Cucsa.356580 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.356580
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionMatrix metalloproteinase, putative
Locationscaffold03577 : 1392227 .. 1393174 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAGTAAACCTCTGGCAATTCTTCTCTTCATCTCCATCCTCCCTCTTTGCTTTTCACTTCCATTGCGCCAAGTTTCTCGATTATCATTTCTCAATGACCTCCAAGGAAGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAGCTTAAGAACTTTTTCCGTCGTTACGGTTATTTAAATCATCAAATCAATGTCACTGGTCATTTGATCGACCATGATGCCGACGACACCTTTGACGATCGCTTCGAGTCTGCCGTCAAAACCTACCAACAATACTTCCATCTAAACTCCACTGGATCTCTGAATGCCGAGACGCTATCCCAACTTGCAACACCTCGGTGCGGTAATCCAGATATCCTTAATGAAGCCACTGGTCGAATGCTATTAGAAAaCAACAACAACGATAGTAGTCACGATCACTACCACCAACTCTCCCATGCTGTACCACACTACAGTTTCTTCCCAGGAAGGCCTAGGTGGCCACCTACCAAATATCACTTAACATATGAGTTTCTTCCCAACACTCATGCAGACGCAAAAGCACCGGTGACACGAGCGTTCGCAACATGGGCTCGACACACACATTTTAAGTTTtCTTTGGCCACAAACTCTAGAAGAGCTGATTTGAAGATAGGGTTTTATAGAGGGAACCATGGAGATGGGTACCCATTTGACGGGTCAGGAGGAACTTTGGCACATGCTTTTACTCCAACTGATGGGAGAGTTCATTTTGATTCAACAGAGAAATGGGTAGTTGGGGCAGTAAGAGGGAGATTTGATTTGGAAACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCAGAGTTAAAAATGCTATAATGTATCCTACTATAGAATCTGGGTCTACTAAAGGCTTGAATGCTGATGACATTGAAGGGATTGAGGTTTTATACAACGTACCTTTACCCTAA

mRNA sequence

ATGGGGAGTAAACCTCTGGCAATTCTTCTCTTCATCTCCATCCTCCCTCTTTGCTTTTCACTTCCATTGCGCCAAGTTTCTCGATTATCATTTCTCAATGACCTCCAAGGAAGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAGCTTAAGAACTTTTTCCGTCGTTACGGTTATTTAAATCATCAAATCAATGTCACTGGTCATTTGATCGACCATGATGCCGACGACACCTTTGACGATCGCTTCGAGTCTGCCGTCAAAACCTACCAACAATACTTCCATCTAAACTCCACTGGATCTCTGAATGCCGAGACGCTATCCCAACTTGCAACACCTCGTCACGATCACTACCACCAACTCTCCCATGCTGTACCACACTACAGTTTCTTCCCAGGAAGGCCTAGGTGGCCACCTACCAAATATCACTTAACATATGAGTTTCTTCCCAACACTCATGCAGACGCAAAAGCACCGGTGACACGAGCGTTCGCAACATGGGCTCGACACACACATTTTAAGTTTTCTTTGGCCACAAACTCTAGAAGAGCTGATTTGAAGATAGGGTTTTATAGAGGGAACCATGGAGATGGGTACCCATTTGACGGGTCAGGAGGAACTTTGGCACATGCTTTTACTCCAACTGATGGGAGAGTTCATTTTGATTCAACAGAGAAATGGGTAGTTGGGGCAGTAAGAGGGAGATTTGATTTGGAAACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCAGAGTTAAAAATGCTATAATGTATCCTACTATAGAATCTGGGTCTACTAAAGGCTTGAATGCTGATGACATTGAAGGGATTGAGGTTTTATACAACGTACCTTTACCCTAA

Coding sequence (CDS)

