Lsi02G017750 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G017750
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionCalcium-binding family protein
Locationchr02 : 23534449 .. 23534925 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

mRNA sequence

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

Coding sequence (CDS)

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

Protein sequence

MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS
Homology
[Close]
BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML44_ARATH (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 4.3e-24
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 1 

Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVG-VDLISEDLESLIGKSKIDLNEFLCLYESI 67
           DL+R+F+T+D+N DG V+++EL W+L+++G  +   ++LE ++GK  +DL+EFL  Y   
Sbjct: 10  DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRFYYDA 69

Query: 68  REKNEGDNRGNEEEIESD--LWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGG 127
              ++G  +  +   ++D  +  AF VFD N DG+IS +EL++VL RLG   + +     
Sbjct: 70  VLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKAWD-- 129

Query: 128 DYCRRMIGAFDMNLDGRLDFQEFKNMM 152
             C RMI   D NLDG +DF+EFKNM+
Sbjct: 130 --CGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]
BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.4e-24
Identity = 70/167 (41.92%), Postives = 101/167 (60.48%), Query Frame = 1 

Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  LQRIF+  D+NGDGF+++EEL+  L R+G++    DL+S +      G + ++ +
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 64  EFLCLYESIREKNEG---------DNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNV 123
           +F  L++++ +   G         D+  +  E ESDL  AF+VFDEN DGFIS  ELQ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 124 LVRLGLWNDERCDGGGDYCR--RMIGAFDMNLDGRLDFQEFKNMMNS 154
           L +LGL        GG+  R  +MI + D N DGR+DF EFKNMM +
Sbjct: 145 LKKLGL------PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

[Close]
BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML22_ORYSJ (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica GN=CML22 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.3e-22
Identity = 63/154 (40.91%), Postives = 88/154 (57.14%), Query Frame = 1 

Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLNEFLC 67
           +L R+FE  DRNGDG ++ EEL   L ++G+ + +++L ++I      G   +D+ EF  
Sbjct: 92  ELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGE 151

Query: 68  LYESIR----EKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 127
           LY SI     +  +G  +  EEE + D+  AF VFD N DG+I+ DEL  VL  LGL   
Sbjct: 152 LYRSIMAGGDDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQG 211

Query: 128 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
                  + CRRMIG  D + DGR+DF EF  MM
Sbjct: 212 RT----AEECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]
BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML3_ARATH (Calmodulin-like protein 3 OS=Arabidopsis thaliana GN=CML3 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 9.1e-22
Identity = 60/154 (38.96%), Postives = 90/154 (58.44%), Query Frame = 1 

Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           ++  +L RIF+  DRNGDG ++ +ELN  L  +G+ +  +DL  +I      G   +D+ 
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 64  EFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 123
           EF  LY++I E         E + E D+  AF VFD+NRDGFI+ +EL++VL  LGL   
Sbjct: 61  EFGGLYQTIME---------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 120

Query: 124 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
              +     C+RMI   D++ DG ++F+EFK MM
Sbjct: 121 RTLED----CKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]
BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-21
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 1 

Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  L R+F+  D+N DGF+++EEL+  L+R+G+D    DL+S +       K+ +  +
Sbjct: 24  LNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFD 83

Query: 64  EFLCLYESIREK-NEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWN 123
           +F  L++++ E    G+    +   ESDL  AF VFDE+ DGFIS  ELQ VL +LGL  
Sbjct: 84  DFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPE 143

Query: 124 DERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNS 154
                G  +   +MI + D N DGR+DF EFKNMM +
Sbjct: 144 ----AGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQT 176

[Close]
BLAST of Lsi02G017750 vs. TrEMBL
Match: V4TCZ3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002706mg PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.4e-39
Identity = 93/170 (54.71%), Postives = 117/170 (68.82%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           M PL++ DLQRIF+ +D+NGD  VS+EELNWLL R+GV L  E+LES +GKS +DLNEFL
Sbjct: 1   MCPLHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFL 60

Query: 61  CLYESIREKN-------------EGDNRGNEEEIE-SDLWNAFEVFDENRDGFISCDELQ 120
             ++SI ++N              GD+  N+++ E SDL  AF VFD N DGFISC+ELQ
Sbjct: 61  FFWKSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDLNGDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           NVL RLGLW+++     G  C RMI  +D NLDG LDF+EFKNMM  S S
Sbjct: 121 NVLSRLGLWDEK----SGKDCTRMICMYDTNLDGVLDFEEFKNMMLLSKS 166

