Lsi04G020490 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G020490
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Ethylene-responsive transcription factor 6
Location	chr04 : 27600605 .. 27601207 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCGTAGACCGGTCGACATCCACCGCCTCAACCGCCGCGCCGCCCAATTCTAAAGAGATCCGCTACAGGGGCGTTAGAAAGAGGCCGTGGGGGCGCTACGCCGCGGAAATCAGGGACCCACGTAAGAAGACTAGGGTTTGGCTTGGCACATTCAACACCGCTGAAGAGGCGGCGCGTGCTTATGACACGGCCGCGCGTGAATTTAGAGGCGCAAATGCCAAAACTAACTTCCCCATTCAGAACGAATTCCTATTCGTCGACAACAATAACAACTTCCGTAAATCCGCTTGCAGCAGTAGTCCCAGCCAGTGCAGCACCGTCGACTCCTCTTCCCCCTCGCCAGCGCCGTCGGATCCCTCCCTCACTCACTTCCTCGACGCCGGCGTCCACGCGCCGGCCATTTCCCGCTCAATTTTCTTCTTCGACGCGTTCGCTCAGGCGGAGAAGATCAGTGACTTTCGTCGGACCGTTACCGTCGCCGGTACCGGCGGATCTGTGAGCGACTCCGATTCGTCATCCGTAGTTGATTTCGACACTACGCGTAAATCTCGGCCGTTGGATTTTGACCTGAATCTTCCGGCTGTGGAAGTTGTCTGA

mRNA sequence

ATGGCTGCCGTAGACCGGTCGACATCCACCGCCTCAACCGCCGCGCCGCCCAATTCTAAAGAGATCCGCTACAGGGGCGTTAGAAAGAGGCCGTGGGGGCGCTACGCCGCGGAAATCAGGGACCCACGTAAGAAGACTAGGGTTTGGCTTGGCACATTCAACACCGCTGAAGAGGCGGCGCGTGCTTATGACACGGCCGCGCGTGAATTTAGAGGCGCAAATGCCAAAACTAACTTCCCCATTCAGAACGAATTCCTATTCGTCGACAACAATAACAACTTCCGTAAATCCGCTTGCAGCAGTAGTCCCAGCCAGTGCAGCACCGTCGACTCCTCTTCCCCCTCGCCAGCGCCGTCGGATCCCTCCCTCACTCACTTCCTCGACGCCGGCGTCCACGCGCCGGCCATTTCCCGCTCAATTTTCTTCTTCGACGCGTTCGCTCAGGCGGAGAAGATCAGTGACTTTCGTCGGACCGTTACCGTCGCCGGTACCGGCGGATCTGTGAGCGACTCCGATTCGTCATCCGTAGTTGATTTCGACACTACGCGTAAATCTCGGCCGTTGGATTTTGACCTGAATCTTCCGGCTGTGGAAGTTGTCTGA

Coding sequence (CDS)

ATGGCTGCCGTAGACCGGTCGACATCCACCGCCTCAACCGCCGCGCCGCCCAATTCTAAAGAGATCCGCTACAGGGGCGTTAGAAAGAGGCCGTGGGGGCGCTACGCCGCGGAAATCAGGGACCCACGTAAGAAGACTAGGGTTTGGCTTGGCACATTCAACACCGCTGAAGAGGCGGCGCGTGCTTATGACACGGCCGCGCGTGAATTTAGAGGCGCAAATGCCAAAACTAACTTCCCCATTCAGAACGAATTCCTATTCGTCGACAACAATAACAACTTCCGTAAATCCGCTTGCAGCAGTAGTCCCAGCCAGTGCAGCACCGTCGACTCCTCTTCCCCCTCGCCAGCGCCGTCGGATCCCTCCCTCACTCACTTCCTCGACGCCGGCGTCCACGCGCCGGCCATTTCCCGCTCAATTTTCTTCTTCGACGCGTTCGCTCAGGCGGAGAAGATCAGTGACTTTCGTCGGACCGTTACCGTCGCCGGTACCGGCGGATCTGTGAGCGACTCCGATTCGTCATCCGTAGTTGATTTCGACACTACGCGTAAATCTCGGCCGTTGGATTTTGACCTGAATCTTCCGGCTGTGGAAGTTGTCTGA

Protein sequence

MAAVDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSDPSLTHFLDAGVHAPAISRSIFFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNLPAVEVV

Homology

[Close]

BLAST of Lsi04G020490 vs. Swiss-Prot
Match: ERF78_ARATH (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana GN=ERF4 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 103/203 (50.74%), Postives = 123/203 (60.59%), Query Frame = 1


