Lsi03G004080 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi03G004080
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	WRKY transcription factor 60
Location	chr03 : 4656714 .. 4658408 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCAGCCTTATAAACTACTCCAATTAATTAAACCCTCAGATTGAGATTAATATTAATATGGATAATAATTACCAAATCTTCTTCCCAGATTCCTCCTCCTCTCATAATTCACAGCCTACTACGCCCATGGATCATGATCAGTTCGAAGAAGAAGAAGATCAAGAAGAAGAAGAAAGTAGTGTGAAGAAAACAAGTGGATCAGGGAAATCGAAGAATGAGAAGATAAGGAAAGGAAGATTTGCGTTTGAAACCAGGAGCCAAGTTGATGTGCTTGACGATGGCTATCGCTGGAGAAAATATGGTCAAAAGGCTGTTAAGAACAACAAGTTTCCCAGGTTTCATTATTTCTTATCCTTTTTCAGATCAATCACCTTCTTCTTAATTTTTCATTTCTTTCACTTCTGAGTTGACTGAATATGATTTATGTAAATTTTGAATTTACAGAGATTAAATCTGAATGTAAGAGATCTCGAAAATATGATTAAGAAAACCTTGAAAGAATTGATAGTTATTACTTATATCAACAAAGTACATTTTTCTTTTTAGTAGTTGAATCATAACTCATAAAAACTCCAAAATTAATAGAATCGTGTTTTTAGAGCACGTTGAAAAGACGTAGATTTTTTTGTCAGGTCATAGAAGATGCAAAGAAAATGTTGAGGTCATCAAGTGTCAAATTAGAATTTTGAATCTTAATCTTAGAATATTACAATTTAAGATAATACCATAACAAGATTTTTAAAATTGAAAAAAATTAAAAAGGTTGTAGTCAGAGAGAATAAATTTTGATAAATGTTCAACAAACACAAAATTTTGTAGTTATGCTCACAATATTGAAATATATATATATATATATATATATATATATATATATATATATATATATTGAGATAACCAAATTTAGTGTAACTTGTTATAGCTTTTGTGGTCGATAGTGATTTAACAGGTATCAAATCAAGATCATGTTTTGTGTCTAAAAAAGAAAAATCAAGTTTTGTGCTCAAACTCTAATGCACGTCATCTTTAATCAATATCATTTTTTTTACTTAGTAAACCTTCTAAATATTAGTATTAAAATTATTAATTAATTGTACCACGTAAACCATAAAATTAAGACTTCTTTCTTTTCGAGTTCAACAATATTTAGGTTGAGAAATTCAAATTTCTTGATTGAAGATATATGTCTTAACCAGTTGAGTTATGTTTAAATTGACACTTCATCTGAAGTTTTTAAATTTATCGAGGATGTATAATCTTAATTCGTCTAGTTATGAATGGTGTAGGAGCTATTACAAGTGTTCACATCAAGGATGCAAAGTGAAGAAACAAATTCAACGGCTTACAAATGACGAAGGAGTGGTACAAACAACCTACGAAGGAGTTCATTCCCATCCCATTGAAAAGCCTCAAGATAACTTTCAACATATCTTGACCCACATGCAAATCTACCCTTCTTCCTTTTAAAACCTTTTTTTCATTTTAATTTTTATTTATTTATTCACATTTTACACTTTTCAATTTTTATTATCAACTTCAATTCCCTCATTTCTTTGTCTATATAATAAGCCCTTTGCTTTTAGTATTTTAAACACATTTCCAATTTTGTATCTATTATATACTCAATACTCATTTTTAAGTGCGGTCCATTCCTTATCTTCATTCTTAATAAATGGGTCGAAAAAAGAAAATTTTATT

