Lsi06G006840 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi06G006840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionMatrix metalloproteinase, putative
Locationchr06 : 10781287 .. 10782231 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATAAACCTTTGGTTCTTCTCTTTATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCTCCAAGTTTCTCCATTTGAATTTCTCAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAACTTAAGAACTTTTTCTGTTATTACGGTTATTTAAACCATCGAACCAACGCCACTGGTCATCTGATCGAGGCTGACGTTGACGACACCTTCGACGATCTCCTCGAGTCAGCCATTAAAACCTACCAACAATACTTCCATCTTAGCCCCACTGGATTCCTGAATGCCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGCGTTCCAGATATCGTCAATGGAACTACCGATCGAATGCTATCAGAACACAACGACAACGACGGTGATCATGATCACCATCACCACCACCTTCCTCACGTCGTCTCGCACTATGCCTTCTTCCCAGGAAGGCGCAGGTGGCCATCTACCAAATACCATCTCACCTACGCGTTTCTTCCCGGCACTCGAGCAGACGCGAAAGCACCGGTGGCTCGAGCGTTCGCGACGTGGGCTCGAAACACTCATTTTAAGTTTTCTTTGGCTACAAACTATAAAAGAGCTGACTTGAAGATTGGTTTCTATAGAGGAAACCATGGAGATGGATACCCATTTGATGGGCCAGGTGGGACTTTGGCACATGCATTTGCTCCAACTGATGGGAGATTTCATTATGATTCTACAGAGAAATGGGGAGTTGGGGCAGTGAGAGGGCGATATGATTTGCAAACGGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGACATAGCACTGTTAAGAATGCTATAATGTATCCTTATATCAATTCAGGGACTACAAAGGGTTTGAATGTTGATGATATCAAAGGAATCAAGGTTTTATACAATAGACACTAG

mRNA sequence

ATGGCAAATAAACCTTTGGTTCTTCTCTTTATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCTCCAAGTTTCTCCATTTGAATTTCTCAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAACTTAAGAACTTTTTCTGTTATTACGGTTATTTAAACCATCGAACCAACGCCACTGGTCATCTGATCGAGGCTGACGTTGACGACACCTTCGACGATCTCCTCGAGTCAGCCATTAAAACCTACCAACAATACTTCCATCTTAGCCCCACTGGATTCCTGAATGCCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGCGTTCCAGATATCGTCAATGGAACTACCGATCGAATGCTATCAGAACACAACGACAACGACGGTGATCATGATCACCATCACCACCACCTTCCTCACGTCGTCTCGCACTATGCCTTCTTCCCAGGAAGGCGCAGGTGGCCATCTACCAAATACCATCTCACCTACGCGTTTCTTCCCGGCACTCGAGCAGACGCGAAAGCACCGGTGGCTCGAGCGTTCGCGACGTGGGCTCGAAACACTCATTTTAAGTTTTCTTTGGCTACAAACTATAAAAGAGCTGACTTGAAGATTGGTTTCTATAGAGGAAACCATGGAGATGGATACCCATTTGATGGGCCAGGTGGGACTTTGGCACATGCATTTGCTCCAACTGATGGGAGATTTCATTATGATTCTACAGAGAAATGGGGAGTTGGGGCAGTGAGAGGGCGATATGATTTGCAAACGGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGACATAGCACTGTTAAGAATGCTATAATGTATCCTTATATCAATTCAGGGACTACAAAGGGTTTGAATGTTGATGATATCAAAGGAATCAAGGTTTTATACAATAGACACTAG

Coding sequence (CDS)

ATGGCAAATAAACCTTTGGTTCTTCTCTTTATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGCTCCAAGTTTCTCCATTTGAATTTCTCAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAACTTAAGAACTTTTTCTGTTATTACGGTTATTTAAACCATCGAACCAACGCCACTGGTCATCTGATCGAGGCTGACGTTGACGACACCTTCGACGATCTCCTCGAGTCAGCCATTAAAACCTACCAACAATACTTCCATCTTAGCCCCACTGGATTCCTGAATGCCGAGACGATATCCCAACTCGCAACACCTCGGTGCGGCGTTCCAGATATCGTCAATGGAACTACCGATCGAATGCTATCAGAACACAACGACAACGACGGTGATCATGATCACCATCACCACCACCTTCCTCACGTCGTCTCGCACTATGCCTTCTTCCCAGGAAGGCGCAGGTGGCCATCTACCAAATACCATCTCACCTACGCGTTTCTTCCCGGCACTCGAGCAGACGCGAAAGCACCGGTGGCTCGAGCGTTCGCGACGTGGGCTCGAAACACTCATTTTAAGTTTTCTTTGGCTACAAACTATAAAAGAGCTGACTTGAAGATTGGTTTCTATAGAGGAAACCATGGAGATGGATACCCATTTGATGGGCCAGGTGGGACTTTGGCACATGCATTTGCTCCAACTGATGGGAGATTTCATTATGATTCTACAGAGAAATGGGGAGTTGGGGCAGTGAGAGGGCGATATGATTTGCAAACGGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGACATAGCACTGTTAAGAATGCTATAATGTATCCTTATATCAATTCAGGGACTACAAAGGGTTTGAATGTTGATGATATCAAAGGAATCAAGGTTTTATACAATAGACACTAG

