Lsi04G011260 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G011260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Calcium-dependent lipid-binding domain-containing protein
Location	chr04 : 14816237 .. 14817954 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTAAGTGAATTTGTCAAAGGAAATGAATGCTGAATATGTCAATAGACGGGAATATTACTTGCAATTTTATAATCCAACAAAAATGGGTCAATCTTCTTTCTTCTTCTTCCTCGTCGATTCCTTGTTCTTATATAATTCTCATCTCCATAACTTCAATGATCAAATTCAAGATTATGCGAAGACTTTGAAATGAACAATCGGGCTGGACTCCTTCGAATTCGCCTGCTTCGAGGCCACAATCTCGCCATTCGCGATGCTCCAACTCGTAGCAGCGATCCCTACGTCGTCATCTCCTGCGCCAACCAGGTTTCTTTTTCCCTTCGTTGCTTACGATTTACTCAGAAATTGTTTATGTGTTCATGTGTGAAACGGTTCGGATTTGGATTGCGATCTTTTCTCTGAATTTCCTTCGAATTATCTTGTCAAACTTTTCCGATTTTAGGTTTTCTGTTCGGATTTCATGTCGAAACTCGCTGAACTGTTCGAATTCGGATCGCGATCTTCCTCCTTCGAAATATTAGTTGAATTTTCCCGTTCTTAAGTTTCTCTGTTTGGACTTTGGATTTTGGATTGCAATTTCCTTTATCAGATCTTCGTTCGAAATATTAGTCTAGTTTCTGCGTTTTTGGTTTTTTCTGTTTGGATTTCTGATTCAAGTTTCTCTACAACTTCCTTGCAAATATTTGTTGAATTTTTCGGTTTCTAAGTTCTCTATCCGGATTTCCCCTCTCCTAACTTTATGCAAACGGCTCGAATTCCAACTTCTTGCAAATATTGGTCAAACTCTTCCGTTTTTAGGTTTTTCCGTTCGAAACTATGCAAACGGAAACGATTCAAATTCTGACTGAAATTTCTCCATTGGAAGATCTCGGATTTGTTGATCTCTTGGTCTGACTCTTCTCGGATGCAAACCTTTTGTGCAGAAATTCAAATCTCGTGTGGTGAAAAAAAACTGCAATCCAGAATGGAACGAGGAATTCACACTTTCGATCACAGATATCAACACGCCAATCAAATTAGTAAGTCAAAATTGAACAATAATATTTGAAACTTGAGATGAATGAAACATCCATCATTCAATAAAAGTAATTCTATGAACTGTGTATCTCAGGCAGTGTTCGACAAAGATAGATTCACAAAGGACGATGGAATGGGGGATGCAGAGATCGACATAAAACCATACATAGAGTGTTTGAATATGGGATTAGAGAATCTTCCAAATGGTTGTGTGGTGAAAAGGGTTCAACCAAGCAGATCAAACAGCCTTGCAGATGAGAGCCCTTGTGTTTGGAACGATGGTAAGATTATCCAAGACATGACTCTCAGATTGCGGAATGTTGAATGTGGTGAAATCATGATCCAGCTTCAGTTGTTCAATGTTTCTATTTTCAAAGGCCTAAGAACCTACTCTTTAGCCTGAACAAACTTTGCTCCTGCCTTTGCTTTTGCTTTTGTGATTGGTTCTTCGTTGCCGTTCTTCGTCTCGCCATCGTTCTGCTTCTTCTCTGTTTTCTCATCAACACCAACTCCAGAATTTAACCATTTAATTTGCTTGTAAATGTAGATTGTCATTTTGTTGGTATGATATTGTGAAATGGTATTGTACTATTGTTTGAGTACTTAGAACTATTCTTATTTTTTACTCTCAAGGTTCTTGGTTTATTTGAATGCTTTGGTTGAGGTGGTTGTTCATTTTTTTCTTTGTTATATTTTTGCA

