Lsi05G018620 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi05G018620
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Werner Syndrome-like exonuclease
Location	chr05 : 25849555 .. 25852677 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAAATCCGACAGTGAAACACGGGTCATCCGAAAAAAAATTCCGAATATTAAGTCAAATTTCCCTCCGTCCGGAAAATGTGAGTTCAATTGAATTGGTAATGGAATTTAACTGCGGTAGCAACGGTGGCCGCGCTTCGACGAATCTCTCCACCTGCTCCTCATCTTCTTCGTCGTGGTCGTTCTCCGTTTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTCCACGCAATCGAGGCCATTGAAGCTGCATATCAAGCCACCAGCGCCAAAAAACGACGAGCAAATTCCACACCCGATGAAAATGAGGTCTCGTGCGTTTCTCCCGACACCGGCCGTCGATTGCCTCGTTCCATTCTGTCTCACCAGTCTCCGCATTCTTCTCCCCTATCGCCCTGTCGAGGTACTTTGTTATCTCTATTGTAAAATGTATTGTAGCAGATGCAATGTCTCGAGGTTTTCGAACTACTTTGGGGTTCTAGGGTTTTTTTTTTTGGTGTTGGAATTGAAATTATGAGTATGTCTGTACTATTTTGGTTTTCAGTAATCTCGAAGATGAGATTTCCTGCATTGAATTTTGGTGGCCGAATTATTTACAGTAGAACTGCGATAGAGGTCGATAGAGCGTCCAAGGAGCTGGCAAAGAAAATCGACTCCATGAGGAAAGGAACGGATCAAATTACTATTGGGTTCGACATCGAGTGGAAACCCACATTTAGAAGAGGTCAGCAGTTGATTCGATCAAACAAACATATGCACGGAAATTTTTGAAGTTTTATTTCTTGTTCATGATCTATTTTCATCTATTAGAAAATGCAAATTCTTCTTTCTATTTCTTGTTACACCTTCCTCCCCTCGTCTGTTGCTATTTTACTAAACAATTGATGCCATGACAGTTTTCTTCATTCTCACCCCTGAAGCATCTTAGTTCTGTTTCTCGTAGGAACCAAATCTGTAGTGTCTTAAAGTAACCAGAAATATTATTCGGAATTCCTCCTCAATTTGTTGACTCTTCATTAATTGGAATTTTATTTTTATTAAAATTTTCTCCCCAAGGAATTTATGCCGGACGATAGGATAAATGCATGAAAATGTTTGGTTCACCATCCATTAAATCCAATTATGCGCAACCATATTTGATAAGATTGAAATTGTTGCTGGTTGTTATGAACTGTAAGAAGTCAGTTCTAGGGAGAGTTATGAGAATAGCTCAACTTGGTGAGACATATATATTTAATCAAACGTTCAAAAGTTTAATCTCCCTACTCCCACATGTTGTTGATCTAAAAATGAAAGAGAGAGAGCGAGTTCTTGAAAGAGCAGAAACTGTTTCTTTCTTGCGGTACTTGTTGGTTGTTTAGTGAAATTACGGCTGCGAATTAGACATGGATTCTTGATTTCCCTAGTTCTTTGTATCTTGGTATGGTTGGTGGCTGTTAACTAAACAGAACTTAAGCTAATTACTTCTTCTATCATTCTTACAACCCGTTTAATTGGTCGTGGATTCCAGAAAATCTGTTGATCAAAACTAGAATTCTACTTCTTTGACTCTTTTGAGATTAATTTATATGCAGGTGTTCCACCCGGAAAGGCAGCAGTCATGCAACTGTGCCTGGACAATTCTCAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCGAAGTTTGCAGGCTCTTCTAGAGGATGAAACACTCGGAAAGGTTTGAAACATCACTATAATTGCTTTTGTAAGTTAATTTTCAAGTACTATTTAGAAGTTTTAAGCCATTTTAATCAACCATGCTGAATAAACTTCAGGCTGGAGTTGGCATTGTCAATGATGCTATAAAAGTCTTTAAAGATTATAACGTGTCTATCAAAGCAGTGGACGAGATTTCAGATCTCGCTAATCAAAAGCTTGCTGGAGTTCCCCAAAAATGGAGTCTTGGGTCTCTAACTGAGACACTTATATCAAAAGAGGTGATCTACTACTATTTTTGTTTCTGTATGAGTTAAGCTGTTTGAATTAAAATATTTATTGCATAATGTAAGGAAAAGCTTCACAACTATTTGAAATTACTTTTCAAGTGCTTAAAGAGTTTAATCAAGTCATTCTATACAATTTCTCCATATAGAAATATCTTTCGTAAGAAATTTTTTTTTTCTCTCCTTTTGGTAATCGGGGCATTGCGAGCCCCAACCCCAGAGGTTCTATCCACCTCAAAGTTAGCTCCACAAAACAGTTTTAAATTATTAAAATTTTCTACTTGAAAGATTTTCAAACATTTCAGCTCTTTTACTACTTGGACTTTGAAAAACATTTTATTTTTGAAACTTATGTTATCTAGTCTCTATTGTCTTTACTCTGGAATCTAGTCTTAGAATGTTTCTGAAATCCTAGGTAAAATTTTAAAACTATATACAATTCTCTCTTCTTCATATATAACCCCAAAACCATTCTCCTATATTCAGACTAATTAAGTAAATATTCATAGAGATCTTAAGCTCAGAGATATTTGCAATCTGCTTCTGCAAATCAAATTCTTATCTTTATTCAAATTTATTGAACTTTATAGCTTCAAAAACCCAACAAAATCAGACTTGGGAACTGGGAGATCGCTGTATTATCAAAGGAGCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACATGAGGTATTATCAAATGTCCCTTTTTCTTTTCATGTGGTAATTAGTTCAAAATTTCTTCAAATCTTCACAAGCAAAAAGATGTTTGAAATGACTTTTCAAGTATTAAAAACTATTGTCAAGCACACTTAAAAAAATCATTACAAGAATGATCTTCATCATTGTAATAATCTCAACCTCAGATCTTAAAAGGATTTCCACATGCTCAAAAAGTAGCAGATTCAGAGCCAATAGTGTGATGCTGTTCCTGCCACAATAACATCCAATGCTCGTATGGAGATTGAAGTTTGTGATACACAGCTAAGTTACCCGATTGTTCTAATTAATCTTATTGTTGAAATTGTTTCTTATCTTAAGAAACTAAAATGGTCTAATTAATCTTAAGTTGCCATGCTATTTCAATTTTATGTTGTTTTATAAAAATAGCATTAAATTACAAGTTTAGTCTATAGACTCT

