Lsi02G010550 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi02G010550
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Glycine cleavage system H family protein
Location	chr02 : 11434685 .. 11437838 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGTTTGTTGTTGACACATTGACCCACCACCTTGCAGCTTCGACGTGGGATTGCTTTCGCAACCTCTATTTTTCTTCATTTCTCTTCCTTGTTTCAATTATTTTCAGATTAATGTTTATGATCTCACATCCCAAAAATAAAAAATGAAATGAAAATTGACCCAAAATACGTAAAATGCAAAGTATAAAACCCAAAAAGAAAAAAGAAATATATATATTATATATATGGTATACGAGCATCCCCACCAACCCTTCCATGAAACTGTTCTCTTAGAGCCGACGGCAAGCTAAAGCATTAGCAGCAAAACAAGCTTCTGTTGAGAAAAGGGTTTTTACAGAGCGCTCTAAGTTTCATCAATGGCGTCCAGGTTGGTTTGGGCTTCAAGAGCTGCTTCTTTTCTCAGGATCTCAATTCCCCTTAGAGGGTTTGCTTCTGGTGAGCCCAACCCATGTTTTTTTATCTACTGATTTTCCTTTTCTTTCTCAGTTTATATTAATGTTAAGCGAAAACAAGTGAAACTGATCGATCTGCTTTTGACATTGAAAGATCTCAGTTTCCCATGATGGGTTTTGTTTCTCTCTGTTGCTTGTTTGATCAATGCAGACCCTTTTCAAGAATTGCAGGATTTGTCTCGTTTTTTTGTATGGCTGTCACGTCTCATTGTCTTGTGAATGATTGAGGTGATTATATAATGAATATTTGTGGGTAGATCTTAGGCAGATGAATAAATGGCTTTTTTAAAAAACAAGAGAAACAAATTTTTCTTTTACTATCTTCTCTGTCTTTTGTCTCCCCTTTTCTCCCCCATTAATTATCTAATGAATATTTTGCCCATTCTTCTTTATGATTTTTTGAATTTTGAATCGGTTGTTGGGTTCACAGATGTTATTCTACTTATGAATCTACATCTGTTCATGTGCATTTGTTGAATATGCATATATTTTCACCTTCCATTTGAATTATGATGTGATGAATCTCAAACATGAAATATTCTATTCTATTATATTCTACTTGCCGTTTTCTCTCCTTCCTTTTATTTGTTTATGTGTTTTTATTTCAATCTTGTCATTTGAAATAACACCTGTGCCTTCATGAACTTTCTAGCTGCTGATATTGTTAATTATGTAGTTTTCTAGTTTTAATATCAATTTTCGTCCCTGGATTAACTCCATGCACATACCACTTTCCCAACAGTGTTTATCATTTTCAATAACTTTCTGTTTGTCTTCTATGACCAAAATGTAATAGTAATTTTCATTTCAAAGTTTGCACTTCATATTCTTGATATTTGCATCTACTAAACAATACAATCAAAGGGATGGAATGTAGAACTTAGAAGTATAAATCCATTTGAGAAGCAAAAGAAGCACTTGTACATACATTTGTACAATACACACTAGCATAGTCTTATGGAAAGGCAAGCTTTTTTTTTCTTGACAACATTTGGTGTTAGAGAATCGAACTTTTGACTTCGAGGTCGATAATACAAAGTTTATGCCGTTGAGCTATGCTCATTTTTGTTAGGAAGGCATGCATGTGATCATCTTACAAACGAAGAAAATTGAGAATAAAATGTTTCATACAGTCGCATGAATTTGGCAAAAGGAAGTCCAATATTTCTATTCAAAATGCATTAATTTCTCTTTATCAAAAAGAAACTATGGAATGTCCAACATTCAAGTTTATGTTTAAAACTACGCGAGTATAGAGAAATGACTTGTACCTCCAATTGTGCAGTTGTGAAGGACTTGAAATATGCTACCTCTCATGAATGGGTGAAAGTAGAAGGAGACAAGGCAACTGTTGGCATAACTGATCATGCTCAAGATCATTTAGGTGATGTTGTCTATGTTGAATTACCAGAAGTTGGAACCTCTGTTAAACAGGATGGCAGCTTCGGTGCGGTAGAAAGCGTTAAAGCTACCAGTGATATTAACTCTCCTGTGTCTGGGAAAATCATTGAGGTCAATGAAGAGCTCAGCAGTTCCCCTGGTCTGGTAGGTTCCTTGATCTAATACACTTGCTCTTAATAATGACCTGACAATTGCCCTGGAAAGATTTAAGCTTATTTTCTTTCTTCTTTCCGCTTTTGGATACTTTAAGATGGCTTCTATTGACTTGAGTCTTAGTTTCATTACTTGACATGTACCCCCCCCCCAAATTCCAAATTTGCTAAGAAAGATCTGGTTAATGTAAATGTTCAAGTGTAAGGGATAAGGTTTGTTGCACATTTTACAGATACTAACTCTTTTTTTTTGTAAAAGATACCAACTCTTCTTGTGTCATGAAAATGTTGTCATTGGAGGTTGCATATGGCATTTTCTAAATTATTTGGATTATACAGCCTTCTTTGACTGTATTCAGGAGACAACCTGCCTATACCTTTGGATGTTTTCTTAACAATTACTGTTTCTTTTACTATCCTCCCCTTTGCGTCCATTGAAAACTTTCTAGTCATTTTGTAACTTGATGATTGTAAAATACACACATACACAGCAACATTCTCGATCACTTATGTAATTGTCCTTTGTTCCTTATTCTTTGTCCTTCCCTGCATCTTATGAAAGTTTAGGAAAAGTTCCTTTATAGTACTTATTTAGTTTTCTTTGTTTTCTTTGTATGTGAGTATATGAGTGGTTTTTTGTCATCAAATGAAAGCTTAAAAGAATAACCATCACTTAGTTTTCTTTCTCCCAAGCAGGGTTATGAGTTGTTTTTGATCCATTTGTGTAAATGTAGGTGAACTCAAGTCCATATGAAAATGGATGGATAATTAAGGTGGAGGTTAGTGGCAGTGGTGAACTGAAAAACTTGATGGACTCTGAGCAGTACTCCAAGTTCTGTGAAGAAGAAGATTCAAAGCACTGAGAGTTCATAGAGATGTTTGAAGAAGTCAGCAGCTGGGAGAGAGAACTCTGCATCAGATCAGTTGGTTCTCTGGGTTGGCCAGAGAGACTGCACATTTCTTCCTTCCTTTTTCTTTTTCTTCTGTTTTCTGGATTTTCTTTCCTTTGTAAGACTCACTAAATTGGTGGAAATTTTCCCATTCAGATGAATGAATAATTTGATCTCTTTGAAGCTTCAACTAATGGTGTAATGATGAATTTTCTAGTAAAAATATATATACATATTTACGAGGGAAAAATTATAAAA