mRNA sequence

CGTAAGTGAATTTGTCAAAGGAAATGAATGCTGAATATGTCAATAGACGGGAATATTACTTGCAATTTTATAATCCAACAAAAATGGGTCAATCTTCTTTCTTCTTCTTCCTCGTCGATTCCTTGTTCTTATATAATTCTCATCTCCATAACTTCAATGATCAAATTCAAGATTATGCGAAGACTTTGAAATGAACAATCGGGCTGGACTCCTTCGAATTCGCCTGCTTCGAGGCCACAATCTCGCCATTCGCGATGCTCCAACTCGTAGCAGCGATCCCTACGTCGTCATCTCCTGCGCCAACCAGAAATTCAAATCTCGTGTGGTGAAAAAAAACTGCAATCCAGAATGGAACGAGGAATTCACACTTTCGATCACAGATATCAACACGCCAATCAAATTAGCAGTGTTCGACAAAGATAGATTCACAAAGGACGATGGAATGGGGGATGCAGAGATCGACATAAAACCATACATAGAGTGTTTGAATATGGGATTAGAGAATCTTCCAAATGGTTGTGTGGTGAAAAGGGTTCAACCAAGCAGATCAAACAGCCTTGCAGATGAGAGCCCTTGTGTTTGGAACGATGGTAAGATTATCCAAGACATGACTCTCAGATTGCGGAATGTTGAATGTGGTGAAATCATGATCCAGCTTCAGTTGTTCAATGTTTCTATTTTCAAAGGCCTAAGAACCTACTCTTTAGCCTGAACAAACTTTGCTCCTGCCTTTGCTTTTGCTTTTGTGATTGGTTCTTCGTTGCCGTTCTTCGTCTCGCCATCGTTCTGCTTCTTCTCTGTTTTCTCATCAACACCAACTCCAGAATTTAACCATTTAATTTGCTTGTAAATGTAGATTGTCATTTTGTTGGTATGATATTGTGAAATGGTATTGTACTATTGTTTGAGTACTTAGAACTATTCTTATTTTTTACTCTCAAGGTTCTTGGTTTATTTGAATGCTTTGGTTGAGGTGGTTGTTCATTTTTTTCTTTGTTATATTTTTGCA

Coding sequence (CDS)

ATGAACAATCGGGCTGGACTCCTTCGAATTCGCCTGCTTCGAGGCCACAATCTCGCCATTCGCGATGCTCCAACTCGTAGCAGCGATCCCTACGTCGTCATCTCCTGCGCCAACCAGAAATTCAAATCTCGTGTGGTGAAAAAAAACTGCAATCCAGAATGGAACGAGGAATTCACACTTTCGATCACAGATATCAACACGCCAATCAAATTAGCAGTGTTCGACAAAGATAGATTCACAAAGGACGATGGAATGGGGGATGCAGAGATCGACATAAAACCATACATAGAGTGTTTGAATATGGGATTAGAGAATCTTCCAAATGGTTGTGTGGTGAAAAGGGTTCAACCAAGCAGATCAAACAGCCTTGCAGATGAGAGCCCTTGTGTTTGGAACGATGGTAAGATTATCCAAGACATGACTCTCAGATTGCGGAATGTTGAATGTGGTGAAATCATGATCCAGCTTCAGTTGTTCAATGTTTCTATTTTCAAAGGCCTAAGAACCTACTCTTTAGCCTGA

Protein sequence

MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLSITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSNSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGLRTYSLA

Homology

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BLAST of Lsi04G011260 vs. Swiss-Prot
Match: CAR10_ARATH (Protein C2-DOMAIN ABA-RELATED 10 OS=Arabidopsis thaliana GN=CAR10 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.4e-52
Identity = 103/168 (61.31%), Postives = 125/168 (74.40%), Query Frame = 1


Query: 6   GLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLSITDI 65
           GLL I + RG NLAIRD   RSSDPY+V++ A+Q  K+RVVKKNCNP WNEE T++I D 
Sbjct: 7   GLLTIHVKRGINLAIRDH--RSSDPYIVLNVADQTLKTRVVKKNCNPVWNEEMTVAIKDP 66

Query: 66  NTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLE--NLPNGCVVKRVQPSRSNSL 125
           N PI+L VFD D+FT DD MGDA IDI+PY+E L MG+E   LPNGC +KRVQPSR N L
Sbjct: 67  NVPIRLTVFDWDKFTGDDKMGDANIDIQPYLEALKMGMELLRLPNGCAIKRVQPSRHNCL 126

Query: 126 ADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGLRTYS 172
           +DES  VWN+GKI QD+ LRL NVECGEI I L+    +  +G+ + S
Sbjct: 127 SDESSIVWNNGKITQDLILRLNNVECGEIEIMLEWHEGAGCRGITSSS 172

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BLAST of Lsi04G011260 vs. Swiss-Prot
Match: CAR9_ARATH (Protein C2-DOMAIN ABA-RELATED 9 OS=Arabidopsis thaliana GN=CAR9 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 7.8e-51
Identity = 96/164 (58.54%), Postives = 124/164 (75.61%), Query Frame = 1


Query: 6   GLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLSITDI 65
           G+LR+ + RG NLAIRDA T  SDPYVVI+ ANQK K+RV+  NCNP WNE+ TLSI D+
Sbjct: 7   GILRVHVKRGINLAIRDATT--SDPYVVITLANQKLKTRVINNNCNPVWNEQLTLSIKDV 66

