Lsi03G010210 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi03G010210 vs. Swiss-Prot
Match: PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.4e-92
Identity = 183/383 (47.78%), Postives = 237/383 (61.88%), Query Frame = 1

Query: 3   IVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFE 62
           I ++S+  +V  +GA+G+G TDDSQAFLKAW+ VC    G     VP G TFML PLKF+
Sbjct: 17  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGT-GDGQFVVPAGMTFMLQPLKFQ 76

Query: 63  GPCNSKQVHFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDC 122
           G C S  V  ++ G ++APSKG W  D K+ WI F DI GL + G G+ +G+GS WW+  
Sbjct: 77  GSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEINGQGSSWWEHK 136

Query: 123 HSNCQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGI 182
            S    PTAL F +CN L+L    H++S  +H  I  CN   IS ++I AP+ SPNT GI
Sbjct: 137 GSR---PTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGI 196

Query: 183 DISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVEN 242
           D+  S+N++IQ+  I TGDDCI +N+G+SNI I G+ CGPGHGISIGSLG DGE + VEN
Sbjct: 197 DVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVEN 256

Query: 243 IYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDH----STFAYS 302
           + V NC  +GT NG RIKT QGG GYAR ITF  ITL + +N IIIDQ +    S  A  
Sbjct: 257 VCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKD 316

Query: 303 RKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMT--STPGKEVQ 362
           RK+ A  ++V  V +    GTS S   +  +C +   C+ I + D+ I T  S  G+  Q
Sbjct: 317 RKSSA--VEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQ 376

Query: 363 AFCQNVNGREESTVPSVSCLSKS 379
             C NV G     VP + CL  S
Sbjct: 377 GQCLNVRGASTIAVPGLECLELS 392

BLAST of Lsi03G010210 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.4e-73
Identity = 156/372 (41.94%), Postives = 222/372 (59.68%), Query Frame = 1

Query: 7   SSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCN 66
           +S  +V ++GA G+GKTDD+QAF KAWK  C    G+ T  VP GKT++L   +F GPC 
Sbjct: 65  ASTVSVSNFGAKGDGKTDDTQAFKKAWKKACST-NGVTTFLVPKGKTYLLKSTRFRGPCK 124

Query: 67  SKQVHFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSG--TGQFDGRGSFWWK---- 126
           S + +F++ GT+ A +K +   D K +W+   D+N L++ G  TG  +G G  WW+    
Sbjct: 125 SLR-NFQILGTLSASTKRSDYKD-KNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCK 184

Query: 127 -DCHSNC-QTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPN 186
            D    C + PTAL+ +N   L +K  +  N+ +   SIE CN   +S+++I AP DSPN
Sbjct: 185 IDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPN 244

Query: 187 TYGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYS 246
           T GI I+ + NI + NS I TGDDCI++ +G+ N++I  +TCGPGHGISIGSLG D   +
Sbjct: 245 TDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKA 304

Query: 247 IVENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYS 306
            V  I V       + NG+RIKT QGG G A+NI F+ I + + +N IIIDQD+      
Sbjct: 305 YVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDK 364

Query: 307 RKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQA-RCSNIVMEDVSIMTSTPGKEVQAF 366
            + Q   ++VK+V Y+ I GTS +  AI L C +   C  IV+E+V I   T      A 
Sbjct: 365 CEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIKGGT------AS 424

Query: 367 CQNVNGREESTV 370
           C+N N + + TV
Sbjct: 425 CKNANVKNQGTV 426

BLAST of Lsi03G010210 vs. Swiss-Prot
Match: ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 3.5e-72
Identity = 157/370 (42.43%), Postives = 220/370 (59.46%), Query Frame = 1

Query: 11  NVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQV 70
           +V D+GA G+GKTDD+QAF+ AWK  C +  G   L VP G T++L  ++  GPCNS  +
Sbjct: 69  SVSDFGAKGDGKTDDTQAFVNAWKKACSS-NGAVNLLVPKGNTYLLKSIQLTGPCNSI-L 128

Query: 71  HFELRGTIIAPSKGAWPSDSKE--NWIQFLDINGLTLSG--TGQFDGRGSFWWKD-CHSN 130
             ++ GT+ A  K    SD K+   WI F  +N L++ G  TG  DG G  WW++ C  N
Sbjct: 129 TVQIFGTLSASQKR---SDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRN 188

Query: 131 -----CQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTY 190
                 + PTAL+F+N   L +K  K  N+ +   SIE C++  +S++ + AP DSPNT 
Sbjct: 189 KAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTD 248

Query: 191 GIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIV 250
           GI I+ + NI +  S I TGDDCI++ +GS N++I  +TCGPGHGISIGSLG D   + V
Sbjct: 249 GIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFV 308

Query: 251 ENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRK 310
             + V    L GT NG+RIKT QGG G A NI F+ I + + +N IIIDQD+   +    
Sbjct: 309 SGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTT 368

Query: 311 AQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMTSTPGKEVQAFCQ 370
            ++  ++VK+V YR+I GTS S NAI   C +   C  IV++ V+I      K  +A C 
Sbjct: 369 EKSA-VQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI------KGGKATCT 426

BLAST of Lsi03G010210 vs. Swiss-Prot
Match: QRT2_ARATH (Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 6.0e-72
Identity = 149/368 (40.49%), Postives = 215/368 (58.42%), Query Frame = 1

