Lsi04G022310 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G022310
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Microsomal signal peptidase 12 kDa protein
Location	chr04 : 29354162 .. 29354560 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACGACGCCGCCCTCAACTCCTCTCTGGTTTTCTTGACCGCCGCCGTCGTTCTCGTCGGGATTACCACGTGGTCCTTCAAGAAGATGGCCGTAACTTATTTCGTCGGCGTTTTCGCCATCGCCGGCGTCCTCTTGCCGGATTGGTGCTTCTTTGACCGCGATTTCTCTCGGTGGACTTCTCCGGTCACCGAAGAGGAAAGGGAATTGTATCGGATCTCGACTGCATCTCGGTTTCGAAGGTCCGTTTTAATTTCTTGTGCTCTTTTTTCTTCTTTGATTGATGAAAAATTGTGCGAGAAAATTAGGTTCAGTATTTTTGCAGCCTTACGAAAATTAATTTCGAGTTTCAAGGAGCACTCTCCCGGACAGAATAAATTACCTCTTGTGGAATAA

mRNA sequence

ATGGCCAACGACGCCGCCCTCAACTCCTCTCTGGTTTTCTTGACCGCCGCCGTCGTTCTCGTCGGGATTACCACGTGGTCCTTCAAGAAGATGGCCGTAACTTATTTCGTCGGCGTTTTCGCCATCGCCGGCGTCCTCTTGCCGGATTGGTGCTTCTTTGACCGCGATTTCTCTCGGTGGACTTCTCCGGTCACCGAAGAGGAAAGGGAATTGTATCGGATCTCGACTGCATCTCGGTTTCGAAGGTTCAGTATTTTTGCAGCCTTACGAAAATTAATTTCGAGTTTCAAGGAGCACTCTCCCGGACAGAATAAATTACCTCTTGTGGAATAA

Coding sequence (CDS)

ATGGCCAACGACGCCGCCCTCAACTCCTCTCTGGTTTTCTTGACCGCCGCCGTCGTTCTCGTCGGGATTACCACGTGGTCCTTCAAGAAGATGGCCGTAACTTATTTCGTCGGCGTTTTCGCCATCGCCGGCGTCCTCTTGCCGGATTGGTGCTTCTTTGACCGCGATTTCTCTCGGTGGACTTCTCCGGTCACCGAAGAGGAAAGGGAATTGTATCGGATCTCGACTGCATCTCGGTTTCGAAGGTTCAGTATTTTTGCAGCCTTACGAAAATTAATTTCGAGTTTCAAGGAGCACTCTCCCGGACAGAATAAATTACCTCTTGTGGAATAA

Protein sequence

MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRWTSPVTEEERELYRISTASRFRRFSIFAALRKLISSFKEHSPGQNKLPLVE

Homology

[Close]

BLAST of Lsi04G022310 vs. Swiss-Prot
Match: DTM1_ORYSJ (Signal peptidase complex-like protein DTM1 OS=Oryza sativa subsp. japonica GN=DTM1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 4.1e-13
Identity = 38/69 (55.07%), Postives = 46/69 (66.67%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          M  D  L  SLV L AAVV+ G+ T S +K A TY  G+ AIAGVLLPDW FFDRD+S+W
Sbjct: 1  MGRDEMLRRSLVALAAAVVVTGVVTASVRKAAATYGFGILAIAGVLLPDWEFFDRDYSQW 60

Query: 61 TSPVTEEER 70
           +P+    R
Sbjct: 61 LTPMPASRR 69

[Close]

BLAST of Lsi04G022310 vs. TrEMBL
Match: A0A0A0LGU6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889170 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 3.4e-30
Identity = 70/86 (81.40%), Postives = 76/86 (88.37%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDAALNSSLV L  AV+LVGI+T+SFKKMAVTYFVGVFAIAGVLLPDWCFF RDFSRW
Sbjct: 1  MANDAALNSSLVILALAVLLVGISTYSFKKMAVTYFVGVFAIAGVLLPDWCFFGRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRFSIF 87
          TSPVTEEERE YR +T S  +RF I+
Sbjct: 61 TSPVTEEERESYRNATGSHLQRFRIY 86

