Cucsa.366410.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.366410.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Cysteine proteinase
Location	scaffold03625 : 44764 .. 45849 (-)
Sequence length	901

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGTATGAACTTGTAATACTCTCATTCATTCAAATTATCAGAAATACGTTGAACATGTTCTTTTTACGTCTGATTATAAGTTTTTTTTTTCCTATTCCTAACAAGTACCCTAAACCCTACCTTCTATCCATTGTAAGTCATTTCATCGTATTTGGAAAAACTTACCAATCTTGAATTTTCGACACAGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAGCAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

mRNA sequence

GTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAGCAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

Coding sequence (CDS)

GTACCAGAAATGGATGGATAAATACGGTCGACAATACAAGAGCAGAGAAGAATGGGAGCGGAGATTCACAATTTATCAAGCGAATGTTCAATACATTGACAATTTCAATTCTATGAATCATTCACATACTCTGGCTGAAAATAACTTTGCAGATCTCACAAATGAGGAGTTTAAGGCAACTTACTTAGGGTATAAAACCGTTTCGATTCCTGATACATGCTTCAGATATGGAAATATGGTTAATCTGCCTACTAATGTTGATTGGAGACAGGAAGGTGCAGTTACTCCGATAAAGAATCAAGGCCAATGTGGGAGCTGTTGGGCATTCTCTGCAGTAGCAGCAGTCGAAGGCATCAACAAAATAAAAGCAGGAAAATTGATATCTCTTTCAGAACAAGAGCTTGTAGACTGCGATGTGACCTCGGGGAACCAGGGATGTAATGGTGGTTACATGTACAAAGCATTTGAGTTCATCAAGAGAACTGGACTAACTACAGAAATAGAATATCCATACCAAGGAGCAGAATCTGCATGCAACGAACAAAAAGAGAAATACCAATTTGTGTCAATAAGTGGATATGAAAAAGTACCGGTCAACGACGAGAAAAGCTTAAAAGCAGCAGTTGCTAACCAGCCAGTCTCTGTAGCAATTGATGCAGAGGGAAACAATTTTCAGTTCTATTCTGGTGGAATCTTCTCAGGCAATTGTGGAAACCAACTGAATCATGGAGTGGCAATAGTTGGGTATGGGGAAACTAGCAATCAAGCTTATTGGCTTGTCAAGAATTCATGGGGCACTGATTGGGGTGAATCAGGTTACATAAGAATGAAGCGTGATTCAACTGACAAGCAAGGTACTTGTGGCATAGCTATGATGGCTAGCTACCCCACCAAAGACTGA

Protein sequence

YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD*

Homology

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BLAST of Cucsa.366410.1 vs. Swiss-Prot
Match: CEP1_ARATH (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 171/309 (55.34%), Postives = 214/309 (69.26%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W   +    +S EE  +RF +++ NV++I   N  + S+ L  N F D+T+EEF+ T
Sbjct: 38  YERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRT 97

Query: 61  YLG--------YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 120
           Y G        ++        F Y N+  LPT+VDWR+ GAVTP+KNQGQCGSCWAFS V
Sbjct: 98  YAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTV 157

Query: 121 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTTEIEYPY 180
            AVEGIN+I+  KL SLSEQELVDCD T+ NQGCNGG M  AFEFIK + GLT+E+ YPY
Sbjct: 158 VAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPY 217

Query: 181 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 240
           + ++  C+  KE    VSI G+E VP N E  L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 218 KASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGV 277

Query: 241 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 300
           F+G CG +LNHGVA+VGYG T     YW+VKNSWG +WGE GYIRM+R    K+G CGIA
Sbjct: 278 FTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIA 337

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BLAST of Cucsa.366410.1 vs. Swiss-Prot
Match: XCP1_ARATH (Cysteine protease XCP1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.4e-92
Identity = 168/304 (55.26%), Postives = 214/304 (70.39%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           ++ WM ++ + YKS EE   RF +++ N+ +ID  N+  +S+ L  N FADLT+EEFK  
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGR 110

Query: 61  YLG-----YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAV 120
           YLG     +     P   FRY ++ +LP +VDWR++GAV P+K+QGQCGSCWAFS VAAV
Sbjct: 111 YLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAV 170

Query: 121 EGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGA 180
           EGIN+I  G L SLSEQEL+DCD T+ N GCNGG M  AF++I  T GL  E +YPY   
Sbjct: 171 EGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLME 230