Query: 66  NTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGL--ENLPNGCVVKRVQPSRSNSL 125
           N PI+L VFDKDRF+ DD MGDAEID +P++E   M L  + LPNGC +KR++P R+N L
Sbjct: 67  NDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEAHQMELDFQKLPNGCAIKRIRPGRTNCL 126

Query: 126 ADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           A+ES   W++GKI+Q+M LRL+NVECGE+ + L+  +    KGL
Sbjct: 127 AEESSITWSNGKIMQEMILRLKNVECGEVELMLEWTDGPGCKGL 168

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BLAST of Lsi04G011260 vs. Swiss-Prot
Match: CAR4_ARATH (Protein C2-DOMAIN ABA-RELATED 4 OS=Arabidopsis thaliana GN=CAR4 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 3.3e-49
Identity = 95/167 (56.89%), Postives = 121/167 (72.46%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M++  GLLRIR+ RG NLA+RD    SSDPYVV+    QK K+RV+ K+ NPEWNE+ TL
Sbjct: 13  MDDLLGLLRIRIKRGVNLAVRDIS--SSDPYVVVKMGKQKLKTRVINKDVNPEWNEDLTL 72

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           S+TD N  + L V+D D F+KDD MGDAE +IKPYIE L M L+ LP+G +V  V+PSR 
Sbjct: 73  SVTDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEALRMQLDGLPSGTIVTTVKPSRR 132

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LA+ES   W DGK++QD+ LRLR+VECGE+  QLQ  ++   KGL
Sbjct: 133 NCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQWIDLPGSKGL 177

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BLAST of Lsi04G011260 vs. Swiss-Prot
Match: CAR5_ARATH (Protein C2-DOMAIN ABA-RELATED 5 OS=Arabidopsis thaliana GN=CAR5 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.2e-48
Identity = 92/157 (58.60%), Postives = 117/157 (74.52%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M++  GLLRIR+ RG NLA+RD    SSDPYVV+  A QK K+RV+ KN NPEWNE+ TL
Sbjct: 37  MDSLLGLLRIRIKRGVNLAVRDL--NSSDPYVVVKMAKQKLKTRVIYKNVNPEWNEDLTL 96

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           S++D N  + L V+D D FTKDD MGDAE  IKP++  L M L +LP+G +V  VQPSR 
Sbjct: 97  SVSDPNLTVLLTVYDYDTFTKDDKMGDAEFGIKPFVNALKMHLHDLPSGTIVTTVQPSRD 156

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQ 158
           N LA+ES  +W+DGK++QD+ LRLR+VECGE+  QLQ
Sbjct: 157 NCLAEESRVIWSDGKLVQDIVLRLRHVECGEVEAQLQ 191

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BLAST of Lsi04G011260 vs. Swiss-Prot
Match: CAR7_ARATH (Protein C2-DOMAIN ABA-RELATED 7 OS=Arabidopsis thaliana GN=CAR7 PE=1 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 6.8e-47
Identity = 94/167 (56.29%), Postives = 121/167 (72.46%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M    GLLRIR+ RG NLA RD  T SSDP+VVI+  +QK K+RVV+ NCNPEWNEE TL
Sbjct: 1   MEELVGLLRIRVKRGINLAQRD--TLSSDPFVVITMGSQKLKTRVVENNCNPEWNEELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           ++   + P+ L V+DKD FT  D MGDA+IDIKP++E   MGL+ LP+G  +KRV P+R 
Sbjct: 61  ALRHPDEPVNLIVYDKDTFTSHDKMGDAKIDIKPFLEVHKMGLQELPDGTEIKRVVPNRE 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LA+ S  V N+GKI+Q+M L LRNVECGE+ IQL+  ++   +GL
Sbjct: 121 NCLAEASSIVSNNGKIVQNMILLLRNVECGEVEIQLEWIDIPGSRGL 165

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BLAST of Lsi04G011260 vs. TrEMBL
Match: A0A0A0KJA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G030450 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 1.6e-90
Identity = 161/172 (93.60%), Postives = 170/172 (98.84%), Query Frame = 1


Query: 2   NNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLS 61
           +NR+GLLRIRLLRGHNLAIRDAPTRSSDPYVVI+ ANQKFKSRVVKKNCNPEWNEEFTLS
Sbjct: 4   DNRSGLLRIRLLRGHNLAIRDAPTRSSDPYVVITSANQKFKSRVVKKNCNPEWNEEFTLS 63