ATGGGGAGTAAACCTCTGGCAATTCTTCTCTTCATCTCCATCCTCCCTCTTTGCTTTTCACTTCCATTGCGCCAAGTTTCTCGATTATCATTTCTCAATGACCTCCAAGGAAGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAGCTTAAGAACTTTTTCCGTCGTTACGGTTATTTAAATCATCAAATCAATGTCACTGGTCATTTGATCGACCATGATGCCGACGACACCTTTGACGATCGCTTCGAGTCTGCCGTCAAAACCTACCAACAATACTTCCATCTAAACTCCACTGGATCTCTGAATGCCGAGACGCTATCCCAACTTGCAACACCTCGTCACGATCACTACCACCAACTCTCCCATGCTGTACCACACTACAGTTTCTTCCCAGGAAGGCCTAGGTGGCCACCTACCAAATATCACTTAACATATGAGTTTCTTCCCAACACTCATGCAGACGCAAAAGCACCGGTGACACGAGCGTTCGCAACATGGGCTCGACACACACATTTTAAGTTTtCTTTGGCCACAAACTCTAGAAGAGCTGATTTGAAGATAGGGTTTTATAGAGGGAACCATGGAGATGGGTACCCATTTGACGGGTCAGGAGGAACTTTGGCACATGCTTTTACTCCAACTGATGGGAGAGTTCATTTTGATTCAACAGAGAAATGGGTAGTTGGGGCAGTAAGAGGGAGATTTGATTTGGAAACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCAGAGTTAAAAATGCTATAATGTATCCTACTATAGAATCTGGGTCTACTAAAGGCTTGAATGCTGATGACATTGAAGGGATTGAGGTTTTATACAACGTACCTTTACCCTAA

Protein sequence

MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNADDIEGIEVLYNVPLP*
Homology
[Close]
BLAST of Cucsa.356580 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.1e-56
Identity = 130/314 (41.40%), Postives = 173/314 (55.10%), Query Frame = 1 

Query: 8   ILLFISILPLC--FSLPLRQVSRLSFLNDLQ----------GSKKGDNVKGISKLKNFFR 67
           ++ F ++ P+   F   +  +  L FLN  Q          G   G+N+ G+SKLK +FR
Sbjct: 9   LIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYFR 68

Query: 68  RYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRH 127
           R+GY+    N T         D FDD  +SA+ TYQ+ F+L  TG L++ TL Q+  PR 
Sbjct: 69  RFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRC 128

Query: 128 DHYH-----------QLSHAVPHYSFFPGRPRWPPTKYHLTYEFLP--NTHADAKAPVTR 187
            +             ++      YSFFPG+PRWP  K  LTY F P  N   + K   +R
Sbjct: 129 GNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSR 188

Query: 188 AFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFD 247
           AF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG+ GTLAHA +P  G +H D
Sbjct: 189 AFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLD 248

Query: 248 STEKWVV--GAVRGRF-------DLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTK- 287
             E W++  G +  R        DLE+VA+HEIGHLLGLGHS V++AIM+P I  G  K 
Sbjct: 249 GDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKV 308

[Close]
BLAST of Cucsa.356580 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.8e-53
Identity = 119/298 (39.93%), Postives = 166/298 (55.70%), Query Frame = 1 

Query: 23  LRQVSRLSF--LNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFD 82
           LR  +R+ +   ++  G   G NV G+ ++K +F+R+GY+    +        +  D FD
Sbjct: 33  LRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFS-------GNFTDDFD 92

Query: 83  DRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRHDHYHQLS------------------ 142
           D  ++AV+ YQ  F+LN TG L+A T+  +  PR  +   ++                  
Sbjct: 93  DILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFS 152

Query: 143 ----HAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA--DAKAPVTRAFATWARHTHFKFS 202
               HAV  Y+ FPG PRWP  +  LTY F P      + K+  +RAF  W+  T   F+
Sbjct: 153 RTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFT 212

Query: 203 LATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKWVVG------ 262
           L+ +   +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+ H D+ E WVV       
Sbjct: 213 LSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSF 272

Query: 263 -AVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTK-GLNADDIEGIEVLY 287
            +V    DLE+VA+HEIGHLLGLGHS V+ +IMYPTI +G  K  L  DD+EGI+ LY
Sbjct: 273 LSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of Cucsa.356580 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 9.1e-52
Identity = 120/291 (41.24%), Postives = 154/291 (52.92%), Query Frame = 1 

Query: 30  SFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKT 89
           SFLN   G   G    G+  LK +F+ +GY+  + N++G+  D      FDD  ++AV+ 
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSGNFTDD-----FDDILKNAVEM 104

Query: 90  YQQYFHLNSTGSLNAETLSQLATPRHDHYH-----------------------QLSHAVP 149
           YQ+ F LN TG L+  TL  +  PR  +                         Q  HAV 
Sbjct: 105 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK 164

