CSPI02G16700 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G16700
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	DNA-directed RNA polymerase subunit
Location	Chr2 : 16026042 .. 16030267 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGATTGTTTATAGAAGAATTGCCAAAATAATAAAAGTAATTATAAGTCTAAAGTATTTATGTGCTACGGTTTCCTATGGTTTCGTTAGGCCCAACCCTATGTAATGGCGTATGGGAAAATAATGGAAAACCAAATTCTCTTGAAGGAAAAAAGAAAAGCAAAACCCTATTTTCTCACCTTCTAGCCCCAGGCCTTCGTGATTTTTCAGACTTACCTTTCTTGCTGGTTCGTCACCCGCCTCCGCCGCTCGTTACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAAAACAGTAAACCATATTCATCGTTTCTCTTCCATCAATGATCCACCGACCTTGGTGGCGATCATTTTGAATGAAATCGAAATTCTGATATCCGACTGTTGGCTTCCGTCCGCTTCCAATTTCCGCGCGGTTCGATGAATATCCACACATTCTAGAGGTCGGTTCGGTCCAGATTTCTTTTTCGAACCGGACCGACTTTGCAGTGCTGCTCCTTGACCTCGAGGGTTCTAGCTTCCAAAACTCAAAACCCAGGATGATACCATCATGTTTTCACTCTTCTGCTTTAGCAAGTTTTCTCTAAGGTCGATCATTGATCCATATCTCCGCCCATATCTGCTTTCCTTGGCTAACTTTTCGACAAGAACTCTGTAAAAGCTCTTTCTCGAGCTTCATTCATTTTAGCAGAAATATTGAACTATAGAGGATATCTGGGCTAGTTCGAGGTTATCAAACCGATCAAATTGAGCTTTCTACTTGTTATCTTTCGGATATCTGAAGACCCATCTTAAGTTGTTATTTTGATATATTATGTTCGTTTTAATCTATTGTGGGTATGAGTTTCTTCATGCTTTTCCATTGAGGTTTTAGTTTATTGACTGGTTCTAGGTTTTTTGAAATGGAAACAAATTTTGAGAATTGACTGGTTCTAGGTGAAGCTCCAATTGTTTACCCAAATTCCCTTTTTGCTATTACGATTCTACCACCCTTTTTAGTCTCAAATTTCCTGTCTTTTAGAGTGTTAGCAATTTTCTACTCTTACTTGCTGTACTTGAACTTCTTATGGTTATTCTAACTCTCTCTTTTTTTTCTTTTTTTTACTATGGACATTGTTAGGTAGGAAGCTATCGGGGAACCGGCAAGCACCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGCGGAACCATGTTGTCGTTCTGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCTAGGCGAAGTGCAAAAGGTGGCCATAAACATTGGACTTAAGCTTATTGCATTGTAGTGATTTACTTTTATTCTTTTGTTTTGGATAATTTAAACATTCCAACACTCATTACGTATATGAATAAGTTTTTAGTCTGGTATGTCTTGCTACACGTAAACTCTGTTTTTATCTGATTAAATTGTGGTTTAGTTTATTTTCCCTTCATATTTATACGAGTAAAATGTGAACTATCGCCTATTTTTCCATTTCCTATCTTGAGATTCTGTATTTCTTTTTAATACTTTTACTAGAATAGACTGTGTCTGGCTTGTGCAGGTTGGTTGTTCTTAATCTATCAACTTCAATTTTTTTAAAAAAACTTATTGTAGATAGGAGAGTAAGAGTTTCTATATGGGAATTTGCAGCATCTTCTGCTTTGAGTGTTCAGCTTTGAAAACATGGTTTCTTACGCAATATCAGTGTTCATCTGTTTAACAGGAGTAGTAGATTTTTGTTTGAGAACGTTATTGCTGCGCTGCTTGCTTATGCATTTTGGTTTGAATCAAAGCATAGATAAGTTGACTATCATTATTTTGATGAAAAGCTAGCTTCCATTAGGATGGATGATTGTTAAAATATCAGTGTTCATCTGTTTAACAGGAGTAGTAGATTTTTGTTTGAGAACGTTATTGCTGCGCTGCTTGCTTATGCATTTTGGTTTGAATCAAAGCATAGATAAGTTGACTATCATTATTTTGATGAAAAGCTAGCTTCCATTAGGATGGATGATTGTTAAAAAAAAAAAAAAAAAAAAAACTATCTCAAATAGGACACAAAGAATCTTCCACTTAGAGTAAGCAGTTGAAGCCATCTGCGGATAAAAAAAGGGTGAAAGATAATCCCTTGATGTGGCCAAACCTTTCATTTAGTTTTTCTATTCAGAAAAGAACCAAGAAGTCCACAGACGAGAGTCATTCTTTAATTCATATTATCATTCTCTGCCAACACATTACCCCTGAGGATTGAATATAATTTGGAACAATACAGCAAAAGCCAGAGGAATTTTTGAGATGATTTTAGAGAGGATAGTTTTGAACCTATTTTCAAACATTTATCAAGAATTTTGTAAGAGCCCATGGCTAGGGAGGCTTGTGGGTCGATTGCCTTTCACTGGTCATCCGTAGCCATAAAGGCAGTATAAATCTTGGAAAATTCTCACCAAGAATTCCTTTCTTAAAGAAGCCTAGATGTTGACATGGTATCATGCTAGAGAGTATCCCACAGGAGTAGAAATAGCCGTTGGAACTTTATGCTAAATAAAAGCCTACAGGAGTAGAGAACCGGCTGTAAAAATAGAATTCCAACAGACCATTGAAGATTATGTCGTCCTCTCTGCTTAGTACGGTAGACTACTGGGGCTTCGACTATTGGAACCTTTGATTTCTTCTGAAACCTATTTTACTACTATGGATGACTTTTATAACTTTTCCATTAAAAATGCTAAAGGGAAAAAGAGATACCCTGAGAAGGTTAAATTCTCTGTTTGGGACGTTGATCTTTCAGATGTCCTCCTTCTCTCTGACTCAGTTATTTAACTAATCAGTTAGCTTCCTTTTACAAATAAGAACTGAACCTGTATCGTAGGTATAGAAATTAACATAAATACTGCTTAATAAGGCTTCTACTCATCTCCTCATACACTATCGTTCTTGGTAGTATTGCACCCAAGATGTGTGACAACTCATTTAAATTTTCAGGCTGGTTACTCGTCTTATTCAAGGGCCCAAGTGCCATCATGATTTCCCTCAATGTTATTCAATTATACATCTTGAAATTGGTGTTCTGAAGGGCGTATACCAAATTATTTTAGAAGTTGATGCTATGTTTTCATGATTGGAGATATTGAAGTTGAGAAAATGCAATTGAGAAACTACTGTTGTAATATTTTAAGCACTCTTGTATTTGACATCTCTAACCGAATTTTCGTGGCTTATTTCAGAGATCGTCGGTAGAGAAATAAGTTACACAGTAACTGCTGAGGTATGTCTTTTAAATCATACACATTAATATGTTATCCCCATGCTTTCTAGGTCTAAGGCACAGTATCCTCTCTGCAACACGACCTTAGCAATGCTTTTAAACTTGAGCATTCTCTTATAATTTCTGCTACTTTTATGATGCTTTCTAGGATTGTTTCTGGATGAATTATTTGTAGATTATGAGAAGGGCTTAAACAACTGTATAATGTTCTCTAGGTCCAGGCAAATCAATAAACCAGACCCTGGAAATTCTTATTCGGTCGAACATCTGGAACTGTGAAAATTTTTAGTATTAGCAAACGTTCATAATGATCTATTATTGATACGATGCTCCCTTATGAGTTGTCTGATATCAAAGAAATGTTTCATTTTGAAATCCTCGGGTGGCAATTTGCAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGTTAGCAAAGGTAGTAGGAGAAAATAAGGTGTATGTATGGTATTCAATTTTGCTTGAGATACAGTCCACTAATATGAAGCTTTGTGATGTTTGGCAGGAAAGGAGAAGATGTGAAAAATGTGGAAATGATGAAGCTTGGTTTGAGAGCAGACAGGTACACTTTACAAACATTAACAAAATTAGAATTGTGGGTTATTTAGGGTTTATTTCTGTCTTTTCTTCCATTAGAAAGAGTAATAAGTTTGAGTGTGGTTTGAATAAAAATGCAGATGAGATCGGCTGATGAAGGCCAGACCACATTTTATACCTGCACTAAATGTCATCATCAGACTAGAGAGAATTGATCTTCCATATAACTTTTTTTAGGATGTCACATTTATTCATCCTTGAATTAGTTGGGTGGTTCCATTGGAATCCAAAAGCTTTAACATGATGAGTTATGTTCTTAAACAATTAAACTTGATCTGTATGGAATGATTAATCTGAGAATATGACAGAAGTAGCAA

