Cp4.1LG14g04560 (gene) Cucurbita pepo (Zucchini)

Overview
NameCp4.1LG14g04560
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionPectin lyase-like superfamily protein
LocationCp4.1LG14 : 1544350 .. 1547336 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTAGCCTCGGCGGCCTGCACGAACGCGATCGAGCTCGTGAGCGACGGTGGAAATGAAATGGAAAGTGAGTTTGGAAGCAAGATGATGAAAAATGAGCTTCCAATCCCAATCCCTAGGTGTGATCTTGTACTAAGACGATGCGATTCTGACTGCGACTGCCCTTCATACTGCGTTTGCATGCAAGGAACCTGCACCTGAAAATATCCATCCATTTCATATGCCTCCGACCCCGACCCCGATCCCGACTCCTACTACTATTACTACCGACTCCTACTACTATTACTATTACTTTTACTACTATTACGCTTTGTGTGTTTTGGCTTTCCTTTTCTTAAGTTCTCTATAATTACAATAAATCTCTCGTCGGTCGGTCTCTGTTTAAGTGATCGGTTTATGTAGTAAAAATCTTACGATCTATAAATCAATAAAGTTGTTTTGTTACAGTATGTTTTGTTCTCACTCACATGCATTCCAAAATGATTGGCTGTGTGTTATCATTTGTAACGGTCTATGCCCATCACTAGCACATATTGTCTGTTTTGGTTTGTTACGTATCGTTGTCAGTCTCACAGTTTTAAAACGTGTTTTTTAGAGAGAGGTTTCCACACCCTTGTTAGAAATGTTTCATTCCCCTCTTCAACCAAAGTGGGATCTCACAATCTACCAGCTAGGGGCCAACGTGCTCATTAGCACAATGCTCAATATATATACTATTTGTAACCATCTAAGAAGAAAATTTGACTCTGATACAATTTGTAACAGTGAAAAAAAATTATCTGTCTTGCATTGTTTGACTCGAGAAATGAAAATTACTATTTTTTTCGGATCCAAAATCTAGATTTTACAGGCTAGTGTTACCTCATTTCCTCGGGTATAAATAACCATATTTATTATTTGTCTAGAACAATTTTTAAGAACGAAGAACATATAGAACAACATGTTACGAACAATCATTTTTATCTACTTGAAAATCGATCACCAATAATTTGAAAGAGATTATAACGGGTGGAAAAATTAGTTGTTACGAACGATATTTTTTTCATATAAATAATTAACGAGAGGATTATTAATCTTGATTAAGTTACCAAAAAAGATAATCAAATCCAAATTTGGAGCAAATTGATTGATATTTAATACACCAAAACTATTAATTTCTAAAAAAATTAAGTGTTTCCATCAAAAGATTTATGAACATACTAAAATTAGAAGACCCATTAATTTGTCACCTAAAATAAAATGTCCCCAACATTTGCTTTCTTTAAGCCTATAATTTCCATACTTCCAAATTAATTTCCCATTTTACCCCTCATGCTTTGTTTATAAACTCGACTCGATATCTCCATTCATACCAGACATTTTGGGGTCCTCAAATCTTCTCTCGTTCTTGCTTTCGTATCGTTAGATTAGAAATGATGAAGCTACTTTCTGCTTTAGTTTTTATGTTTTCCACTGTCATTTTAAGCTCGTGGATTGTATTAGCTTCATCTGATTTCAATGTTCTTGATTATGGAGCGGTTGGAAATGGAAAAACCGATGATTCACAAGTAATTGCTTTGACTCGAGTTTTGTTTATGTACGTATAGTTCCGTCTTTTCTTATTAGTCTTTCTTGATCTCTTTTTGTGGTGATAAGGCTTTTTTGAAAGCATGGAAAGACGTGTGTCGGGCATCGACAGGCATCCCAACATTGCGTGTACCAATTGGAAAAACTTACTTGTTGAAGCCTCTCAAATTCGAGGGTCCTTGTAACCCTAAACGAGTACATTTTGAGGTAACTATTGCTACTAAAATTGCCTTCGTAATTGTAAGATTCCACATCAGTTGGAGAGAGGAACGAAGCATTCCTTACAAGGGTGTAGAAACCTCTCTTTAGTAGATGTGTTTTAAAACCGCAAAGTTGACAACGATACATAACGAGTCAAAACAGACAATAGTTATTAGTAGTGAGCTTAGACTGATACACTGTTGTTGTTTCAAAAGGTTAGAGGAACAATAATGGCTCCGAGCGTGGCTGCATGGCGTAGCCATAACGAAGAAAACTGGATTCAATTCTTGGACATAGCGGGTCTCAATATCAATGGAGCAGGTCAATTCGATGGCCAGAGTTCTTCTTGGTGGAAAGATTGCCATTCAAACTGCACACCTCCAACGGTAAGTTTTTAACGATCTAATTCTAGGGTTTGATCGTTAAACTGATAACGTGAATATTAATTATATTATATGGTTTTACAGGCATTATTTTTCCAAAATTGCACCGGGCTCCAACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCTTAAACGCTTGCAATGATGTCGTTATCTCAAACGTTCACATCATTGCTCCCGAAAACATCCCTAACACATACGGATTCAACATTTCTAGATCAAATAATGTTCTTATTCAAAACTCCTTAATCGAAACAGGTATCAAATTTGTATTACTTCGTTTTCAAACTGACTTAGAATTTCATAATGGGTTTCTAATTTTATCGTAGGTGACGAATGTATTTCTCTATACAACGGTTCTTCTAATATCAATATCGTCGGTGTTACATGTGGACCTGGTTACGGTATAAGGTACGAAAACGACATTTTCCGTATTATAATTTCATTGTTTTTGTATTAATTTATAATTATCATCTTAATTTCTATAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATTGAAAACATCAATGTAAGCAATTGCACCTTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTACAAAGGTTAATTATAAGTAGTTTTTAATTTATTCATATGCAATTTTTTTTAATATATATATTACCAAAAAATGACGGGTTTTCTTAACGTAACTACAGGGTGGACATGGATATGTAAGAAACGTCATATACGAAAAAATAACGCTAATTGATACCCAATTGCCGATCGTTATTGATCAACAGTATTCAACTCTTGCCAACACGAGAAAAATT