Protein sequence

MANKPLVLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGLNVDDIKGIKVLYNRH
BLAST of Lsi06G006840 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.1e-58
Identity = 122/293 (41.64%), Postives = 174/293 (59.39%), Query Frame = 1

Query: 29  FEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIK 88
           ++  ++  GC  G NV G+ ++K +F  +GY+             +  D FDD+L++A++
Sbjct: 41  WDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPET-------FSGNFTDDFDDILKAAVE 100

Query: 89  TYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPH 148
            YQ  F+L+ TG L+A TI  +  PRCG PD+VNGT+  ++        + +    HL H
Sbjct: 101 LYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTS--LMHGGRRKTFEVNFSRTHL-H 160

Query: 149 VVSHYAFFPGRRRWPSTKYHLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATNY 208
            V  Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   F+L+ ++
Sbjct: 161 AVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESF 220

Query: 209 KRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVG-------AVRG 268
             +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V  
Sbjct: 221 STSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTA 280

Query: 269 RYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
             DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 281 AVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of Lsi06G006840 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 9.1e-58
Identity = 134/329 (40.73%), Postives = 182/329 (55.32%), Query Frame = 1

Query: 7   VLLFISIFLPLC--FSLPLLQVSPFEFLNDLQ----------GCKKGDNVKGISKLKNFF 66
           +L+F     P+   F   +  + P +FLN  Q          GC  G+N+ G+SKLK +F
Sbjct: 8   ILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYF 67

Query: 67  CYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPR 126
             +GY+    N T         D FDD+L+SAI TYQ+ F+L  TG L++ T+ Q+  PR
Sbjct: 68  RRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPR 127

Query: 127 CGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFL 186
           CG PD+++G ++        N G        +      Y+FFPG+ RWP  K  LTYAF 
Sbjct: 128 CGNPDLIDGVSEM-------NGGK-------ILRTTEKYSFFPGKPRWPKRKRDLTYAFA 187

Query: 187 PGTRA--DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGT 246
           P      + K   +RAF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG  GT
Sbjct: 188 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 247

Query: 247 LAHAFAPTDGRFHYDSTEKWGV--GAVRGR-------YDLQTVALHEIGHLLGLGHSTVK 306
           LAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+V+
Sbjct: 248 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 307

Query: 307 NAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           +AIM+P I+ G  K  L  DDI+GI+ LY
Sbjct: 308 DAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of Lsi06G006840 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 123/296 (41.55%), Postives = 168/296 (56.76%), Query Frame = 1

Query: 27  SPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESA 86
           +P+    +  GC  G    G+  LK +F ++GY+   TN +G+       D FDD+L++A
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYITE-TNLSGNFT-----DDFDDILKNA 100

Query: 87  IKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHL 146
           ++ YQ+ F L+ TG L+  T+  +  PRCG PD+VNGT+  M S     +          
Sbjct: 101 VEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTS-TMHSGRKTFEVSFAGRGQRF 160

Query: 147 PHVVSHYAFFPGRRRWPSTKYHLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLAT 206
            H V HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   F+   
Sbjct: 161 -HAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVE 220

Query: 207 NYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKW--------GVGA 266
            +  +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W        G  +
Sbjct: 221 RFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFIS 280

Query: 267 VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           V    DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++G++ LY
Sbjct: 281 VSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of Lsi06G006840 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 220.3 bits (560), Expect = 2.9e-56
Identity = 124/280 (44.29%), Postives = 158/280 (56.43%), Query Frame = 1

Query: 41  GDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTG 100
           G N KG+S +KN+F + GY+ +  +          DD FDD L SAIKTYQ+ ++L+ TG
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPH---------FDDNFDDTLVSAIKTYQKNYNLNVTG 107