mRNA sequence

ATGGTTTCGTTAGGCCCAACCCTATGTAATGGCGTATGGGAAAATAATGGAAAACCAAATTCTCTTGAAGGAAAAAAGAAAAGCAAAACCCTATTTTCTCACCTTCTAGCCCCAGGCCTTCGTGATTTTTCAGACTTACCTTTCTTGCTGGTTCGTCACCCGCCTCCGCCGCTCGTTACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAGGAAGCTATCGGGGAACCGGCAAGCACCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGCGGAACCATGTTGTCGTTCTGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCTAGGCGAAGTGCAAAAGAGATCGTCGGTAGAGAAATAAGTTACACAGTAACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGTTAGCAAAGGAAAGGAGAAGATGTGAAAAATGTGGAAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGCCAGACCACATTTTATACCTGCACTAAATGTCATCATCAGACTAGAGAGAATTGA

Coding sequence (CDS)

ATGGTTTCGTTAGGCCCAACCCTATGTAATGGCGTATGGGAAAATAATGGAAAACCAAATTCTCTTGAAGGAAAAAAGAAAAGCAAAACCCTATTTTCTCACCTTCTAGCCCCAGGCCTTCGTGATTTTTCAGACTTACCTTTCTTGCTGGTTCGTCACCCGCCTCCGCCGCTCGTTACTGGATCTCGCCCCGACAAGTCGTCTCTAGGTAGGAAGCTATCGGGGAACCGGCAAGCACCACTCATGGCTTATTCTCGTGGACATGACTTCTTGTTTTGCAATTTGTGCGGAACCATGTTGTCGTTCTGCTCAACGAAATATGTTGAGTGTCCTTTGTGCAAGTCTAGGCGAAGTGCAAAAGAGATCGTCGGTAGAGAAATAAGTTACACAGTAACTGCTGAGGAAATTAGAAAGCAGTTGGGAATTTCATTAATTGATGAAGAGAAAATGCAGTTAGCAAAGGAAAGGAGAAGATGTGAAAAATGTGGAAATGATGAAGCTTGGTTTGAGAGCAGACAGATGAGATCGGCTGATGAAGGCCAGACCACATTTTATACCTGCACTAAATGTCATCATCAGACTAGAGAGAATTGA

Homology

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BLAST of CSPI02G16700 vs. Swiss-Prot
Match: RPA12_MACMU (DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta GN=ZNRD1 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.9e-11
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 1


Query: 89  DFLFCNLCGTMLSFCSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 148
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCTRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 149 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

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BLAST of CSPI02G16700 vs. Swiss-Prot
Match: RPA12_BOVIN (DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus GN=ZNRD1 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 9.0e-11
Identity = 37/110 (33.64%), Postives = 60/110 (54.55%), Query Frame = 1


Query: 89  DFLFCNLCGTMLSFCSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 148
           D  FC  CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 13  DLDFCPDCGSVLPLPGVQDAVACTRCGFSINVRDFEGKVVKTSVVFNKLGTAMPLSMEEG 72

Query: 149 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 73  PEFQGPVVDRRCSRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 122

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BLAST of CSPI02G16700 vs. Swiss-Prot
Match: RPA12_PANTR (DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes GN=ZNRD1 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-10
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 1


Query: 89  DFLFCNLCGTMLSFCSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 148
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 149 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

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BLAST of CSPI02G16700 vs. Swiss-Prot
Match: RPA12_HUMAN (DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens GN=ZNRD1 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-10
Identity = 37/110 (33.64%), Postives = 61/110 (55.45%), Query Frame = 1


Query: 89  DFLFCNLCGTMLSFCSTK-YVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGISLIDE 148
           D  FC+ CG++L     +  V C  C    + ++  G+ +  +V   ++   + +S+ + 
Sbjct: 16  DLDFCSDCGSVLPLPGAQDTVTCIRCGFNINVRDFEGKVVKTSVVFHQLGTAMPMSVEEG 75

Query: 149 EKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
            + Q     RRC +CG++   + +RQMRSADEGQT FYTCT C  Q +E+
Sbjct: 76  PECQGPVVDRRCPRCGHEGMAYHTRQMRSADEGQTVFYTCTNCKFQEKED 125