mRNA sequence

GTTTAGCCTCGGCGGCCTGCACGAACGCGATCGAGCTCGTGAGCGACGGTGGAAATGAAATGGAAAGTGAGTTTGGAAGCAAGATGATGAAAAATGAGCTTCCAATCCCAATCCCTAGATTAGAAATGATGAAGCTACTTTCTGCTTTAGTTTTTATGTTTTCCACTGTCATTTTAAGCTCGTGGATTGTATTAGCTTCATCTGATTTCAATGTTCTTGATTATGGAGCGGTTGGAAATGGAAAAACCGATGATTCACAAGCTTTTTTGAAAGCATGGAAAGACGTGTGTCGGGCATCGACAGGCATCCCAACATTGCGTGTACCAATTGGAAAAACTTACTTGTTGAAGCCTCTCAAATTCGAGGCGGGTCTCAATATCAATGGAGCAGGTCAATTCGATGGCCAGAGTTCTTCTTGGTGGAAAGATTGCCATTCAAACTGCACACCTCCAACGGCATTATTTTTCCAAAATTGCACCGGGCTCCAACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCTTAAACGCTTGCAATGATGTCGTTATCTCAAACGTTCACATCATTGCTCCCGAAAACATCCCTAACACATACGGATTCAACATTTCTAGATCAAATAATGTTCTTATTCAAAACTCCTTAATCGAAACAGGTGACGAATGTATTTCTCTATACAACGGTTCTTCTAATATCAATATCGTCGGTGTTACATGTGGACCTGGTTACGGTATAAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATTGAAAACATCAATGTAAGCAATTGCACCTTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTACAAAGGGTGGACATGGATATGTAAGAAACGTCATATACGAAAAAATAACGCTAATTGATACCCAATTGCCGATCGTTATTGATCAACAGTATTCAACTCTTGCCAACACGAGAAAAATT

Coding sequence (CDS)

ATGGAAAGTGAGTTTGGAAGCAAGATGATGAAAAATGAGCTTCCAATCCCAATCCCTAGATTAGAAATGATGAAGCTACTTTCTGCTTTAGTTTTTATGTTTTCCACTGTCATTTTAAGCTCGTGGATTGTATTAGCTTCATCTGATTTCAATGTTCTTGATTATGGAGCGGTTGGAAATGGAAAAACCGATGATTCACAAGCTTTTTTGAAAGCATGGAAAGACGTGTGTCGGGCATCGACAGGCATCCCAACATTGCGTGTACCAATTGGAAAAACTTACTTGTTGAAGCCTCTCAAATTCGAGGCGGGTCTCAATATCAATGGAGCAGGTCAATTCGATGGCCAGAGTTCTTCTTGGTGGAAAGATTGCCATTCAAACTGCACACCTCCAACGGCATTATTTTTCCAAAATTGCACCGGGCTCCAACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCTTAAACGCTTGCAATGATGTCGTTATCTCAAACGTTCACATCATTGCTCCCGAAAACATCCCTAACACATACGGATTCAACATTTCTAGATCAAATAATGTTCTTATTCAAAACTCCTTAATCGAAACAGGTGACGAATGTATTTCTCTATACAACGGTTCTTCTAATATCAATATCGTCGGTGTTACATGTGGACCTGGTTACGGTATAAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATTGAAAACATCAATGTAAGCAATTGCACCTTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTACAAAGGGTGGACATGGATATGTAAGAAACGTCATATACGAAAAAATAACGCTAATTGATACCCAATTGCCGATCGTTATTGATCAACAGTATTCAACTCTTGCCAACACGAGAAAAATT

Protein sequence

MESEFGSKMMKNELPIPIPRLEMMKLLSALVFMFSTVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEAGLNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYSTLANTRKI
Homology
[Close]
BLAST of Cp4.1LG14g04560 vs. Swiss-Prot
Match: PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.0e-54
Identity = 120/287 (41.81%), Postives = 169/287 (58.89%), Query Frame = 1 

Query: 43  IVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFE 102
           I  +S+  +V  +GAVG+G TDDSQAFLKAW+ VC + TG     VP G T++L+PLKF+
Sbjct: 17  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVC-SGTGDGQFVVPAGMTFMLQPLKFQ 76

Query: 103 AGLN-----INGAGQFDGQSSSWWK-------------------DCHSNC---------- 162
                    +   G+    S   WK                   D   N           
Sbjct: 77  GSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVIEGDGEINGQGSSWWEHKG 136

Query: 163 TPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISR 222
           + PTAL F++C  L+L    H++S   HI ++ CN V IS++ I APE+ PNT G ++  
Sbjct: 137 SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGA 196

Query: 223 SNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVS 282
           S+NV+IQ+ +I TGD+CI++ +G+SNI+I G+ CGPG+GISI SLG+DG    +EN+ V 
Sbjct: 197 SSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQ 256

Query: 283 NCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYS 296
           NC  +GT NG RIKT +GG GY R + +  ITL + + PI+IDQ Y+
Sbjct: 257 NCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYN 302

[Close]
BLAST of Cp4.1LG14g04560 vs. Swiss-Prot
Match: QRT2_ARATH (Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 3.7e-48
Identity = 108/288 (37.50%), Postives = 158/288 (54.86%), Query Frame = 1 

Query: 50  FNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKF-------- 109
           FNV  +GA  NG  DDS+AF+KAW+  C +STGI  +  P  + Y+LK + F        
Sbjct: 71  FNVNTFGAKANGN-DDSKAFMKAWEAAC-SSTGIVYIVAPKNRDYMLKAVTFSGPCKSSL 130

