Lsi05G003000 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi05G003000 vs. Swiss-Prot
Match: LEU12_ARATH (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 3.4e-166
Identity = 292/394 (74.11%), Postives = 335/394 (85.03%), Query Frame = 1

Query: 42  RPLSFRTAAADNVSRIYCSQTESIVSDSGPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDG 101
           R  + RT +     +   S + S  S   P +  RRP YIPNRI DP+YVR+FDTTLRDG
Sbjct: 38  RSQNHRTISLTTAGKFRVSYSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDG 97

Query: 102 EQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDEDGYV 161
           EQSPGA+LT+KEK+DIARQLAKLGVDIIEAGFPAASK+DFEAVK I++ VGN VDE+GYV
Sbjct: 98  EQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYV 157

Query: 162 PVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARNMVR 221
           PVICGLSRCN+ DI TAWEAVKYAKRPRIHTFIATS+IH+K+KL+K++E+VIEIARNMVR
Sbjct: 158 PVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVR 217

Query: 222 FARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVAD 281
           FARSLGCEDVEFSPEDAGRS+RE+LY+ILGEVIKAGATT+NIPDTVG TLP EFG+L+AD
Sbjct: 218 FARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIAD 277

Query: 282 VKSNTPGIENVIISTHCQNDLGLATANTVSGACAGARQV-----------------EVVM 341
           +K+NTPGI+NVIISTHCQNDLGL+TANT+SGA +GARQV                 EVVM
Sbjct: 278 IKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVM 337

Query: 342 ALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGM 401
           A++CRG+HVLGGL TGI+ RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQDGM
Sbjct: 338 AIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGM 397

Query: 402 LKHKGTYEIICPEDIGYERSSDAGIVLGKLRMTH 419
           LKHKGTYEI+ PE+IG ERS+DAGIVLGKL   H
Sbjct: 398 LKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRH 431

BLAST of Lsi05G003000 vs. Swiss-Prot
Match: LEU11_ARATH (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 577.0 bits (1486), Expect = 2.7e-163
Identity = 282/359 (78.55%), Postives = 320/359 (89.14%), Query Frame = 1

Query: 77  RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAA 136
           RP YIPNRI DP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 137 SKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 196
           SK+DFEAVK I++ VGN VDE+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 197 SEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKA 256
           S+IH+++KL+KT+ +VIEIAR+MVRFARSLGCEDVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 257 GATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGACAG 316
           GATT+NIPDTVG TLP EFG+L+ D+K+NTPGIENV+ISTHCQNDLGL+TANT+SGA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 317 ARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGL 376
           ARQ+                 EVVMA++CRG+HVLGGL TGI+ RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 377 NVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMTH 419
             QPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPE+IG ERS+DAGIVLGKL   H
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRH 433

BLAST of Lsi05G003000 vs. Swiss-Prot
Match: LEU1A_SOLPN (2-isopropylmalate synthase A OS=Solanum pennellii GN=IPMSA PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.2e-158
Identity = 272/368 (73.91%), Postives = 320/368 (86.96%), Query Frame = 1

Query: 74  SIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGF 133
           S RRP Y+P++I DP YVR+FDTTLRDGEQSPGA++T KEK+D+ARQLAKLGVDIIEAGF
Sbjct: 31  SNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 90

Query: 134 PAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTF 193
           PA+S+ DFE+VK+I++E+GN  DE+G+VPVICGLSRCN++DI  AWEAVKYAK+PR+HTF
Sbjct: 91  PASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTF 150

Query: 194 IATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEV 253
           IATSEIHMK+KL+ +REQV+E AR+MV +ARSLGCEDVEFSPEDAGRSDREFLY ILGEV
Sbjct: 151 IATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEV 210

Query: 254 IKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGA 313
           IKAGATT+NIPDTVGYT+P EFG+L+ D+K+NTPGIENVIISTHCQNDLGL+TANT++GA
Sbjct: 211 IKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGA 270

Query: 314 CAGARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEY 373
           CAGARQ+                 EVVMAL+CRGE VLGGL+TGIN +HI  +SKMVEEY
Sbjct: 271 CAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSKMVEEY 330

Query: 374 TGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRM 425
           +GL VQPHKAIVGANAFAHESGIHQDGMLKHK TYEII P+D+G  RS++AGIVLGKL  
Sbjct: 331 SGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSG 390

BLAST of Lsi05G003000 vs. Swiss-Prot
Match: LEU1B_SOLPN (2-isopropylmalate synthase B OS=Solanum pennellii GN=IPMSB PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.4e-151
Identity = 269/393 (68.45%), Postives = 319/393 (81.17%), Query Frame = 1

Query: 40  PSRPLSFRTAAADNVSRIYCSQTESIVSDSGPKSSIRR-PPYIPNRIPDPSYVRVFDTTL 99
           P  PL  +T    N        + S  S    + SIRR P Y P+ IPDP+YVR+FDTTL
Sbjct: 20  PKNPL-LQTQTLFNFKPSISKHSNSSFSIPVVRCSIRRIPEYTPSHIPDPNYVRIFDTTL 79

Query: 100 RDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDED 159
           RDGEQSPGA++T KEK+D+ARQ AKLGVDIIEAGFPA+S+ D EAVK+I+KEVGN V E+
Sbjct: 80  RDGEQSPGATMTTKEKLDVARQSAKLGVDIIEAGFPASSEADLEAVKLIAKEVGNGVYEE 139

Query: 160 GYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARN 219
            YVPVICGL+RCN+ DI  AWEAVKYAK+PRIHTFIATSE+HM +KL+ +R+QV+E AR+
Sbjct: 140 EYVPVICGLARCNKKDIDKAWEAVKYAKKPRIHTFIATSEVHMNYKLKMSRDQVVEKARS 199

Query: 220 MVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGEL 279
           MV +ARS+GCEDVEFSPEDAGRSD EFLY ILGEVIKAGATT+NIPDTVGYT+P EFG+L
Sbjct: 200 MVAYARSIGCEDVEFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQL 259