[Close]

BLAST of Lsi04G022310 vs. TrEMBL
Match: W9RMI1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_003402 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.0e-18
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDAAL S+LV+L   V++VGI T+SFKKM  TY VGV  I+GV LPDW +FDRDFSRW
Sbjct: 1  MANDAALRSALVWLAGVVLVVGICTFSFKKMLATYVVGVVGISGVFLPDWGYFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRFSIF 87
          TSPVT EER  + I+  S   RF I+
Sbjct: 61 TSPVTAEERASH-IAQRSGPTRFKIY 85

[Close]

BLAST of Lsi04G022310 vs. TrEMBL
Match: M5Y0M4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023975mg PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-17
Identity = 49/83 (59.04%), Postives = 60/83 (72.29%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDA   +SLV+L A +V+VG+ T SFKKM +TY VGV  I+G+LLPDW +FDRDFSRW
Sbjct: 1  MANDAVFRASLVWLAAVIVVVGVFTQSFKKMMLTYVVGVLGISGLLLPDWGYFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRF 84
          TSPV+ EER       +   RRF
Sbjct: 61 TSPVSAEERASEVAQRSGLIRRF 83

[Close]

BLAST of Lsi04G022310 vs. TrEMBL
Match: A0A067LA48_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15787 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-17
Identity = 46/67 (68.66%), Postives = 56/67 (83.58%), Query Frame = 1


Query: 3  NDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRWTS 62
          NDAAL +SLV+L A +V+VGI T S KKMAVTY +G+  IAG+LLPDW +FDRDFSRW S
Sbjct: 4  NDAALRASLVWLAAVMVVVGIWTQSLKKMAVTYIIGIVGIAGLLLPDWDYFDRDFSRWCS 63

Query: 63 PVTEEER 70
          P+TEEE+
Sbjct: 64 PITEEEK 70

[Close]

BLAST of Lsi04G022310 vs. TrEMBL
Match: M5Y1Z1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025664mg PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-17
Identity = 49/83 (59.04%), Postives = 59/83 (71.08%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDA   +SLV+L A +V+VG+ T SFKKM  TY VGV  I+G+LLPDW +FDRDFSRW
Sbjct: 1  MANDAVFRASLVWLAAVIVVVGVCTQSFKKMMYTYVVGVLGISGLLLPDWGYFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRF 84
          TSPV+ EER       +   RRF
Sbjct: 61 TSPVSAEERASEVAQRSGLIRRF 83

[Close]

BLAST of Lsi04G022310 vs. TAIR10
Match: AT1G34640.1 (AT1G34640.1 peptidases)

HSP 1 Score: 84.3 bits (207), Expect = 5.0e-17
Identity = 48/83 (57.83%), Postives = 55/83 (66.27%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          M N+AAL SS+V L   +V+V + T S KK  VTY VG+  I G+LLPDW FFDR FSRW
Sbjct: 1  MGNEAALRSSMVGLAVVMVVVWLWTQSLKKTVVTYAVGISLIGGILLPDWDFFDRSFSRW 60

Query: 61 TSPVTEEERE--LYRISTASRFR 82
            PVT EER   L R S  SRFR
Sbjct: 61 GYPVTAEERSAALARKSHPSRFR 83

[Close]

BLAST of Lsi04G022310 vs. NCBI nr
Match: gi|659132508|ref|XP_008466235.1| (PREDICTED: probable signal peptidase complex subunit 1 [Cucumis melo])

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-31
Identity = 72/86 (83.72%), Postives = 78/86 (90.70%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDAALNSSLV L  A+VLVGITT+SFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW
Sbjct: 1  MANDAALNSSLVILALAIVLVGITTYSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRFSIF 87
          TSPVTEEERE YR +T S+ +RF I+
Sbjct: 61 TSPVTEEERESYRNATGSQLQRFRIY 86