Query: 181 ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSG 240
           E  C EQKE  + V+ISGYE VP ND++SL  A+A+QPVSVAI+A G +FQFY GG+F+G
Sbjct: 231 EGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNG 290

Query: 241 NCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMAS 299
            CG  L+HGVA VGYG +    Y +VKNSWG  WGE G+IRMKR++   +G CGI  MAS
Sbjct: 291 KCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMAS 350

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BLAST of Cucsa.366410.1 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.2e-92
Identity = 170/309 (55.02%), Postives = 214/309 (69.26%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W   +    +S  E  +RF +++ANV ++ N N M+  + L  N FAD+TN EF++T
Sbjct: 40  YERWRSHHTVS-RSLGEKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRST 99

Query: 61  YLGYKT--------VSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 120
           Y G K                F Y  + ++P +VDWR++GAVT +K+QGQCGSCWAFS +
Sbjct: 100 YAGSKVNHHKMFRGSQHGSGTFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTI 159

Query: 121 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYPY 180
            AVEGIN+IK  KL+SLSEQELVDCD    NQGCNGG M  AFEFIK+ G +TTE  YPY
Sbjct: 160 VAVEGINQIKTNKLVSLSEQELVDCDKEE-NQGCNGGLMESAFEFIKQKGGITTESNYPY 219

Query: 181 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 240
              E  C+E K     VSI G+E VPVNDE +L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 220 TAQEGTCDESKVNDLAVSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGV 279

Query: 241 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 300
           F+G+C   LNHGVAIVGYG T     YW+V+NSWG +WGE GYIRM+R+ + K+G CGIA
Sbjct: 280 FTGDCNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIA 339

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BLAST of Cucsa.366410.1 vs. Swiss-Prot
Match: SAG12_ARATH (Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana GN=SAG12 PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.4e-92
Identity = 170/308 (55.19%), Postives = 220/308 (71.43%), Query Frame = 1


Query: 3   KWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHT--LAENNFADLTNEEFKAT 62
           +WM K+GR Y   +E   R+ +++ NV+ I++ NS+    T  LA N FADLTN+EF++ 
Sbjct: 40  EWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSM 99

Query: 63  YLGYKTVSIPDTC-------FRYGNMVN--LPTNVDWRQEGAVTPIKNQGQCGSCWAFSA 122
           Y G+K VS   +        FRY N+ +  LP +VDWR++GAVTPIKNQG CG CWAFSA
Sbjct: 100 YTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSA 159

Query: 123 VAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYP 182
           VAA+EG  +IK GKLISLSEQ+LVDCD  + + GC GG M  AFE IK TG LTTE  YP
Sbjct: 160 VAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLTTESNYP 219

Query: 183 YQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGG 242
           Y+G ++ CN +K   +  SI+GYE VPVNDE++L  AVA+QPVSV I+  G +FQFYS G
Sbjct: 220 YKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSG 279

Query: 243 IFSGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGI 298
           +F+G C   L+H V  +GYGE++N   YW++KNSWGT WGESGY+R+++D  DKQG CG+
Sbjct: 280 VFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGL 339

[Close]

BLAST of Cucsa.366410.1 vs. Swiss-Prot
Match: SAG39_ORYSI (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica GN=OsI_14861 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.1e-91
Identity = 171/306 (55.88%), Postives = 215/306 (70.26%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFK-- 60
           +++WM +YGR Y+   E  RRF +++ANV +I++FN+ NH+  L  N FADLTN+EF+  
Sbjct: 37  HERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFADLTNDEFRWT 96

Query: 61  ATYLGY--KTVSIPDTCFRYGNMVN---LPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVA 120
            T  G+   T  +P T FRY N VN   LP  VDWR +GAVTPIK+QGQCG CWAFSAVA
Sbjct: 97  KTNKGFIPSTTRVP-TGFRYEN-VNIDALPATVDWRTKGAVTPIKDQGQCGCCWAFSAVA 156

Query: 121 AVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEF-IKRTGLTTEIEYPYQ 180
           A+EGI K+  GKLISLSEQELVDCDV   +QGC GG M  AF+F IK  GLTTE  YPY 
Sbjct: 157 AMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLTTESNYPYA 216

Query: 181 GAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIF 240
            A+  C          SI GYE VP N+E +L  AVANQPVSVA+D     FQFY GG+ 
Sbjct: 217 AADDKCKSVSN--SVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDMTFQFYKGGVM 276

