Lsi02G017750 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G017750
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Calcium-binding family protein
Location	chr02 : 23534449 .. 23534925 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

mRNA sequence

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

Coding sequence (CDS)

ATGTCTCCATTGAACTCCTTCGATTTGCAGAGAATTTTCGAAACGATAGACAGAAATGGCGATGGCTTCGTGAGCATGGAGGAGCTCAATTGGCTGCTTAATCGTGTAGGCGTGGACCTAATTTCGGAGGATCTGGAGTCATTAATTGGTAAATCTAAGATTGATTTGAATGAATTTTTGTGTCTGTACGAATCGATAAGGGAGAAAAACGAGGGTGATAACAGGGGAAATGAGGAGGAGATTGAGAGCGATCTTTGGAATGCATTCGAAGTGTTCGACGAAAATCGTGATGGTTTCATTTCGTGCGATGAGCTTCAGAATGTGTTGGTGAGATTGGGGCTTTGGAATGATGAACGCTGTGATGGCGGTGGTGATTATTGTAGGCGTATGATCGGAGCGTTTGATATGAATTTGGATGGGCGGCTTGATTTTCAGGAATTCAAGAACATGATGAACTCTTCCAATTCTTCCTCTTGA

Protein sequence

MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS

Homology

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BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML44_ARATH (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 4.3e-24
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 1


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVG-VDLISEDLESLIGKSKIDLNEFLCLYESI 67
           DL+R+F+T+D+N DG V+++EL W+L+++G  +   ++LE ++GK  +DL+EFL  Y   
Sbjct: 10  DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRFYYDA 69

Query: 68  REKNEGDNRGNEEEIESD--LWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGG 127
              ++G  +  +   ++D  +  AF VFD N DG+IS +EL++VL RLG   + +     
Sbjct: 70  VLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKAWD-- 129

Query: 128 DYCRRMIGAFDMNLDGRLDFQEFKNMM 152
             C RMI   D NLDG +DF+EFKNM+
Sbjct: 130 --CGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]

BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML42_ARATH (Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 7.4e-24
Identity = 70/167 (41.92%), Postives = 101/167 (60.48%), Query Frame = 1


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  LQRIF+  D+NGDGF+++EEL+  L R+G++    DL+S +      G + ++ +
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 64  EFLCLYESIREKNEG---------DNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNV 123
           +F  L++++ +   G         D+  +  E ESDL  AF+VFDEN DGFIS  ELQ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 124 LVRLGLWNDERCDGGGDYCR--RMIGAFDMNLDGRLDFQEFKNMMNS 154
           L +LGL        GG+  R  +MI + D N DGR+DF EFKNMM +
Sbjct: 145 LKKLGL------PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

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BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML22_ORYSJ (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica GN=CML22 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.3e-22
Identity = 63/154 (40.91%), Postives = 88/154 (57.14%), Query Frame = 1


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLNEFLC 67
           +L R+FE  DRNGDG ++ EEL   L ++G+ + +++L ++I      G   +D+ EF  
Sbjct: 92  ELARVFELFDRNGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGE 151

Query: 68  LYESIR----EKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 127
           LY SI     +  +G  +  EEE + D+  AF VFD N DG+I+ DEL  VL  LGL   
Sbjct: 152 LYRSIMAGGDDSKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQG 211

Query: 128 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
                  + CRRMIG  D + DGR+DF EF  MM
Sbjct: 212 RT----AEECRRMIGQVDRDGDGRVDFHEFLQMM 241

[Close]

BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML3_ARATH (Calmodulin-like protein 3 OS=Arabidopsis thaliana GN=CML3 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 9.1e-22
Identity = 60/154 (38.96%), Postives = 90/154 (58.44%), Query Frame = 1


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           ++  +L RIF+  DRNGDG ++ +ELN  L  +G+ +  +DL  +I      G   +D+ 
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 64  EFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 123
           EF  LY++I E         E + E D+  AF VFD+NRDGFI+ +EL++VL  LGL   
Sbjct: 61  EFGGLYQTIME---------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 120

Query: 124 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
              +     C+RMI   D++ DG ++F+EFK MM
Sbjct: 121 RTLED----CKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi02G017750 vs. Swiss-Prot
Match: CML43_ARATH (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.5e-21
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 1