mRNA sequence

CGAAAATCCGACAGTGAAACACGGGTCATCCGAAAAAAAATTCCGAATATTAAGTCAAATTTCCCTCCGTCCGGAAAATGTGAGTTCAATTGAATTGGTAATGGAATTTAACTGCGGTAGCAACGGTGGCCGCGCTTCGACGAATCTCTCCACCTGCTCCTCATCTTCTTCGTCGTGGTCGTTCTCCGTTTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTCCACGCAATCGAGGCCATTGAAGCTGCATATCAAGCCACCAGCGCCAAAAAACGACGAGCAAATTCCACACCCGATGAAAATGAGGTCTCGTGCGTTTCTCCCGACACCGGCCGTCGATTGCCTCGTTCCATTCTGTCTCACCAGTCTCCGCATTCTTCTCCCCTATCGCCCTGTCGAGTAATCTCGAAGATGAGATTTCCTGCATTGAATTTTGGTGGCCGAATTATTTACAGTAGAACTGCGATAGAGGTCGATAGAGCGTCCAAGGAGCTGGCAAAGAAAATCGACTCCATGAGGAAAGGAACGGATCAAATTACTATTGGGTTCGACATCGAGTGGAAACCCACATTTAGAAGAGGTGTTCCACCCGGAAAGGCAGCAGTCATGCAACTGTGCCTGGACAATTCTCAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCGAAGTTTGCAGGCTCTTCTAGAGGATGAAACACTCGGAAAGGCTGGAGTTGGCATTGTCAATGATGCTATAAAAGTCTTTAAAGATTATAACGTGTCTATCAAAGCAGTGGACGAGATTTCAGATCTCGCTAATCAAAAGCTTGCTGGAGTTCCCCAAAAATGGAGTCTTGGGTCTCTAACTGAGACACTTATATCAAAAGAGCTTCAAAAACCCAACAAAATCAGACTTGGGAACTGGGAGATCGCTGTATTATCAAAGGAGCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACATGAGATCTTAAAAGGATTTCCACATGCTCAAAAAGTAGCAGATTCAGAGCCAATAGTGTGATGCTGTTCCTGCCACAATAACATCCAATGCTCGTATGGAGATTGAAGTTTGTGATACACAGCTAAGTTACCCGATTGTTCTAATTAATCTTATTGTTGAAATTGTTTCTTATCTTAAGAAACTAAAATGGTCTAATTAATCTTAAGTTGCCATGCTATTTCAATTTTATGTTGTTTTATAAAAATAGCATTAAATTACAAGTTTAGTCTATAGACTCT

Coding sequence (CDS)

ATGGAATTTAACTGCGGTAGCAACGGTGGCCGCGCTTCGACGAATCTCTCCACCTGCTCCTCATCTTCTTCGTCGTGGTCGTTCTCCGTTTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTCCACGCAATCGAGGCCATTGAAGCTGCATATCAAGCCACCAGCGCCAAAAAACGACGAGCAAATTCCACACCCGATGAAAATGAGGTCTCGTGCGTTTCTCCCGACACCGGCCGTCGATTGCCTCGTTCCATTCTGTCTCACCAGTCTCCGCATTCTTCTCCCCTATCGCCCTGTCGAGTAATCTCGAAGATGAGATTTCCTGCATTGAATTTTGGTGGCCGAATTATTTACAGTAGAACTGCGATAGAGGTCGATAGAGCGTCCAAGGAGCTGGCAAAGAAAATCGACTCCATGAGGAAAGGAACGGATCAAATTACTATTGGGTTCGACATCGAGTGGAAACCCACATTTAGAAGAGGTGTTCCACCCGGAAAGGCAGCAGTCATGCAACTGTGCCTGGACAATTCTCAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCGAAGTTTGCAGGCTCTTCTAGAGGATGAAACACTCGGAAAGGCTGGAGTTGGCATTGTCAATGATGCTATAAAAGTCTTTAAAGATTATAACGTGTCTATCAAAGCAGTGGACGAGATTTCAGATCTCGCTAATCAAAAGCTTGCTGGAGTTCCCCAAAAATGGAGTCTTGGGTCTCTAACTGAGACACTTATATCAAAAGAGCTTCAAAAACCCAACAAAATCAGACTTGGGAACTGGGAGATCGCTGTATTATCAAAGGAGCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACATGAGATCTTAAAAGGATTTCCACATGCTCAAAAAGTAGCAGATTCAGAGCCAATAGTGTGA

Protein sequence

MEFNCGSNGGRASTNLSTCSSSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHAQKVADSEPIV

Homology

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BLAST of Lsi05G018620 vs. Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.3e-76
Identity = 149/293 (50.85%), Postives = 201/293 (68.60%), Query Frame = 1


Query: 22  SSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTP----------DENEV 81
           SSS+W       D  FTE++L AI+AIEA+Y  + +    +++ P           E + 
Sbjct: 2   SSSNW------IDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDP 61

