CSPI02G14740 (gene) Wild cucumber (PI 183967)

NameCSPI02G14740
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCysteine proteinase
LocationChr2 : 14419733 .. 14421179 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATGCATACTTACCTCCATTTGCAATCGGAAAAAGTATATATAAAATGACTTGGATTAATGTGAGTTTGATTTTTCTGATTCTCTGGGTTTTCTGGACACCTAGACTGGTATCCATGGCAATGGATTACTCTCTAGGATCTAGTTGTTCCAGTGACATACAAGACAGGTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGTATGAACTTGTAATACTCTCATTCATTCAAATTATCAGAAATACGTTGAACATGTTCTTTTTACGTCTGATTATAAGTTTTTTTTTTCCTATTCCTAACAAGTACCCTAAACCCTACCTTCTATCCATTGTAAGTCATTTCATCGTATTTGGAAAAACTTACCAATCTTGAATTTTCGACACAGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAACAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGAGCATACTTCCTGATGTTAATTGAACTTGTCTACCTTCAGAGGTGAAAAAATTAAATTTGCCATGCAAATTTTTATGTCTACTAAAGATGCAATAAGAATTAGTAAAGGTCATTATGTATAAGCAATAAAAGTCAAAAGAAAATGAAGGATTCAAGTACAATAAAATTTAAACATATTCAAGGAGTATTTATCG

mRNA sequence

ATGACTTGGATTAATGTGAGTTTGATTTTTCTGATTCTCTGGGTTTTCTGGACACCTAGACTGGTATCCATGGCAATGGATTACTCTCTAGGATCTAGTTGTTCCAGTGACATACAAGACAGGTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAACAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

Coding sequence (CDS)

ATGACTTGGATTAATGTGAGTTTGATTTTTCTGATTCTCTGGGTTTTCTGGACACCTAGACTGGTATCCATGGCAATGGATTACTCTCTAGGATCTAGTTGTTCCAGTGACATACAAGACAGGTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAACAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA
BLAST of CSPI02G14740 vs. Swiss-Prot
Match: SAG12_ARATH (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana GN=SAG12 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.1e-91
Identity = 172/314 (54.78%), Postives = 224/314 (71.34%), Query Frame = 1

Query: 38  IQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHT--LAENNFADLTN 97
           +Q R+ +WM K+GR Y   +E   R+ +++ NV+ I++ NS+    T  LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 98  EEFKATYLGYKTVSIPDTC-------FRYGNMVN--LPTNVDWRQEGAVTPIKNQGQCGS 157
           +EF++ Y G+K VS   +        FRY N+ +  LP +VDWR++GAVTPIKNQG CG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGC 153

Query: 158 CWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LT 217
           CWAFSAVAA+EG  +IK GKLISLSEQ+LVDCD  + + GC GG M  AFE IK TG LT
Sbjct: 154 CWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLT 213

Query: 218 TEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNF 277
           TE  YPY+G ++ CN +K   +  SI+GYE VPVNDE++L  AVA+QPVSV I+  G +F
Sbjct: 214 TESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDF 273

Query: 278 QFYSGGIFSGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDK 337
           QFYS G+F+G C   L+H V  +GYGE++N   YW++KNSWGT WGESGY+R+++D  DK
Sbjct: 274 QFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDK 333

Query: 338 QGTCGIAMMASYPT 339
           QG CG+AM ASYPT
Sbjct: 334 QGLCGLAMKASYPT 345

BLAST of CSPI02G14740 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.2e-91
Identity = 171/311 (54.98%), Postives = 215/311 (69.13%), Query Frame = 1

Query: 40  DRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFK 99
           D Y++W   +    +S  E  +RF +++ANV ++ N N M+  + L  N FAD+TN EF+
Sbjct: 38  DLYERWRSHHTVS-RSLGEKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFR 97

Query: 100 ATYLGYKT--------VSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFS 159
           +TY G K                F Y  + ++P +VDWR++GAVT +K+QGQCGSCWAFS
Sbjct: 98  STYAGSKVNHHKMFRGSQHGSGTFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFS 157