Query: 62  ITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSN 121
           +TD+NTPIKLAVFDKDRFTKDDGMGDAEIDIKPY+ECLNMGLENLPNGCVVKRVQPSRSN
Sbjct: 64  VTDVNTPIKLAVFDKDRFTKDDGMGDAEIDIKPYMECLNMGLENLPNGCVVKRVQPSRSN 123

Query: 122 SLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGLRTYSLA 174
           SLADESPCVWNDGKI+QDMTLRL+NVECGEIMIQLQLFNVSIF+G RTYSLA
Sbjct: 124 SLADESPCVWNDGKIVQDMTLRLQNVECGEIMIQLQLFNVSIFRGRRTYSLA 175

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BLAST of Lsi04G011260 vs. TrEMBL
Match: B9HW51_POPTR (C2 domain-containing family protein OS=Populus trichocarpa GN=POPTR_0010s12100g PE=4 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 5.4e-59
Identity = 115/167 (68.86%), Postives = 134/167 (80.24%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M+N  GLLRIR+ RG+NLA+RD  T  SDPY VI+   QK K+RVVKKNCNPEWNEE TL
Sbjct: 1   MDNLLGLLRIRVKRGNNLAVRDLGT--SDPYAVITMGKQKLKTRVVKKNCNPEWNEELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           SITD+N PI L VFDKDRFT DD MG+AEIDIK YI  L MGL+NLPNGCVV R++PSR+
Sbjct: 61  SITDLNVPINLTVFDKDRFTVDDKMGEAEIDIKAYIASLKMGLQNLPNGCVVSRIKPSRN 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LADES  VW++GKI+QDM LRLRNVE GE+MIQ++  NV   +GL
Sbjct: 121 NCLADESCVVWDNGKILQDMILRLRNVESGEVMIQIEWMNVPGCRGL 165

[Close]

BLAST of Lsi04G011260 vs. TrEMBL
Match: A5BXZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g05230 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.5e-58
Identity = 111/167 (66.47%), Postives = 130/167 (77.84%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M N  GLLR+R+ RG NLA+RDA  RSSDPYV ++   QK K+RVVK NCNPEWNEE TL
Sbjct: 1   MENLLGLLRLRVRRGINLAVRDA--RSSDPYVAVTMGEQKLKTRVVKDNCNPEWNEELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           SI D + PI L V+D D FT DD MGDAEIDIKPY+ECL MGLENLP G V+ RVQPSR+
Sbjct: 61  SIADTDVPINLVVYDSDTFTLDDKMGDAEIDIKPYVECLKMGLENLPTGTVISRVQPSRT 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LADES CVW++GKI QDM LRLRNVECGE+ +Q++  N+   +GL
Sbjct: 121 NCLADESCCVWDNGKIRQDMLLRLRNVECGEVEVQIEWINIPGCRGL 165

[Close]

BLAST of Lsi04G011260 vs. TrEMBL
Match: A0A0A0KRJ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G569360 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 5.1e-57
Identity = 108/167 (64.67%), Postives = 133/167 (79.64%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M +  GLLRIR+LRG NLAIRD  TRSSDPYV+++  +Q+ K+RVVK NCNPEWN+E TL
Sbjct: 1   MEHFLGLLRIRVLRGVNLAIRD--TRSSDPYVLVTMGDQRLKTRVVKNNCNPEWNDELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           S+ D+ TPI L+V+DKD  T  D MGDAEIDI PYIECL MGL++LP+GCVV+R+ PSR+
Sbjct: 61  SVADLRTPIGLSVYDKDTLTDHDKMGDAEIDIGPYIECLRMGLQSLPDGCVVRRLYPSRT 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LADES CVW  GKI+Q+M LRL+N ECGE+ +QL+  NV   KGL
Sbjct: 121 NCLADESQCVWQKGKIVQNMILRLKNAECGEVAVQLEWINVPGAKGL 165

[Close]

BLAST of Lsi04G011260 vs. TrEMBL
Match: W9QZY4_9ROSA (ADP-ribosylation factor GTPase-activating protein AGD12 OS=Morus notabilis GN=L484_002690 PE=4 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 9.6e-56
Identity = 109/163 (66.87%), Postives = 128/163 (78.53%), Query Frame = 1


Query: 7   LLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQ--KFKSRVVKKNCNPEWNEEFTLSITD 66
           LLRIR+ RG NLA+RD  T SSDPYV+++   Q  K K+RVVK NCNPEWN+E TLSITD
Sbjct: 5   LLRIRVRRGVNLAVRD--TLSSDPYVLVTMGEQACKLKTRVVKNNCNPEWNDELTLSITD 64

Query: 67  INTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSNSLA 126
           + TPI L VFD+D  T DD MGDAEIDIKPYIECL MGLEN P+G VVKR+QPS  N L+
Sbjct: 65  LETPITLTVFDRDTLTSDDKMGDAEIDIKPYIECLKMGLENRPDGSVVKRIQPSHENCLS 124