[Close]

BLAST of Lsi04G022310 vs. NCBI nr
Match: gi|449437024|ref|XP_004136292.1| (PREDICTED: uncharacterized protein LOC101214135 [Cucumis sativus])

HSP 1 Score: 139.0 bits (349), Expect = 4.8e-30
Identity = 70/86 (81.40%), Postives = 76/86 (88.37%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDAALNSSLV L  AV+LVGI+T+SFKKMAVTYFVGVFAIAGVLLPDWCFF RDFSRW
Sbjct: 1  MANDAALNSSLVILALAVLLVGISTYSFKKMAVTYFVGVFAIAGVLLPDWCFFGRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRFSIF 87
          TSPVTEEERE YR +T S  +RF I+
Sbjct: 61 TSPVTEEERESYRNATGSHLQRFRIY 86

[Close]

BLAST of Lsi04G022310 vs. NCBI nr
Match: gi|703127816|ref|XP_010103942.1| (hypothetical protein L484_003402 [Morus notabilis])

HSP 1 Score: 100.9 bits (250), Expect = 1.5e-18
Identity = 54/86 (62.79%), Postives = 64/86 (74.42%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDAAL S+LV+L   V++VGI T+SFKKM  TY VGV  I+GV LPDW +FDRDFSRW
Sbjct: 1  MANDAALRSALVWLAGVVLVVGICTFSFKKMLATYVVGVVGISGVFLPDWGYFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRFSIF 87
          TSPVT EER  + I+  S   RF I+
Sbjct: 61 TSPVTAEERASH-IAQRSGPTRFKIY 85

[Close]

BLAST of Lsi04G022310 vs. NCBI nr
Match: gi|645225104|ref|XP_008219427.1| (PREDICTED: probable signal peptidase complex subunit 1 [Prunus mume])

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-17
Identity = 49/83 (59.04%), Postives = 60/83 (72.29%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDA   +SLV+L A +V+VG+ T SFKKM +TY VGV  I+G+LLPDW +FDRDFSRW
Sbjct: 1  MANDAVFRASLVWLAAVIVVVGVCTESFKKMLLTYVVGVLGISGLLLPDWGYFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRF 84
          TSPV+ EER       +   RRF
Sbjct: 61 TSPVSAEERASEVAQRSGLIRRF 83

[Close]

BLAST of Lsi04G022310 vs. NCBI nr
Match: gi|596292904|ref|XP_007226579.1| (hypothetical protein PRUPE_ppa023975mg [Prunus persica])

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-17
Identity = 49/83 (59.04%), Postives = 60/83 (72.29%), Query Frame = 1


Query: 1  MANDAALNSSLVFLTAAVVLVGITTWSFKKMAVTYFVGVFAIAGVLLPDWCFFDRDFSRW 60
          MANDA   +SLV+L A +V+VG+ T SFKKM +TY VGV  I+G+LLPDW +FDRDFSRW
Sbjct: 1  MANDAVFRASLVWLAAVIVVVGVFTQSFKKMMLTYVVGVLGISGLLLPDWGYFDRDFSRW 60

Query: 61 TSPVTEEERELYRISTASRFRRF 84
          TSPV+ EER       +   RRF
Sbjct: 61 TSPVSAEERASEVAQRSGLIRRF 83

The following BLAST results are available for this feature:

BLAST of Lsi04G022310 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DTM1_ORYSJ	4.1e-13	55.07	Signal peptidase complex-like protein DTM1 OS=Oryza sativa subsp. japonica GN=DT...	[more]

BLAST of Lsi04G022310 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LGU6_CUCSA	3.4e-30	81.40	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889170 PE=4 SV=1	[more]
W9RMI1_9ROSA	1.0e-18	62.79	Uncharacterized protein OS=Morus notabilis GN=L484_003402 PE=4 SV=1	[more]
M5Y0M4_PRUPE	1.1e-17	59.04	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023975mg PE=4 SV=1	[more]
A0A067LA48_JATCU	1.1e-17	68.66	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15787 PE=4 SV=1	[more]
M5Y1Z1_PRUPE	1.5e-17	59.04	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa025664mg PE=4 SV=1	[more]