Query: 160 AVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEY 219
            + AVEGIN+IK  KL+SLSEQELVDCD    NQGCNGG M  AFEFIK+ G +TTE  Y
Sbjct: 158 TIVAVEGINQIKTNKLVSLSEQELVDCDKEE-NQGCNGGLMESAFEFIKQKGGITTESNY 217

Query: 220 PYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSG 279
           PY   E  C+E K     VSI G+E VPVNDE +L  AVANQPVSVAIDA G++FQFYS 
Sbjct: 218 PYTAQEGTCDESKVNDLAVSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSE 277

Query: 280 GIFSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCG 339
           G+F+G+C   LNHGVAIVGYG T     YW+V+NSWG +WGE GYIRM+R+ + K+G CG
Sbjct: 278 GVFTGDCNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCG 337

Query: 340 IAMMASYPTKD 341
           IAMMASYP K+
Sbjct: 338 IAMMASYPIKN 346

BLAST of CSPI02G14740 vs. Swiss-Prot
Match: CEP1_ARATH (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 8.9e-91
Identity = 171/315 (54.29%), Postives = 217/315 (68.89%), Query Frame = 1

Query: 36  SDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTN 95
           + + + Y++W   +    +S EE  +RF +++ NV++I   N  + S+ L  N F D+T+
Sbjct: 32  NSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTS 91

Query: 96  EEFKATYLG--------YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSC 155
           EEF+ TY G        ++        F Y N+  LPT+VDWR+ GAVTP+KNQGQCGSC
Sbjct: 92  EEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSC 151

Query: 156 WAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTT 215
           WAFS V AVEGIN+I+  KL SLSEQELVDCD T+ NQGCNGG M  AFEFIK + GLT+
Sbjct: 152 WAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTS 211

Query: 216 EIEYPYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQ 275
           E+ YPY+ ++  C+  KE    VSI G+E VP N E  L  AVANQPVSVAIDA G++FQ
Sbjct: 212 ELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQ 271

Query: 276 FYSGGIFSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQ 335
           FYS G+F+G CG +LNHGVA+VGYG T     YW+VKNSWG +WGE GYIRM+R    K+
Sbjct: 272 FYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKE 331

Query: 336 GTCGIAMMASYPTKD 341
           G CGIAM ASYP K+
Sbjct: 332 GLCGIAMEASYPLKN 344

BLAST of CSPI02G14740 vs. Swiss-Prot
Match: XCP1_ARATH (Cysteine protease XCP1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-90
Identity = 168/304 (55.26%), Postives = 214/304 (70.39%), Query Frame = 1

Query: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101
           ++ WM ++ + YKS EE   RF +++ N+ +ID  N+  +S+ L  N FADLT+EEFK  
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGR 110

Query: 102 YLG-----YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAV 161
           YLG     +     P   FRY ++ +LP +VDWR++GAV P+K+QGQCGSCWAFS VAAV
Sbjct: 111 YLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAV 170

Query: 162 EGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGT 221
           EGIN+I  G L SLSEQEL+DCD T+ N GCNGG M  AF++I  T GL  E +YPY   
Sbjct: 171 EGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLME 230

Query: 222 ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSG 281
           E  C EQKE  + V+ISGYE VP ND++SL  A+A+QPVSVAI+A G +FQFY GG+F+G
Sbjct: 231 EGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNG 290

Query: 282 NCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMAS 340
            CG  L+HGVA VGYG +    Y +VKNSWG  WGE G+IRMKR++   +G CGI  MAS
Sbjct: 291 KCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMAS 350

BLAST of CSPI02G14740 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 9.8e-90
Identity = 169/308 (54.87%), Postives = 212/308 (68.83%), Query Frame = 1

Query: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101
           Y++W   +    +S  E ++RF +++ N  ++ N N M+  + L  N FAD+TN EF+ T
Sbjct: 38  YERWRSHHTVS-RSLHEKQKRFNVFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNT 97

Query: 102 YLGYKTVSIP--------DTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 161
           Y G K             +  F Y  +  +P +VDWR++GAVT +K+QGQCGSCWAFS +
Sbjct: 98  YSGSKVKHHRMFRGGPRGNGTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTI 157

