Cucsa.366410 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.366410
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCysteine proteinase
Locationscaffold03625 : 44764 .. 45849 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGTATGAACTTGTAATACTCTCATTCATTCAAATTATCAGAAATACGTTGAACATGTTCTTTTTACGTCTGATTATAAGTTTTTTTTTTCCTATTCCTAACAAGTACCCTAAACCCTACCTTCTATCCATTGTAAGTCATTTCATCGTATTTGGAAAAACTTACCAATCTTGAATTTTCGACACAGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAGCAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

mRNA sequence

GTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAGCAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

Coding sequence (CDS)

GTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAGCAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

Protein sequence

YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD*
Homology
[Close]
BLAST of Cucsa.366410 vs. Swiss-Prot
Match: CEP1_ARATH (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 171/309 (55.34%), Postives = 214/309 (69.26%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W   +    +S EE  +RF +++ NV++I   N  + S+ L  N F D+T+EEF+ T
Sbjct: 38  YERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRT 97

Query: 61  YLG--------YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 120
           Y G        ++        F Y N+  LPT+VDWR+ GAVTP+KNQGQCGSCWAFS V
Sbjct: 98  YAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTV 157

Query: 121 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTTEIEYPY 180
            AVEGIN+I+  KL SLSEQELVDCD T+ NQGCNGG M  AFEFIK + GLT+E+ YPY
Sbjct: 158 VAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPY 217

Query: 181 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 240
           + ++  C+  KE    VSI G+E VP N E  L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 218 KASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGV 277

Query: 241 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 300
           F+G CG +LNHGVA+VGYG T     YW+VKNSWG +WGE GYIRM+R    K+G CGIA
Sbjct: 278 FTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIA 337

[Close]
BLAST of Cucsa.366410 vs. Swiss-Prot
Match: XCP1_ARATH (Cysteine protease XCP1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-92
Identity = 168/304 (55.26%), Postives = 214/304 (70.39%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           ++ WM ++ + YKS EE   RF +++ N+ +ID  N+  +S+ L  N FADLT+EEFK  
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGR 110

Query: 61  YLG-----YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAV 120
           YLG     +     P   FRY ++ +LP +VDWR++GAV P+K+QGQCGSCWAFS VAAV
Sbjct: 111 YLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAV 170

Query: 121 EGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGA 180
           EGIN+I  G L SLSEQEL+DCD T+ N GCNGG M  AF++I  T GL  E +YPY   
Sbjct: 171 EGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLME 230

Query: 181 ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSG 240
           E  C EQKE  + V+ISGYE VP ND++SL  A+A+QPVSVAI+A G +FQFY GG+F+G
Sbjct: 231 EGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNG 290

Query: 241 NCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMAS 299
            CG  L+HGVA VGYG +    Y +VKNSWG  WGE G+IRMKR++   +G CGI  MAS
Sbjct: 291 KCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMAS 350

[Close]
BLAST of Cucsa.366410 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.2e-92
Identity = 170/309 (55.02%), Postives = 214/309 (69.26%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W   +    +S  E  +RF +++ANV ++ N N M+  + L  N FAD+TN EF++T
Sbjct: 40  YERWRSHHTVS-RSLGEKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRST 99

Query: 61  YLGYKT--------VSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 120
           Y G K                F Y  + ++P +VDWR++GAVT +K+QGQCGSCWAFS +
Sbjct: 100 YAGSKVNHHKMFRGSQHGSGTFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTI 159

Query: 121 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYPY 180
            AVEGIN+IK  KL+SLSEQELVDCD    NQGCNGG M  AFEFIK+ G +TTE  YPY
Sbjct: 160 VAVEGINQIKTNKLVSLSEQELVDCDKEE-NQGCNGGLMESAFEFIKQKGGITTESNYPY 219

Query: 181 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 240
              E  C+E K     VSI G+E VPVNDE +L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 220 TAQEGTCDESKVNDLAVSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGV 279

Query: 241 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 300
           F+G+C   LNHGVAIVGYG T     YW+V+NSWG +WGE GYIRM+R+ + K+G CGIA
Sbjct: 280 FTGDCNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIA 339