Query: 280 VADVKSNTPGIENVIISTHCQNDLGLATANTVSGACAGARQV-----------------E 339
           +A +K+NTPG+E+VIISTHCQNDLGL+TANT++GACAGARQ+                 E
Sbjct: 260 IAKIKANTPGVEDVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEE 319

Query: 340 VVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 399
           VVMAL+CRGE VLGGL+TGIN +HI ++SKMVE  +GL+VQPHKAIVGANAF HESGIHQ
Sbjct: 320 VVMALKCRGEQVLGGLYTGINTQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQ 379

Query: 400 DGMLKHKGTYEIICPEDIGYERSSDAGIVLGKL 415
           DGMLKHK TYEII PEDIG  R++++GIV GKL
Sbjct: 380 DGMLKHKDTYEIISPEDIGLNRANESGIVFGKL 411

BLAST of Lsi05G003000 vs. Swiss-Prot
Match: LEU1_SOYBN (Probable 2-isopropylmalate synthase OS=Glycine max GN=GMN56 PE=2 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 3.4e-142
Identity = 252/368 (68.48%), Postives = 294/368 (79.89%), Query Frame = 1

Query: 64  SIVSDSGPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAK 123
           S  S   PK S  RP YIPN IPD SYVR+ DTTLRDGEQSPGA++TAKEK+DIARQL K
Sbjct: 6   STPSSQSPKLSHLRPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVK 65

Query: 124 LGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVK 183
           LGVDII+ GFP+AS  DF AVKMI++EVGNAVD+DGYVPVI G  RC E DI TAWEAVK
Sbjct: 66  LGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVK 125

Query: 184 YAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDR 243
           YAKRPR+ T IATS IHM+HKLRK+++QVI+IAR+MV+FARSLGC D++F  EDA RSDR
Sbjct: 126 YAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDR 185

Query: 244 EFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLG 303
           EFLY+ILG VI+AGATT+NI DTVG  +P+E G+L+ D+K NTPGI NVIISTHC NDLG
Sbjct: 186 EFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLG 245

Query: 304 LATANTVSGACAGARQV-----------------EVVMALQCRGEHVLGGLHTGINARHI 363
           LATANT+ GA  GARQ+                 EVVMAL  +G+H L GL+T IN RHI
Sbjct: 246 LATANTIEGARTGARQLEVTINGIGERAGNASLEEVVMALASKGDHALNGLYTRINTRHI 305

Query: 364 FLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSD 415
             TSKMVEEY+G+++QPHK +VGANAF H SGIHQDGMLKHKGTYE I PE+IG++R++ 
Sbjct: 306 LETSKMVEEYSGMHLQPHKPLVGANAFVHASGIHQDGMLKHKGTYETISPEEIGHKRTTR 365

BLAST of Lsi05G003000 vs. TrEMBL
Match: A0A0B0MCM2_GOSAR (2-isopropylmalate synthase 2, chloroplastic-like protein OS=Gossypium arboreum GN=F383_14912 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 2.3e-214
Identity = 409/621 (65.86%), Postives = 469/621 (75.52%), Query Frame = 1

Query: 71  PKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIE 130
           P SS R PPYIPN IPDP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIE
Sbjct: 60  PPSSQRCPPYIPNHIPDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIE 119

Query: 131 AGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRI 190
           AGFPAASK+DFEAVK I+KEVGNAVD +GYVPVICGLSRCNE DI+TAWEAVKYAKRPRI
Sbjct: 120 AGFPAASKDDFEAVKTIAKEVGNAVDGNGYVPVICGLSRCNEKDIKTAWEAVKYAKRPRI 179

Query: 191 HTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQIL 250
           HTFIATS IH+++KLRK++E+V++IARNMVRFARSLGC DVEFSPEDAGRSDREFLY+IL
Sbjct: 180 HTFIATSAIHLEYKLRKSKEEVLDIARNMVRFARSLGCNDVEFSPEDAGRSDREFLYEIL 239

Query: 251 GEVIKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTV 310
           GEVIKAGATT+NIPDTVG T+P EFG+L+AD+K+NTPGIENVIISTHCQNDLGL+TANT+
Sbjct: 240 GEVIKAGATTLNIPDTVGITMPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTI 299

Query: 311 SGACAGARQVEV-----------------VMALQCRGEHVLGGLHTGINARHIFLTSKMV 370
           +GACAGARQVEV                 VMAL+CRGEHVLGGLHTGIN RH        
Sbjct: 300 AGACAGARQVEVTINGIGERAGNASMEEVVMALKCRGEHVLGGLHTGINTRH-------- 359

Query: 371 EEYTGLNVQPHKAIVGANAFAHE-SGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLG 430
                        IV A+    E +G+H   +  HK    I+      +E    +GI   
Sbjct: 360 -------------IVMASKMVEEYTGLH---VQPHKA---IVGANAFAHE----SGI--- 419

Query: 431 KLRMTHENGAKVGTGS-DISSTRNTSFPRLEDLSNVGHLIRRCIICEAFSVQAVPRELMW 490
                H++G     G+ +I S  +    R  +   V   +R      +  +  +  ++  
Sbjct: 420 -----HQDGMLKHKGTYEIISPEDIGLERSNEAGIVLGKLRFLTFTNSMVLCYLELDM-- 479

Query: 491 FFLGQWKCETSVAVLFGRHALKSRLLELGYELDDENLHNVFWRFKAVAEQKKRVTDADLR 550
                      V  + GRHAL  RL ELGYELDD  L N+FWRFK VAEQKKRVTDADL 
Sbjct: 480 --------NVGVGPMGGRHALSDRLKELGYELDDVQLGNIFWRFKEVAEQKKRVTDADLV 539

Query: 551 ALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDTIGNEHIACAVGTGPVDSAYKAV 610
           ALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVKL+D  G EH+AC+VGTGPVDSAYKAV
Sbjct: 540 ALVSDEVFQPDVVWKLLDLQVTCGTLGLSTATVKLIDADGREHVACSVGTGPVDSAYKAV 599