Query: 82  SCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYSRTAIEVDRASK 141
           + +  +  R+LPRSI S  S    PLS CR      FPA+ FGGRI+YS+TA EVD+ + 
Sbjct: 62  NQIPNNIRRQLPRSITSSTSYKRFPLSRCRA---RNFPAMRFGGRILYSKTATEVDKRAM 121

Query: 142 ELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIP 201
           +L K +D+ R  +    +G DIEW+P+FR+GV PGK A +Q+C+D++ C VMHI HSGIP
Sbjct: 122 QLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIP 181

Query: 202 RSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSL 261
           +SLQ L+ED TL K G+GI  D++K+F DY VSIK V+++SDLANQK+ G  +KW L SL
Sbjct: 182 QSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASL 241

Query: 262 TETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHA 305
           TETL+ KEL KPN+IRLGNWE   LSK+QLQYAATDA+ASW+L+++LK  P A
Sbjct: 242 TETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLPDA 284

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BLAST of Lsi05G018620 vs. Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 69/193 (35.75%), Postives = 115/193 (59.59%), Query Frame = 1


Query: 109 PALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKA 168
           P + F G I+YS  + +    S+++   +       ++  +GFDIEW P + +G   GK 
Sbjct: 39  PFMKFNGSIVYSYESNDCSLLSEDIRSSL------LEEDVLGFDIEWPPVYTKG-KTGKV 98

Query: 169 AVMQLCLDNSQCHVMHIIH-SGIPRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKA 228
           A++Q+C+   +C++ HI   +G P+ L+ LLEDE++ K GVGI  D  K+  DY + +K 
Sbjct: 99  ALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVGVGIEGDQWKLMSDYELKLKG 158

Query: 229 VDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATD 288
             E+S++ANQKL    +KW+   L + L  ++L K    R  NW+I +L+++Q  YAATD
Sbjct: 159 FIELSEMANQKLR-CKEKWTFNGLIKHLFKEQLYKRKSYRCSNWDIFLLTEDQKLYAATD 218

Query: 289 AFASWYLHEILKG 301
           A+A   +++ L+G
Sbjct: 219 AYAGLLIYKKLEG 223

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BLAST of Lsi05G018620 vs. Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 4.3e-31
Identity = 71/192 (36.98%), Postives = 117/192 (60.94%), Query Frame = 1


Query: 109 PALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKA 168
           P L F G I+YS  A +    S++++  +      +D   +GFD+EW P + RG   GK 
Sbjct: 45  PFLEFTGSIVYSYDASDCSFLSEDISMSL------SDGDVVGFDMEWPPLYNRG-KLGKV 104

Query: 169 AVMQLCLDNSQCHVMHIIHSGI-PRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKA 228
           A++QLC+  S+C++ H+    + P+ L+ LLE++ + KAGVGI  D  K+ +D+++ +K 
Sbjct: 105 ALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKN 164

Query: 229 VDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATD 288
             E++D+AN+KL    + WSL SL + L+ K+L K   IR  NW    L+++Q  YAATD
Sbjct: 165 FVELTDVANKKLK-CTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATD 224

Query: 289 AFASWYLHEILK 300
           A+A + ++  L+
Sbjct: 225 AYAGFIIYRNLE 228

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BLAST of Lsi05G018620 vs. Swiss-Prot
Match: WRN_MOUSE (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus GN=Wrn PE=1 SV=3)

HSP 1 Score: 131.7 bits (330), Expect = 1.4e-29
Identity = 68/191 (35.60%), Postives = 117/191 (61.26%), Query Frame = 1


Query: 109 PALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKA 168
           P L F G I+YS  A +    S++++ ++      +D   +GFD+EW P ++ G    + 
Sbjct: 39  PFLEFPGSIVYSYEASDCSFLSEDISMRL------SDGDVVGFDMEWPPIYKPG-KRSRV 98

Query: 169 AVMQLCLDNSQCHVMHIIHSGI-PRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKA 228
           AV+QLC+  S+C++ HI    + P+ L+ LLE++++ KAGVGI  D  K+ +D++V +++
Sbjct: 99  AVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLES 158

Query: 229 VDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATD 288
             E++D+AN+KL    + WSL  L + ++ K+L K   IR  NW    L+++Q  YAATD
Sbjct: 159 FVELTDVANEKLK-CAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD 218

Query: 289 AFASWYLHEIL 299
           A+A   +++ L
Sbjct: 219 AYAGLIIYQKL 221

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BLAST of Lsi05G018620 vs. Swiss-Prot
Match: WRNXO_DROGR (Werner Syndrome-like exonuclease OS=Drosophila grimshawi GN=WRNexo PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.4e-18
Identity = 70/247 (28.34%), Postives = 123/247 (49.80%), Query Frame = 1


Query: 54  ATSAKKRRANSTP----DENEVSCVSPDTGR--RLPRSILSHQSPHSSPLSPCRVISKMR 113
           AT+  KR+   TP    D+      +P   R  R+ RS+ S       P SP + I K +
Sbjct: 40  ATNNNKRKNQDTPEMIKDKENAESENPPKRRSSRVTRSMRS--MAEDGPASPEKEIPK-K 99

Query: 114 FPALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGK 173
            P + + G I Y   + E+  ++ E+ + ++  +   D + + FD+EW  +F+ G  PGK
Sbjct: 100 LPFIKYSGAIKYFTESQEIAASADEVMQWVEK-QINMDVVPMAFDMEWPFSFQTG--PGK 159

Query: 174 AAVMQLCLDNSQCHVMHIIH-SGIPRSLQALLEDETLGKAGVGIVNDAIKVFKDY-NVSI 233
           ++V+Q+C+D   C+V  +   + IP +L AL+    +   GV I  D  K+ +D+  +S 
Sbjct: 160 SSVIQICVDERCCYVYQLSKLNKIPAALAALINHPKVRLHGVNIKADFRKLERDFPEMSA 219