mRNA sequence

TTCAGCCTTATAAACTACTCCAATTAATTAAACCCTCAGATTGAGATTAATATTAATATGGATAATAATTACCAAATCTTCTTCCCAGATTCCTCCTCCTCTCATAATTCACAGCCTACTACGCCCATGGATCATGATCAGTTCGAAGAAGAAGAAGATCAAGAAGAAGAAGAAAGTAGTGTGAAGAAAACAAGTGGATCAGGGAAATCGAAGAATGAGAAGATAAGGAAAGGAAGATTTGCGTTTGAAACCAGGAGCCAAGTTGATGTGCTTGACGATGGCTATCGCTGGAGAAAATATGGTCAAAAGGCTGTTAAGAACAACAAGTTTCCCAGGAGCTATTACAAGTGTTCACATCAAGGATGCAAAGTGAAGAAACAAATTCAACGGCTTACAAATGACGAAGGAGTGGTACAAACAACCTACGAAGGAGTTCATTCCCATCCCATTGAAAAGCCTCAAGATAACTTTCAACATATCTTGACCCACATGCAAATCTACCCTTCTTCCTTTTAAAACCTTTTTTTCATTTTAATTTTTATTTATTTATTCACATTTTACACTTTTCAATTTTTATTATCAACTTCAATTCCCTCATTTCTTTGTCTATATAATAAGCCCTTTGCTTTTAGTATTTTAAACACATTTCCAATTTTGTATCTATTATATACTCAATACTCATTTTTAAGTGCGGTCCATTCCTTATCTTCATTCTTAATAAATGGGTCGAAAAAAGAAAATTTTATT

Coding sequence (CDS)

ATGGATAATAATTACCAAATCTTCTTCCCAGATTCCTCCTCCTCTCATAATTCACAGCCTACTACGCCCATGGATCATGATCAGTTCGAAGAAGAAGAAGATCAAGAAGAAGAAGAAAGTAGTGTGAAGAAAACAAGTGGATCAGGGAAATCGAAGAATGAGAAGATAAGGAAAGGAAGATTTGCGTTTGAAACCAGGAGCCAAGTTGATGTGCTTGACGATGGCTATCGCTGGAGAAAATATGGTCAAAAGGCTGTTAAGAACAACAAGTTTCCCAGGAGCTATTACAAGTGTTCACATCAAGGATGCAAAGTGAAGAAACAAATTCAACGGCTTACAAATGACGAAGGAGTGGTACAAACAACCTACGAAGGAGTTCATTCCCATCCCATTGAAAAGCCTCAAGATAACTTTCAACATATCTTGACCCACATGCAAATCTACCCTTCTTCCTTTTAA

Protein sequence

MDNNYQIFFPDSSSSHNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGKSKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPSSF

Homology

[Close]

BLAST of Lsi03G004080 vs. Swiss-Prot
Match: WRK75_ARATH (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana GN=WRKY75 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 6.0e-39
Identity = 89/151 (58.94%), Postives = 107/151 (70.86%), Query Frame = 1


Query: 3   NNYQIFFPDSSSSHNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGKSKNEKIRKG--- 62
           +N  ++ P  S   +S+P           E  Q EEESS  ++ G  KS     +KG   
Sbjct: 5   DNGSLYAPFLSLKSHSKP-----------ELHQGEEESSKVRSEGCSKSVESSKKKGKKQ 64

Query: 63  RFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVV 122
           R+AF+TRSQVD+LDDGYRWRKYGQKAVKNNKFPRSYY+C++ GC VKKQ+QRLT D+ VV
Sbjct: 65  RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVV 124

Query: 123 QTTYEGVHSHPIEKPQDNFQHILTHMQIYPS 151
            TTYEGVHSHPIEK  +NF+HILT MQIY S
Sbjct: 125 VTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of Lsi03G004080 vs. Swiss-Prot
Match: WRK45_ARATH (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana GN=WRKY45 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 9.6e-37
Identity = 84/159 (52.83%), Postives = 103/159 (64.78%), Query Frame = 1


Query: 2   DNNYQIFFPDSSS-----------SHNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGK 61
           D    + FP SSS            +++QPTT             EE+  S KK      
Sbjct: 3   DRRCDVLFPCSSSVDPRLTEFHGVDNSAQPTT-----------SSEEKPRSKKK------ 62