Query: 162 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTTEIEYPY 221
            AVEGIN+IK  KL+SLSEQELVDCD T  NQGCNGG M  AFEFIK R G+TTE  YPY
Sbjct: 158 VAVEGINQIKTNKLVSLSEQELVDCD-TDQNQGCNGGLMDYAFEFIKQRGGITTEANYPY 217

Query: 222 QGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 281
           +  +  C+  KE    VSI G+E VP NDE +L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 218 EAYDGTCDVSKENAPAVSIDGHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGV 277

Query: 282 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 340
           F+G+CG +L+HGVAIVGYG T     YW VKNSWG +WGE GYIRM+R  +DK+G CGIA
Sbjct: 278 FTGSCGTELDHGVAIVGYGTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIA 337

BLAST of CSPI02G14740 vs. TrEMBL
Match: A0A0A0LJV6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 9.0e-199
Identity = 338/340 (99.41%), Postives = 339/340 (99.71%), Query Frame = 1

Query: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60
           MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE
Sbjct: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60

Query: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120
           RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM
Sbjct: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120

Query: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180
           VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV
Sbjct: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180

Query: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVN 240
           TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQG ESACNEQKEKYQFVSISGYEKVPVN
Sbjct: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVN 240

Query: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300
           DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL
Sbjct: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300

Query: 301 VKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 341
           VKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 301 VKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

BLAST of CSPI02G14740 vs. TrEMBL
Match: M5X1M2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.1e-115
Identity = 205/339 (60.47%), Postives = 252/339 (74.34%), Query Frame = 1

Query: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60
           M     SL FL++W+F      + +  Y    +    +++RY++W+ KYGR YK+REE  
Sbjct: 5   MVLTRASLTFLMVWIFCISS-TACSETYKPLRTDPKAMKERYERWLQKYGRIYKNREEAA 64

Query: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120
            RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T++G++T S P T F Y   
Sbjct: 65  YRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKTFMGFQTRSHPKTKFSYDKD 124

Query: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180
             LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+IK GKL+SLSEQELVDCDV
Sbjct: 125 EELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQIKTGKLVSLSEQELVDCDV 184

Query: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVN 240
            +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E   K   V+ISGYE +P N
Sbjct: 185 KTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDEDSLKNSAVNISGYESIPAN 244

Query: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300
            EKSL+AAVA+QPVSVA+DA G  FQFYS G F+G CG  LNHGV  VGYGE S + YW+
Sbjct: 245 SEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNLNHGVTAVGYGEDSGKKYWI 304

Query: 301 VKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 340
           VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 305 VKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

BLAST of CSPI02G14740 vs. TrEMBL
Match: G7ZUL7_MEDTR (Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 7.8e-110
Identity = 198/341 (58.06%), Postives = 251/341 (73.61%), Query Frame = 1

Query: 2   TWINVSLIFLILWVFWT--PRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEW 61
           T I +S++ L LW+  +  P +      ++  S+  + ++ RY+ W+ +YGR Y+ REEW
Sbjct: 3   TTITLSIVILNLWIIASACPEI------HTKNSTNPAVMKKRYETWLKRYGRHYRDREEW 62

Query: 62  ERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGN 121
           E RF IYQ+NVQYI+ +NS N+S+ L +N FAD+TNEEFK+TYLGY       T FRY  
Sbjct: 63  EVRFDIYQSNVQYIEFYNSQNYSYKLIDNRFADITNEEFKSTYLGYLPRFRVQTEFRYHK 122

Query: 122 MVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCD 181
              LP ++DWR++GAVT +K+QG+CGSCWAFSAVAAVEGINKIK   L+SLSEQ+L+DCD
Sbjct: 123 HGELPKSIDWRKKGAVTHVKDQGRCGSCWAFSAVAAVEGINKIKTENLVSLSEQQLIDCD 182

Query: 182 VTSGNQGCNGGYMYKAFEFIKR-TGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVP 241
           + SGN+GC GG MY AF +IK+  G+ T  EYPY+G +  CN+ K K   V+ISGYE VP
Sbjct: 183 IKSGNEGCEGGDMYIAFNYIKKHGGIATAKEYPYKGRDGNCNKSKAKNNAVTISGYESVP 242