Query: 241 SGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAM 298
           +G+CG  L+HG+  +GYG+ S+   YWL+KNSWGT WGE+G++RM++D +DK+G CG+AM
Sbjct: 277 TGSCGTDLDHGIVAIGYGKASDGTKYWLLKNSWGTTWGENGFLRMEKDISDKRGMCGLAM 336

[Close]

BLAST of Cucsa.366410.1 vs. TrEMBL
Match: A0A0A0LJV6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 8.5e-177
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT
Sbjct: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK
Sbjct: 102 YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 161

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE
Sbjct: 162 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 221

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
           QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL
Sbjct: 222 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 281

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 282 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]

BLAST of Cucsa.366410.1 vs. TrEMBL
Match: M5X1M2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.2e-115
Identity = 196/298 (65.77%), Postives = 234/298 (78.52%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W+ KYGR YK+REE   RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T
Sbjct: 45  YERWLQKYGRIYKNREEAAYRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKT 104

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           ++G++T S P T F Y     LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+
Sbjct: 105 FMGFQTRSHPKTKFSYDKDEELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQ 164

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK GKL+SLSEQELVDCDV +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E
Sbjct: 165 IKTGKLVSLSEQELVDCDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDE 224

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
              K   V+ISGYE +P N EKSL+AAVA+QPVSVA+DA G  FQFYS G F+G CG  L
Sbjct: 225 DSLKNSAVNISGYESIPANSEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNL 284

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           NHGV  VGYGE S + YW+VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 285 NHGVTAVGYGEDSGKKYWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

[Close]

BLAST of Cucsa.366410.1 vs. TrEMBL
Match: W9RAD3_9ROSA (KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 8.1e-111
Identity = 188/299 (62.88%), Postives = 230/299 (76.92%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y +W ++YGR Y S EE E RF IY  N+ +I+  NS N S+ L +N FAD+ N EF+  
Sbjct: 48  YDRWAEQYGRNYGSEEEKELRFQIYHMNLLFIEQVNSQNFSYKLTDNKFADMMNAEFRLR 107

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
            LGY+ +    T FR+G  + +P  VDWR+ GAVTP+K+QGQCGSCWAFS+VAAVEG+N+
Sbjct: 108 LLGYRPLLHNQTSFRFGGPMLVPKQVDWRKNGAVTPVKDQGQCGSCWAFSSVAAVEGVNQ 167

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK G+L+SLSEQELVDCDV +GNQGCNGGYM KAF+FIKR G+TT  +YPY+GA   C+E
Sbjct: 168 IKTGELVSLSEQELVDCDVNTGNQGCNGGYMEKAFQFIKRNGITTNGKYPYRGANGRCDE 227

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
            K + + V ISGYEKVP NDE+ L+A VA+QPVSVAIDA G+ FQFYS GIF+G CG  L
Sbjct: 228 DKLRGRRVKISGYEKVPHNDEERLQATVAHQPVSVAIDAGGSEFQFYSHGIFNGRCGTDL 287

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGV +VGYGE   + YWLVKNSWGT+WGESGY+R+ R S D +GTCGIAM ASYP KD
Sbjct: 288 NHGVTVVGYGEEDGKTYWLVKNSWGTEWGESGYVRIHRGSVDGRGTCGIAMEASYPVKD 346

[Close]

BLAST of Cucsa.366410.1 vs. TrEMBL
Match: G7ZUL7_MEDTR (Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.4e-110
Identity = 189/299 (63.21%), Postives = 230/299 (76.92%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y+ W+ +YGR Y+ REEWE RF IYQ+NVQYI+ +NS N+S+ L +N FAD+TNEEFK+T
Sbjct: 39  YETWLKRYGRHYRDREEWEVRFDIYQSNVQYIEFYNSQNYSYKLIDNRFADITNEEFKST 98

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGY       T FRY     LP ++DWR++GAVT +K+QG+CGSCWAFSAVAAVEGINK
Sbjct: 99  YLGYLPRFRVQTEFRYHKHGELPKSIDWRKKGAVTHVKDQGRCGSCWAFSAVAAVEGINK 158

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKR-TGLTTEIEYPYQGAESACN 180
           IK   L+SLSEQ+L+DCD+ SGN+GC GG MY AF +IK+  G+ T  EYPY+G +  CN
Sbjct: 159 IKTENLVSLSEQQLIDCDIKSGNEGCEGGDMYIAFNYIKKHGGIATAKEYPYKGRDGNCN 218

