Carg07441 (gene) Silver-seed gourd

NameCarg07441
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description2-isopropylmalate synthase 2
LocationCucurbita_argyrosperma_scaffold_019 : 837670 .. 845439 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTGCCGGTAACTTTGTCGAGTCCGAATCACTTTCTTGTTCGGCATTCGAACTCTCAGTTTCCTTCCTGTCATCGGCGTTACAATCGGAATTTGCAGGCTCTTAAGAATTCTTCACGGTCGTTGTCCTTTAGAACGGTGGCGGACAATGTGTCTCGCATTTACTGCAGTCACACTGAAAGCGTAGTTTCGAATTCCAAATCGAATTCATCCATTAAACGTCCGCCGTATATTCCTAATCGCATCCCTGACTCTTCCTATGTTCGGCTATTTGATACCACTCTCCGCGATGGCGAGCAGTCTCCAGGTGCCTCCCTCACGGCTAAAGAGAAGCTTGATATTGCTCGTCAGCTCGTCAAGCTTGGTGTTGACATAATTGAGGCTGGATTTCCTGCTGCTTCTAAGGAAGACTTTGAGGCCGTCAAGATGATTGCCGCAGACGTTGGCAATGCTGTCAATGAGGACGGATATGTGCCTGTTATTTGTGGTCTTTCGAGGTGTAATGAGAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCACGGATACATACGTTTATAGCAACAAGCGAAATTCATATGGAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGAGATTGCTCGAAATATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTTAGCCCCGAGGATGCTGGGAGGTTCGATCTTGATCTCAATTTACATGCATTTTCTGATTTTTGTTTCAATGATTGATCCTGAGAGTAAGTTGACTTCGCTTGATAATTTCAAAACCTCTCCATCATAATGGATGTTTGGATTAAGTTGTTAATAAGTTCAAATTTATGAAGCTTGAAGTTGTGAAGTGGGAACTTATGTAATTTGTTATTGTATCCCGTAAAAGAATAGGATCTAAGATATGTAAATCTTTGTATTTTGTTTGTTTTCCTGATGCTTGATTTATGTAATTTGGATTTGCGTAGCTAATGGTTTGCAATTTTTTATTAGCAGAATCATATGCTAGTAGCCTTCTTACTGTTCATTGTGTAATCACTGACTGATGTGTCTATCCCCATCCTTGTCTTGACGAGAAAAGATCCTTCATAGGGACCCTTCTCTATGGGGGATTTCGACATCCTTAGCCATCGTTGCTTAAAATAAAATTGCAGTTCTTTTAAGTTAATAAAATAGTTTTCTTTTTTTATGTTGTGGTGTTCTATCAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGCGAAGTGATAAAGGCTGGTGCAACTACTCTCAACATACCAGATACTGTGGGTTATACAATGCCAGTTGAATTTGGCAACTTGATTGCTGATATAAAAGCTAATACTCCTGGCATTGATAATGTTATCATCTCAACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGTAAGTAATGGCATTTTCCTCCCTTTTAATCCTATTTATGACGTGTTTGGGAATGTGGCCAAAATCACCTTTTTCATGCCCAAAAACACTAAACAGGGGATGAAATTCCAAAATCACTCCTAAACATGTCTTTATTTATTGAATTAAATTAGGTCTGTAATTTTGGTAAGTATCCGAATATAATTGGAAAGTTACAAGGATTATGAATAACATACCATAACTCTAAGCATGATATAGAGAATTAACTTCGTAATCAATACCCAAATTATGATTCTTCTCATCTCTTTGCAGGGGGCATGTGCAGGCGCAAGGCAACTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCATCGCTAGAGGAGGCAAGTCAATTTTTTTTTTTTCTGTGTTTATTTCATATTTTTATTTTGTGAAGAGTGACGATGACTTGGAATGCAACCTGGCTTAATTGGTAATCAGTATGTCGTACTTACTTCATACTATGTTTTTTATTTACAAAAGAGGTTAAATTTTAAATTCTGAGAAATTAAGAGTGTTTATATATTTCATTTTACATGCGACTGTATGCATGTTTCTGTTGTATTGTTCTAAGAACATCCGTTTGTGCAGACATCAAAACATTCATATTTCGTGGGTACACTTCTTAAATGGCATGTATGAATGTACACAATTTTCATTTTTTATACCATATTTTTAAATCTTCAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCGTACTGGAATTAACTCGAGACATATCTTTTTGACTAGCAAGATGGTAATTACTTTTCTTACAAAAAATGCTTCTTGAAAAAAATTCCTTAAGTTATTCTTTTTATTATTCTTTTGGATGCTAGACAGTTCTGTTTCGCTTCTGGTTCCTCTTTAAAAGATTTGGAAAGAGTCGAGGCAATTTTTTTGAGGATAGAGATAGGAGCATTTATTTCATTTTTTAGGGGTAGGGCTGGTACTTTGAGAGATGTTTAATGTTACTAATTAGGTATGAATGTCCTAACTTCTATTTATTTTATTTCTCTTATGACTACTGTTTCCTTTTAAAATCTGCTGGTTGGACTTTTTCCTCCTTTGAGGCGAAGTATATTTGGGAATTTGGATTAAGGACAGATTTGGTAGCTCTCTCTTTCCCCCAGTTCTCTTTTTTGTTTTTCTATTAAATATCTCTATTTGTTATCAATAAAGGCTACTTATTGACTAACTCATTCCTTAGGTAGAAGAGTACACTGGTTTGAATGTACAACCACACAAGGCAATTGTGGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGTTTGTCACGTTCAATTTTGATTTTTGCTATTCGCAGCTAACTATTTATGACACATCCTCATATTTATCTTTCAAAACCTATCGTGATTTGAAGGCTTCCCTATAAGCAGACTATTACAAAATTCTAGCACCTAGGGGTGTATATCCAACCCGACAACCCGGACCAACCTAACCCGAATTATAGGGGTTGGGTTGGTTTGGGTTGCTTTTTTTGAACTCTGAGTAATTCGGCTCGGTTTCAGGTTCTTAGGATAAAAAACTCGGGTTGACCCGAAATCGAACAAAACTTATAAATTACATTGAGAATTCTGGCATATGTAAAGCAATCCGAGTTTATTAGAATACTCTCGTTTGCTCAAGCCAAATTCATCTTTTTATTATTTTGTTTGTTTCCTTTATTTATTTATTTATTTTTATTTTTTTGTTTCTTTTATATTTTTTTATTACTATTTTTTTATTATTATTTTTTTGTTTCTTTTATATTTTTTTATTACTATTTTTTTATTATTATTTTATTTAATTTCTTTTATATGCTTTAAAATTCTCTACTAAATATATAAAAATACTTCATTATTTCATATTATTTTTAGATGCATTATTTATTATTTTTTAATTTTTGTTGTTTCTTTTATATTATTTTATTTAATTTCTTTTATATATTTTAAAATTCTCTTCTAAATATATAAAAATATCTCAATATTTCATACTATTTTTAAACACATTATTATAATGTTAACTAATCATTTTTATTGTTTTTTAATTTCCTAATCTATCCTTATATTTTCTTTGTAACCAATTATTGTCTAACTATGTTAAGAACTTACAATTTTTCTTTATTTGATGATTTTTTATTTTATTTTATTATTATTTTTTTTTATAATAATTATAGATTGATTTGGGTTTGTTGGTAGACTTTTTAATATTTTTTTTTATCTATTATTATATCAAATAATTATGAAGGGTGAATAAATAAAAAAATTTTAAAAAATTAACAACCGAACAACCCAACCCGAAAAATTAACAACCGAACAACACAACCCGAAACTTTTCGGGTTGGGTTGGCTTACCAATTCAACCAACCCGAACTTTTGGGATCGGTAAAAAAAAACCTTTCAACCCAATTCAACCTGGACCATGTACACCCCTACCACTGGTATTTATTGTTTTCAACACATTTATGGCAAACAATTACTCATTTTTAACTTTAGTTCCCCTTTTCTTGCGATTTAACTTTTGGATAATGAAATTCAGAAAACAAAGTTAGGAAGGATGGTAATGGAAGATGAGTGTTTCCTACGAGGTGTTGAAGACTATACTGAATTCTCCCCAATTTGGTAGATTGGACAAGAAGCGTGAAAAACCTTATCTTAAGCACTTCAAGAGGAAGGAAGAAAATTGTTAAACAGCTGCTAAGTTCTCTTACAAATATCGAGAACTCTTTTAAACTATTTTAATGTATAGTCTTGCTTTAAAGCTCATTATGAAAATGTTGATTGGCTCTTATTTCTATGTTTTGAGTAATTATTATAATTATTATTATTTGTGTGAGACAAGCGGTCGGCTGTTTGCCCTGCTTTGTTAGGATCCAATTGTATCTTGAACATTGCTTTTGTGGTGCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTCAGGTACACCATGTTGCTTTATGGAAAATGACAATATTGCCTTTCTCTCGATAAAATTTCCCCTTCTCCTTGCCTCCTCTTCCCCATCTCTTTATGTGACTCTACGACATTTGATTTTCGAGGTAACTCATACAATATTAAATCCTCCTAGGGATTACAATGACTTGATGAACCCATTCCCATTTTCCGTTGAAAGTCAGATTAAGTTCTAAAATATTTAGAATAGACATGGAAAAATTGTTCTCTTTAGATCTAAATCTTCTTATAGCTGGGTTCAACAATGCAACAGTACGAAGGAACTAAATAGTTTTTCAGGGACAGGAGAAATGACAAACATCTGTTGTGGTTCTGTTGTAGTGCTGCTCAAAATCATTTTTTTACAATATTAACAGGATTGACTTTATATGTTTATTTCACTTTCTGTGTCTTGTTCAAAGTATACTATCCCAATAACGTGTTCATTATGACTACATTTACAGTGGACGCCATGCCCTTAAAAGTCGACTTCTAGAGGTAAATTTATATCTCCAAGCTCAATGTTTAGGTAATATTTCCATTTTATAATAGGCTAATGTTATTTGTCTTTTCAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACGTATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGGTAACTCAAAATTTTGTCCCTCTCTTTTGCTGAGCTATTCCCTATCCTGCTTCCTACACATAGTAGAGTAGCAAATACCAATTGGGATAATTCAGCCTGTTTCAAGAACATAAATAACTACTCATCAAGGAAGTTTTTCATTTAACCTGTTCTAAACTTTTTGCCATAATTTATCTTCAACATTCCAGAGAGTTACAGATATGGATCTTCGAGCATTGGTATCTGATGAGGTTTTACAACCAACTGTTCTATGGAAGCTGCTAGATTTGCAGGTTATCTAAATATTGTTATAGATCGTCAGATTCGGAAAGTCGTTTGGGCCTATTTTTAGATACACATAGCATCATTTGACAATAGCACCTCAAATGCAGATCACTTGTGGAACTCTCGGTCTTTCCACTGCTACTGTTAAACTCCTTGATGCCGATGGGAAAGAGCACGTTGCTTGTGCTGTTGGAACGGGCCCTGTAGATTCAGCTTACAAGGCTGTTGATCTAATTATTAAGGTTTGTGATAATAAGAGAAACCGCTGTTAAACATTCTGCAGTTTTAATTTACATCTAGAATACATCTTCAGGATGATTTCCTAGTAAGGTAAAATTAGGATTTTCCAGATAATTGGCTTTTACACCAAAGTGTTAGGGATTGAATTCAAACCATGGAGGCTACCTATCTAGGATTTAATATCCTACAAATTATCTTGGCAACCAAATTTTGTGGCGTTGTATGGTTGTTTCTTGAGAATTGTTGAAATGCATGGGAGATGGCTCAGTCAAAAAAACAATCAAAGAAATTATTTTCCTCTGAAGAGTTTAAAATGCAGGCTTGTTGGATTCGTTGTGATGCCGTGACATTTGGTGAATATAGCACAAATGGCATGATCGACAACCAGGCTTTCAAAACCTTGCATGCTTTGATTCGTGTCCTTGAATGAATGCGACGTTACAGAGTTTCCATTTTTCTGAATGCAGGAACCAGTAACACTGCTCGATTATTCAATGAATTCTGTCACCGAAGGGATCGATGCAATTGCCACTACTCGTGTTGTAATAAGAGGGGACAACAGTTACTTATCTACTAATGCATCGACTGGAGAAGAAGTTCAACGAACATTTAGGTATACGTTTTCTTCCCTATCGTTTAAAGTAATTAAACTAAGCTATTATCTTCTAAACTTCTTCAATTTTGCCCATCTCGTTGTCTGTTGGTACAAATTTTCTAAGTTTGTCTCCACATTCCTATTTAAGTTGGCAAAAATTCCAAATTTTCTTAGGTGATCGTTCCTTTTCTGATTTTTGCCAATGACTATATAGCATTCCTGGAAGAACATAAAAGGGTATAATTAATAAAAATTTAAAACACAAGGGACTAAATTGAAAGAACACTCCAACATTAGTGACTAAACTATATATTTTAACAATTTTTAAATACAATCATATTCAAGTACTTTTGCCTCTATGTTATTGGATCTAGGCATCCCTATGTTCAACTTGAGAAGACTAGGATGGCCATTGGTATCGTGAAAAATAAAGGGATTTAGAAGGAATGAATTCAAACTAGGGTAGTAACCTATATGGAGAATTTAAACGGTTTGTCTTGCAACTAATTGTACTTGTAAGCTTGTTTCCAGCCTTTCTCTCCCCCTAACCTAACAGGCGAAGTTCACAACTTTGAGCTCTAGATTTCGATAGGAACAACCACATCACTACTTACTAGGCTGCGTAACTTATAAGGACATATAAGGATAAGAAGCACATACATATAGAGAGGTGAAAATGACGGAACTGGCCAACTATGATATAGATATTTGACTTAGCTCCAACTATGATATAGATATTTGACTTAGCTCCAACTATGATATAGATATTTGACTTAGCTCCATTTTCTCGGTTCAGGTATGGGCAAACCTAATTAGATGCCTACCAATTATAAAACATAGAATAGAGGCAATATGGATCCACCTATATCAAGGGAAGGGTTCAAACTACGGTGGCTATCTACTTTGGGTTTAAATTCCTATGAGTTCTTCTGTATAATTAGACAAAATGTGCGCAAGTTGACTTGTTTACTCGCTGGTTAAAATTAAGAGGACCAACTGATATCAACCCATATGAAGTTCAACGTGAATTCTGTTACTGTTTTATATTCTTCTCTGGCTACAGTACATCGATGACTCGATATTTAGTGATATGTTTTTATCATTCCATTGCAGTGGTATCGGAGCAGGGATGGACATCGTCGTGGCAAGTCTCAAGGCCTACATTGGTGCATTAAACAAGATGCTTGGTTTCTACGGAGTTGATGTTAAGGTGACTGAAAAAATAACACAGTCTGCATAAAAATTGTTTATTTGAGAAGAGGGAGGATGATGGTGCCAATCTTACATTCTCAACATGCTTGAAAGCTTTTAGAATTTTGGGTTAATTTTGCTTTAATTATCAGTATTTAGTTGCTTGTGTTAGACCGCGTTTTAAACTTCTGTGAACTTTGTGTAGTTAAAGTCAAACAAAAAAGCAGGTAGAACATGTTGACTGAGATAAAATTTCGAAGTTAATTAGAAAATAAGAACTCAACACTCATTTAAAGTTTCGTA

