CSPI03G10600 (gene) Wild cucumber (PI 183967)

NameCSPI03G10600
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionWerner syndrome-like exonuclease
LocationChr3 : 8420374 .. 8423996 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAATGGAGCAAAAATCAAACTAGAGGCCTTCCATTTGGGTCACCGTAGCGTAGTGCGAACGAAAATCAAATTTCCGTCCGTCATGAGCACTTAAATGGAATTTCGGGGCAGTAGCAACAGTAGCCATTCTTCGACGGAGCTCTCTGCATGCTCCTCATCTTTTCCGCCGTGGTCGTTCTCCGCATCGGAGTTCGATCAGCCGTTTACAGAGCAACAACTCCAAGCAATTGAGGCCATTGAAGCTGCTTATCAATCCACCAGCGCCAAAAAACGACGACCAAATTCCACGCCCGACGGAAACGAGGTCTCATGCGTTTCTCCCGACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCATCAGTCTCCACGTTTTTCTCCCCTATCGCCCTGTCGAGGTATTTTGTTATTTCTACTGTAAAATGTATTGTAGCAGAGGCGAGGAATTTGGGGAACCATGGGTTGTGGTTTTTGAACTATCTTTTGGGGATCTAGGTTTTTTACTTTTGTGCTGAAATTGGAAGTGCGGGTATGTCTGTACTGTTTTGGGTTTCAGTAAACTCGAAGATGAGGTTTCCTGCATTGAATTATGGTGGCCGAATTATTTACAGTAGAACTGTGAGTGAGGTCGATAGAGCGTCTAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGCAATGGATCAAATTACTATTGGGTTCGACATCGAGTGGAGACCCTCATTTAAAAGAGGTCAGCAGTTGATTCGATTGAACAAACTTACACGTGAATTTTGAAGTTTCATCCACTACTAGAAAACGCAAATGCTTCTTTCGGTTTCTTGTTACACATTTCTCCTCTCATCTGTCGCTATTTCGCCAAACAATTGAATCGAATACGGTTTTCTTTATTCAAAAGTATGTTAGTTGTGTTTCTCGTAGGAACCAAATCTGTAGTGTCTTCAAGAACCAGAAATATTATTTGGATTTCCTCCTCAATGTGATGATCCTTCGTTCATTGAATTTTCTTTTTGTTAAAAGTTTCCCCTCAAGAAATTTATGCTTGACAACAGAACAAATGCATGAAAATATTCATTTCGCTATCAATTAACTTCAATCATGCACAACCTTATTTGATAAGATTGAAATTGTTGCTGGCTCTTATGATCTGCCAGAAGTTATTCTTGGAGAGAGCTATGAGGATAGCTCAAGTGGAAGAAACATATATATTTAACCAAAAAGTTAAAAGGTTTGAATCTCCTAGTTCCCACATGTTGCTGATCTAAAAGCAAAAGAAAAAGAGAAAGAAAGCGATTTATTGTTTCTTTCTTGCGGTATTTGCTGATTGTTTAATGAAATTACGGCTGAGAATTAGACCTTGGATTCTCGATTCCCCCAGTTCTTTGTGACTATAGTTTGTTGCTTTATCTGGTTTTTGTGGTTAAACTCAAATCTTGGTTGGTTGGTGGCTGGTAACTCAACAGAACTTAAGCTAATTACTCCTTCTATCATTCTTACAACCTGTTTAATTTTTTGTGGCTTCCGCAAAATCTGTTGATCAAAACTAGAATTTCTACTTCTTTGACTCTTCTGAGATGAATTTATATGCAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGCTCTTCTAGAGGATGACACACTCAGCAAGGTTTGAAACATCACTATAGTTGCTTCTATAAGTTTGTTTTCATATATTACTTAGAAGTTTTAAGCCATTTTAATCAACCATGTTGAATAAACTTCAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTAAAGAGTATAATGTGTCTGTCAAACCATTGAACGAAATTTCAGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCAAAAGAGGTGGTCTACTACTACTACTTCTGTTTGTATGAGTTTAAGCTGTTTGAATTGAAATATTTCTTATCAATGTAATGAAGTAAAAAGTTCTGGAAATGTTTTGGAATTCTTTTTGAAGTACTTAAAAAAGAGTTTTTAAATTTTGAAAAATAAGTTAAATCTAAGCCCTTGTTTTGTAAATCATTTTCTTTTTGTTCTTGGTTTTTAAAAACTAACCTTCTAAAACCTACTTTCACCTCTAAATTATGTTATATTGCAAACTAATAGGAGCAACTTTTAAAAACAGTTTTTTTTTTGGATTTTGAAAAAGAATCCAAATGTTTCTTCAAAGATAGTGAAACCAGGTAATAATATTGATTGATAAAACAAGTTTAAATTTAAAAAATCAAGTCAGGGTTAACAGAGCTGTAAATTTTCTGCTCGAACGATTTTCAAACATTCCAACCCGTTTACTAGTTACTTCTTAGATGCCGAAAACCAATTTTAATTTCTGAAACTTGTGTAGTTCTCTCTTCTTCATCCATAACCCCAATACTATACTCTTATATTCATATTAATGAAGTAAATGTTCATAGAGATCCTAAGCTCAGAGATATTTACAATTTGCAAATCAAATTTTCACCTTTACAATTGAACATTGTAGCTTCAAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGTGGCTGTGTTGTCAAAGGATCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGGTATTATCAAATGACCCTTTTTTCTCTTTTTATGGTAATTTATTCAGAATTTATTCAAATCTTCATAATAAAAAAGATGTTTGAAATTACCATTTCAATTACCTAAAAACTATTGTTCACTCCCAGCGTTCTTTATCATTGTAACAATCTTAACCTCAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGATGTTGCTGTTACGATAACATTCAATACTCGCCTTGAGATTGAAGGCTCGATTCACAGCTAAGTTATCCAATTGCTCTATTTAATCTTTTTGTTGAAATTGTTTCTTGGCTTAAGAAACTAAAATGATTAATTAATCTTAAGCAGCCATGTTATTTCTATTTTTCGTCACTGATTTTATGTTGAATTACAAATTGTCTATGAAATTAATGTCTAATAGGTCCTTGAAATTTCAGTTTTGTTGCATTAGGTCTTTTTAAAATTCATCACCCAGGTGACACTTATTTTAAAGTTTAATGTCTTATTAGACATAAAATTGTTAAATTTTTTTTTCTCTTTTGAATATATTAGAAACTTATCCATTAATCTTTTACGCTCTAAGTTTCAAAAGTTAGAGCCTATACATGTAAATTAACTTTATTTTTAATTAACCCAAATTGAATTAATTACAAAAGGAGTAGATTTATTTACTCTTGAACCCATAATGCATGGAGTTTAACAAAGGTGTAGCCGTTGAAAGCAATAAACACGAAAACGAGCCTTACATGAAACTGGAAAAGAAACACAATATTCTTACTTTGGCACAATGAGTCGGAATAAATAGAATATACATCTTTCCATTAATACCTTAAGGGCCAAGCCCACAATTCTAACTACAGCTATAAATTTTCAAG