Query: 110 ---------EA--------------------GLNINGAGQFDGQSSSWW-KDCHSN---- 169
                    EA                     L + G G+ DG    WW K C  N    
Sbjct: 131 IIFKIYGRIEAWENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWPKSCKINPQLP 190

Query: 170 CT-PPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNI 229
           C   PTA+ F  C  L++   +  N+ + H++   C +V   N+ + +P + PNT G ++
Sbjct: 191 CLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHV 250

Query: 230 SRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENIN 289
           S + N+LIQ+S++ TGD+CIS+ +GS N+   G+TCGPG+GISI SLGED     + N+ 
Sbjct: 251 SGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSEAYVSNVV 310

Query: 290 VSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
           V+  TL GT NG+RIKT +GGHG  +N+I++ I + +   PI+I+Q Y
Sbjct: 311 VNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDY 356

[Close]
BLAST of Cp4.1LG14g04560 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.2e-47
Identity = 112/303 (36.96%), Postives = 162/303 (53.47%), Query Frame = 1 

Query: 36  TVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYL 95
           ++I S    L +S  +V ++GA G+GKTDD+QAF KAWK  C ++ G+ T  VP GKTYL
Sbjct: 54  SLISSDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYL 113

Query: 96  LKP------------------------------------LKFEAGLNINGA--GQFDGQS 155
           LK                                     L+    L+I+G   G  +G  
Sbjct: 114 LKSTRFRGPCKSLRNFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNG 173

Query: 156 SSWWK-----DCHSNCTP-PTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVH 215
            +WW+     D    CT  PTAL   N   L ++  +  N+ +  IS+  CN V +SNV 
Sbjct: 174 KTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVE 233

Query: 216 IIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIR 275
           I AP + PNT G +I+ + N+ + NS I TGD+CIS+ +G+ N+ I  +TCGPG+GISI 
Sbjct: 234 ITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIG 293

Query: 276 SLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVID 295
           SLG+D     +  INV       + NG+RIKT +GG G  +N+ ++ I + + + PI+ID
Sbjct: 294 SLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPIIID 353

[Close]
BLAST of Cp4.1LG14g04560 vs. Swiss-Prot
Match: PGLR_PERAE (Polygalacturonase OS=Persea americana PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.8e-42
Identity = 103/294 (35.03%), Postives = 152/294 (51.70%), Query Frame = 1 

Query: 48  SDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFE----- 107
           +D +V D+GA G+G TDD++AF KAWKD C + +    L VP  K YLLK + F      
Sbjct: 83  TDISVDDFGARGDG-TDDTKAFEKAWKDACSSGS---VLIVPENKNYLLKQITFSGPCKS 142

Query: 108 ---------------------------------AGLNINGAGQFDGQSSSWWKDCHSNC- 167
                                            + L + G G  +G   +WW    S+C 
Sbjct: 143 DLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWD---SSCK 202

Query: 168 --------TPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPN 227
                   + PTAL F++C  L + +    +S K H+S + C DV+ SN+ + APE+ PN
Sbjct: 203 RKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPN 262

Query: 228 TYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYN 287
           T G +I+ +  + + NS+I TGD+CIS+ +GS  +    +TCGPG+GISI SLG+     
Sbjct: 263 TDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEA 322

Query: 288 VIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
            +  + V    L  T NGLRIKT +GG G  +N+ ++ I + +   PI+IDQ Y
Sbjct: 323 HVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYY 369

[Close]
BLAST of Cp4.1LG14g04560 vs. Swiss-Prot
Match: PGLR_ACTDE (Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.4e-41
Identity = 101/292 (34.59%), Postives = 152/292 (52.05%), Query Frame = 1 

Query: 46  ASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEA-- 105
           AS   NV D+GA G+G+ DD++AF KAWK  C +ST    L VP  K YL++P+ F    
Sbjct: 86  ASKTVNVDDFGAKGDGR-DDTKAFEKAWKAAC-SSTSSAVLLVP-KKNYLVRPISFSGPC 145

Query: 106 -----------------------------------GLNINGAGQFDGQSSSWWKD-CHSN 165
                                               L + G G  +G    WW++ C +N
Sbjct: 146 KSGLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTN 205

Query: 166 CT-----PPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTY 225
                   PTAL F     + ++  K  N+ + H+S + C +V  SN+ + APEN PNT 
Sbjct: 206 KALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPNTD 265

Query: 226 GFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVI 285
           G +++ + N+ I + +I TGD+CIS+ NGS  + +  +TCGPG+GISI SLG       +
Sbjct: 266 GIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEAHV 325

Query: 286 ENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
            ++ V+   L GT NG+RIKT +GG G   N+ ++ + + + + PI+IDQ Y
Sbjct: 326 SDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNY 374

[Close]
BLAST of Cp4.1LG14g04560 vs. TrEMBL
Match: A0A0A0KZ95_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.7e-100
Identity = 182/315 (57.78%), Postives = 228/315 (72.38%), Query Frame = 1 

Query: 24  MKLLSALVFMFSTVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGI 83
           MKLLSA +F++S  ILSSW VL SSDFNVLDYGA+GNGKTDDS+AFLKAW DVC A+   
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 84  PTLRVPIGKTYLLKPLKFEA----------------------------------GLNING 143
           PTL VP GKTYLL PLKF+                                   GLN+ G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 144 AGQFDGQSSSWWKDC-HSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVIS 203
            G+FDGQ S WWKDC H +C  PTALFF NC GLQ++  KHINS KNHIS+N C+DV+ S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 204 NVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGI 263
           ++HI APE+ PNT G +IS S N+ I++S + TGD+CI++ NGSSNINI G+TCGPG+GI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 264 SIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPI 304
           SI SLG+DG YNV+EN++VSNC L+ TQNG+RIKT +GG+GY +N+ +EKIT+ + + PI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

