Cucsa.095700 (gene) Cucumber (Gy14) v1

NameCucsa.095700
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionUbiquitin thioesterase otubain-like
Locationscaffold00919 : 1581117 .. 1587515 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCATAGTTGACCTCTTTTCATCTTCTTCTTCTTCTTCTTCTTCTTCTCCGTCTTCTTCAGCCCTAATCCCTCCCTCCACCACTCCCACTTCTCCTTCATTTCCCATTCCAACGCTCCCTCCATTATTTCCCACCTCAACAATGGTTATCTCTCCCCTCCATGGCGCCCTTTTCCCTTTCCTCTTTTAGTATCTGCTTCCCCCTACCTCTGACAACTCACCACTCACTGTCCTTCATTCCCTTCGTCCTCTGTAAGATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCTATTGATTCTTGCTTATTGTCGGATCTGGATTCTGATTTACCCGTTTCTAGTATGGATGGATTTGAGCTTCGATGTTTGCATCGCTTTCTTGGGTTTACCCTACGTGCATGGGAAATTATTTTTGTGTTTACCGTGTAGGCTGAGGGGATTTAATAATTTTGGATTTCGATATCTTTTTCCTTATAGATTAAGTTCGAATCAGTGATTTATTACACTTTCATTTCCATATTTTAAGTTCCTGTTGATTGCCCTAATTTCTGATGCGAAGTGCTCAATCTAATCACTAATGCACATTGAGAAGCAATGTGTGAAGATGTGCAGTTTCTATCTCGATTTTTTATGTTTTATTTGATGACCGAACTGGAACCTGACTTTTTTTGTGTCCATTATAACTACGAGCAATTTCTAGTTCTTTAGTAGGATAGATTACTTGCTTGCATCACGATCTTTAAGAACTCGGCTAAGAAAGTGGATGCCGACTGTATTAGGTTTTTAAAGCTGTGTTTGTTGCAAGTGGTTTGATATTGATGTGCAATATTTTCTTTCTTTTTTTTATTTTTTTTAATTACTGGCCTGGTCTATATTCACTGGCATTCGATTATCCGTATCATATGGTTTGAGAATGATCGAGTTGTTAACTTGAAAGATGAGAAGAAGAATGCTATTGCTACACGTTTATTTATTGCTAGATAATGTTTTTTCAACGCTCAGCAGGTGGCCTCCTAGTAGGCAAGTTTATGAGCTGGGTGGATCATTACAGTGAAGTTCCAAGGGAAATTCTTGAATAGGTTTTTCAAACCCGACTTGAAACAGAAGTATTTCGTTAAAAGAATGCAGAATCAGGAAAATCTCGATGCAGATGGGAAAGCGGAGTCTGTAATATCCATTCAAACATCAGAATACGATTCCTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTCGGTGACAAGGCATGTTTCACCCAACTAAGTTTTAGTCTCCTGCATTGTCCTGTCTTGATCTTGCCACACTTTTTCCCTCCTCTTCCTTCACGTGTCTGTGTTATTTATGATTCTATGCATGTAGGAAACGGAGTTCTACTCCTTAGTCTTTCTTTCTTTTCCCATAAATTATGCTATGAAGTAGTACTCTTATTTTATTTGCTGCAACATGTTTGGGAAGTTCTCAAAACGTAACCTAGGTTTGGAAATTATTTAGAACTTAGAAATGTTTGTGGGATTCTGCATTGCTTTTTGCTTCAAAAGCCACCCTTTCTGTATGTTTAATGGCGTTTTTGGTGTCTAACCTTTGGGCCTACAGTTCTAACCCTTTTTATCATGTTTTTGTTTGTTCTTAATTCGTATCTTAACAATTGGAGGTCCATCTTATTAGTTATTATTTTTCCTACTTTATTTTGGCTTGGCTCTATTTTTTCATTTTCATTTCCTGAAAGTGAATCTGTATTCTCTGCTTTTTCCACTCTTTTTACTCCTTTTGGAGTTTGTATTCTTTGAGCAATAGTCTCTCTGTTCAAAGTAGTTCTTTCTCTTGTTTTTTAGGGGTGGAAAGGGACTCTTGTGATGTTTGGGCTTGGTGAAGTTTCAAGCTTCCCTTTGGGCTTCAATTTTGAGGACTTTTTGTAATTACTCTTTAGTTTACATTTTACTTTCTTGGTCCACTTTCTCTAGTAGGGCTTTTGTGTGCTTGGTTTTTTGTATGCCCTTGAACTCTTTCACTTTTTCTCTATGAATAGTTTTCCATAAAAAAATTTAATTAAAAAAGAAAGAAGTGAATGACTCTTTGAAAGTTCTTTTTACCTTTTTAACGTATGTTTTGAATGCCTTACAACTGTGGCCTTGTAGTAAGATGTTTTTATCAGTTAATTTTTCTTTTACTCCGTAATGAATCTTAATGGCAATGGTTTTGCAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCGGGAAGTCCAATTTTGCTGGAGAAAATTAAGGTTTTACTTGTTGCTTATTGTTATTTTAGTAGAGTTGTCCTTGGAATAATTATTTATGTTACATGTTATGCAGGTGCTCAGTGGCCAGTATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGAAGCTTCATGTTTTCTTATCTTGTAAGTTTAATTCTTTTAATTTATCTCATTTACTTTGCTATTATACTTTTTGTGCATCATCGAGCATCAGCTGTTACTGACTTGGCCTCTCAAATCAGGAACACATTTTAGAGTCACAAGACAAAACTGAAGTTGATCGCATCAAAACAAATGTTGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACCTTTGAAGATTTTTTTGCGGTAAATTCCTACCATTCCTTTTTTTTTAAAAAAAACAATTAATCTATTGCACATTATCGCCCTATGCTCTAAGCAGTATGGCTTATTTCTTCCATTCAACTGGAATTGGTGGAAGTTGAGAACTATTCATACTAGTCTCTAAATGTGTTATTGAACATACCATATATAAATATTGTGTAAGATACTTAATTAGGGAGATTGGTATGGTATTAGTAAGGGGCATAAGGGCAATTAGATTGGGAGTTGATGTATCTTGTTATAAATAGGAGAGTCAGTGAGGGAAGGTGCCCAGCAATTGTCGAGTGTTATAGGGCTTGGGATGCCATACTCAAGAGGAAGGTTCCAAGTGCCTTATATTGGATTATCTCGTAGTTCCATCTTTATCAATTTTATTGTTGTTGAGATGTTTTCTTTGCAGTTTGTGATGGTATAGTAGGTTTATTTTCCCTTTTTTGAACCTTTCCATCTAAGTATGTGCTTTCTCTCCAATTGATGGATATGAATGAGGAAATTAGACCAAACTAAAGAGGACTATAGGGGGCTGCAAGTTCAATGATGGAAAGGTGGTGATGAGAATCTATTAATGAAATTGGCCCCTCCACTTTGTAATTTCATCCATTAATGAAATTGTCTCTTAACAAAAAGAAAAAAAGTGGTGATGAGAATCTATTTTTTGTGATGCCAGATTTAACATTAACGTCATTGCAATGACATCTCATATTGAATTAACTTTGAATACGAACATTTTGTATATTATGTCTTTACCTCATCGAAGTCCAGTCTTTTTCATAACTCATATATAGACTCTAGAGCTATTCTTTAAAAATGTGTGGACCAACTTTTAAATACATTGTTCCAGATTTATACTACCTTAAAGATGTCTAGAGCCTAGACTCGTCCTTCTTTATTATTGCTGTCTTTGTTTCATTTTGGTATAAACTGGCTAGATATCCTTGGTGGTTAATGATGGGTGCCATAAATATAAATTATTTATTAATAAATATGTTTAAAATATCTTTACAGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAATCCTCTATAAGGTGTCAATGACCTGCCCTTCTACCTTGGAATTTTGATTCCTGAATTTTTAGTTGATAGTGCAATTTTCTGTTGCCATCAATTATGAAATTTTGCCATTCATGATTGCATTTGACTCTTATTTTTCTTGGATGCAGTCATGAAGAACTTGTTATTAGGAGTCGGGACCAGTCTATCTCTGATTATGGTCAGTGATGTTTTATATTCAATATTGAATTCTCTCTTTCCATTTCTACTGACAAAATTGTTTTTCTAGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCAGAATTTTTTGAGCCTTTTATTATGGGATTGACAAATGGAACGGTTGATCAGGTTTCCTAAATTTTTAACCCCTATAAGTTTTGCATTATGTTTTGACTTTTATCTTTAGATTTATGTTGGAAATTCAACCCTACAAGATATTTGAAATCTAAGCTGATTTGGCTAGGTGAACCCTTTTACAAGTGTAGTTTCAAGCAGAAGCAGCTAATTGTTATAAAGCAATAATGATGATTTAGACCATGTATACCTTGGACAGGAATAAGAATGGATATTTACGATTTCCGCTCTTGTTTACTTCTTCTTGGGATTGGAATAAGTATAACCCACTTAAGAATGTAGAACCTTGCTCTTATTTTGGAGCAACATCTTTACTTCATAATAGATAACCATTCTCTCTCCTATTGCTGTTCTATGGGTTCAAAGTCTTTTCTGTGGGTCTGTTGACCTTTTTTCCTCATTCTAAAAATCTAAATGTTAACAAACAATATCGTATCAACCACCGTTTTCTTATTCCAGACTTCATAATAATTGTAGTAAACAAAGTTTCAGAAATTCTATTTCAAATCCTTTTTTTTTTTTCTATTCAATCAGACATGATGGATTGCCATATGGTTTTATTCCCTCATTAAATAAACTAAGCCTTGGGTTCATGATTTTATCATCACATTTATATTTAAATGAAATCACTTTAGCCTTATTATTTCCCTTTTTTTGAGTGCACATGTGGGGATTTAAACTTTTCAACCTTGTTTCCATTTCAAAAGAAAAAAAAAAATTTAAACTTTTGACCTTTTGGTCTGCTATTATTCCCTTTTTGGTGGATCAGAATCCCAAACAACAGTAAGCCTTGGTCTTTGTGTTTGGGGGTTAACTTTTCCAAGGGTTGGTCACTTATGATGATAATTTTTAGTTTTTGGTTTGGTGGGGAAACTAGACATAGAC