Query: 18  NSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKT 77
           N+KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAAR+FRGA AKT
Sbjct: 19  NAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKAKT 78

Query: 78  NFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSP--APS------DPSLTHFLDA 137
           NFP      F++ ++    +  + SPSQ ST+D +SP     PS       P L   L  
Sbjct: 79  NFPT-----FLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLELSLGG 138

Query: 138 G----VHAPAISRSIFFFDAFAQAEK--------ISDFRRTVTVAGTG---GSVSDSDSS 196
           G     +   +SR ++F D                S FR  V    T    G+ SDSDSS
Sbjct: 139 GGGGSCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMACGAQSDSDSS 198

[Close]

BLAST of Lsi04G020490 vs. Swiss-Prot
Match: ERF79_ARATH (Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana GN=ERF8 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.9e-26
Identity = 88/184 (47.83%), Postives = 107/184 (58.15%), Query Frame = 1


Query: 19  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTN 78
           +KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TA++AARAYD AAR+FRG  AKTN
Sbjct: 26  AKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAAARDFRGVKAKTN 85

Query: 79  FPIQNEFLFVDNNNNFRKSACSSSPSQCST-VDSSSPSPAPSDPSLTHFLDAGVHAPAIS 138
           F +                   SSP+Q ST VDS + +   + P L   L  G    A  
Sbjct: 86  FGV----------------IVGSSPTQSSTVVDSPTAARFITPPHLELSLGGG---GACR 145

Query: 139 RSI-----FFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDFD--TTRKSRPLDF 195
           R I      ++   A   K++          T G  S+S++SSVVDF+    + S PLD 
Sbjct: 146 RKIPLVHPVYYYNMATYPKMT----------TCGVQSESETSSVVDFEGGAGKISPPLDL 180

[Close]

BLAST of Lsi04G020490 vs. Swiss-Prot
Match: ERF4_NICSY (Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris GN=ERF4 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.0e-25
Identity = 65/115 (56.52%), Postives = 76/115 (66.09%), Query Frame = 1


Query: 20  KEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNF 79
           KE+ YRGVRKRPWGRYAAEIRDP KK+RVWLGTF+TAEEAA+AYDTAAREFRG  AKTNF
Sbjct: 23  KEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNF 82

Query: 80  PIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSDPSLTHFLDAGVHAP 135
           P+ +E               + S S  ST++SSS              + G+HAP
Sbjct: 83  PLPSE---------------NQSTSHSSTMESSSG-------------ETGIHAP 109

[Close]

BLAST of Lsi04G020490 vs. Swiss-Prot
Match: ERF4_TOBAC (Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum GN=ERF4 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.2e-24
Identity = 88/212 (41.51%), Postives = 109/212 (51.42%), Query Frame = 1


Query: 20  KEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNF 79
           KE+ YRGVRKRPWGRYAAEIRDP KK+RVWLGTF+TAEEAA+AYDTAAREFRG  AKTNF
Sbjct: 23  KEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNF 82

Query: 80  PIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSS-------PSPAPSDPSLTHFL----- 139
           P   E               + SPS  STV+SSS       P  AP +  LT  L     
Sbjct: 83  PSPTE---------------NQSPSHSSTVESSSGENGVHAPPHAPLELDLTRRLGSVAA 142

Query: 140 DAGVHA--------------PAIS-----RSIFFFDAFAQAEKISDFRRTVTVA------ 195
           D G +               P ++     + +  FD+  +A  ++  +            
Sbjct: 143 DGGDNCRRSGEVGYPIFHQQPTVAVLPNGQPVLLFDSLWRAGVVNRPQPYHVTPMGFNGV 202

[Close]

BLAST of Lsi04G020490 vs. Swiss-Prot
Match: ERF80_ARATH (Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana GN=ERF9 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.7e-23
Identity = 81/182 (44.51%), Postives = 100/182 (54.95%), Query Frame = 1


Query: 20  KEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNF 79
           KE+ +RGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAAREFRG+ AKTNF
Sbjct: 29  KEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAREFRGSKAKTNF 88

Query: 80  PIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSDPSLTHFLDAGVHAPAISRS 139
           P+  E   V++         + S    +TV ++        P   H     V   A +  
Sbjct: 89  PLPGESTTVNDGGE------NDSYVNRTTVTTAREMTRQRFPFACHRERKVVGGYASAG- 148