mRNA sequence

ATGGAATTTCGGGGCAGTAGCAACAGTAGCCATTCTTCGACGGAGCTCTCTGCATGCTCCTCATCTTTTCCGCCGTGGTCGTTCTCCGCATCGGAGTTCGATCAGCCGTTTACAGAGCAACAACTCCAAGCAATTGAGGCCATTGAAGCTGCTTATCAATCCACCAGCGCCAAAAAACGACGACCAAATTCCACGCCCGACGGAAACGAGGTCTCATGCGTTTCTCCCGACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCATCAGTCTCCACGTTTTTCTCCCCTATCGCCCTGTCGAGTAAACTCGAAGATGAGGTTTCCTGCATTGAATTATGGTGGCCGAATTATTTACAGTAGAACTGTGAGTGAGGTCGATAGAGCGTCTAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGCAATGGATCAAATTACTATTGGGTTCGACATCGAGTGGAGACCCTCATTTAAAAGAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGCTCTTCTAGAGGATGACACACTCAGCAAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTAAAGAGTATAATGTGTCTGTCAAACCATTGAACGAAATTTCAGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCAAAAGAGCTTCAAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGTGGCTGTGTTGTCAAAGGATCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

Coding sequence (CDS)

ATGGAATTTCGGGGCAGTAGCAACAGTAGCCATTCTTCGACGGAGCTCTCTGCATGCTCCTCATCTTTTCCGCCGTGGTCGTTCTCCGCATCGGAGTTCGATCAGCCGTTTACAGAGCAACAACTCCAAGCAATTGAGGCCATTGAAGCTGCTTATCAATCCACCAGCGCCAAAAAACGACGACCAAATTCCACGCCCGACGGAAACGAGGTCTCATGCGTTTCTCCCGACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCATCAGTCTCCACGTTTTTCTCCCCTATCGCCCTGTCGAGTAAACTCGAAGATGAGGTTTCCTGCATTGAATTATGGTGGCCGAATTATTTACAGTAGAACTGTGAGTGAGGTCGATAGAGCGTCTAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGCAATGGATCAAATTACTATTGGGTTCGACATCGAGTGGAGACCCTCATTTAAAAGAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGCTCTTCTAGAGGATGACACACTCAGCAAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTAAAGAGTATAATGTGTCTGTCAAACCATTGAACGAAATTTCAGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCAAAAGAGCTTCAAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGTGGCTGTGTTGTCAAAGGATCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA
BLAST of CSPI03G10600 vs. Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.5e-73
Identity = 143/284 (50.35%), Postives = 195/284 (68.66%), Query Frame = 1

