Cucsa.393870 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.393870
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionDNA-directed RNA polymerase subunit
Locationscaffold04100 : 23106 .. 26913 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCCAACCCTATGTAATGGCGTATGGGAAAATAATGGAAAACCAAATTCTCTTGAAGGAAAAAAGAAAAGCAAAACCCTATTTTCTCACCTTCTAGCCCCAGGCCTTCGTGATTTTTCAGACTTACCTTTCTTGCTGGTTCGTCACCCGCCTCCGCCGCTCGTTACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAAAACAGTAAACCATATTCATCGTTTCTCTTCCATCAATGATCCACCGACCTTGGTGGCGATCATTTTGAATGAAATCGAAATTCTGATATCCGACTGTTGGCTTCCGTCCGCTTCCAATTTCCGCGCGGTTCGATGAATATCCACACATTCTAGAGGTCGGTTCGGTCCAGATTTCTTTTTCGAACCGGACCGACTTTGCAGTGCTGCTCCTTGGCCTCGAGGGTTCTAGCTTCCAAAACTCAAAACCCAGGATGATACCATCATGTTTTCACTCTTCTGCTTTAGCAAGTTTTCTCTAAGGTCGATCATTGATCCATATCTCCGACCATCTCTGCTTTCCTTGGCTAACTTTTCGACAAGAACTCTGTAAAAGCTCTTTCTCGAGCTTCATTCATTTTAGCAGAAATATTGAACTATAGAGGATATCTGGGCTAGTTCGAGGTTATCAAACCGATCAAATTGAGCTTTCTACTTGTTATCTTTCGGATATCTGAATGACCCATCTTAAGTTGTTATTTTGATACATTATGTTCGTTTTAGTCTATTGTGGGTATGAGTTTCTTCATGCTTTTCCATTGAGGTTTTAGTTTATTGACTGGTTCTAGGTTTTTTGAAATGGAAACAAATTTTGAGAATTGACTGGTTCTAGGTGAAGCTCCAATTGTTTACCCAAATCCCCTTTTTGCTATTACGATTCTACCACCCTTTTTAGTCTCAAATTTCCTGTCTTTTAGAGTGTTAGCAATTTTCTACTCTTACTTGCTGTACTTGAACTTCTTATGGTTATTCTAACTCTCTCTTTTTTTTCTTTTTTTTACTATGGACATTGTTAGGTAGGAAGCTATCGGGGAACCGGCAAGCACCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGCGGAACCATGTTGTCGTTCTGCTCAACGAAATATGTTGAGTGTCCTTCGTGCAAGTCTAGGCGAAGTGCAAAAGGTGGCCATAAACATTGGACTTAAGCTTATTGCATTGTAGTGATTTACTTTTATTCTTTTGTTTTGGATAATTTAAACATTCCAACACTCATTACGTATATGAATAAGTTTTTAGTCTGGTATGTCTTGCTACACGTAAACTCTGTTTTTATCTGATTAAATTGTGGTTTAGTTTATTTCCCCTTCATATTTATACGAGTAAAATGTGAACTATCGCCTATTTTTCCATTTCCTATCTTGAGTTTCTGTATTTCTTTTTAATACTTTTACTAGAATAGACTGTGTCTGGGTTGTGCAGGTTGGTTGTTCTTAATCTATCAACTTCAATTTTTTTAAAAAAACTTATTGTAGATAGGAGAGTAAGAGTTTCTATATGGGAATTTGCAGCATCTTCTGCTTTGAGTGTTCAGCTTTGAAAACATGGTTTCTTACGCAACATCAGTGTTCATCTGTTTAACAGGAGTAGTAGTTTTTTGTTTGAGAAGGTTATTGCTGCGCTGATTGCTTATGCATTTTGGTTTGAATCAAAGCATAGATAAGTTGACTATCATTATTTTGATGAAAAGCTAGCTTCCATTAGGATGGATGATTGTAAAAAAAAAAAAAAAAACTATCTCAAATAGGACACAAAGAATCTTCCACTTAGAGTAAGCAGTTGAAGCCATCTGCGGATAAAAAAAGGGTGAAAGATAATCCCTTGATGTGGCCAAACCTTTCATTTAGTTTTTCTATTCAGAAAAGAACCAAGAAGTCCACAGACGAGAGTCATTCTTTAATTCATATTATCATTCTCTGCCAACACATTACCCCTGAGGATTGAATATAATTTGGAACGATACAGCAAAAGCCAGAGGAATTTTTGAGATGATTTTAGAGAGGATAGTTTTGAACCTATTTTCAAACATTTATCAAGAATTTTGTAAGAGCCCATGGCTAGGGAGGCTTGTGGGTCGGTTGCCTTTCACTGGTCATCCGTAGCCATAAAGGCAGTATAAATCTTGGAAAATTCACACCAAGAATTCCTTTCTTAAAGAAGCCTAGATGTTGACATGGTATCATGCTAGAGAGTATCCCACAGGAGTAGAAATAGCCGTTGGAACTTTATGCTAAATAAAAGCCTACAGGAGTAGAGAACCGGCTGTAAAAATAGAATTCCAACAAACCATTGAAGATTATGTCGTCCTCTCTGCTTAGTACAGTAGACTGCTGGGGCTTCGACTATTGGAGCCTTTGATTTCTTCTGAAACCTATTTTACTACTATGGATGACTTTTATAACTTTTCCATTAAAAATGCTAAAGGGAAAAAGAGATACCCTGAGAAGGTTAAATTCTCTGTTTGGGACGTTGATCTTTCAGATGTCCTCCCTCTCTCTGACTCAGTTATTTAACTAATCAGTTAGCTTCCTTTTACAAATAAGAACTGAACCTGTATCGTAGGTATAGAAATTAACATAAATACTGCTTAATAAGACTTCTACTCATCTCCTCATACACTATCGTTCTTGGTAGTATTGCGCCCAAGATGTGTGACAACTCATTTAAAATTTTCAGGCTGGTTACTCGTCTTATACAAGGGCCCAAGTGCCATCATGATTTCCCTTAATGGTATTCAATTATACATCTTGAAATTGGTGTTCTGAAGGGCGTATACCAAATTATTTTAGAAGTTGATGCTATGTTTTCGTGATTGGAGATATTGAAGTTGAGAAAATGCAATTGAGAAACTACTGTTGTAATATTTTAAGCACTCTTGTATTTGACATCTCTAACCGAATTTTTGTGGCTTATTTCAGAGATCGTCGGTAGAGAAATAAGTTACACAGTAACTGCTGAGGTATGTCTTTTAAATCATACACATTAATATGTTATCCCCATGCTTTCTAGGTCTAAGGCACAGTATCCTCTCTGCAACACGACCTTAGCAATGCTTTTAAACTTGAGCATTCTCTTATAATTTCTGCTACTTTTATGATGCTTTCTAGGATTGTTTCTGGATGAATTATTTGTAGATTGTGAGAAGGGCTTAAACAACTGTATAATGTTCTCTAGGTCCAGGCAAATCAATAAACCAGACCCTGGAAATTCTTATTCGGTCAACATCTGGAACTGTGAAAATTTTTAGTATTAGCAAACGTTCATAATGATCTATTATTGATACGATGCTCCCTTATGAGTTGTCTGATATCAAAGAAATGTTTCATTTTGAAATCCTCGTGTGGCAATTTGCAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGTTAGCAAAGGTAGTAGGAGAAAATAAGGTGTATGTATGGTATTCAATTTTGCTTGAGATACAGTCCACTAATATGAAGCTTTGTGATGTTTGGCAGGAAAGGAGAAGATGTGAAAAATGTGGAAATGATGAAGCTTGGTTTGAGAGCAGACAGGTACACTTTACAAACATTAACAAAATTAGAATTGTGGGTTATTTAGGGTTTATTTCTGTCTTTTCTTCCATTAGAAAGAGTAATAAGTTTGAGTGTGGTTTGAATAAAAATGCAGATGAGATCGGCTGATGAAGGCCAGACCACATTTTATACCTGCACTAAATGTCATCATCAGACTAGAGAGAATTGA

