Bhi02G001100 (gene) Wax gourd

NameBhi02G001100
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionMatrix metalloproteinase, putative
Locationchr2 : 32625079 .. 32626029 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAATAAACCTTTGGTAATTCTTCTCTTCATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCGTTGCTCCAAGTTTCTCCATTTGCATTTCTCAATGACCTCCAAGGATGTAAGAAAGGTGATCATGTCAATGGCATATCCAAGCTTAAGAACTTTTTCCATTACTATGGTTATTTAAACCATCGAATCAACGTCACTGGCCATTTGATCGAAGCTGACTTCGACGATACCTTTGACGATTTCCTCGAGTCAGCCATCAAAACCTACCAACAATATTTCCATCTCAACCCCACTGGACTCCTTGATGTTGAGACGATATCCCAACTCGCAACACCTCGATGCGGTGTTCCAGATATCATCAATGGAACTACCAGTCGAATGTTATCAGAACATGACAACGATAACGACAGTGAACATGATCACCATCACCATCACCTTCCTCACGCCGTCTCGCACTACGCTTTCTTCCCAGGAAGGCGTAGGTGGCCATCAACCAAATACCGCCTCACATATGCATTTCTTCCTGGCACTCGTGCGGATGCAAAAGCACCGGTGGCTCGAGCATTCGCGACGTGGGCTCGGAACACCCATTTTAAGTTTTCTTTGGCCACAAACTATAGAAGAGCTGACTTGAAGATAGGTTTCTATGGAGGAAACCATGGAGATGGGTACCCATTTGATGGGCCAGGGGGGACTTTGGCACATGCTTTTGCTCCAACTGATGGAAGATTTCATTATGATTCAACGGAGAAATGGGCTGTTGGGGCAGTGAAAGGGAGATATGATTTACAAACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCAATGTTAGGAATGCTATAATGTATCCTTATATCAAATCAGGGACTACTAAGGGTTTGAATGCAGATGATATCAAAGGAATCAAGGTTTTATACAACAGACGCTAA

mRNA sequence

ATGGCGAATAAACCTTTGGTAATTCTTCTCTTCATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCGTTGCTCCAAGTTTCTCCATTTGCATTTCTCAATGACCTCCAAGGATGTAAGAAAGGTGATCATGTCAATGGCATATCCAAGCTTAAGAACTTTTTCCATTACTATGGTTATTTAAACCATCGAATCAACGTCACTGGCCATTTGATCGAAGCTGACTTCGACGATACCTTTGACGATTTCCTCGAGTCAGCCATCAAAACCTACCAACAATATTTCCATCTCAACCCCACTGGACTCCTTGATGTTGAGACGATATCCCAACTCGCAACACCTCGATGCGGTGTTCCAGATATCATCAATGGAACTACCAGTCGAATGTTATCAGAACATGACAACGATAACGACAGTGAACATGATCACCATCACCATCACCTTCCTCACGCCGTCTCGCACTACGCTTTCTTCCCAGGAAGGCGTAGGTGGCCATCAACCAAATACCGCCTCACATATGCATTTCTTCCTGGCACTCGTGCGGATGCAAAAGCACCGGTGGCTCGAGCATTCGCGACGTGGGCTCGGAACACCCATTTTAAGTTTTCTTTGGCCACAAACTATAGAAGAGCTGACTTGAAGATAGGTTTCTATGGAGGAAACCATGGAGATGGGTACCCATTTGATGGGCCAGGGGGGACTTTGGCACATGCTTTTGCTCCAACTGATGGAAGATTTCATTATGATTCAACGGAGAAATGGGCTGTTGGGGCAGTGAAAGGGAGATATGATTTACAAACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCAATGTTAGGAATGCTATAATGTATCCTTATATCAAATCAGGGACTACTAAGGGTTTGAATGCAGATGATATCAAAGGAATCAAGGTTTTATACAACAGACGCTAA

Coding sequence (CDS)