[Close]
BLAST of Cucsa.366410 vs. Swiss-Prot
Match: SAG12_ARATH (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana GN=SAG12 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.4e-92
Identity = 170/308 (55.19%), Postives = 220/308 (71.43%), Query Frame = 1 

Query: 3   KWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHT--LAENNFADLTNEEFKAT 62
           +WM K+GR Y   +E   R+ +++ NV+ I++ NS+    T  LA N FADLTN+EF++ 
Sbjct: 40  EWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSM 99

Query: 63  YLGYKTVSIPDTC-------FRYGNMVN--LPTNVDWRQEGAVTPIKNQGQCGSCWAFSA 122
           Y G+K VS   +        FRY N+ +  LP +VDWR++GAVTPIKNQG CG CWAFSA
Sbjct: 100 YTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSA 159

Query: 123 VAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYP 182
           VAA+EG  +IK GKLISLSEQ+LVDCD  + + GC GG M  AFE IK TG LTTE  YP
Sbjct: 160 VAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLTTESNYP 219

Query: 183 YQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGG 242
           Y+G ++ CN +K   +  SI+GYE VPVNDE++L  AVA+QPVSV I+  G +FQFYS G
Sbjct: 220 YKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSG 279

Query: 243 IFSGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGI 298
           +F+G C   L+H V  +GYGE++N   YW++KNSWGT WGESGY+R+++D  DKQG CG+
Sbjct: 280 VFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGL 339

[Close]
BLAST of Cucsa.366410 vs. Swiss-Prot
Match: SAG39_ORYSI (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica GN=OsI_14861 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.1e-91
Identity = 171/306 (55.88%), Postives = 215/306 (70.26%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFK-- 60
           +++WM +YGR Y+   E  RRF +++ANV +I++FN+ NH+  L  N FADLTN+EF+  
Sbjct: 37  HERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFADLTNDEFRWT 96

Query: 61  ATYLGY--KTVSIPDTCFRYGNMVN---LPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVA 120
            T  G+   T  +P T FRY N VN   LP  VDWR +GAVTPIK+QGQCG CWAFSAVA
Sbjct: 97  KTNKGFIPSTTRVP-TGFRYEN-VNIDALPATVDWRTKGAVTPIKDQGQCGCCWAFSAVA 156

Query: 121 AVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEF-IKRTGLTTEIEYPYQ 180
           A+EGI K+  GKLISLSEQELVDCDV   +QGC GG M  AF+F IK  GLTTE  YPY 
Sbjct: 157 AMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLTTESNYPYA 216

Query: 181 GAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIF 240
            A+  C          SI GYE VP N+E +L  AVANQPVSVA+D     FQFY GG+ 
Sbjct: 217 AADDKCKSVSN--SVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDMTFQFYKGGVM 276

Query: 241 SGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAM 298
           +G+CG  L+HG+  +GYG+ S+   YWL+KNSWGT WGE+G++RM++D +DK+G CG+AM
Sbjct: 277 TGSCGTDLDHGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKDISDKRGMCGLAM 336

[Close]
BLAST of Cucsa.366410 vs. TrEMBL
Match: A0A0A0LJV6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 8.5e-177
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT
Sbjct: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK
Sbjct: 102 YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 161

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE
Sbjct: 162 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 221

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
           QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL
Sbjct: 222 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 281

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 282 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]
BLAST of Cucsa.366410 vs. TrEMBL
Match: M5X1M2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.2e-115
Identity = 196/298 (65.77%), Postives = 234/298 (78.52%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W+ KYGR YK+REE   RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T
Sbjct: 45  YERWLQKYGRIYKNREEAAYRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKT 104

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           ++G++T S P T F Y     LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+
Sbjct: 105 FMGFQTRSHPKTKFSYDKDEELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQ 164

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK GKL+SLSEQELVDCDV +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E
Sbjct: 165 IKTGKLVSLSEQELVDCDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDE 224

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
              K   V+ISGYE +P N EKSL+AAVA+QPVSVA+DA G  FQFYS G F+G CG  L
Sbjct: 225 DSLKNSAVNISGYESIPANSEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNL 284