Query: 62  SKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQ 121
              +K R+ R+AF+TRSQVD+LDDGYRWRKYGQKAVKNN FPRSYYKC+ +GC+VKKQ+Q
Sbjct: 63  ---KKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEEGCRVKKQVQ 122

Query: 122 RLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYP 150
           R   DEGVV TTY+GVH+H ++KP DNF HILT M I+P
Sbjct: 123 RQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIFP 141

[Close]

BLAST of Lsi03G004080 vs. Swiss-Prot
Match: WRK43_ARATH (Probable WRKY transcription factor 43 OS=Arabidopsis thaliana GN=WRKY43 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.6e-26
Identity = 50/96 (52.08%), Postives = 73/96 (76.04%), Query Frame = 1


Query: 51  SKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQ 110
           S+++K++  RF+F T+S  D+LDDGYRWRKYGQK+VKN+ +PRSYY+C+   C VKKQ+Q
Sbjct: 8   SRDKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQ 67

Query: 111 RLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQ 147
           RL+ +  +V+TTYEG+H+HP E+       +L  +Q
Sbjct: 68  RLSKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQ 103

[Close]

BLAST of Lsi03G004080 vs. Swiss-Prot
Match: WRK56_ARATH (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana GN=WRKY56 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.6e-26
Identity = 60/107 (56.07%), Postives = 70/107 (65.42%), Query Frame = 1


Query: 40  SSVKKTSGSGKSKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCS 99
           S   K    GK K   +   R AF TRS  DVLDDGYRWRKYGQK+VKNN  PRSYY+C+
Sbjct: 82  SDHNKNCNKGKGKRT-LAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCT 141

Query: 100 HQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQ 147
           +  C VKKQ+QRL  D  VV TTYEGVH+HP EK  +    +L  +Q
Sbjct: 142 YHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

[Close]

BLAST of Lsi03G004080 vs. Swiss-Prot
Match: WRK24_ARATH (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana GN=WRKY24 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.9e-25
Identity = 69/147 (46.94%), Postives = 85/147 (57.82%), Query Frame = 1


Query: 10  PDSSSS------HNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGKSKNEKIRKGRFAF 69
           P SSSS      H   P  P ++DQ  E+  + +E+ S K                R AF
Sbjct: 43  PSSSSSWYPESFHVHAPPLPPENDQIGEKGKELKEKRSRKVP--------------RIAF 102

Query: 70  ETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVVQTTY 129
            TRS  DVLDDGYRWRKYGQK+VK+N  PRSYY+C++  C VKKQ+QRL  D  VV TTY
Sbjct: 103 HTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTY 162

Query: 130 EGVHSHPIEKPQDNFQHILTHMQIYPS 151
           EGVH+HP EK  +    +L  +Q   S
Sbjct: 163 EGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of Lsi03G004080 vs. TrEMBL
Match: A0A0A0LT01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406060 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 6.5e-48
Identity = 102/126 (80.95%), Postives = 110/126 (87.30%), Query Frame = 1


Query: 30  EEEEDQEEEESSVKKT----SGSGKSKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKA 89
           EEE+D+ EEE S+KK     SG    K +KIRK RFAFETRSQVDVLDDGYRWRKYGQKA
Sbjct: 3   EEEDDELEEERSLKKVKSEESGGELKKKKKIRKRRFAFETRSQVDVLDDGYRWRKYGQKA 62

Query: 90  VKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHM 149
           VKNNKFPRSYYKCS++GCKVKKQIQRLTNDEGVV TTYEGVHSHPIEKP D+FQ+ILTHM
Sbjct: 63  VKNNKFPRSYYKCSNEGCKVKKQIQRLTNDEGVVLTTYEGVHSHPIEKPHDSFQNILTHM 122

Query: 150 QIYPSS 152
            IYPSS
Sbjct: 123 HIYPSS 128

[Close]

BLAST of Lsi03G004080 vs. TrEMBL
Match: A0A0A0KU50_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G012390 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.1e-42
Identity = 85/120 (70.83%), Postives = 99/120 (82.50%), Query Frame = 1