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BLAST of CSPI02G16700 vs. Swiss-Prot
Match: RPA12_SCHPO (DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=rpa12 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.4e-10
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 1


Query: 91  LFCNLCGTMLSFCSTKYVECPLCKS----RRSAKEIVGREISYTVTAEEIRKQLGISLID 150
           +FC+ CG +L   + ++  C  C+S     + A  +V  + S +     ++ +  I  ++
Sbjct: 8   IFCSECGNLLESTTAQWTTCDQCQSVYPSEQFANLVVETKSSASAFPSALKLKHSIVQVE 67

Query: 151 EEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
            +K + A    +C KCGND   F + Q+RSADEG T FY C +C ++   N
Sbjct: 68  SQKEEAATIEEKCPKCGNDHMTFHTLQLRSADEGSTVFYECPRCAYKFSTN 118

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BLAST of CSPI02G16700 vs. TrEMBL
Match: A0A0A0LKC4_CUCSA (DNA-directed RNA polymerase subunit OS=Cucumis sativus GN=Csa_2G345940 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 2.5e-60
Identity = 115/116 (99.14%), Postives = 115/116 (99.14%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYSRGHDFLFCNLCGTMLSFCSTKYVECP CKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

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BLAST of CSPI02G16700 vs. TrEMBL
Match: B3FNK6_CUCSA (DNA-directed RNA polymerase subunit OS=Cucumis sativus PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.6e-54
Identity = 108/116 (93.10%), Postives = 110/116 (94.83%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYSRGHDFLFCNL GTMLSFCSTKYVECP CKSRRSAKEIVGREISYTVTAEEI+KQLG
Sbjct: 1   MAYSRGHDFLFCNLGGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIKKQLG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           ISLIDE  MQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTC+KC  QTREN
Sbjct: 61  ISLIDENNMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCSKCLPQTREN 116

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BLAST of CSPI02G16700 vs. TrEMBL
Match: G7JQD3_MEDTR (DNA-directed RNA polymerase subunit OS=Medicago truncatula GN=MTR_3g102420 PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.7e-37
Identity = 75/114 (65.79%), Postives = 96/114 (84.21%), Query Frame = 1


Query: 84  YSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGIS 143
           YSRG DFLFC+LCGTML+  ST Y  CPLCK++ + K+I G+EISYT++AE+IR++LGI 
Sbjct: 2   YSRGRDFLFCHLCGTMLTVPSTDYALCPLCKTKCNIKDIKGKEISYTISAEDIRRELGID 61

Query: 144 LIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           LI+E+K+QL+K  + CEKCG+ EA F +RQMRSADEGQTTFYTCT+C HQ +EN
Sbjct: 62  LIEEQKVQLSKVNKTCEKCGHGEAAFYTRQMRSADEGQTTFYTCTRCGHQFQEN 115

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BLAST of CSPI02G16700 vs. TrEMBL
Match: A0A151TUW7_CAJCA (DNA-directed RNA polymerase subunit OS=Cajanus cajan GN=KK1_010091 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 9.6e-36
Identity = 75/116 (64.66%), Postives = 99/116 (85.34%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MA SR  DFLFCNLCGT+L+  ST+Y +CPLCK+RR+ + I  REISYT+ AE+IR++LG
Sbjct: 1   MASSR-LDFLFCNLCGTLLTVPSTEYAQCPLCKTRRNIQHIRDREISYTIYAEDIRRELG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           + +I+E+K+QL+K  ++CEKCG+DEA F +RQMRSADEGQTTFYTCT+C HQ++EN
Sbjct: 61  MEVIEEQKVQLSKVNKKCEKCGHDEANFYTRQMRSADEGQTTFYTCTRCGHQSQEN 115

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BLAST of CSPI02G16700 vs. TrEMBL
Match: V7CX97_PHAVU (DNA-directed RNA polymerase subunit OS=Phaseolus vulgaris GN=PHAVU_001G175600g PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.4e-33
Identity = 71/116 (61.21%), Postives = 96/116 (82.76%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MA SR  DFLFC+LCGTML+  +++Y  CPLCK+ R  ++I  +EI YT+ AE+IR++LG
Sbjct: 1   MASSR-LDFLFCHLCGTMLTVPNSEYAVCPLCKTLRDIQDIRDKEIGYTIYAEDIRRELG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           I +I+E+K+QL+K  ++CEKCG+DEA F +RQMRSADEGQTTFYTCT+C HQ++EN
Sbjct: 61  IEIIEEQKVQLSKVNKKCEKCGHDEANFYTRQMRSADEGQTTFYTCTRCGHQSQEN 115