[Close]
BLAST of Lsi02G017750 vs. TrEMBL
Match: F6H0H4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01630 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 7.4e-39
Identity = 88/158 (55.70%), Postives = 109/158 (68.99%), Query Frame = 1 

Query: 2   SPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLC 61
           S L+S DL RIF  +DRNGDG VS+ ELNWLL RVGV    ++LESL+G + +D NEFL 
Sbjct: 4   SSLSSIDLHRIFHKLDRNGDGLVSLGELNWLLERVGVQYSLDELESLVGNTTLDFNEFLV 63

Query: 62  LYESIREKNEGDNRGNEEEIE--------SDLWNAFEVFDENRDGFISCDELQNVLVRLG 121
            YESI  + +G++   EEE++         DL  AF VFD N DGFI+CDELQ+VL RLG
Sbjct: 64  FYESISTEKKGESEVEEEEVKEEEEEEDVGDLAKAFRVFDLNGDGFITCDELQSVLSRLG 123

Query: 122 LWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +W +   +GGGD CR MI  +D N DG LDF+EFKNMM
Sbjct: 124 MWEE---NGGGD-CRSMIKVYDTNSDGVLDFEEFKNMM 157

[Close]
BLAST of Lsi02G017750 vs. TrEMBL
Match: A0A061FHR5_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_035222 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.7e-38
Identity = 90/162 (55.56%), Postives = 112/162 (69.14%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL+  DLQRIFE +D+NGDG VS+EELNWLL R+GV    E+L S +GK  +D NEFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  CLYESIREKN-EGDN-----RGNEEEI-----ESDLWNAFEVFDENRDGFISCDELQNVL 120
             Y+SI  +N +G N      G E+ +     +SDL  AF+VFD N DGFISC+ELQ+VL
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLVKAFKVFDLNGDGFISCEELQSVL 148

Query: 121 VRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           VRLGLW+    +  G  CR MI  +D NLDG +DF+EFKNMM
Sbjct: 149 VRLGLWD----ESSGKDCRNMICFYDTNLDGMVDFEEFKNMM 186

[Close]
BLAST of Lsi02G017750 vs. TrEMBL
Match: W9RRJ8_9ROSA (Putative calcium-binding protein CML44 OS=Morus notabilis GN=L484_003448 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 4.8e-38
Identity = 89/165 (53.94%), Postives = 115/165 (69.70%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVD--LIS-EDLESLIGKSKIDLN 60
           M  L++ DL RIF+ +D+NGDG VS++ELNWLL R+GVD  LIS  +LESL+GKS +  +
Sbjct: 1   MCSLSANDLNRIFKKLDKNGDGLVSVDELNWLLERIGVDIQLISLTELESLVGKSSLSFD 60

Query: 61  EFLCLYESIREKNEGDNRGNE-----------EEIESDLWNAFEVFDENRDGFISCDELQ 120
           EFL  Y+SI   N G+++G +           +  +SDL  AF+VFD N DGFISC+ELQ
Sbjct: 61  EFLFFYKSISNPNNGESKGGDIDQDQDHDHDHDGEDSDLVKAFKVFDINDDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +VL+RLGL  D     G D C+ MIGAFD N DG+LDF+EFKNMM
Sbjct: 121 SVLIRLGLMEDHN-SSGRDNCKSMIGAFDANSDGQLDFEEFKNMM 164

[Close]
BLAST of Lsi02G017750 vs. TrEMBL
Match: A4L9I0_GOSHI (Putative calcium-binding protein OS=Gossypium hirsutum PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.1e-37
Identity = 92/176 (52.27%), Postives = 112/176 (63.64%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL+  DL+RIFE +D+NGDG VS+EELNWLL R+GV    E+LESL+GK  +   EFL
Sbjct: 1   MSPLSKNDLRRIFEKLDKNGDGLVSLEELNWLLERIGVQFSLEELESLVGKPCLGFEEFL 60

Query: 61  CLYESIREKNEG-----DNRGNEEEI---------------ESDLWNAFEVFDENRDGFI 120
             Y+SI   N       D    EEEI               +SDL  AF+VFD N DGFI
Sbjct: 61  FFYDSISNHNTKIEAAFDEEEEEEEIRMVHGGGDDDDDDHGDSDLAKAFKVFDLNGDGFI 120

Query: 121 SCDELQNVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           SC+ELQ+VLVRLGLW+++     G  CR MI  +D NLDG +DF+EFKNMM  S S
Sbjct: 121 SCEELQSVLVRLGLWDEK----NGKDCRNMICFYDTNLDGMIDFEEFKNMMLRSVS 172