Query: 5   SASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGP 64
           S+   FNV  +GA  NG  DDS+AF+KAW+  C +  GI  +  P  + +ML  + F GP
Sbjct: 66  SSPRSFNVNTFGAKANGN-DDSKAFMKAWEAACSST-GIVYIVAPKNRDYMLKAVTFSGP 125

Query: 65  CNSKQVHFELRGTIIAPSKGAW--PSDSKE--NWIQFLDINGLTLSGTGQFDGRGSFWW- 124
           C S  + F++ G I      AW  PSD KE  +WI F ++N L + G G+ DG G  WW 
Sbjct: 126 CKSSLIIFKIYGRI-----EAWENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWP 185

Query: 125 KDCHSNCQ-----TPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPK 184
           K C  N Q      PTA++F  CN L++   +  N+ + H + + C +    ++ + +P 
Sbjct: 186 KSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPA 245

Query: 185 DSPNTYGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGD 244
           DSPNT GI +S + NILIQ+S + TGDDCI++ +GS N++  G+TCGPGHGISIGSLG D
Sbjct: 246 DSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGED 305

Query: 245 GEYSIVENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHST 304
              + V N+ V+   L GT NG+RIKT QGG+G A+NI F+ I + +  N III+QD+  
Sbjct: 306 NSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCD 365

Query: 305 FAYSRKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMTSTPGKE 362
              +   Q   ++V +V Y+ I GTS+   A+   C +   C  I M++V ++  T    
Sbjct: 366 RVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDV 425

BLAST of Lsi03G010210 vs. Swiss-Prot
Match: PGLR_PERAE (Polygalacturonase OS=Persea americana PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.0e-67
Identity = 146/368 (39.67%), Postives = 206/368 (55.98%), Query Frame = 1

Query: 5   SASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGP 64
           S  +D +V D+GA G+G TDD++AF KAWKD C +      L VP  K ++L  + F GP
Sbjct: 80  SPDTDISVDDFGARGDG-TDDTKAFEKAWKDACSSGS---VLIVPENKNYLLKQITFSGP 139

Query: 65  CNSKQVHFELRGTIIAPS-KGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCH 124
           C S  +  ++RGTI A S +  W   +++ WI+F DI+ LTL G G  +G G  WW    
Sbjct: 140 CKS-DLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWD--- 199

Query: 125 SNCQ---------TPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPK 184
           S+C+          PTAL+F +C  L + +    +S K H S + C D I S++ + AP+
Sbjct: 200 SSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPE 259

Query: 185 DSPNTYGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGD 244
            SPNT GI I+ +  I + NS I TGDDCI++ +GS  +    +TCGPGHGISIGSLG  
Sbjct: 260 HSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDR 319

Query: 245 GEYSIVENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHST 304
              + V  + V    L  T NGLRIKT QGG G A+NI F+ I + +  N IIIDQ +  
Sbjct: 320 NSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCD 379

Query: 305 FAYSRKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQAR-CSNIVMEDVSIMTSTPGKE 362
                  Q   +KV +V Y  I GTS S  A+   C ++  C   ++ +++++    GKE
Sbjct: 380 SKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLV-GNGGKE 438

BLAST of Lsi03G010210 vs. TrEMBL
Match: A0A0A0KZ95_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 9.5e-149
Identity = 251/380 (66.05%), Postives = 305/380 (80.26%), Query Frame = 1

Query: 1   SWIVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLK 60
           SW V  SSDFNVLDYGAIGNGKTDDS+AFLKAW DVCGA E  PTLHVP GKT++LNPLK
Sbjct: 18  SWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEESPTLHVPDGKTYLLNPLK 77

Query: 61  FEGPCNSKQVHFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWK 120
           F+GPC SKQ    L GT++AP+K  W SD K  WIQF DI GL L G G+FDG+GS WWK
Sbjct: 78  FQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQGGGRFDGQGSLWWK 137

Query: 121 DC-HSNCQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNT 180
           DC H +C+ PTAL FHNCNGLQ+K  KHINS K+H SI  C+D I S + I AP+DSPNT
Sbjct: 138 DCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFSHLHISAPEDSPNT 197

Query: 181 YGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSI 240
            GIDIS+S NI I++S + TGDDCI +NNGSSNI I G+TCGPGHGISIGSLG DGEY++
Sbjct: 198 DGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGISIGSLGKDGEYNV 257

Query: 241 VENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSR 300
           VEN++VSNCLL+ TQNG+RIKT +GGYGYA+NITFEKIT+ + +N IIIDQ +S++AYSR
Sbjct: 258 VENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPIIIDQYYSSYAYSR 317

Query: 301 KAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQARCSNIVMEDVSIMTSTPGKEVQAFCQ 360
           K + M+IKV DVTYRE++GTS + +AI   C QARCSNI++++V+I  + PG++ +A CQ
Sbjct: 318 KMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIKMTNPGEKAKAVCQ 377

Query: 361 NVNGREESTVPSVSCLSKSN 380
           N +G+  S VP VSCLSKS+
Sbjct: 378 NADGKVLSAVPPVSCLSKSH 393

BLAST of Lsi03G010210 vs. TrEMBL
Match: A0A067JZL5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.2e-119
Identity = 214/380 (56.32%), Postives = 272/380 (71.58%), Query Frame = 1