mRNA sequence

ATGGCCGTGCCGGTAACTTTGTCGAGTCCGAATCACTTTCTTGTTCGGCATTCGAACTCTCAGTTTCCTTCCTGTCATCGGCGTTACAATCGGAATTTGCAGGCTCTTAAGAATTCTTCACGGTCGTTGTCCTTTAGAACGGTGGCGGACAATGTGTCTCGCATTTACTGCAGTCACACTGAAAGCGTAGTTTCGAATTCCAAATCGAATTCATCCATTAAACGTCCGCCGTATATTCCTAATCGCATCCCTGACTCTTCCTATGTTCGGCTATTTGATACCACTCTCCGCGATGGCGAGCAGTCTCCAGGTGCCTCCCTCACGGCTAAAGAGAAGCTTGATATTGCTCGTCAGCTCGTCAAGCTTGGTGTTGACATAATTGAGGCTGGATTTCCTGCTGCTTCTAAGGAAGACTTTGAGGCCGTCAAGATGATTGCCGCAGACGTTGGCAATGCTGTCAATGAGGACGGATATGTGCCTGTTATTTGTGGTCTTTCGAGGTGTAATGAGAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCACGGATACATACGTTTATAGCAACAAGCGAAATTCATATGGAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGAGATTGCTCGAAATATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTTAGCCCCGAGGATGCTGGGAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGCGAAGTGATAAAGGCTGGTGCAACTACTCTCAACATACCAGATACTGTGGGTTATACAATGCCAGTTGAATTTGGCAACTTGATTGCTGATATAAAAGCTAATACTCCTGGCATTGATAATGTTATCATCTCAACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGGGGCATGTGCAGGCGCAAGGCAACTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCATCGCTAGAGGAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCGTACTGGAATTAACTCGAGACATATCTTTTTGACTAGCAAGATGGTAGAAGAGTACACTGGTTTGAATGTACAACCACACAAGGCAATTGTGGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTCAGTGGACGCCATGCCCTTAAAAGTCGACTTCTAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACGTATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGAGAGTTACAGATATGGATCTTCGAGCATTGGTATCTGATGAGGTTTTACAACCAACTGTTCTATGGAAGCTGCTAGATTTGCAGATCACTTGTGGAACTCTCGGTCTTTCCACTGCTACTGTTAAACTCCTTGATGCCGATGGGAAAGAGCACGTTGCTTGTGCTGTTGGAACGGGCCCTGTAGATTCAGCTTACAAGGCTGTTGATCTAATTATTAAGGAACCAGTAACACTGCTCGATTATTCAATGAATTCTGTCACCGAAGGGATCGATGCAATTGCCACTACTCGTGTTGTAATAAGAGGGGACAACAGTTACTTATCTACTAATGCATCGACTGGAGAAGAAGTTCAACGAACATTTAGTGGTATCGGAGCAGGGATGGACATCGTCGTGGCAAGTCTCAAGGCCTACATTGGTGCATTAAACAAGATGCTTGGTTTCTACGGAGTTGATGTTAAGGTGACTGAAAAAATAACACAGTCTGCATAAAAATTGTTTATTTGAGAAGAGGGAGGATGATGGTGCCAATCTTACATTCTCAACATGCTTGAAAGCTTTTAGAATTTTGGGTTAATTTTGCTTTAATTATCAGTATTTAGTTGCTTGTGTTAGACCGCGTTTTAAACTTCTGTGAACTTTGTGTAGTTAAAGTCAAACAAAAAAGCAGGTAGAACATGTTGACTGAGATAAAATTTCGAAGTTAATTAGAAAATAAGAACTCAACACTCATTTAAAGTTTCGTA