mRNA sequence

ACCATAGTTGACCTCTTttcatcttcttcttcttcttcttcttcttctccgtcttcttcAGCCCTAATCCCTCCCTCCACCACTCCCACTTCTCCTTCATTTCCCATTCCAACGCTCCCTCCATTATTTCCCACCTCAACAATGGTTATCTCTCCCCTCCATGGCGCCCTTTTCCCTTTCCTCTTTTAGTATCTGCTTCCCCCTACCTCTGACAACTCACCACTCACTGTCCTTCATTCCCTTCGTCCTCTGTGGCCTCCTAGTAGGCAAGTTTATGAGCTGGGTGGATCATTACAGTGAAGTTCCAAGGGAAATTCTTGAATAGGTTTTTCAAACCCGACTTGAAACAGAAGTATTTCGTTAAAAGAATGCAGAATCAGGAAAATCTCGATGCAGATGGGAAAGCGGAGTCTGTAATATCCATTCAAACATCAGAATACGATTCCTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTCGGTGACAAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCGGGAAGTCCAATTTTGCTGGAGAAAATTAAGGTGCTCAGTGGCCAGTATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGAAGCTTCATGTTTTCTTATCTTGAACACATTTTAGAGTCACAAGACAAAACTGAAGTTGATCGCATCAAAACAAATGTTGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACCTTTGAAGATTTTTTTGCGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAATCCTCTATAAGTCATGAAGAACTTGTTATTAGGAGTCGGGACCAGTCTATCTCTGATTATGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCAGAATTTTTTGAGCCTTTTATTATGGGATTGACAAATGGAACGGTTGATCAGGTTTCCTAAATTTTTAACCCCTATAAGTTTTGCATTATGTTTTGACTTTTATCTTTAGATTTATGTTGGAAATTCAACCCTACAAGATATTTGAAATCTAAGCTGATTTGGCTAGGTGAACCCTTTTACAAGTGTAGTTTCAAGCAGAAGCAGCTAATTGTTATAAAGCAATAATGATGATTTAGACCATGTATACCTTGGACAGGAATAAGAATGGATATTTACGATTTCCGCTCTTGTTTACTTCTTCTTGGGATTGGAATAAGTATAACCCACTTAAGAATGTAGAACCTTGCTCTTATTTTGGAGCAACATCTTTACTTCATAATAGATAACCATTCTCTCTCCTATTGCTGTTCTATGGGTTCAAAGTCTTTTCTGTGGGTCTGTTGACCTTTTTTCCTCATTCTAAAAATCTAAATGTTAACAAACAATATCGTATCAACCACCGTTTTCTTATTCCAGACTTCATAATAATTGTAGTAAACAAAGTTTCAGAAATTCTATTTCAAATCCTTTTTTTTTTTTCTATTCAATCAGACATGATGGATTGCCATATGGTTTTATTCCCTCATTAAATAAACTAAGCCTTGGGTTCATGATTTTATCATCACATTTATATTTAAATGAAATCACTTTAGCCTTATTATTTCCCTTTTTTTGAGTGCACATGTGGGGATTTAAACTTTTCAACCTTGTTTCCATTTCAAAAGAAAAAAAAAAATTTAAACTTTTGACCTTTTGGTCTGCTATTATTCCCTTTTTGGTGGATCAGAATCCCAAACAACAGTAAGCCTTGGTCTTTGTGTTTGGGGGTTAACTTTTCCAAGGGTTGGTCACTTATGATGATAATTTTTAGTTTTTGGTTTGGTGGGGAAACTAGACATAGAC

Coding sequence (CDS)

ATGCAGAATCAGGAAAATCTCGATGCAGATGGGAAAGCGGAGTCTGTAATATCCATTCAAACATCAGAATACGATTCCTGGGGCAATTTGGGTGATGATGATATCATGCTGCAGAAATCTGCAATTTTTGTTCAGGAAGCTGAGAAAGTTCCATTTGTCGGTGACAAGGAACCACTTTCTTCTTTAGAAGCTGAATATAAATCGGGAAGTCCAATTTTGCTGGAGAAAATTAAGGTGCTCAGTGGCCAGTATGCTGCCATTCGTCGAACACGTGGTGATGGGAACTGCTTTTTCCGAAGCTTCATGTTTTCTTATCTTGAACACATTTTAGAGTCACAAGACAAAACTGAAGTTGATCGCATCAAAACAAATGTTGAAAATTGTAGGAAAACACTTCGAAGTCTGGGATATACAGAATTTACCTTTGAAGATTTTTTTGCGCTATTCCTTGAGCAGCTGGAAAGTGCTCTTCAAGGAAATGAATCCTCTATAAGTCATGAAGAACTTGTTATTAGGAGTCGGGACCAGTCTATCTCTGATTATGTTGTTATGTTTTTCAGGTTTGTTACATCAGGTGAAATACAAAAGCGTTCAGAATTTTTTGAGCCTTTTATTATGGGATTGACAAATGGAACGGTTGATCAGGTTTCCTAA