Query: 29  SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVS---------PDTG 88
           S++  D  FTE++L AI+AIEA+Y  + +     ++ P     + V          P+  
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  RR-LPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYSRTVSEVDRASRELAKKIN 148
           RR LPRSI S  S +  PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K G+GI  D+ K+F +Y VS+K + ++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKGFP 303
           EL KP+RIRLGNWE   LSK QLQYAATDA+ASW+L+++LK  P
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLP 282

BLAST of CSPI03G10600 vs. Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 2.8e-30
Identity = 69/192 (35.94%), Postives = 114/192 (59.38%), Query Frame = 1

Query: 109 PALNYGGRIIYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKA 168
           P L + G I+YS   S+    S +++  ++      D   +GFD+EW P + RG   GK 
Sbjct: 45  PFLEFTGSIVYSYDASDCSFLSEDISMSLS------DGDVVGFDMEWPPLYNRG-KLGKV 104

Query: 169 AVMQLCLENSECHVMHIIHSGI-PQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKP 228
           A++QLC+  S+C++ H+    + PQ L+ LLE+  + KAGVGI  D  K+ +++++ +K 
Sbjct: 105 ALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKN 164

Query: 229 LNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATD 288
             E++D+AN+KL    + W L +L + L+ K+L K   IR  NW    L++DQ  YAATD
Sbjct: 165 FVELTDVANKKLK-CTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATD 224

Query: 289 AFASWYLHEILK 300
           A+A + ++  L+
Sbjct: 225 AYAGFIIYRNLE 228

BLAST of CSPI03G10600 vs. Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.7e-30
Identity = 66/193 (34.20%), Postives = 114/193 (59.07%), Query Frame = 1

Query: 109 PALNYGGRIIYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKA 168
           P + + G I+YS   ++    S ++   +      +++  +GFDIEW P + +G   GK 
Sbjct: 39  PFMKFNGSIVYSYESNDCSLLSEDIRSSL------LEEDVLGFDIEWPPVYTKG-KTGKV 98

Query: 169 AVMQLCLENSECHVMHIIH-SGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKP 228
           A++Q+C+   +C++ HI   +G P+ L+ LLED+++ K GVGI  D  K+  +Y + +K 
Sbjct: 99  ALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVGVGIEGDQWKLMSDYELKLKG 158

Query: 229 LNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATD 288
             E+S++ANQKL    +KW    L + L  ++L K    R  NW++ +L++DQ  YAATD
Sbjct: 159 FIELSEMANQKLR-CKEKWTFNGLIKHLFKEQLYKRKSYRCSNWDIFLLTEDQKLYAATD 218

Query: 289 AFASWYLHEILKG 301
           A+A   +++ L+G
Sbjct: 219 AYAGLLIYKKLEG 223

BLAST of CSPI03G10600 vs. Swiss-Prot
Match: WRN_MOUSE (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus GN=Wrn PE=1 SV=3)

HSP 1 Score: 129.8 bits (325), Expect = 5.2e-29
Identity = 68/191 (35.60%), Postives = 113/191 (59.16%), Query Frame = 1

Query: 109 PALNYGGRIIYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKA 168
           P L + G I+YS   S+    S +++ +++      D   +GFD+EW P +K G    + 
Sbjct: 39  PFLEFPGSIVYSYEASDCSFLSEDISMRLS------DGDVVGFDMEWPPIYKPG-KRSRV 98

Query: 169 AVMQLCLENSECHVMHIIHSGI-PQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKP 228
           AV+QLC+  S+C++ HI    + PQ L+ LLE+ ++ KAGVGI  D  K+ ++++V ++ 
Sbjct: 99  AVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLES 158

Query: 229 LNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATD 288
             E++D+AN+KL    + W L  L + ++ K+L K   IR  NW    L++DQ  YAATD
Sbjct: 159 FVELTDVANEKLK-CAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD 218