Query: 234 KA-VDEISDLAN--QKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQ 290
           +  +++  DL     ++     +WSL  L   +  K + K  K+R+  W +  L + QL 
Sbjct: 220 EPLIEKCVDLGVWCNQVCETGGRWSLERLANFIAKKAMDKSKKVRMSKWHVIPLDENQLM 279

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BLAST of Lsi05G018620 vs. TrEMBL
Match: A0A0A0L9B9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129680 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.0e-144
Identity = 258/313 (82.43%), Postives = 282/313 (90.10%), Query Frame = 1


Query: 1   MEFNCGSNGGRASTNLSTCSSSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKR 60
           MEF   SN   +ST LS CSSS   WSFS SEFDQPFTEQQL AIEAIEAAYQ+TSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RANSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYS 120
           R NSTPD NEVSCVSPDTGRRLPRSI SHQSP  SPLSPCRV SKMRFPALN+GGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQC 180
           RT  EVDRAS+ELAKKI+S RK  DQITIGFDIEW+P+F+RGVPPGKAAVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLA 240
           HVMHIIHSGIP+SLQALLED+TL KAGVGI +DA KVFK+YNVS+K ++EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKG 300
           GVP+KW L +LTETLISKELQKP++IRLGNWE+AVLSK+QLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVADSEPI 314
           FPH +KVADSEPI
Sbjct: 301 FPHVEKVADSEPI 313

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BLAST of Lsi05G018620 vs. TrEMBL
Match: A0A067L4A5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01087 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.3e-84
Identity = 165/294 (56.12%), Postives = 212/294 (72.11%), Query Frame = 1


Query: 23  SSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTPDENEVSCVSPD----- 82
           SSS   S+SE+DQP T+++L AI+AIEA++Q  +      +S+      S +  D     
Sbjct: 23  SSSPFPSISEWDQPLTDEELQAIDAIEASFQRPALTTTTPSSSAIRKRQSTLQNDHNLQK 82

Query: 83  TGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYSRTAIEVDRASKELAKKI 142
           T R+LP S+L    P S  LSPC+   KMR+P++ FGGRI+YSRT ++V+ A+ EL   +
Sbjct: 83  TRRQLPNSVLGLSRPFS--LSPCQANIKMRYPSMKFGGRILYSRTPMDVEEAANELLFSL 142

Query: 143 DSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQAL 202
           ++ R  TDQ+ IG DIEW+PTFRRGV PGKAAV+QLC     C+VMHIIHSGI ++LQ L
Sbjct: 143 EAKRGETDQVAIGLDIEWRPTFRRGVLPGKAAVLQLCSGTDDCYVMHIIHSGITKTLQFL 202

Query: 203 LEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLIS 262
           LED TL K GVGI  D+ K+FKDYN+S KAV+++S +AN+KL G P+ WSL SLTE L+ 
Sbjct: 203 LEDATLLKVGVGIRGDSAKIFKDYNISTKAVEDLSYVANKKLGGEPKNWSLQSLTEKLVC 262

Query: 263 KELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHAQKVADSE 312
           KELQKPNKIRLGNWE+ VLSKEQLQYAATDAFASW L ++LK  P A+  AD +
Sbjct: 263 KELQKPNKIRLGNWEVDVLSKEQLQYAATDAFASWQLCQVLKSLPDAKVAADEK 314

[Close]

BLAST of Lsi05G018620 vs. TrEMBL
Match: V4RJL2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005507mg PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.5e-78
Identity = 148/279 (53.05%), Postives = 200/279 (71.68%), Query Frame = 1


Query: 34  DQPFTEQQLHAIEAIEAAYQATSAKKRRANSTPDENEVSCVSPDTGRRLPRSILSHQSPH 93
           D+P TEQ+L  +EAIEAAY+ +  +KR+       N        T RRLP S+++  S  
Sbjct: 13  DEPITEQELEIVEAIEAAYELSITRKRQLRPPVHNNHTHRPMSITSRRLPSSLVASPSSP 72

Query: 94  SSPLSPCRVIS-KMRFPALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQITIGFD 153
           S  LS C+  + ++++PA+ FGG+I+YSRT+ EV+ A+ EL + +++ +    Q+ +GFD
Sbjct: 73  SFSLSHCQGANMRLKYPAMRFGGQILYSRTSTEVEMAAIELRRILEANKSEAGQVVVGFD 132

Query: 154 IEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQALLEDETLGKAGVGIVN 213
           IEWKPTFR+GV P KAAVMQ+C D++ C+VM IIHSGIP SLQ LLED T+ K GVGI +
Sbjct: 133 IEWKPTFRKGVLPRKAAVMQICRDSNHCYVMQIIHSGIPPSLQLLLEDSTILKVGVGIGS 192

Query: 214 DAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWE 273
           DA KV++DYNVS+KA +++S LA  K+ G  QKW L SLTE L+ KEL+KPN+IRLGNWE
Sbjct: 193 DAGKVYRDYNVSVKACEDLSYLAKHKIGGDSQKWGLASLTEMLVCKELKKPNRIRLGNWE 252

Query: 274 IAVLSKEQLQYAATDAFASWYLHEILKGFPHAQKVADSE 312
             VLSK+QL YAATDAFASW+L+++LK  P   K A  +
Sbjct: 253 ADVLSKDQLLYAATDAFASWHLYQVLKSLPEPVKDATDQ 291

[Close]

BLAST of Lsi05G018620 vs. TrEMBL
Match: A0A022QVB5_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a024384mg PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-77
Identity = 154/286 (53.85%), Postives = 201/286 (70.28%), Query Frame = 1