mRNA sequence

GCCCAACCCTATGTAATGGCGTATGGGAAAATAATGGAAAACCAAATTCTCTTGAAGGAAAAAAGAAAAGCAAAACCCTATTTTCTCACCTTCTAGCCCCAGGCCTTCGTGATTTTTCAGACTTACCTTTCTTGCTGGTTCGTCACCCGCCTCCGCCGCTCGTTACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAGGAAGCTATCGGGGAACCGGCAAGCACCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGCGGAACCATGTTGTCGTTCTGCTCAACGAAATATGTTGAGTGTCCTTCGTGCAAGTCTAGGCGAAGTGCAAAAGAGATCGTCGGTAGAGAAATAAGTTACACAGTAACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGTTAGCAAAGGAAAGGAGAAGATGTGAAAAATGTGGAAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGCCAGACCACATTTTATACCTGCACTAAATGTCATCATCAGACTAGAGAGAATTGA

Coding sequence (CDS)

GCCCAACCCTATGTAATGGCGTATGGGAAAATAATGGAAAACCAAATTCTCTTGAAGGAAAAAAGAAAAGCAAAACCCTATTTTCTCACCTTCTAGCCCCAGGCCTTCGTGATTTTTCAGACTTACCTTTCTTGCTGGTTCGTCACCCGCCTCCGCCGCTCGTTACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAGGAAGCTATCGGGGAACCGGCAAGCACCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGCGGAACCATGTTGTCGTTCTGCTCAACGAAATATGTTGAGTGTCCTTCGTGCAAGTCTAGGCGAAGTGCAAAAGAGATCGTCGGTAGAGAAATAAGTTACACAGTAACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGTTAGCAAAGGAAAGGAGAAGATGTGAAAAATGTGGAAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGCCAGACCACATTTTATACCTGCACTAAATGTCATCATCAGACTAGAGAGAATTGA

Protein sequence

PTLCNGVWENNGKPNSLEGKKKSKTLFSHLLAPGLRDFSDLPFLLVRHPPPPLVTGSRPDKSSLGRKLSGNRQAPLMAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN*
Homology
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BLAST of Cucsa.393870 vs. Swiss-Prot
Match: RPA12_MACMU (DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta GN=ZNRD1 PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.6e-12
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 1 

Query: 84  DFLFCNLCGTMLSFCSTK-YVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 143
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCTRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 144 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]
BLAST of Cucsa.393870 vs. Swiss-Prot
Match: RPA12_BOVIN (DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus GN=ZNRD1 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 37/110 (33.64%), Postives = 60/110 (54.55%), Query Frame = 1 

Query: 84  DFLFCNLCGTMLSFCSTK-YVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 143
           D  FC  CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 13  DLDFCPDCGSVLPLPGVQDAVACTRCGFSINVRDFEGKVVKTSVVFNKLGTAMPLSMEEG 72

Query: 144 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 73  PEFQGPVVDRRCSRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 122

[Close]
BLAST of Cucsa.393870 vs. Swiss-Prot
Match: RPA12_PANTR (DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes GN=ZNRD1 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.7e-12
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 1 

Query: 84  DFLFCNLCGTMLSFCSTK-YVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 143
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 144 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]
BLAST of Cucsa.393870 vs. Swiss-Prot
Match: RPA12_HUMAN (DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens GN=ZNRD1 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.7e-12
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 1 

Query: 84  DFLFCNLCGTMLSFCSTK-YVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 143
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 144 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

[Close]
BLAST of Cucsa.393870 vs. Swiss-Prot
Match: RPA12_SCHPO (DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=rpa12 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.1e-12
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 1 

Query: 86  LFCNLCGTMLSFCSTKYVECPSCKS----RRSAKEIVGREISYTVTAEEIRKQLGISLID 145
           +FC+ CG +L   + ++  C  C+S     + A  +V  + S +     ++ +  I  ++
Sbjct: 8   IFCSECGNLLESTTAQWTTCDQCQSVYPSEQFANLVVETKSSASAFPSALKLKHSIVQVE 67

Query: 146 EEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
            +K + A    +C KCGND   F + Q+RSADEG T FY C +C ++   N
Sbjct: 68  SQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFSTN 118

[Close]
BLAST of Cucsa.393870 vs. TrEMBL
Match: A0A0A0LKC4_CUCSA (DNA-directed RNA polymerase subunit OS=Cucumis sativus GN=Csa_2G345940 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-62
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Cucsa.393870 vs. TrEMBL
Match: B3FNK6_CUCSA (DNA-directed RNA polymerase subunit OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.3e-56
Identity = 109/116 (93.97%), Postives = 111/116 (95.69%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYSRGHDFLFCNL GTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEI+KQLG
Sbjct: 1   MAYSRGHDFLFCNLGGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIKKQLG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           ISLIDE  MQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTC+KC  QTREN
Sbjct: 61  ISLIDENNMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCSKCLPQTREN 116