Query: 2   WIVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKF 61
           WIV+ASS FNVLDYGAIG+GKTD+SQA L+AW+D+CGA  G PTL VP G TF+L P++F
Sbjct: 14  WIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAGGTFLLKPVRF 73

Query: 62  EGPCNSKQVHFELRGTIIAPSK-GAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWK 121
           +GPC SK +H +++GTI+A +   AW  D K +W+QF D+NGL + G GQ DG+G  WWK
Sbjct: 74  QGPCKSKNLHIQIQGTIVASNNINAWGKD-KGSWLQFSDVNGLIIDGGGQIDGQGGLWWK 133

Query: 122 DCHSN--CQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPN 181
            C  +  C  PTALSFHNCNGLQL   KH+NS ++H SI  C+D  +  + I AP+DSPN
Sbjct: 134 FCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHLYITAPQDSPN 193

Query: 182 TYGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYS 241
           T GIDIS S +I I+NS I TGDDCI +N  SS I I G++CGPGHGIS+GSLG +G Y 
Sbjct: 194 TDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISVGSLGKNGAYE 253

Query: 242 IVENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDH-STFAY 301
            VE ++V NC  KGTQNG+RIKT QGG GYAR ITF+ I L+ +QN IIIDQ++   F Y
Sbjct: 254 TVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIIIDQNYFCRFPY 313

Query: 302 SRKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQAR-CSNIVMEDVSIMTSTPGKEVQA 361
           S  A++ N+ + DVTYR I G+S+   AI L C + R C+NIVM+D+ I+ S+PG  + A
Sbjct: 314 S--AESSNVYISDVTYRNIRGSSSVERAIDLNCGRKRGCTNIVMDDIEIVPSSPGMHLHA 373

Query: 362 FCQNVNGREESTVPSVSCLS 377
           FC N  G      P+V CLS
Sbjct: 374 FCNNAYGISTLASPTVPCLS 390

BLAST of Lsi03G010210 vs. TrEMBL
Match: I1M7C8_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=2)

HSP 1 Score: 402.1 bits (1032), Expect = 7.3e-109
Identity = 196/371 (52.83%), Postives = 247/371 (66.58%), Query Frame = 1

Query: 10  FNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQ 69
           FNV+DYGAIG+G TDDSQAFLKAW  VC    G  TL VP GKTFML PL+F GPC+   
Sbjct: 32  FNVMDYGAIGDGLTDDSQAFLKAWSMVCAMKNGAATLKVPPGKTFMLKPLQFSGPCSFSS 91

Query: 70  VHFELRGTIIAP-SKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCH-SNCQ 129
           VHF+L G ++AP S  AW       WI F +++GL + G GQ DG GS WW  C   +C 
Sbjct: 92  VHFQLEGDVVAPKSTEAWKGQDSSKWIDFSNVDGLIIDGGGQIDGSGSVWWNSCKVKSCS 151

Query: 130 TPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGIDISKS 189
            PTALS  NCN LQL  T+H+NS ++H SI   N   I ++ I AP+DSPNT GID+S+S
Sbjct: 152 RPTALSIQNCNNLQLTGTRHLNSARNHISINNSNHTHIFNVTITAPQDSPNTDGIDVSQS 211

Query: 190 NNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENIYVSN 249
           + ILIQ S I TGDDCI + +G+S + I G+TCGPGHGIS+GSLG  G    VE+++V+N
Sbjct: 212 SYILIQRSTIATGDDCIAMKSGTSYVNITGITCGPGHGISVGSLGKKGTCQTVEHVHVNN 271

Query: 250 CLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQAMNIK 309
           C  KG  NG+RIKT  GG GYARNI FE I L +T+N IIIDQD+       K Q   ++
Sbjct: 272 CNFKGADNGMRIKTWPGGCGYARNIKFEHILLTNTKNPIIIDQDYENVQNEDKKQTSEVQ 331

Query: 310 VKDVTYREIHGTSTSHNAIVLKC-CQARCSNIVMEDVSIMTSTPGKEVQAFCQNVNGREE 369
           +  VTYR ++GTS S  AI+L C   A C++I M+ V+I +++ G  V A C N +G   
Sbjct: 332 ISGVTYRCVNGTSNSETAIILNCGAGAGCTDIFMDVVNITSTSSGSNVHASCNNAHGVAA 391

Query: 370 STVPSVSCLSK 378
           ST P VSCLS+
Sbjct: 392 STSPPVSCLSQ 402

BLAST of Lsi03G010210 vs. TrEMBL
Match: I1LNR1_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=2)

HSP 1 Score: 397.9 bits (1021), Expect = 1.4e-107
Identity = 196/370 (52.97%), Postives = 245/370 (66.22%), Query Frame = 1

Query: 10  FNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQ 69
           FNV+DYGAIG+G TDDSQAFLKAW  VC    G  TL VP GKTFML PL+F GPC+   
Sbjct: 32  FNVMDYGAIGDGLTDDSQAFLKAWSMVCAMKNGAATLKVPPGKTFMLKPLQFSGPCSFSS 91