mRNA sequence

TGGGTTTGTTGTTGACACATTGACCCACCACCTTGCAGCTTCGACGTGGGATTGCTTTCGCAACCTCTATTTTTCTTCATTTCTCTTCCTTGTTTCAATTATTTTCAGATTAATGTTTATGATCTCACATCCCAAAAATAAAAAATGAAATGAAAATTGACCCAAAATACGTAAAATGCAAAGTATAAAACCCAAAAAGAAAAAAGAAATATATATATTATATATATGGTATACGAGCATCCCCACCAACCCTTCCATGAAACTGTTCTCTTAGAGCCGACGGCAAGCTAAAGCATTAGCAGCAAAACAAGCTTCTGTTGAGAAAAGGGTTTTTACAGAGCGCTCTAAGTTTCATCAATGGCGTCCAGGTTGGTTTGGGCTTCAAGAGCTGCTTCTTTTCTCAGGATCTCAATTCCCCTTAGAGGGTTTGCTTCTGTTGTGAAGGACTTGAAATATGCTACCTCTCATGAATGGGTGAAAGTAGAAGGAGACAAGGCAACTGTTGGCATAACTGATCATGCTCAAGATCATTTAGGTGATGTTGTCTATGTTGAATTACCAGAAGTTGGAACCTCTGTTAAACAGGATGGCAGCTTCGGTGCGGTAGAAAGCGTTAAAGCTACCAGTGATATTAACTCTCCTGTGTCTGGGAAAATCATTGAGGTCAATGAAGAGCTCAGCAGTTCCCCTGGTCTGGTGAACTCAAGTCCATATGAAAATGGATGGATAATTAAGGTGGAGGTTAGTGGCAGTGGTGAACTGAAAAACTTGATGGACTCTGAGCAGTACTCCAAGTTCTGTGAAGAAGAAGATTCAAAGCACTGAGAGTTCATAGAGATGTTTGAAGAAGTCAGCAGCTGGGAGAGAGAACTCTGCATCAGATCAGTTGGTTCTCTGGGTTGGCCAGAGAGACTGCACATTTCTTCCTTCCTTTTTCTTTTTCTTCTGTTTTCTGGATTTTCTTTCCTTTGTAAGACTCACTAAATTGGTGGAAATTTTCCCATTCAGATGAATGAATAATTTGATCTCTTTGAAGCTTCAACTAATGGTGTAATGATGAATTTTCTAGTAAAAATATATATACATATTTACGAGGGAAAAATTATAAAA