Query: 150 HYSFFPGRPRWPPTKYHLTYEFLPNTHA--DAKAPVTRAFATWARHTHFKFSLATNSRRA 209
           HYSFFPG PRWP  +  LTY F P      + K+  +RAF  W   T   F+       +
Sbjct: 165 HYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 224

Query: 210 DLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKWVVG--------AVRGRF 269
           D+ IGFY G HGDG PFDG   TLAHAF+P  G  H D  E W+V         +V    
Sbjct: 225 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 284

Query: 270 DLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTK-GLNADDIEGIEVLY 287
           DLE+VA+HEIGHLLGLGHS V+ +IMYPTI +G  K  L  DD+EG++ LY
Sbjct: 285 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of Cucsa.356580 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.0e-50
Identity = 114/265 (43.02%), Postives = 144/265 (54.34%), Query Frame = 1 

Query: 41  GDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTG 100
           G N KG+S +KN+F   GY+ +  +          DD FDD   SA+KTYQ+ ++LN TG
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHF---------DDNFDDTLVSAIKTYQKNYNLNVTG 107

Query: 101 SLNAETLSQLATPR--------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNT 160
             +  TL Q+ TPR        + +       +  Y+FF   PRW      LTY F P  
Sbjct: 108 KFDINTLKQIMTPRCGVPDIIINTNKTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEP 167

Query: 161 HADA--KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAH 220
             D   K+ + RAF+ W    +  F   T+   A++KI F   NHGD YPFDG GG L H
Sbjct: 168 RLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGH 227

Query: 221 AFTPTDGRVHFDSTEKWVVGA------VRGRFDLETVALHEIGHLLGLGHSRVKNAIMYP 280
           AF PTDGR HFD+ E WV         V   FDLE+VA+HEIGHLLGLGHS    AIMYP
Sbjct: 228 AFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYP 287

Query: 281 TIESGSTK-GLNADDIEGIEVLYNV 289
           +I   + K  L  DDI+GI  LY +
Sbjct: 288 SIPPRTRKVNLAQDDIDGIRKLYGI 303

[Close]
BLAST of Cucsa.356580 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.2e-45
Identity = 105/265 (39.62%), Postives = 145/265 (54.72%), Query Frame = 1 

Query: 41  GDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTG 100
           G +V G+S+LK +  R+GY+N    +          D FD   ESA+  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS--------DVFDGPLESAISLYQENLGLPITG 123

Query: 101 SLNAETLSQLATPR---HDHYHQLS----HAVPHYSFFPGRPRWPPTKYHLTYEFLPN-- 160
            L+  T++ ++ PR    D +  ++    H   HY++F G+P+W   +  LTY       
Sbjct: 124 RLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHK 183

Query: 161 ----THADAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGT 220
               T  D K    RAF+ W+      F    +   ADLKIGFY G+HGDG PFDG  GT
Sbjct: 184 LDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGT 243

Query: 221 LAHAFTPTDGRVHFDSTEKWVV-----GAVRGRFDLETVALHEIGHLLGLGHSRVKNAIM 280
           LAHAF P +GR+H D+ E W+V     G+     DLE+VA HEIGHLLGLGHS  ++A+M
Sbjct: 244 LAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVM 303

Query: 281 YPTIESGSTK-GLNADDIEGIEVLY 287
           YP++   + K  L  DD+ G+  LY
Sbjct: 304 YPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Cucsa.356580 vs. TrEMBL
Match: A0A0A0M032_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 5.9e-167
Identity = 291/315 (92.38%), Postives = 291/315 (92.38%), Query Frame = 1 

Query: 1   MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYL 60
           MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYL
Sbjct: 1   MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYL 60

Query: 61  NHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR------ 120
           NHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR      
Sbjct: 61  NHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNPDI 120

Query: 121 ------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADA 180
                             HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADA
Sbjct: 121 LNEATGRMLLENNNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADA 180

Query: 181 KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTD 240
           KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTD
Sbjct: 181 KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTD 240

Query: 241 GRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNA 292
           GRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNA
Sbjct: 241 GRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNA 300

[Close]
BLAST of Cucsa.356580 vs. TrEMBL
Match: A0A0A0M0J6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.3e-133
Identity = 241/313 (77.00%), Postives = 255/313 (81.47%), Query Frame = 1 