[Close]
BLAST of Lsi02G017750 vs. TAIR10
Match: AT1G21550.1 (AT1G21550.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 112.5 bits (280), Expect = 2.4e-25
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 1 

Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVG-VDLISEDLESLIGKSKIDLNEFLCLYESI 67
           DL+R+F+T+D+N DG V+++EL W+L+++G  +   ++LE ++GK  +DL+EFL  Y   
Sbjct: 10  DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRFYYDA 69

Query: 68  REKNEGDNRGNEEEIESD--LWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGG 127
              ++G  +  +   ++D  +  AF VFD N DG+IS +EL++VL RLG   + +     
Sbjct: 70  VLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKAWD-- 129

Query: 128 DYCRRMIGAFDMNLDGRLDFQEFKNMM 152
             C RMI   D NLDG +DF+EFKNM+
Sbjct: 130 --CGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]
BLAST of Lsi02G017750 vs. TAIR10
Match: AT4G20780.1 (AT4G20780.1 calmodulin like 42)

HSP 1 Score: 111.7 bits (278), Expect = 4.2e-25
Identity = 70/167 (41.92%), Postives = 101/167 (60.48%), Query Frame = 1 

Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  LQRIF+  D+NGDGF+++EEL+  L R+G++    DL+S +      G + ++ +
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 64  EFLCLYESIREKNEG---------DNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNV 123
           +F  L++++ +   G         D+  +  E ESDL  AF+VFDEN DGFIS  ELQ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 124 LVRLGLWNDERCDGGGDYCR--RMIGAFDMNLDGRLDFQEFKNMMNS 154
           L +LGL        GG+  R  +MI + D N DGR+DF EFKNMM +
Sbjct: 145 LKKLGL------PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

[Close]
BLAST of Lsi02G017750 vs. TAIR10
Match: AT3G07490.1 (AT3G07490.1 ARF-GAP domain 11)

HSP 1 Score: 104.8 bits (260), Expect = 5.1e-23
Identity = 60/154 (38.96%), Postives = 90/154 (58.44%), Query Frame = 1 

Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           ++  +L RIF+  DRNGDG ++ +ELN  L  +G+ +  +DL  +I      G   +D+ 
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 64  EFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 123
           EF  LY++I E         E + E D+  AF VFD+NRDGFI+ +EL++VL  LGL   
Sbjct: 61  EFGGLYQTIME---------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 120

Query: 124 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
              +     C+RMI   D++ DG ++F+EFK MM
Sbjct: 121 RTLED----CKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]
BLAST of Lsi02G017750 vs. TAIR10
Match: AT5G44460.1 (AT5G44460.1 calmodulin like 43)

HSP 1 Score: 104.0 bits (258), Expect = 8.7e-23
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 1 

Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  L R+F+  D+N DGF+++EEL+  L+R+G+D    DL+S +       K+ +  +
Sbjct: 24  LNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFD 83

Query: 64  EFLCLYESIREK-NEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWN 123
           +F  L++++ E    G+    +   ESDL  AF VFDE+ DGFIS  ELQ VL +LGL  
Sbjct: 84  DFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPE 143

Query: 124 DERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNS 154
                G  +   +MI + D N DGR+DF EFKNMM +
Sbjct: 144 ----AGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQT 176

[Close]
BLAST of Lsi02G017750 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 104.0 bits (258), Expect = 8.7e-23
Identity = 59/157 (37.58%), Postives = 94/157 (59.87%), Query Frame = 1 

Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLNEFLC 67
           +L+R+F+  D+NGDG ++ EELN  L  +G+ +  +DL  +I      G   +D++EF  
Sbjct: 65  ELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFES 124

Query: 68  LYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCD 127
           LY SI +++  D    EE    D+ +AF VFD++ DGFI+ +EL++V+  LGL   +  D
Sbjct: 125 LYSSIVDEHHNDGETEEE----DMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 184

Query: 128 GGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
           G    C++MI   D + DGR++++EF  MM     SS
Sbjct: 185 G----CKKMIMQVDADGDGRVNYKEFLQMMKGGGFSS 213

[Close]
BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|568837720|ref|XP_006472871.1| (PREDICTED: probable calcium-binding protein CML44 [Citrus sinensis])