Query: 101 FLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRR 160
             +  T+ Q+ TPRCGVPDI+  T                        ++S Y FF    
Sbjct: 108 KFDINTLKQIMTPRCGVPDIIINTNKTTSFG-----------------MISDYTFFKDMP 167

Query: 161 RWPSTKYHLTYAFLPGTRADA--KAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRG 220
           RW +    LTYAF P  R D   K+ +ARAF+ W    +  F   T+Y+ A++KI F   
Sbjct: 168 RWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASK 227

Query: 221 NHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGA------VRGRYDLQTVALHEIG 280
           NHGD YPFDGPGG L HAFAPTDGR H+D+ E W          V   +DL++VA+HEIG
Sbjct: 228 NHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIG 287

Query: 281 HLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           HLLGLGHS+   AIMYP I   T K  L  DDI GI+ LY
Sbjct: 288 HLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

BLAST of Lsi06G006840 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.3e-47
Identity = 113/283 (39.93%), Postives = 152/283 (53.71%), Query Frame = 1

Query: 41  GDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTG 100
           G +V G+S+LK +   +GY+N  +            D FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEI--------FSDVFDGPLESAISLYQENLGLPITG 123

Query: 101 FLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRR 160
            L+  T++ ++ PRCGV       +D  ++ +ND             H  +HY +F G+ 
Sbjct: 124 RLDTSTVTLMSLPRCGV-------SDTHMTINND-----------FLHTTAHYTYFNGKP 183

Query: 161 RWPSTKYHLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIG 220
           +W   +  LTYA          T  D K    RAF+ W+      F    ++  ADLKIG
Sbjct: 184 KW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIG 243

Query: 221 FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGV-----GAVRGRYDLQTVALH 280
           FY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA H
Sbjct: 244 FYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATH 303

Query: 281 EIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           EIGHLLGLGHS+ ++A+MYP +   T K  L VDD+ G+  LY
Sbjct: 304 EIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of Lsi06G006840 vs. TrEMBL
Match: A0A0A0M0J6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 2.9e-159
Identity = 276/315 (87.62%), Postives = 287/315 (91.11%), Query Frame = 1

Query: 1   MANKPL--VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYG 60
           M NKPL  +LL ISIFLPLCFSLPL+QVSPF FLNDLQGCKKGDNVKGISKLKNFF YYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVP 120
           YLNH+ NATGHLI+ D +D FDD LESAIKTYQQYFHL+PTG LNAET+SQLATPRCG P
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTR 180
           DI+N TT RMLSE  DN   HDHHH  LPH VSHYAFFPGR RWPSTKY LTYAFLPGTR
Sbjct: 121 DIINETTGRMLSEDIDNVSSHDHHH--LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPVARAFATWARNTHFKF+L TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKG 300
           PTDGRFHYDSTEKW VGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI SG+TKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKG 300

Query: 301 LNVDDIKGIKVLYNR 314
           LNVDDIKGIKVLYNR
Sbjct: 301 LNVDDIKGIKVLYNR 313

BLAST of Lsi06G006840 vs. TrEMBL
Match: A0A0A0M032_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 6.7e-140
Identity = 248/313 (79.23%), Postives = 268/313 (85.62%), Query Frame = 1

Query: 1   MANKPL-VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           M +KPL +LLFISI LPLCFSLPL QVS   FLNDLQG KKGDNVKGISKLKNFF  YGY
Sbjct: 1   MGSKPLAILLFISI-LPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ N TGHLI+ D DDTFDD  ESA+KTYQQYFHL+ TG LNAET+SQLATPRCG PD
Sbjct: 61  LNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I+N  T RML E+N+ND  HDH+H  L H V HY+FFPGR RWP TKYHLTY FLP T A
Sbjct: 121 ILNEATGRMLLENNNNDSSHDHYHQ-LSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWAR+THFKFSLATN +RADLKIGFYRGNHGDGYPFDG GGTLAHAF P
Sbjct: 181 DAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGR H+DSTEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I SG+TKGL
Sbjct: 241 TDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGL 300

Query: 301 NVDDIKGIKVLYN 313
           N DDI+GI+VLYN
Sbjct: 301 NADDIEGIEVLYN 311

BLAST of Lsi06G006840 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.5e-94
Identity = 177/321 (55.14%), Postives = 223/321 (69.47%), Query Frame = 1

Query: 1   MANKPLVLLFISIFL--PLCFSLPLL-------QVSPFEFLNDLQGCKKGDNVKGISKLK 60
           MA+KP  +  +++ L   L  S  L        + S F+FL  LQGC KGDN+KGI  LK
Sbjct: 1   MASKPFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLK 60