Query: 39  ESSVKKTSGSGKSKNE------KIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFP 98
           E  +KK  G G+ + E      K+R  RFAF+TRSQVD+LDDGYRWRKYGQKAVKNNKFP
Sbjct: 96  EYGLKKEEGEGRGRGEDCDKKKKMRNRRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFP 155

Query: 99  RSYYKCSHQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPSSF 153
           RSYY+C+HQGCKVKKQ+QRLT DEGVV TTYEG+HSHPIEK  DNF+HIL+ MQIY +S+
Sbjct: 156 RSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIEKSTDNFEHILSQMQIYTTSY 215

[Close]

BLAST of Lsi03G004080 vs. TrEMBL
Match: A0A068UY66_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00038276001 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.3e-42
Identity = 84/106 (79.25%), Postives = 96/106 (90.57%), Query Frame = 1


Query: 48  SGKSK-NEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVK 107
           SGK K ++KIRK R+AF+TRSQVD+LDDGYRWRKYGQKAVKNNKFPRSYY+C+HQGC VK
Sbjct: 86  SGKKKADKKIRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVK 145

Query: 108 KQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPSSF 153
           KQ+QRL+ DEGVV TTYEG+HSHPIEK  DNF+HILT MQIY +SF
Sbjct: 146 KQVQRLSKDEGVVVTTYEGMHSHPIEKSTDNFEHILTQMQIYTTSF 191

[Close]

BLAST of Lsi03G004080 vs. TrEMBL
Match: A0A151QUC9_CAJCA (Putative WRKY transcription factor 75 OS=Cajanus cajan GN=KK1_045166 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 9.0e-42
Identity = 83/104 (79.81%), Postives = 94/104 (90.38%), Query Frame = 1


Query: 46  SGSGKSKNEK-IRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCK 105
           SG+GK K EK +RK R+AF+TRSQVD+LDDGYRWRKYGQKAVKNNKFPRSYY+C+HQGC 
Sbjct: 23  SGNGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCN 82

Query: 106 VKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIY 149
           VKKQ+QRLT DEGVV TTYEGVH+HPIEK  DNF+HIL+ MQIY
Sbjct: 83  VKKQVQRLTKDEGVVVTTYEGVHTHPIEKTTDNFEHILSQMQIY 126

[Close]

BLAST of Lsi03G004080 vs. TrEMBL
Match: A0A0D2SI88_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G164300 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.2e-41
Identity = 100/173 (57.80%), Postives = 122/173 (70.52%), Query Frame = 1


Query: 4   NYQIFFPDSSSSHNSQP---TTPMDHDQFEEEEDQE----------------EEESSVKK 63
           N+Q FFP SSSS  S     T+ +D+ +FE  +D +                E  S V  
Sbjct: 3   NFQGFFPTSSSSSTSLSLNLTSSLDYSEFECGKDNDGILGLIADIGVGGAKNENNSLVGT 62

Query: 64  TSGS----GKSK---NEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYK 123
           T+ S    GK+K   N+KIRK R+AF+TRSQVD+LDDGYRWRKYG KAVKN+KFPRSYY+
Sbjct: 63  TTESEVKSGKTKKGENKKIRKPRYAFQTRSQVDILDDGYRWRKYGHKAVKNSKFPRSYYR 122

Query: 124 CSHQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPS 151
           C+HQGCKVKKQ+QRLT DEG+V+TTYEG+HSHPI+K  DNF+HIL  MQIY S
Sbjct: 123 CTHQGCKVKKQVQRLTKDEGIVETTYEGMHSHPIQKTNDNFEHILNQMQIYTS 175

[Close]

BLAST of Lsi03G004080 vs. TAIR10
Match: AT5G13080.1 (AT5G13080.1 WRKY DNA-binding protein 75)

HSP 1 Score: 161.8 bits (408), Expect = 3.4e-40
Identity = 89/151 (58.94%), Postives = 107/151 (70.86%), Query Frame = 1


Query: 3   NNYQIFFPDSSSSHNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGKSKNEKIRKG--- 62
           +N  ++ P  S   +S+P           E  Q EEESS  ++ G  KS     +KG   
Sbjct: 5   DNGSLYAPFLSLKSHSKP-----------ELHQGEEESSKVRSEGCSKSVESSKKKGKKQ 64

Query: 63  RFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVV 122
           R+AF+TRSQVD+LDDGYRWRKYGQKAVKNNKFPRSYY+C++ GC VKKQ+QRLT D+ VV
Sbjct: 65  RYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVV 124

Query: 123 QTTYEGVHSHPIEKPQDNFQHILTHMQIYPS 151
            TTYEGVHSHPIEK  +NF+HILT MQIY S
Sbjct: 125 VTTYEGVHSHPIEKSTENFEHILTQMQIYSS 144

[Close]

BLAST of Lsi03G004080 vs. TAIR10
Match: AT3G01970.1 (AT3G01970.1 WRKY DNA-binding protein 45)

HSP 1 Score: 154.5 bits (389), Expect = 5.4e-38
Identity = 84/159 (52.83%), Postives = 103/159 (64.78%), Query Frame = 1


Query: 2   DNNYQIFFPDSSS-----------SHNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGK 61
           D    + FP SSS            +++QPTT             EE+  S KK      
Sbjct: 3   DRRCDVLFPCSSSVDPRLTEFHGVDNSAQPTT-----------SSEEKPRSKKK------ 62

Query: 62  SKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQ 121
              +K R+ R+AF+TRSQVD+LDDGYRWRKYGQKAVKNN FPRSYYKC+ +GC+VKKQ+Q
Sbjct: 63  ---KKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEEGCRVKKQVQ 122

Query: 122 RLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYP 150
           R   DEGVV TTY+GVH+H ++KP DNF HILT M I+P
Sbjct: 123 RQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIFP 141

[Close]

BLAST of Lsi03G004080 vs. TAIR10
Match: AT2G46130.1 (AT2G46130.1 WRKY DNA-binding protein 43)

HSP 1 Score: 119.8 bits (299), Expect = 1.5e-27
Identity = 50/96 (52.08%), Postives = 73/96 (76.04%), Query Frame = 1


Query: 51  SKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQ 110
           S+++K++  RF+F T+S  D+LDDGYRWRKYGQK+VKN+ +PRSYY+C+   C VKKQ+Q
Sbjct: 8   SRDKKMKNPRFSFRTKSDADILDDGYRWRKYGQKSVKNSLYPRSYYRCTQHMCNVKKQVQ 67

Query: 111 RLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQ 147
           RL+ +  +V+TTYEG+H+HP E+       +L  +Q
Sbjct: 68  RLSKETSIVETTYEGIHNHPCEELMQTLTPLLHQLQ 103

[Close]

BLAST of Lsi03G004080 vs. TAIR10
Match: AT1G64000.1 (AT1G64000.1 WRKY DNA-binding protein 56)

HSP 1 Score: 119.8 bits (299), Expect = 1.5e-27
Identity = 60/107 (56.07%), Postives = 70/107 (65.42%), Query Frame = 1


Query: 40  SSVKKTSGSGKSKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCS 99
           S   K    GK K   +   R AF TRS  DVLDDGYRWRKYGQK+VKNN  PRSYY+C+
Sbjct: 82  SDHNKNCNKGKGKRT-LAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCT 141

Query: 100 HQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQ 147
           +  C VKKQ+QRL  D  VV TTYEGVH+HP EK  +    +L  +Q
Sbjct: 142 YHTCNVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

[Close]

BLAST of Lsi03G004080 vs. TAIR10
Match: AT5G41570.1 (AT5G41570.1 WRKY DNA-binding protein 24)

HSP 1 Score: 116.3 bits (290), Expect = 1.6e-26
Identity = 69/147 (46.94%), Postives = 85/147 (57.82%), Query Frame = 1