Query: 140 IFFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNL--PAV 199
            FFFD    A   ++  R   V      V+   S  + +       R L+ DLNL  P V
Sbjct: 149 -FFFDPSRAASLRAELSRVCPV--RFDPVNIELSIGIRETVKVEPRRELNLDLNLAPPVV 200

[Close]

BLAST of Lsi04G020490 vs. TrEMBL
Match: A0A0A0L4P1_CUCSA (Ethylene response factor 2 OS=Cucumis sativus GN=Csa_4G630010 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 4.3e-76
Identity = 164/203 (80.79%), Postives = 170/203 (83.74%), Query Frame = 1


Query: 1   MAAVDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAA DRSTST     PP SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MAAGDRSTSTT----PPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPIQNEFLFVDNNN--NFRKSACSSSPSQCSTVDSSSPSPAP 120
           RAYD AAREFRGANAKTNFP+Q +FL VDNNN  NF KSACS+SPS  STV+SSSPSP P
Sbjct: 61  RAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTP 120

Query: 121 SDPSLTHFLDAGVHAPAISRSIFFFDAFAQAEK-ISDFRRTVTVAGTGGSVSDSDSSSVV 180
           SDP    +LD G+H P IS  IFFFDAFAQAEK ISDFRRTVT A  G   SDSDSSSV 
Sbjct: 121 SDP----YLDGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAG--ASDSDSSSVG 180

Query: 181 DFDTTRKSRPLDFDLNLPAVEVV 201
           DFDTTRKSRPLDFDLNLPAVEVV
Sbjct: 181 DFDTTRKSRPLDFDLNLPAVEVV 193

[Close]

BLAST of Lsi04G020490 vs. TrEMBL
Match: M5WV14_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010982mg PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-45
Identity = 118/213 (55.40%), Postives = 138/213 (64.79%), Query Frame = 1


Query: 8   TSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAA 67
           T + S    PNSKEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD AA
Sbjct: 10  TKSGSEIPSPNSKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDKAA 69

Query: 68  REFRGANAKTNFPIQNEF-------LFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSD 127
           REFRGA AKTNFP   E        +   NNN  + +A ++SPSQ STV+SSSP P P D
Sbjct: 70  REFRGAKAKTNFPTPAELQLEAAANINSHNNNVPKAAAANNSPSQSSTVESSSPPPPPLD 129

Query: 128 PSLT-HF--------LDAGVHAPAISRSIFFFDAFAQAE---------KISDFRRTVTVA 187
            +L  HF          +G  A  ++R + FFDAFA+AE         ++  F R V   
Sbjct: 130 LALAPHFAAGGGYFTAASGFRAVPVARPVLFFDAFARAENAAALQRAREMCRFERAVVAP 189

Query: 188 GTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNLP 196
            +GG+ SDS SSSVVDFD +  +  LD DLNLP
Sbjct: 190 SSGGAHSDSSSSSVVDFDRSLPNGRLDLDLNLP 222

[Close]

BLAST of Lsi04G020490 vs. TrEMBL
Match: A0A0M4KET8_9ROSA (AP2/EREBP family transcription factor-like protein 17 OS=Prunus pseudocerasus PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.3e-45
Identity = 119/214 (55.61%), Postives = 139/214 (64.95%), Query Frame = 1


Query: 8   TSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAA 67
           T + S    PNSKEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD AA
Sbjct: 10  TKSGSEIPSPNSKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDKAA 69

Query: 68  REFRGANAKTNFPIQNEF-------LFVDNNNNFRK-SACSSSPSQCSTVDSSSPSPAPS 127
           REFRGA AKTNFP   E        +   NNNN  K +A ++SPSQ STV+SSSP P P 
Sbjct: 70  REFRGAKAKTNFPTPAELQLEAAANINSHNNNNLPKAAAANNSPSQSSTVESSSPPPPPL 129

Query: 128 DPSLT-HF--------LDAGVHAPAISRSIFFFDAFAQAE---------KISDFRRTVTV 187
           D +L  HF          +G  A  ++R + FFDAFA+AE         ++  F R V  
Sbjct: 130 DLALAPHFAAGGGYFTAASGFRAVPVARPVLFFDAFARAENAAALQRAREMCRFERAVAA 189

Query: 188 AGTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNLP 196
             +GG+ SDS SSSVV+FD +  +  LD DLNLP
Sbjct: 190 PSSGGTHSDSSSSSVVNFDRSLPNGRLDLDLNLP 223

[Close]