[Close]
BLAST of Cucsa.393870 vs. TrEMBL
Match: G7JQD3_MEDTR (DNA-directed RNA polymerase subunit OS=Medicago truncatula GN=MTR_3g102420 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.7e-37
Identity = 74/114 (64.91%), Postives = 95/114 (83.33%), Query Frame = 1 

Query: 79  YSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGIS 138
           YSRG DFLFC+LCGTML+  ST Y  CP CK++ + K+I G+EISYT++AE+IR++LGI 
Sbjct: 2   YSRGRDFLFCHLCGTMLTVPSTDYALCPLCKTKCNIKDIKGKEISYTISAEDIRRELGID 61

Query: 139 LIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           LI+E+K+QL+K  + CEKCG+ EA F +RQMRSADEGQTTFYTCT+C HQ +EN
Sbjct: 62  LIEEQKVQLSKVNKTCEKCGHGEAAFYTRQMRSADEGQTTFYTCTRCGHQFQEN 115

[Close]
BLAST of Cucsa.393870 vs. TrEMBL
Match: A0A151TUW7_CAJCA (DNA-directed RNA polymerase subunit OS=Cajanus cajan GN=KK1_010091 PE=3 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.9e-36
Identity = 74/116 (63.79%), Postives = 98/116 (84.48%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MA SR  DFLFCNLCGT+L+  ST+Y +CP CK+RR+ + I  REISYT+ AE+IR++LG
Sbjct: 1   MASSR-LDFLFCNLCGTLLTVPSTEYAQCPLCKTRRNIQHIRDREISYTIYAEDIRRELG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           + +I+E+K+QL+K  ++CEKCG+DEA F +RQMRSADEGQTTFYTCT+C HQ++EN
Sbjct: 61  MEVIEEQKVQLSKVNKKCEKCGHDEANFYTRQMRSADEGQTTFYTCTRCGHQSQEN 115

[Close]
BLAST of Cucsa.393870 vs. TrEMBL
Match: V7CX97_PHAVU (DNA-directed RNA polymerase subunit OS=Phaseolus vulgaris GN=PHAVU_001G175600g PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.7e-34
Identity = 70/116 (60.34%), Postives = 95/116 (81.90%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MA SR  DFLFC+LCGTML+  +++Y  CP CK+ R  ++I  +EI YT+ AE+IR++LG
Sbjct: 1   MASSR-LDFLFCHLCGTMLTVPNSEYAVCPLCKTLRDIQDIRDKEIGYTIYAEDIRRELG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           I +I+E+K+QL+K  ++CEKCG+DEA F +RQMRSADEGQTTFYTCT+C HQ++EN
Sbjct: 61  IEIIEEQKVQLSKVNKKCEKCGHDEANFYTRQMRSADEGQTTFYTCTRCGHQSQEN 115

[Close]
BLAST of Cucsa.393870 vs. TAIR10
Match: AT3G25940.1 (AT3G25940.1 TFIIB zinc-binding protein)

HSP 1 Score: 138.7 bits (348), Expect = 3.9e-33
Identity = 66/118 (55.93%), Postives = 87/118 (73.73%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           M  SR  +FLFCNLCGTML   STKY ECP CK+ R+AK+I+ +EI+YTV+AE+IR++LG
Sbjct: 1   MEKSRESEFLFCNLCGTMLVLKSTKYAECPHCKTTRNAKDIIDKEIAYTVSAEDIRRELG 60

Query: 137 ISLIDEE---KMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 192
           ISL  E+   + +L K ++ CEKC + E  + +RQ RSADEGQTT+YTC  C H+  E
Sbjct: 61  ISLFGEKTQAEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYYTCPNCAHRFTE 118

[Close]
BLAST of Cucsa.393870 vs. TAIR10
Match: AT1G20065.2 (AT1G20065.2 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 70.1 bits (170), Expect = 1.7e-12
Identity = 31/53 (58.49%), Postives = 38/53 (71.70%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAE 130
           M  SR  D LFCNLCGTML   STKY ECP C++ R+ KEI+ + ++YTVT E
Sbjct: 1   MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGKEIIDKNLAYTVTTE 53

[Close]
BLAST of Cucsa.393870 vs. TAIR10
Match: AT1G01210.1 (AT1G01210.1 DNA-directed RNA polymerase, subunit M, archaeal)

HSP 1 Score: 58.2 bits (139), Expect = 6.7e-09
Identity = 35/114 (30.70%), Postives = 55/114 (48.25%), Query Frame = 1 

Query: 87  FCNLCGTMLS---------FCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGI 146
           FC  CG +L          FCST    CP     +   EI  +++        ++K +  
Sbjct: 3   FCPTCGNLLRYEGGGNSRFFCST----CPYVAYIQRQVEIKKKQLL-------VKKSIEA 62

Query: 147 SLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 192
            +  ++    A+    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  VVTKDDIPTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]
BLAST of Cucsa.393870 vs. TAIR10
Match: AT4G07950.1 (AT4G07950.1 DNA-directed RNA polymerase, subunit M, archaeal)

HSP 1 Score: 58.2 bits (139), Expect = 6.7e-09
Identity = 35/114 (30.70%), Postives = 55/114 (48.25%), Query Frame = 1 

Query: 87  FCNLCGTMLS---------FCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLGI 146
           FC  CG +L          FCST    CP   +     EI  +++        ++K +  
Sbjct: 3   FCPTCGNLLRYEGGGSSRFFCST----CPYVANIERRVEIKKKQLL-------VKKSIEP 62

Query: 147 SLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 192
            +  ++    A+    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  VVTKDDIPTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]
BLAST of Cucsa.393870 vs. NCBI nr
Match: gi|449450514|ref|XP_004143007.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus])

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-62
Identity = 116/116 (100.00%), Postives = 116/116 (100.00%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Cucsa.393870 vs. NCBI nr
Match: gi|659089115|ref|XP_008445334.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12-like [Cucumis melo])

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-59
Identity = 112/116 (96.55%), Postives = 113/116 (97.41%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYSRGHDFLFCNLCGTMLSFCSTKYVECP CKS+RSAKEI GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSKRSAKEINGREISYTVTAEEIRKQLG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           ISLI EEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIGEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]
BLAST of Cucsa.393870 vs. NCBI nr
Match: gi|821595252|ref|NP_001295812.1| (DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus])

HSP 1 Score: 227.6 bits (579), Expect = 1.8e-56
Identity = 109/116 (93.97%), Postives = 111/116 (95.69%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYSRGHDFLFCNL GTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEI+KQLG
Sbjct: 1   MAYSRGHDFLFCNLGGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIKKQLG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           ISLIDE  MQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTC+KC  QTREN
Sbjct: 61  ISLIDENNMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCSKCLPQTREN 116

[Close]
BLAST of Cucsa.393870 vs. NCBI nr
Match: gi|1009135164|ref|XP_015884839.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 182.6 bits (462), Expect = 6.7e-43
Identity = 82/116 (70.69%), Postives = 102/116 (87.93%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYS G DFLFCNLCGT+L+F STK+ +CP CKS++  K+I+GREISYTVTAE+IR++LG
Sbjct: 1   MAYSHGRDFLFCNLCGTVLAFGSTKHAQCPLCKSKQKLKDIIGREISYTVTAEDIRRELG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           I LI EEK++L+K +RRCEKCG+DEA + +RQMRSADEGQTTFYTCTKC HQ ++N
Sbjct: 61  IVLIGEEKVELSKIKRRCEKCGHDEASYYTRQMRSADEGQTTFYTCTKCQHQFQDN 116

[Close]
BLAST of Cucsa.393870 vs. NCBI nr
Match: gi|1009135166|ref|XP_015884840.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X2 [Ziziphus jujuba])

HSP 1 Score: 178.7 bits (452), Expect = 9.6e-42
Identity = 81/116 (69.83%), Postives = 101/116 (87.07%), Query Frame = 1 

Query: 77  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 136
           MAYS G DFLFCNL GT+L+F STK+ +CP CKS++  K+I+GREISYTVTAE+IR++LG
Sbjct: 1   MAYSHGRDFLFCNLVGTVLAFGSTKHAQCPLCKSKQKLKDIIGREISYTVTAEDIRRELG 60