Query: 29  SVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTPDENEVSCVSP-DTGRRLPRSIL 88
           S S++D P T++ L AI++  ++  ATSA   +     D +  S  SP    RRLP S+ 
Sbjct: 8   SDSDWDYPLTDEDLRAIDSAFSSAAATSAHPPKRRHDRDRSGASADSPPQIRRRLPDSLF 67

Query: 89  SHQSPHSSPLSPCRVISKMRFPALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTDQI 148
             Q  ++   S   +I   +   +NFGGRIIYSRT  EV+RA+KEL   +++ ++  +Q 
Sbjct: 68  VFQQQNNRNNSAAGIILVEQ--EINFGGRIIYSRTVEEVERAAKELLDFVEAKKRNEEQC 127

Query: 149 TIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQALLEDETLGKAG 208
            +G DIEW+PTFRRGVPPGKAAV+Q+C  N+ CHVMHIIHSGIP  LQ LLED T  K G
Sbjct: 128 ILGLDIEWRPTFRRGVPPGKAAVLQICGHNNCCHVMHIIHSGIPEKLQILLEDRTSLKVG 187

Query: 209 VGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIR 268
           V I NDA KV +DYNVS+ A+ E+SDLANQKL GVP+KWSL SLTETLI ++L KP+KIR
Sbjct: 188 VCIANDASKVLQDYNVSVSALAELSDLANQKLGGVPKKWSLSSLTETLICRQLPKPSKIR 247

Query: 269 LGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHAQKVADSEPI 314
           LGNWE+ VLSKEQL+YAATDA+ SWYL+++LK +P +     +E +
Sbjct: 248 LGNWEVDVLSKEQLKYAATDAYISWYLYQVLKTYPDSAGNTSAETV 291

[Close]

BLAST of Lsi05G018620 vs. TrEMBL
Match: A0A061EJI5_THECC (Werner syndrome-like exonuclease, putative OS=Theobroma cacao GN=TCM_020232 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.1e-77
Identity = 153/281 (54.45%), Postives = 201/281 (71.53%), Query Frame = 1


Query: 31  SEFDQPFTEQQLHAIEAIEAAYQATSAKK-----RRANSTPDENEVSCVSPDTGRRLPRS 90
           +++DQ FTE+ + A++A+EA    +S+       ++ NS   + +    +P+T R+LP S
Sbjct: 9   TDWDQNFTEEVIQAMDAVEATLHPSSSSSPCSLVKKCNSIGHQEK----NPETRRQLPHS 68

Query: 91  ILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYSRTAIEVDRASKELAKKIDSMRKGTD 150
           I+S        LS  +  SK R+PAL FGG+I+YSRT  EV++A+ EL K +   +K T 
Sbjct: 69  IVSPPPSFPFSLSRRQAYSKSRYPALRFGGQILYSRTRSEVEKAAMELLKTLKIKKKETG 128

Query: 151 QITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQALLEDETLGK 210
           Q+T+GFDIEWKP+F+RG+ PGKAAVMQ+C D S C+VMHIIHSGIP SL+ LLED T+ K
Sbjct: 129 QVTVGFDIEWKPSFQRGILPGKAAVMQICCDTSYCYVMHIIHSGIPPSLRFLLEDSTVIK 188

Query: 211 AGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNK 270
            GV I  DA+KVF DYNVS+KAV+++S +ANQKL    Q W L SLTETL+ KEL KP  
Sbjct: 189 VGVAIGGDAVKVFNDYNVSVKAVEDLSYVANQKLNRDCQSWGLASLTETLVCKELPKPRT 248

Query: 271 IRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHAQK 307
           IRLGNWE+  LSKEQLQYAATDAFASW+L+++LK  P A K
Sbjct: 249 IRLGNWELYPLSKEQLQYAATDAFASWHLYQVLKILPDAVK 285

[Close]

BLAST of Lsi05G018620 vs. TAIR10
Match: AT4G13870.2 (AT4G13870.2 Werner syndrome-like exonuclease)

HSP 1 Score: 286.6 bits (732), Expect = 1.9e-77
Identity = 149/293 (50.85%), Postives = 201/293 (68.60%), Query Frame = 1


Query: 22  SSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTP----------DENEV 81
           SSS+W       D  FTE++L AI+AIEA+Y  + +    +++ P           E + 
Sbjct: 2   SSSNW------IDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDP 61

Query: 82  SCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYSRTAIEVDRASK 141
           + +  +  R+LPRSI S  S    PLS CR      FPA+ FGGRI+YS+TA EVD+ + 
Sbjct: 62  NQIPNNIRRQLPRSITSSTSYKRFPLSRCRA---RNFPAMRFGGRILYSKTATEVDKRAM 121

Query: 142 ELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIP 201
           +L K +D+ R  +    +G DIEW+P+FR+GV PGK A +Q+C+D++ C VMHI HSGIP
Sbjct: 122 QLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIP 181

Query: 202 RSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSL 261
           +SLQ L+ED TL K G+GI  D++K+F DY VSIK V+++SDLANQK+ G  +KW L SL
Sbjct: 182 QSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASL 241

Query: 262 TETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHA 305
           TETL+ KEL KPN+IRLGNWE   LSK+QLQYAATDA+ASW+L+++LK  P A
Sbjct: 242 TETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLPDA 284

[Close]

BLAST of Lsi05G018620 vs. TAIR10
Match: AT4G13885.1 (AT4G13885.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-13
Identity = 32/45 (71.11%), Postives = 39/45 (86.67%), Query Frame = 1


Query: 260 LQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHA 305
           L KPN+IRLGNWE+  LSK+QLQYAATDA+ASW+LH++LK  P A
Sbjct: 215 LLKPNRIRLGNWEVHPLSKQQLQYAATDAYASWHLHQVLKDLPDA 259