Coding sequence (CDS)

ATGGCCGTGCCGGTAACTTTGTCGAGTCCGAATCACTTTCTTGTTCGGCATTCGAACTCTCAGTTTCCTTCCTGTCATCGGCGTTACAATCGGAATTTGCAGGCTCTTAAGAATTCTTCACGGTCGTTGTCCTTTAGAACGGTGGCGGACAATGTGTCTCGCATTTACTGCAGTCACACTGAAAGCGTAGTTTCGAATTCCAAATCGAATTCATCCATTAAACGTCCGCCGTATATTCCTAATCGCATCCCTGACTCTTCCTATGTTCGGCTATTTGATACCACTCTCCGCGATGGCGAGCAGTCTCCAGGTGCCTCCCTCACGGCTAAAGAGAAGCTTGATATTGCTCGTCAGCTCGTCAAGCTTGGTGTTGACATAATTGAGGCTGGATTTCCTGCTGCTTCTAAGGAAGACTTTGAGGCCGTCAAGATGATTGCCGCAGACGTTGGCAATGCTGTCAATGAGGACGGATATGTGCCTGTTATTTGTGGTCTTTCGAGGTGTAATGAGAATGATATTCGAACTGCATGGGAGGCTGTGAAGTACGCTAAGAGACCACGGATACATACGTTTATAGCAACAAGCGAAATTCATATGGAGCATAAGCTGAGAAAGACGAGGGAGCAAGTGATTGAGATTGCTCGAAATATGGTGCGGTTTGCACGAAGTTTGGGTTGTGATGATGTTGAGTTTAGCCCCGAGGATGCTGGGAGATCTGACAGAGAGTTCCTTTATCAAATTCTGGGCGAAGTGATAAAGGCTGGTGCAACTACTCTCAACATACCAGATACTGTGGGTTATACAATGCCAGTTGAATTTGGCAACTTGATTGCTGATATAAAAGCTAATACTCCTGGCATTGATAATGTTATCATCTCAACACACTGCCAGAATGATCTTGGACTCGCAACTGCCAACACAGTAGCAGGGGCATGTGCAGGCGCAAGGCAACTTGAGGTCACAATTAATGGCATTGGTGAGAGAGCTGGAAATGCATCGCTAGAGGAGGTGGTAATGGCCTTACAATGTCGTGGAGAGCATGTGCTTGGAGGTCTTCGTACTGGAATTAACTCGAGACATATCTTTTTGACTAGCAAGATGGTAGAAGAGTACACTGGTTTGAATGTACAACCACACAAGGCAATTGTGGGAGCTAATGCTTTTGCTCATGAAAGTGGCATCCACCAGGATGGGATGCTTAAGCACAAAGGTACTTATGAAATCATATCCCCTGAAGACATTGGCTACGAACGATCCAATGATGCTGGTATTGTTCTAGGAAAACTCAGTGGACGCCATGCCCTTAAAAGTCGACTTCTAGAGCTTGGTTATGAGCTTGACAATGAGAATCTTGACAACGTATTCTGGCGTTTCAAAGCAGTAGCTGAACAAAAGAAGAGAGTTACAGATATGGATCTTCGAGCATTGGTATCTGATGAGGTTTTACAACCAACTGTTCTATGGAAGCTGCTAGATTTGCAGATCACTTGTGGAACTCTCGGTCTTTCCACTGCTACTGTTAAACTCCTTGATGCCGATGGGAAAGAGCACGTTGCTTGTGCTGTTGGAACGGGCCCTGTAGATTCAGCTTACAAGGCTGTTGATCTAATTATTAAGGAACCAGTAACACTGCTCGATTATTCAATGAATTCTGTCACCGAAGGGATCGATGCAATTGCCACTACTCGTGTTGTAATAAGAGGGGACAACAGTTACTTATCTACTAATGCATCGACTGGAGAAGAAGTTCAACGAACATTTAGTGGTATCGGAGCAGGGATGGACATCGTCGTGGCAAGTCTCAAGGCCTACATTGGTGCATTAAACAAGATGCTTGGTTTCTACGGAGTTGATGTTAAGGTGACTGAAAAAATAACACAGTCTGCATAA

Protein sequence

MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHTESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVASLKAYIGALNKMLGFYGVDVKVTEKITQSA
BLAST of Carg07441 vs. NCBI nr
Match: XP_022948501.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 627/629 (99.68%), Postives = 628/629 (99.84%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60
           MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAA+VGNAVNEDGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420
           IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480
           DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480

Query: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540
           VLQ TVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE
Sbjct: 481 VLQSTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540

Query: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600
           PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS
Sbjct: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600

Query: 601 LKAYIGALNKMLGFYGVDVKVTEKITQSA 630
           LKAYIGALNKMLGFYGVDVKVTEKITQSA
Sbjct: 601 LKAYIGALNKMLGFYGVDVKVTEKITQSA 629

BLAST of Carg07441 vs. NCBI nr
Match: XP_023523405.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 620/629 (98.57%), Postives = 626/629 (99.52%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60
           MAVPVTLSSPNHFLVRHSNSQFPSCHR YNRN QALKNSSRSLSFRTVADNVSRIYCSHT
Sbjct: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRIYNRNFQALKNSSRSLSFRTVADNVSRIYCSHT 60

Query: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ESVVSNSKSNSSI+RPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV
Sbjct: 61  ESVVSNSKSNSSIRRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIAA+VGNAV+ DGYVPVICGLSRCNENDIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAAEVGNAVDSDGYVPVICGLSRCNENDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240
           KYAKRPRIHTFIATSEIHMEHKLRKTREQVI+IARNMVRFARSLGCDDVEFSPEDAGRSD
Sbjct: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIDIARNMVRFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300

Query: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420
           IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480
           DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480

Query: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540
           VLQPTVLWKLLDLQITCGTLGLSTATVKLLDA+GKEHVACAVGTGPVDSAYKAVDLIIKE
Sbjct: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDANGKEHVACAVGTGPVDSAYKAVDLIIKE 540

Query: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600
           PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS
Sbjct: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600

Query: 601 LKAYIGALNKMLGFYGVDVKVTEKITQSA 630
           LKAYIGALNKMLGF+GVDVKVTEKITQSA
Sbjct: 601 LKAYIGALNKMLGFFGVDVKVTEKITQSA 629

BLAST of Carg07441 vs. NCBI nr
Match: XP_023522725.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 590/629 (93.80%), Postives = 607/629 (96.50%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60
           MA+P  LS PN FLVR  NSQF + HR Y+RNLQ LKN SRSLSFRT AD+VSR+YC  T
Sbjct: 1   MAMPAALSRPNQFLVRQPNSQFSARHRIYSRNLQVLKNPSRSLSFRTAADHVSRVYCCQT 60

Query: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           E VV NS+S SSI+RPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEK+DIARQL 
Sbjct: 61  ERVVLNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIARQLA 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIA +VGNAV+EDGYVPVICGLSRCNE DIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240
           KY+KRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMV+FARSLGCDDVEFSPEDAGRSD
Sbjct: 181 KYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGI+NVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQNDL 300

Query: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420
           IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420

Query: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480
           DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE
Sbjct: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480

Query: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540
           VLQPTVLWKLLDLQITCGTLGLSTATVKLLDA+GKEHVACAVGTGPVDSAYKAVDLIIKE
Sbjct: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDANGKEHVACAVGTGPVDSAYKAVDLIIKE 540

Query: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600
           PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS
Sbjct: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600

Query: 601 LKAYIGALNKMLGFYGVDVKVTEKITQSA 630
           LKAYIGALNKMLGF+GVDVKVTEKITQSA
Sbjct: 601 LKAYIGALNKMLGFFGVDVKVTEKITQSA 629

BLAST of Carg07441 vs. NCBI nr
Match: XP_022984140.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 565/629 (89.83%), Postives = 598/629 (95.07%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60
           MA+P   S PN FLVR  NSQF + HR Y+RNLQALKNSSRSLSFRT AD+VSRIYC   
Sbjct: 1   MAMPAAFSRPNQFLVRQPNSQFSARHRIYSRNLQALKNSSRSLSFRTAADHVSRIYCCQK 60

Query: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           ES+VSNS+S SSI+RPPYIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEK+DIARQL 
Sbjct: 61  ESIVSNSESKSSIRRPPYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKVDIARQLT 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIA +VGNAV+EDGYVPVICGLSRCNE DIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240
           KY+KRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMV+FARSLGCDDVEFSPEDAGRSD
Sbjct: 181 KYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGI+NVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQNDL 300

Query: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLATANTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH
Sbjct: 301 GLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420
           IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEII+PEDIGYERSN
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIIAPEDIGYERSN 420

Query: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480
           DAGIVLGKLSGRHALKSRL+ELGYELDNE LDNVFWRFKAVAEQKKRVTD D+RALVSDE
Sbjct: 421 DAGIVLGKLSGRHALKSRLVELGYELDNEKLDNVFWRFKAVAEQKKRVTDADIRALVSDE 480

Query: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540
           V QP VLWKLLDLQ+TCGTLGLSTATVKLLDADG+EH+ACAVGTGPVDSAYKAVDLI+KE
Sbjct: 481 VFQPIVLWKLLDLQVTCGTLGLSTATVKLLDADGEEHIACAVGTGPVDSAYKAVDLIVKE 540