ATGGCGAATAAACCTTTGGTAATTCTTCTCTTCATCTCCATCTTCCTTCCTCTTTGCTTTTCACTTCCGTTGCTCCAAGTTTCTCCATTTGCATTTCTCAATGACCTCCAAGGATGTAAGAAAGGTGATCATGTCAATGGCATATCCAAGCTTAAGAACTTTTTCCATTACTATGGTTATTTAAACCATCGAATCAACGTCACTGGCCATTTGATCGAAGCTGACTTCGACGATACCTTTGACGATTTCCTCGAGTCAGCCATCAAAACCTACCAACAATATTTCCATCTCAACCCCACTGGACTCCTTGATGTTGAGACGATATCCCAACTCGCAACACCTCGATGCGGTGTTCCAGATATCATCAATGGAACTACCAGTCGAATGTTATCAGAACATGACAACGATAACGACAGTGAACATGATCACCATCACCATCACCTTCCTCACGCCGTCTCGCACTACGCTTTCTTCCCAGGAAGGCGTAGGTGGCCATCAACCAAATACCGCCTCACATATGCATTTCTTCCTGGCACTCGTGCGGATGCAAAAGCACCGGTGGCTCGAGCATTCGCGACGTGGGCTCGGAACACCCATTTTAAGTTTTCTTTGGCCACAAACTATAGAAGAGCTGACTTGAAGATAGGTTTCTATGGAGGAAACCATGGAGATGGGTACCCATTTGATGGGCCAGGGGGGACTTTGGCACATGCTTTTGCTCCAACTGATGGAAGATTTCATTATGATTCAACGGAGAAATGGGCTGTTGGGGCAGTGAAAGGGAGATATGATTTACAAACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCAATGTTAGGAATGCTATAATGTATCCTTATATCAAATCAGGGACTACTAAGGGTTTGAATGCAGATGATATCAAAGGAATCAAGGTTTTATACAACAGACGCTAA

Protein sequence

MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPDIINGTTSRMLSEHDNDNDSEHDHHHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKGLNADDIKGIKVLYNRR
BLAST of Bhi02G001100 vs. Swiss-Prot
Match: sp|Q5XF51|3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.1e-61
Identity = 125/294 (42.52%), Postives = 169/294 (57.48%), Query Frame = 0

Query: 31  AFLNDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKT 90
           +FLN   GC  G   +G+  LK +F ++GY+    N++G     +F D FDD L++A++ 
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSG-----NFTDDFDDILKNAVEM 104

Query: 91  YQQYFHLNPTGLLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPH 150
           YQ+ F LN TG+LD  T+  +  PRCG PD++NGT++                      H
Sbjct: 105 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRF---H 164

Query: 151 AVSHYAFFPGRRRWPSTKYRLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLATNY 210
           AV HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   F+    +
Sbjct: 165 AVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERF 224

Query: 211 RRADLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AVK 270
             +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V 
Sbjct: 225 STSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVS 284

Query: 271 GRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
              DL++VA+HEIGHLLGLGHS+V  +IMYP I++G  K  L  DD++G++ LY
Sbjct: 285 EAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of Bhi02G001100 vs. Swiss-Prot
Match: sp|O04529|2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 5.9e-60
Identity = 122/290 (42.07%), Postives = 168/290 (57.93%), Query Frame = 0

Query: 34  NDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQ 93
           ++  GC  G +V+G+ ++K +F  +GY+    +        +F D FDD L++A++ YQ 
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFS-------GNFTDDFDDILKAAVELYQT 104

Query: 94  YFHLNPTGLLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVS 153
            F+LN TG LD  TI  +  PRCG PD++NGT+      H                HAV 
Sbjct: 105 NFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLM----HGGRRKTFEVNFSRTHLHAVK 164

Query: 154 HYAFFPGRRRWPSTKYRLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATNYRRA 213
            Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   F+L+ ++  +
Sbjct: 165 RYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTS 224

Query: 214 DLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVKGRYD 273
           D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V    D
Sbjct: 225 DITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVD 284

Query: 274 LQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           L++VA+HEIGHLLGLGHS+V  +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 285 LESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of Bhi02G001100 vs. Swiss-Prot
Match: sp|P29136|MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 8.5e-59
Identity = 130/284 (45.77%), Postives = 162/284 (57.04%), Query Frame = 0

Query: 42  GDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTG 101
           G +  G+S +KN+FH+ GY+ +            FDD FDD L SAIKTYQ+ ++LN TG
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPN---------APHFDDNFDDTLVSAIKTYQKNYNLNVTG 107

Query: 102 LLDVETISQLATPRCGVPDII---NGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFF 161
             D+ T+ Q+ TPRCGVPDII   N TTS  +                     +S Y FF
Sbjct: 108 KFDINTLKQIMTPRCGVPDIIINTNKTTSFGM---------------------ISDYTFF 167

Query: 162 PGRRRWPSTKYRLTYAFLPGTRAD--AKAPVARAFATWARNTHFKFSLATNYRRADLKIG 221
               RW +   +LTYAF P  R D   K+ +ARAF+ W    +  F   T+Y  A++KI 
Sbjct: 168 KDMPRWQAGTTQLTYAFSPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKIL 227

Query: 222 FYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGA------VKGRYDLQTVAL 281
           F   NHGD YPFDGPGG L HAFAPTDGR H+D+ E W          V   +DL++VA+
Sbjct: 228 FASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAV 287

Query: 282 HEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           HEIGHLLGLGHS+   AIMYP I   T K  L  DDI GI+ LY
Sbjct: 288 HEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRKLY 301

BLAST of Bhi02G001100 vs. Swiss-Prot
Match: sp|Q9ZUJ5|5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.7e-57
Identity = 136/332 (40.96%), Postives = 176/332 (53.01%), Query Frame = 0