[Close]
BLAST of Cp4.1LG14g04560 vs. TrEMBL
Match: A0A067JZL5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.2e-76
Identity = 146/299 (48.83%), Postives = 196/299 (65.55%), Query Frame = 1 

Query: 36  TVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYL 95
           T++   WIV ASS FNVLDYGA+G+GKTD+SQA L+AW+D+C A+ G PTL VP G T+L
Sbjct: 8   TILSFFWIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAGGTFL 67

Query: 96  LKPLKFE--------------------------------------AGLNINGAGQFDGQS 155
           LKP++F+                                       GL I+G GQ DGQ 
Sbjct: 68  LKPVRFQGPCKSKNLHIQIQGTIVASNNINAWGKDKGSWLQFSDVNGLIIDGGGQIDGQG 127

Query: 156 SSWWKDCHSN--CTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAP 215
             WWK C  +  C+ PTAL F NC GLQL   KH+NS +NHIS+N C+DV + +++I AP
Sbjct: 128 GLWWKFCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHLYITAP 187

Query: 216 ENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGE 275
           ++ PNT G +IS S ++ I+NS+I TGD+CI++   SS INI G++CGPG+GIS+ SLG+
Sbjct: 188 QDSPNTDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISVGSLGK 247

Query: 276 DGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
           +G Y  +E ++V NCT KGTQNG+RIKT +GG GY R + ++ I LI +Q PI+IDQ Y
Sbjct: 248 NGAYETVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIIIDQNY 306

[Close]
BLAST of Cp4.1LG14g04560 vs. TrEMBL
Match: V4STK3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027137mg PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 2.5e-67
Identity = 136/284 (47.89%), Postives = 170/284 (59.86%), Query Frame = 1 

Query: 50  FNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKF-------- 109
           +NV+++GAVGNG TDDSQAF+KAW  VC  ++  PTL+VP GKT+LL+P  F        
Sbjct: 29  YNVMNFGAVGNGVTDDSQAFIKAWNAVCGDTSNNPTLQVPQGKTFLLQPTSFQGPCKSSN 88

Query: 110 -------------------------------EAGLNINGAGQFDGQSSSWWKDCHSNCTP 169
                                            G  +NG G+ DGQ S WWK C      
Sbjct: 89  LQVQIEGNLIAPEGPSSWKGKDRRSWLYFANVNGFTVNGNGEIDGQGSQWWKLCSD---- 148

Query: 170 PTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISRSN 229
             AL F+ CT  Q+   KHINS KNHISL+ C D  +SN+HI APE+ PNT G +IS S 
Sbjct: 149 --ALVFEKCTSFQVSGLKHINSQKNHISLDNCQDATLSNLHISAPESSPNTDGIDISASQ 208

Query: 230 NVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNC 289
           N+ I NS I TGD+CI++  GSS IN+ G+TCGPG+GISI SLG+ G    +E ++V NC
Sbjct: 209 NIHILNSNIATGDDCIAINTGSSQINVTGLTCGPGHGISIGSLGKQGEAAAVEEVHVKNC 268

Query: 290 TLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
           TL  TQNGLRIKT +GG GY R + +  ITL D   PI+IDQ Y
Sbjct: 269 TLNATQNGLRIKTWQGGSGYARKITFNDITLTDVDNPIIIDQFY 306

[Close]
BLAST of Cp4.1LG14g04560 vs. TrEMBL
Match: G7JAS3_MEDTR (Polygalacturonase/glycoside hydrolase family protein OS=Medicago truncatula GN=MTR_3g084360 PE=3 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 2.8e-66
Identity = 134/294 (45.58%), Postives = 179/294 (60.88%), Query Frame = 1 

Query: 45  LASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEA- 104
           LA   FNV++YGA G+  TDDS AFLKAW++VC ++ G PTL +P GK ++L+P  F+  
Sbjct: 19  LAQKSFNVVNYGAKGDSNTDDSNAFLKAWQEVCGSTQGTPTLIIPGGKKFMLQPSSFQGP 78

Query: 105 ----------------------------------------GLNINGAGQFDGQSSSWWKD 164
                                                   GL I+G G FDGQ +SWWK+
Sbjct: 79  CKSSTVKVMMMGTIIAPQSSGKWKWDSRDDDAWISFSHIKGLIISGEGTFDGQGASWWKN 138

Query: 165 CHSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYG 224
            +++   PTAL F  C  L+L   +HI+S KNHIS+  CN  +IS++HIIAPEN PNT G
Sbjct: 139 -YNDHGRPTALQFLGCDNLKLGPLRHIDSPKNHISIVDCNGALISHLHIIAPENSPNTDG 198

Query: 225 FNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIE 284
            +ISRS N++I++S I TGD+CI++ +GS  INI  + CGPG+GIS+ SLG+DG Y  +E
Sbjct: 199 IDISRSTNIIIEHSTISTGDDCIAINSGSKFINITAINCGPGHGISVGSLGKDGKYATVE 258

Query: 285 NINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYSTL 298
            ++VS+    GT+NG RIKT   G GY R + YE ITLI    PI+IDQ Y  L
Sbjct: 259 EVHVSHSIFTGTENGARIKTWTAGTGYARKITYEDITLIKVNNPIIIDQHYDAL 311

[Close]
BLAST of Cp4.1LG14g04560 vs. TrEMBL
Match: U5FZU8_POPTR (Glycoside hydrolase family 28 family protein OS=Populus trichocarpa GN=POPTR_0012s12010g PE=3 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.4e-64
Identity = 132/293 (45.05%), Postives = 175/293 (59.73%), Query Frame = 1 

Query: 50  FNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEAGL---- 109
           FNVLDYGA GNGK D + AF KAW D C+AS  +PTL VP GKT+LLK + F        
Sbjct: 18  FNVLDYGADGNGKIDATPAFQKAWGDFCQASEEMPTLEVPAGKTFLLKSVSFSGPCMSKN 77