BLAST of Lsi04G022310 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G34640.1	5.0e-17	57.83	peptidases	[more]

BLAST of Lsi04G022310 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659132508\|ref\|XP_008466235.1\|	1.1e-31	83.72	PREDICTED: probable signal peptidase complex subunit 1 [Cucumis melo]	[more]
gi\|449437024\|ref\|XP_004136292.1\|	4.8e-30	81.40	PREDICTED: uncharacterized protein LOC101214135 [Cucumis sativus]	[more]
gi\|703127816\|ref\|XP_010103942.1\|	1.5e-18	62.79	hypothetical protein L484_003402 [Morus notabilis]	[more]
gi\|645225104\|ref\|XP_008219427.1\|	1.6e-17	59.04	PREDICTED: probable signal peptidase complex subunit 1 [Prunus mume]	[more]
gi\|596292904\|ref\|XP_007226579.1\|	1.6e-17	59.04	hypothetical protein PRUPE_ppa023975mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane
GO:0005787	signal peptidase complex

Vocabulary: **Molecular Function**
Term	Definition
GO:0008233	peptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006465	signal peptide processing

Vocabulary: **INTERPRO**
Term	Definition
IPR009542	SPC12

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006465	signal peptide processing
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005787	signal peptidase complex
molecular_function	GO:0008233	peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G022310.1	Lsi04G022310.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009542	Microsomal signal peptidase 12kDa subunit	PFAM	PF06645	SPC12	coord: 8..63 score: 1.
None	No IPR available	PANTHER	PTHR38354	FAMILY NOT NAMED	coord: 2..85 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G022310	Csa3G889170	Cucumber (Chinese Long) v2	culsiB191
Lsi04G022310	CSPI03G45050	Wild cucumber (PI 183967)	cpilsiB189
Lsi04G022310	Cucsa.311450	Cucumber (Gy14) v1	cgylsiB503
Lsi04G022310	CmaCh01G016030	Cucurbita maxima (Rimu)	cmalsiB425
Lsi04G022310	CmoCh01G016500	Cucurbita moschata (Rifu)	cmolsiB413
Lsi04G022310	Cla023009	Watermelon (97103) v1	lsiwmB334
Lsi04G022310	MELO3C003576	Melon (DHL92) v3.5.1	lsimeB310
Lsi04G022310	ClCG11G014080	Watermelon (Charleston Gray)	lsiwcgB260
Lsi04G022310	MELO3C003576.2	Melon (DHL92) v3.6.1	lsimedB324
Lsi04G022310	Cla97C11G220060	Watermelon (97103) v2	lsiwmbB267
Lsi04G022310	Bhi09G002058	Wax gourd	lsiwgoB393
Lsi04G022310	Cp4.1LG02g04960	Cucurbita pepo (Zucchini)	cpelsiB463
Lsi04G022310	CsGy3G042530	Cucumber (Gy14) v2	cgyblsiB175
Lsi04G022310	Carg25627	Silver-seed gourd	carlsiB922
Lsi04G022310	CsaV3_3G047480	Cucumber (Chinese Long) v3	cuclsiB208

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G022310	Bottle gourd (USVL1VR-Ls)	lsilsiB154
Lsi04G022310	Cucurbita maxima (Rimu)	cmalsiB022
Lsi04G022310	Cucurbita moschata (Rifu)	cmolsiB010
Lsi04G022310	Cucurbita pepo (Zucchini)	cpelsiB636
Lsi04G022310	Cucumber (Gy14) v2	cgyblsiB112
Lsi04G022310	Silver-seed gourd	carlsiB575
Lsi04G022310	Watermelon (97103) v2	lsiwmbB309
Lsi04G022310	Wax gourd	lsiwgoB358