Query: 70  VHFELRGTIIAP-SKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCH-SNCQ 129
           VHF+L G ++AP S  AW       WI F +++GL + G GQ DG GS WW  C   +C 
Sbjct: 92  VHFQLEGDVVAPKSTEAWKGQDSSKWIDFSNVDGLIIDGGGQIDGSGSVWWNSCKVKSCS 151

Query: 130 TPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGIDISKS 189
            PTALS HNCN LQL  T+H+NS ++H SI   N   I ++ I AP+DSPNT GID+S+S
Sbjct: 152 RPTALSIHNCNNLQLTGTRHLNSARNHISINNSNHTHIFNVTITAPQDSPNTDGIDVSQS 211

Query: 190 NNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENIYVSN 249
           + ILIQ S I TGDDCI +N+G+S + I G+TCGPGHGIS+GSLG  G    VE+++VSN
Sbjct: 212 SYILIQRSTIATGDDCIAMNSGTSYVNITGITCGPGHGISVGSLGKKGTCQTVEHVHVSN 271

Query: 250 CLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQAMNIK 309
           C  KG  NG+RIKT  GG GYARNI FE I L +T+N IIIDQD+             ++
Sbjct: 272 CNFKGADNGMRIKTWPGGCGYARNIKFEHIVLTNTKNPIIIDQDYE--------NTSEVQ 331

Query: 310 VKDVTYREIHGTSTSHNAIVLKC-CQARCSNIVMEDVSIMTSTPGKEVQAFCQNVNGREE 369
           +  VTYR ++GT  S  AI+L C   A C++I M+ V+I +++ G  V A C N +G   
Sbjct: 332 ISGVTYRYVNGTCNSETAIILNCGAGAGCTDIFMDLVNITSTSSGSNVLASCNNAHGVAA 391

Query: 370 STVPSVSCLS 377
           ST P VSCLS
Sbjct: 392 STSPPVSCLS 393

BLAST of Lsi03G010210 vs. TrEMBL
Match: A0A072U7D4_MEDTR (Polygalacturonase OS=Medicago truncatula GN=MTR_6g028080 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 6.9e-107
Identity = 200/376 (53.19%), Postives = 264/376 (70.21%), Query Frame = 1

Query: 10  FNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQ 69
           FNVLDYGA+GNGK D+++AF+ AWKD C AM    TL +P GKTFML P+ F+GPCNS  
Sbjct: 30  FNVLDYGAVGNGKNDETRAFVDAWKDTCIAMHESSTLLIPKGKTFMLQPVWFQGPCNSSN 89

Query: 70  VHFELRGTIIAPSK---GAWPSDS-KENWIQFLDINGLTLSGTGQFDGRGSFWWKDCHSN 129
           ++ +L GTIIAP       WP+D+ K +WI+F++I+GL + G G  DG+G+ WW DCHSN
Sbjct: 90  IYVKLEGTIIAPKNIEAWKWPNDNYKGSWIRFIEISGLIVYGGGIIDGKGAQWW-DCHSN 149

Query: 130 C----QTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYG 189
                + PTAL FH C  L L+E  HINS ++H S+ AC+   IS + IIAP +SPNT G
Sbjct: 150 IKCEKENPTALHFHACENLILRELTHINSPRNHISLNACHGSHISKLHIIAPNESPNTDG 209

Query: 190 IDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVE 249
           IDI++S+NI+I+NS IETGDDCI +N+GS++I I G+ CGPGHGIS+GSLG +G +  VE
Sbjct: 210 IDIAESSNIIIENSKIETGDDCIAINHGSNSIYINGIFCGPGHGISVGSLGRNGVHESVE 269

Query: 250 NIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKA 309
           NIYV NC    T NG RIKT  GG GYAR +TFE I L++  N +IIDQ+++ +      
Sbjct: 270 NIYVRNCTFNRTTNGARIKTWIGGNGYARKVTFEDIILIEADNPVIIDQEYNPY-----D 329

Query: 310 QAMNIKVKDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMTSTPGKEVQAFCQN 369
            A  +KV DVT+R I GTS + +AI L C +   C+NI++E ++I TS+ G+EV A C+N
Sbjct: 330 SAYAVKVSDVTFRNIRGTSIAKHAIQLHCDEDITCTNIILEGINI-TSSIGEEVHASCKN 389

Query: 370 VNGREESTVPSVSCLS 377
           VNG   S +P V CLS
Sbjct: 390 VNGVCTSCIPYVPCLS 398

BLAST of Lsi03G010210 vs. TAIR10
Match: AT3G15720.1 (AT3G15720.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 340.5 bits (872), Expect = 1.3e-93
Identity = 183/383 (47.78%), Postives = 237/383 (61.88%), Query Frame = 1

Query: 3   IVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFE 62
           I ++S+  +V  +GA+G+G TDDSQAFLKAW+ VC    G     VP G TFML PLKF+
Sbjct: 17  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGT-GDGQFVVPAGMTFMLQPLKFQ 76

Query: 63  GPCNSKQVHFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDC 122
           G C S  V  ++ G ++APSKG W  D K+ WI F DI GL + G G+ +G+GS WW+  
Sbjct: 77  GSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEINGQGSSWWEHK 136

Query: 123 HSNCQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGI 182
            S    PTAL F +CN L+L    H++S  +H  I  CN   IS ++I AP+ SPNT GI
Sbjct: 137 GSR---PTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGI 196