Query: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600
           P TLL+YSM +VTEGIDAIATTRV+IRG+ SY +T+ASTGE VQRTFSGIGAGMDIVV+S
Sbjct: 541 PATLLEYSMTAVTEGIDAIATTRVLIRGEKSYTATHASTGESVQRTFSGIGAGMDIVVSS 600

Query: 601 LKAYIGALNKMLGFYGVDVKVTEKITQSA 630
           +KAYIGALNKMLGF+GVD+KVTEK T SA
Sbjct: 601 VKAYIGALNKMLGFHGVDIKVTEKKTLSA 629

BLAST of Carg07441 vs. NCBI nr
Match: XP_022934784.1 (2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata] >XP_022934785.1 2-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 561/629 (89.19%), Postives = 593/629 (94.28%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60
           MA+P  LS PN FLVR  NSQF + HR Y+RNLQALKN SRSLSFRT  D+VSR+YC  T
Sbjct: 1   MAMPAALSRPNQFLVRQPNSQFSARHRIYSRNLQALKNPSRSLSFRTATDHVSRVYCCQT 60

Query: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
           E VV NS+S SSI+RP YIPNRIPDSSYVR+FDTTLRDGEQSPGASLTAKEKLDIARQL 
Sbjct: 61  ERVVLNSESKSSIRRPSYIPNRIPDSSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLA 120

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAASKEDFEAVKMIA +VGNAV+ DGYVPVICGLSRCNE DIRTAWEAV
Sbjct: 121 KLGVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDADGYVPVICGLSRCNEKDIRTAWEAV 180

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240
           KY+KRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMV+FARSLGCDDVEFSPEDAGRSD
Sbjct: 181 KYSKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVQFARSLGCDDVEFSPEDAGRSD 240

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGI+NVIISTHCQNDL
Sbjct: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISTHCQNDL 300

Query: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GLATANTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGL TGINSRH
Sbjct: 301 GLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRH 360

Query: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420
           IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEII+PEDIGYERSN
Sbjct: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIIAPEDIGYERSN 420

Query: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480
           DAGIVLGKLSGRHALKSRL+ELGYELDNE LDNVFWRFKAVAEQKKRVTD D+RALVSDE
Sbjct: 421 DAGIVLGKLSGRHALKSRLVELGYELDNEKLDNVFWRFKAVAEQKKRVTDADIRALVSDE 480

Query: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540
           V QP VLWKLLDLQ+TCGTLGLSTATVKLLDADG+EH+ACAVGTGPVDSAYKAVDLI+KE
Sbjct: 481 VFQPIVLWKLLDLQVTCGTLGLSTATVKLLDADGEEHIACAVGTGPVDSAYKAVDLIVKE 540

Query: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600
           P TLL+YSM +VTEGIDAIATTRV+IRG+ SY +T+ASTGE VQRTFSGIGAGMDIVV+S
Sbjct: 541 PATLLEYSMTAVTEGIDAIATTRVLIRGEKSYTATHASTGESVQRTFSGIGAGMDIVVSS 600

Query: 601 LKAYIGALNKMLGFYGVDVKVTEKITQSA 630
           +KAYIGALNKMLGF+GVD+KVTEK T SA
Sbjct: 601 VKAYIGALNKMLGFHGVDIKVTEKKTLSA 629

BLAST of Carg07441 vs. TAIR10
Match: AT1G74040.1 (2-isopropylmalate synthase 1)

HSP 1 Score: 914.1 bits (2361), Expect = 4.8e-266
Identity = 458/575 (79.65%), Postives = 519/575 (90.26%), Query Frame = 0

Query: 41  RSLSFRTVA-DNVSRIYCSHTESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDG 100
           RS + RT++     +   S++ S  S    ++  +RP YIPNRI D +YVR+FDTTLRDG
Sbjct: 38  RSQNHRTISLTTAGKFRVSYSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDG 97

Query: 101 EQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYV 160
           EQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPAASK+DFEAVK IA  VGN V+E+GYV
Sbjct: 98  EQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYV 157

Query: 161 PVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVR 220
           PVICGLSRCN+ DI TAWEAVKYAKRPRIHTFIATS+IH+++KL+K++E+VIEIARNMVR
Sbjct: 158 PVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVR 217

Query: 221 FARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD 280
           FARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKAGATTLNIPDTVG T+P EFG LIAD
Sbjct: 218 FARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIAD 277

Query: 281 IKANTPGIDNVIISTHCQNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVM 340
           IKANTPGI NVIISTHCQNDLGL+TANT++GA +GARQ+EVTINGIGERAGNASLEEVVM
Sbjct: 278 IKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVM 337

Query: 341 ALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGM 400
           A++CRG+HVLGGL TGI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQDGM
Sbjct: 338 AIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGM 397

Query: 401 LKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFK 460
           LKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+FWRFK
Sbjct: 398 LKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRFK 457

Query: 461 AVAEQKKRVTDMDLRALVSDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVA 520
           AVAEQKKRVTD DL ALVSDEV QP  +WKLLD+QITCGTLGLST+TVKL D+DGKEHVA
Sbjct: 458 AVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKEHVA 517

Query: 521 CAVGTGPVDSAYKAVDLIIKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAST 580
           C+VGTGPVD+AYKAVDLI+KEP TLL+YSMN+VTEGIDAIATTRV+IRGDN+Y STNA T
Sbjct: 518 CSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAVT 577

Query: 581 GEEVQRTFSGIGAGMDIVVASLKAYIGALNKMLGF 615
           GE V+RTFSG GAGMDIVV+S+KAY+GALNKMLGF
Sbjct: 578 GESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGF 612

BLAST of Carg07441 vs. TAIR10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4)

HSP 1 Score: 907.9 bits (2345), Expect = 3.5e-264
Identity = 446/540 (82.59%), Postives = 498/540 (92.22%), Query Frame = 0

Query: 75  RPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAA 134
           RP YIPNRI D +YVR+FDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 135 SKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 194
           SK+DFEAVK IA  VGN V+E+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 195 SEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKA 254
           S+IH+E+KL+KT+ +VIEIAR+MVRFARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 255 GATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLGLATANTVAGACAG 314
           GATTLNIPDTVG T+P EFG LI D+KANTPGI+NV+ISTHCQNDLGL+TANT++GA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 315 ARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGL 374
           ARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 375 NVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHA 434
             QPHKAIVGANAFAHESGIHQDGMLKHKGTYEII PE+IG ERSNDAGIVLGKLSGRHA
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHA 434

Query: 435 LKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDEVLQPTVLWKLLDLQ 494
           LK RL ELGY+LD+E L  +FWRFK VAEQKKRVTD D+ ALVSDEV QP  +WKLLD+Q
Sbjct: 435 LKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQ 494

Query: 495 ITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKEPVTLLDYSMNSVTE 554
           ITCGTLGLSTATVKL DADGKEHVAC++GTGPVDSAYKAVDLI+KEP TLL+YSMN+VTE
Sbjct: 495 ITCGTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE 554

Query: 555 GIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVASLKAYIGALNKMLGF 614
           GIDAIATTRV+IRG N Y STNA TGEEVQRTFSG GAGMDIVV+S+KAY+GALNKM+ F
Sbjct: 555 GIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDF 614

BLAST of Carg07441 vs. TAIR10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1)

HSP 1 Score: 540.4 bits (1391), Expect = 1.4e-153
Identity = 270/436 (61.93%), Postives = 347/436 (79.59%), Query Frame = 0

Query: 55  IYCSHTESVVSNSKSNSS-----IKR-PPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLT 114
           ++ S   SV  N+ ++S+     ++R P YIPN++PD +YVR+FDTTLRDGEQSPG SLT
Sbjct: 45  LFISCCSSVSKNAATSSTDLKPVVERWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLT 104

Query: 115 AKEKLDIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNED-GYVPVICGLSR 174
             +KL+IARQL KL VDI+E GFP +S+E+ E +K IA  VGN V+E+ GYVPVIC ++R
Sbjct: 105 PPQKLEIARQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVPVICAIAR 164

Query: 175 CNENDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCD 234
           C   DI   WEA+KYAKRPRI  F +TS+IHM++KL+KT+E+VIE+A + +RFA+SLG +
Sbjct: 165 CKHRDIEATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFN 224

Query: 235 DVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGI 294
           D++F  ED GRSD++FL +ILGE IKAG T + I DTVG  MP E+G L+  +KANTPGI
Sbjct: 225 DIQFGCEDGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGI 284

Query: 295 DNVIISTHCQNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEH 354
           D+V+++ HC NDLGLATAN++AG  AGARQ+EVTINGIGER+GNASLEEVVMAL+CRG +
Sbjct: 285 DDVVVAVHCHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAY 344

Query: 355 VLGGLRTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYE 414
           V+ G+ T I++R I  TSKMV+EYTGL VQ HK IVGAN F HESGIHQDG+LK++ TYE
Sbjct: 345 VINGVYTKIDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYE 404

Query: 415 IISPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKR 474
           I+SPEDIG  +S ++G+VLGKLSGRHA+K RL ELGYELD+E L+ VF  F+ + + KKR
Sbjct: 405 ILSPEDIGIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKR 464

Query: 475 VTDMDLRALV--SDEV 482
           +TD DL+ALV  SDE+
Sbjct: 465 ITDADLKALVTSSDEI 480

BLAST of Carg07441 vs. TAIR10
Match: AT5G23020.1 (2-isopropylmalate synthase 2)