Query: 181 EQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQ 240
           + K K   V+ISGYE VP  +EK LKAAVA+QPVS+A DA G  FQFYS GIFSG+CG  
Sbjct: 219 KSKAKNNAVTISGYESVPARNEKMLKAAVAHQPVSIATDAGGYAFQFYSKGIFSGSCGKN 278

Query: 241 LNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           LNHG+ IVGYGE +   YW+VKNSW  DWGESGY+RMKRD+ DK GTCGIAM A+YP K
Sbjct: 279 LNHGMTIVGYGEENGDKYWIVKNSWANDWGESGYVRMKRDTKDKDGTCGIAMDATYPVK 337

[Close]

BLAST of Cucsa.366410.1 vs. TrEMBL
Match: A0A151TPE0_CAJCA (Vignain OS=Cajanus cajan GN=KK1_022504 PE=3 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-107
Identity = 190/301 (63.12%), Postives = 231/301 (76.74%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           +++W+ ++GR YK +EEWE RF IYQAN+++I+  NS N+S+ L +N FADLTNEEF +T
Sbjct: 5   FERWITQHGRNYKDKEEWEVRFGIYQANLKFIECKNSQNNSYNLIDNKFADLTNEEFMST 64

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLG+ T     T        +LP ++DWR+EGAVT IK+QG CGSCWAFSAVAAVEGINK
Sbjct: 65  YLGFGTRLPTHTGIGSYKHGDLPESMDWRKEGAVTEIKDQGNCGSCWAFSAVAAVEGINK 124

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGAESACN 180
           IK+GKL+SLSEQEL+DCDV +GNQGC GG M  AF+FIKR  GL T  EYPY+G +  CN
Sbjct: 125 IKSGKLVSLSEQELIDCDVENGNQGCEGGLMDTAFKFIKRNGGLATAKEYPYEGVDGTCN 184

Query: 181 EQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQ 240
           ++K  +  V+ISGYE+VP N+E  LKAAVANQPVSVAIDA G  FQ YS G+FSG CG  
Sbjct: 185 KEKAGHHAVNISGYERVPSNNEAMLKAAVANQPVSVAIDAGGYEFQLYSEGVFSGRCGKH 244

Query: 241 LNHGVAIVGYGETS-NQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 300
           LNHGV +VGYGE +    YW+VKNSWG DWGESGY+RMKRD+ D  G CGIAM ASYP K
Sbjct: 245 LNHGVTVVGYGEENIGDKYWIVKNSWGLDWGESGYVRMKRDTVDDAGICGIAMQASYPLK 304

[Close]

BLAST of Cucsa.366410.1 vs. TAIR10
Match: AT1G06260.1 (AT1G06260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 350.5 bits (898), Expect = 1.0e-96
Identity = 172/303 (56.77%), Postives = 212/303 (69.97%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           ++KW+  + + Y  R+EW  RF IYQ+NVQ ID  NS++    L +N FAD+TN EFKA 
Sbjct: 43  FEKWLKTHSKLYGGRDEWMLRFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAH 102

Query: 61  YLGYKTVSIP------DTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAA 120
           +LG  T S+         C   GN+   P  VDWR +GAVTPI+NQG+CG CWAFSAVAA
Sbjct: 103 FLGLNTSSLRLHKKQRPVCDPAGNV---PDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAA 162

Query: 121 VEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYPYQG 180
           +EGINKIK G L+SLSEQ+L+DCDV + N+GC+GG M  AFEFIK  G L TE +YPY G
Sbjct: 163 IEGINKIKTGNLVSLSEQQLIDCDVGTYNKGCSGGLMETAFEFIKTNGGLATETDYPYTG 222

Query: 181 AESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFS 240
            E  C+++K K + V+I GY+KV  N E SL+ A A QPVSV IDA G  FQ YS G+F+
Sbjct: 223 IEGTCDQEKSKNKVVTIQGYQKVAQN-EASLQIAAAQQPVSVGIDAGGFIFQLYSSGVFT 282

Query: 241 GNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMA 297
             CG  LNHGV +VGYG   +Q YW+VKNSWGT WGE GYIRM+R  ++  G CGIAMMA
Sbjct: 283 NYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEEGYIRMERGVSEDTGKCGIAMMA 341

[Close]

BLAST of Cucsa.366410.1 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 340.5 bits (872), Expect = 1.1e-93
Identity = 171/309 (55.34%), Postives = 214/309 (69.26%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W   +    +S EE  +RF +++ NV++I   N  + S+ L  N F D+T+EEF+ T
Sbjct: 38  YERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRT 97