Query: 10  PDSSSS------HNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGSGKSKNEKIRKGRFAF 69
           P SSSS      H   P  P ++DQ  E+  + +E+ S K                R AF
Sbjct: 43  PSSSSSWYPESFHVHAPPLPPENDQIGEKGKELKEKRSRKVP--------------RIAF 102

Query: 70  ETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVVQTTY 129
            TRS  DVLDDGYRWRKYGQK+VK+N  PRSYY+C++  C VKKQ+QRL  D  VV TTY
Sbjct: 103 HTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTCNVKKQVQRLAKDPNVVVTTY 162

Query: 130 EGVHSHPIEKPQDNFQHILTHMQIYPS 151
           EGVH+HP EK  +    +L  +Q   S
Sbjct: 163 EGVHNHPCEKLMETLNPLLRQLQFLSS 175

[Close]

BLAST of Lsi03G004080 vs. NCBI nr
Match: gi|778673213|ref|XP_004138624.2| (PREDICTED: probable WRKY transcription factor 45 [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 9.3e-48
Identity = 102/126 (80.95%), Postives = 110/126 (87.30%), Query Frame = 1


Query: 30  EEEEDQEEEESSVKKT----SGSGKSKNEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKA 89
           EEE+D+ EEE S+KK     SG    K +KIRK RFAFETRSQVDVLDDGYRWRKYGQKA
Sbjct: 3   EEEDDELEEERSLKKVKSEESGGELKKKKKIRKRRFAFETRSQVDVLDDGYRWRKYGQKA 62

Query: 90  VKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHM 149
           VKNNKFPRSYYKCS++GCKVKKQIQRLTNDEGVV TTYEGVHSHPIEKP D+FQ+ILTHM
Sbjct: 63  VKNNKFPRSYYKCSNEGCKVKKQIQRLTNDEGVVLTTYEGVHSHPIEKPHDSFQNILTHM 122

Query: 150 QIYPSS 152
            IYPSS
Sbjct: 123 HIYPSS 128

[Close]

BLAST of Lsi03G004080 vs. NCBI nr
Match: gi|659081408|ref|XP_008441319.1| (PREDICTED: probable WRKY transcription factor 75 [Cucumis melo])

HSP 1 Score: 194.1 bits (492), Expect = 1.7e-46
Identity = 107/153 (69.93%), Postives = 116/153 (75.82%), Query Frame = 1


Query: 1   MDNNYQIFFPDSSSSHNSQPTTPMDHDQFEEEEDQEEEESSVKKTSGS---GKSKNEKIR 60
           MD++Y  F P SSSS         DHD      D+ EEESS+KK       GK K +K R
Sbjct: 1   MDSSYINFLPTSSSSFFHNSLAMDDHD------DELEEESSLKKIKSEVSGGKLKKKKTR 60

Query: 61  KGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVKKQIQRLTNDEG 120
           K RFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCS++GCKVKKQIQRLT DE 
Sbjct: 61  KRRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSNEGCKVKKQIQRLTKDEE 120

Query: 121 VVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPS 151
           VV TTYEGVHSHPIEKP D+FQ+ILTHM IY S
Sbjct: 121 VVLTTYEGVHSHPIEKPHDSFQNILTHMHIYSS 147

[Close]

BLAST of Lsi03G004080 vs. NCBI nr
Match: gi|778689952|ref|XP_004152302.2| (PREDICTED: probable WRKY transcription factor 75 [Cucumis sativus])

HSP 1 Score: 179.5 bits (454), Expect = 4.5e-42
Identity = 85/120 (70.83%), Postives = 99/120 (82.50%), Query Frame = 1


Query: 39  ESSVKKTSGSGKSKNE------KIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFP 98
           E  +KK  G G+ + E      K+R  RFAF+TRSQVD+LDDGYRWRKYGQKAVKNNKFP
Sbjct: 96  EYGLKKEEGEGRGRGEDCDKKKKMRNRRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFP 155

Query: 99  RSYYKCSHQGCKVKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPSSF 153
           RSYY+C+HQGCKVKKQ+QRLT DEGVV TTYEG+HSHPIEK  DNF+HIL+ MQIY +S+
Sbjct: 156 RSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPIEKSTDNFEHILSQMQIYTTSY 215

[Close]

BLAST of Lsi03G004080 vs. NCBI nr
Match: gi|661883158|emb|CDP13366.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 178.7 bits (452), Expect = 7.6e-42
Identity = 84/106 (79.25%), Postives = 96/106 (90.57%), Query Frame = 1


Query: 48  SGKSK-NEKIRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCKVK 107
           SGK K ++KIRK R+AF+TRSQVD+LDDGYRWRKYGQKAVKNNKFPRSYY+C+HQGC VK
Sbjct: 86  SGKKKADKKIRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVK 145

Query: 108 KQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIYPSSF 153
           KQ+QRL+ DEGVV TTYEG+HSHPIEK  DNF+HILT MQIY +SF
Sbjct: 146 KQVQRLSKDEGVVVTTYEGMHSHPIEKSTDNFEHILTQMQIYTTSF 191

[Close]

BLAST of Lsi03G004080 vs. NCBI nr
Match: gi|1012321421|gb|KYP33931.1| (putative WRKY transcription factor 75 [Cajanus cajan])

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-41
Identity = 83/104 (79.81%), Postives = 94/104 (90.38%), Query Frame = 1


Query: 46  SGSGKSKNEK-IRKGRFAFETRSQVDVLDDGYRWRKYGQKAVKNNKFPRSYYKCSHQGCK 105
           SG+GK K EK +RK R+AF+TRSQVD+LDDGYRWRKYGQKAVKNNKFPRSYY+C+HQGC 
Sbjct: 23  SGNGKKKGEKKVRKPRYAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCN 82

Query: 106 VKKQIQRLTNDEGVVQTTYEGVHSHPIEKPQDNFQHILTHMQIY 149
           VKKQ+QRLT DEGVV TTYEGVH+HPIEK  DNF+HIL+ MQIY
Sbjct: 83  VKKQVQRLTKDEGVVVTTYEGVHTHPIEKTTDNFEHILSQMQIY 126

The following BLAST results are available for this feature:

BLAST of Lsi03G004080 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WRK75_ARATH	6.0e-39	58.94	Probable WRKY transcription factor 75 OS=Arabidopsis thaliana GN=WRKY75 PE=2 SV=...	[more]
WRK45_ARATH	9.6e-37	52.83	Probable WRKY transcription factor 45 OS=Arabidopsis thaliana GN=WRKY45 PE=2 SV=...	[more]
WRK43_ARATH	2.6e-26	52.08	Probable WRKY transcription factor 43 OS=Arabidopsis thaliana GN=WRKY43 PE=1 SV=...	[more]
WRK56_ARATH	2.6e-26	56.07	Probable WRKY transcription factor 56 OS=Arabidopsis thaliana GN=WRKY56 PE=2 SV=...	[more]
WRK24_ARATH	2.9e-25	46.94	Probable WRKY transcription factor 24 OS=Arabidopsis thaliana GN=WRKY24 PE=2 SV=...	[more]

BLAST of Lsi03G004080 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT01_CUCSA	6.5e-48	80.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G406060 PE=4 SV=1	[more]
A0A0A0KU50_CUCSA	3.1e-42	70.83	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G012390 PE=4 SV=1	[more]
A0A068UY66_COFCA	5.3e-42	79.25	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00038276001 PE=4 SV=1	[more]
A0A151QUC9_CAJCA	9.0e-42	79.81	Putative WRKY transcription factor 75 OS=Cajanus cajan GN=KK1_045166 PE=4 SV=1	[more]
A0A0D2SI88_GOSRA	1.2e-41	57.80	Uncharacterized protein OS=Gossypium raimondii GN=B456_005G164300 PE=4 SV=1	[more]