HSP 1 Score: 169.1 bits (427), Expect = 6.3e-39
Identity = 93/170 (54.71%), Postives = 117/170 (68.82%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           M PL++ DLQRIF+ +D+NGD  VS+EELNWLL R+GV L  E+LES +GKS +DLNEFL
Sbjct: 1   MCPLHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFL 60

Query: 61  CLYESIREKN-------------EGDNRGNEEEIE-SDLWNAFEVFDENRDGFISCDELQ 120
             ++SI ++N              GD+  N+++ E SDL  AF VFD N DGFISC+ELQ
Sbjct: 61  FFWKSISKQNNNKVDDHEVKGEINGDDNENDDDGEISDLLEAFNVFDLNGDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           NVL RLGLW+++     G  C RMI  +D NLDG LDF+EFKNMM  S S
Sbjct: 121 NVLSRLGLWDEK----SGKDCTRMICMYDTNLDGVLDFEEFKNMMLLSKS 166

[Close]
BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|567883485|ref|XP_006434301.1| (hypothetical protein CICLE_v10002706mg [Citrus clementina])

HSP 1 Score: 169.1 bits (427), Expect = 6.3e-39
Identity = 93/170 (54.71%), Postives = 117/170 (68.82%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           M PL++ DLQRIF+ +D+NGD  VS+EELNWLL R+GV L  E+LES +GKS +DLNEFL
Sbjct: 1   MCPLHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFL 60

Query: 61  CLYESIREKN-------------EGDNRGNEEEIE-SDLWNAFEVFDENRDGFISCDELQ 120
             ++SI ++N              GD+  N+++ E SDL  AF VFD N DGFISC+ELQ
Sbjct: 61  FFWKSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDLNGDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           NVL RLGLW+++     G  C RMI  +D NLDG LDF+EFKNMM  S S
Sbjct: 121 NVLSRLGLWDEK----SGKDCTRMICMYDTNLDGVLDFEEFKNMMLLSKS 166

[Close]
BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|225459681|ref|XP_002285886.1| (PREDICTED: probable calcium-binding protein CML44 [Vitis vinifera])

HSP 1 Score: 168.3 bits (425), Expect = 1.1e-38
Identity = 88/158 (55.70%), Postives = 109/158 (68.99%), Query Frame = 1 

Query: 2   SPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLC 61
           S L+S DL RIF  +DRNGDG VS+ ELNWLL RVGV    ++LESL+G + +D NEFL 
Sbjct: 4   SSLSSIDLHRIFHKLDRNGDGLVSLGELNWLLERVGVQYSLDELESLVGNTTLDFNEFLV 63

Query: 62  LYESIREKNEGDNRGNEEEIE--------SDLWNAFEVFDENRDGFISCDELQNVLVRLG 121
            YESI  + +G++   EEE++         DL  AF VFD N DGFI+CDELQ+VL RLG
Sbjct: 64  FYESISTEKKGESEVEEEEVKEEEEEEDVGDLAKAFRVFDLNGDGFITCDELQSVLSRLG 123

Query: 122 LWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +W +   +GGGD CR MI  +D N DG LDF+EFKNMM
Sbjct: 124 MWEE---NGGGD-CRSMIKVYDTNSDGVLDFEEFKNMM 157

[Close]
BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|659081990|ref|XP_008441608.1| (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo])

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-38
Identity = 84/159 (52.83%), Postives = 116/159 (72.96%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP++S DL RIF+ +D+N DG + ++EL WLL+R+G+ L  E+LES + K  +D +EFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  CLYESIREKNEGDNRGN-----EEEIESD---LWNAFEVFDENRDGFISCDELQNVLVRL 120
             YESI ++N+G+ +G      +++ E D   ++ AF+VFD N DGFISCDEL+NVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 GLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
            LW+  R +   DYCR MI A+D NLDG+LDF+EFKNMM
Sbjct: 121 ELWDASRSE--VDYCRSMIQAYDTNLDGKLDFEEFKNMM 157

[Close]
BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|590599643|ref|XP_007019238.1| (Calcium-binding EF-hand family protein [Theobroma cacao])

HSP 1 Score: 166.0 bits (419), Expect = 5.3e-38
Identity = 90/162 (55.56%), Postives = 112/162 (69.14%), Query Frame = 1 

Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL+  DLQRIFE +D+NGDG VS+EELNWLL R+GV    E+L S +GK  +D NEFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  CLYESIREKN-EGDN-----RGNEEEI-----ESDLWNAFEVFDENRDGFISCDELQNVL 120
             Y+SI  +N +G N      G E+ +     +SDL  AF+VFD N DGFISC+ELQ+VL
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLVKAFKVFDLNGDGFISCEELQSVL 148

