Ovarian tumour, otubain (InterPro:IPR003323), Ubiquitin thioesterase...	[more]

BLAST of Lsi07G003890 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659108333\|ref\|XP_008454143.1\|	2.4e-156	93.73	PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo]	[more]
gi\|449468900\|ref\|XP_004152159.1\|	1.5e-155	93.40	PREDICTED: ubiquitin thioesterase otubain-like [Cucumis sativus]	[more]
gi\|657956293\|ref\|XP_008369628.1\|	1.0e-130	78.22	PREDICTED: ubiquitin thioesterase otubain-like [Malus domestica]	[more]
gi\|596182099\|ref\|XP_007223296.1\|	3.8e-130	76.90	hypothetical protein PRUPE_ppa007269mg [Prunus persica]	[more]
gi\|645231113\|ref\|XP_008222244.1\|	5.0e-130	77.56	PREDICTED: ubiquitin thioesterase otubain-like [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0016579	protein deubiquitination

Vocabulary: **INTERPRO**
Term	Definition
IPR019400	Peptidase_C65_otubain
IPR016615	Otubain
IPR003323	OTU_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0006520	cellular amino acid metabolic process
biological_process	GO:0071108	protein K48-linked deubiquitination
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0004843	thiol-dependent ubiquitin-specific protease activity
molecular_function	GO:0043130	ubiquitin binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi07G003890.1	Lsi07G003890.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003323	OTU domain	PROFILE	PS50802	OTU	coord: 85..299 score: 12
IPR016615	Ubiquitin thioesterase Otubain	PIR	PIRSF013503	OTUB	coord: 21..299 score: 2.7
IPR019400	Peptidase C65, otubain	PFAM	PF10275	Peptidase_C65	coord: 46..298 score: 9.5
None	No IPR available	PANTHER	PTHR12931	UBIQUITIN THIOLESTERASE PROTEIN OTUB	coord: 32..299 score: 2.7E
None	No IPR available	unknown	SSF54001	Cysteine proteinases	coord: 52..298 score: 1.28

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi07G003890	Csa4G007720	Cucumber (Chinese Long) v2	culsiB312
Lsi07G003890	CSPI04G02320	Wild cucumber (PI 183967)	cpilsiB311
Lsi07G003890	Cucsa.095700	Cucumber (Gy14) v1	cgylsiB113
Lsi07G003890	CmaCh14G013970	Cucurbita maxima (Rimu)	cmalsiB256
Lsi07G003890	CmaCh06G017140	Cucurbita maxima (Rimu)	cmalsiB773
Lsi07G003890	CmoCh06G017050	Cucurbita moschata (Rifu)	cmolsiB764
Lsi07G003890	CmoCh14G014330	Cucurbita moschata (Rifu)	cmolsiB244
Lsi07G003890	Cla012775	Watermelon (97103) v1	lsiwmB438
Lsi07G003890	MELO3C017814	Melon (DHL92) v3.5.1	lsimeB447
Lsi07G003890	ClCG07G011910	Watermelon (Charleston Gray)	lsiwcgB406
Lsi07G003890	MELO3C017814.2	Melon (DHL92) v3.6.1	lsimedB465
Lsi07G003890	Cla97C07G138650	Watermelon (97103) v2	lsiwmbB405
Lsi07G003890	Bhi01G002848	Wax gourd	lsiwgoB558
Lsi07G003890	Cp4.1LG08g06450	Cucurbita pepo (Zucchini)	cpelsiB726
Lsi07G003890	Cp4.1LG03g13380	Cucurbita pepo (Zucchini)	cpelsiB516
Lsi07G003890	CsGy4G002270	Cucumber (Gy14) v2	cgyblsiB285
Lsi07G003890	Carg06550	Silver-seed gourd	carlsiB109
Lsi07G003890	Carg15424	Silver-seed gourd	carlsiB635
Lsi07G003890	CsaV3_4G002250	Cucumber (Chinese Long) v3	cuclsiB331

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None