Query: 6   LVILLFISIFLPLC--FSLPLLQVSPFAFLN----------DLQGCKKGDHVNGISKLKN 65
           L IL+F     P+   F   +  + P  FLN           L GC  G+++NG+SKLK 
Sbjct: 6   LTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQ 65

Query: 66  FFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLAT 125
           +F  +GY+    N T         D FDD L+SAI TYQ+ F+L  TG LD  T+ Q+  
Sbjct: 66  YFRRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVK 125

Query: 126 PRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTY 185
           PRCG PD+I+G +                     +      Y+FFPG+ RWP  K  LTY
Sbjct: 126 PRCGNPDLIDGVSE---------------MNGGKILRTTEKYSFFPGKPRWPKRKRDLTY 185

Query: 186 AFLPGTRA--DAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGP 245
           AF P      + K   +RAF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG 
Sbjct: 186 AFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGA 245

Query: 246 GGTLAHAFAPTDGRFHYDSTEKWAV--GAVKGR-------YDLQTVALHEIGHLLGLGHS 305
            GTLAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS
Sbjct: 246 MGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHS 305

Query: 306 NVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           +V +AIM+P I  G  K  L  DDI+GI+ LY
Sbjct: 306 SVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of Bhi02G001100 vs. Swiss-Prot
Match: sp|O23507|1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 7.2e-50
Identity = 115/284 (40.49%), Postives = 151/284 (53.17%), Query Frame = 0

Query: 42  GDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTG 101
           G HV+G+S+LK + H +GY+N    +        F D FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEI--------FSDVFDGPLESAISLYQENLGLPITG 123

Query: 102 LLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGR 161
            LD  T++ ++ PRCGV D      +  L                   H  +HY +F G+
Sbjct: 124 RLDTSTVTLMSLPRCGVSDTHMTINNDFL-------------------HTTAHYTYFNGK 183

Query: 162 RRWPSTKYRLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATNYRRADLKI 221
            +W   +  LTYA          T  D K    RAF+ W+      F    ++  ADLKI
Sbjct: 184 PKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKI 243

Query: 222 GFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GAVKGRYDLQTVAL 281
           GFY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+ +   DL++VA 
Sbjct: 244 GFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVAT 303

Query: 282 HEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           HEIGHLLGLGHS+  +A+MYP ++  T K  L  DD+ G+  LY
Sbjct: 304 HEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of Bhi02G001100 vs. TAIR10
Match: AT1G24140.1 (Matrixin family protein)

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-62
Identity = 125/294 (42.52%), Postives = 169/294 (57.48%), Query Frame = 0

Query: 31  AFLNDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKT 90
           +FLN   GC  G   +G+  LK +F ++GY+    N++G     +F D FDD L++A++ 
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSG-----NFTDDFDDILKNAVEM 104

Query: 91  YQQYFHLNPTGLLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPH 150
           YQ+ F LN TG+LD  T+  +  PRCG PD++NGT++                      H
Sbjct: 105 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRF---H 164

Query: 151 AVSHYAFFPGRRRWPSTKYRLTYAFLP--GTRADAKAPVARAFATWARNTHFKFSLATNY 210
           AV HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   F+    +
Sbjct: 165 AVKHYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERF 224

Query: 211 RRADLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AVK 270
             +D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V 
Sbjct: 225 STSDISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVS 284

Query: 271 GRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
              DL++VA+HEIGHLLGLGHS+V  +IMYP I++G  K  L  DD++G++ LY
Sbjct: 285 EAVDLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of Bhi02G001100 vs. TAIR10
Match: AT1G70170.1 (matrix metalloproteinase)

HSP 1 Score: 232.6 bits (592), Expect = 3.2e-61
Identity = 122/290 (42.07%), Postives = 168/290 (57.93%), Query Frame = 0

Query: 34  NDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQ 93
           ++  GC  G +V+G+ ++K +F  +GY+    +        +F D FDD L++A++ YQ 
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFS-------GNFTDDFDDILKAAVELYQT 104

Query: 94  YFHLNPTGLLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVS 153
            F+LN TG LD  TI  +  PRCG PD++NGT+      H                HAV 
Sbjct: 105 NFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLM----HGGRRKTFEVNFSRTHLHAVK 164

Query: 154 HYAFFPGRRRWPSTKYRLTYAFLPGT--RADAKAPVARAFATWARNTHFKFSLATNYRRA 213
            Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   F+L+ ++  +
Sbjct: 165 RYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTS 224

Query: 214 DLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVKGRYD 273
           D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V    D
Sbjct: 225 DITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVD 284

Query: 274 LQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           L++VA+HEIGHLLGLGHS+V  +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 285 LESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of Bhi02G001100 vs. TAIR10
Match: AT1G59970.1 (Matrixin family protein)