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BLAST of CSPI02G16700 vs. TAIR10
Match: AT3G25940.1 (AT3G25940.1 TFIIB zinc-binding protein)

HSP 1 Score: 133.3 bits (334), Expect = 1.7e-31
Identity = 66/118 (55.93%), Postives = 87/118 (73.73%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           M  SR  +FLFCNLCGTML   STKY ECP CK+ R+AK+I+ +EI+YTV+AE+IR++LG
Sbjct: 1   MEKSRESEFLFCNLCGTMLVLKSTKYAECPHCKTTRNAKDIIDKEIAYTVSAEDIRRELG 60

Query: 142 ISLIDEE---KMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 197
           ISL  E+   + +L K ++ CEKC + E  + +RQ RSADEGQTT+YTC  C H+  E
Sbjct: 61  ISLFGEKTQAEAELPKIKKACEKCQHPELVYTTRQTRSADEGQTTYYTCPNCAHRFTE 118

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BLAST of CSPI02G16700 vs. TAIR10
Match: AT1G20065.2 (AT1G20065.2 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 72.0 bits (175), Expect = 4.6e-13
Identity = 32/53 (60.38%), Postives = 39/53 (73.58%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAE 135
           M  SR  D LFCNLCGTML   STKY ECPLC++ R+ KEI+ + ++YTVT E
Sbjct: 1   MEKSRESDILFCNLCGTMLVLKSTKYAECPLCETTRNGKEIIDKNLAYTVTTE 53

[Close]

BLAST of CSPI02G16700 vs. TAIR10
Match: AT1G01210.1 (AT1G01210.1 DNA-directed RNA polymerase, subunit M, archaeal)

HSP 1 Score: 53.1 bits (126), Expect = 2.2e-07
Identity = 35/114 (30.70%), Postives = 55/114 (48.25%), Query Frame = 1


Query: 92  FCNLCGTMLS---------FCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGI 151
           FC  CG +L          FCST    CP     +   EI  +++        ++K +  
Sbjct: 3   FCPTCGNLLRYEGGGNSRFFCST----CPYVAYIQRQVEIKKKQLL-------VKKSIEA 62

Query: 152 SLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 197
            +  ++    A+    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  VVTKDDIPTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]

BLAST of CSPI02G16700 vs. TAIR10
Match: AT4G07950.1 (AT4G07950.1 DNA-directed RNA polymerase, subunit M, archaeal)

HSP 1 Score: 53.1 bits (126), Expect = 2.2e-07
Identity = 35/114 (30.70%), Postives = 55/114 (48.25%), Query Frame = 1


Query: 92  FCNLCGTMLS---------FCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLGI 151
           FC  CG +L          FCST    CP   +     EI  +++        ++K +  
Sbjct: 3   FCPTCGNLLRYEGGGSSRFFCST----CPYVANIERRVEIKKKQLL-------VKKSIEP 62

Query: 152 SLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTRE 197
            +  ++    A+    C +CG+D+A+F+S Q+RSADE ++ FY C KC    RE
Sbjct: 63  VVTKDDIPTAAETEAPCPRCGHDKAYFKSMQIRSADEPESRFYRCLKCEFTWRE 105

[Close]

BLAST of CSPI02G16700 vs. NCBI nr
Match: gi|449450514|ref|XP_004143007.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus])

HSP 1 Score: 240.0 bits (611), Expect = 3.6e-60
Identity = 115/116 (99.14%), Postives = 115/116 (99.14%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYSRGHDFLFCNLCGTMLSFCSTKYVECP CKSRRSAKEIVGREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIRKQLG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of CSPI02G16700 vs. NCBI nr
Match: gi|659089115|ref|XP_008445334.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12-like [Cucumis melo])

HSP 1 Score: 235.7 bits (600), Expect = 6.8e-59
Identity = 113/116 (97.41%), Postives = 114/116 (98.28%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKS+RSAKEI GREISYTVTAEEIRKQLG
Sbjct: 1   MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSKRSAKEINGREISYTVTAEEIRKQLG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           ISLI EEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN
Sbjct: 61  ISLIGEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 116