Query: 183 DISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVEN 242
           D+  S+N++IQ+  I TGDDCI +N+G+SNI I G+ CGPGHGISIGSLG DGE + VEN
Sbjct: 197 DVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVEN 256

Query: 243 IYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDH----STFAYS 302
           + V NC  +GT NG RIKT QGG GYAR ITF  ITL + +N IIIDQ +    S  A  
Sbjct: 257 VCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKD 316

Query: 303 RKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMT--STPGKEVQ 362
           RK+ A  ++V  V +    GTS S   +  +C +   C+ I + D+ I T  S  G+  Q
Sbjct: 317 RKSSA--VEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQ 376

Query: 363 AFCQNVNGREESTVPSVSCLSKS 379
             C NV G     VP + CL  S
Sbjct: 377 GQCLNVRGASTIAVPGLECLELS 392

BLAST of Lsi03G010210 vs. TAIR10
Match: AT4G35670.1 (AT4G35670.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 326.2 bits (835), Expect = 2.6e-89
Identity = 167/362 (46.13%), Postives = 229/362 (63.26%), Query Frame = 1

Query: 9   DFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSK 68
           ++NVL++ A G+G+TDDS+AFL+AW   CG    I TL +P GKTF+L P  F+GPC S 
Sbjct: 23  NYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQGPCKSS 82

Query: 69  QVHFELRGTIIAPS-KGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCHSNCQ 128
            +  +L GTI+APS K AW       WI+F  ++GL + G+G  D RGS +W+      Q
Sbjct: 83  SIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELNLKASQ 142

Query: 129 TPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGIDISKS 188
            PTAL    C+ L++     I+S K+H SI+ CN   IS+I + AP+ SPNT GIDIS S
Sbjct: 143 RPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGIDISDS 202

Query: 189 NNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENIYVSN 248
            NI I +S I+TGDDCI +N+GSSNI I G+ CGPGHGIS+GSLG  G  + V ++ V++
Sbjct: 203 TNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSDVQVTH 262

Query: 249 CLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQAMNIK 308
           C    T NG RIKT  GG GYARNI+F  ITL++T+N IIIDQ +      R  +   + 
Sbjct: 263 CTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHY--IDKGRLTEESAVA 322

Query: 309 VKDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMTSTPGKEVQAFCQNVNGREE 368
           + +V + +  GTS++ NAI LKC +   C ++VM+ + I  +  GK  +  CQ V+G   
Sbjct: 323 ISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMANGGKP-KVNCQYVDGESS 381

BLAST of Lsi03G010210 vs. TAIR10
Match: AT5G27530.1 (AT5G27530.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 326.2 bits (835), Expect = 2.6e-89
Identity = 165/372 (44.35%), Postives = 230/372 (61.83%), Query Frame = 1

Query: 10  FNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQ 69
           +NVL +GA G+G+TDDS AFL+AW   CG  E I TL +P GKT++L P++F+GPC S  
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 70  VHFELRGTIIAPSK-GAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCHSNCQT 129
           +  +L G I+APS   +W +   + WI F  + GL + G+G  +GRGS +W+        
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWE-------- 172

Query: 130 PTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGIDISKSN 189
             AL   NCN L +     I+S KSH SI+ C+   IS I I+AP++SPNT GIDIS S 
Sbjct: 173 --ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYST 232

Query: 190 NILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENIYVSNC 249
           N+ I +S I+TGDDCI +N GSS+I I  V CGPGHGIS+GSLG DGE + V ++YV+ C
Sbjct: 233 NVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQC 292

Query: 250 LLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQAMNIKV 309
               T NG RIKT QGG GYARNI+FE ITL++ QN IIIDQ ++        +   + +
Sbjct: 293 TFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAI 352

Query: 310 KDVTYREIHGTSTSHNAIVLKCCQ-ARCSNIVMEDVSIMTSTPGKEVQAFCQNVNGREES 369
             V Y    GT+ + +AI+LKC     C ++V++D+ + T   G++ +  C+NV G    
Sbjct: 353 SSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEV-TMENGEKPKVECENVEGESSD 412

Query: 370 TVPSVSCLSKSN 380
                 C + ++
Sbjct: 413 NDLMRECFNSNS 413

BLAST of Lsi03G010210 vs. TAIR10
Match: AT5G17200.1 (AT5G17200.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 313.5 bits (802), Expect = 1.7e-85
Identity = 162/375 (43.20%), Postives = 226/375 (60.27%), Query Frame = 1

Query: 11  NVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQV 70
           +V  +GAIG+GKTDD++AFLKAW+ VC        L VP GKTFML PL F GPC S  +
Sbjct: 40  SVTSFGAIGDGKTDDTKAFLKAWEAVCKGGHNRKIL-VPQGKTFMLKPLTFIGPCKSSTI 99

Query: 71  HFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCHSNCQTPT 130
              +RG ++AP    W +     WI F  INGL ++G G  DGRGS WW + ++    P 
Sbjct: 100 SLSIRGNLVAPGY-TWYAGRYTTWISFDSINGLVVTGGGTIDGRGSLWWGNVNNR---PC 159

Query: 131 ALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGIDISKSNNI 190
           A+ F+NCNGL++   +H+NS ++H  +    +  +  +++ AP DSPNT GIDIS    +
Sbjct: 160 AMHFNNCNGLRISNLRHLNSPRNHVGLSCSQNIEVRGLRMTAPGDSPNTDGIDISNCIGV 219