Query: 61  NFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLA 120
            +   +GYL+++  +  +      DD FDDLLE A+KTYQ  +HL+ TGFL++ET++++ 
Sbjct: 61  KYLENFGYLSYKNQSHSN------DDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMM 120

Query: 121 TPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTY 180
            PRCGV DI+NGTT    S  N +     HH     H VSHY FFPG  RWP++KYHLTY
Sbjct: 121 MPRCGVADIINGTTRMQSSNKNPH-----HHSSTSFHTVSHYEFFPGNPRWPASKYHLTY 180

Query: 181 AFLPGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGG 240
            FLPGT   A  PVA+AF TWA NTHF+F+   +Y+ AD+ IGF+RG+HGDG PFDG GG
Sbjct: 181 GFLPGTPNQAMEPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGG 240

Query: 241 TLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI 300
           TLAHAFAP DGRFHYD  E W VGA +G +D++TVALHEIGHLLGLGHS+V+ AIM+P I
Sbjct: 241 TLAHAFAPQDGRFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSI 300

Query: 301 NSGTTKGLNVDDIKGIKVLYN 313
            SG TKGL+ DDI+GI+ LYN
Sbjct: 301 QSGATKGLHSDDIQGIRALYN 310

BLAST of Lsi06G006840 vs. TrEMBL
Match: B9RUG7_RICCO (Matrix metalloproteinase, putative OS=Ricinus communis GN=RCOM_0852700 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.4e-91
Identity = 175/322 (54.35%), Postives = 224/322 (69.57%), Query Frame = 1

Query: 1   MANKPLVLLFISIFLPL------CFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFF 60
           MA+KP + + +S  +          ++   +  PFEFL  LQGC KGD +KG+ +LK + 
Sbjct: 1   MASKPFLPILLSTLIAFTSLVSHAAAVADTKSLPFEFLKHLQGCHKGDKLKGVGELKKYL 60

Query: 61  CYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPR 120
            ++GYL++  N + H  E D DD FDD LESAI+TYQ  +HL+ +G L++ET S++  PR
Sbjct: 61  KHFGYLSYNKNQS-HANEDD-DDDFDDPLESAIRTYQLNYHLNVSGLLDSETASKMMMPR 120

Query: 121 CGVPDIVNGTTDRMLSEHNDNDGDHDHHHH-HLP---HVVSHYAFFPGRRRWPSTKYHLT 180
           CGV DI+NGTT  M S    +   H HHH  H P   H VSHY+FF G  +WP +KYHLT
Sbjct: 121 CGVADIINGTT-WMESGRKHHRHPHRHHHRRHGPKSLHKVSHYSFFSGNPKWPPSKYHLT 180

Query: 181 YAFLPGTRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPG 240
           Y+FLPG    A  PVA AF TWA NTHF FS   +Y  +D+ I FYRG+HGDG+PFDGPG
Sbjct: 181 YSFLPGFPTAAVNPVANAFETWAANTHFSFSWTRDYINSDILISFYRGDHGDGHPFDGPG 240

Query: 241 GTLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPY 300
           GTLAHAFAP +G FHYD+ E+W +GAV G YDL+T ALHEIGHLLGLGHS+V+ AIMYP 
Sbjct: 241 GTLAHAFAPQNGLFHYDADERWSIGAVLGAYDLETTALHEIGHLLGLGHSSVEGAIMYPQ 300

Query: 301 INSGTTKGLNVDDIKGIKVLYN 313
           I +G T+GL+ DD++GI+ LYN
Sbjct: 301 IFAGETRGLHSDDLEGIRTLYN 319

BLAST of Lsi06G006840 vs. TrEMBL
Match: S1SMU3_THECC (Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.5e-91
Identity = 181/306 (59.15%), Postives = 209/306 (68.30%), Query Frame = 1

Query: 13  IFLPLCFSLPLLQVS-----PFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNAT 72
           + LPL F   L         PF+FL  LQGC KGD VK I KLK +   +GYL++  N T
Sbjct: 15  LVLPLLFQATLADSKDKKPYPFDFLKHLQGCHKGDKVKDIRKLKKYLEQFGYLSYSKNKT 74

Query: 73  GHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDR 132
                A+ DD FDDLLESAIKTYQ  FHL+  G L+ ET+S++  PRCGV DI+NGT+  
Sbjct: 75  ----HAN-DDDFDDLLESAIKTYQLNFHLNSNGALDTETVSKMMMPRCGVADIINGTSGM 134