Query: 242 VNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAY 301
             +EK LKAAVA+QPVS+A DA G  FQFYS GIFSG+CG  LNHG+ IVGYGE +   Y
Sbjct: 243 ARNEKMLKAAVAHQPVSIATDAGGYAFQFYSKGIFSGSCGKNLNHGMTIVGYGEENGDKY 302

Query: 302 WLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 340
           W+VKNSW  DWGESGY+RMKRD+ DK GTCGIAM A+YP K
Sbjct: 303 WIVKNSWANDWGESGYVRMKRDTKDKDGTCGIAMDATYPVK 337

BLAST of CSPI02G14740 vs. TrEMBL
Match: W9RAD3_9ROSA (KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.0e-109
Identity = 188/303 (62.05%), Postives = 232/303 (76.57%), Query Frame = 1

Query: 38  IQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEE 97
           ++ RY +W ++YGR Y S EE E RF IY  N+ +I+  NS N S+ L +N FAD+ N E
Sbjct: 44  VRQRYDRWAEQYGRNYGSEEEKELRFQIYHMNLLFIEQVNSQNFSYKLTDNKFADMMNAE 103

Query: 98  FKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVE 157
           F+   LGY+ +    T FR+G  + +P  VDWR+ GAVTP+K+QGQCGSCWAFS+VAAVE
Sbjct: 104 FRLRLLGYRPLLHNQTSFRFGGPMLVPKQVDWRKNGAVTPVKDQGQCGSCWAFSSVAAVE 163

Query: 158 GINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTES 217
           G+N+IK G+L+SLSEQELVDCDV +GNQGCNGGYM KAF+FIKR G+TT  +YPY+G   
Sbjct: 164 GVNQIKTGELVSLSEQELVDCDVNTGNQGCNGGYMEKAFQFIKRNGITTNGKYPYRGANG 223

Query: 218 ACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNC 277
            C+E K + + V ISGYEKVP NDE+ L+A VA+QPVSVAIDA G+ FQFYS GIF+G C
Sbjct: 224 RCDEDKLRGRRVKISGYEKVPHNDEERLQATVAHQPVSVAIDAGGSEFQFYSHGIFNGRC 283

Query: 278 GNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYP 337
           G  LNHGV +VGYGE   + YWLVKNSWGT+WGESGY+R+ R S D +GTCGIAM ASYP
Sbjct: 284 GTDLNHGVTVVGYGEEDGKTYWLVKNSWGTEWGESGYVRIHRGSVDGRGTCGIAMEASYP 343

Query: 338 TKD 341
            KD
Sbjct: 344 VKD 346

BLAST of CSPI02G14740 vs. TrEMBL
Match: A0A0D2W0V5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_012G047700 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.1e-107
Identity = 185/308 (60.06%), Postives = 238/308 (77.27%), Query Frame = 1

Query: 37  DIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNE 96
           D+Q+RYQ+W+ ++GR+YKS+ EW  RF IY++N Q+ID  NS N S  L +N FAD+TN+
Sbjct: 34  DMQERYQRWVARHGRKYKSKNEWALRFGIYKSNSQFIDCVNSQNLSFKLTDNEFADMTND 93

Query: 97  EFKATYLGYKTVSIP----DTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSA 156
           EF+A YLGY+++  P       F Y    NLP ++DWR++GAV PIKNQGQCGSCWAFSA
Sbjct: 94  EFRAMYLGYQSIRSPCESNSKGFAYDKYHNLPKSIDWRKKGAVAPIKNQGQCGSCWAFSA 153

Query: 157 VAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFI-KRTGLTTEIEYP 216
           VAA+EGIN+IK G L SLSEQEL+DCD  S +QGCNGG+M +A+EFI K  G+TTE +YP
Sbjct: 154 VAAIEGINQIKTGNLTSLSEQELIDCDTDSIDQGCNGGHMVQAYEFIIKNGGITTEKDYP 213

Query: 217 YQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGG 276
           Y G +  C   + K   V+ISGY+++P N+E +L+ AV+ QPVSVAIDA G  FQFY GG
Sbjct: 214 YTGRDDTCKRTQAKNHAVTISGYKRLPTNNETALQIAVSQQPVSVAIDAAGLEFQFYFGG 273