Query: 191 LIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENIYVSNCLL 250
            I +S I TGDDCI +N+GSS+I I G+ CGPGHGIS+GSLG  G++  VE + V NC  
Sbjct: 220 HIHDSVIATGDDCIAINSGSSHINITGIFCGPGHGISVGSLGVTGDFETVEEVRVKNCTF 279

Query: 251 KGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDH-----------STFAYSR 310
             TQNG+RIKT Q G GYAR I+FE I ++ ++N IIIDQ +           S+ +Y  
Sbjct: 280 TKTQNGVRIKTYQNGSGYARKISFEDINMVASENPIIIDQTYHNGGTNGGISKSSSSYQN 339

Query: 311 ----------KAQAMNIKVKDVTYREIHGTSTSHNAIVLKC-CQARCSNIVMEDVSIMTS 364
                           +KV DV Y  I G+S S   I L C     CS+IVM++V+++++
Sbjct: 340 CHLTAKQRTPSGNGKGVKVTDVRYARIRGSSASDQDITLNCDADLGCSDIVMDNVNMVSA 399

BLAST of Lsi03G010210 vs. TAIR10
Match: AT5G44840.1 (AT5G44840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 298.5 bits (763), Expect = 5.8e-81
Identity = 154/367 (41.96%), Postives = 219/367 (59.67%), Query Frame = 1

Query: 10  FNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQ 69
           ++VL++GA GNG TDDS+AF+KAW  +CG+     T  +P  KTF+L PL F+GPC S  
Sbjct: 24  YDVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNKTFLLQPLTFQGPCKSPS 83

Query: 70  VHFELRGTIIAP-SKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCHSNCQT 129
           V  +  G I+AP +K AW       W+ F +I GLT++G+G   GRGS +WK  H     
Sbjct: 84  VQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTIHGRGSSFWKQLH----- 143

Query: 130 PTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGIDISKSN 189
                F  CN L +      NS K+H SI  C    I+ I+++AP DSPNT GI+IS+S+
Sbjct: 144 -----FQRCNDLMITGITSFNSPKNHISISECKRVKITKIKLVAPHDSPNTDGINISESS 203

Query: 190 NILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENIYVSNC 249
           ++ I ++ I TGDDC+ +N+GS NI I  + CGPGHGIS+GS+G DGE SIVEN+ V+NC
Sbjct: 204 DVDIYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSVGRDGEESIVENVQVTNC 263

Query: 250 LLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQAMNIKV 309
               T NG RIKT   G GYA+NI F+ +T  +T+N IIIDQ++         +   + +
Sbjct: 264 TFIRTDNGARIKTWPNGKGYAKNILFKSLTFRETKNPIIIDQNYVDKG-RLDVEESAVAI 323

Query: 310 KDVTYREIHGTSTSHNAIVLKCCQAR-CSNIVMEDVSIMTSTPGKEVQAFCQNVNGREES 369
            +VT+ ++ GTS     I + C +   C +IV++ + I T    K +   C NV G+  +
Sbjct: 324 SNVTFTDLRGTSKLDEIIKIDCSKVTYCKDIVLDKIDIATVDGNKPI-VECSNVYGKSIN 378

Query: 370 TVPSVSC 375
              +  C
Sbjct: 384 ANEANGC 378

BLAST of Lsi03G010210 vs. NCBI nr
Match: gi|778697492|ref|XP_011654334.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 1.4e-148
Identity = 251/380 (66.05%), Postives = 305/380 (80.26%), Query Frame = 1

Query: 1   SWIVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLK 60
           SW V  SSDFNVLDYGAIGNGKTDDS+AFLKAW DVCGA E  PTLHVP GKT++LNPLK
Sbjct: 18  SWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEESPTLHVPDGKTYLLNPLK 77

Query: 61  FEGPCNSKQVHFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWK 120
           F+GPC SKQ    L GT++AP+K  W SD K  WIQF DI GL L G G+FDG+GS WWK
Sbjct: 78  FQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQGGGRFDGQGSLWWK 137

Query: 121 DC-HSNCQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNT 180
           DC H +C+ PTAL FHNCNGLQ+K  KHINS K+H SI  C+D I S + I AP+DSPNT
Sbjct: 138 DCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFSHLHISAPEDSPNT 197

Query: 181 YGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSI 240
            GIDIS+S NI I++S + TGDDCI +NNGSSNI I G+TCGPGHGISIGSLG DGEY++
Sbjct: 198 DGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGISIGSLGKDGEYNV 257

Query: 241 VENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSR 300
           VEN++VSNCLL+ TQNG+RIKT +GGYGYA+NITFEKIT+ + +N IIIDQ +S++AYSR
Sbjct: 258 VENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPIIIDQYYSSYAYSR 317

Query: 301 KAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQARCSNIVMEDVSIMTSTPGKEVQAFCQ 360
           K + M+IKV DVTYRE++GTS + +AI   C QARCSNI++++V+I  + PG++ +A CQ
Sbjct: 318 KMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDNVNIKMTNPGEKAKAVCQ 377