Coding sequence (CDS)

ATGGCGTCCAGGTTGGTTTGGGCTTCAAGAGCTGCTTCTTTTCTCAGGATCTCAATTCCCCTTAGAGGGTTTGCTTCTGTTGTGAAGGACTTGAAATATGCTACCTCTCATGAATGGGTGAAAGTAGAAGGAGACAAGGCAACTGTTGGCATAACTGATCATGCTCAAGATCATTTAGGTGATGTTGTCTATGTTGAATTACCAGAAGTTGGAACCTCTGTTAAACAGGATGGCAGCTTCGGTGCGGTAGAAAGCGTTAAAGCTACCAGTGATATTAACTCTCCTGTGTCTGGGAAAATCATTGAGGTCAATGAAGAGCTCAGCAGTTCCCCTGGTCTGGTGAACTCAAGTCCATATGAAAATGGATGGATAATTAAGGTGGAGGTTAGTGGCAGTGGTGAACTGAAAAACTTGATGGACTCTGAGCAGTACTCCAAGTTCTGTGAAGAAGAAGATTCAAAGCACTGA

Protein sequence

MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH

Homology

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BLAST of Lsi02G010550 vs. Swiss-Prot
Match: GCSH2_ARATH (Glycine cleavage system H protein 2, mitochondrial OS=Arabidopsis thaliana GN=GDH2 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 4.2e-64
Identity = 123/156 (78.85%), Postives = 138/156 (88.46%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVV-KDLKYATSHEWVKVEGDKATVGITDHAQDHL 60
           MA RL WASR AS LRIS+  RGF+SVV KDLKYA SHEWVK++G+KAT GITDHAQDHL
Sbjct: 1   MACRLFWASRVASHLRISVAQRGFSSVVLKDLKYADSHEWVKIDGNKATFGITDHAQDHL 60

Query: 61  GDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPY 120
           GDVVYVELP+VG SV Q  SFGAVESVKATSDINSPVSGK++EVNEEL+ SPGLVNSSPY
Sbjct: 61  GDVVYVELPDVGHSVSQGKSFGAVESVKATSDINSPVSGKVVEVNEELTESPGLVNSSPY 120

Query: 121 ENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           E GWIIKVE+S +GE + LMDS++YSKFCEEED+KH
Sbjct: 121 EQGWIIKVELSDAGEAEKLMDSDKYSKFCEEEDAKH 156

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BLAST of Lsi02G010550 vs. Swiss-Prot
Match: GCSH_FLAPR (Glycine cleavage system H protein, mitochondrial OS=Flaveria pringlei GN=GDCSH PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 3.8e-49
Identity = 99/158 (62.66%), Postives = 124/158 (78.48%), Query Frame = 1


Query: 6   VWASRAASFLRISI-------PL-RGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQD 65
           +WAS  A+ LR+S        PL R F+SV+  LKYA SHEWVK EG  AT+GITDHAQD
Sbjct: 5   IWASSTANALRLSSATRPHFSPLSRCFSSVLDGLKYANSHEWVKHEGSVATIGITDHAQD 64

Query: 66  HLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSS 125
           HLG+VV+V+LPE G SV +   FGAVESVKATSD+NSP+SG+I+EVN +LS +PGL+NSS
Sbjct: 65  HLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSETPGLINSS 124

Query: 126 PYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           PYE+GW+IKV+ S   EL +LM +++Y+KFCEEEDS H
Sbjct: 125 PYEDGWMIKVKPSNPSELDSLMGAKEYTKFCEEEDSAH 162

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BLAST of Lsi02G010550 vs. Swiss-Prot
Match: GCSH_FLAAN (Glycine cleavage system H protein, mitochondrial OS=Flaveria anomala GN=GDCSH PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-48
Identity = 101/163 (61.96%), Postives = 127/163 (77.91%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISI-------PL-RGFASVVKDLKYATSHEWVKVEGDKATVGIT 60
           MA R+ WAS  A+ LR+S        PL R F+SV+  LKYA SHEWVK EG  AT+GIT
Sbjct: 1   MALRM-WASSTANALRLSSATRPHFSPLSRCFSSVLDGLKYANSHEWVKHEGSVATIGIT 60

Query: 61  DHAQDHLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPG 120
           DHAQDHLG+VV+V+LPE G SV +   FGAVESVKATSD+NSP+SG+I+EVN +LS +PG
Sbjct: 61  DHAQDHLGEVVFVDLPEAGGSVTKATGFGAVESVKATSDVNSPISGEIVEVNSKLSETPG 120