BLAST of Lsi04G020490 vs. TrEMBL
Match: A0A0L9V3U4_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan08g041400 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.9e-40
Identity = 107/197 (54.31%), Postives = 125/197 (63.45%), Query Frame = 1


Query: 5   DRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 64
           +RS     + +    KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD
Sbjct: 6   NRSNDVCGSNSTRLDKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYD 65

Query: 65  TAAREFRGANAKTNFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSDPSLT 124
            AAR+FRGA AKTNFP   E   V           + SPSQ ST++S SP P P D +LT
Sbjct: 66  AAARDFRGAKAKTNFPTHAELTHV-----------ARSPSQSSTLESPSP-PPPLDLTLT 125

Query: 125 HFLDAGVHAPAISRSIFFFDAFAQAE------KISDFRRTVTVAGTGGSVSDSDSSSVVD 184
                G     ++R + FFDAFA+AE       ++ FRRT   A T    SDSDSSSVVD
Sbjct: 126 PLAATGAFVFPVARPVVFFDAFARAEVRAFDLPVTAFRRTAVAATT---QSDSDSSSVVD 185

Query: 185 FDTTRKSRPLDFDLNLP 196
           ++     R LD DLN+P
Sbjct: 186 YERVPHRRVLDLDLNVP 187

[Close]

BLAST of Lsi04G020490 vs. TrEMBL
Match: A0A0S3S9Z0_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.06G056300 PE=4 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.9e-40
Identity = 107/197 (54.31%), Postives = 125/197 (63.45%), Query Frame = 1


Query: 5   DRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYD 64
           +RS     + +    KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD
Sbjct: 6   NRSNDVCGSNSTRLDKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYD 65

Query: 65  TAAREFRGANAKTNFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSDPSLT 124
            AAR+FRGA AKTNFP   E   V           + SPSQ ST++S SP P P D +LT
Sbjct: 66  AAARDFRGAKAKTNFPTHAELTHV-----------ARSPSQSSTLESPSP-PPPLDLTLT 125

Query: 125 HFLDAGVHAPAISRSIFFFDAFAQAE------KISDFRRTVTVAGTGGSVSDSDSSSVVD 184
                G     ++R + FFDAFA+AE       ++ FRRT   A T    SDSDSSSVVD
Sbjct: 126 PLAATGAFVFPVARPVVFFDAFARAEVRAFDLPVTAFRRTAVAATT---QSDSDSSSVVD 185

Query: 185 FDTTRKSRPLDFDLNLP 196
           ++     R LD DLN+P
Sbjct: 186 YERVPHRRVLDLDLNVP 187

[Close]

BLAST of Lsi04G020490 vs. TAIR10
Match: AT3G15210.1 (AT3G15210.1 ethylene responsive element binding factor 4)

HSP 1 Score: 146.7 bits (369), Expect = 1.5e-35
Identity = 103/203 (50.74%), Postives = 123/203 (60.59%), Query Frame = 1


Query: 18  NSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKT 77
           N+KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAAR+FRGA AKT
Sbjct: 19  NAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDFRGAKAKT 78

Query: 78  NFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSP--APS------DPSLTHFLDA 137
           NFP      F++ ++    +  + SPSQ ST+D +SP     PS       P L   L  
Sbjct: 79  NFPT-----FLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLELSLGG 138

Query: 138 G----VHAPAISRSIFFFDAFAQAEK--------ISDFRRTVTVAGTG---GSVSDSDSS 196
           G     +   +SR ++F D                S FR  V    T    G+ SDSDSS
Sbjct: 139 GGGGSCYQIPMSRPVYFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMACGAQSDSDSS 198

[Close]

BLAST of Lsi04G020490 vs. TAIR10
Match: AT1G53170.1 (AT1G53170.1 ethylene response factor 8)

HSP 1 Score: 119.0 bits (297), Expect = 3.3e-27
Identity = 88/184 (47.83%), Postives = 107/184 (58.15%), Query Frame = 1


Query: 19  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTN 78
           +KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TA++AARAYD AAR+FRG  AKTN
Sbjct: 26  AKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDAAARDFRGVKAKTN 85

Query: 79  FPIQNEFLFVDNNNNFRKSACSSSPSQCST-VDSSSPSPAPSDPSLTHFLDAGVHAPAIS 138
           F +                   SSP+Q ST VDS + +   + P L   L  G    A  
Sbjct: 86  FGV----------------IVGSSPTQSSTVVDSPTAARFITPPHLELSLGGG---GACR 145