Query: 127 DESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           DES CVW +GKI+QDM LRLRNVECG+I++Q++  N    KGL
Sbjct: 125 DESSCVWTNGKIVQDMCLRLRNVECGKIVVQIEWINFPGCKGL 165

[Close]

BLAST of Lsi04G011260 vs. TAIR10
Match: AT2G01540.1 (AT2G01540.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 206.5 bits (524), Expect = 1.4e-53
Identity = 103/168 (61.31%), Postives = 125/168 (74.40%), Query Frame = 1


Query: 6   GLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLSITDI 65
           GLL I + RG NLAIRD   RSSDPY+V++ A+Q  K+RVVKKNCNP WNEE T++I D 
Sbjct: 7   GLLTIHVKRGINLAIRDH--RSSDPYIVLNVADQTLKTRVVKKNCNPVWNEEMTVAIKDP 66

Query: 66  NTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLE--NLPNGCVVKRVQPSRSNSL 125
           N PI+L VFD D+FT DD MGDA IDI+PY+E L MG+E   LPNGC +KRVQPSR N L
Sbjct: 67  NVPIRLTVFDWDKFTGDDKMGDANIDIQPYLEALKMGMELLRLPNGCAIKRVQPSRHNCL 126

Query: 126 ADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGLRTYS 172
           +DES  VWN+GKI QD+ LRL NVECGEI I L+    +  +G+ + S
Sbjct: 127 SDESSIVWNNGKITQDLILRLNNVECGEIEIMLEWHEGAGCRGITSSS 172

[Close]

BLAST of Lsi04G011260 vs. TAIR10
Match: AT1G70790.2 (AT1G70790.2 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 201.4 bits (511), Expect = 4.4e-52
Identity = 96/164 (58.54%), Postives = 124/164 (75.61%), Query Frame = 1


Query: 6   GLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLSITDI 65
           G+LR+ + RG NLAIRDA T  SDPYVVI+ ANQK K+RV+  NCNP WNE+ TLSI D+
Sbjct: 7   GILRVHVKRGINLAIRDATT--SDPYVVITLANQKLKTRVINNNCNPVWNEQLTLSIKDV 66

Query: 66  NTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGL--ENLPNGCVVKRVQPSRSNSL 125
           N PI+L VFDKDRF+ DD MGDAEID +P++E   M L  + LPNGC +KR++P R+N L
Sbjct: 67  NDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEAHQMELDFQKLPNGCAIKRIRPGRTNCL 126

Query: 126 ADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           A+ES   W++GKI+Q+M LRL+NVECGE+ + L+  +    KGL
Sbjct: 127 AEESSITWSNGKIMQEMILRLKNVECGEVELMLEWTDGPGCKGL 168

[Close]

BLAST of Lsi04G011260 vs. TAIR10
Match: AT3G17980.1 (AT3G17980.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 196.1 bits (497), Expect = 1.8e-50
Identity = 95/167 (56.89%), Postives = 121/167 (72.46%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M++  GLLRIR+ RG NLA+RD    SSDPYVV+    QK K+RV+ K+ NPEWNE+ TL
Sbjct: 13  MDDLLGLLRIRIKRGVNLAVRDIS--SSDPYVVVKMGKQKLKTRVINKDVNPEWNEDLTL 72

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           S+TD N  + L V+D D F+KDD MGDAE +IKPYIE L M L+ LP+G +V  V+PSR 
Sbjct: 73  SVTDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEALRMQLDGLPSGTIVTTVKPSRR 132

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LA+ES   W DGK++QD+ LRLR+VECGE+  QLQ  ++   KGL
Sbjct: 133 NCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQWIDLPGSKGL 177

[Close]

BLAST of Lsi04G011260 vs. TAIR10
Match: AT1G48590.2 (AT1G48590.2 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 194.1 bits (492), Expect = 7.0e-50
Identity = 92/157 (58.60%), Postives = 117/157 (74.52%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M++  GLLRIR+ RG NLA+RD    SSDPYVV+  A QK K+RV+ KN NPEWNE+ TL
Sbjct: 37  MDSLLGLLRIRIKRGVNLAVRDL--NSSDPYVVVKMAKQKLKTRVIYKNVNPEWNEDLTL 96

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           S++D N  + L V+D D FTKDD MGDAE  IKP++  L M L +LP+G +V  VQPSR 
Sbjct: 97  SVSDPNLTVLLTVYDYDTFTKDDKMGDAEFGIKPFVNALKMHLHDLPSGTIVTTVQPSRD 156