Query: 121 LVNSSPYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           L+NSSPYE+GW+IKV+ S   EL +LM +++Y+KFCEEED+ H
Sbjct: 121 LINSSPYEDGWMIKVKPSNPSELDSLMGAKEYTKFCEEEDAAH 162

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BLAST of Lsi02G010550 vs. Swiss-Prot
Match: GCSH3_ARATH (Glycine cleavage system H protein 3, mitochondrial OS=Arabidopsis thaliana GN=GDH3 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 4.7e-47
Identity = 99/167 (59.28%), Postives = 128/167 (76.65%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRIS--------IPL----RGFASVVKDLKYATSHEWVKVEGDKAT 60
           MA R+ WAS  A+ L++S        +P     R F+SV++ LKYA SHEWVK EG  AT
Sbjct: 1   MALRM-WASSTANALKLSSSASKSHLLPAFSISRCFSSVLEGLKYANSHEWVKHEGSVAT 60

Query: 61  VGITDHAQDHLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELS 120
           +GITDHAQDHLG+VV+VELPE  +SV ++ SFGAVESVKATS+I SP+SG++IEVN +L+
Sbjct: 61  IGITDHAQDHLGEVVFVELPEANSSVSKEKSFGAVESVKATSEILSPISGEVIEVNTKLT 120

Query: 121 SSPGLVNSSPYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
            SPGL+NSSPYE+GW+IKV+ S   EL+ LM  ++Y+KFCEEED+ H
Sbjct: 121 ESPGLINSSPYEDGWMIKVKPSSPAELEALMGPKEYTKFCEEEDAAH 166

[Close]

BLAST of Lsi02G010550 vs. Swiss-Prot
Match: GCSH1_ARATH (Glycine cleavage system H protein 1, mitochondrial OS=Arabidopsis thaliana GN=GDH1 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 8.0e-47
Identity = 98/166 (59.04%), Postives = 128/166 (77.11%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPL-----------RGFASVVKDLKYATSHEWVKVEGDKATV 60
           MA R+ WAS  A+ L++S  +           R F++V++ LKYA SHEWVK EG  AT+
Sbjct: 1   MALRM-WASSTANALKLSSSVSKSHLSPFSFSRCFSTVLEGLKYANSHEWVKHEGSVATI 60

Query: 61  GITDHAQDHLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSS 120
           GIT HAQDHLG+VV+VELPE  TSV ++ SFGAVESVKATS+I SP+SG+IIEVN++L+ 
Sbjct: 61  GITAHAQDHLGEVVFVELPEDNTSVSKEKSFGAVESVKATSEILSPISGEIIEVNKKLTE 120

Query: 121 SPGLVNSSPYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           SPGL+NSSPYE+GW+IKV+ S   EL++LM  ++Y+KFCEEED+ H
Sbjct: 121 SPGLINSSPYEDGWMIKVKPSSPAELESLMGPKEYTKFCEEEDAAH 165

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BLAST of Lsi02G010550 vs. TrEMBL
Match: A0A0A0M3S2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701910 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 8.8e-77
Identity = 150/155 (96.77%), Postives = 151/155 (97.42%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MASRLVWASRAAS+LRISIP RGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQ HLG
Sbjct: 1   MASRLVWASRAASYLRISIPFRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQGHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE
Sbjct: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           NGWIIKVEVS SGELK LMDSEQYSKFCEEEDSKH
Sbjct: 121 NGWIIKVEVSDSGELKKLMDSEQYSKFCEEEDSKH 155

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BLAST of Lsi02G010550 vs. TrEMBL
Match: V4UCP4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017047mg PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 4.1e-66
Identity = 125/155 (80.65%), Postives = 144/155 (92.90%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MASRL+WASRAAS+LRIS+  RGF+SV+KDLKYA SHEWVKV+G+ AT+GITDHAQDHLG
Sbjct: 1   MASRLLWASRAASYLRISVFHRGFSSVIKDLKYADSHEWVKVDGNSATIGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVG +VKQ  SFGAVESVKATSD+NSPVSGK++EVNEELSSSP LVNSSPY+
Sbjct: 61  DVVYVELPEVGVTVKQGASFGAVESVKATSDVNSPVSGKVVEVNEELSSSPALVNSSPYK 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           +GWIIKVE+  +GELK LMD++QY+KFCEEEDSKH
Sbjct: 121 DGWIIKVEMDSAGELKKLMDADQYTKFCEEEDSKH 155