HSP 1 Score: 535.0 bits (1377), Expect = 6.1e-152
Identity = 253/405 (62.47%), Postives = 331/405 (81.73%), Query Frame = 0

Query: 74  KRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPA 133
           +RP YIPN++P  +YVR+ DTTLRDGEQSPGA+LT  +KL+IARQL KL VDI+E GFP 
Sbjct: 70  RRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPV 129

Query: 134 ASKEDFEAVKMIAADVGNAVNED-GYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFI 193
           +S+E+FEA+K IA  VGN V+E+ GYVPVICG++RC + DI   WEA+KYAKRPR+  F 
Sbjct: 130 SSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFT 189

Query: 194 ATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVI 253
           +TSEIHM++KL+KT+E+VIE+A N V++A+SLG  D++F  ED GR++++F+ +ILGE I
Sbjct: 190 STSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDIQFGCEDGGRTEKDFICKILGESI 249

Query: 254 KAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLGLATANTVAGAC 313
           KAGATT+   DTVG  MP EFG L+A +  NTPG D+++ + HC NDLG+ATANT++G C
Sbjct: 250 KAGATTVGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHCHNDLGVATANTISGIC 309

Query: 314 AGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYT 373
           AGARQ+EVTINGIGER+GNA LEEVVMAL+CRGE ++ G+ T I+SR I  TSKMV+E+T
Sbjct: 310 AGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKIDSRQIMATSKMVQEHT 369

Query: 374 GLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGR 433
           G+ VQPHK IVG N F HESGIHQDG+LK++ TYEI+SPED+G  +S ++GIVLGKLSGR
Sbjct: 370 GMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEILSPEDVGIVKSENSGIVLGKLSGR 429

Query: 434 HALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALV 478
           HA+K RL ELGYE+ +E  +++F R++ + + KKR+TD DL+ALV
Sbjct: 430 HAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRITDADLKALV 474

BLAST of Carg07441 vs. Swiss-Prot
Match: sp|Q9C550|LEU12_ARATH (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 8.7e-265
Identity = 458/575 (79.65%), Postives = 519/575 (90.26%), Query Frame = 0

Query: 41  RSLSFRTVA-DNVSRIYCSHTESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDG 100
           RS + RT++     +   S++ S  S    ++  +RP YIPNRI D +YVR+FDTTLRDG
Sbjct: 38  RSQNHRTISLTTAGKFRVSYSLSASSPLPPHAPRRRPNYIPNRISDPNYVRIFDTTLRDG 97

Query: 101 EQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYV 160
           EQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPAASK+DFEAVK IA  VGN V+E+GYV
Sbjct: 98  EQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAETVGNTVDENGYV 157

Query: 161 PVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVR 220
           PVICGLSRCN+ DI TAWEAVKYAKRPRIHTFIATS+IH+++KL+K++E+VIEIARNMVR
Sbjct: 158 PVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKKSKEEVIEIARNMVR 217

Query: 221 FARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIAD 280
           FARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKAGATTLNIPDTVG T+P EFG LIAD
Sbjct: 218 FARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIAD 277

Query: 281 IKANTPGIDNVIISTHCQNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVM 340
           IKANTPGI NVIISTHCQNDLGL+TANT++GA +GARQ+EVTINGIGERAGNASLEEVVM
Sbjct: 278 IKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGERAGNASLEEVVM 337

Query: 341 ALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGM 400
           A++CRG+HVLGGL TGI++RHI +TSKMVEEYTG+  QPHKAIVGANAFAHESGIHQDGM
Sbjct: 338 AIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANAFAHESGIHQDGM 397

Query: 401 LKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFK 460
           LKHKGTYEI+SPE+IG ERSNDAGIVLGKLSGRHALK RL ELGY LD+  L N+FWRFK
Sbjct: 398 LKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGRHALKDRLNELGYVLDDGQLSNLFWRFK 457

Query: 461 AVAEQKKRVTDMDLRALVSDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVA 520
           AVAEQKKRVTD DL ALVSDEV QP  +WKLLD+QITCGTLGLST+TVKL D+DGKEHVA
Sbjct: 458 AVAEQKKRVTDADLIALVSDEVFQPEAVWKLLDMQITCGTLGLSTSTVKLADSDGKEHVA 517

Query: 521 CAVGTGPVDSAYKAVDLIIKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNAST 580
           C+VGTGPVD+AYKAVDLI+KEP TLL+YSMN+VTEGIDAIATTRV+IRGDN+Y STNA T
Sbjct: 518 CSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDNNYSSTNAVT 577

Query: 581 GEEVQRTFSGIGAGMDIVVASLKAYIGALNKMLGF 615
           GE V+RTFSG GAGMDIVV+S+KAY+GALNKMLGF
Sbjct: 578 GESVERTFSGTGAGMDIVVSSVKAYVGALNKMLGF 612

BLAST of Carg07441 vs. Swiss-Prot
Match: sp|Q9LPR4|LEU11_ARATH (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 907.9 bits (2345), Expect = 6.2e-263
Identity = 446/540 (82.59%), Postives = 498/540 (92.22%), Query Frame = 0

Query: 75  RPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGFPAA 134
           RP YIPNRI D +YVR+FDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAGFPAA
Sbjct: 75  RPEYIPNRISDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAA 134

Query: 135 SKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTFIAT 194
           SK+DFEAVK IA  VGN V+E+GYVPVICGLSRCN+ DI  AW+AVKYAKRPRIHTFIAT
Sbjct: 135 SKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIAT 194

Query: 195 SEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEVIKA 254
           S+IH+E+KL+KT+ +VIEIAR+MVRFARSLGC+DVEFSPEDAGRS+RE+LY+ILGEVIKA
Sbjct: 195 SDIHLEYKLKKTKAEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKA 254

Query: 255 GATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLGLATANTVAGACAG 314
           GATTLNIPDTVG T+P EFG LI D+KANTPGI+NV+ISTHCQNDLGL+TANT++GA AG
Sbjct: 255 GATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAG 314

Query: 315 ARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEYTGL 374
           ARQ+EVTINGIGERAGNASLEEVVMA++CRG+HVLGGL TGI++RHI +TSKMVEEYTG+
Sbjct: 315 ARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGM 374

Query: 375 NVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSGRHA 434
             QPHKAIVGANAFAHESGIHQDGMLKHKGTYEII PE+IG ERSNDAGIVLGKLSGRHA
Sbjct: 375 QTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHA 434

Query: 435 LKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDEVLQPTVLWKLLDLQ 494
           LK RL ELGY+LD+E L  +FWRFK VAEQKKRVTD D+ ALVSDEV QP  +WKLLD+Q
Sbjct: 435 LKDRLTELGYQLDDEQLSTIFWRFKTVAEQKKRVTDADIIALVSDEVFQPEAVWKLLDIQ 494

Query: 495 ITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKEPVTLLDYSMNSVTE 554
           ITCGTLGLSTATVKL DADGKEHVAC++GTGPVDSAYKAVDLI+KEP TLL+YSMN+VTE
Sbjct: 495 ITCGTLGLSTATVKLADADGKEHVACSIGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTE 554

Query: 555 GIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVASLKAYIGALNKMLGF 614
           GIDAIATTRV+IRG N Y STNA TGEEVQRTFSG GAGMDIVV+S+KAY+GALNKM+ F
Sbjct: 555 GIDAIATTRVLIRGSNKYSSTNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMMDF 614

BLAST of Carg07441 vs. Swiss-Prot
Match: sp|O04973|LEU1A_SOLPN (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 1.4e-246
Identity = 414/543 (76.24%), Postives = 491/543 (90.42%), Query Frame = 0

Query: 72  SIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAGF 131
           S +RP Y+P++I D  YVR+FDTTLRDGEQSPGA++T KEKLD+ARQL KLGVDIIEAGF
Sbjct: 31  SNRRPEYVPSKISDPKYVRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIEAGF 90

Query: 132 PAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHTF 191
           PA+S+ DFE+VK+IA ++GN  +E+G+VPVICGLSRCN++DI  AWEAVKYAK+PR+HTF
Sbjct: 91  PASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPRVHTF 150

Query: 192 IATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGEV 251
           IATSEIHM++KL+ +REQV+E AR+MV +ARSLGC+DVEFSPEDAGRSDREFLY ILGEV
Sbjct: 151 IATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDILGEV 210

Query: 252 IKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLGLATANTVAGA 311
           IKAGATTLNIPDTVGYT+P EFG LI DIKANTPGI+NVIISTHCQNDLGL+TANT+AGA
Sbjct: 211 IKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANTLAGA 270

Query: 312 CAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEEY 371
           CAGARQLEVTINGIGERAGNASLEEVVMAL+CRGE VLGGL TGIN++HI  +SKMVEEY
Sbjct: 271 CAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSKMVEEY 330

Query: 372 TGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLSG 431
           +GL VQPHKAIVGANAFAHESGIHQDGMLKHK TYEIISP+D+G  RSN+AGIVLGKLSG
Sbjct: 331 SGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVLGKLSG 390

Query: 432 RHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDEVLQPTVLWKLL 491
           RHALKS++LELGY++D + L+++FWRFK+VAE+KK++TD DL AL+SDEVLQP V WKL 
Sbjct: 391 RHALKSKMLELGYDIDGKELEDLFWRFKSVAEKKKKITDDDLIALMSDEVLQPNVYWKLG 450