[Close]

BLAST of CSPI02G16700 vs. NCBI nr
Match: gi|821595252|ref|NP_001295812.1| (DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus])

HSP 1 Score: 220.7 bits (561), Expect = 2.3e-54
Identity = 108/116 (93.10%), Postives = 110/116 (94.83%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYSRGHDFLFCNL GTMLSFCSTKYVECP CKSRRSAKEIVGREISYTVTAEEI+KQLG
Sbjct: 1   MAYSRGHDFLFCNLGGTMLSFCSTKYVECPSCKSRRSAKEIVGREISYTVTAEEIKKQLG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           ISLIDE  MQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTC+KC  QTREN
Sbjct: 61  ISLIDENNMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCSKCLPQTREN 116

[Close]

BLAST of CSPI02G16700 vs. NCBI nr
Match: gi|1009135164|ref|XP_015884839.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 180.3 bits (456), Expect = 3.4e-42
Identity = 83/116 (71.55%), Postives = 103/116 (88.79%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYS G DFLFCNLCGT+L+F STK+ +CPLCKS++  K+I+GREISYTVTAE+IR++LG
Sbjct: 1   MAYSHGRDFLFCNLCGTVLAFGSTKHAQCPLCKSKQKLKDIIGREISYTVTAEDIRRELG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           I LI EEK++L+K +RRCEKCG+DEA + +RQMRSADEGQTTFYTCTKC HQ ++N
Sbjct: 61  IVLIGEEKVELSKIKRRCEKCGHDEASYYTRQMRSADEGQTTFYTCTKCQHQFQDN 116

[Close]

BLAST of CSPI02G16700 vs. NCBI nr
Match: gi|1009135166|ref|XP_015884840.1| (PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X2 [Ziziphus jujuba])

HSP 1 Score: 176.4 bits (446), Expect = 4.9e-41
Identity = 82/116 (70.69%), Postives = 102/116 (87.93%), Query Frame = 1


Query: 82  MAYSRGHDFLFCNLCGTMLSFCSTKYVECPLCKSRRSAKEIVGREISYTVTAEEIRKQLG 141
           MAYS G DFLFCNL GT+L+F STK+ +CPLCKS++  K+I+GREISYTVTAE+IR++LG
Sbjct: 1   MAYSHGRDFLFCNLVGTVLAFGSTKHAQCPLCKSKQKLKDIIGREISYTVTAEDIRRELG 60

Query: 142 ISLIDEEKMQLAKERRRCEKCGNDEAWFESRQMRSADEGQTTFYTCTKCHHQTREN 198
           I LI EEK++L+K +RRCEKCG+DEA + +RQMRSADEGQTTFYTCTKC HQ ++N
Sbjct: 61  IVLIGEEKVELSKIKRRCEKCGHDEASYYTRQMRSADEGQTTFYTCTKCQHQFQDN 116

The following BLAST results are available for this feature:

BLAST of CSPI02G16700 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RPA12_MACMU	6.9e-11	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Macaca mulatta GN=ZNRD1 PE=3 SV=1	[more]
RPA12_BOVIN	9.0e-11	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Bos taurus GN=ZNRD1 PE=2 SV=1	[more]
RPA12_PANTR	1.2e-10	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Pan troglodytes GN=ZNRD1 PE=3 SV=...	[more]
RPA12_HUMAN	1.2e-10	33.64	DNA-directed RNA polymerase I subunit RPA12 OS=Homo sapiens GN=ZNRD1 PE=2 SV=1	[more]
RPA12_SCHPO	3.4e-10	30.63	DNA-directed RNA polymerase I subunit RPA12 OS=Schizosaccharomyces pombe (strain...	[more]

BLAST of CSPI02G16700 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKC4_CUCSA	2.5e-60	99.14	DNA-directed RNA polymerase subunit OS=Cucumis sativus GN=Csa_2G345940 PE=3 SV=1	[more]
B3FNK6_CUCSA	1.6e-54	93.10	DNA-directed RNA polymerase subunit OS=Cucumis sativus PE=2 SV=1	[more]
G7JQD3_MEDTR	8.7e-37	65.79	DNA-directed RNA polymerase subunit OS=Medicago truncatula GN=MTR_3g102420 PE=3 ...	[more]
A0A151TUW7_CAJCA	9.6e-36	64.66	DNA-directed RNA polymerase subunit OS=Cajanus cajan GN=KK1_010091 PE=3 SV=1	[more]
V7CX97_PHAVU	3.4e-33	61.21	DNA-directed RNA polymerase subunit OS=Phaseolus vulgaris GN=PHAVU_001G175600g P...	[more]