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQ 158
           N LA+ES  +W+DGK++QD+ LRLR+VECGE+  QLQ
Sbjct: 157 NCLAEESRVIWSDGKLVQDIVLRLRHVECGEVEAQLQ 191

[Close]

BLAST of Lsi04G011260 vs. TAIR10
Match: AT1G70810.1 (AT1G70810.1 Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 188.3 bits (477), Expect = 3.8e-48
Identity = 94/167 (56.29%), Postives = 121/167 (72.46%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M    GLLRIR+ RG NLA RD  T SSDP+VVI+  +QK K+RVV+ NCNPEWNEE TL
Sbjct: 1   MEELVGLLRIRVKRGINLAQRD--TLSSDPFVVITMGSQKLKTRVVENNCNPEWNEELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           ++   + P+ L V+DKD FT  D MGDA+IDIKP++E   MGL+ LP+G  +KRV P+R 
Sbjct: 61  ALRHPDEPVNLIVYDKDTFTSHDKMGDAKIDIKPFLEVHKMGLQELPDGTEIKRVVPNRE 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LA+ S  V N+GKI+Q+M L LRNVECGE+ IQL+  ++   +GL
Sbjct: 121 NCLAEASSIVSNNGKIVQNMILLLRNVECGEVEIQLEWIDIPGSRGL 165

[Close]

BLAST of Lsi04G011260 vs. NCBI nr
Match: gi|449449040|ref|XP_004142273.1| (PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cucumis sativus])

HSP 1 Score: 340.1 bits (871), Expect = 2.2e-90
Identity = 161/172 (93.60%), Postives = 170/172 (98.84%), Query Frame = 1


Query: 2   NNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLS 61
           +NR+GLLRIRLLRGHNLAIRDAPTRSSDPYVVI+ ANQKFKSRVVKKNCNPEWNEEFTLS
Sbjct: 4   DNRSGLLRIRLLRGHNLAIRDAPTRSSDPYVVITSANQKFKSRVVKKNCNPEWNEEFTLS 63

Query: 62  ITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSN 121
           +TD+NTPIKLAVFDKDRFTKDDGMGDAEIDIKPY+ECLNMGLENLPNGCVVKRVQPSRSN
Sbjct: 64  VTDVNTPIKLAVFDKDRFTKDDGMGDAEIDIKPYMECLNMGLENLPNGCVVKRVQPSRSN 123

Query: 122 SLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGLRTYSLA 174
           SLADESPCVWNDGKI+QDMTLRL+NVECGEIMIQLQLFNVSIF+G RTYSLA
Sbjct: 124 SLADESPCVWNDGKIVQDMTLRLQNVECGEIMIQLQLFNVSIFRGRRTYSLA 175

[Close]

BLAST of Lsi04G011260 vs. NCBI nr
Match: gi|659129460|ref|XP_008464698.1| (PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cucumis melo])

HSP 1 Score: 338.6 bits (867), Expect = 6.5e-90
Identity = 161/171 (94.15%), Postives = 167/171 (97.66%), Query Frame = 1


Query: 3   NRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTLSI 62
           NR GLLRIRLLRGHNLAIRDAPTRSSDPYVVI+CANQKFKSRVVK+NCNPEWNEEFT S+
Sbjct: 5   NRNGLLRIRLLRGHNLAIRDAPTRSSDPYVVITCANQKFKSRVVKRNCNPEWNEEFTFSL 64

Query: 63  TDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSNS 122
           TDINTPIKLAVFD+DRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSNS
Sbjct: 65  TDINTPIKLAVFDRDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRSNS 124

Query: 123 LADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGLRTYSLA 174
           LADESPCVWNDGKI QDMTLRL+NVECGEIMIQ+QLFNVSIFKG RTYSLA
Sbjct: 125 LADESPCVWNDGKITQDMTLRLQNVECGEIMIQVQLFNVSIFKGRRTYSLA 175

[Close]

BLAST of Lsi04G011260 vs. NCBI nr
Match: gi|1009154994|ref|XP_015895476.1| (PREDICTED: protein C2-DOMAIN ABA-RELATED 7-like [Ziziphus jujuba])

HSP 1 Score: 245.4 bits (625), Expect = 7.5e-62
Identity = 116/167 (69.46%), Postives = 140/167 (83.83%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M N  GLLRIR+ RG NLA+RD  TRSSDPYVVI+  NQ+ KSRV+KKNCNPEWN+E TL
Sbjct: 1   MENTLGLLRIRVQRGINLAVRD--TRSSDPYVVITMGNQRLKSRVIKKNCNPEWNDELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           SI+D++TPI L V+DKD  T DD MG+AEIDIKP IECL MGLE LPNGCVVKRVQP+R+
Sbjct: 61  SISDLDTPITLTVYDKDTLTVDDKMGEAEIDIKPLIECLRMGLEKLPNGCVVKRVQPNRT 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LADES CVWN+GKI+Q+M+LRL+NVECGE+++QL+  ++   KGL
Sbjct: 121 NCLADESSCVWNNGKIVQNMSLRLKNVECGEVVLQLEWIDLPGCKGL 165