Query: 492 DLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKEPVTLLDYSMNS 551
           D+QI CG+LGLSTATVKL++ DG+EH+AC+VGTGPVD+AYKAVDLI+K P+TLL+YSMN+
Sbjct: 451 DVQIMCGSLGLSTATVKLINTDGQEHIACSVGTGPVDAAYKAVDLIVKVPITLLEYSMNA 510

Query: 552 VTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVASLKAYIGALNKM 611
           VTEGIDAIA+TRV I   + +   N STG+ + RTFSG GA MD+V++S++AYIGALNKM
Sbjct: 511 VTEGIDAIASTRVSICSIDRHTIMNGSTGQTIHRTFSGTGADMDVVISSVRAYIGALNKM 570

Query: 612 LGF 615
           L +
Sbjct: 571 LSY 573

BLAST of Carg07441 vs. Swiss-Prot
Match: sp|O04974|LEU1B_SOLPN (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 8.8e-241
Identity = 409/557 (73.43%), Postives = 490/557 (87.97%), Query Frame = 0

Query: 59  HTESVVSNSKSNSSIKR-PPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIAR 118
           H+ S  S      SI+R P Y P+ IPD +YVR+FDTTLRDGEQSPGA++T KEKLD+AR
Sbjct: 40  HSNSSFSIPVVRCSIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTTKEKLDVAR 99

Query: 119 QLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAW 178
           Q  KLGVDIIEAGFPA+S+ D EAVK+IA +VGN V E+ YVPVICGL+RCN+ DI  AW
Sbjct: 100 QSAKLGVDIIEAGFPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCNKKDIDKAW 159

Query: 179 EAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAG 238
           EAVKYAK+PRIHTFIATSE+HM +KL+ +R+QV+E AR+MV +ARS+GC+DVEFSPEDAG
Sbjct: 160 EAVKYAKKPRIHTFIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDVEFSPEDAG 219

Query: 239 RSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQ 298
           RSD EFLY ILGEVIKAGATTLNIPDTVGYT+P EFG LIA IKANTPG+++VIISTHCQ
Sbjct: 220 RSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQ 279

Query: 299 NDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGIN 358
           NDLGL+TANT+AGACAGARQLEVTINGIGERAGNASLEEVVMAL+CRGE VLGGL TGIN
Sbjct: 280 NDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGIN 339

Query: 359 SRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYE 418
           ++HI ++SKMVE  +GL+VQPHKAIVGANAF HESGIHQDGMLKHK TYEIISPEDIG  
Sbjct: 340 TQHILMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDTYEIISPEDIGLN 399

Query: 419 RSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALV 478
           R+N++GIV GKLSG    K ++LELGYE++ + LD++FWRFK+VAE+KK++TD DL AL+
Sbjct: 400 RANESGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKKKKITDDDLVALM 459

Query: 479 SDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLI 538
           SDEV QP  +W+L ++Q+TCG+LGLSTATVKL+DADG+EH++C+VGTGPVD+AYKAVDLI
Sbjct: 460 SDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGREHISCSVGTGPVDAAYKAVDLI 519

Query: 539 IKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIV 598
           +K PVTLL+YSMN+VT+GIDAIA+TRV+IRG+N + ST+A TGE V RTFSG GA MDIV
Sbjct: 520 VKVPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHALTGETVHRTFSGTGADMDIV 579

Query: 599 VASLKAYIGALNKMLGF 615
           ++S++AY+GALNKM+ F
Sbjct: 580 ISSVRAYVGALNKMMSF 596

BLAST of Carg07441 vs. Swiss-Prot
Match: sp|Q39891|LEU1_SOYBN (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 8.0e-218
Identity = 384/551 (69.69%), Postives = 452/551 (82.03%), Query Frame = 0

Query: 62  SVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVK 121
           S  S+     S  RP YIPN IPDSSYVR+ DTTLRDGEQSPGA++TAKEKLDIARQLVK
Sbjct: 6   STPSSQSPKLSHLRPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVK 65

Query: 122 LGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAVK 181
           LGVDII+ GFP+AS  DF AVKMIA +VGNAV++DGYVPVI G  RC E DI TAWEAVK
Sbjct: 66  LGVDIIQPGFPSASNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVK 125

Query: 182 YAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDR 241
           YAKRPR+ T IATS IHMEHKLRK+++QVI+IAR+MV+FARSLGC+D++F  EDA RSDR
Sbjct: 126 YAKRPRLCTSIATSPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDR 185

Query: 242 EFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLG 301
           EFLY+ILG VI+AGATT+NI DTVG  MP+E G LI DIK NTPGI NVIISTHC NDLG
Sbjct: 186 EFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLG 245

Query: 302 LATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHI 361
           LATANT+ GA  GARQLEVTINGIGERAGNASLEEVVMAL  +G+H L GL T IN+RHI
Sbjct: 246 LATANTIEGARTGARQLEVTINGIGERAGNASLEEVVMALASKGDHALNGLYTRINTRHI 305

Query: 362 FLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSND 421
             TSKMVEEY+G+++QPHK +VGANAF H SGIHQDGMLKHKGTYE ISPE+IG++R+  
Sbjct: 306 LETSKMVEEYSGMHLQPHKPLVGANAFVHASGIHQDGMLKHKGTYETISPEEIGHKRTTR 365

Query: 422 AGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDEV 481
            GIVLGKLSG  AL+ RL ELGY+L  + +D+VFW+FKA+AE+KK VTD+DL+ALVS + 
Sbjct: 366 IGIVLGKLSGSQALRKRLEELGYDLKEDEVDSVFWQFKAMAEKKKVVTDVDLKALVSYKA 425

Query: 482 LQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKEP 541
                +WKL DLQ+TCGT+GLSTATVKL++ DG  HVAC++G G VDS YKA++LI+KEP
Sbjct: 426 FHAESIWKLGDLQVTCGTIGLSTATVKLVNIDGSTHVACSIGIGAVDSTYKAINLIVKEP 485

Query: 542 VTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVASL 601
             LLDYS+NSVTEGI    T RVVI  +N++ ST A T +    TFSGI A MD+VV+++
Sbjct: 486 TKLLDYSLNSVTEGIGVNVTARVVICRENNHTSTYAFTEDANYPTFSGIAAEMDVVVSTV 545

Query: 602 KAYIGALNKML 613
           KAY+ ALNK+L
Sbjct: 546 KAYLVALNKLL 556

BLAST of Carg07441 vs. TrEMBL
Match: tr|A0A1S3C4S6|A0A1S3C4S6_CUCME (2-isopropylmalate synthase 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496846 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 554/630 (87.94%), Postives = 584/630 (92.70%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVA------DNVSR 60
           MA+P  LS PNHFL R S+S FP+ H  Y RN Q LKN SRSLSF+T A      DNVSR
Sbjct: 1   MALPAALSRPNHFLARQSDSLFPARHPIYTRNFQVLKNPSRSLSFKTAAAAAAAPDNVSR 60

Query: 61  IYCSHTESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLD 120
           IYC  TESVVSNS   SS +RPPYIPNRIPD SYVR+FDTTLRDGEQSPGASLT KEKLD
Sbjct: 61  IYCCLTESVVSNSGPKSSTRRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTTKEKLD 120

Query: 121 IARQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIR 180
           IARQL KLGVDIIEAGFPAASKEDFEAVKMIA ++G+AV+EDGYVPVICGLSRCNE DIR
Sbjct: 121 IARQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGSAVDEDGYVPVICGLSRCNEKDIR 180

Query: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPE 240
           TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPE
Sbjct: 181 TAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPE 240

Query: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIIST 300
           DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGI+NVIIST
Sbjct: 241 DAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIIST 300

Query: 301 HCQNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRT 360
           HCQNDLGLATANTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGL T
Sbjct: 301 HCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHT 360

Query: 361 GINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDI 420
           GIN RHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQDGMLKHKGTYEI++PEDI
Sbjct: 361 GINPRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDI 420

Query: 421 GYERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLR 480
           GYERSNDAGIVLGKLSGRHALKSRLLELGYELD+ENL NVFWRFKAVAEQKKRVTD DLR
Sbjct: 421 GYERSNDAGIVLGKLSGRHALKSRLLELGYELDSENLHNVFWRFKAVAEQKKRVTDADLR 480

Query: 481 ALVSDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAV 540
           ALVSDEV QPTVLWKLLDLQ+TCGTLGLSTATVKLLDADGKEH+AC+VGTGPVDSAYKAV
Sbjct: 481 ALVSDEVFQPTVLWKLLDLQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAV 540

Query: 541 DLIIKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGM 600
           DLI+KEP TLL+YSMN+VTEGIDAIATTRV+IRGD SY STNA TGE VQRTFSGIGAGM
Sbjct: 541 DLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGM 600

Query: 601 DIVVASLKAYIGALNKMLGFYGVDVKVTEK 625
           DIVV+S+KAYIGALNKMLGF G+D+K T++
Sbjct: 601 DIVVSSVKAYIGALNKMLGFQGIDIKETKE 630

BLAST of Carg07441 vs. TrEMBL
Match: tr|A0A0A0L7C8|A0A0A0L7C8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 553/633 (87.36%), Postives = 588/633 (92.89%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVA----DNVSRIY 60
           MA+P  LS PNH L R S+S FP+ HR Y+RNLQ LKN SRSLSF+T A     NVS +Y
Sbjct: 1   MALPAALSRPNHSLARQSDSLFPAPHRIYSRNLQVLKNPSRSLSFKTAAASAPHNVSPVY 60