[Close]

BLAST of Lsi05G018620 vs. TAIR10
Match: AT1G56310.1 (AT1G56310.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 55.1 bits (131), Expect = 9.2e-08
Identity = 44/186 (23.66%), Postives = 84/186 (45.16%), Query Frame = 1


Query: 136 KIDSMRKGTDQI----TIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIH---- 195
           +++ +RK T  +     +G D EWKP + +G    K ++MQ+  D ++  ++ +I     
Sbjct: 364 EVNELRKATSFLEGCRVVGIDCEWKPNYIKGSKQNKVSIMQIGSD-TKIFILDLIKLYND 423

Query: 196 --SGIPRSLQALLEDETLGKAGVGIVNDA---------IKVFKDYNVSIKAVDEISDLAN 255
               +   L  +L+ ++  K G     D          +K F+ Y++ +    +I ++ N
Sbjct: 424 ASEILDNCLSHILQSKSTLKLGYNFQCDIKQLALSYGDLKCFERYDMLL----DIQNVFN 483

Query: 256 QKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHE 303
           +   G      L  LT+ ++   L K    R  +WE   LS+ QL+YAA DA    ++  
Sbjct: 484 EPFGG------LAGLTKKILGVSLNKTR--RNSDWEQRPLSQNQLEYAALDAAVLIHIFR 536

[Close]

BLAST of Lsi05G018620 vs. TAIR10
Match: AT2G36110.1 (AT2G36110.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 52.8 bits (125), Expect = 4.6e-07
Identity = 48/185 (25.95%), Postives = 85/185 (45.95%), Query Frame = 1


Query: 113 FGGRIIYSRTAIEVDRASKELAKKIDSMR-----KGTDQITIGFDIEWKPTFRRGVPPGK 172
           FG R+I     + V   +  + + I S+R     + +  + +G D++W P    G     
Sbjct: 22  FGERLI-----VTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTP----GGSDPP 81

Query: 173 AAVMQLCLDNSQCHVMHIIH-SGIPRSLQALLEDETLGKAGVGIVNDAIKV--FKDYNVS 232
             ++QLC+ N +C ++ + H   IP  L++ LEDET+   GV    D  K+  F+     
Sbjct: 82  PDILQLCVGN-RCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEI 141

Query: 233 IKAVDEISDLANQKLAGVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYA 290
            + +D    L  + L    +K     + E L  K ++K  +I + NW    LS +Q+  A
Sbjct: 142 WRLLDIRHYLPTRLLNSSFEK----IVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQA 192

[Close]

BLAST of Lsi05G018620 vs. TAIR10
Match: AT5G48350.1 (AT5G48350.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 48.5 bits (114), Expect = 8.6e-06
Identity = 47/194 (24.23%), Postives = 83/194 (42.78%), Query Frame = 1


Query: 122 TAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCH 181
           T  E +R    L K   S +    +I IG D E     ++G    K  ++QLC D   C 
Sbjct: 16  TVTEKERDINRLVKTFLSNKNNRKKI-IGLDTE---RVQKGRKLNKTVLLQLC-DGDNCL 75

Query: 182 VMHIIHS----------GIPRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEI 241
           ++ +              +P  L   L        G+GI    +++  ++ ++ K V EI
Sbjct: 76  IVQLPDEDEDEGEGEDDNLPLPLFNFLNLPEFTFVGIGINKTMMRLESEFGLTCKNVVEI 135

Query: 242 SDLANQKLAGVPQKWSLGSLT-------ETLISKELQKPNKIRLGNWEIAVLSKEQLQYA 299
                      P  W+L ++T         ++S E  +P+   L +WE  VL+K Q++ A
Sbjct: 136 G----------PATWNLTNMTTDVKFRISAIVSTE--RPSNAVLEDWEKFVLNKNQIKLA 192

[Close]

BLAST of Lsi05G018620 vs. NCBI nr
Match: gi|449432388|ref|XP_004133981.1| (PREDICTED: Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus])

HSP 1 Score: 520.0 bits (1338), Expect = 2.9e-144
Identity = 258/313 (82.43%), Postives = 282/313 (90.10%), Query Frame = 1


Query: 1   MEFNCGSNGGRASTNLSTCSSSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKR 60
           MEF   SN   +ST LS CSSS   WSFS SEFDQPFTEQQL AIEAIEAAYQ+TSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RANSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYS 120
           R NSTPD NEVSCVSPDTGRRLPRSI SHQSP  SPLSPCRV SKMRFPALN+GGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQC 180
           RT  EVDRAS+ELAKKI+S RK  DQITIGFDIEW+P+F+RGVPPGKAAVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLA 240
           HVMHIIHSGIP+SLQALLED+TL KAGVGI +DA KVFK+YNVS+K ++EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKG 300
           GVP+KW L +LTETLISKELQKP++IRLGNWE+AVLSK+QLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVADSEPI 314
           FPH +KVADSEPI
Sbjct: 301 FPHVEKVADSEPI 313

[Close]

BLAST of Lsi05G018620 vs. NCBI nr
Match: gi|659075776|ref|XP_008438323.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 513.1 bits (1320), Expect = 3.5e-142
Identity = 257/313 (82.11%), Postives = 282/313 (90.10%), Query Frame = 1


Query: 1   MEFNCGSNGGRASTNLSTCSSSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKR 60
           MEF   SN G +ST LS CSSS  SWSFS SEFDQPFTEQQL AIEAIEAAYQ+TSAKKR
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RANSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYS 120
           R NST DEN VSCVSP+TGRRLPRSI S QSPH SPLS CRV SKMRFPAL++GGRIIYS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQC 180
           RT  EVDRAS+ELAKKI+S RKG +QITIGFDIEW+P+F+RGVPPGKAAVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPRSLQALLEDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLA 240
           HVMHIIHSGIP+SLQALLED+TL KAGVGI +DA KVFKDYNVS+K +DEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPQKWSLGSLTETLISKELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKG 300
           GVP+KW L +LTETLISKEL+KP++IRLGNWE+AVLSKEQL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVADSEPI 314
           FPH +KVADSEPI
Sbjct: 301 FPHVEKVADSEPI 313