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           NHGV  VGYGE S + YW+VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 285 NHGVTAVGYGEDSGKKYWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

[Close]
BLAST of Cucsa.366410 vs. TrEMBL
Match: W9RAD3_9ROSA (KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 8.1e-111
Identity = 188/299 (62.88%), Postives = 230/299 (76.92%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y +W ++YGR Y S EE E RF IY  N+ +I+  NS N S+ L +N FAD+ N EF+  
Sbjct: 48  YDRWAEQYGRNYGSEEEKELRFQIYHMNLLFIEQVNSQNFSYKLTDNKFADMMNAEFRLR 107

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
            LGY+ +    T FR+G  + +P  VDWR+ GAVTP+K+QGQCGSCWAFS+VAAVEG+N+
Sbjct: 108 LLGYRPLLHNQTSFRFGGPMLVPKQVDWRKNGAVTPVKDQGQCGSCWAFSSVAAVEGVNQ 167

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK G+L+SLSEQELVDCDV +GNQGCNGGYM KAF+FIKR G+TT  +YPY+GA   C+E
Sbjct: 168 IKTGELVSLSEQELVDCDVNTGNQGCNGGYMEKAFQFIKRNGITTNGKYPYRGANGRCDE 227

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
            K + + V ISGYEKVP NDE+ L+A VA+QPVSVAIDA G+ FQFYS GIF+G CG  L
Sbjct: 228 DKLRGRRVKISGYEKVPHNDEERLQATVAHQPVSVAIDAGGSEFQFYSHGIFNGRCGTDL 287

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGV +VGYGE   + YWLVKNSWGT+WGESGY+R+ R S D +GTCGIAM ASYP KD
Sbjct: 288 NHGVTVVGYGEEDGKTYWLVKNSWGTEWGESGYVRIHRGSVDGRGTCGIAMEASYPVKD 346

[Close]
BLAST of Cucsa.366410 vs. TrEMBL
Match: G7ZUL7_MEDTR (Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.4e-110
Identity = 189/299 (63.21%), Postives = 230/299 (76.92%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y+ W+ +YGR Y+ REEWE RF IYQ+NVQYI+ +NS N+S+ L +N FAD+TNEEFK+T
Sbjct: 39  YETWLKRYGRHYRDREEWEVRFDIYQSNVQYIEFYNSQNYSYKLIDNRFADITNEEFKST 98

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGY       T FRY     LP ++DWR++GAVT +K+QG+CGSCWAFSAVAAVEGINK
Sbjct: 99  YLGYLPRFRVQTEFRYHKHGELPKSIDWRKKGAVTHVKDQGRCGSCWAFSAVAAVEGINK 158

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKR-TGLTTEIEYPYQGAESACN 180
           IK   L+SLSEQ+L+DCD+ SGN+GC GG MY AF +IK+  G+ T  EYPY+G +  CN
Sbjct: 159 IKTENLVSLSEQQLIDCDIKSGNEGCEGGDMYIAFNYIKKHGGIATAKEYPYKGRDGNCN 218

Query: 181 EQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQ 240
           + K K   V+ISGYE VP  +EK LKAAVA+QPVS+A DA G  FQFYS GIFSG+CG  
Sbjct: 219 KSKAKNNAVTISGYESVPARNEKMLKAAVAHQPVSIATDAGGYAFQFYSKGIFSGSCGKN 278

Query: 241 LNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           LNHG+ IVGYGE +   YW+VKNSW  DWGESGY+RMKRD+ DK GTCGIAM A+YP K
Sbjct: 279 LNHGMTIVGYGEENGDKYWIVKNSWANDWGESGYVRMKRDTKDKDGTCGIAMDATYPVK 337

[Close]
BLAST of Cucsa.366410 vs. TrEMBL
Match: A0A151TPE0_CAJCA (Vignain OS=Cajanus cajan GN=KK1_022504 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-107
Identity = 190/301 (63.12%), Postives = 231/301 (76.74%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           +++W+ ++GR YK +EEWE RF IYQAN+++I+  NS N+S+ L +N FADLTNEEF +T
Sbjct: 5   FERWITQHGRNYKDKEEWEVRFGIYQANLKFIECKNSQNNSYNLIDNKFADLTNEEFMST 64