Query: 1   MGSKPLAILLFIS--ILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYG 60
           MG+KPLAILL I    LPLCFSLPL QVS  +FLNDLQG KKGDNVKGISKLKNFFR YG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR---- 120
           YLNHQIN TGHLID DA+D FDDR ESA+KTYQQYFHLN TGSLNAETLSQLATPR    
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 --------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA 180
                               HDH+H L HAV HY+FFPGR RWP TKY LTY FLP T A
Sbjct: 121 DIINETTGRMLSEDIDNVSSHDHHH-LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRA 180

Query: 181 DAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTP 240
           DAKAPV RAFATWAR+THFKF+L TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF P
Sbjct: 181 DAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240

Query: 241 TDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGL 288
           TDGR H+DSTEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I+SGSTKGL
Sbjct: 241 TDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGL 300

[Close]
BLAST of Cucsa.356580 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.0e-83
Identity = 162/317 (51.10%), Postives = 200/317 (63.09%), Query Frame = 1 

Query: 1   MGSKPLAI--LLFISILPLCFSLPL-------RQVSRLSFLNDLQGSKKGDNVKGISKLK 60
           M SKP AI  +  I ++ L  S  L        + S   FL  LQG  KGDN+KGI  LK
Sbjct: 1   MASKPFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLK 60

Query: 61  NFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLA 120
            +   +GYL+++         H  DD FDD  E A+KTYQ  +HLN TG L++ET++++ 
Sbjct: 61  KYLENFGYLSYKNQ------SHSNDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMM 120

Query: 121 TPR--------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPN 180
            PR                    H H     H V HY FFPG PRWP +KYHLTY FLP 
Sbjct: 121 MPRCGVADIINGTTRMQSSNKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPG 180

Query: 181 THADAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHA 240
           T   A  PV +AF TWA +THF+F+   + R AD+ IGF+RG+HGDG PFDG GGTLAHA
Sbjct: 181 TPNQAMEPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHA 240

Query: 241 FTPTDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGST 289
           F P DGR H+D  E W VGA +G FD+ETVALHEIGHLLGLGHS V+ AIM+P+I+SG+T
Sbjct: 241 FAPQDGRFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGAT 300

[Close]
BLAST of Cucsa.356580 vs. TrEMBL
Match: S1SMU3_THECC (Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.8e-78
Identity = 159/300 (53.00%), Postives = 190/300 (63.33%), Query Frame = 1 

Query: 14  ILPLCFSLPL-----RQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTG 73
           +LPL F   L     ++     FL  LQG  KGD VK I KLK +  ++GYL++  N T 
Sbjct: 16  VLPLLFQATLADSKDKKPYPFDFLKHLQGCHKGDKVKDIRKLKKYLEQFGYLSYSKNKT- 75

Query: 74  HLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR-------------- 133
               H  DD FDD  ESA+KTYQ  FHLNS G+L+ ET+S++  PR              
Sbjct: 76  ----HANDDDFDDLLESAIKTYQLNFHLNSNGALDTETVSKMMMPRCGVADIINGTSGMR 135

Query: 134 --HDHYHQLS-----HAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRAFATW 193
                 H+ +     H V HY+FFP  PRWPP+K HLTY FLP T ADA  PV  AF TW
Sbjct: 136 SGKKKPHRAAGSKSIHEVSHYAFFPRSPRWPPSKSHLTYAFLPGTRADAVNPVAGAFQTW 195

Query: 194 ARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKW 253
           A +THF+FS   N R AD+ IGF R +HGDG PFDG GGTLAHAF PT GR H+D+ E W
Sbjct: 196 AANTHFRFSRIDNYRDADITIGFQRRDHGDGNPFDGPGGTLAHAFAPTLGRFHYDADETW 255

Query: 254 VVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNADDIEGIEVLYN 288
            V A  G   LETVALHEIGHLLGLGHS ++NAIMYP+I +G++KGL  DDIEGI+ LYN
Sbjct: 256 SVSARPGTMHLETVALHEIGHLLGLGHSSIENAIMYPSITAGTSKGLARDDIEGIKALYN 310

[Close]
BLAST of Cucsa.356580 vs. TrEMBL
Match: F6HV37_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0066g01970 PE=3 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-78
Identity = 150/280 (53.57%), Postives = 183/280 (65.36%), Query Frame = 1 