Protein sequence

MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQVS*
BLAST of Cucsa.095700 vs. Swiss-Prot
Match: OTUBL_ARATH (Ubiquitin thioesterase otubain-like OS=Arabidopsis thaliana GN=At1g28120 PE=2 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 4.9e-117
Identity = 211/302 (69.87%), Postives = 245/302 (81.13%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ ++  D +AE   SI   + + WGN    +    + +   +EA KVP+VGDKEPLS
Sbjct: 1   MQNQIDMVKD-EAEVAASISAIKGEEWGNCSSVE---DQPSFQEEEAAKVPYVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEY+SGSPILLEKIK+L  QY  IRRTRGDGNCFFRSFMFSYLEHILESQD+ EVDR
Sbjct: 61  SLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL++LGYT+FTFEDFFALFLEQL+  LQG E SIS++ELV RSRDQS+SD
Sbjct: 121 IKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           Y+VMFFRFVT+G+I+ R++FFEPFI GL+N TVDQFCK++VEPMGEESDH+HI ALSDAL
Sbjct: 181 YIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GV IRV+YLDRSSCDSGG++VNHHDFVP      +     E   PFITLLYRPGHYDILY
Sbjct: 241 GVAIRVVYLDRSSCDSGGVTVNHHDFVPVG---ITNEKDEEASAPFITLLYRPGHYDILY 295

Query: 301 PK 303
           PK
Sbjct: 301 PK 295

BLAST of Cucsa.095700 vs. Swiss-Prot
Match: OTUB1_HUMAN (Ubiquitin thioesterase OTUB1 OS=Homo sapiens GN=OTUB1 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-47
Identity = 122/299 (40.80%), Postives = 168/299 (56.19%), Query Frame = 1

Query: 2   QNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLSS 61
           Q QE L +D +  + ++             D+ IM Q+  I  + A + P V ++  LS 
Sbjct: 9   QKQEPLGSDSEGVNCLAY------------DEAIMAQQDRIQQEIAVQNPLVSERLELSV 68

Query: 62  LEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDRI 121
           L  EY     I  +KIK L  +Y+ IR+TR DGNCF+R+F FS+LE +L+  D  E+ R 
Sbjct: 69  LYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLD--DSKELQRF 128

Query: 122 KTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISDY 181
           K      ++ L S G+TEFT EDF   F++ +E      E   S  +L+    DQS SDY
Sbjct: 129 KAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQV----EKQTSVADLLASFNDQSTSDY 188

Query: 182 VVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALG 241
           +V++ R +TSG +Q+ S+FFE FI G    TV +FC+  VEPM +ESDH+HIIAL+ AL 
Sbjct: 189 LVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQEVEPMCKESDHIHIIALAQALS 248

Query: 242 VPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 301
           V I+V Y+DR      G + N H F P  +E            P + LLYRPGHYDILY
Sbjct: 249 VSIQVEYMDRGE----GGTTNPHIF-PEGSE------------PKVYLLYRPGHYDILY 270

BLAST of Cucsa.095700 vs. Swiss-Prot
Match: OTUB1_MOUSE (Ubiquitin thioesterase OTUB1 OS=Mus musculus GN=Otub1 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-47
Identity = 122/299 (40.80%), Postives = 168/299 (56.19%), Query Frame = 1

Query: 2   QNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLSS 61
           Q QE L +D +  + ++             D+ IM Q+  I  + A + P V ++  LS 
Sbjct: 9   QKQEPLGSDSEGVNCLAY------------DEAIMAQQDRIQQEIAVQNPLVSERLELSV 68

Query: 62  LEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDRI 121
           L  EY     I  +KIK L  +Y+ IR+TR DGNCF+R+F FS+LE +L+  D  E+ R 
Sbjct: 69  LYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLD--DSKELQRF 128

Query: 122 KTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISDY 181
           K      ++ L S G+TEFT EDF   F++ +E      E   S  +L+    DQS SDY
Sbjct: 129 KAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQV----EKQTSVADLLASFNDQSTSDY 188

Query: 182 VVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALG 241
           +V++ R +TSG +Q+ S+FFE FI G    TV +FC+  VEPM +ESDH+HIIAL+ AL 
Sbjct: 189 LVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQEVEPMCKESDHIHIIALAQALS 248

Query: 242 VPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 301
           V I+V Y+DR      G + N H F P  +E            P + LLYRPGHYDILY
Sbjct: 249 VSIQVEYMDRGE----GGTTNPHVF-PEGSE------------PKVYLLYRPGHYDILY 270

BLAST of Cucsa.095700 vs. Swiss-Prot
Match: OTUB1_RAT (Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus GN=Otub1 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-47
Identity = 122/299 (40.80%), Postives = 168/299 (56.19%), Query Frame = 1

Query: 2   QNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLSS 61
           Q QE L +D +  + ++             D+ IM Q+  I  + A + P V ++  LS 
Sbjct: 9   QKQEPLGSDSEGVNCLAY------------DEAIMAQQDRIQQEIAVQNPLVSERLELSV 68

Query: 62  LEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDRI 121
           L  EY     I  +KIK L  +Y+ IR+TR DGNCF+R+F FS+LE +L+  D  E+ R 
Sbjct: 69  LYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLD--DSKELQRF 128