HSP 1 Score: 223.8 bits (569), Expect = 1.5e-58
Identity = 136/332 (40.96%), Postives = 176/332 (53.01%), Query Frame = 0

Query: 6   LVILLFISIFLPLC--FSLPLLQVSPFAFLN----------DLQGCKKGDHVNGISKLKN 65
           L IL+F     P+   F   +  + P  FLN           L GC  G+++NG+SKLK 
Sbjct: 6   LTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQ 65

Query: 66  FFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLAT 125
           +F  +GY+    N T         D FDD L+SAI TYQ+ F+L  TG LD  T+ Q+  
Sbjct: 66  YFRRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVK 125

Query: 126 PRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTY 185
           PRCG PD+I+G +                     +      Y+FFPG+ RWP  K  LTY
Sbjct: 126 PRCGNPDLIDGVSE---------------MNGGKILRTTEKYSFFPGKPRWPKRKRDLTY 185

Query: 186 AFLPGTRA--DAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGP 245
           AF P      + K   +RAF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG 
Sbjct: 186 AFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGA 245

Query: 246 GGTLAHAFAPTDGRFHYDSTEKWAV--GAVKGR-------YDLQTVALHEIGHLLGLGHS 305
            GTLAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS
Sbjct: 246 MGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHS 305

Query: 306 NVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           +V +AIM+P I  G  K  L  DDI+GI+ LY
Sbjct: 306 SVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of Bhi02G001100 vs. TAIR10
Match: AT4G16640.1 (Matrixin family protein)

HSP 1 Score: 199.1 bits (505), Expect = 4.0e-51
Identity = 115/284 (40.49%), Postives = 151/284 (53.17%), Query Frame = 0

Query: 42  GDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTG 101
           G HV+G+S+LK + H +GY+N    +        F D FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEI--------FSDVFDGPLESAISLYQENLGLPITG 123

Query: 102 LLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGR 161
            LD  T++ ++ PRCGV D      +  L                   H  +HY +F G+
Sbjct: 124 RLDTSTVTLMSLPRCGVSDTHMTINNDFL-------------------HTTAHYTYFNGK 183

Query: 162 RRWPSTKYRLTYAFLPG------TRADAKAPVARAFATWARNTHFKFSLATNYRRADLKI 221
            +W   +  LTYA          T  D K    RAF+ W+      F    ++  ADLKI
Sbjct: 184 PKW--NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKI 243

Query: 222 GFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GAVKGRYDLQTVAL 281
           GFY G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+ +   DL++VA 
Sbjct: 244 GFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVAT 303

Query: 282 HEIGHLLGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           HEIGHLLGLGHS+  +A+MYP ++  T K  L  DD+ G+  LY
Sbjct: 304 HEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of Bhi02G001100 vs. TAIR10
Match: AT2G45040.1 (Matrixin family protein)

HSP 1 Score: 169.9 bits (429), Expect = 2.6e-42
Identity = 105/278 (37.77%), Postives = 138/278 (49.64%), Query Frame = 0

Query: 48  ISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVET 107
           I ++K     YGYL                ++ D   E A+  YQ+   L  TG  D +T
Sbjct: 50  IPEIKRHLQQYGYLPQN------------KESDDVSFEQALVRYQKNLGLPITGKPDSDT 109

Query: 108 ISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWP-S 167
           +SQ+  PRCG PD +   T+                      H    Y +FPGR RW   
Sbjct: 110 LSQILLPRCGFPDDVEPKTAPF--------------------HTGKKYVYFPGRPRWTRD 169

Query: 168 TKYRLTYAFLPGTRADAKAPV------ARAFATWARNTHFKFSLATNYRRADLKIGFYGG 227
              +LTYAF         AP        RAF  WA      F    +Y  AD+KIGF+ G
Sbjct: 170 VPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNG 229

Query: 228 NHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGR----YDLQTVALHEIGHL 287
           +HGDG PFDG  G LAH F+P +GR H D  E WAV   + +     DL++VA+HEIGH+
Sbjct: 230 DHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHV 289

Query: 288 LGLGHSNVRNAIMYPYIKSGTTK-GLNADDIKGIKVLY 314
           LGLGHS+V++A MYP +K  + K  LN DD+ G++ LY
Sbjct: 290 LGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

BLAST of Bhi02G001100 vs. TrEMBL
Match: tr|A0A1S3B8J8|A0A1S3B8J8_CUCME (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103487215 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 2.2e-157
Identity = 285/316 (90.19%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGY 60
           M NK L ILLFIS+FLPLCFSLPLLQVSPFAFLNDLQGCKKGD+V GISKLKNFF YYGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPD 120
           LNH+IN TGHLI+ D DDTFDD LE AIKTYQQYFHLNPTG L+ ETISQLATPRCG PD
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 IINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180
           IIN +T RMLSEH    XXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR
Sbjct: 121 IINESTGRMLSEH---NXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPV RAFATWARNTHFKFSL TNYRRADLKIGFY GNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKG 300
           PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHS VRNAIMYPYI+SG+TKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKG 300