[Close]

BLAST of Lsi05G018620 vs. NCBI nr
Match: gi|778677795|ref|XP_011650865.1| (PREDICTED: Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus])

HSP 1 Score: 334.7 bits (857), Expect = 1.7e-88
Identity = 168/205 (81.95%), Postives = 180/205 (87.80%), Query Frame = 1


Query: 1   MEFNCGSNGGRASTNLSTCSSSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKR 60
           MEF   SN   +ST LS CSSS   WSFS SEFDQPFTEQQL AIEAIEAAYQ+TSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RANSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVISKMRFPALNFGGRIIYS 120
           R NSTPD NEVSCVSPDTGRRLPRSI SHQSP  SPLSPCRV SKMRFPALN+GGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTAIEVDRASKELAKKIDSMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQC 180
           RT  EVDRAS+ELAKKI+S RK  DQITIGFDIEW+P+F+RGVPPGKAAVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPRSLQALLEDETLGK 206
           HVMHIIHSGIP+SLQALLED+TL K
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSK 205

[Close]

BLAST of Lsi05G018620 vs. NCBI nr
Match: gi|470121931|ref|XP_004297009.1| (PREDICTED: Werner Syndrome-like exonuclease isoform X2 [Fragaria vesca subsp. vesca])

HSP 1 Score: 329.3 bits (843), Expect = 7.2e-87
Identity = 172/288 (59.72%), Postives = 216/288 (75.00%), Query Frame = 1


Query: 21  SSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTPDENEVSCVSPDTGR 80
           +++++ + S  E+D+PFTE+ L AIEA   A  A ++KKRRAN  P +  V+  S    R
Sbjct: 12  ATATATALSDPEWDEPFTEEDLRAIEAAFEA--AATSKKRRAN--PVDEVVTDQSHSARR 71

Query: 81  RLPRSILSHQSPHSSPLSPCRVIS-KMRFPALNFGGRIIYSRTAIEVDRASKELAKKIDS 140
           RLP SIL+ Q P++  LSPCR  + +MR+P L FGG+I YSRT +EV++A+ ++ K + +
Sbjct: 72  RLPSSILALQHPNAFALSPCRQANTRMRYPVLKFGGQITYSRTTVEVEKAAMDILKTVKA 131

Query: 141 MRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQALLE 200
            +    Q  +GFDIEWKPTF+RGVPPGKAAV+Q+C D S CHVMHIIHSGIPRSLQ LLE
Sbjct: 132 KQNEMGQNAVGFDIEWKPTFQRGVPPGKAAVLQICGDTSCCHVMHIIHSGIPRSLQLLLE 191

Query: 201 DETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLISKE 260
             ++ K G GI NDA+KVFKDYNVSIKAV+++S LANQKL G  Q W L SLTE LI K+
Sbjct: 192 SSSIFKVGAGIANDAVKVFKDYNVSIKAVEDLSYLANQKL-GDSQNWGLASLTEKLICKQ 251

Query: 261 LQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHAQKV 308
           L KPNKIRLGNWE   LSKEQLQYAATDAFASWYLHE+L+  P A++V
Sbjct: 252 LLKPNKIRLGNWETKYLSKEQLQYAATDAFASWYLHEVLRSLPDAEEV 294

[Close]

BLAST of Lsi05G018620 vs. NCBI nr
Match: gi|764573042|ref|XP_011462822.1| (PREDICTED: Werner Syndrome-like exonuclease isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 328.9 bits (842), Expect = 9.4e-87
Identity = 172/289 (59.52%), Postives = 216/289 (74.74%), Query Frame = 1


Query: 21  SSSSSWSFSVSEFDQPFTEQQLHAIEAIEAAYQATSAKKRRANSTPDENEVSCVSPDTGR 80
           +++++ + S  E+D+PFTE+ L AIEA   A  A ++KKRRAN  P +  V+  S    R
Sbjct: 12  ATATATALSDPEWDEPFTEEDLRAIEAAFEA--AATSKKRRAN--PVDEVVTDQSHSARR 71

Query: 81  RLPRSILSHQSPHSSPLSPCRVIS--KMRFPALNFGGRIIYSRTAIEVDRASKELAKKID 140
           RLP SIL+ Q P++  LSPCR  +  +MR+P L FGG+I YSRT +EV++A+ ++ K + 
Sbjct: 72  RLPSSILALQHPNAFALSPCRQAANTRMRYPVLKFGGQITYSRTTVEVEKAAMDILKTVK 131

Query: 141 SMRKGTDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPRSLQALL 200
           + +    Q  +GFDIEWKPTF+RGVPPGKAAV+Q+C D S CHVMHIIHSGIPRSLQ LL
Sbjct: 132 AKQNEMGQNAVGFDIEWKPTFQRGVPPGKAAVLQICGDTSCCHVMHIIHSGIPRSLQLLL 191

Query: 201 EDETLGKAGVGIVNDAIKVFKDYNVSIKAVDEISDLANQKLAGVPQKWSLGSLTETLISK 260
           E  ++ K G GI NDA+KVFKDYNVSIKAV+++S LANQKL G  Q W L SLTE LI K
Sbjct: 192 ESSSIFKVGAGIANDAVKVFKDYNVSIKAVEDLSYLANQKL-GDSQNWGLASLTEKLICK 251