Query: 133 MLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRADAKAPVAR 192
              +       H        H VSHYAFFP   RWP +K HLTYAFLPGTRADA  PVA 
Sbjct: 135 RSGKKKP----HRAAGSKSIHEVSHYAFFPRSPRWPPSKSHLTYAFLPGTRADAVNPVAG 194

Query: 193 AFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYD 252
           AF TWA NTHF+FS   NY+ AD+ IGF R +HGDG PFDGPGGTLAHAFAPT GRFHYD
Sbjct: 195 AFQTWAANTHFRFSRIDNYRDADITIGFQRRDHGDGNPFDGPGGTLAHAFAPTLGRFHYD 254

Query: 253 STEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGLNVDDIKGI 312
           + E W V A  G   L+TVALHEIGHLLGLGHS+++NAIMYP I +GT+KGL  DDI+GI
Sbjct: 255 ADETWSVSARPGTMHLETVALHEIGHLLGLGHSSIENAIMYPSITAGTSKGLARDDIEGI 311

Query: 313 KVLYNR 314
           K LYNR
Sbjct: 315 KALYNR 311

BLAST of Lsi06G006840 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 226.9 bits (577), Expect = 1.8e-59
Identity = 122/293 (41.64%), Postives = 174/293 (59.39%), Query Frame = 1

Query: 29  FEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIK 88
           ++  ++  GC  G NV G+ ++K +F  +GY+             +  D FDD+L++A++
Sbjct: 41  WDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPET-------FSGNFTDDFDDILKAAVE 100

Query: 89  TYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPH 148
            YQ  F+L+ TG L+A TI  +  PRCG PD+VNGT+  ++        + +    HL H
Sbjct: 101 LYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTS--LMHGGRRKTFEVNFSRTHL-H 160

Query: 149 VVSHYAFFPGRRRWPSTKYHLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATNY 208
            V  Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   F+L+ ++
Sbjct: 161 AVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESF 220

Query: 209 KRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVG-------AVRG 268
             +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V  
Sbjct: 221 STSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTA 280

Query: 269 RYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
             DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 281 AVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of Lsi06G006840 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 225.3 bits (573), Expect = 5.2e-59
Identity = 134/329 (40.73%), Postives = 182/329 (55.32%), Query Frame = 1

Query: 7   VLLFISIFLPLC--FSLPLLQVSPFEFLNDLQ----------GCKKGDNVKGISKLKNFF 66
           +L+F     P+   F   +  + P +FLN  Q          GC  G+N+ G+SKLK +F
Sbjct: 8   ILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYF 67

Query: 67  CYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPR 126
             +GY+    N T         D FDD+L+SAI TYQ+ F+L  TG L++ T+ Q+  PR
Sbjct: 68  RRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPR 127

Query: 127 CGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFL 186
           CG PD+++G ++        N G        +      Y+FFPG+ RWP  K  LTYAF 
Sbjct: 128 CGNPDLIDGVSEM-------NGGK-------ILRTTEKYSFFPGKPRWPKRKRDLTYAFA 187

Query: 187 PGTRA--DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGT 246
           P      + K   +RAF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG  GT
Sbjct: 188 PQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGT 247

Query: 247 LAHAFAPTDGRFHYDSTEKWGV--GAVRGR-------YDLQTVALHEIGHLLGLGHSTVK 306
           LAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+V+
Sbjct: 248 LAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVE 307

Query: 307 NAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           +AIM+P I+ G  K  L  DDI+GI+ LY
Sbjct: 308 DAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of Lsi06G006840 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 224.9 bits (572), Expect = 6.7e-59
Identity = 123/296 (41.55%), Postives = 168/296 (56.76%), Query Frame = 1

Query: 27  SPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESA 86
           +P+    +  GC  G    G+  LK +F ++GY+   TN +G+       D FDD+L++A
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYITE-TNLSGNFT-----DDFDDILKNA 100

Query: 87  IKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHL 146
           ++ YQ+ F L+ TG L+  T+  +  PRCG PD+VNGT+  M S     +          
Sbjct: 101 VEMYQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTS-TMHSGRKTFEVSFAGRGQRF 160

Query: 147 PHVVSHYAFFPGRRRWPSTKYHLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLAT 206
            H V HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   F+   
Sbjct: 161 -HAVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVE 220

Query: 207 NYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKW--------GVGA 266
            +  +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W        G  +
Sbjct: 221 RFSTSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFIS 280

Query: 267 VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           V    DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++G++ LY
Sbjct: 281 VSEAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of Lsi06G006840 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 189.1 bits (479), Expect = 4.1e-48
Identity = 113/283 (39.93%), Postives = 152/283 (53.71%), Query Frame = 1