Query: 110 -------NINGAGQFD----------------------------GQSSSWWKDCHSN--- 169
                   I     FD                            G+  +WWK C+ N   
Sbjct: 78  PHVLIEGTIVAPNSFDSWDDDDYEKWIGFTAVVGLIVDGGGRFDGRGEAWWKACNDNDSA 137

Query: 170 CTP-PTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNI 229
           C+    AL F  C GL+L    H+NS K HI +NAC+DV +SN+ I+AP+  PNT G +I
Sbjct: 138 CSKRRQALHFNKCNGLRLSNLHHVNSQKGHICINACDDVEVSNLQILAPDESPNTDGIDI 197

Query: 230 SRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENIN 289
           S SN+V I +S I TGD+CI++   S++IN+ GV CGPG+GISI SLG+DG Y  +E+++
Sbjct: 198 SESNHVNIHDSFIGTGDDCIAINGFSTSINVTGVKCGPGHGISIGSLGKDGAYETVEDVH 257

Query: 290 VSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYSTLAN 300
           V +C  KGTQNG+RIKT + G GYVR + +E IT ++++ PI+IDQQY+   N
Sbjct: 258 VKSCAFKGTQNGVRIKTWETGSGYVRKITFEDITFVNSENPIIIDQQYNPNGN 310

[Close]
BLAST of Cp4.1LG14g04560 vs. TAIR10
Match: AT5G17200.1 (AT5G17200.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 218.4 bits (555), Expect = 6.1e-57
Identity = 110/281 (39.15%), Postives = 162/281 (57.65%), Query Frame = 1 

Query: 51  NVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEA------- 110
           +V  +GA+G+GKTDD++AFLKAW+ VC+       L VP GKT++LKPL F         
Sbjct: 40  SVTSFGAIGDGKTDDTKAFLKAWEAVCKGGHNRKIL-VPQGKTFMLKPLTFIGPCKSSTI 99

Query: 111 ------------------------------GLNINGAGQFDGQSSSWWKDCHSNCTPPTA 170
                                         GL + G G  DG+ S WW + ++    P A
Sbjct: 100 SLSIRGNLVAPGYTWYAGRYTTWISFDSINGLVVTGGGTIDGRGSLWWGNVNNR---PCA 159

Query: 171 LFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISRSNNVL 230
           + F NC GL++   +H+NS +NH+ L+   ++ +  + + AP + PNT G +IS    V 
Sbjct: 160 MHFNNCNGLRISNLRHLNSPRNHVGLSCSQNIEVRGLRMTAPGDSPNTDGIDISNCIGVH 219

Query: 231 IQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLK 290
           I +S+I TGD+CI++ +GSS+INI G+ CGPG+GIS+ SLG  G +  +E + V NCT  
Sbjct: 220 IHDSVIATGDDCIAINSGSSHINITGIFCGPGHGISVGSLGVTGDFETVEEVRVKNCTFT 279

Query: 291 GTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
            TQNG+RIKT + G GY R + +E I ++ ++ PI+IDQ Y
Sbjct: 280 KTQNGVRIKTYQNGSGYARKISFEDINMVASENPIIIDQTY 316

[Close]
BLAST of Cp4.1LG14g04560 vs. TAIR10
Match: AT5G27530.1 (AT5G27530.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 216.1 bits (549), Expect = 3.0e-56
Identity = 121/280 (43.21%), Postives = 161/280 (57.50%), Query Frame = 1 

Query: 50  FNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEA------ 109
           +NVL +GA G+G+TDDS AFL+AW   C     I TL +P GKTYLL+P++F+       
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 110 -GLNINGAGQFDGQSSSWWKDCHSNCTPPT---------------------------ALF 169
             L ++G        +SW     SN    T                           AL 
Sbjct: 113 IKLQLDGIIVAPSNITSW-----SNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWEALH 172

Query: 170 FQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQ 229
             NC  L +     I+S K+HIS+  C+ V IS ++I+APEN PNT G +IS S NV I 
Sbjct: 173 ICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYSTNVNIF 232

Query: 230 NSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 289
           +S I+TGD+CI++  GSS+INI  V CGPG+GIS+ SLG DG    + ++ V+ CT   T
Sbjct: 233 DSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQCTFNKT 292

Query: 290 QNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYS 296
            NG RIKT +GG GY RN+ +E ITLI+ Q PI+IDQQY+
Sbjct: 293 TNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYT 327

[Close]
BLAST of Cp4.1LG14g04560 vs. TAIR10
Match: AT3G15720.1 (AT3G15720.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 214.2 bits (544), Expect = 1.1e-55
Identity = 120/287 (41.81%), Postives = 169/287 (58.89%), Query Frame = 1 

Query: 43  IVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFE 102
           I  +S+  +V  +GAVG+G TDDSQAFLKAW+ VC + TG     VP G T++L+PLKF+
Sbjct: 17  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVC-SGTGDGQFVVPAGMTFMLQPLKFQ 76

Query: 103 AGLN-----INGAGQFDGQSSSWWK-------------------DCHSNC---------- 162
                    +   G+    S   WK                   D   N           
Sbjct: 77  GSCKSTPVFVQMLGKLVAPSKGNWKGDKDQWILFTDIEGLVIEGDGEINGQGSSWWEHKG 136

Query: 163 TPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISR 222
           + PTAL F++C  L+L    H++S   HI ++ CN V IS++ I APE+ PNT G ++  
Sbjct: 137 SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGA 196

Query: 223 SNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVS 282
           S+NV+IQ+ +I TGD+CI++ +G+SNI+I G+ CGPG+GISI SLG+DG    +EN+ V 
Sbjct: 197 SSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVCVQ 256