Query: 361 NVNGREESTVPSVSCLSKSN 380
           N +G+  S VP VSCLSKS+
Sbjct: 378 NADGKVLSAVPPVSCLSKSH 393

BLAST of Lsi03G010210 vs. NCBI nr
Match: gi|659108957|ref|XP_008454473.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo])

HSP 1 Score: 515.4 bits (1326), Expect = 8.5e-143
Identity = 240/376 (63.83%), Postives = 299/376 (79.52%), Query Frame = 1

Query: 4   VSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEG 63
           V +SSDFNVLDYGAIGNGKTDDS+AFLKAW DVCGA +  PTLHVP GKT++L+PLKF+G
Sbjct: 21  VLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDSPTLHVPDGKTYLLSPLKFQG 80

Query: 64  PCNSKQVHFELRGTIIAPSKGAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWKDCH 123
           PC S QV F+L GT++AP+K  WP+D K  W+QF+D+ GL + G G+FDG+GS WWK   
Sbjct: 81  PCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGLNVQGGGRFDGQGSCWWK--- 140

Query: 124 SNCQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGID 183
                  AL FHNCNGLQ+   KH+NS K+H SI  CND I S + I AP+DSPNT GID
Sbjct: 141 -------ALFFHNCNGLQMNGMKHVNSAKNHISINLCNDVIFSHLHISAPEDSPNTDGID 200

Query: 184 ISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENI 243
           IS+S NI IQ+S I TGDDC+ +NNGSSNI I  +TCGPGHGISIGSLG DGEY++VEN+
Sbjct: 201 ISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPGHGISIGSLGKDGEYNVVENV 260

Query: 244 YVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQA 303
           +VSNCLL+ TQNG+RIKT +GGYGYA+NITFEKITL +++N IIIDQ ++++AYSRK + 
Sbjct: 261 HVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSKNPIIIDQYYTSYAYSRKIKG 320

Query: 304 MNIKVKDVTYREIHGTSTSHNAIVLKCCQARCSNIVMEDVSIMTSTPGKEVQAFCQNVNG 363
           M+IKV DVTYRE++GTS +  AI   C QARCSNI++++V+I  + PG+E +A CQNV+G
Sbjct: 321 MDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDNVNIKMTNPGEEAKAVCQNVDG 380

Query: 364 REESTVPSVSCLSKSN 380
           +  S VP VSCLSKS+
Sbjct: 381 KVLSAVPPVSCLSKSH 386

BLAST of Lsi03G010210 vs. NCBI nr
Match: gi|802681849|ref|XP_012082168.1| (PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas])

HSP 1 Score: 438.0 bits (1125), Expect = 1.7e-119
Identity = 214/380 (56.32%), Postives = 272/380 (71.58%), Query Frame = 1

Query: 2   WIVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKF 61
           WIV+ASS FNVLDYGAIG+GKTD+SQA L+AW+D+CGA  G PTL VP G TF+L P++F
Sbjct: 14  WIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAGGTFLLKPVRF 73

Query: 62  EGPCNSKQVHFELRGTIIAPSK-GAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWK 121
           +GPC SK +H +++GTI+A +   AW  D K +W+QF D+NGL + G GQ DG+G  WWK
Sbjct: 74  QGPCKSKNLHIQIQGTIVASNNINAWGKD-KGSWLQFSDVNGLIIDGGGQIDGQGGLWWK 133

Query: 122 DCHSN--CQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPN 181
            C  +  C  PTALSFHNCNGLQL   KH+NS ++H SI  C+D  +  + I AP+DSPN
Sbjct: 134 FCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHLYITAPQDSPN 193

Query: 182 TYGIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYS 241
           T GIDIS S +I I+NS I TGDDCI +N  SS I I G++CGPGHGIS+GSLG +G Y 
Sbjct: 194 TDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISVGSLGKNGAYE 253

Query: 242 IVENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDH-STFAY 301
            VE ++V NC  KGTQNG+RIKT QGG GYAR ITF+ I L+ +QN IIIDQ++   F Y
Sbjct: 254 TVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIIIDQNYFCRFPY 313

Query: 302 SRKAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQAR-CSNIVMEDVSIMTSTPGKEVQA 361
           S  A++ N+ + DVTYR I G+S+   AI L C + R C+NIVM+D+ I+ S+PG  + A
Sbjct: 314 S--AESSNVYISDVTYRNIRGSSSVERAIDLNCGRKRGCTNIVMDDIEIVPSSPGMHLHA 373

Query: 362 FCQNVNGREESTVPSVSCLS 377
           FC N  G      P+V CLS
Sbjct: 374 FCNNAYGISTLASPTVPCLS 390

BLAST of Lsi03G010210 vs. NCBI nr
Match: gi|1000944382|ref|XP_002530440.2| (PREDICTED: probable polygalacturonase At3g15720 [Ricinus communis])

HSP 1 Score: 415.6 bits (1067), Expect = 9.2e-113
Identity = 204/379 (53.83%), Postives = 269/379 (70.98%), Query Frame = 1

Query: 2   WIVSASSDFNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKF 61
           W  +ASS FNVLDY A+G+GKTDDS+ FLKAW+DVC A +G+PTL +P G+TF+L P+ F
Sbjct: 58  WEAAASSTFNVLDYNAVGDGKTDDSEVFLKAWRDVCEATKGMPTLILPSGRTFLLKPIIF 117