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BLAST of Lsi02G010550 vs. TrEMBL
Match: D7TUC6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0017g01700 PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 3.5e-65
Identity = 124/155 (80.00%), Postives = 138/155 (89.03%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MA  L+WA+RAAS+LRIS+P R F+SVVKDLKY  SHEW KV+G+ AT+GITDHAQDHLG
Sbjct: 1   MAGGLLWATRAASYLRISVPFRAFSSVVKDLKYVDSHEWAKVDGNLATIGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVG +V Q GSFGAVESVKATSDINSPVSGK++EVNEELSSSPGLVN SPYE
Sbjct: 61  DVVYVELPEVGANVSQGGSFGAVESVKATSDINSPVSGKVVEVNEELSSSPGLVNGSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
            GWIIKVE+S   EL +LMDSEQYSKFCEEED+KH
Sbjct: 121 KGWIIKVEISNPSELNSLMDSEQYSKFCEEEDAKH 155

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BLAST of Lsi02G010550 vs. TrEMBL
Match: A0A151SJN8_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_001152 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 4.5e-65
Identity = 127/155 (81.94%), Postives = 140/155 (90.32%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MA R +WASRAAS+LRIS+  RGF++VVKDLKYA SHEWVKV+G+ ATVGITDHAQDHLG
Sbjct: 1   MACRQLWASRAASYLRISVFHRGFSNVVKDLKYADSHEWVKVDGNSATVGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVG +V Q  SFGAVESVKATSDINSPVSGK++EVNEEL SSP LVNSSPYE
Sbjct: 61  DVVYVELPEVGAAVTQGASFGAVESVKATSDINSPVSGKVVEVNEELHSSPALVNSSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           NGWIIKVE+S +GEL NLMD E+YSKFCEEEDSKH
Sbjct: 121 NGWIIKVEMSDNGELNNLMDPEKYSKFCEEEDSKH 155

[Close]

BLAST of Lsi02G010550 vs. TrEMBL
Match: I3S7G1_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 4.5e-65
Identity = 128/155 (82.58%), Postives = 139/155 (89.68%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MA R +WASRAAS+LRIS+  R F+SVVKDLKYA SHEWVKV+G+ ATVGITDHAQDHLG
Sbjct: 1   MACRQLWASRAASYLRISVFNRAFSSVVKDLKYADSHEWVKVDGNSATVGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVGT V Q G FGAVESVKATSDINSPVSGK++EVNEEL+SSP  VNSSPYE
Sbjct: 61  DVVYVELPEVGTEVTQGGGFGAVESVKATSDINSPVSGKVVEVNEELNSSPAKVNSSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           +GWIIKVE+S S EL NLMDSEQYSKFCEEEDSKH
Sbjct: 121 DGWIIKVELSDSSELSNLMDSEQYSKFCEEEDSKH 155

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BLAST of Lsi02G010550 vs. TAIR10
Match: AT2G35120.1 (AT2G35120.1 Single hybrid motif superfamily protein)

HSP 1 Score: 245.4 bits (625), Expect = 2.4e-65
Identity = 123/156 (78.85%), Postives = 138/156 (88.46%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVV-KDLKYATSHEWVKVEGDKATVGITDHAQDHL 60
           MA RL WASR AS LRIS+  RGF+SVV KDLKYA SHEWVK++G+KAT GITDHAQDHL
Sbjct: 1   MACRLFWASRVASHLRISVAQRGFSSVVLKDLKYADSHEWVKIDGNKATFGITDHAQDHL 60

Query: 61  GDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPY 120
           GDVVYVELP+VG SV Q  SFGAVESVKATSDINSPVSGK++EVNEEL+ SPGLVNSSPY
Sbjct: 61  GDVVYVELPDVGHSVSQGKSFGAVESVKATSDINSPVSGKVVEVNEELTESPGLVNSSPY 120

Query: 121 ENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           E GWIIKVE+S +GE + LMDS++YSKFCEEED+KH
Sbjct: 121 EQGWIIKVELSDAGEAEKLMDSDKYSKFCEEEDAKH 156

[Close]

BLAST of Lsi02G010550 vs. TAIR10
Match: AT1G32470.1 (AT1G32470.1 Single hybrid motif superfamily protein)

HSP 1 Score: 188.7 bits (478), Expect = 2.6e-48
Identity = 99/167 (59.28%), Postives = 128/167 (76.65%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRIS--------IPL----RGFASVVKDLKYATSHEWVKVEGDKAT 60
           MA R+ WAS  A+ L++S        +P     R F+SV++ LKYA SHEWVK EG  AT
Sbjct: 1   MALRM-WASSTANALKLSSSASKSHLLPAFSISRCFSSVLEGLKYANSHEWVKHEGSVAT 60