Query: 301 LNADDIKGIKVLYNRR 317
           LNADDIKGIKVLYNRR
Sbjct: 301 LNADDIKGIKVLYNRR 313

BLAST of Bhi02G001100 vs. TrEMBL
Match: tr|A0A0A0M0J6|A0A0A0M0J6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 2.6e-153
Identity = 269/317 (84.86%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 1   MANKPL-VILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYG 60
           M NKPL ++LL ISIFLPLCFSLPL+QVSPFAFLNDLQGCKKGD+V GISKLKNFF YYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVP 120
           YLNH+IN TGHLI+ D +D FDD LESAIKTYQQYFHLNPTG L+ ET+SQLATPRCG P
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGT 180
           DIIN TT RMLSE               LPHAVSHYAFFPGR RWPSTKYRLTYAFLPGT
Sbjct: 121 DIINETTGRMLSE---DIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGT 180

Query: 181 RADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAF 240
           RADAKAPVARAFATWARNTHFKF+L TNYRRADLKIGFY GNHGDGYPFDGPGGTLAHAF
Sbjct: 181 RADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAF 240

Query: 241 APTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTK 300
           APTDGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHS V+NAIMYPYIKSG+TK
Sbjct: 241 APTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK 300

Query: 301 GLNADDIKGIKVLYNRR 317
           GLN DDIKGIKVLYNRR
Sbjct: 301 GLNVDDIKGIKVLYNRR 314

BLAST of Bhi02G001100 vs. TrEMBL
Match: tr|A0A1S4DUX9|A0A1S4DUX9_CUCME (LOW QUALITY PROTEIN: metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC103487472 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.8e-139
Identity = 246/315 (78.10%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGY 60
           MANKPLVILLFISIFLPLCFS PLL VSPFAFLN LQGC KGD+V GIS LKNFFH+YGY
Sbjct: 1   MANKPLVILLFISIFLPLCFSTPLLHVSPFAFLNKLQGCTKGDNVKGISNLKNFFHHYGY 60

Query: 61  LNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPD 120
           LNH+IN T HLI+ + DDTFDD LESAIKTYQQYFHLN TG L+VETISQLA PRCG PD
Sbjct: 61  LNHQINATSHLIDINVDDTFDDRLESAIKTYQQYFHLNSTGSLNVETISQLAKPRCGNPD 120

Query: 121 IINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180
           II+ T  RML EH              LPH VSH+ FF G RRWPSTKY LTYAFLPGTR
Sbjct: 121 IIDETADRMLLEH----NSDSRHDYHHLPHIVSHFTFFSGMRRWPSTKYHLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFA 240
            D KAPVARAFATWAR THF FSLA NYR+A+LKIGFY GNHGDGY FDGPG TLAH+FA
Sbjct: 181 VDVKAPVARAFATWARYTHFXFSLAKNYRKANLKIGFYRGNHGDGYSFDGPGRTLAHSFA 240

Query: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKG 300
           PTDGRFHYDS EKW VGAV+GRYD+QTVALHEIGHLLGLGHS +RN+IMYPYIKSG TKG
Sbjct: 241 PTDGRFHYDSAEKWTVGAVRGRYDMQTVALHEIGHLLGLGHSKIRNSIMYPYIKSGATKG 300

Query: 301 LNADDIKGIKVLYNR 316
           L+ DDI GIKVLY+R
Sbjct: 301 LSLDDILGIKVLYHR 311

BLAST of Bhi02G001100 vs. TrEMBL
Match: tr|A0A0A0M032|A0A0A0M032_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G654840 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.3e-133
Identity = 240/314 (76.43%), Postives = 261/314 (83.12%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGY 60
           M +KPL ILLFISI LPLCFSLPL QVS  +FLNDLQG KKGD+V GISKLKNFF  YGY
Sbjct: 1   MGSKPLAILLFISI-LPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGY 60

Query: 61  LNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPD 120
           LNH+INVTGHLI+ D DDTFDD  ESA+KTYQQYFHLN TG L+ ET+SQLATPRCG PD
Sbjct: 61  LNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNPD 120

Query: 121 IINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180
           I+N  T RML E+              L HAV HY+FFPGR RWP TKY LTY FLP T 
Sbjct: 121 ILNEATGRMLLEN--NNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTH 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPV RAFATWAR+THFKFSLATN RRADLKIGFY GNHGDGYPFDG GGTLAHAF 
Sbjct: 181 ADAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFT 240

Query: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKG 300
           PTDGR H+DSTEKW VGAV+GR+DL+TVALHEIGHLLGLGHS V+NAIMYP I+SG+TKG
Sbjct: 241 PTDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKG 300