Query: 27  SRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESA 86
           S   F+ DLQG  KGD V+GI KLK +  ++GYL++  +       H  DD FDD  ESA
Sbjct: 38  SPFEFIKDLQGCHKGDKVEGIHKLKKYLGQFGYLSYSHS---KYHTHANDDDFDDLVESA 97

Query: 87  VKTYQQYFHLNSTGSLNAETLSQLATPR-------------------HDHYHQLSHAVPH 146
           +KTYQ  +HLN+TGSL++ET+SQ+  PR                   H H H     V H
Sbjct: 98  IKTYQTNYHLNATGSLDSETVSQMVKPRCGAADIINGTNWMRSGKKGHHHGHGSLRTVAH 157

Query: 147 YSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRAFATWARHTHFKFSLATNSRRADLK 206
           YSFF G PRWPP+K +LTY FLP T + A +PV+RAF  W   THF F    +   AD+ 
Sbjct: 158 YSFFSGSPRWPPSKTYLTYAFLPGTPSWAMSPVSRAFGQWDSATHFTFGSIQDHTSADMT 217

Query: 207 IGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKWVVGAVRGRFDLETVALHEIG 266
           I F+R +HGDGYPFDG GGT+AHAF PT+GR H+D  E W +GAV    DLETVALHEIG
Sbjct: 218 ISFHRLDHGDGYPFDGPGGTIAHAFAPTNGRFHYDGDETWSIGAVPNAMDLETVALHEIG 277

Query: 267 HLLGLGHSRVKNAIMYPTIESGSTKGLNADDIEGIEVLYN 288
           HLLGLGHS V+NAIM+P+I SG TKGL+ DDI+GI  LYN
Sbjct: 278 HLLGLGHSSVQNAIMFPSISSGVTKGLHEDDIQGISALYN 314

[Close]
BLAST of Cucsa.356580 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 220.7 bits (561), Expect = 1.2e-57
Identity = 130/314 (41.40%), Postives = 173/314 (55.10%), Query Frame = 1 

Query: 8   ILLFISILPLC--FSLPLRQVSRLSFLNDLQ----------GSKKGDNVKGISKLKNFFR 67
           ++ F ++ P+   F   +  +  L FLN  Q          G   G+N+ G+SKLK +FR
Sbjct: 9   LIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYFR 68

Query: 68  RYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRH 127
           R+GY+    N T         D FDD  +SA+ TYQ+ F+L  TG L++ TL Q+  PR 
Sbjct: 69  RFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRC 128

Query: 128 DHYH-----------QLSHAVPHYSFFPGRPRWPPTKYHLTYEFLP--NTHADAKAPVTR 187
            +             ++      YSFFPG+PRWP  K  LTY F P  N   + K   +R
Sbjct: 129 GNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSR 188

Query: 188 AFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFD 247
           AF  WA  T   F+ + +  RAD+ IGF+ G HGDG PFDG+ GTLAHA +P  G +H D
Sbjct: 189 AFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLD 248

Query: 248 STEKWVV--GAVRGRF-------DLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTK- 287
             E W++  G +  R        DLE+VA+HEIGHLLGLGHS V++AIM+P I  G  K 
Sbjct: 249 GDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKV 308

[Close]
BLAST of Cucsa.356580 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 210.3 bits (534), Expect = 1.6e-54
Identity = 119/298 (39.93%), Postives = 166/298 (55.70%), Query Frame = 1 

Query: 23  LRQVSRLSF--LNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFD 82
           LR  +R+ +   ++  G   G NV G+ ++K +F+R+GY+    +        +  D FD
Sbjct: 33  LRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFS-------GNFTDDFD 92

Query: 83  DRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRHDHYHQLS------------------ 142
           D  ++AV+ YQ  F+LN TG L+A T+  +  PR  +   ++                  
Sbjct: 93  DILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFS 152

Query: 143 ----HAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA--DAKAPVTRAFATWARHTHFKFS 202
               HAV  Y+ FPG PRWP  +  LTY F P      + K+  +RAF  W+  T   F+
Sbjct: 153 RTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFT 212

Query: 203 LATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKWVVG------ 262
           L+ +   +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+ H D+ E WVV       
Sbjct: 213 LSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSF 272

Query: 263 -AVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTK-GLNADDIEGIEVLY 287
            +V    DLE+VA+HEIGHLLGLGHS V+ +IMYPTI +G  K  L  DD+EGI+ LY
Sbjct: 273 LSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of Cucsa.356580 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 205.3 bits (521), Expect = 5.1e-53
Identity = 120/291 (41.24%), Postives = 154/291 (52.92%), Query Frame = 1 