Query: 289 AFASWYLHEIL 299
           A+A   +++ L
Sbjct: 219 AYAGLIIYQKL 221

BLAST of CSPI03G10600 vs. Swiss-Prot
Match: WRNXO_DROGR (Werner Syndrome-like exonuclease OS=Drosophila grimshawi GN=WRNexo PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.2e-17
Identity = 81/276 (29.35%), Postives = 131/276 (47.46%), Query Frame = 1

Query: 38  TEQQLQAIEAIEAAYQSTSAKK--------RRPNSTP----DGNEVSCVSPDTGR--RLP 97
           TE+++ A E  E   Q+ +AKK        R+   TP    D       +P   R  R+ 
Sbjct: 18  TEKKISAKE--ERVKQTNAAKKQIATNNNKRKNQDTPEMIKDKENAESENPPKRRSSRVT 77

Query: 98  RSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYSRTVSEV----DRASRELAKKINS 157
           RS+ S       P SP +   K + P + Y G I Y     E+    D   + + K+IN 
Sbjct: 78  RSMRSMAED--GPASPEKEIPK-KLPFIKYSGAIKYFTESQEIAASADEVMQWVEKQIN- 137

Query: 158 TRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIH-SGIPQSLQALL 217
               MD + + FD+EW  SF+ G  PGK++V+Q+C++   C+V  +   + IP +L AL+
Sbjct: 138 ----MDVVPMAFDMEWPFSFQTG--PGKSSVIQICVDERCCYVYQLSKLNKIPAALAALI 197

Query: 218 EDDTLSKAGVGIASDASKVFKEY-NVSVKPLNE----ISDLANQKLAGVPKKWGLRALTE 277
               +   GV I +D  K+ +++  +S +PL E    +    NQ +     +W L  L  
Sbjct: 198 NHPKVRLHGVNIKADFRKLERDFPEMSAEPLIEKCVDLGVWCNQ-VCETGGRWSLERLAN 257

Query: 278 TLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAF 290
            +  K + K  ++R+  W V  L ++QL YAA D +
Sbjct: 258 FIAKKAMDKSKKVRMSKWHVIPLDENQLMYAAIDVY 280

BLAST of CSPI03G10600 vs. TrEMBL
Match: A0A0A0L9B9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129680 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 4.4e-176
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 1

Query: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120
           RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 315
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of CSPI03G10600 vs. TrEMBL
Match: A0A067L4A5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01087 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.4e-81
Identity = 161/314 (51.27%), Postives = 220/314 (70.06%), Query Frame = 1

Query: 6   SSNSSHSSTELSACSSSFPPWSF---SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRP 65
           S  ++++  ++ + S S PP S    S SE+DQP T+++LQAI+AIEA++Q  +     P
Sbjct: 3   SQETTNTECKIVSSSPSLPPSSSPFPSISEWDQPLTDEELQAIDAIEASFQRPALTTTTP 62

Query: 66  NSTPDGNEVSCVSPD-----TGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRI 125
           +S+      S +  D     T R+LP S+     P FS LSPC+ N KMR+P++ +GGRI
Sbjct: 63  SSSAIRKRQSTLQNDHNLQKTRRQLPNSVLGLSRP-FS-LSPCQANIKMRYPSMKFGGRI 122

Query: 126 IYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLEN 185
           +YSRT  +V+ A+ EL   + + R   DQ+ IG DIEWRP+F+RGV PGKAAV+QLC   
Sbjct: 123 LYSRTPMDVEEAANELLFSLEAKRGETDQVAIGLDIEWRPTFRRGVLPGKAAVLQLCSGT 182

Query: 186 SECHVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQ 245
            +C+VMHIIHSGI ++LQ LLED TL K GVGI  D++K+FK+YN+S K + ++S +AN+
Sbjct: 183 DDCYVMHIIHSGITKTLQFLLEDATLLKVGVGIRGDSAKIFKDYNISTKAVEDLSYVANK 242

Query: 246 KLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEI 305
           KL G PK W L++LTE L+ KELQKP++IRLGNWEV VLSK+QLQYAATDAFASW L ++
Sbjct: 243 KLGGEPKNWSLQSLTEKLVCKELQKPNKIRLGNWEVDVLSKEQLQYAATDAFASWQLCQV 302

Query: 306 LKGFPHVEKVADSE 312
           LK  P  +  AD +
Sbjct: 303 LKSLPDAKVAADEK 314

BLAST of CSPI03G10600 vs. TrEMBL
Match: U5FIR6_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0017s08590g PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.3e-78
Identity = 149/279 (53.41%), Postives = 198/279 (70.97%), Query Frame = 1

Query: 33  FDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSP 92
           +DQP T+Q+L+AI+AIEA++QS+S+    P +T   + +        +R       H SP
Sbjct: 1   WDQPLTDQELEAIDAIEASFQSSSSSSSTPAATTPSSSI------INKR-------HSSP 60