Query: 62  EGPCNSKQVHFELRGTIIAPSK-GAWPSDSKENWIQFLDINGLTLSGTGQFDGRGSFWWK 121
           +GPC S  +HF+++G+I+AP+   AW +D K NW+QF D+NGL + G GQ DG+GS WWK
Sbjct: 118 QGPCKSTYLHFQIQGSILAPNDVTAWEND-KTNWLQFSDVNGLMIDGGGQIDGQGSVWWK 177

Query: 122 DCHSNCQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTY 181
                         +NCN LQL + +H+NS ++H SI  CN   IS++ +IAP++SPNT 
Sbjct: 178 QS----------LVYNCNALQLSKLRHVNSQRNHISINGCNGVDISELSMIAPEESPNTD 237

Query: 182 GIDISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIV 241
           GIDIS S++I I+NS I TGDDCI +N  +SNI I GV CGPGHGIS+GSLG +G Y  V
Sbjct: 238 GIDISASSHINIRNSLISTGDDCIAINGFTSNINISGVLCGPGHGISVGSLGKNGAYETV 297

Query: 242 ENIYVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDH-STFAYSR 301
           E+I+V NC   GTQNG+RIKT  GG GYAR I+FE+I L+ +QN IIIDQ++   F Y R
Sbjct: 298 EDIHVENCTFNGTQNGVRIKTWPGGSGYARRISFEQINLIASQNPIIIDQNYWDHFQYGR 357

Query: 302 KAQAMNIKVKDVTYREIHGTSTSHNAIVLKCCQAR-CSNIVMEDVSIMTSTPGKEVQAFC 361
             ++ ++ + DVTYREI GTS    AI L C ++  CSNIVM+++ I+ STPG ++ AFC
Sbjct: 358 --ESSDVHISDVTYREIRGTSADLRAISLNCGKSTGCSNIVMDNIDIIPSTPGMQLYAFC 417

Query: 362 QNVNGREESTVPSVSCLSK 378
            N  G   S  P+V CLSK
Sbjct: 418 NNAYGSSTSASPNVPCLSK 423

BLAST of Lsi03G010210 vs. NCBI nr
Match: gi|502092038|ref|XP_004489698.1| (PREDICTED: probable polygalacturonase At3g15720 [Cicer arietinum])

HSP 1 Score: 406.8 bits (1044), Expect = 4.3e-110
Identity = 201/375 (53.60%), Postives = 267/375 (71.20%), Query Frame = 1

Query: 10  FNVLDYGAIGNGKTDDSQAFLKAWKDVCGAMEGIPTLHVPIGKTFMLNPLKFEGPCNSKQ 69
           FNVLDYGA+GNGK D++QAF+ AWKD C A+   PTL +P GKTF+L PL F+GPCNS  
Sbjct: 30  FNVLDYGAVGNGKNDETQAFVDAWKDTCSAIHDSPTLLIPKGKTFILQPLWFQGPCNSTT 89

Query: 70  VHFELRGTIIAPSK---GAWPSDS-KENWIQFLDINGLTLSGTGQFDGRGSFWWKDCHSN 129
           V+ +L GTI AP+K     WP+++ KE WI+F +INGL + G+GQ DG+G+ WW DC+SN
Sbjct: 90  VYVKLEGTIKAPNKIEAWKWPNNNEKETWIRFSEINGLIIYGSGQIDGQGAPWW-DCYSN 149

Query: 130 --CQTPTALSFHNCNGLQLKETKHINSGKSHFSIEACNDGIISDIQIIAPKDSPNTYGID 189
             C  PTAL FH C  L L+   HINS ++H S+ AC+   IS + IIAP +SPNT GID
Sbjct: 150 TNCDRPTALHFHACENLILRGLTHINSPRNHISLNACHGSHISKLHIIAPNESPNTDGID 209

Query: 190 ISKSNNILIQNSAIETGDDCITLNNGSSNIKIVGVTCGPGHGISIGSLGGDGEYSIVENI 249
           I++S+N++I+NS +ETGDDCI +N+GSS+I I+G+ CGPGHGIS+GSLG +G    VENI
Sbjct: 210 IAESSNVIIENSKMETGDDCIAINHGSSSINIIGIFCGPGHGISVGSLGRNGADESVENI 269

Query: 250 YVSNCLLKGTQNGLRIKTLQGGYGYARNITFEKITLMDTQNSIIIDQDHSTFAYSRKAQA 309
           YV NC   GT NG RIKT  GG+GYAR +TFE I +++  N +IIDQ+++ +        
Sbjct: 270 YVRNCTFNGTTNGARIKTWMGGHGYARKVTFEDIIVIEAYNPVIIDQEYNPY-----DSV 329

Query: 310 MNIKVKDVTYREIHGTSTSHNAIVLKCCQA-RCSNIVMEDVSIMTSTPGKEVQAFCQNVN 369
             +KV DVT+R I GTS S +AI L C +A  C+NI++E ++I TS+ G+EV A C+N  
Sbjct: 330 HAVKVSDVTFRNIRGTSISKHAIQLNCDEAISCTNIILEGINI-TSSIGEEVHASCKNAK 389

Query: 370 GREESTVPSVSCLSK 378
           G   S +P V CLS+
Sbjct: 390 GMCTSCIPYVPCLSQ 397