Query: 301 LNADDIKGIKVLYN 315
           LNADDI+GI+VLYN
Sbjct: 301 LNADDIEGIEVLYN 311

BLAST of Bhi02G001100 vs. TrEMBL
Match: tr|A0A2I4FIH9|A0A2I4FIH9_9ROSI (metalloendoproteinase 2-MMP-like OS=Juglans regia OX=51240 GN=LOC108999108 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.4e-90
Identity = 171/289 (59.17%), Postives = 198/289 (68.51%), Query Frame = 0

Query: 26  QVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLE 85
           + SPF FL  LQGC KG+++ GI  LK +   +GY  H+I    H      DD FD+ LE
Sbjct: 37  KTSPFGFLKHLQGCHKGENLKGIHDLKIYLENFGYY-HKIENNTHA----NDDDFDELLE 96

Query: 86  SAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXX 145
           SAIKTYQQ +HL  TG LD ET+S++  PRCGV DIINGT S    +             
Sbjct: 97  SAIKTYQQNYHLKATGTLDAETVSKMMMPRCGVADIINGTNSMQSGKKKHHHLGSF---- 156

Query: 146 XXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLA 205
               H VSHY+FF G  RWP +KYRLTY FLPGT   A  PVARAF TWARNTHF FSLA
Sbjct: 157 ----HIVSHYSFFQGAPRWPPSKYRLTYRFLPGTPTQAMNPVARAFQTWARNTHFTFSLA 216

Query: 206 TNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDL 265
                AD+ IGF   +HGDG PFDGPGGT+AHAFAPTDGRFHYD+ E+++VGA  G +D 
Sbjct: 217 QAPANADITIGFLRRDHGDGAPFDGPGGTIAHAFAPTDGRFHYDADERYSVGATPGAFDW 276

Query: 266 QTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKGLNADDIKGIKVLYN 315
           +TVALHEIGHLLGLGHS+VR AIM P+I  G TKGLNADDI GI+ LYN
Sbjct: 277 ETVALHEIGHLLGLGHSSVRGAIMEPFISQGVTKGLNADDIAGIRALYN 312

BLAST of Bhi02G001100 vs. NCBI nr
Match: XP_008443669.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 563.9 bits (1452), Expect = 3.4e-157
Identity = 285/316 (90.19%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGY 60
           M NK L ILLFIS+FLPLCFSLPLLQVSPFAFLNDLQGCKKGD+V GISKLKNFF YYGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPD 120
           LNH+IN TGHLI+ D DDTFDD LE AIKTYQQYFHLNPTG L+ ETISQLATPRCG PD
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 IINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180
           IIN +T RMLSEH    XXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR
Sbjct: 121 IINESTGRMLSEH---NXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFA 240
           ADAKAPV RAFATWARNTHFKFSL TNYRRADLKIGFY GNHGDGYPFDGPGGTLAHAFA
Sbjct: 181 ADAKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 240

Query: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKG 300
           PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHS VRNAIMYPYI+SG+TKG
Sbjct: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKG 300

Query: 301 LNADDIKGIKVLYNRR 317
           LNADDIKGIKVLYNRR
Sbjct: 301 LNADDIKGIKVLYNRR 313

BLAST of Bhi02G001100 vs. NCBI nr
Match: XP_004139164.1 (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus] >KGN66657.1 hypothetical protein Csa_1G654860 [Cucumis sativus])

HSP 1 Score: 550.4 bits (1417), Expect = 3.9e-153
Identity = 269/317 (84.86%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 1   MANKPL-VILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYG 60
           M NKPL ++LL ISIFLPLCFSLPL+QVSPFAFLNDLQGCKKGD+V GISKLKNFF YYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVP 120
           YLNH+IN TGHLI+ D +D FDD LESAIKTYQQYFHLNPTG L+ ET+SQLATPRCG P
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGT 180
           DIIN TT RMLSE               LPHAVSHYAFFPGR RWPSTKYRLTYAFLPGT
Sbjct: 121 DIINETTGRMLSE---DIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGT 180

Query: 181 RADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAF 240
           RADAKAPVARAFATWARNTHFKF+L TNYRRADLKIGFY GNHGDGYPFDGPGGTLAHAF
Sbjct: 181 RADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAF 240

Query: 241 APTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTK 300
           APTDGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHS V+NAIMYPYIKSG+TK
Sbjct: 241 APTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK 300

Query: 301 GLNADDIKGIKVLYNRR 317
           GLN DDIKGIKVLYNRR
Sbjct: 301 GLNVDDIKGIKVLYNRR 314

BLAST of Bhi02G001100 vs. NCBI nr
Match: XP_022961167.1 (metalloendoproteinase 1-like [Cucurbita moschata])