Query: 122 KTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISDY 181
           K      ++ L S G+TEFT EDF   F++ +E      E   S  +L+    DQS SDY
Sbjct: 129 KAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQV----EKQTSVADLLASFNDQSTSDY 188

Query: 182 VVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDALG 241
           +V++ R +TSG +Q+ S+FFE FI G    TV +FC+  VEPM +ESDH+HIIAL+ AL 
Sbjct: 189 LVVYLRLLTSGYLQRESKFFEHFIEG--GRTVKEFCQQEVEPMCKESDHIHIIALAQALS 248

Query: 242 VPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 301
           V I+V Y+DR      G + N H F P  +E            P + LLYRPGHYDILY
Sbjct: 249 VSIQVEYMDRGE----GGTTNPHVF-PEGSE------------PKVYLLYRPGHYDILY 270

BLAST of Cucsa.095700 vs. Swiss-Prot
Match: OTUBL_DROME (Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster GN=CG4968 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.7e-45
Identity = 114/272 (41.91%), Postives = 158/272 (58.09%), Query Frame = 1

Query: 32  DDDIMLQKSAIFVQEAEKVPFVGDKEPLSSLEAEYKSGSPILLEKIKVLSGQYAAIRRTR 91
           D+ I+ QK  I  + ++  P V ++ PL+ L AEY SG  I   KI+ LS +Y  IRRTR
Sbjct: 12  DELIIQQKRDIEKEISDTTPLVSEQLPLTCLYAEY-SGDEIFTAKIQDLSKKYKFIRRTR 71

Query: 92  GDGNCFFRSFMFSYLEHILESQDKTEVDRIKTNVENCRKTLRSLGYTEFTFEDFFALFLE 151
            DGNCFFR+F +SYLE+++   + +     K   E  ++ L  LG+  FT EDF   F+E
Sbjct: 72  PDGNCFFRAFAYSYLEYLI--SNTSAYQEFKKLAEESKEKLVQLGFPSFTLEDFHETFME 131

Query: 152 QLESALQGNES--SISHEELVIRSRDQSISDYVVMFFRFVTSGEIQKRSEFFEPFIMGLT 211
            ++     N    S   +EL     +Q  SDYVV++ R +TSG++Q+ ++F++ FI G  
Sbjct: 132 VIQRVSPDNAGGHSTVQDELHKIFNEQGYSDYVVVYLRLITSGKLQEEADFYQNFIEG-- 191

Query: 212 NGTVDQFCKTAVEPMGEESDHVHIIALSDALGVPIRVLYLDRSSCDSGGLSVNHHDFVPA 271
           + T++ F    VEPM +ESDH+HIIAL  ALG  +RV YLDR      G +V  HDF P 
Sbjct: 192 DLTIEAFRHLEVEPMYKESDHIHIIALCTALGAGVRVEYLDRGE----GGTVKAHDF-PE 251

Query: 272 TTEVASGSAASEIKIPFITLLYRPGHYDILYP 302
            +E            P I L+YRPGHYDILYP
Sbjct: 252 GSE------------PRIYLIYRPGHYDILYP 261

BLAST of Cucsa.095700 vs. TrEMBL
Match: A0A0A0KX37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007720 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.1e-168
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300

Query: 301 PK 303
           PK
Sbjct: 301 PK 302

BLAST of Cucsa.095700 vs. TrEMBL
Match: M5XZ09_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007269mg PE=4 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 1.3e-135
Identity = 236/302 (78.15%), Postives = 268/302 (88.74%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ +  DG+ ES  S+ T E+D W N GD DIM Q SAI  +EAEK+PF+GDKEPLS
Sbjct: 74  MQNQDGVQVDGEVESATSVPTPEFDEWANFGDQDIMQQHSAIRAEEAEKIPFLGDKEPLS 133

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 134 SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 193

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE+SISH+EL++RSRDQS+SD
Sbjct: 194 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEASISHDELILRSRDQSVSD 253

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEI+KR+EFFEPFI+GLTN TV+QFCK++VEPMGEESDHVHI ALSDAL
Sbjct: 254 YVVMFFRFVTSGEIRKRAEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 313

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRV+YLDRSSCD+GG+SVNHHDFVPA +++ + S  SE   PFITLLYRPGHYDILY
Sbjct: 314 GVPIRVVYLDRSSCDTGGVSVNHHDFVPAGSDLPNASGCSEKVSPFITLLYRPGHYDILY 373

Query: 301 PK 303
           PK
Sbjct: 374 PK 375

BLAST of Cucsa.095700 vs. TrEMBL
Match: A0A061E5K2_THECC (Otubain, Ubiquitin thioesterase Otubain, Peptidase C65, otubain isoform 1 OS=Theobroma cacao GN=TCM_008341 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.6e-130
Identity = 233/303 (76.90%), Postives = 264/303 (87.13%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQN+E + ADG+ ES +SI  SE+D W    DDDIM Q+SA   +EA+KVPFVGDKEPL+
Sbjct: 1   MQNREGVVADGEKESAVSIPVSEFDDWAKFADDDIMQQQSAFHAEEAKKVPFVGDKEPLT 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
            L AEY+SGSPILLEKIK+L  QYAAIRRTRGDGNCFFRSFMFSYLE++LESQD  EVDR
Sbjct: 61  MLAAEYESGSPILLEKIKLLGQQYAAIRRTRGDGNCFFRSFMFSYLEYVLESQDHAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE SIS +EL+IRSRDQSISD
Sbjct: 121 IKGNVEECRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEDSISQDELIIRSRDQSISD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEI+KRSEFFEPFI+GLTN TV+QFCK++VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASG-SAASEIKIPFITLLYRPGHYDIL 300
           GVPIRV+YLDRSSCD+GG+SVNHHDFVP +   ++  S ++E   PFITLLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDNGGVSVNHHDFVPTSGGHSNATSGSTEPVSPFITLLYRPGHYDIL 300