Query: 61  VGITDHAQDHLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELS 120
           +GITDHAQDHLG+VV+VELPE  +SV ++ SFGAVESVKATS+I SP+SG++IEVN +L+
Sbjct: 61  IGITDHAQDHLGEVVFVELPEANSSVSKEKSFGAVESVKATSEILSPISGEVIEVNTKLT 120

Query: 121 SSPGLVNSSPYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
            SPGL+NSSPYE+GW+IKV+ S   EL+ LM  ++Y+KFCEEED+ H
Sbjct: 121 ESPGLINSSPYEDGWMIKVKPSSPAELEALMGPKEYTKFCEEEDAAH 166

[Close]

BLAST of Lsi02G010550 vs. TAIR10
Match: AT2G35370.1 (AT2G35370.1 glycine decarboxylase complex H)

HSP 1 Score: 188.0 bits (476), Expect = 4.5e-48
Identity = 98/166 (59.04%), Postives = 128/166 (77.11%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPL-----------RGFASVVKDLKYATSHEWVKVEGDKATV 60
           MA R+ WAS  A+ L++S  +           R F++V++ LKYA SHEWVK EG  AT+
Sbjct: 1   MALRM-WASSTANALKLSSSVSKSHLSPFSFSRCFSTVLEGLKYANSHEWVKHEGSVATI 60

Query: 61  GITDHAQDHLGDVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSS 120
           GIT HAQDHLG+VV+VELPE  TSV ++ SFGAVESVKATS+I SP+SG+IIEVN++L+ 
Sbjct: 61  GITAHAQDHLGEVVFVELPEDNTSVSKEKSFGAVESVKATSEILSPISGEIIEVNKKLTE 120

Query: 121 SPGLVNSSPYENGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           SPGL+NSSPYE+GW+IKV+ S   EL++LM  ++Y+KFCEEED+ H
Sbjct: 121 SPGLINSSPYEDGWMIKVKPSSPAELESLMGPKEYTKFCEEEDAAH 165

[Close]

BLAST of Lsi02G010550 vs. NCBI nr
Match: gi|659118255|ref|XP_008459025.1| (PREDICTED: glycine cleavage system H protein 2, mitochondrial [Cucumis melo])

HSP 1 Score: 299.3 bits (765), Expect = 3.9e-78
Identity = 152/155 (98.06%), Postives = 153/155 (98.71%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MASRLVWASRAAS+LRISIP RGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG
Sbjct: 1   MASRLVWASRAASYLRISIPFRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE
Sbjct: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           NGWIIKVEVS SGELKNLMDSEQYSKFCEEEDSKH
Sbjct: 121 NGWIIKVEVSDSGELKNLMDSEQYSKFCEEEDSKH 155

[Close]

BLAST of Lsi02G010550 vs. NCBI nr
Match: gi|449455395|ref|XP_004145438.1| (PREDICTED: glycine cleavage system H protein 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 294.3 bits (752), Expect = 1.3e-76
Identity = 150/155 (96.77%), Postives = 151/155 (97.42%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MASRLVWASRAAS+LRISIP RGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQ HLG
Sbjct: 1   MASRLVWASRAASYLRISIPFRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQGHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE
Sbjct: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           NGWIIKVEVS SGELK LMDSEQYSKFCEEEDSKH
Sbjct: 121 NGWIIKVEVSDSGELKKLMDSEQYSKFCEEEDSKH 155

[Close]

BLAST of Lsi02G010550 vs. NCBI nr
Match: gi|568828311|ref|XP_006468487.1| (PREDICTED: glycine cleavage system H protein 2, mitochondrial [Citrus sinensis])

HSP 1 Score: 263.1 bits (671), Expect = 3.1e-67
Identity = 127/155 (81.94%), Postives = 145/155 (93.55%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MASRL+WASRAAS+LRIS+  RGF+SV+KDLKYA SHEWVKV+G+ AT+GITDHAQDHLG
Sbjct: 1   MASRLLWASRAASYLRISVFHRGFSSVIKDLKYADSHEWVKVDGNSATIGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVG +VKQD SFGAVESVKATSD+NSPVSGK++EVNEELSSSP LVNSSPYE
Sbjct: 61  DVVYVELPEVGVTVKQDASFGAVESVKATSDVNSPVSGKVVEVNEELSSSPALVNSSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           +GWIIKVE+  +GELK LMD++QY+KFCEEEDSKH
Sbjct: 121 DGWIIKVEMDNAGELKKLMDADQYTKFCEEEDSKH 155

[Close]