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLG+ T     T        +LP ++DWR+EGAVT IK+QG CGSCWAFSAVAAVEGINK
Sbjct: 65  YLGFGTRLPTHTGIGSYKHGDLPESMDWRKEGAVTEIKDQGNCGSCWAFSAVAAVEGINK 124

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGAESACN 180
           IK+GKL+SLSEQEL+DCDV +GNQGC GG M  AF+FIKR  GL T  EYPY+G +  CN
Sbjct: 125 IKSGKLVSLSEQELIDCDVENGNQGCEGGLMDTAFKFIKRNGGLATAKEYPYEGVDGTCN 184

Query: 181 EQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQ 240
           ++K  +  V+ISGYE+VP N+E  LKAAVANQPVSVAIDA G  FQ YS G+FSG CG  
Sbjct: 185 KEKAGHHAVNISGYERVPSNNEAMLKAAVANQPVSVAIDAGGYEFQLYSEGVFSGRCGKH 244

Query: 241 LNHGVAIVGYGETS-NQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 300
           LNHGV +VGYGE +    YW+VKNSWG DWGESGY+RMKRD+ D  G CGIAM ASYP K
Sbjct: 245 LNHGVTVVGYGEENIGDKYWIVKNSWGLDWGESGYVRMKRDTVDDAGICGIAMQASYPLK 304

[Close]
BLAST of Cucsa.366410 vs. TAIR10
Match: AT1G06260.1 (AT1G06260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-96
Identity = 172/303 (56.77%), Postives = 212/303 (69.97%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           ++KW+  + + Y  R+EW  RF IYQ+NVQ ID  NS++    L +N FAD+TN EFKA 
Sbjct: 43  FEKWLKTHSKLYGGRDEWMLRFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAH 102

Query: 61  YLGYKTVSIP------DTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAA 120
           +LG  T S+         C   GN+   P  VDWR +GAVTPI+NQG+CG CWAFSAVAA
Sbjct: 103 FLGLNTSSLRLHKKQRPVCDPAGNV---PDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAA 162

Query: 121 VEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYPYQG 180
           +EGINKIK G L+SLSEQ+L+DCDV + N+GC+GG M  AFEFIK  G L TE +YPY G
Sbjct: 163 IEGINKIKTGNLVSLSEQQLIDCDVGTYNKGCSGGLMETAFEFIKTNGGLATETDYPYTG 222

Query: 181 AESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFS 240
            E  C+++K K + V+I GY+KV  N E SL+ A A QPVSV IDA G  FQ YS G+F+
Sbjct: 223 IEGTCDQEKSKNKVVTIQGYQKVAQN-EASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFT 282

Query: 241 GNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMA 297
             CG  LNHGV +VGYG   +Q YW+VKNSWGT WGE GYIRM+R  ++  G CGIAMMA
Sbjct: 283 NYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMA 341

[Close]
BLAST of Cucsa.366410 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 340.5 bits (872), Expect = 1.1e-93
Identity = 171/309 (55.34%), Postives = 214/309 (69.26%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W   +    +S EE  +RF +++ NV++I   N  + S+ L  N F D+T+EEF+ T
Sbjct: 38  YERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRT 97

Query: 61  YLG--------YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 120
           Y G        ++        F Y N+  LPT+VDWR+ GAVTP+KNQGQCGSCWAFS V
Sbjct: 98  YAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTV 157

Query: 121 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTTEIEYPY 180
            AVEGIN+I+  KL SLSEQELVDCD T+ NQGCNGG M  AFEFIK + GLT+E+ YPY
Sbjct: 158 VAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPY 217

Query: 181 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 240
           + ++  C+  KE    VSI G+E VP N E  L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 218 KASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGV 277

Query: 241 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 300
           F+G CG +LNHGVA+VGYG T     YW+VKNSWG +WGE GYIRM+R    K+G CGIA
Sbjct: 278 FTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIA 337

[Close]
BLAST of Cucsa.366410 vs. TAIR10
Match: AT4G35350.1 (AT4G35350.1 xylem cysteine peptidase 1)