BLAST of Lsi03G004080 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G13080.1	3.4e-40	58.94	WRKY DNA-binding protein 75	[more]
AT3G01970.1	5.4e-38	52.83	WRKY DNA-binding protein 45	[more]
AT2G46130.1	1.5e-27	52.08	WRKY DNA-binding protein 43	[more]
AT1G64000.1	1.5e-27	56.07	WRKY DNA-binding protein 56	[more]
AT5G41570.1	1.6e-26	46.94	WRKY DNA-binding protein 24	[more]

BLAST of Lsi03G004080 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778673213\|ref\|XP_004138624.2\|	9.3e-48	80.95	PREDICTED: probable WRKY transcription factor 45 [Cucumis sativus]	[more]
gi\|659081408\|ref\|XP_008441319.1\|	1.7e-46	69.93	PREDICTED: probable WRKY transcription factor 75 [Cucumis melo]	[more]
gi\|778689952\|ref\|XP_004152302.2\|	4.5e-42	70.83	PREDICTED: probable WRKY transcription factor 75 [Cucumis sativus]	[more]
gi\|661883158\|emb\|CDP13366.1\|	7.6e-42	79.25	unnamed protein product [Coffea canephora]	[more]
gi\|1012321421\|gb\|KYP33931.1\|	1.3e-41	79.81	putative WRKY transcription factor 75 [Cajanus cajan]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0043565	sequence-specific DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

Vocabulary: **INTERPRO**
Term	Definition
IPR003657	WRKY_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010055	atrichoblast differentiation
biological_process	GO:0048527	lateral root development
biological_process	GO:0000122	negative regulation of transcription from RNA polymerase II promoter
biological_process	GO:0043620	regulation of DNA-templated transcription in response to stress
biological_process	GO:0032107	regulation of response to nutrient levels
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0001046	core promoter sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G004080.1	Lsi03G004080.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	GENE3D	G3DSA:2.20.25.80		coord: 57..130 score: 3.7
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 73..130 score: 5.0
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 72..131 score: 1.0
IPR003657	WRKY domain	PROFILE	PS50811	WRKY	coord: 67..132 score: 28
IPR003657	WRKY domain	unknown	SSF118290	WRKY DNA-binding domain	coord: 65..131 score: 7.32
None	No IPR available	PANTHER	PTHR31221	FAMILY NOT NAMED	coord: 17..150 score: 2.2
None	No IPR available	PANTHER	PTHR31221:SF25	WRKY TRANSCRIPTION FACTOR 45-RELATED	coord: 17..150 score: 2.2

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi03G004080	Csa2G406060	Cucumber (Chinese Long) v2	culsiB115
Lsi03G004080	CSPI02G25500	Wild cucumber (PI 183967)	cpilsiB113
Lsi03G004080	Cucsa.087850	Cucumber (Gy14) v1	cgylsiB098
Lsi03G004080	Cla003370	Watermelon (97103) v1	lsiwmB279
Lsi03G004080	MELO3C008175	Melon (DHL92) v3.5.1	lsimeB259
Lsi03G004080	ClCG11G009430	Watermelon (Charleston Gray)	lsiwcgB229
Lsi03G004080	MELO3C008175.2	Melon (DHL92) v3.6.1	lsimedB272

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi03G004080	Watermelon (97103) v2	lsiwmbB223
Lsi03G004080	Wax gourd	lsiwgoB322
Lsi03G004080	Cucurbita maxima (Rimu)	cmalsiB276
Lsi03G004080	Cucurbita maxima (Rimu)	cmalsiB656
Lsi03G004080	Cucurbita moschata (Rifu)	cmolsiB265
Lsi03G004080	Cucurbita moschata (Rifu)	cmolsiB645
Lsi03G004080	Cucurbita pepo (Zucchini)	cpelsiB143
Lsi03G004080	Cucurbita pepo (Zucchini)	cpelsiB339
Lsi03G004080	Cucumber (Gy14) v2	cgyblsiB102
Lsi03G004080	Silver-seed gourd	carlsiB017
Lsi03G004080	Silver-seed gourd	carlsiB924
Lsi03G004080	Cucumber (Chinese Long) v3	cuclsiB127