BLAST of Lsi02G010550 vs. NCBI nr
Match: gi|567912773|ref|XP_006448700.1| (hypothetical protein CICLE_v10017047mg [Citrus clementina])

HSP 1 Score: 258.8 bits (660), Expect = 5.9e-66
Identity = 125/155 (80.65%), Postives = 144/155 (92.90%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MASRL+WASRAAS+LRIS+  RGF+SV+KDLKYA SHEWVKV+G+ AT+GITDHAQDHLG
Sbjct: 1   MASRLLWASRAASYLRISVFHRGFSSVIKDLKYADSHEWVKVDGNSATIGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVG +VKQ  SFGAVESVKATSD+NSPVSGK++EVNEELSSSP LVNSSPY+
Sbjct: 61  DVVYVELPEVGVTVKQGASFGAVESVKATSDVNSPVSGKVVEVNEELSSSPALVNSSPYK 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
           +GWIIKVE+  +GELK LMD++QY+KFCEEEDSKH
Sbjct: 121 DGWIIKVEMDSAGELKKLMDADQYTKFCEEEDSKH 155

[Close]

BLAST of Lsi02G010550 vs. NCBI nr
Match: gi|225464818|ref|XP_002269090.1| (PREDICTED: glycine cleavage system H protein 2, mitochondrial [Vitis vinifera])

HSP 1 Score: 255.8 bits (652), Expect = 5.0e-65
Identity = 124/155 (80.00%), Postives = 138/155 (89.03%), Query Frame = 1


Query: 1   MASRLVWASRAASFLRISIPLRGFASVVKDLKYATSHEWVKVEGDKATVGITDHAQDHLG 60
           MA  L+WA+RAAS+LRIS+P R F+SVVKDLKY  SHEW KV+G+ AT+GITDHAQDHLG
Sbjct: 1   MAGGLLWATRAASYLRISVPFRAFSSVVKDLKYVDSHEWAKVDGNLATIGITDHAQDHLG 60

Query: 61  DVVYVELPEVGTSVKQDGSFGAVESVKATSDINSPVSGKIIEVNEELSSSPGLVNSSPYE 120
           DVVYVELPEVG +V Q GSFGAVESVKATSDINSPVSGK++EVNEELSSSPGLVN SPYE
Sbjct: 61  DVVYVELPEVGANVSQGGSFGAVESVKATSDINSPVSGKVVEVNEELSSSPGLVNGSPYE 120

Query: 121 NGWIIKVEVSGSGELKNLMDSEQYSKFCEEEDSKH 156
            GWIIKVE+S   EL +LMDSEQYSKFCEEED+KH
Sbjct: 121 KGWIIKVEISNPSELNSLMDSEQYSKFCEEEDAKH 155

The following BLAST results are available for this feature:

BLAST of Lsi02G010550 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GCSH2_ARATH	4.2e-64	78.85	Glycine cleavage system H protein 2, mitochondrial OS=Arabidopsis thaliana GN=GD...	[more]
GCSH_FLAPR	3.8e-49	62.66	Glycine cleavage system H protein, mitochondrial OS=Flaveria pringlei GN=GDCSH P...	[more]
GCSH_FLAAN	1.5e-48	61.96	Glycine cleavage system H protein, mitochondrial OS=Flaveria anomala GN=GDCSH PE...	[more]
GCSH3_ARATH	4.7e-47	59.28	Glycine cleavage system H protein 3, mitochondrial OS=Arabidopsis thaliana GN=GD...	[more]
GCSH1_ARATH	8.0e-47	59.04	Glycine cleavage system H protein 1, mitochondrial OS=Arabidopsis thaliana GN=GD...	[more]

BLAST of Lsi02G010550 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0M3S2_CUCSA	8.8e-77	96.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G701910 PE=3 SV=1	[more]
V4UCP4_9ROSI	4.1e-66	80.65	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10017047mg PE=3 SV=1	[more]
D7TUC6_VITVI	3.5e-65	80.00	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0017g01700 PE=3 SV=...	[more]
A0A151SJN8_CAJCA	4.5e-65	81.94	Uncharacterized protein OS=Cajanus cajan GN=KK1_001152 PE=3 SV=1	[more]
I3S7G1_LOTJA	4.5e-65	82.58	Uncharacterized protein OS=Lotus japonicus PE=2 SV=1	[more]

BLAST of Lsi02G010550 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G35120.1	2.4e-65	78.85	Single hybrid motif superfamily protein	[more]
AT1G32470.1	2.6e-48	59.28	Single hybrid motif superfamily protein	[more]
AT2G35370.1	4.5e-48	59.04	glycine decarboxylase complex H	[more]