Query: 93  RFSPLSP-CRVNSKMRFPALNYGGRIIYSRTVSEVDRASRELAKKINSTRKAMDQITIGF 152
           +     P  R N KMR+PA+ +GG+I+YSRT  EV++A+REL + + + ++ MD++ IGF
Sbjct: 61  QKDQEPPKTRRNVKMRYPAMKFGGQILYSRTSIEVEKAARELLQSLEAEKREMDRVIIGF 120

Query: 153 DIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALLEDDTLSKAGVGIA 212
           DIEW+PSF  GV PGKAAVMQ+C   S CHVMHI HSGI  SLQ LLED  L K G+GI 
Sbjct: 121 DIEWKPSFTTGVLPGKAAVMQICANTSLCHVMHIFHSGITPSLQFLLEDSKLVKVGIGIG 180

Query: 213 SDASKVFKEYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNW 272
            D +KVF++YN SVK + ++S LANQKL G PK WGL+AL + L+ KELQKP++IRLGNW
Sbjct: 181 GDCAKVFRDYNASVKSVEDLSYLANQKLDGKPKTWGLQALAKILVCKELQKPNKIRLGNW 240

Query: 273 EVAVLSKDQLQYAATDAFASWYLHEILKGFPHVEKVADS 311
           +V VLSK+QLQYAATDAFASW L+++LK  P  +   DS
Sbjct: 241 QVDVLSKEQLQYAATDAFASWQLYQVLKSLPDAKDATDS 266

BLAST of CSPI03G10600 vs. TrEMBL
Match: V4RJL2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005507mg PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.0e-76
Identity = 147/280 (52.50%), Postives = 200/280 (71.43%), Query Frame = 1

Query: 34  DQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVSPDTGRRLPRSIF-SHQSP 93
           D+P TEQ+L+ +EAIEAAY+ +  +KR+       N        T RRLP S+  S  SP
Sbjct: 13  DEPITEQELEIVEAIEAAYELSITRKRQLRPPVHNNHTHRPMSITSRRLPSSLVASPSSP 72

Query: 94  RFSPLSPCR-VNSKMRFPALNYGGRIIYSRTVSEVDRASRELAKKINSTRKAMDQITIGF 153
            FS LS C+  N ++++PA+ +GG+I+YSRT +EV+ A+ EL + + + +    Q+ +GF
Sbjct: 73  SFS-LSHCQGANMRLKYPAMRFGGQILYSRTSTEVEMAAIELRRILEANKSEAGQVVVGF 132

Query: 154 DIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALLEDDTLSKAGVGIA 213
           DIEW+P+F++GV P KAAVMQ+C +++ C+VM IIHSGIP SLQ LLED T+ K GVGI 
Sbjct: 133 DIEWKPTFRKGVLPRKAAVMQICRDSNHCYVMQIIHSGIPPSLQLLLEDSTILKVGVGIG 192

Query: 214 SDASKVFKEYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNW 273
           SDA KV+++YNVSVK   ++S LA  K+ G  +KWGL +LTE L+ KEL+KP+RIRLGNW
Sbjct: 193 SDAGKVYRDYNVSVKACEDLSYLAKHKIGGDSQKWGLASLTEMLVCKELKKPNRIRLGNW 252

Query: 274 EVAVLSKDQLQYAATDAFASWYLHEILKGFPHVEKVADSE 312
           E  VLSKDQL YAATDAFASW+L+++LK  P   K A  +
Sbjct: 253 EADVLSKDQLLYAATDAFASWHLYQVLKSLPEPVKDATDQ 291

BLAST of CSPI03G10600 vs. TrEMBL
Match: V4MI48_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10025908mg PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-76
Identity = 150/282 (53.19%), Postives = 204/282 (72.34%), Query Frame = 1

Query: 29  SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR-------RPNSTPDGNEV-SCVSPDTGR 88
           S+ + D+ FTE+QL AI+AIEA+Y S S+          RP+++   +E  S  +P   R
Sbjct: 3   SSWDGDEDFTEEQLLAIDAIEASYNSHSSSSSSSATPTVRPDTSGHRHEAPSSQAPKIRR 62

Query: 89  RLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYSRTVSEVDRASRELAKKINST 148
           +LPRSI S  + +  PLS CR  +   FPA+ +GGRI+YS+T SEVD+ + +L K + + 
Sbjct: 63  QLPRSITSPTAYKRFPLSRCRAKN---FPAMRFGGRILYSKTASEVDKRAMQLLKVLETK 122