Query: 261 ELQKPNKIRLGNWEIAVLSKEQLQYAATDAFASWYLHEILKGFPHAQKV 308
           +L KPNKIRLGNWE   LSKEQLQYAATDAFASWYLHE+L+  P A++V
Sbjct: 252 QLLKPNKIRLGNWETKYLSKEQLQYAATDAFASWYLHEVLRSLPDAEEV 295

The following BLAST results are available for this feature:

BLAST of Lsi05G018620 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WEX_ARATH	3.3e-76	50.85	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1	[more]
WRN_XENLA	1.1e-31	35.75	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=...	[more]
WRN_HUMAN	4.3e-31	36.98	Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2	[more]
WRN_MOUSE	1.4e-29	35.60	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus GN=Wrn PE=1 SV=3	[more]
WRNXO_DROGR	5.4e-18	28.34	Werner Syndrome-like exonuclease OS=Drosophila grimshawi GN=WRNexo PE=3 SV=1	[more]

BLAST of Lsi05G018620 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9B9_CUCSA	2.0e-144	82.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129680 PE=4 SV=1	[more]
A0A067L4A5_JATCU	2.3e-84	56.12	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01087 PE=4 SV=1	[more]
V4RJL2_9ROSI	9.5e-78	53.05	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005507mg PE=4 SV=1	[more]
A0A022QVB5_ERYGU	1.6e-77	53.85	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a024384mg PE=4 SV=1	[more]
A0A061EJI5_THECC	2.1e-77	54.45	Werner syndrome-like exonuclease, putative OS=Theobroma cacao GN=TCM_020232 PE=4...	[more]

BLAST of Lsi05G018620 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G13870.2	1.9e-77	50.85	Werner syndrome-like exonuclease	[more]
AT4G13885.1	1.5e-13	71.11	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G56310.1	9.2e-08	23.66	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	4.6e-07	25.95	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G48350.1	8.6e-06	24.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

BLAST of Lsi05G018620 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432388\|ref\|XP_004133981.1\|	2.9e-144	82.43	PREDICTED: Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus]	[more]
gi\|659075776\|ref\|XP_008438323.1\|	3.5e-142	82.11	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo]	[more]
gi\|778677795\|ref\|XP_011650865.1\|	1.7e-88	81.95	PREDICTED: Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus]	[more]
gi\|470121931\|ref\|XP_004297009.1\|	7.2e-87	59.72	PREDICTED: Werner Syndrome-like exonuclease isoform X2 [Fragaria vesca subsp. ve...	[more]
gi\|764573042\|ref\|XP_011462822.1\|	9.4e-87	59.52	PREDICTED: Werner Syndrome-like exonuclease isoform X1 [Fragaria vesca subsp. ve...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008408	3'-5' exonuclease activity
GO:0003676	nucleic acid binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006139	nucleobase-containing compound metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR012337	RNaseH-like_sf
IPR002562	3'-5'_exonuclease_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G018620.1	Lsi05G018620.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 130..299 score: 4.4
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 117..303 score: 4.2
IPR012337	Ribonuclease H-like domain	GENE3D	G3DSA:3.30.420.10		coord: 120..298 score: 2.6
IPR012337	Ribonuclease H-like domain	unknown	SSF53098	Ribonuclease H-like	coord: 133..305 score: 2.09
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 106..305 score: 1.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi05G018620	Csa3G129680	Cucumber (Chinese Long) v2	culsiB209
Lsi05G018620	CSPI03G10600	Wild cucumber (PI 183967)	cpilsiB207
Lsi05G018620	Cucsa.256660	Cucumber (Gy14) v1	cgylsiB415
Lsi05G018620	CmaCh06G011990	Cucurbita maxima (Rimu)	cmalsiB764
Lsi05G018620	CmoCh06G012360	Cucurbita moschata (Rifu)	cmolsiB757
Lsi05G018620	Cla021399	Watermelon (97103) v1	lsiwmB365
Lsi05G018620	MELO3C006293	Melon (DHL92) v3.5.1	lsimeB378
Lsi05G018620	ClCG05G003120	Watermelon (Charleston Gray)	lsiwcgB335
Lsi05G018620	MELO3C006293.2	Melon (DHL92) v3.6.1	lsimedB396
Lsi05G018620	Cla97C05G083750	Watermelon (97103) v2	lsiwmbB333
Lsi05G018620	Bhi01G000143	Wax gourd	lsiwgoB445
Lsi05G018620	Cp4.1LG08g02610	Cucurbita pepo (Zucchini)	cpelsiB714
Lsi05G018620	CsGy3G010680	Cucumber (Gy14) v2	cgyblsiB196
Lsi05G018620	Carg06308	Silver-seed gourd	carlsiB878
Lsi05G018620	CsaV3_3G010740	Cucumber (Chinese Long) v3	cuclsiB228

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi05G018620	Silver-seed gourd	carlsiB206
Lsi05G018620	Silver-seed gourd	carlsiB983
Lsi05G018620	Watermelon (97103) v2	lsiwmbB341
Lsi05G018620	Bottle gourd (USVL1VR-Ls)	lsilsiB011
Lsi05G018620	Cucurbita maxima (Rimu)	cmalsiB245
Lsi05G018620	Cucurbita maxima (Rimu)	cmalsiB356
Lsi05G018620	Cucurbita moschata (Rifu)	cmolsiB233
Lsi05G018620	Cucurbita moschata (Rifu)	cmolsiB345
Lsi05G018620	Cucurbita moschata (Rifu)	cmolsiB823
Lsi05G018620	Cucurbita pepo (Zucchini)	cpelsiB122
Lsi05G018620	Cucurbita pepo (Zucchini)	cpelsiB506
Lsi05G018620	Wild cucumber (PI 183967)	cpilsiB128
Lsi05G018620	Watermelon (Charleston Gray)	lsiwcgB343