HSP 1 Score: 520.0 bits (1338), Expect = 5.6e-144
Identity = 259/328 (78.96%), Postives = 274/328 (83.54%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPL------------LQVSPFAFLNDLQGCKKGDHVNGI 60
           MANKPL++  F  IFLPLC SLPL                PFAFLNDLQGCKKGD+V GI
Sbjct: 1   MANKPLILAFF--IFLPLCLSLPLPXXXXXXXXXXXXXXXPFAFLNDLQGCKKGDNVKGI 60

Query: 61  SKLKNFFHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETI 120
           SKLKNFFHYYGYLNH INVTG+L   D DD FDD LESAIKTYQ+YFHLNPTG L++ETI
Sbjct: 61  SKLKNFFHYYGYLNHGINVTGYL--NDVDDVFDDPLESAIKTYQRYFHLNPTGTLNLETI 120

Query: 121 SQLATPRCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTK 180
           SQLATPRCGVPDI+NGTT R+L EH              L H VSHYAFFPG+RRWPS+K
Sbjct: 121 SQLATPRCGVPDIVNGTTGRLLFEH----DDDGHVHRYHLSHVVSHYAFFPGKRRWPSSK 180

Query: 181 YRLTYAFLPGTRADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPF 240
           YRLTYAFLPGTR DAKAPV RAFATWAR THFKFSL TNY+RA+LKIGFY GNHGDGYPF
Sbjct: 181 YRLTYAFLPGTRKDAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPF 240

Query: 241 DGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAI 300
           DGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHS V+NAI
Sbjct: 241 DGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAI 300

Query: 301 MYPYIKSGTTKGLNADDIKGIKVLYNRR 317
           MYPYIKSGTTKGLNADDIKGIKVLYN+R
Sbjct: 301 MYPYIKSGTTKGLNADDIKGIKVLYNKR 320

BLAST of Bhi02G001100 vs. NCBI nr
Match: XP_023516970.1 (metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.8 bits (1322), Expect = 4.0e-142
Identity = 270/322 (83.85%), Postives = 285/322 (88.51%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPLLQ------VSPFAFLNDLQGCKKGDHVNGISKLKNF 60
           MANKPL++  F  IFLPLC SLPL +      VSPFAFLNDLQGCKKGD+V GISKLKNF
Sbjct: 1   MANKPLILAFF--IFLPLCLSLPLPELPELPRVSPFAFLNDLQGCKKGDNVKGISKLKNF 60

Query: 61  FHYYGYLNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATP 120
           FHYYGYLN R NVT ++   D +D FDD LE AIKTYQQYFHLNPTG L+ ETISQLATP
Sbjct: 61  FHYYGYLNRRTNVTSYM--NDDNDVFDDHLEFAIKTYQQYFHLNPTGTLNPETISQLATP 120

Query: 121 RCGVPDIINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYA 180
           RCGVPDI+NGTT R+L   XXXXXXXXXXXXXXLPH VSHYAFFPG+RRWPS+KYRLTYA
Sbjct: 121 RCGVPDIVNGTTGRLLF-XXXXXXXXXXXXXXXLPHVVSHYAFFPGKRRWPSSKYRLTYA 180

Query: 181 FLPGTRADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGT 240
           FLP TR DAKAPV RAFATWAR THFKFSL TNY+RA+LKIGFY GNHGDGYPFDGPGGT
Sbjct: 181 FLPSTRKDAKAPVTRAFATWARYTHFKFSLTTNYKRANLKIGFYKGNHGDGYPFDGPGGT 240

Query: 241 LAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIK 300
           LAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHS V+NAIMYPYIK
Sbjct: 241 LAHAFAPTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIK 300

Query: 301 SGTTKGLNADDIKGIKVLYNRR 317
           SGTTKGLNADDIKGIKVLYNRR
Sbjct: 301 SGTTKGLNADDIKGIKVLYNRR 317

BLAST of Bhi02G001100 vs. NCBI nr
Match: XP_016899789.1 (PREDICTED: LOW QUALITY PROTEIN: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 503.8 bits (1296), Expect = 4.2e-139
Identity = 246/315 (78.10%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MANKPLVILLFISIFLPLCFSLPLLQVSPFAFLNDLQGCKKGDHVNGISKLKNFFHYYGY 60
           MANKPLVILLFISIFLPLCFS PLL VSPFAFLN LQGC KGD+V GIS LKNFFH+YGY
Sbjct: 1   MANKPLVILLFISIFLPLCFSTPLLHVSPFAFLNKLQGCTKGDNVKGISNLKNFFHHYGY 60

Query: 61  LNHRINVTGHLIEADFDDTFDDFLESAIKTYQQYFHLNPTGLLDVETISQLATPRCGVPD 120
           LNH+IN T HLI+ + DDTFDD LESAIKTYQQYFHLN TG L+VETISQLA PRCG PD
Sbjct: 61  LNHQINATSHLIDINVDDTFDDRLESAIKTYQQYFHLNSTGSLNVETISQLAKPRCGNPD 120