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  L R+F+  D+N DGF+++EEL+  L+R+G+D    DL+S +       K+ +  +
Sbjct: 24  LNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFD 83

Query: 64  EFLCLYESIREK-NEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWN 123
           +F  L++++ E    G+    +   ESDL  AF VFDE+ DGFIS  ELQ VL +LGL  
Sbjct: 84  DFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPE 143

Query: 124 DERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNS 154
                G  +   +MI + D N DGR+DF EFKNMM +
Sbjct: 144 ----AGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQT 176

[Close]

BLAST of Lsi02G017750 vs. TrEMBL
Match: V4TCZ3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002706mg PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.4e-39
Identity = 93/170 (54.71%), Postives = 117/170 (68.82%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           M PL++ DLQRIF+ +D+NGD  VS+EELNWLL R+GV L  E+LES +GKS +DLNEFL
Sbjct: 1   MCPLHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFL 60

Query: 61  CLYESIREKN-------------EGDNRGNEEEIE-SDLWNAFEVFDENRDGFISCDELQ 120
             ++SI ++N              GD+  N+++ E SDL  AF VFD N DGFISC+ELQ
Sbjct: 61  FFWKSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDLNGDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           NVL RLGLW+++     G  C RMI  +D NLDG LDF+EFKNMM  S S
Sbjct: 121 NVLSRLGLWDEK----SGKDCTRMICMYDTNLDGVLDFEEFKNMMLLSKS 166

[Close]

BLAST of Lsi02G017750 vs. TrEMBL
Match: F6H0H4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01630 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 7.4e-39
Identity = 88/158 (55.70%), Postives = 109/158 (68.99%), Query Frame = 1


Query: 2   SPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLC 61
           S L+S DL RIF  +DRNGDG VS+ ELNWLL RVGV    ++LESL+G + +D NEFL 
Sbjct: 4   SSLSSIDLHRIFHKLDRNGDGLVSLGELNWLLERVGVQYSLDELESLVGNTTLDFNEFLV 63

Query: 62  LYESIREKNEGDNRGNEEEIE--------SDLWNAFEVFDENRDGFISCDELQNVLVRLG 121
            YESI  + +G++   EEE++         DL  AF VFD N DGFI+CDELQ+VL RLG
Sbjct: 64  FYESISTEKKGESEVEEEEVKEEEEEEDVGDLAKAFRVFDLNGDGFITCDELQSVLSRLG 123

Query: 122 LWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +W +   +GGGD CR MI  +D N DG LDF+EFKNMM
Sbjct: 124 MWEE---NGGGD-CRSMIKVYDTNSDGVLDFEEFKNMM 157

[Close]

BLAST of Lsi02G017750 vs. TrEMBL
Match: A0A061FHR5_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_035222 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.7e-38
Identity = 90/162 (55.56%), Postives = 112/162 (69.14%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL+  DLQRIFE +D+NGDG VS+EELNWLL R+GV    E+L S +GK  +D NEFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  CLYESIREKN-EGDN-----RGNEEEI-----ESDLWNAFEVFDENRDGFISCDELQNVL 120
             Y+SI  +N +G N      G E+ +     +SDL  AF+VFD N DGFISC+ELQ+VL
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLVKAFKVFDLNGDGFISCEELQSVL 148

Query: 121 VRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           VRLGLW+    +  G  CR MI  +D NLDG +DF+EFKNMM
Sbjct: 149 VRLGLWD----ESSGKDCRNMICFYDTNLDGMVDFEEFKNMM 186

[Close]

BLAST of Lsi02G017750 vs. TrEMBL
Match: W9RRJ8_9ROSA (Putative calcium-binding protein CML44 OS=Morus notabilis GN=L484_003448 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 4.8e-38
Identity = 89/165 (53.94%), Postives = 115/165 (69.70%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVD--LIS-EDLESLIGKSKIDLN 60
           M  L++ DL RIF+ +D+NGDG VS++ELNWLL R+GVD  LIS  +LESL+GKS +  +
Sbjct: 1   MCSLSANDLNRIFKKLDKNGDGLVSVDELNWLLERIGVDIQLISLTELESLVGKSSLSFD 60