Query: 139 RSI-----FFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDFD--TTRKSRPLDF 195
           R I      ++   A   K++          T G  S+S++SSVVDF+    + S PLD 
Sbjct: 146 RKIPLVHPVYYYNMATYPKMT----------TCGVQSESETSSVVDFEGGAGKISPPLDL 180

[Close]

BLAST of Lsi04G020490 vs. TAIR10
Match: AT5G44210.1 (AT5G44210.1 erf domain protein 9)

HSP 1 Score: 109.4 bits (272), Expect = 2.6e-24
Identity = 81/182 (44.51%), Postives = 100/182 (54.95%), Query Frame = 1


Query: 20  KEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTNF 79
           KE+ +RGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYDTAAREFRG+ AKTNF
Sbjct: 29  KEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAREFRGSKAKTNF 88

Query: 80  PIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPAPSDPSLTHFLDAGVHAPAISRS 139
           P+  E   V++         + S    +TV ++        P   H     V   A +  
Sbjct: 89  PLPGESTTVNDGGE------NDSYVNRTTVTTAREMTRQRFPFACHRERKVVGGYASAG- 148

Query: 140 IFFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNL--PAV 199
            FFFD    A   ++  R   V      V+   S  + +       R L+ DLNL  P V
Sbjct: 149 -FFFDPSRAASLRAELSRVCPV--RFDPVNIELSIGIRETVKVEPRRELNLDLNLAPPVV 200

[Close]

BLAST of Lsi04G020490 vs. TAIR10
Match: AT1G03800.1 (AT1G03800.1 ERF domain protein 10)

HSP 1 Score: 107.8 bits (268), Expect = 7.6e-24
Identity = 50/63 (79.37%), Postives = 56/63 (88.89%), Query Frame = 1


Query: 19  SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAAREFRGANAKTN 78
           SKE+RYRGVR+RPWGRYAAEIRDP KK RVWLG+FNT EEAARAYD+AA  FRG+ A TN
Sbjct: 48  SKEVRYRGVRRRPWGRYAAEIRDPVKKKRVWLGSFNTGEEAARAYDSAAIRFRGSKATTN 107

Query: 79  FPI 82
           FP+
Sbjct: 108 FPL 110

[Close]

BLAST of Lsi04G020490 vs. TAIR10
Match: AT1G28370.1 (AT1G28370.1 ERF domain protein 11)

HSP 1 Score: 104.8 bits (260), Expect = 6.5e-23
Identity = 87/192 (45.31%), Postives = 101/192 (52.60%), Query Frame = 1


Query: 10  TASTAAPPNSKE--IRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAA 69
           T  TAA   ++   +RYRGVRKRPWGRYAAEIRDP KK+RVWLGTF+T EEAARAYD  A
Sbjct: 4   TVKTAAVKTNEGNGVRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYDKRA 63

Query: 70  REFRGANAKTNFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPS-PAPSDPSLTHF 129
            EFRGA AKTNFP          N N    + + S SQ STV+SS P+    SD   + F
Sbjct: 64  IEFRGAKAKTNFPCY--------NINAHCLSLTQSLSQSSTVESSFPNLNLGSDSVSSRF 123

Query: 130 LDAGVHAPAISRSIFFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDFDTTR--- 189
               +   A    +  FD                        S+SDSSSVV  D  R   
Sbjct: 124 PFPKIQVKA---GMMVFD----------------------ERSESDSSSVV-MDVVRYEG 161

Query: 190 KSRPLDFDLNLP 196
           +   LD DLN P
Sbjct: 184 RRVVLDLDLNFP 161

[Close]

BLAST of Lsi04G020490 vs. NCBI nr
Match: gi|659127624|ref|XP_008463800.1| (PREDICTED: ethylene-responsive transcription factor 4 [Cucumis melo])

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-81
Identity = 170/201 (84.58%), Postives = 177/201 (88.06%), Query Frame = 1


Query: 1   MAAVDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAA DRSTS    AAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MAAGDRSTS----AAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPIQNEFLFVDNNN-NFRKSACSSSPSQCSTVDSSSPSPAPS 120
           RAYDTAAREFRGANAKTNFP+QN+FL VDNN+ NF KSACSSSPS  STV+SSSPSP PS
Sbjct: 61  RAYDTAAREFRGANAKTNFPVQNDFLIVDNNHTNFHKSACSSSPSHSSTVESSSPSPTPS 120