Query: 137 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 193
           I LI EEK++L+K +RRCEKCG+DEA + +RQMRSADEGQTTFYTCTKC HQ ++N
Sbjct: 61  IVLIGEEKVELSKIKRRCEKCGHDEASYYTRQMRSADEGQTTFYTCTKCQHQFQDN 116

The following BLAST results are available for this feature:
BLAST of Cucsa.393870 vs. Swiss-Prot
Match NameE-valueIdentityDescription
RPA12_MACMU1.6e-1233.64DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta GN=ZNRD1 PE=3 SV=1[more]
RPA12_BOVIN2.1e-1233.64DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus GN=ZNRD1 PE=2 SV=1[more]
RPA12_PANTR2.7e-1233.64DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes GN=ZNRD1 PE=3 SV=... [more]
RPA12_HUMAN2.7e-1233.64DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens GN=ZNRD1 PE=2 SV=1[more]
RPA12_SCHPO6.1e-1230.63DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain... [more]
BLAST of Cucsa.393870 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LKC4_CUCSA2.0e-62100.00DNA-directed RNA polymerase subunit OS=Cucumis sativus GN=Csa_2G345940 PE=3 SV=1[more]
B3FNK6_CUCSA1.3e-5693.97DNA-directed RNA polymerase subunit OS=Cucumis sativus PE=2 SV=1[more]
G7JQD3_MEDTR1.7e-3764.91DNA-directed RNA polymerase subunit OS=Medicago truncatula GN=MTR_3g102420 PE=3 ... [more]
A0A151TUW7_CAJCA1.9e-3663.79DNA-directed RNA polymerase subunit OS=Cajanus cajan GN=KK1_010091 PE=3 SV=1[more]
V7CX97_PHAVU6.7e-3460.34DNA-directed RNA polymerase subunit OS=Phaseolus vulgaris GN=PHAVU_001G175600g P... [more]
BLAST of Cucsa.393870 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G25940.13.9e-3355.93 TFIIB zinc-binding protein[more]
AT1G20065.21.7e-1258.49 FUNCTIONS IN: molecular_function unknown[more]
AT1G01210.16.7e-0930.70 DNA-directed RNA polymerase, subunit M, archaeal[more]
AT4G07950.16.7e-0930.70 DNA-directed RNA polymerase, subunit M, archaeal[more]
BLAST of Cucsa.393870 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449450514|ref|XP_004143007.1|2.9e-62100.00PREDICTED: DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus][more]
gi|659089115|ref|XP_008445334.1|1.3e-5996.55PREDICTED: DNA-directed RNA polymerase I subunit RPA12-like [Cucumis melo][more]
gi|821595252|ref|NP_001295812.1|1.8e-5693.97DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus][more]
gi|1009135164|ref|XP_015884839.1|6.7e-4370.69PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X1 [Ziziphus juju... [more]
gi|1009135166|ref|XP_015884840.1|9.6e-4269.83PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X2 [Ziziphus juju... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001222Znf_TFIIS
Vocabulary: Biological Process
TermDefinition
GO:0006351transcription, DNA-templated
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0008270zinc ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0006206 pyrimidine nucleobase metabolic process
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005730 nucleolus
cellular_component GO:0005575 cellular_component
molecular_function GO:0003899 DNA-directed RNA polymerase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.393870.1Cucsa.393870.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001222Zinc finger, TFIIS-typePFAMPF01096TFIIS_Ccoord: 153..188
score: 1.9
IPR001222Zinc finger, TFIIS-typeSMARTSM00440Cys4_2coord: 152..191
score: 3.7
IPR001222Zinc finger, TFIIS-typePROFILEPS51133ZF_TFIIS_2coord: 150..190
score: 12
NoneNo IPR availableGENE3DG3DSA:2.20.25.10coord: 142..192
score: 4.9
NoneNo IPR availablePANTHERPTHR11239DNA-DIRECTED RNA POLYMERASEcoord: 84..192
score: 1.2
NoneNo IPR availablePANTHERPTHR11239:SF14DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12coord: 84..192
score: 1.2
NoneNo IPR availableunknownSSF57783Zinc beta-ribboncoord: 131..192
score: 9.64