Query: 611 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKTYTSTNALTGEAVQRTFSGIGAGM 670
           DLI+KE  +LLEYSMNAVTEGIDAIATTRVLIR +K++ ST+ALTGE V RTFSG GAGM
Sbjct: 600 DLIIKESVSLLEYSMNAVTEGIDAIATTRVLIRVEKSHLSTHALTGELVHRTFSGTGAGM 631

Query: 671 DIVVSSVKAYIGALNKMLGFR 673
           DIVVSSVKAY+ ALNKMLGF+
Sbjct: 660 DIVVSSVKAYVSALNKMLGFK 631

BLAST of Lsi05G003000 vs. TrEMBL
Match: A0A0B0MU64_GOSAR (2-isopropylmalate synthase 2, chloroplastic-like protein OS=Gossypium arboreum GN=F383_10703 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 4.6e-210
Identity = 409/647 (63.21%), Postives = 469/647 (72.49%), Query Frame = 1

Query: 71  PKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIE 130
           P SS R PPYIPN IPDP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIE
Sbjct: 60  PPSSQRCPPYIPNHIPDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIE 119

Query: 131 AGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRI 190
           AGFPAASK+DFEAVK I+KEVGNAVD +GYVPVICGLSRCNE DI+TAWEAVKYAKRPRI
Sbjct: 120 AGFPAASKDDFEAVKTIAKEVGNAVDGNGYVPVICGLSRCNEKDIKTAWEAVKYAKRPRI 179

Query: 191 HTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGR---------- 250
           HTFIATS IH+++KLRK++E+V++IARNMVRFARSLGC DVEFSPEDAGR          
Sbjct: 180 HTFIATSAIHLEYKLRKSKEEVLDIARNMVRFARSLGCNDVEFSPEDAGRCMLVFAWGGL 239

Query: 251 ----------------SDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVADVKS 310
                           SDREFLY+ILGEVIKAGATT+NIPDTVG T+P EFG+L+AD+K+
Sbjct: 240 IISGALNDSYFAVAVVSDREFLYEILGEVIKAGATTLNIPDTVGITMPSEFGQLIADIKA 299

Query: 311 NTPGIENVIISTHCQNDLGLATANTVSGACAGARQVEV-----------------VMALQ 370
           NTPGIENVIISTHCQNDLGL+TANT++GACAGARQVEV                 VMAL+
Sbjct: 300 NTPGIENVIISTHCQNDLGLSTANTIAGACAGARQVEVTINGIGERAGNASMEEVVMALK 359

Query: 371 CRGEHVLGGLHTGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHE-SGIHQDGMLK 430
           CRGEHVLGGLHTGIN RH                     IV A+    E +G+H   +  
Sbjct: 360 CRGEHVLGGLHTGINTRH---------------------IVMASKMVEEYTGLH---VQP 419

Query: 431 HKGTYEIICPEDIGYERSSDAGIVLGKLRMTHENGAKVGTGS-DISSTRNTSFPRLEDLS 490
           HK    I+      +E    +GI        H++G     G+ +I S  +    R  +  
Sbjct: 420 HKA---IVGANAFAHE----SGI--------HQDGMLKHKGTYEIISPEDIGLERSNEAG 479

Query: 491 NVGHLIRRCIICEAFSVQAVPRELMWFFLGQWKCETSVAVLFGRHALKSRLLELGYELDD 550
            V   +R      +  +  +  ++             V  + GRHAL  RL ELGYELDD
Sbjct: 480 IVLGKLRFLTFTNSMVLCYLELDM----------NVGVGPMGGRHALSDRLKELGYELDD 539

Query: 551 ENLHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVK 610
             L N+FWRFK VAEQKKRVTDADL ALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVK
Sbjct: 540 VQLGNIFWRFKEVAEQKKRVTDADLVALVSDEVFQPDVVWKLLDLQVTCGTLGLSTATVK 599

Query: 611 LLDTIGNEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRG 670
           L+D  G EH+AC+VGTGPVDSAYKAVDLI+KE  +LLEYSMNAVTEGIDAIATTRVLIR 
Sbjct: 600 LIDADGREHVACSVGTGPVDSAYKAVDLIIKESVSLLEYSMNAVTEGIDAIATTRVLIRV 657

Query: 671 DKTYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFR 673
           +K++ ST+ALTGE V RTFSG GAGMDIVVSSVKAY+ ALNKMLGF+
Sbjct: 660 EKSHLSTHALTGELVHRTFSGTGAGMDIVVSSVKAYVSALNKMLGFK 657

BLAST of Lsi05G003000 vs. TrEMBL
Match: A0A0A0L7C8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 8.6e-209
Identity = 373/438 (85.16%), Postives = 398/438 (90.87%), Query Frame = 1

Query: 1   MAMPAALSRPNSHFLARQSNSQFPGRHRIYTRNLQVLKNPSRPLSFRTAAAD---NVSRI 60
           MA+PAALSRPN H LARQS+S FP  HRIY+RNLQVLKNPSR LSF+TAAA    NVS +
Sbjct: 1   MALPAALSRPN-HSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPV 60

Query: 61  YCSQTESIVSDSGPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDI 120
           Y   TESIVS+S PK+SIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLT KEK+DI
Sbjct: 61  YSCLTESIVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDI 120

Query: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRT 180
           ARQLAKLGVDIIEAGFPAASKEDFEAVKMI+KE+GNAVDEDGYVPVICGLSRCNE DIRT
Sbjct: 121 ARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRT 180

Query: 181 AWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPED 240
           AWEAVKYAKRPRIHTFIATSEIHM+HKLRKT+E+VIEIARNMVRFARSLGC+DVEFSPED
Sbjct: 181 AWEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPED 240

Query: 241 AGRSDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTH 300
           AGRSDREFLYQILGEVIKAGATT+NIPDTVGYT+P EFG+L+AD+KSNTPGIENVIIS+H
Sbjct: 241 AGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSH 300