Query: 121 DPSLTHFLDAGVHAPAISRSIFFFDAFAQAEKISDFRRTVTVAGTGGSVSDSDSSSVVDF 180
           +P    +LD G+HAP ISR IFFFDAFAQAEKISDFRRTVT A  G   SDSDSSSVVDF
Sbjct: 121 NP----YLDGGIHAPGISRPIFFFDAFAQAEKISDFRRTVTAAAAG--ASDSDSSSVVDF 180

Query: 181 DTTRKSRPLDFDLNLPAVEVV 201
           DTTRKSRPLDFDLNLPAVEVV
Sbjct: 181 DTTRKSRPLDFDLNLPAVEVV 191

[Close]

BLAST of Lsi04G020490 vs. NCBI nr
Match: gi|449456627|ref|XP_004146050.1| (PREDICTED: ethylene-responsive transcription factor 4 [Cucumis sativus])

HSP 1 Score: 292.4 bits (747), Expect = 6.2e-76
Identity = 164/203 (80.79%), Postives = 170/203 (83.74%), Query Frame = 1


Query: 1   MAAVDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MAA DRSTST     PP SKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA
Sbjct: 1   MAAGDRSTSTT----PPTSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPIQNEFLFVDNNN--NFRKSACSSSPSQCSTVDSSSPSPAP 120
           RAYD AAREFRGANAKTNFP+Q +FL VDNNN  NF KSACS+SPS  STV+SSSPSP P
Sbjct: 61  RAYDAAAREFRGANAKTNFPLQTDFLIVDNNNHSNFHKSACSTSPSHSSTVESSSPSPTP 120

Query: 121 SDPSLTHFLDAGVHAPAISRSIFFFDAFAQAEK-ISDFRRTVTVAGTGGSVSDSDSSSVV 180
           SDP    +LD G+H P IS  IFFFDAFAQAEK ISDFRRTVT A  G   SDSDSSSV 
Sbjct: 121 SDP----YLDGGIHPPGISPPIFFFDAFAQAEKNISDFRRTVTAAVAG--ASDSDSSSVG 180

Query: 181 DFDTTRKSRPLDFDLNLPAVEVV 201
           DFDTTRKSRPLDFDLNLPAVEVV
Sbjct: 181 DFDTTRKSRPLDFDLNLPAVEVV 193

[Close]

BLAST of Lsi04G020490 vs. NCBI nr
Match: gi|645240193|ref|XP_008226493.1| (PREDICTED: ethylene-responsive transcription factor 4 [Prunus mume])

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-46
Identity = 120/205 (58.54%), Postives = 137/205 (66.83%), Query Frame = 1


Query: 8   TSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAARAYDTAA 67
           T + S    PNSKEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAARAYD AA
Sbjct: 10  TKSGSETPSPNSKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDKAA 69

Query: 68  REFRGANAKTNFPIQNEF-------LFVDNNNNFRK-SACSSSPSQCSTVDSSSPSPAPS 127
           REFRGA AKTNFP   E        +   NNNN  K +A ++SPSQ STV+SSSP P P 
Sbjct: 70  REFRGAKAKTNFPTPAELQLEAAANINSHNNNNVPKAAAANNSPSQSSTVESSSPPPPPL 129

Query: 128 DPSLTHFLDAGVHAPAISRSIFFFDAFAQAE---------KISDFRRTVTVAGTGGSVSD 187
           D SL     AG  A  ++R + FFDAFA+AE         ++  F R V    +GG+ SD
Sbjct: 130 DLSLAPHFAAG--AVPVARPVLFFDAFARAENAAALQRAREMCRFERAVAAPSSGGAHSD 189

Query: 188 SDSSSVVDFDTTRKSRPLDFDLNLP 196
           S SSSVVDFD +  +  LD DLNLP
Sbjct: 190 SSSSSVVDFDRSLPNGRLDLDLNLP 212

[Close]

BLAST of Lsi04G020490 vs. NCBI nr
Match: gi|1009161867|ref|XP_015899126.1| (PREDICTED: ethylene-responsive transcription factor 4-like [Ziziphus jujuba])

HSP 1 Score: 193.7 bits (491), Expect = 3.0e-46
Identity = 127/215 (59.07%), Postives = 142/215 (66.05%), Query Frame = 1


Query: 1   MAAVDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MA  DRST+       PN KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAA
Sbjct: 1   MAPRDRSTAVNPD---PNCKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPA--- 120
           RAYDTAAREFRGA AKTNFP   E      NN    +  + SPSQ STV+SSSP P    
Sbjct: 61  RAYDTAAREFRGAKAKTNFPTPAELHSF--NNTHSNNTPTRSPSQSSTVESSSPPPPIST 120