Query: 283 NCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYS 296
           NC  +GT NG RIKT +GG GY R + +  ITL + + PI+IDQ Y+
Sbjct: 257 NCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYN 302

[Close]
BLAST of Cp4.1LG14g04560 vs. TAIR10
Match: AT5G44840.1 (AT5G44840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 213.4 bits (542), Expect = 2.0e-55
Identity = 114/301 (37.87%), Postives = 169/301 (56.15%), Query Frame = 1 

Query: 33  MFSTVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGK 92
           +F   +L S I++    ++VL++GA GNG TDDS+AF+KAW  +C +     T  +P  K
Sbjct: 8   VFLLTLLHS-ILVNGQIYDVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNK 67

Query: 93  TYLLKPL---------------------------------------KFEAGLNINGAGQF 152
           T+LL+PL                                       K   GL +NG+G  
Sbjct: 68  TFLLQPLTFQGPCKSPSVQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTI 127

Query: 153 DGQSSSWWKDCHSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHII 212
            G+ SS+WK  H          FQ C  L +      NS KNHIS++ C  V I+ + ++
Sbjct: 128 HGRGSSFWKQLH----------FQRCNDLMITGITSFNSPKNHISISECKRVKITKIKLV 187

Query: 213 APENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSL 272
           AP + PNT G NIS S++V I +++I TGD+C+++ +GS NINI  + CGPG+GIS+ S+
Sbjct: 188 APHDSPNTDGINISESSDVDIYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSV 247

Query: 273 GEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQ 295
           G DG  +++EN+ V+NCT   T NG RIKT   G GY +N++++ +T  +T+ PI+IDQ 
Sbjct: 248 GRDGEESIVENVQVTNCTFIRTDNGARIKTWPNGKGYAKNILFKSLTFRETKNPIIIDQN 297

[Close]
BLAST of Cp4.1LG14g04560 vs. TAIR10
Match: AT4G35670.1 (AT4G35670.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 204.9 bits (520), Expect = 7.0e-53
Identity = 113/294 (38.44%), Postives = 165/294 (56.12%), Query Frame = 1 

Query: 44  VLASSDFNVLDYGAVGNGKTDDSQAFLKAW----------------------------KD 103
           ++   ++NVL++ A G+G+TDDS+AFL+AW                            + 
Sbjct: 18  IVYGKNYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQG 77

Query: 104 VCRASTGIPTLRVPIGKTYLLKPLKFE---------------AGLNINGAGQFDGQSSSW 163
            C++S+    ++V +  T +    KF                +GL I G+G  D + SS+
Sbjct: 78  PCKSSS----IKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSF 137

Query: 164 WKDCHSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPN 223
           W+        PTAL    C  L++     I+S KNHIS+  CN V ISN+++ APE  PN
Sbjct: 138 WELNLKASQRPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPN 197

Query: 224 TYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYN 283
           T G +IS S N+ I +S I+TGD+CI++ +GSSNINI G+ CGPG+GIS+ SLG  G   
Sbjct: 198 TDGIDISDSTNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEA 257

Query: 284 VIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
            + ++ V++CT   T NG RIKT  GG GY RN+ +  ITL++T+ PI+IDQ Y
Sbjct: 258 KVSDVQVTHCTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHY 307

[Close]
BLAST of Cp4.1LG14g04560 vs. NCBI nr
Match: gi|778697492|ref|XP_011654334.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus])

HSP 1 Score: 374.0 bits (959), Expect = 2.5e-100
Identity = 182/315 (57.78%), Postives = 228/315 (72.38%), Query Frame = 1 

Query: 24  MKLLSALVFMFSTVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGI 83
           MKLLSA +F++S  ILSSW VL SSDFNVLDYGA+GNGKTDDS+AFLKAW DVC A+   
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 84  PTLRVPIGKTYLLKPLKFEA----------------------------------GLNING 143
           PTL VP GKTYLL PLKF+                                   GLN+ G
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLGGTLMAPTKDEWISDGKGQWIQFFDIEGLNLQG 120

Query: 144 AGQFDGQSSSWWKDC-HSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVIS 203
            G+FDGQ S WWKDC H +C  PTALFF NC GLQ++  KHINS KNHIS+N C+DV+ S
Sbjct: 121 GGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHDVIFS 180

Query: 204 NVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGI 263
           ++HI APE+ PNT G +IS S N+ I++S + TGD+CI++ NGSSNINI G+TCGPG+GI
Sbjct: 181 HLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGPGHGI 240

Query: 264 SIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPI 304
           SI SLG+DG YNV+EN++VSNC L+ TQNG+RIKT +GG+GY +N+ +EKIT+ + + PI
Sbjct: 241 SIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNAKNPI 300

[Close]
BLAST of Cp4.1LG14g04560 vs. NCBI nr
Match: gi|659108957|ref|XP_008454473.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo])

HSP 1 Score: 344.0 bits (881), Expect = 2.7e-91
Identity = 174/318 (54.72%), Postives = 218/318 (68.55%), Query Frame = 1 

Query: 24  MKLLSALVFMFSTVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGI 83
           MKLL A +  +S  ILSS  VL+SSDFNVLDYGA+GNGKTDDS+AFLKAW DVC A+   
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 84  PTLRVPIGKTYLLKPLKFEA--------------------------------------GL 143
           PTL VP GKTYLL PLKF+                                       GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 144 NINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDV 203
           N+ G G+FDGQ S WWK          ALFF NC GLQ+   KH+NS KNHIS+N CNDV
Sbjct: 121 NVQGGGRFDGQGSCWWK----------ALFFHNCNGLQMNGMKHVNSAKNHISINLCNDV 180

Query: 204 VISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPG 263
           + S++HI APE+ PNT G +IS+S N+ IQ+S I TGD+C+++ NGSSNINI  +TCGPG
Sbjct: 181 IFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPG 240