Query: 61  EFLCLYESIREKNEGDNRGNE-----------EEIESDLWNAFEVFDENRDGFISCDELQ 120
           EFL  Y+SI   N G+++G +           +  +SDL  AF+VFD N DGFISC+ELQ
Sbjct: 61  EFLFFYKSISNPNNGESKGGDIDQDQDHDHDHDGEDSDLVKAFKVFDINDDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +VL+RLGL  D     G D C+ MIGAFD N DG+LDF+EFKNMM
Sbjct: 121 SVLIRLGLMEDHN-SSGRDNCKSMIGAFDANSDGQLDFEEFKNMM 164

[Close]

BLAST of Lsi02G017750 vs. TrEMBL
Match: A4L9I0_GOSHI (Putative calcium-binding protein OS=Gossypium hirsutum PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.1e-37
Identity = 92/176 (52.27%), Postives = 112/176 (63.64%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL+  DL+RIFE +D+NGDG VS+EELNWLL R+GV    E+LESL+GK  +   EFL
Sbjct: 1   MSPLSKNDLRRIFEKLDKNGDGLVSLEELNWLLERIGVQFSLEELESLVGKPCLGFEEFL 60

Query: 61  CLYESIREKNEG-----DNRGNEEEI---------------ESDLWNAFEVFDENRDGFI 120
             Y+SI   N       D    EEEI               +SDL  AF+VFD N DGFI
Sbjct: 61  FFYDSISNHNTKIEAAFDEEEEEEEIRMVHGGGDDDDDDHGDSDLAKAFKVFDLNGDGFI 120

Query: 121 SCDELQNVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           SC+ELQ+VLVRLGLW+++     G  CR MI  +D NLDG +DF+EFKNMM  S S
Sbjct: 121 SCEELQSVLVRLGLWDEK----NGKDCRNMICFYDTNLDGMIDFEEFKNMMLRSVS 172

[Close]

BLAST of Lsi02G017750 vs. TAIR10
Match: AT1G21550.1 (AT1G21550.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 112.5 bits (280), Expect = 2.4e-25
Identity = 60/147 (40.82%), Postives = 95/147 (64.63%), Query Frame = 1


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVG-VDLISEDLESLIGKSKIDLNEFLCLYESI 67
           DL+R+F+T+D+N DG V+++EL W+L+++G  +   ++LE ++GK  +DL+EFL  Y   
Sbjct: 10  DLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRFYYDA 69

Query: 68  REKNEGDNRGNEEEIESD--LWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCDGGG 127
              ++G  +  +   ++D  +  AF VFD N DG+IS +EL++VL RLG   + +     
Sbjct: 70  VLDSKGSKKNIDVVADNDEAIARAFNVFDVNGDGYISAEELRDVLERLGFEEEAKAWD-- 129

Query: 128 DYCRRMIGAFDMNLDGRLDFQEFKNMM 152
             C RMI   D NLDG +DF+EFKNM+
Sbjct: 130 --CGRMIRVHDKNLDGFVDFEEFKNMI 152

[Close]

BLAST of Lsi02G017750 vs. TAIR10
Match: AT4G20780.1 (AT4G20780.1 calmodulin like 42)

HSP 1 Score: 111.7 bits (278), Expect = 4.2e-25
Identity = 70/167 (41.92%), Postives = 101/167 (60.48%), Query Frame = 1


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  LQRIF+  D+NGDGF+++EEL+  L R+G++    DL+S +      G + ++ +
Sbjct: 25  LNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNFD 84

Query: 64  EFLCLYESIREKNEG---------DNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNV 123
           +F  L++++ +   G         D+  +  E ESDL  AF+VFDEN DGFIS  ELQ V
Sbjct: 85  DFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTV 144

Query: 124 LVRLGLWNDERCDGGGDYCR--RMIGAFDMNLDGRLDFQEFKNMMNS 154
           L +LGL        GG+  R  +MI + D N DGR+DF EFKNMM +
Sbjct: 145 LKKLGL------PEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

[Close]

BLAST of Lsi02G017750 vs. TAIR10
Match: AT3G07490.1 (AT3G07490.1 ARF-GAP domain 11)

HSP 1 Score: 104.8 bits (260), Expect = 5.1e-23
Identity = 60/154 (38.96%), Postives = 90/154 (58.44%), Query Frame = 1


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           ++  +L RIF+  DRNGDG ++ +ELN  L  +G+ +  +DL  +I      G   +D+ 
Sbjct: 1   MDQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIE 60