Query: 121 ----PSDPSLT-HFLDA-------GVHAPAISRSIFFFDAFAQAEKISDFRRTV-----T 180
               P D SLT HF          G HA  ++R +FFFDAFA+AE ++  RR +     T
Sbjct: 121 SPPPPLDLSLTPHFTGGYFAAAPGGFHALPVARPVFFFDAFARAETMAGHRREMCRFDRT 180

Query: 181 VAGTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNLP 196
           VAG G   SDS+SSSVVDF+    +RPLD DLNLP
Sbjct: 181 VAG-GAVHSDSNSSSVVDFENGLTNRPLDLDLNLP 209

[Close]

BLAST of Lsi04G020490 vs. NCBI nr
Match: gi|1009162061|ref|XP_015899231.1| (PREDICTED: ethylene-responsive transcription factor 4-like [Ziziphus jujuba])

HSP 1 Score: 193.7 bits (491), Expect = 3.0e-46
Identity = 127/215 (59.07%), Postives = 142/215 (66.05%), Query Frame = 1


Query: 1   MAAVDRSTSTASTAAPPNSKEIRYRGVRKRPWGRYAAEIRDPRKKTRVWLGTFNTAEEAA 60
           MA  DRST+       PN KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTF+TAEEAA
Sbjct: 1   MAPRDRSTAVNPD---PNCKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 60

Query: 61  RAYDTAAREFRGANAKTNFPIQNEFLFVDNNNNFRKSACSSSPSQCSTVDSSSPSPA--- 120
           RAYDTAAREFRGA AKTNFP   E      NN    +  + SPSQ STV+SSSP P    
Sbjct: 61  RAYDTAAREFRGAKAKTNFPTPAELHSF--NNTHSNNTPTRSPSQSSTVESSSPPPPIST 120

Query: 121 ----PSDPSLT-HFLDA-------GVHAPAISRSIFFFDAFAQAEKISDFRRTV-----T 180
               P D SLT HF          G HA  ++R +FFFDAFA+AE ++  RR +     T
Sbjct: 121 SPPPPLDLSLTPHFTGGYFAAAPGGFHALPVARPVFFFDAFARAETMTGHRREMCRFDRT 180

Query: 181 VAGTGGSVSDSDSSSVVDFDTTRKSRPLDFDLNLP 196
           VAG G   SDS+SSSVVDF+    +RPLD DLNLP
Sbjct: 181 VAG-GAVHSDSNSSSVVDFENGLTNRPLDLDLNLP 209

The following BLAST results are available for this feature:

BLAST of Lsi04G020490 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ERF78_ARATH	2.6e-34	50.74	Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana GN=ERF4 PE=1 ...	[more]
ERF79_ARATH	5.9e-26	47.83	Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana GN=ERF8 PE=2 ...	[more]
ERF4_NICSY	5.0e-25	56.52	Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris GN=ERF4 PE=2 ...	[more]
ERF4_TOBAC	3.2e-24	41.51	Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum GN=ERF4 PE=1 SV=...	[more]
ERF80_ARATH	4.7e-23	44.51	Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana GN=ERF9 PE=2 ...	[more]

BLAST of Lsi04G020490 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4P1_CUCSA	4.3e-76	80.79	Ethylene response factor 2 OS=Cucumis sativus GN=Csa_4G630010 PE=4 SV=1	[more]
M5WV14_PRUPE	1.4e-45	55.40	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010982mg PE=4 SV=1	[more]
A0A0M4KET8_9ROSA	2.3e-45	55.61	AP2/EREBP family transcription factor-like protein 17 OS=Prunus pseudocerasus PE...	[more]
A0A0L9V3U4_PHAAN	2.9e-40	54.31	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan08g041400 PE=4 SV=1	[more]
A0A0S3S9Z0_PHAAN	2.9e-40	54.31	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.06G056300 PE=...	[more]

BLAST of Lsi04G020490 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G15210.1	1.5e-35	50.74	ethylene responsive element binding factor 4	[more]
AT1G53170.1	3.3e-27	47.83	ethylene response factor 8	[more]
AT5G44210.1	2.6e-24	44.51	erf domain protein 9	[more]
AT1G03800.1	7.6e-24	79.37	ERF domain protein 10	[more]
AT1G28370.1	6.5e-23	45.31	ERF domain protein 11	[more]