Query: 301 CQNDLGLATANTVSGACAGARQV-----------------EVVMALQCRGEHVLGGLHTG 360
           CQNDLGLATANTV+GACAGARQV                 EVVMALQCRGEHVLGGLHTG
Sbjct: 301 CQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTG 360

Query: 361 INARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIG 419
           IN+RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQDGMLKHKGTYEI+ PEDIG
Sbjct: 361 INSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIG 420

BLAST of Lsi05G003000 vs. TrEMBL
Match: A0A0E0BNR8_9ORYZ (Uncharacterized protein OS=Oryza glumipatula PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 3.9e-185
Identity = 365/627 (58.21%), Postives = 437/627 (69.70%), Query Frame = 1

Query: 76  RRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPA 135
           RRP Y+PNRI DP+YVRVFDTTLRDGEQSPGA++T+ EK+ +ARQLA+LGVDIIEAGFPA
Sbjct: 73  RRPEYVPNRIDDPNYVRVFDTTLRDGEQSPGATMTSTEKLVVARQLARLGVDIIEAGFPA 132

Query: 136 ASKEDFEAVKMISKEVGNA-VDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFI 195
           +S +D +AV+ I+ EVGN  V EDG+VPVICGLSRCN+ DI  AWEAV++A+RPRIHTFI
Sbjct: 133 SSPDDLDAVRSIAIEVGNTPVGEDGHVPVICGLSRCNKRDIDAAWEAVRHARRPRIHTFI 192

Query: 196 ATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVI 255
           ATSEIHM+HKLRKT EQV+ IA+ MV +ARSLGC DVEFSPEDAGRS+REFLY IL EVI
Sbjct: 193 ATSEIHMQHKLRKTPEQVVAIAKEMVAYARSLGCPDVEFSPEDAGRSNREFLYHILEEVI 252

Query: 256 KAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGAC 315
           KAGATT+NIPDTVGYTLP EFG+L+AD+K+NTPGIEN IISTHCQNDLGLATANT++GA 
Sbjct: 253 KAGATTLNIPDTVGYTLPYEFGKLIADIKANTPGIENAIISTHCQNDLGLATANTLAGAH 312

Query: 316 AGARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYT 375
           AGARQ+                 EVVMA++CR E +LGGL+TGIN +HI ++SKMV+E++
Sbjct: 313 AGARQLEVTINGIGERAGNASLEEVVMAIKCRRE-LLGGLYTGINTQHITMSSKMVQEHS 372

Query: 376 GLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMT 435
           GL+VQPHKAIVGANAFAHESGIHQDGMLK+KGTYEII P+DIG  R+++ GIVLGKLR+ 
Sbjct: 373 GLHVQPHKAIVGANAFAHESGIHQDGMLKYKGTYEIISPDDIGLTRANEFGIVLGKLRL- 432

Query: 436 HENGAKVGTGSDISSTRNTSFPRLEDLSNVGHLIRRCIICEAFSVQAVPRELMWFFLGQW 495
                                       N+ HL  R  +      +   +E   FF    
Sbjct: 433 ----------------------------NIAHLTLRKALPYLLGYEITDKEFEDFFKRYK 492

Query: 496 KCETSVAVLFGRHALKSRLLELGYE--LDDENLH-NVFWRFKAVAEQKKRVTDADLRALV 555
           +              K R+ +   E  L DE     VFW                     
Sbjct: 493 EVAEK----------KKRVTDEDIEALLSDEIFQPKVFWS-------------------- 552

Query: 556 SDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDTIGNEHIACAVGTGPVDSAYKAVDLI 615
                       L D+Q TCGTLGLSTATVKL+   G+E IACAVGTGPVD+AYKAVD I
Sbjct: 553 ------------LADVQATCGTLGLSTATVKLIGPDGDEKIACAVGTGPVDAAYKAVDDI 612

Query: 616 VKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKTYTSTNALTGEAVQRTFSGIGAGMDIV 675
           ++ P  L EYSM +VTEGIDAIATTRV++ GD +  S +ALTG +  R FSG GA +DIV
Sbjct: 613 IQIPTVLREYSMTSVTEGIDAIATTRVVVTGDVS-DSKHALTGHSFNRAFSGSGAALDIV 625

Query: 676 VSSVKAYIGALNKMLGFRGVDVKVTQE 682
           VSSV+AY+ ALNKM  F G  +K + E
Sbjct: 673 VSSVRAYLSALNKMSSFVGA-IKASSE 625

BLAST of Lsi05G003000 vs. TrEMBL
Match: A0A0D2RKC1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G127500 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.3e-169
Identity = 297/365 (81.37%), Postives = 329/365 (90.14%), Query Frame = 1

Query: 71  PKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIE 130
           P SS RRPPYIPN IPDP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIE
Sbjct: 60  PPSSQRRPPYIPNHIPDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIE 119

Query: 131 AGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRI 190
           AGFPAASK+DFEAVK I+KEVGNAVD +GYVPVICGLSRCNE DI+TAWEAVKYAKRPRI
Sbjct: 120 AGFPAASKDDFEAVKTIAKEVGNAVDGNGYVPVICGLSRCNEKDIKTAWEAVKYAKRPRI 179

Query: 191 HTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQIL 250
           HTFIATS IH+++KLRK++E+V++IARNMVRF RSLGC DVEFSPEDAGRSDREFLY+IL
Sbjct: 180 HTFIATSAIHLEYKLRKSKEEVLDIARNMVRFTRSLGCNDVEFSPEDAGRSDREFLYEIL 239

Query: 251 GEVIKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTV 310
           GEVIKAGATT+NIPDTVG T+P EFG+L+AD+K+NTPGIENVIISTHCQNDLGL+TANT+
Sbjct: 240 GEVIKAGATTLNIPDTVGITMPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTI 299

Query: 311 SGACAGARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMV 370
           +GACAGARQV                 EVVMAL+CRGEHVLGGL+TGIN RHI + SKMV
Sbjct: 300 AGACAGARQVEVTINGIGERAGNASMEEVVMALKCRGEHVLGGLYTGINTRHIVMASKMV 359