HSP 1 Score: 340.1 bits (871), Expect = 1.4e-93
Identity = 168/304 (55.26%), Postives = 214/304 (70.39%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           ++ WM ++ + YKS EE   RF +++ N+ +ID  N+  +S+ L  N FADLT+EEFK  
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGR 110

Query: 61  YLG-----YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAV 120
           YLG     +     P   FRY ++ +LP +VDWR++GAV P+K+QGQCGSCWAFS VAAV
Sbjct: 111 YLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAV 170

Query: 121 EGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGA 180
           EGIN+I  G L SLSEQEL+DCD T+ N GCNGG M  AF++I  T GL  E +YPY   
Sbjct: 171 EGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLME 230

Query: 181 ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSG 240
           E  C EQKE  + V+ISGYE VP ND++SL  A+A+QPVSVAI+A G +FQFY GG+F+G
Sbjct: 231 EGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNG 290

Query: 241 NCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMAS 299
            CG  L+HGVA VGYG +    Y +VKNSWG  WGE G+IRMKR++   +G CGI  MAS
Sbjct: 291 KCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMAS 350

[Close]
BLAST of Cucsa.366410 vs. TAIR10
Match: AT5G45890.1 (AT5G45890.1 senescence-associated gene 12)

HSP 1 Score: 339.0 bits (868), Expect = 3.1e-93
Identity = 170/308 (55.19%), Postives = 220/308 (71.43%), Query Frame = 1 

Query: 3   KWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHT--LAENNFADLTNEEFKAT 62
           +WM K+GR Y   +E   R+ +++ NV+ I++ NS+    T  LA N FADLTN+EF++ 
Sbjct: 40  EWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSM 99

Query: 63  YLGYKTVSIPDTC-------FRYGNMVN--LPTNVDWRQEGAVTPIKNQGQCGSCWAFSA 122
           Y G+K VS   +        FRY N+ +  LP +VDWR++GAVTPIKNQG CG CWAFSA
Sbjct: 100 YTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSA 159

Query: 123 VAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYP 182
           VAA+EG  +IK GKLISLSEQ+LVDCD  + + GC GG M  AFE IK TG LTTE  YP
Sbjct: 160 VAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLTTESNYP 219

Query: 183 YQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGG 242
           Y+G ++ CN +K   +  SI+GYE VPVNDE++L  AVA+QPVSV I+  G +FQFYS G
Sbjct: 220 YKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSG 279

Query: 243 IFSGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGI 298
           +F+G C   L+H V  +GYGE++N   YW++KNSWGT WGESGY+R+++D  DKQG CG+
Sbjct: 280 VFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGL 339

[Close]
BLAST of Cucsa.366410 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 335.1 bits (858), Expect = 4.4e-92
Identity = 166/309 (53.72%), Postives = 214/309 (69.26%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y +W   +    +S  E E+RF +++ NV ++ N N  N S+ L  N FADLT  EFK  
Sbjct: 38  YDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNA 97

Query: 61  YLG-----YKTVSIPDT-----CFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFS 120
           Y G     ++ +  P        + + N+  LP++VDWR++GAVT IKNQG+CGSCWAFS
Sbjct: 98  YTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFS 157

Query: 121 AVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEY 180
            VAAVEGINKIK  KL+SLSEQELVDCD T  N+GCNGG M  AFEFIK+ G +TTE  Y
Sbjct: 158 TVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCNGGLMEIAFEFIKKNGGITTEDSY 217

Query: 181 PYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSG 240
           PY+G +  C+  K+    V+I G+E VP NDE +L  AVANQPVSVAIDA  ++FQFYS 
Sbjct: 218 PYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE 277

Query: 241 GIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGI 299
           G+F+G+CG +LNHGVA VGYG    + YW+V+NSWG +WGE GYI+++R+  + +G CGI
Sbjct: 278 GVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGI 337

[Close]
BLAST of Cucsa.366410 vs. NCBI nr
Match: gi|449460678|ref|XP_004148072.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-176
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT
Sbjct: 19  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 78

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK
Sbjct: 79  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 138

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE
Sbjct: 139 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 198

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
           QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL
Sbjct: 199 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 258

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 259 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 317