Query: 121 VRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           VRLGLW+    +  G  CR MI  +D NLDG +DF+EFKNMM
Sbjct: 149 VRLGLWD----ESSGKDCRNMICFYDTNLDGMVDFEEFKNMM 186

The following BLAST results are available for this feature:
BLAST of Lsi02G017750 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML44_ARATH4.3e-2440.82Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=... [more]
CML42_ARATH7.4e-2441.92Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1[more]
CML22_ORYSJ5.3e-2240.91Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica GN=CML22 ... [more]
CML3_ARATH9.1e-2238.96Calmodulin-like protein 3 OS=Arabidopsis thaliana GN=CML3 PE=2 SV=1[more]
CML43_ARATH1.5e-2140.76Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=... [more]
BLAST of Lsi02G017750 vs. TrEMBL
Match NameE-valueIdentityDescription
V4TCZ3_9ROSI4.4e-3954.71Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002706mg PE=4 SV=1[more]
F6H0H4_VITVI7.4e-3955.70Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01630 PE=4 SV=... [more]
A0A061FHR5_THECC3.7e-3855.56Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_035222 PE=4 SV=... [more]
W9RRJ8_9ROSA4.8e-3853.94Putative calcium-binding protein CML44 OS=Morus notabilis GN=L484_003448 PE=4 SV... [more]
A4L9I0_GOSHI3.1e-3752.27Putative calcium-binding protein OS=Gossypium hirsutum PE=4 SV=1[more]
BLAST of Lsi02G017750 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G21550.12.4e-2540.82 Calcium-binding EF-hand family protein[more]
AT4G20780.14.2e-2541.92 calmodulin like 42[more]
AT3G07490.15.1e-2338.96 ARF-GAP domain 11[more]
AT5G44460.18.7e-2340.76 calmodulin like 43[more]
AT2G43290.18.7e-2337.58 Calcium-binding EF-hand family protein[more]
BLAST of Lsi02G017750 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|568837720|ref|XP_006472871.1|6.3e-3954.71PREDICTED: probable calcium-binding protein CML44 [Citrus sinensis][more]
gi|567883485|ref|XP_006434301.1|6.3e-3954.71hypothetical protein CICLE_v10002706mg [Citrus clementina][more]
gi|225459681|ref|XP_002285886.1|1.1e-3855.70PREDICTED: probable calcium-binding protein CML44 [Vitis vinifera][more]
gi|659081990|ref|XP_008441608.1|1.8e-3852.83PREDICTED: probable calcium-binding protein CML44 [Cucumis melo][more]
gi|590599643|ref|XP_007019238.1|5.3e-3855.56Calcium-binding EF-hand family protein [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G017750.1Lsi02G017750.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 9..29
score: 5.
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 87..151
score: 2.
IPR002048EF-hand domainSMARTSM00054efh_1coord: 8..36
score: 1.6E-4coord: 84..112
score: 0.11coord: 126..154
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 4..39
score: 13.035coord: 80..115
score: 14.207coord: 122..157
score: 10
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 95..152
score: 2.2E-17coord: 8..94
score: 2.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 8..152
score: 1.24
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 93..105
score: -coord: 17..29
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..151
score: 2.6
NoneNo IPR availablePANTHERPTHR10891:SF530SUBFAMILY NOT NAMEDcoord: 1..151
score: 2.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G017750CmaCh09G010490Cucurbita maxima (Rimu)cmalsiB018
Lsi02G017750CmoCh09G010550Cucurbita moschata (Rifu)cmolsiB006
Lsi02G017750Cla005576Watermelon (97103) v1lsiwmB177
Lsi02G017750ClCG09G021840Watermelon (Charleston Gray)lsiwcgB161
Lsi02G017750Cla97C09G183290Watermelon (97103) v2lsiwmbB214
Lsi02G017750Bhi09G001558Wax gourdlsiwgoB277
Lsi02G017750Cp4.1LG06g08010Cucurbita pepo (Zucchini)cpelsiB633
Lsi02G017750Carg26621Silver-seed gourdcarlsiB942
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G017750Cucurbita maxima (Rimu)cmalsiB409
Lsi02G017750Cucurbita moschata (Rifu)cmolsiB395
Lsi02G017750Cucurbita pepo (Zucchini)cpelsiB445
Lsi02G017750Silver-seed gourdcarlsiB067
Lsi02G017750Wax gourdlsiwgoB280