Query: 264 YGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQ 304
           +GISI SLG+DG YNV+EN++VSNC L+ TQNG+RIKT +GG+GY +N+ +EKITL +++
Sbjct: 241 HGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSK 300

[Close]
BLAST of Cp4.1LG14g04560 vs. NCBI nr
Match: gi|802681849|ref|XP_012082168.1| (PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas])

HSP 1 Score: 293.9 bits (751), Expect = 3.2e-76
Identity = 146/299 (48.83%), Postives = 196/299 (65.55%), Query Frame = 1 

Query: 36  TVILSSWIVLASSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYL 95
           T++   WIV ASS FNVLDYGA+G+GKTD+SQA L+AW+D+C A+ G PTL VP G T+L
Sbjct: 8   TILSFFWIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAGGTFL 67

Query: 96  LKPLKFE--------------------------------------AGLNINGAGQFDGQS 155
           LKP++F+                                       GL I+G GQ DGQ 
Sbjct: 68  LKPVRFQGPCKSKNLHIQIQGTIVASNNINAWGKDKGSWLQFSDVNGLIIDGGGQIDGQG 127

Query: 156 SSWWKDCHSN--CTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAP 215
             WWK C  +  C+ PTAL F NC GLQL   KH+NS +NHIS+N C+DV + +++I AP
Sbjct: 128 GLWWKFCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHLYITAP 187

Query: 216 ENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGE 275
           ++ PNT G +IS S ++ I+NS+I TGD+CI++   SS INI G++CGPG+GIS+ SLG+
Sbjct: 188 QDSPNTDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISVGSLGK 247

Query: 276 DGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
           +G Y  +E ++V NCT KGTQNG+RIKT +GG GY R + ++ I LI +Q PI+IDQ Y
Sbjct: 248 NGAYETVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIIIDQNY 306

[Close]
BLAST of Cp4.1LG14g04560 vs. NCBI nr
Match: gi|985430158|ref|XP_015385669.1| (PREDICTED: probable polygalacturonase At3g15720 [Citrus sinensis])

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-70
Identity = 139/286 (48.60%), Postives = 175/286 (61.19%), Query Frame = 1 

Query: 50  FNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKF-------- 109
           +NV+++GAVGNG TDDSQAF+KAW  VC  ++  PTL+VP GKT+LL+P  F        
Sbjct: 16  YNVMNFGAVGNGVTDDSQAFIKAWNAVCGDTSNNPTLQVPQGKTFLLQPTSFQGPCKSSN 75

Query: 110 -------------------------------EAGLNINGAGQFDGQSSSWWKDCHS--NC 169
                                            G  +NG G+ DGQ S WWK C    +C
Sbjct: 76  LQVQIEGNLIAPEGPSSWKGKDRRSWLYFVNVNGFTVNGNGEIDGQGSQWWKLCSDVGDC 135

Query: 170 TPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISR 229
           T PTAL F+ CT  Q+   KHINS KNHISL+ C D  +SN+HI APE+ PNT G +IS 
Sbjct: 136 TKPTALVFEKCTSFQVSGLKHINSQKNHISLDNCQDATLSNLHISAPESSPNTDGIDISA 195

Query: 230 SNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVS 289
           S N+ I NS I TGD+C+++  GSS IN+ G+TCGPG+GISI SLG+ G    +E ++V 
Sbjct: 196 SQNIHILNSNIATGDDCVAINTGSSQINVTGLTCGPGHGISIGSLGKQGEAAAVEEVHVK 255

Query: 290 NCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQY 295
           NCTL  TQNGLRIKT +GG GY R + +  ITL D   PI+IDQ Y
Sbjct: 256 NCTLNATQNGLRIKTWQGGSGYARKITFNDITLTDVDNPIIIDQFY 301

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BLAST of Cp4.1LG14g04560 vs. NCBI nr
Match: gi|1009123377|ref|XP_015878509.1| (PREDICTED: probable polygalacturonase At3g15720 [Ziziphus jujuba])

HSP 1 Score: 274.6 bits (701), Expect = 2.0e-70
Identity = 138/287 (48.08%), Postives = 177/287 (61.67%), Query Frame = 1 

Query: 53  LDYGAVGNGKTDDSQAFLKAWKDVCRASTGIPTLRVPIGKTYLLKPLKFEA--------- 112
           +DYGAVG+G+TDDS AFLKAW D+C AS G  TL +P  KT+LLKPL F           
Sbjct: 1   MDYGAVGDGQTDDSPAFLKAWGDLCGASEGTATLNIPKEKTFLLKPLTFSGPCNSSNINF 60

Query: 113 ----------------------------GLNINGAGQFDGQSSSWWKDCHSNCTPPTALF 172
                                       GL +NG G  DGQ S+WW  C +      AL 
Sbjct: 61  QIEGKLVAPNMGSWSGDKSMWISFDHVEGLVVNGGGSIDGQGSAWWNACGNE-----ALH 120

Query: 173 FQNCTGLQLRETKHINSVKNHISLNACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQ 232
           F  C GL L +  H++S KNHISL +CN+V I N+H+ AP+  PNT G +IS S ++ I 
Sbjct: 121 FNQCHGLHLSDLSHLDSPKNHISLKSCNNVHIVNLHVTAPKESPNTDGIDISESADIHID 180

Query: 233 NSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGT 292
           +S++ TGD+CI + NGSS INI G+TCGPG+GIS+ SLG +G YN +EN+ V NCT KGT
Sbjct: 181 DSVMATGDDCIGINNGSSYINIKGITCGPGHGISVGSLGGNGAYNTVENVYVFNCTFKGT 240

Query: 293 QNGLRIKTTKGGHGYVRNVIYEKITLIDTQLPIVIDQQYSTLANTRK 303
           QNG RIKT++GG GY RN+ +EKI + D + PI+IDQQY     + K
Sbjct: 241 QNGARIKTSQGGSGYARNITFEKIIVEDCENPIIIDQQYDASVESNK 282