Query: 61  CSHTESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIA 120
              TES+VSNS   +SI+RPPYIPNRIPD SYVR+FDTTLRDGEQSPGASLT KEKLDIA
Sbjct: 61  SCLTESIVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIA 120

Query: 121 RQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTA 180
           RQL KLGVDIIEAGFPAASKEDFEAVKMIA ++GNAV+EDGYVPVICGLSRCNE DIRTA
Sbjct: 121 RQLAKLGVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTA 180

Query: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDA 240
           WEAVKYAKRPRIHTFIATSEIHMEHKLRKT+E+VIEIARNMVRFARSLGCDDVEFSPEDA
Sbjct: 181 WEAVKYAKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDA 240

Query: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHC 300
           GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMP EFG LIADIK+NTPGI+NVIIS+HC
Sbjct: 241 GRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHC 300

Query: 301 QNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGI 360
           QNDLGLATANTVAGACAGARQ+EVTINGIGERAGNASLEEVVMALQCRGEHVLGGL TGI
Sbjct: 301 QNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGI 360

Query: 361 NSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGY 420
           NSRHIFLTSKMVEE+TGLNVQPHKAIVGANAFAH SGIHQDGMLKHKGTYEI++PEDIGY
Sbjct: 361 NSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGY 420

Query: 421 ERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRAL 480
           ERSNDAGIVLGKLSGRHALKS LLELGYELD ENLDNVFWRFKAVAEQKKRVTD DLRAL
Sbjct: 421 ERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRAL 480

Query: 481 VSDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDL 540
           VSDEV QPTVLWKLLD+Q+TCGTLGLSTATVKLLDADGKEH+AC+VGTGPVDSAYKAVDL
Sbjct: 481 VSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSVGTGPVDSAYKAVDL 540

Query: 541 IIKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDI 600
           I+KEP TLL+YSMN+VTEGIDAIATTRV+IRGD SY STNA TGE VQRTFSGIGAGMDI
Sbjct: 541 IVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEAVQRTFSGIGAGMDI 600

Query: 601 VVASLKAYIGALNKMLGFYGVDVKVTEKITQSA 630
           VV+S+KAYIGALNKMLGF G+D+KVTE+ T SA
Sbjct: 601 VVSSVKAYIGALNKMLGFQGIDIKVTEEKTLSA 633

BLAST of Carg07441 vs. TrEMBL
Match: tr|A0A2P4HNS4|A0A2P4HNS4_QUESU (2-isopropylmalate synthase 1, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_52945 PE=3 SV=1)

HSP 1 Score: 939.1 bits (2426), Expect = 5.1e-270
Identity = 473/561 (84.31%), Postives = 516/561 (91.98%), Query Frame = 0

Query: 55  IYCSHTESVVSNS-KSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKL 114
           I CSH +   SNS  + S ++RP YIPNRI D +YVR+FDTTLRDGEQSPGASLT+KEKL
Sbjct: 53  ISCSHPQQTPSNSAPTPSPLRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGASLTSKEKL 112

Query: 115 DIARQLVKLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDI 174
           DIARQL KLGVDIIEAGFPAAS +DFEAVK IA +VGNAV+ DGYVPVICGLSRCNE DI
Sbjct: 113 DIARQLAKLGVDIIEAGFPAASNDDFEAVKAIAKEVGNAVDADGYVPVICGLSRCNEKDI 172

Query: 175 RTAWEAVKYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSP 234
           R AWEAVKYAKRPRIHTFIATS IHME KLRKT+EQVIEIAR+MV FARSLGCDDVEFSP
Sbjct: 173 RRAWEAVKYAKRPRIHTFIATSAIHMEFKLRKTKEQVIEIARSMVSFARSLGCDDVEFSP 232

Query: 235 EDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIIS 294
           EDAGRSD+EFLY+ILGEVIKAGATTLNIPDTVG T+P EFG LI+DIKANTPGI+NVIIS
Sbjct: 233 EDAGRSDKEFLYEILGEVIKAGATTLNIPDTVGITVPTEFGKLISDIKANTPGIENVIIS 292

Query: 295 THCQNDLGLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLR 354
           THCQNDLGL+TANTV+GACAGARQLEVTINGIGERAGNASLEEVVM L+C    VLGGL 
Sbjct: 293 THCQNDLGLSTANTVSGACAGARQLEVTINGIGERAGNASLEEVVMILKCHEGDVLGGLY 352

Query: 355 TGINSRHIFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPED 414
           TGIN+RHI++TSKMVEEYTGL+VQPHKAIVG NAFAHESGIHQDGMLKHKGTYEII+PED
Sbjct: 353 TGINTRHIYITSKMVEEYTGLHVQPHKAIVGPNAFAHESGIHQDGMLKHKGTYEIIAPED 412

Query: 415 IGYERSNDAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDL 474
           IGYERSN+AGIVLGKLSGRHAL+ RL ELGYELDNE L+N+FWRFKAVA+QKKRVTD DL
Sbjct: 413 IGYERSNEAGIVLGKLSGRHALRKRLEELGYELDNEQLENIFWRFKAVADQKKRVTDADL 472

Query: 475 RALVSDEVLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKA 534
           RALVSDEV QP V+WKL DLQ+TCGTLGLSTATVKL+DADG EHVAC+VGTGPVDSAYKA
Sbjct: 473 RALVSDEVFQPEVVWKLHDLQVTCGTLGLSTATVKLIDADGTEHVACSVGTGPVDSAYKA 532

Query: 535 VDLIIKEPVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAG 594
           VDLI+KEPVTLL+YSM +VTEGIDAIATTRV+IRG+NS+ ST+A TGE V RTFSGIGAG
Sbjct: 533 VDLIVKEPVTLLEYSMIAVTEGIDAIATTRVLIRGENSHTSTHAYTGETVHRTFSGIGAG 592

Query: 595 MDIVVASLKAYIGALNKMLGF 615
           MDIVV+S+KAYIGALNKMLGF
Sbjct: 593 MDIVVSSVKAYIGALNKMLGF 613

BLAST of Carg07441 vs. TrEMBL
Match: tr|A0A251NRS1|A0A251NRS1_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G172500 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 1.8e-267
Identity = 473/614 (77.04%), Postives = 535/614 (87.13%), Query Frame = 0

Query: 1   MAVPVTLSSPNHFLVRHSNSQFPSCHRRYNRNLQALKNSSRSLSFRTVADNVSRIYCSHT 60
           +  P ++SS N  + + S +Q        N+N      S+  L   +   +   I C   
Sbjct: 11  LPTPASMSSIN--ISKSSQAQLLFRSHLLNQNKPTFLTSTPHLHTTSKPQSKPHILC--- 70

Query: 61  ESVVSNSKSNSSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLV 120
               S S +  + +RP YIPNRI D +YVR+FDTTLRDGEQSPGASLT+KEKLDIARQL 
Sbjct: 71  ----SQSTNPKAARRPDYIPNRISDPNYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLA 130

Query: 121 KLGVDIIEAGFPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAV 180
           KLGVDIIEAGFPAAS +DFEAVKMIA +VGN+V++DGYVPVICGLSRCN+ DI+ AW+AV
Sbjct: 131 KLGVDIIEAGFPAASNDDFEAVKMIAKEVGNSVDKDGYVPVICGLSRCNKKDIKRAWDAV 190

Query: 181 KYAKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSD 240
           + AKRPRIHTFIATS IHMEHKLRKT+EQV+EIAR+MV +ARSLGCDDVEFSPEDAGRSD
Sbjct: 191 ECAKRPRIHTFIATSAIHMEHKLRKTKEQVVEIARDMVSYARSLGCDDVEFSPEDAGRSD 250

Query: 241 REFLYQILGEVIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDL 300
           REFLYQILGEVIKAGATTLNIPDTVGY +P E+  LI+DIK+NTPGI+NVIISTHCQNDL
Sbjct: 251 REFLYQILGEVIKAGATTLNIPDTVGYNVPSEYSQLISDIKSNTPGIENVIISTHCQNDL 310

Query: 301 GLATANTVAGACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRH 360
           GL+TANT+AGA AGARQLEVTINGIGERAGNASLEEVVM L CRG+HVLGGL TGIN+RH
Sbjct: 311 GLSTANTIAGASAGARQLEVTINGIGERAGNASLEEVVMTLDCRGDHVLGGLYTGINARH 370

Query: 361 IFLTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 420
           I++TSKMVEEYTGL+VQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN
Sbjct: 371 IYITSKMVEEYTGLHVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSN 430

Query: 421 DAGIVLGKLSGRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDE 480
           +AGIVLGKLSGRHALK RL ELGYEL ++ L+ +FWRFKAVA+QKKRVTD DLRALVSDE
Sbjct: 431 EAGIVLGKLSGRHALKKRLEELGYELADDQLETIFWRFKAVADQKKRVTDADLRALVSDE 490

Query: 481 VLQPTVLWKLLDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKE 540
           V QP V+WKL DLQ+TCGTLGLSTATVKL+DADG+EHVACAVGTGPVDSAYKAVDLI+KE
Sbjct: 491 VFQPEVVWKLHDLQVTCGTLGLSTATVKLIDADGREHVACAVGTGPVDSAYKAVDLIVKE 550