Query: 371 EEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGK 419
           EEYTGL+VQPHKAIVGANAFAHESGIHQDGMLKHKGTYEII PEDIG ERS++AGIVLGK
Sbjct: 360 EEYTGLHVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNEAGIVLGK 419

BLAST of Lsi05G003000 vs. TAIR10
Match: AT1G74040.1 (AT1G74040.1 2-isopropylmalate synthase 1)

HSP 1 Score: 586.6 bits (1511), Expect = 1.9e-167
Identity = 292/394 (74.11%), Postives = 335/394 (85.03%), Query Frame = 1

Query: 42  RPLSFRTAAADNVSRIYCSQTESIVSDSGPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDG 101
           R  + RT +     +   S + S  S   P +  RRP YIPNRI DP+YVR+FDTTLRDG
Sbjct: 38  RSQNHRTISLTTAGKFRVSYSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDG 97

Query: 102 EQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDEDGYV 161
           EQSPGA+LT+KEK+DIARQLAKLGVDIIEAGFPAASK+DFEAVK I++ VGN VDE+GYV
Sbjct: 98  EQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYV 157

Query: 162 PVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARNMVR 221
           PVICGLSRCN+ DI TAWEAVKYAKRPRIHTFIATS+IH+K+KL+K++E+VIEIARNMVR
Sbjct: 158 PVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVR 217

Query: 222 FARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVAD 281
           FARSLGCEDVEFSPEDAGRS+RE+LY+ILGEVIKAGATT+NIPDTVG TLP EFG+L+AD
Sbjct: 218 FARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIAD 277

Query: 282 VKSNTPGIENVIISTHCQNDLGLATANTVSGACAGARQV-----------------EVVM 341
           +K+NTPGI+NVIISTHCQNDLGL+TANT+SGA +GARQV                 EVVM
Sbjct: 278 IKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVM 337

Query: 342 ALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGM 401
           A++CRG+HVLGGL TGI+ RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQDGM
Sbjct: 338 AIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGM 397

Query: 402 LKHKGTYEIICPEDIGYERSSDAGIVLGKLRMTH 419
           LKHKGTYEI+ PE+IG ERS+DAGIVLGKL   H
Sbjct: 398 LKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRH 431

BLAST of Lsi05G003000 vs. TAIR10
Match: AT1G18500.1 (AT1G18500.1 methylthioalkylmalate synthase-like 4)

HSP 1 Score: 577.0 bits (1486), Expect = 1.5e-164
Identity = 282/359 (78.55%), Postives = 320/359 (89.14%), Query Frame = 1

Query: 77  RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAA 136
           RP YIPNRI DP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 137 SKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 196
           SK+DFEAVK I++ VGN VDE+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 197 SEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKA 256
           S+IH+++KL+KT+ +VIEIAR+MVRFARSLGCEDVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 257 GATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGACAG 316
           GATT+NIPDTVG TLP EFG+L+ D+K+NTPGIENV+ISTHCQNDLGL+TANT+SGA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 317 ARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGL 376
           ARQ+                 EVVMA++CRG+HVLGGL TGI+ RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 377 NVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMTH 419
             QPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPE+IG ERS+DAGIVLGKL   H
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRH 433

BLAST of Lsi05G003000 vs. TAIR10
Match: AT5G23020.1 (AT5G23020.1 2-isopropylmalate synthase 2)

HSP 1 Score: 451.8 bits (1161), Expect = 7.4e-127
Identity = 225/390 (57.69%), Postives = 295/390 (75.64%), Query Frame = 1

Query: 76  RRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPA 135
           RRP YIPN++P  +YVRV DTTLRDGEQSPGA+LT  +K++IARQLAKL VDI+E GFP 
Sbjct: 70  RRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPV 129

Query: 136 ASKEDFEAVKMISKEVGNAVDED-GYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFI 195
           +S+E+FEA+K I+K VGN VDE+ GYVPVICG++RC + DI   WEA+KYAKRPR+  F 
Sbjct: 130 SSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFT 189

Query: 196 ATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVI 255
           +TSEIHMK+KL+KT+E+VIE+A N V++A+SLG +D++F  ED GR++++F+ +ILGE I
Sbjct: 190 STSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGESI 249

Query: 256 KAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGAC 315
           KAGATT+   DTVG  +P EFGELVA V  NTPG ++++ + HC NDLG+ATANT+SG C
Sbjct: 250 KAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISGIC 309

Query: 316 AGARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYT 375
           AGARQV                 EVVMAL+CRGE ++ G++T I++R I  TSKMV+E+T
Sbjct: 310 AGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHT 369

Query: 376 GLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMT 435
           G+ VQPHK IVG N F HESGIHQDG+LK++ TYEI+ PED+G  +S ++GIVLGKL   
Sbjct: 370 GMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGR 429

Query: 436 HENGAKV-GTGSDISSTR-NTSFPRLEDLS 446
           H    ++   G +IS  + N  F R  +L+
Sbjct: 430 HAVKDRLKELGYEISDEKFNDIFSRYRELT 459

BLAST of Lsi05G003000 vs. TAIR10
Match: AT5G23010.1 (AT5G23010.1 methylthioalkylmalate synthase 1)

HSP 1 Score: 444.9 bits (1143), Expect = 9.0e-125
Identity = 218/361 (60.39%), Postives = 277/361 (76.73%), Query Frame = 1

Query: 76  RRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPA 135
           R P YIPN++PD +YVRVFDTTLRDGEQSPG SLT  +K++IARQLAKL VDI+E GFP 
Sbjct: 70  RWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQKLEIARQLAKLRVDIMEVGFPG 129

Query: 136 ASKEDFEAVKMISKEVGNAVDED-GYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFI 195
           +S+E+ E +K I+K VGN VDE+ GYVPVIC ++RC   DI   WEA+KYAKRPRI  F 
Sbjct: 130 SSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKHRDIEATWEALKYAKRPRILVFT 189