Query: 64  EFLCLYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWND 123
           EF  LY++I E         E + E D+  AF VFD+NRDGFI+ +EL++VL  LGL   
Sbjct: 61  EFGGLYQTIME---------ERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQG 120

Query: 124 ERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
              +     C+RMI   D++ DG ++F+EFK MM
Sbjct: 121 RTLED----CKRMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi02G017750 vs. TAIR10
Match: AT5G44460.1 (AT5G44460.1 calmodulin like 43)

HSP 1 Score: 104.0 bits (258), Expect = 8.7e-23
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 1


Query: 4   LNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLN 63
           LN+  L R+F+  D+N DGF+++EEL+  L+R+G+D    DL+S +       K+ +  +
Sbjct: 24  LNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRFD 83

Query: 64  EFLCLYESIREK-NEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWN 123
           +F  L++++ E    G+    +   ESDL  AF VFDE+ DGFIS  ELQ VL +LGL  
Sbjct: 84  DFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPE 143

Query: 124 DERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNS 154
                G  +   +MI + D N DGR+DF EFKNMM +
Sbjct: 144 ----AGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQT 176

[Close]

BLAST of Lsi02G017750 vs. TAIR10
Match: AT2G43290.1 (AT2G43290.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 104.0 bits (258), Expect = 8.7e-23
Identity = 59/157 (37.58%), Postives = 94/157 (59.87%), Query Frame = 1


Query: 8   DLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLI------GKSKIDLNEFLC 67
           +L+R+F+  D+NGDG ++ EELN  L  +G+ +  +DL  +I      G   +D++EF  
Sbjct: 65  ELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFES 124

Query: 68  LYESIREKNEGDNRGNEEEIESDLWNAFEVFDENRDGFISCDELQNVLVRLGLWNDERCD 127
           LY SI +++  D    EE    D+ +AF VFD++ DGFI+ +EL++V+  LGL   +  D
Sbjct: 125 LYSSIVDEHHNDGETEEE----DMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 184

Query: 128 GGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNSSS 159
           G    C++MI   D + DGR++++EF  MM     SS
Sbjct: 185 G----CKKMIMQVDADGDGRVNYKEFLQMMKGGGFSS 213

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|568837720|ref|XP_006472871.1| (PREDICTED: probable calcium-binding protein CML44 [Citrus sinensis])

HSP 1 Score: 169.1 bits (427), Expect = 6.3e-39
Identity = 93/170 (54.71%), Postives = 117/170 (68.82%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           M PL++ DLQRIF+ +D+NGD  VS+EELNWLL R+GV L  E+LES +GKS +DLNEFL
Sbjct: 1   MCPLHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFL 60

Query: 61  CLYESIREKN-------------EGDNRGNEEEIE-SDLWNAFEVFDENRDGFISCDELQ 120
             ++SI ++N              GD+  N+++ E SDL  AF VFD N DGFISC+ELQ
Sbjct: 61  FFWKSISKQNNNKVDDHEVKGEINGDDNENDDDGEISDLLEAFNVFDLNGDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           NVL RLGLW+++     G  C RMI  +D NLDG LDF+EFKNMM  S S
Sbjct: 121 NVLSRLGLWDEK----SGKDCTRMICMYDTNLDGVLDFEEFKNMMLLSKS 166

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|567883485|ref|XP_006434301.1| (hypothetical protein CICLE_v10002706mg [Citrus clementina])

HSP 1 Score: 169.1 bits (427), Expect = 6.3e-39
Identity = 93/170 (54.71%), Postives = 117/170 (68.82%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           M PL++ DLQRIF+ +D+NGD  VS+EELNWLL R+GV L  E+LES +GKS +DLNEFL
Sbjct: 1   MCPLHTADLQRIFKQLDKNGDDQVSLEELNWLLERIGVRLSLEELESFVGKSSLDLNEFL 60

Query: 61  CLYESIREKN-------------EGDNRGNEEEIE-SDLWNAFEVFDENRDGFISCDELQ 120
             ++SI ++N              GD+  N+++ E SDL  AF VFD N DGFISC+ELQ
Sbjct: 61  FFWKSISKQNNNKVDDHEVKDEINGDDNENDDDGEISDLLEAFNVFDLNGDGFISCEELQ 120