Query: 149 RKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALLED 208
           R       +GFDIEWRPSF++GV PGK AV+Q+C+EN+ C V+HIIHSGIPQSLQ L+ED
Sbjct: 123 RDDSGTAIVGFDIEWRPSFRKGVLPGKVAVVQICVENNYCDVIHIIHSGIPQSLQQLIED 182

Query: 209 DTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISKEL 268
            TL K G+GI  D+ K+F ++ VS+K + ++SDLAN+K+ G  KKWGL +LTETL+ KEL
Sbjct: 183 STLVKVGIGIDGDSVKLFHDHGVSIKDVEDLSDLANKKIGGDSKKWGLASLTETLVCKEL 242

Query: 269 QKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKGFP 303
            KP+RIRLGNWEV  LSK+QLQYAATDA+ASW+L+++LK  P
Sbjct: 243 LKPNRIRLGNWEVQPLSKEQLQYAATDAYASWHLYQVLKELP 281

BLAST of CSPI03G10600 vs. TAIR10
Match: AT4G13870.2 (AT4G13870.2 Werner syndrome-like exonuclease)

HSP 1 Score: 276.2 bits (705), Expect = 2.6e-74
Identity = 143/284 (50.35%), Postives = 195/284 (68.66%), Query Frame = 1

Query: 29  SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVS---------PDTG 88
           S++  D  FTE++L AI+AIEA+Y  + +     ++ P     + V          P+  
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  RR-LPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYSRTVSEVDRASRELAKKIN 148
           RR LPRSI S  S +  PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K G+GI  D+ K+F +Y VS+K + ++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKGFP 303
           EL KP+RIRLGNWE   LSK QLQYAATDA+ASW+L+++LK  P
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLP 282

BLAST of CSPI03G10600 vs. TAIR10
Match: AT4G13885.1 (AT4G13885.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 73.2 bits (178), Expect = 3.3e-13
Identity = 32/43 (74.42%), Postives = 37/43 (86.05%), Query Frame = 1

Query: 260 LQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKGFP 303
           L KP+RIRLGNWEV  LSK QLQYAATDA+ASW+LH++LK  P
Sbjct: 215 LLKPNRIRLGNWEVHPLSKQQLQYAATDAYASWHLHQVLKDLP 257

BLAST of CSPI03G10600 vs. TAIR10
Match: AT2G36110.1 (AT2G36110.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-08
Identity = 43/176 (24.43%), Postives = 83/176 (47.16%), Query Frame = 1

Query: 121 RTVSEVDRASRELAKKINSTR-----KAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCL 180
           R +  V   +  + + I+S R     ++   + +G D++W P      P     ++QLC+
Sbjct: 25  RLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGSDPPPD----ILQLCV 84

Query: 181 ENSECHVMHIIH-SGIPQSLQALLEDDTLSKAGVGIASDASKVFK-EYNVSVKPLNEISD 240
            N  C ++ + H   IP+ L++ LED+T++  GV  + D  K+ +  + + +  L +I  
Sbjct: 85  GN-RCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLEIWRLLDIRH 144

Query: 241 LANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAF 290
               +L     +   + + E L  K ++K   I + NW    LS DQ+  A+ D +
Sbjct: 145 YLPTRLLNSSFE---KIVEECLGYKGVRKDKEICMSNWGARSLSHDQIVQASDDVY 192

BLAST of CSPI03G10600 vs. TAIR10
Match: AT1G56310.1 (AT1G56310.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 54.7 bits (130), Expect = 1.2e-07
Identity = 44/186 (23.66%), Postives = 83/186 (44.62%), Query Frame = 1

Query: 136 KINSTRKAMDQI----TIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIH---- 195
           ++N  RKA   +     +G D EW+P++ +G    K ++MQ+   +++  ++ +I     
Sbjct: 364 EVNELRKATSFLEGCRVVGIDCEWKPNYIKGSKQNKVSIMQIG-SDTKIFILDLIKLYND 423

Query: 196 --SGIPQSLQALLEDDTLSKAGVGIASDAS---------KVFKEYNVSVKPLNEISDLAN 255
               +   L  +L+  +  K G     D           K F+ Y++    L +I ++ N
Sbjct: 424 ASEILDNCLSHILQSKSTLKLGYNFQCDIKQLALSYGDLKCFERYDM----LLDIQNVFN 483

Query: 256 QKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHE 303
           +   G      L  LT+ ++   L K  R    +WE   LS++QL+YAA DA    ++  
Sbjct: 484 EPFGG------LAGLTKKILGVSLNKTRRN--SDWEQRPLSQNQLEYAALDAAVLIHIFR 536