Query: 196 ATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVI 255
           +TS+IHMK+KL+KT+E+VIE+A + +RFA+SLG  D++F  ED GRSD++FL +ILGE I
Sbjct: 190 STSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQFGCEDGGRSDKDFLCKILGEAI 249

Query: 256 KAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGAC 315
           KAG T + I DTVG  +P E+GELV  +K+NTPGI++V+++ HC NDLGLATAN+++G  
Sbjct: 250 KAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDVVVAVHCHNDLGLATANSIAGIR 309

Query: 316 AGARQV-----------------EVVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYT 375
           AGARQV                 EVVMAL+CRG +V+ G++T I+ R I  TSKMV+EYT
Sbjct: 310 AGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVINGVYTKIDTRQIMATSKMVQEYT 369

Query: 376 GLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMT 419
           GL VQ HK IVGAN F HESGIHQDG+LK++ TYEI+ PEDIG  +S ++G+VLGKL   
Sbjct: 370 GLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDIGIVKSQNSGLVLGKLSGR 429

BLAST of Lsi05G003000 vs. NCBI nr
Match: gi|6714306|gb|AAF26002.1|AC013354_21 (F15H18.3 [Arabidopsis thaliana])

HSP 1 Score: 815.1 bits (2104), Expect = 9.4e-233
Identity = 431/629 (68.52%), Postives = 492/629 (78.22%), Query Frame = 1

Query: 77  RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAA 136
           RP YIPNRI DP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 137 SKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 196
           SK+DFEAVK I++ VGN VDE+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 197 SEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKA 256
           S+IH+++KL+KT+ +VIEIAR+MVRFARSLGCEDVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 257 GATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTVSGACAG 316
           GATT+NIPDTVG TLP EFG+L+ D+K+NTPGIENV+ISTHCQNDLGL+TANT+SGA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 317 ARQVEV-----------------VMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGL 376
           ARQ+EV                 VMA++CRG+HVLGGL TGI+ RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 377 NVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMTHE 436
             QPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPE+IG ER                
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLER---------------S 434

Query: 437 NGAKVGTGSDISSTRNTSFPRLEDL-SNVGHLIRRCIICEAFSVQAVPRELMWFFLGQWK 496
           N A +  G    S R+    RL ++ SN+ H    CI                 F+G   
Sbjct: 435 NDAGIVLGK--LSGRHALKDRLTEVHSNLTHT-NTCIA---------------IFVG--- 494

Query: 497 CETSVAVLFGRHALKSRLLELGYELDDENLHNVFWRFKAVAEQKKRVTDADLRALVSDEV 556
            +T+V + F          +LGY+LDDE L  +FWRFK VAEQKKRVTDAD+ ALVSDEV
Sbjct: 495 -DTNVLLSFVH-------FQLGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEV 554

Query: 557 FQPTVLWKLLDLQVTCGTLGLSTATVKLLDTIGNEHIACAVGTGPVDSAYKAVDLIVK-- 616
           FQP  +WKLLD+Q+TCGTLGLSTATVKL D  G EH+AC++GTGPVDSAYKAVDLIVK  
Sbjct: 555 FQPEAVWKLLDIQITCGTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKVL 614

Query: 617 -------------EPATLLEYSMNAVTEGIDAIATTRVLIRGDKTYTSTNALTGEAVQRT 673
                        EPATLLEYSMNAVTEGIDAIATTRVLIRG   Y+STNA+TGE VQRT
Sbjct: 615 FIPLRLSSTNNSPEPATLLEYSMNAVTEGIDAIATTRVLIRGSNKYSSTNAITGEEVQRT 659

BLAST of Lsi05G003000 vs. NCBI nr
Match: gi|922552609|ref|XP_013604296.1| (PREDICTED: 2-isopropylmalate synthase 1, chloroplastic-like isoform X1 [Brassica oleracea var. oleracea])

HSP 1 Score: 777.7 bits (2007), Expect = 1.7e-221
Identity = 410/594 (69.02%), Postives = 469/594 (78.96%), Query Frame = 1

Query: 99  RDGEQSPGASLTAKEKIDIARQLAKLGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDED 158
           RDGEQSPGA+LT+KEK+DIARQLAKLGVDIIEAGFPAASK+DFEAVK IS+ VGNAVDE+
Sbjct: 65  RDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTISETVGNAVDEN 124

Query: 159 GYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARN 218
           GYVPVICGLSRCN+ DI+TAWEAV+YAKRPRIHTFIATS+IH+++KL+K+R++VIEIAR+
Sbjct: 125 GYVPVICGLSRCNKKDIQTAWEAVRYAKRPRIHTFIATSDIHLEYKLKKSRQEVIEIARS 184

Query: 219 MVRFARSLGCEDVEFSPEDAGRSDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGEL 278
           MVRFARSLGC+DVEFSPEDAGRS+REFLY+ILGEVIKAGATT+NIPDTVG TLP EFG+L
Sbjct: 185 MVRFARSLGCDDVEFSPEDAGRSEREFLYEILGEVIKAGATTLNIPDTVGITLPSEFGQL 244

Query: 279 VADVKSNTPGIENVIISTHCQNDLGLATANTVSGACAGARQV-----------------E 338
           +AD+K+NTPGIENV+ISTHCQNDLGL+TANT+SGA AGARQV                 E
Sbjct: 245 IADLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQVEVTINGIGERAGNASLEE 304

Query: 339 VVMALQCRGEHVLGGLHTGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQ 398
           VVMA++CRG+HVLGGL TGI+ RHI +TSKMVE+YTG+  QPHKAIVGANAFAHESGIHQ
Sbjct: 305 VVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEDYTGMQTQPHKAIVGANAFAHESGIHQ 364

Query: 399 DGMLKHKGTYEIICPEDIGYERSSDAGIVLGKLRMTH---ENGAKVGTGSDISSTRNTSF 458
           DGMLKHKGTYEIICPE+IG ERS+DAGIVLGKL   H   +   +V      + TR    
Sbjct: 365 DGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTEVHINLIQTHTRYYHL 424

Query: 459 PRLEDLSNVGHLIRRCIICEAFSVQAVPRELMWFFLGQWKCETSVAVLFGRHALKSRLLE 518
               D + + H                    + F LG    +  ++ +F R         
Sbjct: 425 LWEGDTNVLSH-------------------FLLFQLGYVLDDEQLSTIFWRF-------- 484

Query: 519 LGYELDDENLHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLG 578
                            K+VAEQKKRVTDAD+ ALVSDEVFQP  LWKLLD+Q+TCGTLG
Sbjct: 485 -----------------KSVAEQKKRVTDADIIALVSDEVFQPEALWKLLDIQITCGTLG 544

Query: 579 LSTATVKLLDTIGNEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIAT 638
           LSTATVKL D  G EH AC++GTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIAT
Sbjct: 545 LSTATVKLADADGKEHCACSMGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIAT 604

Query: 639 TRVLIRGDKTYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFR 673
           TRVL+RG+ TYTSTNA+TGE V RTFSG GAGMDIVVSSVKAY+GALNKML F+
Sbjct: 605 TRVLVRGNNTYTSTNAITGEEVLRTFSGTGAGMDIVVSSVKAYVGALNKMLDFK 614

BLAST of Lsi05G003000 vs. NCBI nr
Match: gi|728808800|gb|KHF98529.1| (2-isopropylmalate synthase 2, chloroplastic -like protein [Gossypium arboreum])

HSP 1 Score: 753.4 bits (1944), Expect = 3.4e-214
Identity = 409/621 (65.86%), Postives = 469/621 (75.52%), Query Frame = 1

Query: 71  PKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIE 130
           P SS R PPYIPN IPDP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIE
Sbjct: 60  PPSSQRCPPYIPNHIPDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIE 119

Query: 131 AGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRI 190
           AGFPAASK+DFEAVK I+KEVGNAVD +GYVPVICGLSRCNE DI+TAWEAVKYAKRPRI
Sbjct: 120 AGFPAASKDDFEAVKTIAKEVGNAVDGNGYVPVICGLSRCNEKDIKTAWEAVKYAKRPRI 179

Query: 191 HTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGRSDREFLYQIL 250
           HTFIATS IH+++KLRK++E+V++IARNMVRFARSLGC DVEFSPEDAGRSDREFLY+IL
Sbjct: 180 HTFIATSAIHLEYKLRKSKEEVLDIARNMVRFARSLGCNDVEFSPEDAGRSDREFLYEIL 239

Query: 251 GEVIKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIISTHCQNDLGLATANTV 310
           GEVIKAGATT+NIPDTVG T+P EFG+L+AD+K+NTPGIENVIISTHCQNDLGL+TANT+
Sbjct: 240 GEVIKAGATTLNIPDTVGITMPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTI 299

Query: 311 SGACAGARQVEV-----------------VMALQCRGEHVLGGLHTGINARHIFLTSKMV 370
           +GACAGARQVEV                 VMAL+CRGEHVLGGLHTGIN RH        
Sbjct: 300 AGACAGARQVEVTINGIGERAGNASMEEVVMALKCRGEHVLGGLHTGINTRH-------- 359

Query: 371 EEYTGLNVQPHKAIVGANAFAHE-SGIHQDGMLKHKGTYEIICPEDIGYERSSDAGIVLG 430
                        IV A+    E +G+H   +  HK    I+      +E    +GI   
Sbjct: 360 -------------IVMASKMVEEYTGLH---VQPHKA---IVGANAFAHE----SGI--- 419

Query: 431 KLRMTHENGAKVGTGS-DISSTRNTSFPRLEDLSNVGHLIRRCIICEAFSVQAVPRELMW 490
                H++G     G+ +I S  +    R  +   V   +R      +  +  +  ++  
Sbjct: 420 -----HQDGMLKHKGTYEIISPEDIGLERSNEAGIVLGKLRFLTFTNSMVLCYLELDM-- 479

Query: 491 FFLGQWKCETSVAVLFGRHALKSRLLELGYELDDENLHNVFWRFKAVAEQKKRVTDADLR 550
                      V  + GRHAL  RL ELGYELDD  L N+FWRFK VAEQKKRVTDADL 
Sbjct: 480 --------NVGVGPMGGRHALSDRLKELGYELDDVQLGNIFWRFKEVAEQKKRVTDADLV 539

Query: 551 ALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDTIGNEHIACAVGTGPVDSAYKAV 610
           ALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVKL+D  G EH+AC+VGTGPVDSAYKAV
Sbjct: 540 ALVSDEVFQPDVVWKLLDLQVTCGTLGLSTATVKLIDADGREHVACSVGTGPVDSAYKAV 599

Query: 611 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKTYTSTNALTGEAVQRTFSGIGAGM 670
           DLI+KE  +LLEYSMNAVTEGIDAIATTRVLIR +K++ ST+ALTGE V RTFSG GAGM
Sbjct: 600 DLIIKESVSLLEYSMNAVTEGIDAIATTRVLIRVEKSHLSTHALTGELVHRTFSGTGAGM 631

Query: 671 DIVVSSVKAYIGALNKMLGFR 673
           DIVVSSVKAY+ ALNKMLGF+
Sbjct: 660 DIVVSSVKAYVSALNKMLGFK 631

BLAST of Lsi05G003000 vs. NCBI nr
Match: gi|659114493|ref|XP_008457079.1| (PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 749.6 bits (1934), Expect = 4.9e-213
Identity = 379/440 (86.14%), Postives = 400/440 (90.91%), Query Frame = 1

Query: 1   MAMPAALSRPNSHFLARQSNSQFPGRHRIYTRNLQVLKNPSRPLSFRTAAA-----DNVS 60
           MA+PAALSRPN HFLARQS+S FP RH IYTRN QVLKNPSR LSF+TAAA     DNVS
Sbjct: 1   MALPAALSRPN-HFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAAAAPDNVS 60