Query: 301 YPK 303
           YPK
Sbjct: 301 YPK 303

BLAST of Cucsa.095700 vs. TrEMBL
Match: A0A0D2PW37_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G197000 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 2.1e-130
Identity = 232/303 (76.57%), Postives = 262/303 (86.47%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+   AD + ES +SI  SE D W N  DDDIM Q+SAI  +EA+K+PFVGDKE LS
Sbjct: 1   MQNQDGAVADREKESAVSIPISEVDDWANFADDDIMQQQSAIHAEEAKKIPFVGDKEALS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
            L AEY+SGSPILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD+ EVDR
Sbjct: 61  MLAAEYESGSPILLEKIKVLDQQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL+SLG+T+FTFEDFF+LFLEQLE  LQGNE SIS +EL++RSRDQSISD
Sbjct: 121 IKGNVEECRKTLQSLGHTDFTFEDFFSLFLEQLECVLQGNEDSISQDELILRSRDQSISD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEI+KRSEFFEPFI+GLTN TV+QFCK++VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKI-PFITLLYRPGHYDIL 300
           GVPIRV+YLDRSSCD GG+SVNHHDF+P + + ++    S + + PFITLLYRPGHYDIL
Sbjct: 241 GVPIRVVYLDRSSCDIGGVSVNHHDFLPTSGDKSNAKGGSTVPVKPFITLLYRPGHYDIL 300

Query: 301 YPK 303
           YPK
Sbjct: 301 YPK 303

BLAST of Cucsa.095700 vs. TrEMBL
Match: A0A0S3S2C8_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G032700 PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.7e-127
Identity = 226/302 (74.83%), Postives = 258/302 (85.43%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQ++E +  DG+  S +++  +E+D W N GDDDIM Q+ +I   EA+KVPFVGDKEPLS
Sbjct: 1   MQSKEAVVEDGEITSSVTVPDTEFDEWNNFGDDDIMQQQYSIQADEAKKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEYKSGSPILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHI+E QD+ EVDR
Sbjct: 61  SLAAEYKSGSPILLEKIKVLDEQYAAIRRTRGDGNCFFRSFMFSYLEHIMECQDQAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           I+ NVE  RK L++LGY + TFEDFFALFLEQLE  +QG E+SISHEELVIRSRDQSISD
Sbjct: 121 IQPNVEKSRKALQTLGYADLTFEDFFALFLEQLECVIQGKETSISHEELVIRSRDQSISD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTS EIQ R+EFFEPFI+GLTN TV+QFCK +VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSAEIQMRTEFFEPFILGLTNTTVEQFCKLSVEPMGEESDHVHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRV+YLDRSSCD+GG+SVNHHDF+P   ++ + S +S   IPFITLLYRPGHYDILY
Sbjct: 241 GVPIRVVYLDRSSCDTGGVSVNHHDFMPVAGDLPNASCSSVKNIPFITLLYRPGHYDILY 300

Query: 301 PK 303
           PK
Sbjct: 301 PK 302

BLAST of Cucsa.095700 vs. TAIR10
Match: AT1G28120.1 (AT1G28120.1 Ovarian tumour, otubain (InterPro:IPR003323), Ubiquitin thioesterase Otubain (InterPro:IPR016615), Peptidase C65, otubain (InterPro:IPR019400))

HSP 1 Score: 422.2 bits (1084), Expect = 2.8e-118
Identity = 211/302 (69.87%), Postives = 245/302 (81.13%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ ++  D +AE   SI   + + WGN    +    + +   +EA KVP+VGDKEPLS
Sbjct: 1   MQNQIDMVKD-EAEVAASISAIKGEEWGNCSSVE---DQPSFQEEEAAKVPYVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEY+SGSPILLEKIK+L  QY  IRRTRGDGNCFFRSFMFSYLEHILESQD+ EVDR
Sbjct: 61  SLAAEYQSGSPILLEKIKILDSQYIGIRRTRGDGNCFFRSFMFSYLEHILESQDRAEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL++LGYT+FTFEDFFALFLEQL+  LQG E SIS++ELV RSRDQS+SD
Sbjct: 121 IKVNVEKCRKTLQNLGYTDFTFEDFFALFLEQLDDILQGTEESISYDELVNRSRDQSVSD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           Y+VMFFRFVT+G+I+ R++FFEPFI GL+N TVDQFCK++VEPMGEESDH+HI ALSDAL
Sbjct: 181 YIVMFFRFVTAGDIRTRADFFEPFITGLSNATVDQFCKSSVEPMGEESDHIHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GV IRV+YLDRSSCDSGG++VNHHDFVP      +     E   PFITLLYRPGHYDILY
Sbjct: 241 GVAIRVVYLDRSSCDSGGVTVNHHDFVPVG---ITNEKDEEASAPFITLLYRPGHYDILY 295