BLAST of CSPI03G10600 vs. TAIR10
Match: AT5G48350.1 (AT5G48350.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 49.3 bits (116), Expect = 5.1e-06
Identity = 47/194 (24.23%), Postives = 83/194 (42.78%), Query Frame = 1

Query: 122 TVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECH 181
           TV+E +R    L K   S +    +I IG D E     ++G    K  ++QLC +   C 
Sbjct: 16  TVTEKERDINRLVKTFLSNKNNRKKI-IGLDTE---RVQKGRKLNKTVLLQLC-DGDNCL 75

Query: 182 VMHIIHS----------GIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEI 241
           ++ +              +P  L   L     +  G+GI     ++  E+ ++ K + EI
Sbjct: 76  IVQLPDEDEDEGEGEDDNLPLPLFNFLNLPEFTFVGIGINKTMMRLESEFGLTCKNVVEI 135

Query: 242 SDLANQKLAGVPKKWGLRALT-------ETLISKELQKPDRIRLGNWEVAVLSKDQLQYA 299
                      P  W L  +T         ++S E  +P    L +WE  VL+K+Q++ A
Sbjct: 136 G----------PATWNLTNMTTDVKFRISAIVSTE--RPSNAVLEDWEKFVLNKNQIKLA 192

BLAST of CSPI03G10600 vs. NCBI nr
Match: gi|449432388|ref|XP_004133981.1| (PREDICTED: Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus])

HSP 1 Score: 625.2 bits (1611), Expect = 6.4e-176
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 1

Query: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120
           RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 315
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of CSPI03G10600 vs. NCBI nr
Match: gi|659075776|ref|XP_008438323.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 578.6 bits (1490), Expect = 6.8e-162
Identity = 292/314 (92.99%), Postives = 302/314 (96.18%), Query Frame = 1

Query: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF GSSNS HSSTELSACSSSFP WSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120
           RPNST D N VSCVSP+TGRRLPRSIFS QSP FSPLS CRVNSKMRFPAL+YGGRIIYS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQALLEDDTL+KAGVGIASDASKVFK+YNVS+K L+EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKEL+KPDRIRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 315
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

BLAST of CSPI03G10600 vs. NCBI nr
Match: gi|778677795|ref|XP_011650865.1| (PREDICTED: Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 1.2e-110
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 1

Query: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120
           RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQALLEDDTLSK 206
           HVMHIIHSGIPQSLQALLEDDTLSK
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSK 205

BLAST of CSPI03G10600 vs. NCBI nr
Match: gi|743897752|ref|XP_011042172.1| (PREDICTED: Werner Syndrome-like exonuclease [Populus euphratica])

HSP 1 Score: 317.4 bits (812), Expect = 2.8e-83
Identity = 159/292 (54.45%), Postives = 208/292 (71.23%), Query Frame = 1

Query: 32  EFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSC--------------VSPD 91
           ++D+P T+Q+L+AI+AIEA++QS++      +STP     S                 P 
Sbjct: 34  DWDEPLTDQELEAIDAIEASFQSSTPSSSSSSSTPAATTPSSSIINKRHSSPQKDQEPPK 93

Query: 92  TGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYSRTVSEVDRASRELAKKI 151
           T R+LP SIFS   P FS LSPC+ N KMR+PA+ +GG+I+YSRT  EV++A+REL + +
Sbjct: 94  TRRQLPNSIFSLSKP-FS-LSPCQGNVKMRYPAMKFGGQILYSRTSIEVEKAARELLQSL 153

Query: 152 NSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQAL 211
            + ++  D++ IGFDIEW+PSF  GV PGKAAVMQ+C   S CHVMHI HSGI QSLQ L
Sbjct: 154 EAEKRERDRVVIGFDIEWKPSFTTGVLPGKAAVMQICANTSLCHVMHIFHSGITQSLQFL 213

Query: 212 LEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLIS 271
           LED  L K G+GI  D +KVF++YNVSVK + ++S LANQKL G PK WGL+AL + L+ 
Sbjct: 214 LEDSKLVKVGIGIGGDCAKVFRDYNVSVKSVEDLSYLANQKLDGKPKTWGLQALAKILVC 273

Query: 272 KELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKGFPHVEKVAD 310
           KELQKP++IRLGNW+V VLSK+QLQYAATDAFASW L+++LK  P  +   D
Sbjct: 274 KELQKPNKIRLGNWQVDVLSKEQLQYAATDAFASWQLYQVLKSLPDAKDATD 323

BLAST of CSPI03G10600 vs. NCBI nr
Match: gi|802588809|ref|XP_012071088.1| (PREDICTED: Werner Syndrome-like exonuclease [Jatropha curcas])