[Close]

BLAST of Lsi04G011260 vs. NCBI nr
Match: gi|743888997|ref|XP_011038617.1| (PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD13 [Populus euphratica])

HSP 1 Score: 237.3 bits (604), Expect = 2.0e-59
Identity = 117/167 (70.06%), Postives = 135/167 (80.84%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M+N  GLLRIR+ RG+NLA+RD  T  SDPYVVI+   QK K+RVVKKNCNPEWNEE TL
Sbjct: 1   MDNLLGLLRIRVKRGNNLAVRDLGT--SDPYVVITMGKQKLKTRVVKKNCNPEWNEEITL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           SITD+N PI L VFDKDRFT DD MG+AEIDIK YI  L MGL+NLPNGCVV RV+PSR+
Sbjct: 61  SITDLNVPINLTVFDKDRFTVDDKMGEAEIDIKAYIASLKMGLQNLPNGCVVSRVKPSRN 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LADES  VW++GKI+QDM LRLRNVE GE+MIQ++  NV   +GL
Sbjct: 121 NCLADESCVVWDNGKILQDMILRLRNVESGEVMIQIEWINVPGCRGL 165

[Close]

BLAST of Lsi04G011260 vs. NCBI nr
Match: gi|224108311|ref|XP_002314800.1| (C2 domain-containing family protein [Populus trichocarpa])

HSP 1 Score: 235.3 bits (599), Expect = 7.8e-59
Identity = 115/167 (68.86%), Postives = 134/167 (80.24%), Query Frame = 1


Query: 1   MNNRAGLLRIRLLRGHNLAIRDAPTRSSDPYVVISCANQKFKSRVVKKNCNPEWNEEFTL 60
           M+N  GLLRIR+ RG+NLA+RD  T  SDPY VI+   QK K+RVVKKNCNPEWNEE TL
Sbjct: 1   MDNLLGLLRIRVKRGNNLAVRDLGT--SDPYAVITMGKQKLKTRVVKKNCNPEWNEELTL 60

Query: 61  SITDINTPIKLAVFDKDRFTKDDGMGDAEIDIKPYIECLNMGLENLPNGCVVKRVQPSRS 120
           SITD+N PI L VFDKDRFT DD MG+AEIDIK YI  L MGL+NLPNGCVV R++PSR+
Sbjct: 61  SITDLNVPINLTVFDKDRFTVDDKMGEAEIDIKAYIASLKMGLQNLPNGCVVSRIKPSRN 120

Query: 121 NSLADESPCVWNDGKIIQDMTLRLRNVECGEIMIQLQLFNVSIFKGL 168
           N LADES  VW++GKI+QDM LRLRNVE GE+MIQ++  NV   +GL
Sbjct: 121 NCLADESCVVWDNGKILQDMILRLRNVESGEVMIQIEWMNVPGCRGL 165

The following BLAST results are available for this feature:

BLAST of Lsi04G011260 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CAR10_ARATH	2.4e-52	61.31	Protein C2-DOMAIN ABA-RELATED 10 OS=Arabidopsis thaliana GN=CAR10 PE=2 SV=1	[more]
CAR9_ARATH	7.8e-51	58.54	Protein C2-DOMAIN ABA-RELATED 9 OS=Arabidopsis thaliana GN=CAR9 PE=2 SV=1	[more]
CAR4_ARATH	3.3e-49	56.89	Protein C2-DOMAIN ABA-RELATED 4 OS=Arabidopsis thaliana GN=CAR4 PE=1 SV=1	[more]
CAR5_ARATH	1.2e-48	58.60	Protein C2-DOMAIN ABA-RELATED 5 OS=Arabidopsis thaliana GN=CAR5 PE=2 SV=1	[more]
CAR7_ARATH	6.8e-47	56.29	Protein C2-DOMAIN ABA-RELATED 7 OS=Arabidopsis thaliana GN=CAR7 PE=1 SV=1	[more]