Query: 541 PVTLLDYSMNSVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVAS 600
           PV LL+YSMN+VTEGIDAIATTRV+IRG+NS ++T+A TGE VQRTFSGIGAGMDIVV+S
Sbjct: 551 PVILLEYSMNAVTEGIDAIATTRVLIRGENSNITTHAFTGETVQRTFSGIGAGMDIVVSS 610

Query: 601 LKAYIGALNKMLGF 615
           +KAY+GALNKMLGF
Sbjct: 611 VKAYVGALNKMLGF 615

BLAST of Carg07441 vs. TrEMBL
Match: tr|A0A1U8HLM1|A0A1U8HLM1_GOSHI (2-isopropylmalate synthase 2, chloroplastic-like OS=Gossypium hirsutum OX=3635 GN=LOC107887273 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 1.2e-266
Identity = 462/544 (84.93%), Postives = 509/544 (93.57%), Query Frame = 0

Query: 71  SSIKRPPYIPNRIPDSSYVRLFDTTLRDGEQSPGASLTAKEKLDIARQLVKLGVDIIEAG 130
           SS +RPPYIPN IPD +YVR+FDTTLRDGEQSPGA+LT+KEKLDIARQL KLGVDIIEAG
Sbjct: 62  SSQRRPPYIPNHIPDPNYVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAG 121

Query: 131 FPAASKEDFEAVKMIAADVGNAVNEDGYVPVICGLSRCNENDIRTAWEAVKYAKRPRIHT 190
           FPAASK+DFEAVK IA +VGNAV+ +GYVPVICGLSRCNE DI+TAWEAVKYAKRPRIHT
Sbjct: 122 FPAASKDDFEAVKTIAKEVGNAVDGNGYVPVICGLSRCNEKDIKTAWEAVKYAKRPRIHT 181

Query: 191 FIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDREFLYQILGE 250
           FIATS IH+E+KLRK++E+V++IARNMVRFARSLGC+DVEFSPEDAGRSDREFLY+ILGE
Sbjct: 182 FIATSAIHLEYKLRKSKEEVLDIARNMVRFARSLGCNDVEFSPEDAGRSDREFLYEILGE 241

Query: 251 VIKAGATTLNIPDTVGYTMPVEFGNLIADIKANTPGIDNVIISTHCQNDLGLATANTVAG 310
           VIKAGATTLNIPDTVG TMP EFG LIADIKANTPGI+NVIISTHCQNDLGL+TANT+AG
Sbjct: 242 VIKAGATTLNIPDTVGITMPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANTIAG 301

Query: 311 ACAGARQLEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLRTGINSRHIFLTSKMVEE 370
           ACAGARQ+EVTINGIGERAGNAS+EEVVMAL+CRGEHVLGGL TGIN+RHI + SKMVEE
Sbjct: 302 ACAGARQVEVTINGIGERAGNASMEEVVMALKCRGEHVLGGLHTGINTRHIVMASKMVEE 361

Query: 371 YTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDAGIVLGKLS 430
           YTGL+VQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIG ERSN+AGIVLGKLS
Sbjct: 362 YTGLHVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNEAGIVLGKLS 421

Query: 431 GRHALKSRLLELGYELDNENLDNVFWRFKAVAEQKKRVTDMDLRALVSDEVLQPTVLWKL 490
           GRHAL  RL ELGYELD+  L N+FWRFK VAEQKKRVTD DL ALVSDEV QP V+WKL
Sbjct: 422 GRHALSDRLKELGYELDDVQLGNIFWRFKEVAEQKKRVTDADLVALVSDEVFQPDVVWKL 481

Query: 491 LDLQITCGTLGLSTATVKLLDADGKEHVACAVGTGPVDSAYKAVDLIIKEPVTLLDYSMN 550
           LDLQ+TCGTLGLSTATVKL+DADG++HVAC+VGTGPVDSAYKAVDLIIKE V+LL+YSMN
Sbjct: 482 LDLQVTCGTLGLSTATVKLIDADGRKHVACSVGTGPVDSAYKAVDLIIKESVSLLEYSMN 541

Query: 551 SVTEGIDAIATTRVVIRGDNSYLSTNASTGEEVQRTFSGIGAGMDIVVASLKAYIGALNK 610
           +VTEGIDAIATTRV+IR + S+LST+A TGE V RTFSG GAGMDIVV+S+KAY+ ALNK
Sbjct: 542 AVTEGIDAIATTRVLIRVEKSHLSTHALTGELVHRTFSGTGAGMDIVVSSVKAYVSALNK 601

Query: 611 MLGF 615
           MLGF
Sbjct: 602 MLGF 605

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948501.10.0e+0099.682-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata][more]
XP_023523405.10.0e+0098.572-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023522725.10.0e+0093.802-isopropylmalate synthase 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022984140.10.0e+0089.832-isopropylmalate synthase 2, chloroplastic-like [Cucurbita maxima][more]
XP_022934784.10.0e+0089.192-isopropylmalate synthase 2, chloroplastic-like [Cucurbita moschata] >XP_022934... [more]
Match NameE-valueIdentityDescription
AT1G74040.14.8e-26679.652-isopropylmalate synthase 1[more]
AT1G18500.13.5e-26482.59methylthioalkylmalate synthase-like 4[more]
AT5G23010.11.4e-15361.93methylthioalkylmalate synthase 1[more]
AT5G23020.16.1e-15262.472-isopropylmalate synthase 2[more]
Match NameE-valueIdentityDescription
sp|Q9C550|LEU12_ARATH8.7e-26579.652-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I... [more]
sp|Q9LPR4|LEU11_ARATH6.2e-26382.592-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I... [more]
sp|O04973|LEU1A_SOLPN1.4e-24676.242-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1[more]
sp|O04974|LEU1B_SOLPN8.8e-24173.432-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1[more]
sp|Q39891|LEU1_SOYBN8.0e-21869.69Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3C4S6|A0A1S3C4S6_CUCME0.0e+0087.942-isopropylmalate synthase 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
tr|A0A0A0L7C8|A0A0A0L7C8_CUCSA0.0e+0087.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1[more]
tr|A0A2P4HNS4|A0A2P4HNS4_QUESU5.1e-27084.312-isopropylmalate synthase 1, chloroplastic OS=Quercus suber OX=58331 GN=CFP56_5... [more]
tr|A0A251NRS1|A0A251NRS1_PRUPE1.8e-26777.04Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G172500 PE=3 SV=1[more]
tr|A0A1U8HLM1|A0A1U8HLM1_GOSHI1.2e-26684.932-isopropylmalate synthase 2, chloroplastic-like OS=Gossypium hirsutum OX=3635 G... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0046912transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
GO:0003824catalytic activity
GO:00038522-isopropylmalate synthase activity
Vocabulary: Biological Process
TermDefinition
GO:0019752carboxylic acid metabolic process
GO:0009098leucine biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR036230LeuA_allosteric_dom_sf
IPR002034AIPM/Hcit_synth_CS
IPR000891PYR_CT
IPR013785Aldolase_TIM
IPR005671LeuA_bact_synth
IPR0137092-isopropylmalate_synth_dimer
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0009098 leucine biosynthetic process
biological_process GO:0006090 pyruvate metabolic process
biological_process GO:0009099 valine biosynthetic process
biological_process GO:0019752 carboxylic acid metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003852 2-isopropylmalate synthase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0046912 transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg07441-RACarg07441-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0137092-isopropylmalate synthase LeuA, allosteric (dimerisation) domainSMARTSM00917LeuA_dimer_2coord: 467..612
e-value: 9.6E-30
score: 114.8
IPR0137092-isopropylmalate synthase LeuA, allosteric (dimerisation) domainPFAMPF08502LeuA_dimercoord: 468..612
e-value: 5.5E-38
score: 129.8
NoneNo IPR availableGENE3DG3DSA:1.10.238.260coord: 375..487
e-value: 1.8E-36
score: 126.9
NoneNo IPR availableGENE3DG3DSA:3.30.160.270coord: 498..612
e-value: 5.8E-9
score: 37.7
NoneNo IPR availablePANTHERPTHR10277:SF92-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATEDcoord: 87..611
NoneNo IPR availablePANTHERPTHR10277HOMOCITRATE SYNTHASE-RELATEDcoord: 87..611
NoneNo IPR availableCDDcd07940DRE_TIM_IPMScoord: 92..370
e-value: 4.84615E-174
score: 497.741
NoneNo IPR availableSUPERFAMILYSSF51569Aldolasecoord: 87..386
IPR0056712-isopropylmalate synthase, bacterial-typeTIGRFAMTIGR00973TIGR00973coord: 89..609
e-value: 1.7E-198
score: 658.1
IPR0056712-isopropylmalate synthase, bacterial-typeHAMAPMF_01025LeuA_type1coord: 86..621
score: 39.487
IPR013785Aldolase-type TIM barrelGENE3DG3DSA:3.20.20.70coord: 85..371
e-value: 2.6E-106
score: 356.9
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 89..368
e-value: 3.6E-92
score: 308.6
IPR000891Pyruvate carboxyltransferasePROSITEPS50991PYR_CTcoord: 89..362
score: 40.94
IPR002034Alpha-isopropylmalate/homocitrate synthase, conserved sitePROSITEPS00815AIPM_HOMOCIT_SYNTH_1coord: 96..112
IPR0362302-isopropylmalate synthase LeuA, allosteric (dimerisation) domain superfamilySUPERFAMILYSSF1109212-isopropylmalate synthase LeuA, allosteric (dimerisation) domaincoord: 493..611