[Close]
BLAST of Cucsa.366410 vs. NCBI nr
Match: gi|700206934|gb|KGN62053.1| (hypothetical protein Csa_2G292830 [Cucumis sativus])

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-176
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT
Sbjct: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK
Sbjct: 102 YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 161

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE
Sbjct: 162 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 221

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
           QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL
Sbjct: 222 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 281

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 282 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]
BLAST of Cucsa.366410 vs. NCBI nr
Match: gi|659117224|ref|XP_008458487.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 568.9 bits (1465), Expect = 5.1e-159
Identity = 271/301 (90.03%), Postives = 284/301 (94.35%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWE+RFTIYQANVQYIDNFNS+NHS+TLAENNF DLTNEEF AT
Sbjct: 46  YQKWMDKYGRQYKSREEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMAT 105

Query: 61  YLGYKTVSIPDT--CFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGI 120
           YLGY+TVS+PDT   FRYGNMVNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGI
Sbjct: 106 YLGYETVSLPDTDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGI 165

Query: 121 NKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESAC 180
           NKIKAGKL+SLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK+TGLTTE+EYPY    SAC
Sbjct: 166 NKIKAGKLMSLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSAC 225

Query: 181 NEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGN 240
           ++QKEKYQ VSISGYEKVPVNDEKSL+AAVA QPVSVAIDA GN+FQFYSGGIFSGNCG 
Sbjct: 226 DKQKEKYQSVSISGYEKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGK 285

Query: 241 QLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 300
           QLNHGVAIVGYGE SNQAYWLVKNSWGT WGESGYIRM RDSTDKQGTCGIAMMASYP K
Sbjct: 286 QLNHGVAIVGYGEDSNQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIK 345

[Close]
BLAST of Cucsa.366410 vs. NCBI nr
Match: gi|645245974|ref|XP_008229136.1| (PREDICTED: zingipain-2 [Prunus mume])

HSP 1 Score: 426.0 bits (1094), Expect = 5.4e-116
Identity = 197/298 (66.11%), Postives = 236/298 (79.19%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W+ KYGR YK+REE E RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T
Sbjct: 45  YERWLQKYGRIYKNREEAEYRFGVYKSNIEFVDFVNSQNQSYKLTDNKFADITNLEFTNT 104

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           ++G++T S P T F Y    +LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+
Sbjct: 105 FMGFQTRSHPKTKFSYDKDEDLPTAVDWRKNGAVTPIKNQGQCGSCWAFSAVAAVEGINQ 164

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK GKL+SLSEQELVDCDV +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E
Sbjct: 165 IKTGKLVSLSEQELVDCDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDE 224

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
              K   V+ISGYE +P N EKSL+AAVA+QPVSVA+DA    FQFYS GIF+G CG  L
Sbjct: 225 DSLKNHAVNISGYESIPANSEKSLQAAVAHQPVSVAVDAASYAFQFYSSGIFTGQCGKNL 284

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           NHGV  VGYGE S + YW+VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 285 NHGVTAVGYGEDSGKKYWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

[Close]
BLAST of Cucsa.366410 vs. NCBI nr
Match: gi|595908837|ref|XP_007214244.1| (hypothetical protein PRUPE_ppa023515mg [Prunus persica])

HSP 1 Score: 422.9 bits (1086), Expect = 4.6e-115
Identity = 196/298 (65.77%), Postives = 234/298 (78.52%), Query Frame = 1 

Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W+ KYGR YK+REE   RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T
Sbjct: 45  YERWLQKYGRIYKNREEAAYRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKT 104

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           ++G++T S P T F Y     LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+
Sbjct: 105 FMGFQTRSHPKTKFSYDKDEELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQ 164

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK GKL+SLSEQELVDCDV +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E
Sbjct: 165 IKTGKLVSLSEQELVDCDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDE 224

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
              K   V+ISGYE +P N EKSL+AAVA+QPVSVA+DA G  FQFYS G F+G CG  L
Sbjct: 225 DSLKNSAVNISGYESIPANSEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNL 284

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           NHGV  VGYGE S + YW+VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 285 NHGVTAVGYGEDSGKKYWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