BLAST of Lsi02G010550 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659118255\|ref\|XP_008459025.1\|	3.9e-78	98.06	PREDICTED: glycine cleavage system H protein 2, mitochondrial [Cucumis melo]	[more]
gi\|449455395\|ref\|XP_004145438.1\|	1.3e-76	96.77	PREDICTED: glycine cleavage system H protein 2, mitochondrial [Cucumis sativus]	[more]
gi\|568828311\|ref\|XP_006468487.1\|	3.1e-67	81.94	PREDICTED: glycine cleavage system H protein 2, mitochondrial [Citrus sinensis]	[more]
gi\|567912773\|ref\|XP_006448700.1\|	5.9e-66	80.65	hypothetical protein CICLE_v10017047mg [Citrus clementina]	[more]
gi\|225464818\|ref\|XP_002269090.1\|	5.0e-65	80.00	PREDICTED: glycine cleavage system H protein 2, mitochondrial [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0019464	glycine decarboxylation via glycine cleavage system

Vocabulary: **Cellular Component**
Term	Definition
GO:0005960	glycine cleavage complex

Vocabulary: **INTERPRO**
Term	Definition
IPR017453	GCV_H_sub
IPR011053	Single_hybrid_motif
IPR003016	2-oxoA_DH_lipoyl-BS
IPR002930	GCV_H
IPR000089	Biotin_lipoyl

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019464	glycine decarboxylation via glycine cleavage system
biological_process	GO:0009750	response to fructose
biological_process	GO:0009749	response to glucose
biological_process	GO:0009744	response to sucrose
cellular_component	GO:0005960	glycine cleavage complex
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G010550.1	Lsi02G010550.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000089	Biotin/lipoyl attachment	PROFILE	PS50968	BIOTINYL_LIPOYL	coord: 46..128 score: 30
IPR002930	Glycine cleavage system H-protein	HAMAP	MF_00272	GcvH	coord: 28..149 score: 29
IPR002930	Glycine cleavage system H-protein	PANTHER	PTHR11715	GLYCINE CLEAVAGE SYSTEM H PROTEIN	coord: 1..155 score: 4.3E
IPR002930	Glycine cleavage system H-protein	PFAM	PF01597	GCV_H	coord: 31..150 score: 2.3
IPR003016	2-oxo acid dehydrogenase, lipoyl-binding site	PROSITE	PS00189	LIPOYL	coord: 71..100 scor
IPR011053	Single hybrid motif	unknown	SSF51230	Single hybrid motif	coord: 27..150 score: 4.93
IPR017453	Glycine cleavage system H-protein, subgroup	TIGRFAMs	TIGR00527	TIGR00527	coord: 28..152 score: 1.2
None	No IPR available	GENE3D	G3DSA:2.40.50.100		coord: 29..151 score: 7.4
None	No IPR available	PANTHER	PTHR11715:SF9	SUBFAMILY NOT NAMED	coord: 1..155 score: 4.3E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi02G010550	Csa1G701910	Cucumber (Chinese Long) v2	culsiB033
Lsi02G010550	CSPI01G34390	Wild cucumber (PI 183967)	cpilsiB034
Lsi02G010550	Cucsa.332790	Cucumber (Gy14) v1	cgylsiB539
Lsi02G010550	CmaCh13G004250	Cucurbita maxima (Rimu)	cmalsiB207
Lsi02G010550	CmoCh13G004560	Cucurbita moschata (Rifu)	cmolsiB197
Lsi02G010550	Cla004609	Watermelon (97103) v1	lsiwmB206
Lsi02G010550	MELO3C021742	Melon (DHL92) v3.5.1	lsimeB180
Lsi02G010550	ClCG01G009540	Watermelon (Charleston Gray)	lsiwcgB169
Lsi02G010550	MELO3C021742.2	Melon (DHL92) v3.6.1	lsimedB190
Lsi02G010550	Cla97C01G009760	Watermelon (97103) v2	lsiwmbB153
Lsi02G010550	Bhi08G000720	Wax gourd	lsiwgoB255
Lsi02G010550	CsGy1G032860	Cucumber (Gy14) v2	cgyblsiB030
Lsi02G010550	CsaV3_1G046250	Cucumber (Chinese Long) v3	cuclsiB034

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi02G010550	Cucurbita maxima (Rimu)	cmalsiB369
Lsi02G010550	Cucurbita moschata (Rifu)	cmolsiB356
Lsi02G010550	Cucurbita pepo (Zucchini)	cpelsiB026
Lsi02G010550	Cucurbita pepo (Zucchini)	cpelsiB417
Lsi02G010550	Silver-seed gourd	carlsiB104