Query: 30  SFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKT 89
           SFLN   G   G    G+  LK +F+ +GY+  + N++G+  D      FDD  ++AV+ 
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSGNFTDD-----FDDILKNAVEM 104

Query: 90  YQQYFHLNSTGSLNAETLSQLATPRHDHYH-----------------------QLSHAVP 149
           YQ+ F LN TG L+  TL  +  PR  +                         Q  HAV 
Sbjct: 105 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK 164

Query: 150 HYSFFPGRPRWPPTKYHLTYEFLPNTHA--DAKAPVTRAFATWARHTHFKFSLATNSRRA 209
           HYSFFPG PRWP  +  LTY F P      + K+  +RAF  W   T   F+       +
Sbjct: 165 HYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 224

Query: 210 DLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKWVVG--------AVRGRF 269
           D+ IGFY G HGDG PFDG   TLAHAF+P  G  H D  E W+V         +V    
Sbjct: 225 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 284

Query: 270 DLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTK-GLNADDIEGIEVLY 287
           DLE+VA+HEIGHLLGLGHS V+ +IMYPTI +G  K  L  DD+EG++ LY
Sbjct: 285 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of Cucsa.356580 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 184.1 bits (466), Expect = 1.2e-46
Identity = 105/265 (39.62%), Postives = 145/265 (54.72%), Query Frame = 1 

Query: 41  GDNVKGISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTG 100
           G +V G+S+LK +  R+GY+N    +          D FD   ESA+  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS--------DVFDGPLESAISLYQENLGLPITG 123

Query: 101 SLNAETLSQLATPR---HDHYHQLS----HAVPHYSFFPGRPRWPPTKYHLTYEFLPN-- 160
            L+  T++ ++ PR    D +  ++    H   HY++F G+P+W   +  LTY       
Sbjct: 124 RLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKW--NRDTLTYAISKTHK 183

Query: 161 ----THADAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGT 220
               T  D K    RAF+ W+      F    +   ADLKIGFY G+HGDG PFDG  GT
Sbjct: 184 LDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGT 243

Query: 221 LAHAFTPTDGRVHFDSTEKWVV-----GAVRGRFDLETVALHEIGHLLGLGHSRVKNAIM 280
           LAHAF P +GR+H D+ E W+V     G+     DLE+VA HEIGHLLGLGHS  ++A+M
Sbjct: 244 LAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVM 303

Query: 281 YPTIESGSTK-GLNADDIEGIEVLY 287
           YP++   + K  L  DD+ G+  LY
Sbjct: 304 YPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Cucsa.356580 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 166.0 bits (419), Expect = 3.5e-41
Identity = 103/258 (39.92%), Postives = 135/258 (52.33%), Query Frame = 1 

Query: 47  ISKLKNFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAET 106
           I ++K   ++YGYL           + ++DD     FE A+  YQ+   L  TG  +++T
Sbjct: 50  IPEIKRHLQQYGYLPQ---------NKESDDV---SFEQALVRYQKNLGLPITGKPDSDT 109

Query: 107 LSQLATPRHDHYHQLS------HAVPHYSFFPGRPRWP-PTKYHLTYEFLPNTHADAKAP 166
           LSQ+  PR      +       H    Y +FPGRPRW       LTY F         AP
Sbjct: 110 LSQILLPRCGFPDDVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAP 169

Query: 167 VT------RAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFT 226
                   RAF  WA      F    +   AD+KIGF+ G+HGDG PFDG  G LAH F+
Sbjct: 170 TDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFS 229

Query: 227 PTDGRVHFDSTEKWVVGAVRGR----FDLETVALHEIGHLLGLGHSRVKNAIMYPTIESG 286
           P +GR+H D  E W V     +     DLE+VA+HEIGH+LGLGHS VK+A MYPT++  
Sbjct: 230 PENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPR 289

[Close]
BLAST of Cucsa.356580 vs. NCBI nr
Match: gi|449442789|ref|XP_004139163.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 594.7 bits (1532), Expect = 8.5e-167
Identity = 291/315 (92.38%), Postives = 291/315 (92.38%), Query Frame = 1 

Query: 1   MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYL 60
           MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYL
Sbjct: 1   MGSKPLAILLFISILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYL 60