The following BLAST results are available for this feature:
BLAST of Cucsa.366410 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CEP1_ARATH1.9e-9255.34KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=... [more]
XCP1_ARATH2.4e-9255.26Cysteine protease XCP1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1[more]
CYSEP_VIGMU3.2e-9255.02Vignain OS=Vigna mungo PE=1 SV=1[more]
SAG12_ARATH5.4e-9255.19Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana GN=SAG12 PE=... [more]
SAG39_ORYSI2.1e-9155.88Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica GN=OsI... [more]
BLAST of Cucsa.366410 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LJV6_CUCSA8.5e-17799.67Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1[more]
M5X1M2_PRUPE3.2e-11565.77Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1[more]
W9RAD3_9ROSA8.1e-11162.88KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 S... [more]
G7ZUL7_MEDTR2.4e-11063.21Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1[more]
A0A151TPE0_CAJCA1.1e-10763.12Vignain OS=Cajanus cajan GN=KK1_022504 PE=3 SV=1[more]
BLAST of Cucsa.366410 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G06260.11.0e-9656.77 Cysteine proteinases superfamily protein[more]
AT5G50260.11.1e-9355.34 Cysteine proteinases superfamily protein[more]
AT4G35350.11.4e-9355.26 xylem cysteine peptidase 1[more]
AT5G45890.13.1e-9355.19 senescence-associated gene 12[more]
AT3G48340.14.4e-9253.72 Cysteine proteinases superfamily protein[more]
BLAST of Cucsa.366410 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449460678|ref|XP_004148072.1|1.2e-17699.67PREDICTED: ervatamin-B-like [Cucumis sativus][more]
gi|700206934|gb|KGN62053.1|1.2e-17699.67hypothetical protein Csa_2G292830 [Cucumis sativus][more]
gi|659117224|ref|XP_008458487.1|5.1e-15990.03PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|645245974|ref|XP_008229136.1|5.4e-11666.11PREDICTED: zingipain-2 [Prunus mume][more]
gi|595908837|ref|XP_007214244.1|4.6e-11565.77hypothetical protein PRUPE_ppa023515mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000169Pept_cys_AS
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
IPR025660Pept_his_AS
IPR025661Pept_asp_AS
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.366410.1Cucsa.366410.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 100..111
scor
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 242..252
score: 8.4E-11coord: 100..115
score: 8.4E-11coord: 257..263
score: 8.4
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 82..297
score: 3.5
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 82..297
score: 1.7E
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 1..296
score: 5.9E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 1..57
score: 1.1
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 1..57
score: 4.4
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 240..250
scor
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 257..276
scor
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 1..297
score: 6.6E
NoneNo IPR availablePANTHERPTHR12411:SF382CYSTEINE PROTEINASES SUPERFAMILY PROTEINcoord: 1..296
score: 5.9E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 1..297
score: 1.12E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.366410Csa2G292830Cucumber (Chinese Long) v2cgycuB513
Cucsa.366410CSPI02G14740Wild cucumber (PI 183967)cgycpiB542
Cucsa.366410CsaV3_2G017300Cucumber (Chinese Long) v3cgycucB557
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.366410Watermelon (97103) v2cgywmbB635
Cucsa.366410Wax gourdcgywgoB741
Cucsa.366410Cucurbita maxima (Rimu)cgycmaB1015
Cucsa.366410Cucurbita maxima (Rimu)cgycmaB1021
Cucsa.366410Cucurbita moschata (Rifu)cgycmoB1015
Cucsa.366410Cucurbita moschata (Rifu)cgycmoB1020
Cucsa.366410Melon (DHL92) v3.5.1cgymeB596
Cucsa.366410Watermelon (Charleston Gray)cgywcgB629
Cucsa.366410Watermelon (97103) v1cgywmB667
Cucsa.366410Cucurbita pepo (Zucchini)cgycpeB0971
Cucsa.366410Cucurbita pepo (Zucchini)cgycpeB0974
Cucsa.366410Bottle gourd (USVL1VR-Ls)cgylsiB594
Cucsa.366410Melon (DHL92) v3.6.1cgymedB594
Cucsa.366410Silver-seed gourdcarcgyB1111
Cucsa.366410Silver-seed gourdcarcgyB1178