Query: 121 NVLVRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMMNSSNS 157
           NVL RLGLW+++     G  C RMI  +D NLDG LDF+EFKNMM  S S
Sbjct: 121 NVLSRLGLWDEK----SGKDCTRMICMYDTNLDGVLDFEEFKNMMLLSKS 166

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|225459681|ref|XP_002285886.1| (PREDICTED: probable calcium-binding protein CML44 [Vitis vinifera])

HSP 1 Score: 168.3 bits (425), Expect = 1.1e-38
Identity = 88/158 (55.70%), Postives = 109/158 (68.99%), Query Frame = 1


Query: 2   SPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFLC 61
           S L+S DL RIF  +DRNGDG VS+ ELNWLL RVGV    ++LESL+G + +D NEFL 
Sbjct: 4   SSLSSIDLHRIFHKLDRNGDGLVSLGELNWLLERVGVQYSLDELESLVGNTTLDFNEFLV 63

Query: 62  LYESIREKNEGDNRGNEEEIE--------SDLWNAFEVFDENRDGFISCDELQNVLVRLG 121
            YESI  + +G++   EEE++         DL  AF VFD N DGFI+CDELQ+VL RLG
Sbjct: 64  FYESISTEKKGESEVEEEEVKEEEEEEDVGDLAKAFRVFDLNGDGFITCDELQSVLSRLG 123

Query: 122 LWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           +W +   +GGGD CR MI  +D N DG LDF+EFKNMM
Sbjct: 124 MWEE---NGGGD-CRSMIKVYDTNSDGVLDFEEFKNMM 157

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|659081990|ref|XP_008441608.1| (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo])

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-38
Identity = 84/159 (52.83%), Postives = 116/159 (72.96%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSP++S DL RIF+ +D+N DG + ++EL WLL+R+G+ L  E+LES + K  +D +EFL
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  CLYESIREKNEGDNRGN-----EEEIESD---LWNAFEVFDENRDGFISCDELQNVLVRL 120
             YESI ++N+G+ +G      +++ E D   ++ AF+VFD N DGFISCDEL+NVLVRL
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLVRL 120

Query: 121 GLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
            LW+  R +   DYCR MI A+D NLDG+LDF+EFKNMM
Sbjct: 121 ELWDASRSE--VDYCRSMIQAYDTNLDGKLDFEEFKNMM 157

[Close]

BLAST of Lsi02G017750 vs. NCBI nr
Match: gi|590599643|ref|XP_007019238.1| (Calcium-binding EF-hand family protein [Theobroma cacao])

HSP 1 Score: 166.0 bits (419), Expect = 5.3e-38
Identity = 90/162 (55.56%), Postives = 112/162 (69.14%), Query Frame = 1


Query: 1   MSPLNSFDLQRIFETIDRNGDGFVSMEELNWLLNRVGVDLISEDLESLIGKSKIDLNEFL 60
           MSPL+  DLQRIFE +D+NGDG VS+EELNWLL R+GV    E+L S +GK  +D NEFL
Sbjct: 29  MSPLSKNDLQRIFEKLDKNGDGLVSLEELNWLLVRIGVQFSLEELGSFVGKPCLDFNEFL 88

Query: 61  CLYESIREKN-EGDN-----RGNEEEI-----ESDLWNAFEVFDENRDGFISCDELQNVL 120
             Y+SI  +N +G N      G E+ +     +SDL  AF+VFD N DGFISC+ELQ+VL
Sbjct: 89  FFYDSISNQNDDGKNMEPAGSGEEDGLIQADEDSDLVKAFKVFDLNGDGFISCEELQSVL 148

Query: 121 VRLGLWNDERCDGGGDYCRRMIGAFDMNLDGRLDFQEFKNMM 152
           VRLGLW+    +  G  CR MI  +D NLDG +DF+EFKNMM
Sbjct: 149 VRLGLWD----ESSGKDCRNMICFYDTNLDGMVDFEEFKNMM 186

The following BLAST results are available for this feature:

BLAST of Lsi02G017750 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML44_ARATH	4.3e-24	40.82	Probable calcium-binding protein CML44 OS=Arabidopsis thaliana GN=CML44 PE=2 SV=...	[more]
CML42_ARATH	7.4e-24	41.92	Calcium-binding protein CML42 OS=Arabidopsis thaliana GN=CML42 PE=1 SV=1	[more]
CML22_ORYSJ	5.3e-22	40.91	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica GN=CML22 ...	[more]
CML3_ARATH	9.1e-22	38.96	Calmodulin-like protein 3 OS=Arabidopsis thaliana GN=CML3 PE=2 SV=1	[more]
CML43_ARATH	1.5e-21	40.76	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana GN=CML43 PE=2 SV=...	[more]