BLAST of Lsi04G011260 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KJA0_CUCSA	1.6e-90	93.60	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G030450 PE=4 SV=1	[more]
B9HW51_POPTR	5.4e-59	68.86	C2 domain-containing family protein OS=Populus trichocarpa GN=POPTR_0010s12100g ...	[more]
A5BXZ0_VITVI	3.5e-58	66.47	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0011g05230 PE=4 SV=...	[more]
A0A0A0KRJ0_CUCSA	5.1e-57	64.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G569360 PE=4 SV=1	[more]
W9QZY4_9ROSA	9.6e-56	66.87	ADP-ribosylation factor GTPase-activating protein AGD12 OS=Morus notabilis GN=L4...	[more]

BLAST of Lsi04G011260 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G01540.1	1.4e-53	61.31	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT1G70790.2	4.4e-52	58.54	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT3G17980.1	1.8e-50	56.89	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT1G48590.2	7.0e-50	58.60	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]
AT1G70810.1	3.8e-48	56.29	Calcium-dependent lipid-binding (CaLB domain) family protein	[more]

BLAST of Lsi04G011260 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449449040\|ref\|XP_004142273.1\|	2.2e-90	93.60	PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cuc...	[more]
gi\|659129460\|ref\|XP_008464698.1\|	6.5e-90	94.15	PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD11 [Cuc...	[more]
gi\|1009154994\|ref\|XP_015895476.1\|	7.5e-62	69.46	PREDICTED: protein C2-DOMAIN ABA-RELATED 7-like [Ziziphus jujuba]	[more]
gi\|743888997\|ref\|XP_011038617.1\|	2.0e-59	70.06	PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD13 [Pop...	[more]
gi\|224108311\|ref\|XP_002314800.1\|	7.8e-59	68.86	C2 domain-containing family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **INTERPRO**
Term	Definition
IPR000008	C2_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0046486	glycerolipid metabolic process
biological_process	GO:0006544	glycine metabolic process
biological_process	GO:0006563	L-serine metabolic process
biological_process	GO:0006566	threonine metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004609	phosphatidylserine decarboxylase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G011260.1	Lsi04G011260.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 domain	PRINTS	PR00360	C2DOMAIN	coord: 48..61 score: 5.1E-5coord: 24..36 score: 5.1E-5coord: 69..77 score: 5.
IPR000008	C2 domain	GENE3D	G3DSA:2.60.40.150		coord: 4..105 score: 3.7E-28coord: 137..158 score: 3.7
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 6..94 score: 8.9
IPR000008	C2 domain	SMART	SM00239	C2_3c	coord: 7..104 score: 1.1
IPR000008	C2 domain	PROFILE	PS50004	C2	coord: 1..89 score: 14
IPR000008	C2 domain	unknown	SSF49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 129..159 score: 2.11E-28coord: 2..93 score: 2.11
None	No IPR available	PANTHER	PTHR23180	CENTAURIN/ARF	coord: 1..155 score: 1.5
None	No IPR available	PANTHER	PTHR23180:SF268	CALCIUM-DEPENDENT LIPID-BINDING DOMAIN-CONTAINING PROTEIN	coord: 1..155 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G011260	Csa5G030450	Cucumber (Chinese Long) v2	culsiB372
Lsi04G011260	CSPI05G00800	Wild cucumber (PI 183967)	cpilsiB376
Lsi04G011260	Cucsa.009140	Cucumber (Gy14) v1	cgylsiB257
Lsi04G011260	CmaCh04G000620	Cucurbita maxima (Rimu)	cmalsiB680
Lsi04G011260	CmoCh04G000550	Cucurbita moschata (Rifu)	cmolsiB673
Lsi04G011260	Cla011718	Watermelon (97103) v1	lsiwmB303
Lsi04G011260	MELO3C026419	Melon (DHL92) v3.5.1	lsimeB286
Lsi04G011260	ClCG07G006030	Watermelon (Charleston Gray)	lsiwcgB296
Lsi04G011260	MELO3C026419.2	Melon (DHL92) v3.6.1	lsimedB301
Lsi04G011260	Cla97C07G134120	Watermelon (97103) v2	lsiwmbB297
Lsi04G011260	Bhi07G000300	Wax gourd	lsiwgoB389
Lsi04G011260	Cp4.1LG01g06090	Cucurbita pepo (Zucchini)	cpelsiB362
Lsi04G011260	CsGy5G011070	Cucumber (Gy14) v2	cgyblsiB340
Lsi04G011260	Carg22007	Silver-seed gourd	carlsiB159
Lsi04G011260	CsaV3_5G014650	Cucumber (Chinese Long) v3	cuclsiB396

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G011260	Cucurbita maxima (Rimu)	cmalsiB679
Lsi04G011260	Cucurbita moschata (Rifu)	cmolsiB672
Lsi04G011260	Cucurbita pepo (Zucchini)	cpelsiB361
Lsi04G011260	Silver-seed gourd	carlsiB610