Query: 41  GDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTG 100
           G +V G+S+LK +   +GY+N  +            D FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEI--------FSDVFDGPLESAISLYQENLGLPITG 123

Query: 101 FLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRR 160
            L+  T++ ++ PRCGV       +D  ++ +ND             H  +HY +F G+ 
Sbjct: 124 RLDTSTVTLMSLPRCGV-------SDTHMTINND-----------FLHTTAHYTYFNGKP 183

Query: 161 RWPSTKYHLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATNYKRADLKIG 220
           +W   +  LTYA          T  D K    RAF+ W+      F    ++  ADLKIG
Sbjct: 184 KW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIG 243

Query: 221 FYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGV-----GAVRGRYDLQTVALH 280
           FY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA H
Sbjct: 244 FYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATH 303

Query: 281 EIGHLLGLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           EIGHLLGLGHS+ ++A+MYP +   T K  L VDD+ G+  LY
Sbjct: 304 EIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of Lsi06G006840 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 166.4 bits (420), Expect = 2.8e-41
Identity = 106/277 (38.27%), Postives = 136/277 (49.10%), Query Frame = 1

Query: 47  ISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAET 106
           I ++K     YGYL           E+D     D   E A+  YQ+   L  TG  +++T
Sbjct: 50  IPEIKRHLQQYGYLPQNK-------ESD-----DVSFEQALVRYQKNLGLPITGKPDSDT 109

Query: 107 ISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWP-ST 166
           +SQ+  PRCG PD V   T                      H    Y +FPGR RW    
Sbjct: 110 LSQILLPRCGFPDDVEPKTAPF-------------------HTGKKYVYFPGRPRWTRDV 169

Query: 167 KYHLTYAFLPGTRADAKAPV------ARAFATWARNTHFKFSLATNYKRADLKIGFYRGN 226
              LTYAF         AP        RAF  WA      F    +Y  AD+KIGF+ G+
Sbjct: 170 PLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGD 229

Query: 227 HGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGR----YDLQTVALHEIGHLL 286
           HGDG PFDG  G LAH F+P +GR H D  E W V     +     DL++VA+HEIGH+L
Sbjct: 230 HGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVL 289

Query: 287 GLGHSTVKNAIMYPYINSGTTK-GLNVDDIKGIKVLY 312
           GLGHS+VK+A MYP +   + K  LN+DD+ G++ LY
Sbjct: 290 GLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

BLAST of Lsi06G006840 vs. NCBI nr
Match: gi|659085906|ref|XP_008443669.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 578.2 bits (1489), Expect = 8.9e-162
Identity = 277/314 (88.22%), Postives = 289/314 (92.04%), Query Frame = 1

Query: 1   MANKPL-VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           M NK L +LLFIS+FLPLCFSLPLLQVSPF FLNDLQGCKKGDNVKGISKLKNFF YYGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ NATGHLI+ D DDTFDD LE AIKTYQQYFHL+PTG LNAETISQLATPRCG PD
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I+N +T RMLSEHN+ND  HDHHH  LPH VSHYAFFPGRRRWPSTKY LTYAFLPGTRA
Sbjct: 121 IINESTGRMLSEHNNNDSSHDHHH--LPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRA 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWARNTHFKFSL TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP
Sbjct: 181 DAKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGRFHYDSTEKW VGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYI SG+TKGL
Sbjct: 241 TDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGL 300

Query: 301 NVDDIKGIKVLYNR 314
           N DDIKGIKVLYNR
Sbjct: 301 NADDIKGIKVLYNR 312

BLAST of Lsi06G006840 vs. NCBI nr
Match: gi|449442791|ref|XP_004139164.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 569.3 bits (1466), Expect = 4.1e-159
Identity = 276/315 (87.62%), Postives = 287/315 (91.11%), Query Frame = 1

Query: 1   MANKPL--VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYG 60
           M NKPL  +LL ISIFLPLCFSLPL+QVSPF FLNDLQGCKKGDNVKGISKLKNFF YYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVP 120
           YLNH+ NATGHLI+ D +D FDD LESAIKTYQQYFHL+PTG LNAET+SQLATPRCG P
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTR 180
           DI+N TT RMLSE  DN   HDHHH  LPH VSHYAFFPGR RWPSTKY LTYAFLPGTR
Sbjct: 121 DIINETTGRMLSEDIDNVSSHDHHH--LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPVARAFATWARNTHFKF+L TNY+RADLKIGFYRGNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKG 300
           PTDGRFHYDSTEKW VGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYI SG+TKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKG 300