BLAST of Lsi02G017750 vs. TrEMBL


Match Name	E-value	Identity	Description
V4TCZ3_9ROSI	4.4e-39	54.71	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10002706mg PE=4 SV=1	[more]
F6H0H4_VITVI	7.4e-39	55.70	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g01630 PE=4 SV=...	[more]
A0A061FHR5_THECC	3.7e-38	55.56	Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_035222 PE=4 SV=...	[more]
W9RRJ8_9ROSA	4.8e-38	53.94	Putative calcium-binding protein CML44 OS=Morus notabilis GN=L484_003448 PE=4 SV...	[more]
A4L9I0_GOSHI	3.1e-37	52.27	Putative calcium-binding protein OS=Gossypium hirsutum PE=4 SV=1	[more]

BLAST of Lsi02G017750 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G21550.1	2.4e-25	40.82	Calcium-binding EF-hand family protein	[more]
AT4G20780.1	4.2e-25	41.92	calmodulin like 42	[more]
AT3G07490.1	5.1e-23	38.96	ARF-GAP domain 11	[more]
AT5G44460.1	8.7e-23	40.76	calmodulin like 43	[more]
AT2G43290.1	8.7e-23	37.58	Calcium-binding EF-hand family protein	[more]

BLAST of Lsi02G017750 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|568837720\|ref\|XP_006472871.1\|	6.3e-39	54.71	PREDICTED: probable calcium-binding protein CML44 [Citrus sinensis]	[more]
gi\|567883485\|ref\|XP_006434301.1\|	6.3e-39	54.71	hypothetical protein CICLE_v10002706mg [Citrus clementina]	[more]
gi\|225459681\|ref\|XP_002285886.1\|	1.1e-38	55.70	PREDICTED: probable calcium-binding protein CML44 [Vitis vinifera]	[more]
gi\|659081990\|ref\|XP_008441608.1\|	1.8e-38	52.83	PREDICTED: probable calcium-binding protein CML44 [Cucumis melo]	[more]
gi\|590599643\|ref\|XP_007019238.1\|	5.3e-38	55.56	Calcium-binding EF-hand family protein [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018247	EF_Hand_1_Ca_BS
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G017750.1	Lsi02G017750.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 9..29 score: 5.
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 87..151 score: 2.
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 8..36 score: 1.6E-4coord: 84..112 score: 0.11coord: 126..154 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 4..39 score: 13.035coord: 80..115 score: 14.207coord: 122..157 score: 10
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 95..152 score: 2.2E-17coord: 8..94 score: 2.6
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 8..152 score: 1.24
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 93..105 score: -coord: 17..29 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..151 score: 2.6
None	No IPR available	PANTHER	PTHR10891:SF530	SUBFAMILY NOT NAMED	coord: 1..151 score: 2.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi02G017750	CmaCh09G010490	Cucurbita maxima (Rimu)	cmalsiB018
Lsi02G017750	CmoCh09G010550	Cucurbita moschata (Rifu)	cmolsiB006
Lsi02G017750	Cla005576	Watermelon (97103) v1	lsiwmB177
Lsi02G017750	ClCG09G021840	Watermelon (Charleston Gray)	lsiwcgB161
Lsi02G017750	Cla97C09G183290	Watermelon (97103) v2	lsiwmbB214
Lsi02G017750	Bhi09G001558	Wax gourd	lsiwgoB277
Lsi02G017750	Cp4.1LG06g08010	Cucurbita pepo (Zucchini)	cpelsiB633
Lsi02G017750	Carg26621	Silver-seed gourd	carlsiB942

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi02G017750	Silver-seed gourd	carlsiB067
Lsi02G017750	Wax gourd	lsiwgoB280
Lsi02G017750	Cucurbita maxima (Rimu)	cmalsiB409
Lsi02G017750	Cucurbita moschata (Rifu)	cmolsiB395
Lsi02G017750	Cucurbita pepo (Zucchini)	cpelsiB445