Query: 301 PK 303
           PK
Sbjct: 301 PK 295

BLAST of Cucsa.095700 vs. NCBI nr
Match: gi|449468900|ref|XP_004152159.1| (PREDICTED: ubiquitin thioesterase otubain-like [Cucumis sativus])

HSP 1 Score: 600.5 bits (1547), Expect = 1.6e-168
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300

Query: 301 PK 303
           PK
Sbjct: 301 PK 302

BLAST of Cucsa.095700 vs. NCBI nr
Match: gi|659108333|ref|XP_008454143.1| (PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo])

HSP 1 Score: 592.8 bits (1527), Expect = 3.4e-166
Identity = 297/302 (98.34%), Postives = 300/302 (99.34%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+NLD DGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS
Sbjct: 1   MQNQDNLDTDGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR
Sbjct: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISH+ELVIRSRDQSISD
Sbjct: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHDELVIRSRDQSISD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL
Sbjct: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEV+S SAASEIKIPFITLLYRPGHYDILY
Sbjct: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVSSDSAASEIKIPFITLLYRPGHYDILY 300

Query: 301 PK 303
           PK
Sbjct: 301 PK 302

BLAST of Cucsa.095700 vs. NCBI nr
Match: gi|657956293|ref|XP_008369628.1| (PREDICTED: ubiquitin thioesterase otubain-like [Malus domestica])

HSP 1 Score: 493.4 bits (1269), Expect = 2.8e-136
Identity = 238/302 (78.81%), Postives = 266/302 (88.08%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ +  DG+ ES  S+ T E+D W N GD DIMLQ SAI  +EAEK+PFVGDKEPLS
Sbjct: 1   MQNQDGVQVDGEVESAASVATPEFDEWANFGDQDIMLQHSAIRAEEAEKIPFVGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 61  SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE SISH+EL++RSRDQS+SD
Sbjct: 121 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEESISHDELILRSRDQSVSD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEI+KRSEFFEPFI+GLTN TV+QFCK++VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRSEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRV+YLDRSSCD+GG+SVNHHDF+PA ++    S  SE   PFITLLYRPGHYDILY
Sbjct: 241 GVPIRVVYLDRSSCDNGGVSVNHHDFIPAGSDAPKASEGSEKVSPFITLLYRPGHYDILY 300

Query: 301 PK 303
           PK
Sbjct: 301 PK 302

BLAST of Cucsa.095700 vs. NCBI nr
Match: gi|596182099|ref|XP_007223296.1| (hypothetical protein PRUPE_ppa007269mg [Prunus persica])

HSP 1 Score: 490.7 bits (1262), Expect = 1.8e-135
Identity = 236/302 (78.15%), Postives = 268/302 (88.74%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ +  DG+ ES  S+ T E+D W N GD DIM Q SAI  +EAEK+PF+GDKEPLS
Sbjct: 74  MQNQDGVQVDGEVESATSVPTPEFDEWANFGDQDIMQQHSAIRAEEAEKIPFLGDKEPLS 133

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 134 SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 193

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S LQGNE+SISH+EL++RSRDQS+SD
Sbjct: 194 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLQGNEASISHDELILRSRDQSVSD 253

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEI+KR+EFFEPFI+GLTN TV+QFCK++VEPMGEESDHVHI ALSDAL
Sbjct: 254 YVVMFFRFVTSGEIRKRAEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 313

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRV+YLDRSSCD+GG+SVNHHDFVPA +++ + S  SE   PFITLLYRPGHYDILY
Sbjct: 314 GVPIRVVYLDRSSCDTGGVSVNHHDFVPAGSDLPNASGCSEKVSPFITLLYRPGHYDILY 373

Query: 301 PK 303
           PK
Sbjct: 374 PK 375

BLAST of Cucsa.095700 vs. NCBI nr
Match: gi|645231113|ref|XP_008222244.1| (PREDICTED: ubiquitin thioesterase otubain-like [Prunus mume])

HSP 1 Score: 488.8 bits (1257), Expect = 6.8e-135
Identity = 235/302 (77.81%), Postives = 267/302 (88.41%), Query Frame = 1

Query: 1   MQNQENLDADGKAESVISIQTSEYDSWGNLGDDDIMLQKSAIFVQEAEKVPFVGDKEPLS 60
           MQNQ+ +  DG+ ES  S+ T E+D W N GD DIM Q SAI  +EAEK+PF+GDKEPLS
Sbjct: 1   MQNQDGVQVDGEVESATSVPTPEFDEWANFGDQDIMQQHSAIRAEEAEKIPFLGDKEPLS 60

Query: 61  SLEAEYKSGSPILLEKIKVLSGQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDKTEVDR 120
           SL AEY+SGS ILLEKIKVL  QYAAIRRTRGDGNCFFRSFMFSYLEHILESQD++EVDR
Sbjct: 61  SLAAEYQSGSAILLEKIKVLGEQYAAIRRTRGDGNCFFRSFMFSYLEHILESQDQSEVDR 120