Query: 301 LNVDDIKGIKVLYNR 314
           LNVDDIKGIKVLYNR
Sbjct: 301 LNVDDIKGIKVLYNR 313

BLAST of Lsi06G006840 vs. NCBI nr
Match: gi|449442789|ref|XP_004139163.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 505.0 bits (1299), Expect = 9.6e-140
Identity = 248/313 (79.23%), Postives = 268/313 (85.62%), Query Frame = 1

Query: 1   MANKPL-VLLFISIFLPLCFSLPLLQVSPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGY 60
           M +KPL +LLFISI LPLCFSLPL QVS   FLNDLQG KKGDNVKGISKLKNFF  YGY
Sbjct: 1   MGSKPLAILLFISI-LPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGY 60

Query: 61  LNHRTNATGHLIEADVDDTFDDLLESAIKTYQQYFHLSPTGFLNAETISQLATPRCGVPD 120
           LNH+ N TGHLI+ D DDTFDD  ESA+KTYQQYFHL+ TG LNAET+SQLATPRCG PD
Sbjct: 61  LNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNPD 120

Query: 121 IVNGTTDRMLSEHNDNDGDHDHHHHHLPHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRA 180
           I+N  T RML E+N+ND  HDH+H  L H V HY+FFPGR RWP TKYHLTY FLP T A
Sbjct: 121 ILNEATGRMLLENNNNDSSHDHYHQ-LSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA 180

Query: 181 DAKAPVARAFATWARNTHFKFSLATNYKRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWAR+THFKFSLATN +RADLKIGFYRGNHGDGYPFDG GGTLAHAF P
Sbjct: 181 DAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTP 240

Query: 241 TDGRFHYDSTEKWGVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGL 300
           TDGR H+DSTEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I SG+TKGL
Sbjct: 241 TDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGL 300

Query: 301 NVDDIKGIKVLYN 313
           N DDI+GI+VLYN
Sbjct: 301 NADDIEGIEVLYN 311

BLAST of Lsi06G006840 vs. NCBI nr
Match: gi|645249271|ref|XP_008230675.1| (PREDICTED: metalloendoproteinase 1-like [Prunus mume])

HSP 1 Score: 357.1 bits (915), Expect = 3.2e-95
Identity = 176/286 (61.54%), Postives = 207/286 (72.38%), Query Frame = 1

Query: 27  SPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESA 86
           SPFEFL  L+GC KGD V+GI  LK +   +GYL+   N  GH      DD FDD LESA
Sbjct: 32  SPFEFLEHLKGCHKGDKVQGIQDLKKYLGKFGYLSSNNN--GHFN----DDDFDDQLESA 91

Query: 87  IKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHL 146
           IKTYQ  +HL  TG L+AET+S +  PRCGV DI+NGT+     +       H HHHHH 
Sbjct: 92  IKTYQLNYHLKATGTLDAETVSNMMMPRCGVADIINGTSSMRSGKQR-----HPHHHHHG 151

Query: 147 PHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNY 206
            H V+HY FF G  +WP++KYHLTYAFL GT A+A  PVARAF TWA NTHF FS +   
Sbjct: 152 GHTVAHYTFFRGNPKWPASKYHLTYAFLQGTPAEATGPVARAFQTWAANTHFTFSQSN-- 211

Query: 207 KRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTV 266
           +  DL + F+RGNHGDG PFDGPGGT+AHAFAPT+GRFHYD+ E++ VGAV G YDL+TV
Sbjct: 212 QNPDLTVSFHRGNHGDGSPFDGPGGTIAHAFAPTNGRFHYDADERFSVGAVSGAYDLETV 271

Query: 267 ALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGLNVDDIKGIKVLYN 313
           ALHEIGHLLGLGHS+V  AIMYP I+ G TKGL+ DDI+GIK LYN
Sbjct: 272 ALHEIGHLLGLGHSSVLGAIMYPTISPGATKGLHGDDIQGIKALYN 304

BLAST of Lsi06G006840 vs. NCBI nr
Match: gi|645249909|ref|XP_008230960.1| (PREDICTED: metalloendoproteinase 1-like [Prunus mume])

HSP 1 Score: 357.1 bits (915), Expect = 3.2e-95
Identity = 176/286 (61.54%), Postives = 207/286 (72.38%), Query Frame = 1

Query: 27  SPFEFLNDLQGCKKGDNVKGISKLKNFFCYYGYLNHRTNATGHLIEADVDDTFDDLLESA 86
           SPFEFL  L+GC KGD V+GI  LK +   +GYL+   N  GH      DD FDD LESA
Sbjct: 40  SPFEFLEHLKGCHKGDKVQGIQDLKKYLGKFGYLSSNNN--GHFN----DDDFDDQLESA 99