Query: 121 IINGTTSRMLSEHXXXXXXXXXXXXXXLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTR 180
           II+ T  RML EH              LPH VSH+ FF G RRWPSTKY LTYAFLPGTR
Sbjct: 121 IIDETADRMLLEH----NSDSRHDYHHLPHIVSHFTFFSGMRRWPSTKYHLTYAFLPGTR 180

Query: 181 ADAKAPVARAFATWARNTHFKFSLATNYRRADLKIGFYGGNHGDGYPFDGPGGTLAHAFA 240
            D KAPVARAFATWAR THF FSLA NYR+A+LKIGFY GNHGDGY FDGPG TLAH+FA
Sbjct: 181 VDVKAPVARAFATWARYTHFXFSLAKNYRKANLKIGFYRGNHGDGYSFDGPGRTLAHSFA 240

Query: 241 PTDGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSNVRNAIMYPYIKSGTTKG 300
           PTDGRFHYDS EKW VGAV+GRYD+QTVALHEIGHLLGLGHS +RN+IMYPYIKSG TKG
Sbjct: 241 PTDGRFHYDSAEKWTVGAVRGRYDMQTVALHEIGHLLGLGHSKIRNSIMYPYIKSGATKG 300

Query: 301 LNADDIKGIKVLYNR 316
           L+ DDI GIKVLY+R
Sbjct: 301 LSLDDILGIKVLYHR 311

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q5XF51|3MMP_ARATH3.1e-6142.52Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
sp|O04529|2MMP_ARATH5.9e-6042.07Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
sp|P29136|MEP1_SOYBN8.5e-5945.77Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
sp|Q9ZUJ5|5MMP_ARATH2.7e-5740.96Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
sp|O23507|1MMP_ARATH7.2e-5040.49Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24140.11.7e-6242.52Matrixin family protein[more]
AT1G70170.13.2e-6142.07matrix metalloproteinase[more]
AT1G59970.11.5e-5840.96Matrixin family protein[more]
AT4G16640.14.0e-5140.49Matrixin family protein[more]
AT2G45040.12.6e-4237.77Matrixin family protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3B8J8|A0A1S3B8J8_CUCME2.2e-15790.19metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103487215 PE=3 SV... [more]
tr|A0A0A0M0J6|A0A0A0M0J6_CUCSA2.6e-15384.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G654860 PE=3 SV=1[more]
tr|A0A1S4DUX9|A0A1S4DUX9_CUCME2.8e-13978.10LOW QUALITY PROTEIN: metalloendoproteinase 1-like OS=Cucumis melo OX=3656 GN=LOC... [more]
tr|A0A0A0M032|A0A0A0M032_CUCSA1.3e-13376.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G654840 PE=3 SV=1[more]
tr|A0A2I4FIH9|A0A2I4FIH9_9ROSI2.4e-9059.17metalloendoproteinase 2-MMP-like OS=Juglans regia OX=51240 GN=LOC108999108 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_008443669.13.4e-15790.19PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo][more]
XP_004139164.13.9e-15384.86PREDICTED: metalloendoproteinase 1-like [Cucumis sativus] >KGN66657.1 hypothetic... [more]
XP_022961167.15.6e-14478.96metalloendoproteinase 1-like [Cucurbita moschata][more]
XP_023516970.14.0e-14283.85metalloendoproteinase 1-like [Cucurbita pepo subsp. pepo][more]
XP_016899789.14.2e-13978.10PREDICTED: LOW QUALITY PROTEIN: metalloendoproteinase 1-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0008237metallopeptidase activity
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR036365PGBD-like_sf
IPR033739M10A_MMP
IPR021158Pept_M10A_Zn_BS
IPR002477Peptidoglycan-bd-like
IPR001818Pept_M10_metallopeptidase
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi02M001100Bhi02M001100mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 111..124
score: 50.52
coord: 187..202
score: 33.23
coord: 211..239
score: 62.28
coord: 301..314
score: 51.57
coord: 268..293
score: 58.3
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 160..315
e-value: 8.3E-34
score: 128.3
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 168..313
e-value: 2.8E-45
score: 153.9
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 48..111
e-value: 2.8E-9
score: 37.0
NoneNo IPR availablePANTHERPTHR10201:SF213METALLOENDOPROTEINASE 2-MMP-RELATEDcoord: 23..314
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 23..314
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 157..314
IPR021158Peptidase M10A, cysteine switch, zinc binding sitePROSITEPS00546CYSTEINE_SWITCHcoord: 114..121
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 163..313
e-value: 5.43791E-60
score: 190.493
IPR036365PGBD-like superfamilySUPERFAMILYSSF47090PGBD-likecoord: 40..121

The following gene(s) are paralogous to this gene:

None