The following BLAST results are available for this feature:
BLAST of Cp4.1LG14g04560 vs. Swiss-Prot
Match NameE-valueIdentityDescription
PGLR3_ARATH2.0e-5441.81Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 S... [more]
QRT2_ARATH3.7e-4837.50Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2[more]
ADPG1_ARATH9.2e-4736.96Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1[more]
PGLR_PERAE1.8e-4235.03Polygalacturonase OS=Persea americana PE=2 SV=1[more]
PGLR_ACTDE3.4e-4134.59Polygalacturonase OS=Actinidia deliciosa PE=2 SV=1[more]
BLAST of Cp4.1LG14g04560 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KZ95_CUCSA1.7e-10057.78Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1[more]
A0A067JZL5_JATCU2.2e-7648.83Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1[more]
V4STK3_9ROSI2.5e-6747.89Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027137mg PE=3 SV=1[more]
G7JAS3_MEDTR2.8e-6645.58Polygalacturonase/glycoside hydrolase family protein OS=Medicago truncatula GN=M... [more]
U5FZU8_POPTR4.4e-6445.05Glycoside hydrolase family 28 family protein OS=Populus trichocarpa GN=POPTR_001... [more]
BLAST of Cp4.1LG14g04560 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G17200.16.1e-5739.15 Pectin lyase-like superfamily protein[more]
AT5G27530.13.0e-5643.21 Pectin lyase-like superfamily protein[more]
AT3G15720.11.1e-5541.81 Pectin lyase-like superfamily protein[more]
AT5G44840.12.0e-5537.87 Pectin lyase-like superfamily protein[more]
AT4G35670.17.0e-5338.44 Pectin lyase-like superfamily protein[more]
BLAST of Cp4.1LG14g04560 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778697492|ref|XP_011654334.1|2.5e-10057.78PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus][more]
gi|659108957|ref|XP_008454473.1|2.7e-9154.72PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo][more]
gi|802681849|ref|XP_012082168.1|3.2e-7648.83PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas][more]
gi|985430158|ref|XP_015385669.1|1.6e-7048.60PREDICTED: probable polygalacturonase At3g15720 [Citrus sinensis][more]
gi|1009123377|ref|XP_015878509.1|2.0e-7048.08PREDICTED: probable polygalacturonase At3g15720 [Ziziphus jujuba][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: INTERPRO
TermDefinition
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR006626PbH1
IPR000743Glyco_hydro_28
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0071555 cell wall organization
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG14g04560.1Cp4.1LG14g04560.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 105..300
score: 6.2
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 271..292
score: 5300.0coord: 189..210
score: 380.0coord: 242..263
score: 39.0coord: 162..188
score: 390.0coord: 212..232
score: 60
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 36..296
score: 7.43
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 45..296
score: 1.3
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 22..294
score: 3.7E
NoneNo IPR availablePANTHERPTHR31375:SF42SUBFAMILY NOT NAMEDcoord: 22..294
score: 3.7E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cp4.1LG14g04560CmaCh16G003200Cucurbita maxima (Rimu)cmacpeB344
Cp4.1LG14g04560CmoCh16G003380Cucurbita moschata (Rifu)cmocpeB307
Cp4.1LG14g04560Cla006426Watermelon (97103) v1cpewmB204
Cp4.1LG14g04560ClCG10G010400Watermelon (Charleston Gray)cpewcgB190
Cp4.1LG14g04560Lsi03G010210Bottle gourd (USVL1VR-Ls)cpelsiB168
Cp4.1LG14g04560Carg19686Silver-seed gourdcarcpeB0407
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cp4.1LG14g04560Cucurbita pepo (Zucchini)cpecpeB234
Cp4.1LG14g04560Cucumber (Gy14) v1cgycpeB0188
Cp4.1LG14g04560Cucumber (Gy14) v1cgycpeB0215
Cp4.1LG14g04560Cucurbita maxima (Rimu)cmacpeB114
Cp4.1LG14g04560Cucurbita maxima (Rimu)cmacpeB715
Cp4.1LG14g04560Cucurbita moschata (Rifu)cmocpeB092
Cp4.1LG14g04560Cucurbita moschata (Rifu)cmocpeB668
Cp4.1LG14g04560Wild cucumber (PI 183967)cpecpiB221
Cp4.1LG14g04560Cucumber (Chinese Long) v2cpecuB229
Cp4.1LG14g04560Cucumber (Chinese Long) v2cpecuB219
Cp4.1LG14g04560Melon (DHL92) v3.5.1cpemeB200
Cp4.1LG14g04560Melon (DHL92) v3.5.1cpemeB201
Cp4.1LG14g04560Melon (DHL92) v3.5.1cpemeB182
Cp4.1LG14g04560Melon (DHL92) v3.5.1cpemeB208
Cp4.1LG14g04560Cucumber (Gy14) v2cgybcpeB485
Cp4.1LG14g04560Cucumber (Gy14) v2cgybcpeB492
Cp4.1LG14g04560Cucumber (Gy14) v2cgybcpeB747
Cp4.1LG14g04560Melon (DHL92) v3.6.1cpemedB220
Cp4.1LG14g04560Melon (DHL92) v3.6.1cpemedB243
Cp4.1LG14g04560Melon (DHL92) v3.6.1cpemedB242
Cp4.1LG14g04560Melon (DHL92) v3.6.1cpemedB250
Cp4.1LG14g04560Silver-seed gourdcarcpeB0523
Cp4.1LG14g04560Cucumber (Chinese Long) v3cpecucB0245
Cp4.1LG14g04560Cucumber (Chinese Long) v3cpecucB0263
Cp4.1LG14g04560Cucumber (Chinese Long) v3cpecucB0269
Cp4.1LG14g04560Cucumber (Chinese Long) v3cpecucB0283
Cp4.1LG14g04560Wax gourdcpewgoB0266
Cp4.1LG14g04560Wax gourdcpewgoB0304