Query: 277 IFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 336
           +F+G+CGN+LNHGVAIVGYGE  N+ YW+VKNSWGT+WGE+GY+RM+R  +DK+G CGIA
Sbjct: 274 VFTGDCGNELNHGVAIVGYGEVLNKKYWIVKNSWGTEWGEAGYVRMERGVSDKRGLCGIA 333

Query: 337 MMASYPTK 340
           M  SYP K
Sbjct: 334 MDTSYPVK 341

BLAST of CSPI02G14740 vs. TAIR10
Match: AT1G06260.1 (AT1G06260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 348.2 bits (892), Expect = 5.7e-96
Identity = 182/340 (53.53%), Postives = 231/340 (67.94%), Query Frame = 1

Query: 5   NVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFT 64
           N++L  LI +V    +L S+  D S+     + ++ R++KW+  + + Y  R+EW  RF 
Sbjct: 9   NLTLAVLICFVLIASKLCSV--DSSVYDPHKT-LKQRFEKWLKTHSKLYGGRDEWMLRFG 68

Query: 65  IYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIP------DTCFRYG 124
           IYQ+NVQ ID  NS++    L +N FAD+TN EFKA +LG  T S+         C   G
Sbjct: 69  IYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAG 128

Query: 125 NMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDC 184
           N+   P  VDWR +GAVTPI+NQG+CG CWAFSAVAA+EGINKIK G L+SLSEQ+L+DC
Sbjct: 129 NV---PDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDC 188

Query: 185 DVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYPYQGTESACNEQKEKYQFVSISGYEKV 244
           DV + N+GC+GG M  AFEFIK  G L TE +YPY G E  C+++K K + V+I GY+KV
Sbjct: 189 DVGTYNKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTCDQEKSKNKVVTIQGYQKV 248

Query: 245 PVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQA 304
             N E SL+ A A QPVSV IDA G  FQ YS G+F+  CG  LNHGV +VGYG   +Q 
Sbjct: 249 AQN-EASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQK 308

Query: 305 YWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYP 338
           YW+VKNSWGT WGE GYIRM+R  ++  G CGIAMMASYP
Sbjct: 309 YWIVKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMASYP 341

BLAST of CSPI02G14740 vs. TAIR10
Match: AT5G45890.1 (AT5G45890.1 senescence-associated gene 12)

HSP 1 Score: 336.7 bits (862), Expect = 1.7e-92
Identity = 172/314 (54.78%), Postives = 224/314 (71.34%), Query Frame = 1

Query: 38  IQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHT--LAENNFADLTN 97
           +Q R+ +WM K+GR Y   +E   R+ +++ NV+ I++ NS+    T  LA N FADLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 98  EEFKATYLGYKTVSIPDTC-------FRYGNMVN--LPTNVDWRQEGAVTPIKNQGQCGS 157
           +EF++ Y G+K VS   +        FRY N+ +  LP +VDWR++GAVTPIKNQG CG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGC 153

Query: 158 CWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LT 217
           CWAFSAVAA+EG  +IK GKLISLSEQ+LVDCD  + + GC GG M  AFE IK TG LT
Sbjct: 154 CWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLT 213

Query: 218 TEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNF 277
           TE  YPY+G ++ CN +K   +  SI+GYE VPVNDE++L  AVA+QPVSV I+  G +F
Sbjct: 214 TESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDF 273

Query: 278 QFYSGGIFSGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDK 337
           QFYS G+F+G C   L+H V  +GYGE++N   YW++KNSWGT WGESGY+R+++D  DK
Sbjct: 274 QFYSSGVFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDK 333

Query: 338 QGTCGIAMMASYPT 339
           QG CG+AM ASYPT
Sbjct: 334 QGLCGLAMKASYPT 345

BLAST of CSPI02G14740 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 335.1 bits (858), Expect = 5.0e-92
Identity = 171/315 (54.29%), Postives = 217/315 (68.89%), Query Frame = 1

Query: 36  SDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTN 95
           + + + Y++W   +    +S EE  +RF +++ NV++I   N  + S+ L  N F D+T+
Sbjct: 32  NSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTS 91

Query: 96  EEFKATYLG--------YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSC 155
           EEF+ TY G        ++        F Y N+  LPT+VDWR+ GAVTP+KNQGQCGSC
Sbjct: 92  EEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSC 151