Query: 87  IKTYQQYFHLSPTGFLNAETISQLATPRCGVPDIVNGTTDRMLSEHNDNDGDHDHHHHHL 146
           IKTYQ  +HL  TG L+AET+S +  PRCGV DI+NGT+     +       H HHHHH 
Sbjct: 100 IKTYQLNYHLKATGTLDAETVSNMMMPRCGVADIINGTSSMRSGKQR-----HPHHHHHG 159

Query: 147 PHVVSHYAFFPGRRRWPSTKYHLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNY 206
            H V+HY FF G  +WP++KYHLTYAFL GT A+A  PVARAF TWA NTHF FS +   
Sbjct: 160 GHTVAHYTFFRGNPKWPASKYHLTYAFLQGTPAEATGPVARAFQTWAANTHFTFSQSN-- 219

Query: 207 KRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWGVGAVRGRYDLQTV 266
           +  DL + F+RGNHGDG PFDGPGGT+AHAFAPT+GRFHYD+ E++ VGAV G YDL+TV
Sbjct: 220 QNPDLTVSFHRGNHGDGSPFDGPGGTIAHAFAPTNGRFHYDADERFSVGAVSGAYDLETV 279

Query: 267 ALHEIGHLLGLGHSTVKNAIMYPYINSGTTKGLNVDDIKGIKVLYN 313
           ALHEIGHLLGLGHS+V  AIMYP I+ G TKGL+ DDI+GIK LYN
Sbjct: 280 ALHEIGHLLGLGHSSVLGAIMYPTISPGATKGLHGDDIQGIKALYN 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
2MMP_ARATH3.1e-5841.64Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH9.1e-5840.73Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
3MMP_ARATH1.2e-5741.55Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
MEP1_SOYBN2.9e-5644.29Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
1MMP_ARATH7.3e-4739.93Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M0J6_CUCSA2.9e-15987.62Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1[more]
A0A0A0M032_CUCSA6.7e-14079.23Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1[more]
B9RUG6_RICCO5.5e-9455.14Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1[more]
B9RUG7_RICCO3.4e-9154.35Matrix metalloproteinase, putative OS=Ricinus communis GN=RCOM_0852700 PE=3 SV=1[more]
S1SMU3_THECC7.5e-9159.15Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G70170.11.8e-5941.64 matrix metalloproteinase[more]
AT1G59970.15.2e-5940.73 Matrixin family protein[more]
AT1G24140.16.7e-5941.55 Matrixin family protein[more]
AT4G16640.14.1e-4839.93 Matrixin family protein[more]
AT2G45040.12.8e-4138.27 Matrixin family protein[more]
Match NameE-valueIdentityDescription
gi|659085906|ref|XP_008443669.1|8.9e-16288.22PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|449442791|ref|XP_004139164.1|4.1e-15987.62PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|449442789|ref|XP_004139163.1|9.6e-14079.23PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|645249271|ref|XP_008230675.1|3.2e-9561.54PREDICTED: metalloendoproteinase 1-like [Prunus mume][more]
gi|645249909|ref|XP_008230960.1|3.2e-9561.54PREDICTED: metalloendoproteinase 1-like [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0004222metalloendopeptidase activity
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR024079MetalloPept_cat_dom_sf
IPR021190Pept_M10A
IPR021158Pept_M10A_Zn_BS
IPR006026Peptidase_Metallo
IPR002477Peptidoglycan-bd-like
IPR001818Pept_M10_metallopeptidase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0051252 regulation of RNA metabolic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0006396 RNA processing
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004386 helicase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0008237 metallopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G006840.1Lsi06G006840.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 162..311
score: 3.0
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 47..112
score: 5.2
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 57..110
score: 5.
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 39..120
score: 1.1
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 158..313
score: 2.8
IPR021158Peptidase M10A, cysteine switch, zinc binding sitePROSITEPS00546CYSTEINE_SWITCHcoord: 113..120
scor
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 299..312
score: 2.2E-36coord: 209..237
score: 2.2E-36coord: 110..123
score: 2.2E-36coord: 185..200
score: 2.2E-36coord: 266..291
score: 2.2
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 113..312
score: 3.0
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 1..123
score: 1.5E-122coord: 148..311
score: 1.5E
NoneNo IPR availablePANTHERPTHR10201:SF140F20P5.11 PROTEINcoord: 148..311
score: 1.5E-122coord: 1..123
score: 1.5E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 155..312
score: 1.54