Query: 61  NHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR------ 120
           NHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR      
Sbjct: 61  NHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNPDI 120

Query: 121 ------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADA 180
                             HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADA
Sbjct: 121 LNEATGRMLLENNNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADA 180

Query: 181 KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTD 240
           KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTD
Sbjct: 181 KAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTD 240

Query: 241 GRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNA 292
           GRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNA
Sbjct: 241 GRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNA 300

[Close]
BLAST of Cucsa.356580 vs. NCBI nr
Match: gi|659085906|ref|XP_008443669.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 489.2 bits (1258), Expect = 5.0e-135
Identity = 243/312 (77.88%), Postives = 258/312 (82.69%), Query Frame = 1 

Query: 1   MGSKPLAILLFISI-LPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGY 60
           MG+K LAILLFIS+ LPLCFSLPL QVS  +FLNDLQG KKGDNVKGISKLKNFFR YGY
Sbjct: 1   MGNKTLAILLFISVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGY 60

Query: 61  LNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR----- 120
           LNHQIN TGHLID DADDTFDDR E A+KTYQQYFHLN TGSLNAET+SQLATPR     
Sbjct: 61  LNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNPD 120

Query: 121 -------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHAD 180
                              HDH+H L HAV HY+FFPGR RWP TKY LTY FLP T AD
Sbjct: 121 IINESTGRMLSEHNNNDSSHDHHH-LPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRAD 180

Query: 181 AKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPT 240
           AKAPVTRAFATWAR+THFKFSL TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF PT
Sbjct: 181 AKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240

Query: 241 DGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLN 288
           DGR H+DSTEKW VGAV+GR+DL+TVALHEIGHLLGLGHS V+NAIMYP I SGSTKGLN
Sbjct: 241 DGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGLN 300

[Close]
BLAST of Cucsa.356580 vs. NCBI nr
Match: gi|449442791|ref|XP_004139164.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 482.3 bits (1240), Expect = 6.2e-133
Identity = 241/313 (77.00%), Postives = 255/313 (81.47%), Query Frame = 1 

Query: 1   MGSKPLAILLFIS--ILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYG 60
           MG+KPLAILL I    LPLCFSLPL QVS  +FLNDLQG KKGDNVKGISKLKNFFR YG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR---- 120
           YLNHQIN TGHLID DA+D FDDR ESA+KTYQQYFHLN TGSLNAETLSQLATPR    
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 --------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA 180
                               HDH+H L HAV HY+FFPGR RWP TKY LTY FLP T A
Sbjct: 121 DIINETTGRMLSEDIDNVSSHDHHH-LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRA 180

Query: 181 DAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTP 240
           DAKAPV RAFATWAR+THFKF+L TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF P
Sbjct: 181 DAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240

Query: 241 TDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGL 288
           TDGR H+DSTEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I+SGSTKGL
Sbjct: 241 TDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGL 300

[Close]
BLAST of Cucsa.356580 vs. NCBI nr
Match: gi|255552736|ref|XP_002517411.1| (PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis])

HSP 1 Score: 315.5 bits (807), Expect = 1.0e-82
Identity = 162/317 (51.10%), Postives = 200/317 (63.09%), Query Frame = 1 

Query: 1   MGSKPLAI--LLFISILPLCFSLPL-------RQVSRLSFLNDLQGSKKGDNVKGISKLK 60
           M SKP AI  +  I ++ L  S  L        + S   FL  LQG  KGDN+KGI  LK
Sbjct: 1   MASKPFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLK 60

Query: 61  NFFRRYGYLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLA 120
            +   +GYL+++         H  DD FDD  E A+KTYQ  +HLN TG L++ET++++ 
Sbjct: 61  KYLENFGYLSYKNQ------SHSNDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMM 120

Query: 121 TPR--------------------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPN 180
            PR                    H H     H V HY FFPG PRWP +KYHLTY FLP 
Sbjct: 121 MPRCGVADIINGTTRMQSSNKNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPG 180

Query: 181 THADAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHA 240
           T   A  PV +AF TWA +THF+F+   + R AD+ IGF+RG+HGDG PFDG GGTLAHA
Sbjct: 181 TPNQAMEPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHA 240

Query: 241 FTPTDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGST 289
           F P DGR H+D  E W VGA +G FD+ETVALHEIGHLLGLGHS V+ AIM+P+I+SG+T
Sbjct: 241 FAPQDGRFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGAT 300