Query: 156 WAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTT 215
           WAFS V AVEGIN+I+  KL SLSEQELVDCD T+ NQGCNGG M  AFEFIK + GLT+
Sbjct: 152 WAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTS 211

Query: 216 EIEYPYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQ 275
           E+ YPY+ ++  C+  KE    VSI G+E VP N E  L  AVANQPVSVAIDA G++FQ
Sbjct: 212 ELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQ 271

Query: 276 FYSGGIFSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQ 335
           FYS G+F+G CG +LNHGVA+VGYG T     YW+VKNSWG +WGE GYIRM+R    K+
Sbjct: 272 FYSEGVFTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKE 331

Query: 336 GTCGIAMMASYPTKD 341
           G CGIAM ASYP K+
Sbjct: 332 GLCGIAMEASYPLKN 344

BLAST of CSPI02G14740 vs. TAIR10
Match: AT4G35350.1 (AT4G35350.1 xylem cysteine peptidase 1)

HSP 1 Score: 334.7 bits (857), Expect = 6.6e-92
Identity = 168/304 (55.26%), Postives = 214/304 (70.39%), Query Frame = 1

Query: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101
           ++ WM ++ + YKS EE   RF +++ N+ +ID  N+  +S+ L  N FADLT+EEFK  
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGR 110

Query: 102 YLG-----YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAV 161
           YLG     +     P   FRY ++ +LP +VDWR++GAV P+K+QGQCGSCWAFS VAAV
Sbjct: 111 YLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAV 170

Query: 162 EGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGT 221
           EGIN+I  G L SLSEQEL+DCD T+ N GCNGG M  AF++I  T GL  E +YPY   
Sbjct: 171 EGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLME 230

Query: 222 ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSG 281
           E  C EQKE  + V+ISGYE VP ND++SL  A+A+QPVSVAI+A G +FQFY GG+F+G
Sbjct: 231 EGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNG 290

Query: 282 NCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMAS 340
            CG  L+HGVA VGYG +    Y +VKNSWG  WGE G+IRMKR++   +G CGI  MAS
Sbjct: 291 KCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMAS 350

BLAST of CSPI02G14740 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 330.9 bits (847), Expect = 9.5e-91
Identity = 170/332 (51.20%), Postives = 221/332 (66.57%), Query Frame = 1

Query: 30  LGSSCSSDIQDR-----------YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNS 89
           L ++C  D  D+           Y +W   +    +S  E E+RF +++ NV ++ N N 
Sbjct: 15  LQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNK 74

Query: 90  MNHSHTLAENNFADLTNEEFKATYLG-----YKTVSIPDT-----CFRYGNMVNLPTNVD 149
            N S+ L  N FADLT  EFK  Y G     ++ +  P        + + N+  LP++VD
Sbjct: 75  KNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVD 134

Query: 150 WRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCN 209
           WR++GAVT IKNQG+CGSCWAFS VAAVEGINKIK  KL+SLSEQELVDCD T  N+GCN
Sbjct: 135 WRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCN 194

Query: 210 GGYMYKAFEFIKRTG-LTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKA 269
           GG M  AFEFIK+ G +TTE  YPY+G +  C+  K+    V+I G+E VP NDE +L  
Sbjct: 195 GGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLK 254

Query: 270 AVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGT 329
           AVANQPVSVAIDA  ++FQFYS G+F+G+CG +LNHGVA VGYG    + YW+V+NSWG 
Sbjct: 255 AVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGA 314

Query: 330 DWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 340
           +WGE GYI+++R+  + +G CGIAM ASYP K
Sbjct: 315 EWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI02G14740 vs. NCBI nr
Match: gi|700206934|gb|KGN62053.1| (hypothetical protein Csa_2G292830 [Cucumis sativus])

HSP 1 Score: 700.7 bits (1807), Expect = 1.3e-198
Identity = 338/340 (99.41%), Postives = 339/340 (99.71%), Query Frame = 1

Query: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60
           MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE
Sbjct: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60

Query: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120
           RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM
Sbjct: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120

Query: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180
           VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV
Sbjct: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180

Query: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVN 240
           TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQG ESACNEQKEKYQFVSISGYEKVPVN
Sbjct: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVN 240