Query: 61  RIYCSQTESIVSDSGPKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKI 120
           RIYC  TES+VS+SGPKSS RRPPYIPNRIPDPSYVR+FDTTLRDGEQSPGASLT KEK+
Sbjct: 61  RIYCCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKL 120

Query: 121 DIARQLAKLGVDIIEAGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDI 180
           DIARQLAKLGVDIIEAGFPAASKEDFEAVKMI+KE+G+AVDEDGYVPVICGLSRCNE DI
Sbjct: 121 DIARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDI 180

Query: 181 RTAWEAVKYAKRPRIHTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSP 240
           RTAWEAVKYAKRPRIHTFIATSEIHM+HKLRKTREQVIEIARNMVRFARSLGC+DVEFSP
Sbjct: 181 RTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSP 240

Query: 241 EDAGRSDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVADVKSNTPGIENVIIS 300
           EDAGRSDREFLYQILGEVIKAGATT+NIPDTVGYT+P EFG+L+AD+KSNTPGIENVIIS
Sbjct: 241 EDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIS 300

Query: 301 THCQNDLGLATANTVSGACAGARQV-----------------EVVMALQCRGEHVLGGLH 360
           THCQNDLGLATANTV+GACAGARQV                 EVVMALQCRGEHVLGGLH
Sbjct: 301 THCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLH 360

Query: 361 TGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPED 419
           TGIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQDGMLKHKGTYEI+ PED
Sbjct: 361 TGINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPED 420

BLAST of Lsi05G003000 vs. NCBI nr
Match: gi|728824163|gb|KHG05648.1| (2-isopropylmalate synthase 2, chloroplastic -like protein [Gossypium arboreum])

HSP 1 Score: 739.2 bits (1907), Expect = 6.6e-210
Identity = 409/647 (63.21%), Postives = 469/647 (72.49%), Query Frame = 1

Query: 71  PKSSIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTAKEKIDIARQLAKLGVDIIE 130
           P SS R PPYIPN IPDP+YVRVFDTTLRDGEQSPGA+LT+KEK+DIARQLAKLGVDIIE
Sbjct: 60  PPSSQRCPPYIPNHIPDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIE 119

Query: 131 AGFPAASKEDFEAVKMISKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRI 190
           AGFPAASK+DFEAVK I+KEVGNAVD +GYVPVICGLSRCNE DI+TAWEAVKYAKRPRI
Sbjct: 120 AGFPAASKDDFEAVKTIAKEVGNAVDGNGYVPVICGLSRCNEKDIKTAWEAVKYAKRPRI 179

Query: 191 HTFIATSEIHMKHKLRKTREQVIEIARNMVRFARSLGCEDVEFSPEDAGR---------- 250
           HTFIATS IH+++KLRK++E+V++IARNMVRFARSLGC DVEFSPEDAGR          
Sbjct: 180 HTFIATSAIHLEYKLRKSKEEVLDIARNMVRFARSLGCNDVEFSPEDAGRCMLVFAWGGL 239

Query: 251 ----------------SDREFLYQILGEVIKAGATTINIPDTVGYTLPVEFGELVADVKS 310
                           SDREFLY+ILGEVIKAGATT+NIPDTVG T+P EFG+L+AD+K+
Sbjct: 240 IISGALNDSYFAVAVVSDREFLYEILGEVIKAGATTLNIPDTVGITMPSEFGQLIADIKA 299

Query: 311 NTPGIENVIISTHCQNDLGLATANTVSGACAGARQVEV-----------------VMALQ 370
           NTPGIENVIISTHCQNDLGL+TANT++GACAGARQVEV                 VMAL+
Sbjct: 300 NTPGIENVIISTHCQNDLGLSTANTIAGACAGARQVEVTINGIGERAGNASMEEVVMALK 359

Query: 371 CRGEHVLGGLHTGINARHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHE-SGIHQDGMLK 430
           CRGEHVLGGLHTGIN RH                     IV A+    E +G+H   +  
Sbjct: 360 CRGEHVLGGLHTGINTRH---------------------IVMASKMVEEYTGLH---VQP 419

Query: 431 HKGTYEIICPEDIGYERSSDAGIVLGKLRMTHENGAKVGTGS-DISSTRNTSFPRLEDLS 490
           HK    I+      +E    +GI        H++G     G+ +I S  +    R  +  
Sbjct: 420 HKA---IVGANAFAHE----SGI--------HQDGMLKHKGTYEIISPEDIGLERSNEAG 479

Query: 491 NVGHLIRRCIICEAFSVQAVPRELMWFFLGQWKCETSVAVLFGRHALKSRLLELGYELDD 550
            V   +R      +  +  +  ++             V  + GRHAL  RL ELGYELDD
Sbjct: 480 IVLGKLRFLTFTNSMVLCYLELDM----------NVGVGPMGGRHALSDRLKELGYELDD 539

Query: 551 ENLHNVFWRFKAVAEQKKRVTDADLRALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVK 610
             L N+FWRFK VAEQKKRVTDADL ALVSDEVFQP V+WKLLDLQVTCGTLGLSTATVK
Sbjct: 540 VQLGNIFWRFKEVAEQKKRVTDADLVALVSDEVFQPDVVWKLLDLQVTCGTLGLSTATVK 599

Query: 611 LLDTIGNEHIACAVGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRG 670
           L+D  G EH+AC+VGTGPVDSAYKAVDLI+KE  +LLEYSMNAVTEGIDAIATTRVLIR 
Sbjct: 600 LIDADGREHVACSVGTGPVDSAYKAVDLIIKESVSLLEYSMNAVTEGIDAIATTRVLIRV 657

Query: 671 DKTYTSTNALTGEAVQRTFSGIGAGMDIVVSSVKAYIGALNKMLGFR 673
           +K++ ST+ALTGE V RTFSG GAGMDIVVSSVKAY+ ALNKMLGF+
Sbjct: 660 EKSHLSTHALTGELVHRTFSGTGAGMDIVVSSVKAYVSALNKMLGFK 657