HSP 1 Score: 310.5 bits (794), Expect = 3.5e-81
Identity = 161/314 (51.27%), Postives = 220/314 (70.06%), Query Frame = 1

Query: 6   SSNSSHSSTELSACSSSFPPWSF---SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRP 65
           S  ++++  ++ + S S PP S    S SE+DQP T+++LQAI+AIEA++Q  +     P
Sbjct: 3   SQETTNTECKIVSSSPSLPPSSSPFPSISEWDQPLTDEELQAIDAIEASFQRPALTTTTP 62

Query: 66  NSTPDGNEVSCVSPD-----TGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRI 125
           +S+      S +  D     T R+LP S+     P FS LSPC+ N KMR+P++ +GGRI
Sbjct: 63  SSSAIRKRQSTLQNDHNLQKTRRQLPNSVLGLSRP-FS-LSPCQANIKMRYPSMKFGGRI 122

Query: 126 IYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLEN 185
           +YSRT  +V+ A+ EL   + + R   DQ+ IG DIEWRP+F+RGV PGKAAV+QLC   
Sbjct: 123 LYSRTPMDVEEAANELLFSLEAKRGETDQVAIGLDIEWRPTFRRGVLPGKAAVLQLCSGT 182

Query: 186 SECHVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQ 245
            +C+VMHIIHSGI ++LQ LLED TL K GVGI  D++K+FK+YN+S K + ++S +AN+
Sbjct: 183 DDCYVMHIIHSGITKTLQFLLEDATLLKVGVGIRGDSAKIFKDYNISTKAVEDLSYVANK 242

Query: 246 KLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEI 305
           KL G PK W L++LTE L+ KELQKP++IRLGNWEV VLSK+QLQYAATDAFASW L ++
Sbjct: 243 KLGGEPKNWSLQSLTEKLVCKELQKPNKIRLGNWEVDVLSKEQLQYAATDAFASWQLCQV 302

Query: 306 LKGFPHVEKVADSE 312
           LK  P  +  AD +
Sbjct: 303 LKSLPDAKVAADEK 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WEX_ARATH4.5e-7350.35Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1[more]
WRN_HUMAN2.8e-3035.94Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2[more]
WRN_XENLA4.7e-3034.20Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=... [more]
WRN_MOUSE5.2e-2935.60Werner syndrome ATP-dependent helicase homolog OS=Mus musculus GN=Wrn PE=1 SV=3[more]
WRNXO_DROGR1.2e-1729.35Werner Syndrome-like exonuclease OS=Drosophila grimshawi GN=WRNexo PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L9B9_CUCSA4.4e-176100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G129680 PE=4 SV=1[more]
A0A067L4A5_JATCU2.4e-8151.27Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01087 PE=4 SV=1[more]
U5FIR6_POPTR3.3e-7853.41Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0017s08590g P... [more]
V4RJL2_9ROSI1.0e-7652.50Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005507mg PE=4 SV=1[more]
V4MI48_EUTSA1.4e-7653.19Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10025908mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G13870.22.6e-7450.35 Werner syndrome-like exonuclease[more]
AT4G13885.13.3e-1374.42 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT2G36110.12.4e-0824.43 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT1G56310.11.2e-0723.66 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
AT5G48350.15.1e-0624.23 Polynucleotidyl transferase, ribonuclease H-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449432388|ref|XP_004133981.1|6.4e-176100.00PREDICTED: Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus][more]
gi|659075776|ref|XP_008438323.1|6.8e-16292.99PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo][more]
gi|778677795|ref|XP_011650865.1|1.2e-110100.00PREDICTED: Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus][more]
gi|743897752|ref|XP_011042172.1|2.8e-8354.45PREDICTED: Werner Syndrome-like exonuclease [Populus euphratica][more]
gi|802588809|ref|XP_012071088.1|3.5e-8151.27PREDICTED: Werner Syndrome-like exonuclease [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0025623'-5'_exonuclease_dom
IPR012337RNaseH-like_sf
IPR012337RNaseH-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:00084083'-5' exonuclease activity
GO:0003676nucleic acid binding
Vocabulary: Biological Process
TermDefinition
GO:0006139nucleobase-containing compound metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003676 nucleic acid binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G10600.1CSPI03G10600.1mRNA
CSPI03G10600.2CSPI03G10600.2mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 120..203
score: 1.2E-11coord: 205..244
score: 3.
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 118..246
score: 3.03
NoneNo IPR availablePANTHERPTHR136203-5 EXONUCLEASEcoord: 106..249
score: 7.2

The following gene(s) are paralogous to this gene:

None