BLAST of Lsi04G020490 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659127624\|ref\|XP_008463800.1\|	1.3e-81	84.58	PREDICTED: ethylene-responsive transcription factor 4 [Cucumis melo]	[more]
gi\|449456627\|ref\|XP_004146050.1\|	6.2e-76	80.79	PREDICTED: ethylene-responsive transcription factor 4 [Cucumis sativus]	[more]
gi\|645240193\|ref\|XP_008226493.1\|	1.0e-46	58.54	PREDICTED: ethylene-responsive transcription factor 4 [Prunus mume]	[more]
gi\|1009161867\|ref\|XP_015899126.1\|	3.0e-46	59.07	PREDICTED: ethylene-responsive transcription factor 4-like [Ziziphus jujuba]	[more]
gi\|1009162061\|ref\|XP_015899231.1\|	3.0e-46	59.07	PREDICTED: ethylene-responsive transcription factor 4-like [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR016177	DNA-bd_dom_sf
IPR001471	AP2/ERF_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0071229	cellular response to acid chemical
biological_process	GO:0071310	cellular response to organic substance
biological_process	GO:1901701	cellular response to oxygen-containing compound
biological_process	GO:0006952	defense response
biological_process	GO:0009725	response to hormone
biological_process	GO:0007165	signal transduction
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G020490.1	Lsi04G020490.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 46..62 score: 1.8E-11coord: 24..35 score: 1.8
IPR001471	AP2/ERF domain	GENE3D	G3DSA:3.30.730.10		coord: 23..81 score: 2.2
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 23..72 score: 2.1
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 23..86 score: 2.4
IPR001471	AP2/ERF domain	PROFILE	PS51032	AP2_ERF	coord: 23..80 score: 2
IPR016177	DNA-binding domain	unknown	SSF54171	DNA-binding domain	coord: 23..82 score: 6.54
None	No IPR available	PANTHER	PTHR31677	FAMILY NOT NAMED	coord: 18..115 score: 3.0
None	No IPR available	PANTHER	PTHR31677:SF17	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 10-RELATED	coord: 18..115 score: 3.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G020490	Csa4G630010	Cucumber (Chinese Long) v2	culsiB283
Lsi04G020490	CSPI04G23250	Wild cucumber (PI 183967)	cpilsiB283
Lsi04G020490	Cucsa.122930	Cucumber (Gy14) v1	cgylsiB185
Lsi04G020490	CmaCh11G019100	Cucurbita maxima (Rimu)	cmalsiB122
Lsi04G020490	CmaCh01G012220	Cucurbita maxima (Rimu)	cmalsiB432
Lsi04G020490	CmaCh09G008460	Cucurbita maxima (Rimu)	cmalsiB023
Lsi04G020490	CmoCh09G008400	Cucurbita moschata (Rifu)	cmolsiB011
Lsi04G020490	CmoCh11G019920	Cucurbita moschata (Rifu)	cmolsiB110
Lsi04G020490	CmoCh01G012750	Cucurbita moschata (Rifu)	cmolsiB421
Lsi04G020490	Cla002237	Watermelon (97103) v1	lsiwmB310
Lsi04G020490	Cla023361	Watermelon (97103) v1	lsiwmB333
Lsi04G020490	MELO3C025608	Melon (DHL92) v3.5.1	lsimeB315
Lsi04G020490	ClCG11G017830	Watermelon (Charleston Gray)	lsiwcgB259
Lsi04G020490	ClCG07G002550	Watermelon (Charleston Gray)	lsiwcgB299
Lsi04G020490	MELO3C025608.2	Melon (DHL92) v3.6.1	lsimedB331
Lsi04G020490	Cla97C11G223860	Watermelon (97103) v2	lsiwmbB266
Lsi04G020490	Bhi09G002830	Wax gourd	lsiwgoB402
Lsi04G020490	CsGy4G021630	Cucumber (Gy14) v2	cgyblsiB263
Lsi04G020490	Carg14708	Silver-seed gourd	carlsiB286
Lsi04G020490	Carg17081	Silver-seed gourd	carlsiB151
Lsi04G020490	CsaV3_4G033470	Cucumber (Chinese Long) v3	cuclsiB304

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Lsi04G020490	Lsi02G007760	Bottle gourd (USVL1VR-Ls)	lsilsiB105

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G020490	Cucurbita maxima (Rimu)	cmalsiB311
Lsi04G020490	Cucurbita maxima (Rimu)	cmalsiB439
Lsi04G020490	Cucurbita moschata (Rifu)	cmolsiB299
Lsi04G020490	Cucurbita pepo (Zucchini)	cpelsiB456
Lsi04G020490	Cucurbita pepo (Zucchini)	cpelsiB637