[Close]
BLAST of Cucsa.356580 vs. NCBI nr
Match: gi|470120973|ref|XP_004296560.1| (PREDICTED: metalloendoproteinase 1-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 314.3 bits (804), Expect = 2.2e-82
Identity = 159/301 (52.82%), Postives = 197/301 (65.45%), Query Frame = 1 

Query: 9   LLFISILPLCFSLPL----RQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYGYLNHQI 68
           LL + +  LC++       +  S + FL  L+G  KGD VKGI+ LK +  +YGYLN++ 
Sbjct: 14  LLLVLLTLLCYATASHPHNKSSSPVEFLEHLKGCHKGDKVKGINDLKKYLEKYGYLNYKN 73

Query: 69  NVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPR---------- 128
           +       H  DD FDD  E AVKTYQ  +HL STG+L+++T+S++ TPR          
Sbjct: 74  H------SHSDDDEFDDLLEEAVKTYQINYHLKSTGTLDSKTVSKMTTPRCGVADIINGT 133

Query: 129 -------HDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHADAKAPVTRAFATW 188
                    H+H   H   HYSFF G P+WP +KYHLTY FLPNT ++A   V RAFATW
Sbjct: 134 SSMRSAKKRHHHGSLHTTAHYSFFQGNPKWPSSKYHLTYGFLPNTPSEATGAVARAFATW 193

Query: 189 ARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTPTDGRVHFDSTEKW 248
             +THF FS A +   ADLKIGF RGNHGDG  FDG GGTLAHAF+PT G  H+D+ E W
Sbjct: 194 EGNTHFTFSQAQSYENADLKIGFQRGNHGDGAAFDGPGGTLAHAFSPTHGIFHYDADETW 253

Query: 249 VVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGLNADDIEGIEVLYN 289
            VGAV G  DLETVALHEIGHLLGLGHS VK AIM PT+ +G T+GL+ DD++GI+ LYN
Sbjct: 254 SVGAVPGATDLETVALHEIGHLLGLGHSSVKGAIMEPTLPTGETRGLHGDDVQGIKALYN 308

The following BLAST results are available for this feature:
BLAST of Cucsa.356580 vs. Swiss-Prot
Match NameE-valueIdentityDescription
5MMP_ARATH2.1e-5641.40Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
2MMP_ARATH2.8e-5339.93Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
3MMP_ARATH9.1e-5241.24Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
MEP1_SOYBN1.0e-5043.02Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
1MMP_ARATH2.2e-4539.62Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
BLAST of Cucsa.356580 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0M032_CUCSA5.9e-16792.38Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1[more]
A0A0A0M0J6_CUCSA4.3e-13377.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1[more]
B9RUG6_RICCO7.0e-8351.10Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1[more]
S1SMU3_THECC1.8e-7853.00Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1[more]
F6HV37_VITVI2.3e-7853.57Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0066g01970 PE=3 SV=... [more]
BLAST of Cucsa.356580 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G59970.11.2e-5741.40 Matrixin family protein[more]
AT1G70170.11.6e-5439.93 matrix metalloproteinase[more]
AT1G24140.15.1e-5341.24 Matrixin family protein[more]
AT4G16640.11.2e-4639.62 Matrixin family protein[more]
AT2G45040.13.5e-4139.92 Matrixin family protein[more]
BLAST of Cucsa.356580 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442789|ref|XP_004139163.1|8.5e-16792.38PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659085906|ref|XP_008443669.1|5.0e-13577.88PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|449442791|ref|XP_004139164.1|6.2e-13377.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|255552736|ref|XP_002517411.1|1.0e-8251.10PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis][more]
gi|470120973|ref|XP_004296560.1|2.2e-8252.82PREDICTED: metalloendoproteinase 1-like [Fragaria vesca subsp. vesca][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.356580.1Cucsa.356580.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 140..286
score: 5.0
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 47..110
score: 1.
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 41..114
score: 1.88
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 133..288
score: 8.3
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 160..175
score: 1.0E-30coord: 241..266
score: 1.0E-30coord: 184..212
score: 1.0E-30coord: 274..287
score: 1.0
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 41..288
score: 3.6
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 1..286
score: 8.4E
NoneNo IPR availablePANTHERPTHR10201:SF140F20P5.11 PROTEINcoord: 1..286
score: 8.4E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 130..289
score: 2.15