Query: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300
           DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL
Sbjct: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300

Query: 301 VKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 341
           VKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 301 VKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

BLAST of CSPI02G14740 vs. NCBI nr
Match: gi|449460678|ref|XP_004148072.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 656.0 bits (1691), Expect = 3.6e-185
Identity = 315/317 (99.37%), Postives = 316/317 (99.68%), Query Frame = 1

Query: 24  MAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSH 83
           MAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSH
Sbjct: 1   MAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSH 60

Query: 84  TLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQ 143
           TLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQ
Sbjct: 61  TLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQ 120

Query: 144 CGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG 203
           CGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG
Sbjct: 121 CGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG 180

Query: 204 LTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGN 263
           LTTEIEYPYQG ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGN
Sbjct: 181 LTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGN 240

Query: 264 NFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD 323
           NFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD
Sbjct: 241 NFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD 300

Query: 324 KQGTCGIAMMASYPTKD 341
           +QGTCGIAMMASYPTKD
Sbjct: 301 RQGTCGIAMMASYPTKD 317

BLAST of CSPI02G14740 vs. NCBI nr
Match: gi|659117224|ref|XP_008458487.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 615.5 bits (1586), Expect = 5.5e-173
Identity = 301/342 (88.01%), Postives = 317/342 (92.69%), Query Frame = 1

Query: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60
           M W +VSLI LILWVFWTP  VSMAMDY  GSS S ++QDRYQKWMDKYGRQYKSREEWE
Sbjct: 6   MIW-SVSLISLILWVFWTPTRVSMAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWE 65

Query: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDT--CFRYG 120
           +RFTIYQANVQYIDNFNS+NHS+TLAENNF DLTNEEF ATYLGY+TVS+PDT   FRYG
Sbjct: 66  QRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYG 125

Query: 121 NMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDC 180
           NMVNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKL+SLSEQELVDC
Sbjct: 126 NMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDC 185

Query: 181 DVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVP 240
           DVTSGNQGCNGGYMYKAFEFIK+TGLTTE+EYPY  T SAC++QKEKYQ VSISGYEKVP
Sbjct: 186 DVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVP 245

Query: 241 VNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAY 300
           VNDEKSL+AAVA QPVSVAIDA GN+FQFYSGGIFSGNCG QLNHGVAIVGYGE SNQAY
Sbjct: 246 VNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQAY 305

Query: 301 WLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 341
           WLVKNSWGT WGESGYIRM RDSTDKQGTCGIAMMASYP KD
Sbjct: 306 WLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIKD 346

BLAST of CSPI02G14740 vs. NCBI nr
Match: gi|645245974|ref|XP_008229136.1| (PREDICTED: zingipain-2 [Prunus mume])

HSP 1 Score: 425.2 bits (1092), Expect = 1.0e-115
Identity = 205/339 (60.47%), Postives = 253/339 (74.63%), Query Frame = 1

Query: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60
           M     SL F ++W+F      + +  Y    +    +++RY++W+ KYGR YK+REE E
Sbjct: 5   MVLTRASLTFFMVWIFCISS-TACSETYKPLRTDPKAMKERYERWLQKYGRIYKNREEAE 64

Query: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120
            RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T++G++T S P T F Y   
Sbjct: 65  YRFGVYKSNIEFVDFVNSQNQSYKLTDNKFADITNLEFTNTFMGFQTRSHPKTKFSYDKD 124

Query: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180
            +LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+IK GKL+SLSEQELVDCDV
Sbjct: 125 EDLPTAVDWRKNGAVTPIKNQGQCGSCWAFSAVAAVEGINQIKTGKLVSLSEQELVDCDV 184

Query: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVN 240
            +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E   K   V+ISGYE +P N
Sbjct: 185 KTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDEDSLKNHAVNISGYESIPAN 244

Query: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300
            EKSL+AAVA+QPVSVA+DA    FQFYS GIF+G CG  LNHGV  VGYGE S + YW+
Sbjct: 245 SEKSLQAAVAHQPVSVAVDAASYAFQFYSSGIFTGQCGKNLNHGVTAVGYGEDSGKKYWI 304