BLAST of CSPI02G16700 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G25940.1	1.7e-31	55.93	TFIIB zinc-binding protein	[more]
AT1G20065.2	4.6e-13	60.38	FUNCTIONS IN: molecular_function unknown	[more]
AT1G01210.1	2.2e-07	30.70	DNA-directed RNA polymerase, subunit M, archaeal	[more]
AT4G07950.1	2.2e-07	30.70	DNA-directed RNA polymerase, subunit M, archaeal	[more]

BLAST of CSPI02G16700 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450514\|ref\|XP_004143007.1\|	3.6e-60	99.14	PREDICTED: DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus]	[more]
gi\|659089115\|ref\|XP_008445334.1\|	6.8e-59	97.41	PREDICTED: DNA-directed RNA polymerase I subunit RPA12-like [Cucumis melo]	[more]
gi\|821595252\|ref\|NP_001295812.1\|	2.3e-54	93.10	DNA-directed RNA polymerase I subunit RPA12 [Cucumis sativus]	[more]
gi\|1009135164\|ref\|XP_015884839.1\|	3.4e-42	71.55	PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X1 [Ziziphus juju...	[more]
gi\|1009135166\|ref\|XP_015884840.1\|	4.9e-41	70.69	PREDICTED: DNA-directed RNA polymerase I subunit RPA12 isoform X2 [Ziziphus juju...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001222	Znf_TFIIS

Vocabulary: **Biological Process**
Term	Definition
GO:0006351	transcription, DNA-templated

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006379	mRNA cleavage
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003899	DNA-directed RNA polymerase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G16700.1	CSPI02G16700.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001222	Zinc finger, TFIIS-type	PFAM	PF01096	TFIIS_C	coord: 158..193 score: 2.0
IPR001222	Zinc finger, TFIIS-type	SMART	SM00440	Cys4_2	coord: 157..196 score: 3.7
IPR001222	Zinc finger, TFIIS-type	PROFILE	PS51133	ZF_TFIIS_2	coord: 155..195 score: 12
None	No IPR available	GENE3D	G3DSA:2.20.25.10		coord: 147..197 score: 5.1
None	No IPR available	PANTHER	PTHR11239	DNA-DIRECTED RNA POLYMERASE	coord: 89..197 score: 8.3
None	No IPR available	PANTHER	PTHR11239:SF14	DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA12	coord: 89..197 score: 8.3
None	No IPR available	unknown	SSF57783	Zinc beta-ribbon	coord: 136..197 score: 9.64

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G16700	Csa2G345940	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G16700	MELO3C011355	Melon (DHL92) v3.5.1	cpimeB117
CSPI02G16700	ClCG08G014170	Watermelon (Charleston Gray)	cpiwcgB173
CSPI02G16700	Lsi08G013060	Bottle gourd (USVL1VR-Ls)	cpilsiB142
CSPI02G16700	MELO3C011355.2	Melon (DHL92) v3.6.1	cpimedB113
CSPI02G16700	CsaV3_2G024260	Cucumber (Chinese Long) v3	cpicucB081
CSPI02G16700	Cla97C08G157730	Watermelon (97103) v2	cpiwmbB167
CSPI02G16700	Bhi04G000106	Wax gourd	cpiwgoB163
CSPI02G16700	Cucsa.393870	Cucumber (Gy14) v1	cgycpiB578
CSPI02G16700	CmaCh05G003170	Cucurbita maxima (Rimu)	cmacpiB797
CSPI02G16700	CmoCh05G003200	Cucurbita moschata (Rifu)	cmocpiB785
CSPI02G16700	Cp4.1LG11g02720	Cucurbita pepo (Zucchini)	cpecpiB096
CSPI02G16700	CsGy2G016690	Cucumber (Gy14) v2	cgybcpiB057
CSPI02G16700	Carg09395	Silver-seed gourd	carcpiB0158

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G16700	Cucurbita moschata (Rifu)	cmocpiB163
CSPI02G16700	Watermelon (97103) v1	cpiwmB117
CSPI02G16700	Cucurbita pepo (Zucchini)	cpecpiB825
CSPI02G16700	Silver-seed gourd	carcpiB1013