Query: 121 IKTNVENCRKTLRSLGYTEFTFEDFFALFLEQLESALQGNESSISHEELVIRSRDQSISD 180
           IK NVE CRKTL+SLGY +FTFEDFFALFLEQL+S L GNE+SISH+EL++RSRDQS+SD
Sbjct: 121 IKANVEQCRKTLQSLGYADFTFEDFFALFLEQLDSVLHGNEASISHDELILRSRDQSVSD 180

Query: 181 YVVMFFRFVTSGEIQKRSEFFEPFIMGLTNGTVDQFCKTAVEPMGEESDHVHIIALSDAL 240
           YVVMFFRFVTSGEI+KR+EFFEPFI+GLTN TV+QFCK++VEPMGEESDHVHI ALSDAL
Sbjct: 181 YVVMFFRFVTSGEIRKRAEFFEPFILGLTNATVEQFCKSSVEPMGEESDHVHITALSDAL 240

Query: 241 GVPIRVLYLDRSSCDSGGLSVNHHDFVPATTEVASGSAASEIKIPFITLLYRPGHYDILY 300
           GVPIRV+YLDRSSCD+GG+SVNHHDFVPA +++ + S  SE   PFITLLYRPGHYDILY
Sbjct: 241 GVPIRVVYLDRSSCDTGGVSVNHHDFVPAGSDLPNASGGSEKVSPFITLLYRPGHYDILY 300

Query: 301 PK 303
           PK
Sbjct: 301 PK 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
OTUBL_ARATH4.9e-11769.87Ubiquitin thioesterase otubain-like OS=Arabidopsis thaliana GN=At1g28120 PE=2 SV... [more]
OTUB1_HUMAN2.4e-4740.80Ubiquitin thioesterase OTUB1 OS=Homo sapiens GN=OTUB1 PE=1 SV=2[more]
OTUB1_MOUSE2.4e-4740.80Ubiquitin thioesterase OTUB1 OS=Mus musculus GN=Otub1 PE=1 SV=2[more]
OTUB1_RAT2.4e-4740.80Ubiquitin thioesterase OTUB1 OS=Rattus norvegicus GN=Otub1 PE=1 SV=1[more]
OTUBL_DROME1.7e-4541.91Ubiquitin thioesterase otubain-like OS=Drosophila melanogaster GN=CG4968 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0KX37_CUCSA1.1e-168100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G007720 PE=4 SV=1[more]
M5XZ09_PRUPE1.3e-13578.15Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007269mg PE=4 SV=1[more]
A0A061E5K2_THECC1.6e-13076.90Otubain, Ubiquitin thioesterase Otubain, Peptidase C65, otubain isoform 1 OS=The... [more]
A0A0D2PW37_GOSRA2.1e-13076.57Uncharacterized protein OS=Gossypium raimondii GN=B456_005G197000 PE=4 SV=1[more]
A0A0S3S2C8_PHAAN3.7e-12774.83Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G032700 PE=... [more]
Match NameE-valueIdentityDescription
AT1G28120.12.8e-11869.87 Ovarian tumour, otubain (InterPro:IPR003323), Ubiquitin thioesterase... [more]
Match NameE-valueIdentityDescription
gi|449468900|ref|XP_004152159.1|1.6e-168100.00PREDICTED: ubiquitin thioesterase otubain-like [Cucumis sativus][more]
gi|659108333|ref|XP_008454143.1|3.4e-16698.34PREDICTED: ubiquitin thioesterase otubain-like [Cucumis melo][more]
gi|657956293|ref|XP_008369628.1|2.8e-13678.81PREDICTED: ubiquitin thioesterase otubain-like [Malus domestica][more]
gi|596182099|ref|XP_007223296.1|1.8e-13578.15hypothetical protein PRUPE_ppa007269mg [Prunus persica][more]
gi|645231113|ref|XP_008222244.1|6.8e-13577.81PREDICTED: ubiquitin thioesterase otubain-like [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003323OTU_dom
IPR016615Otubain
IPR019400Peptidase_C65_otubain
IPR003323OTU_dom
IPR016615Otubain
IPR019400Peptidase_C65_otubain
IPR003323OTU_dom
IPR019400Peptidase_C65_otubain
IPR003323OTU_dom
IPR019400Peptidase_C65_otubain
Vocabulary: Biological Process
TermDefinition
GO:0016579protein deubiquitination
GO:0016579protein deubiquitination
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO:0016787hydrolase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
biological_process GO:0016579 protein deubiquitination
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0008233 peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.095700.4Cucsa.095700.4mRNA
Cucsa.095700.3Cucsa.095700.3mRNA
Cucsa.095700.2Cucsa.095700.2mRNA
Cucsa.095700.1Cucsa.095700.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003323OTU domainPROFILEPS50802OTUcoord: 85..217
score: 9
IPR019400Peptidase C65, otubainPFAMPF10275Peptidase_C65coord: 46..214
score: 1.7
NoneNo IPR availablePANTHERPTHR12931UBIQUITIN THIOLESTERASE PROTEIN OTUBcoord: 32..215
score: 4.7
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 52..207
score: 1.86