Query: 301 VKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 340
           VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 305 VKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

BLAST of CSPI02G14740 vs. NCBI nr
Match: gi|595908837|ref|XP_007214244.1| (hypothetical protein PRUPE_ppa023515mg [Prunus persica])

HSP 1 Score: 423.7 bits (1088), Expect = 3.0e-115
Identity = 205/339 (60.47%), Postives = 252/339 (74.34%), Query Frame = 1

Query: 1   MTWINVSLIFLILWVFWTPRLVSMAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWE 60
           M     SL FL++W+F      + +  Y    +    +++RY++W+ KYGR YK+REE  
Sbjct: 5   MVLTRASLTFLMVWIFCISS-TACSETYKPLRTDPKAMKERYERWLQKYGRIYKNREEAA 64

Query: 61  RRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNM 120
            RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T++G++T S P T F Y   
Sbjct: 65  YRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKTFMGFQTRSHPKTKFSYDKD 124

Query: 121 VNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDV 180
             LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+IK GKL+SLSEQELVDCDV
Sbjct: 125 EELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQIKTGKLVSLSEQELVDCDV 184

Query: 181 TSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGTESACNEQKEKYQFVSISGYEKVPVN 240
            +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E   K   V+ISGYE +P N
Sbjct: 185 KTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDEDSLKNSAVNISGYESIPAN 244

Query: 241 DEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWL 300
            EKSL+AAVA+QPVSVA+DA G  FQFYS G F+G CG  LNHGV  VGYGE S + YW+
Sbjct: 245 SEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNLNHGVTAVGYGEDSGKKYWI 304

Query: 301 VKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 340
           VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 305 VKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SAG12_ARATH3.1e-9154.78Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana GN=SAG12 PE=... [more]
CYSEP_VIGMU5.2e-9154.98Vignain OS=Vigna mungo PE=1 SV=1[more]
CEP1_ARATH8.9e-9154.29KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=... [more]
XCP1_ARATH1.2e-9055.26Cysteine protease XCP1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1[more]
CYSEP_RICCO9.8e-9054.87Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LJV6_CUCSA9.0e-19999.41Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1[more]
M5X1M2_PRUPE2.1e-11560.47Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1[more]
G7ZUL7_MEDTR7.8e-11058.06Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1[more]
W9RAD3_9ROSA1.0e-10962.05KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 S... [more]
A0A0D2W0V5_GOSRA2.1e-10760.06Uncharacterized protein OS=Gossypium raimondii GN=B456_012G047700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G06260.15.7e-9653.53 Cysteine proteinases superfamily protein[more]
AT5G45890.11.7e-9254.78 senescence-associated gene 12[more]
AT5G50260.15.0e-9254.29 Cysteine proteinases superfamily protein[more]
AT4G35350.16.6e-9255.26 xylem cysteine peptidase 1[more]
AT3G48340.19.5e-9151.20 Cysteine proteinases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700206934|gb|KGN62053.1|1.3e-19899.41hypothetical protein Csa_2G292830 [Cucumis sativus][more]
gi|449460678|ref|XP_004148072.1|3.6e-18599.37PREDICTED: ervatamin-B-like [Cucumis sativus][more]
gi|659117224|ref|XP_008458487.1|5.5e-17388.01PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|645245974|ref|XP_008229136.1|1.0e-11560.47PREDICTED: zingipain-2 [Prunus mume][more]
gi|595908837|ref|XP_007214244.1|3.0e-11560.47hypothetical protein PRUPE_ppa023515mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000169Pept_cys_AS
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
IPR025660Pept_his_AS
IPR025661Pept_asp_AS
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G14740.1CSPI02G14740.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 141..152
scor
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 141..156
score: 1.1E-10coord: 298..304
score: 1.1E-10coord: 283..293
score: 1.1
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 123..338
score: 3.3
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 123..338
score: 6.8E
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 8..337
score: 3.8E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 42..98
score: 1.3
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 42..98
score: 4.4
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 281..291
scor
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 298..317
scor
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 16..338
score: 6.7E
NoneNo IPR availablePANTHERPTHR12411:SF382CYSTEINE PROTEINASES SUPERFAMILY PROTEINcoord: 8..337
score: 3.8E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 39..338
score: 2.1E