Query: 61  YLG--------YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAV 120
           Y G        ++        F Y N+  LPT+VDWR+ GAVTP+KNQGQCGSCWAFS V
Sbjct: 98  YAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTV 157

Query: 121 AAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK-RTGLTTEIEYPY 180
            AVEGIN+I+  KL SLSEQELVDCD T+ NQGCNGG M  AFEFIK + GLT+E+ YPY
Sbjct: 158 VAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPY 217

Query: 181 QGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGI 240
           + ++  C+  KE    VSI G+E VP N E  L  AVANQPVSVAIDA G++FQFYS G+
Sbjct: 218 KASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGV 277

Query: 241 FSGNCGNQLNHGVAIVGYGET-SNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIA 300
           F+G CG +LNHGVA+VGYG T     YW+VKNSWG +WGE GYIRM+R    K+G CGIA
Sbjct: 278 FTGRCGTELNHGVAVVGYGTTIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIA 337

[Close]

BLAST of Cucsa.366410.1 vs. TAIR10
Match: AT4G35350.1 (AT4G35350.1 xylem cysteine peptidase 1)

HSP 1 Score: 340.1 bits (871), Expect = 1.4e-93
Identity = 168/304 (55.26%), Postives = 214/304 (70.39%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           ++ WM ++ + YKS EE   RF +++ N+ +ID  N+  +S+ L  N FADLT+EEFK  
Sbjct: 51  FESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLGLNEFADLTHEEFKGR 110

Query: 61  YLG-----YKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAV 120
           YLG     +     P   FRY ++ +LP +VDWR++GAV P+K+QGQCGSCWAFS VAAV
Sbjct: 111 YLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAV 170

Query: 121 EGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRT-GLTTEIEYPYQGA 180
           EGIN+I  G L SLSEQEL+DCD T+ N GCNGG M  AF++I  T GL  E +YPY   
Sbjct: 171 EGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYAFQYIISTGGLHKEDDYPYLME 230

Query: 181 ESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSG 240
           E  C EQKE  + V+ISGYE VP ND++SL  A+A+QPVSVAI+A G +FQFY GG+F+G
Sbjct: 231 EGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVAIEASGRDFQFYKGGVFNG 290

Query: 241 NCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMAS 299
            CG  L+HGVA VGYG +    Y +VKNSWG  WGE G+IRMKR++   +G CGI  MAS
Sbjct: 291 KCGTDLDHGVAAVGYGSSKGSDYVIVKNSWGPRWGEKGFIRMKRNTGKPEGLCGINKMAS 350

[Close]

BLAST of Cucsa.366410.1 vs. TAIR10
Match: AT5G45890.1 (AT5G45890.1 senescence-associated gene 12)

HSP 1 Score: 339.0 bits (868), Expect = 3.1e-93
Identity = 170/308 (55.19%), Postives = 220/308 (71.43%), Query Frame = 1


Query: 3   KWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHT--LAENNFADLTNEEFKAT 62
           +WM K+GR Y   +E   R+ +++ NV+ I++ NS+    T  LA N FADLTN+EF++ 
Sbjct: 40  EWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSM 99

Query: 63  YLGYKTVSIPDTC-------FRYGNMVN--LPTNVDWRQEGAVTPIKNQGQCGSCWAFSA 122
           Y G+K VS   +        FRY N+ +  LP +VDWR++GAVTPIKNQG CG CWAFSA
Sbjct: 100 YTGFKGVSALSSQSQTKMSPFRYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSA 159

Query: 123 VAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEYP 182
           VAA+EG  +IK GKLISLSEQ+LVDCD  + + GC GG M  AFE IK TG LTTE  YP
Sbjct: 160 VAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATGGLTTESNYP 219

Query: 183 YQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGG 242
           Y+G ++ CN +K   +  SI+GYE VPVNDE++L  AVA+QPVSV I+  G +FQFYS G
Sbjct: 220 YKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSG 279

Query: 243 IFSGNCGNQLNHGVAIVGYGETSN-QAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGI 298
           +F+G C   L+H V  +GYGE++N   YW++KNSWGT WGESGY+R+++D  DKQG CG+
Sbjct: 280 VFTGECTTYLDHAVTAIGYGESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGL 339

[Close]

BLAST of Cucsa.366410.1 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 335.1 bits (858), Expect = 4.4e-92
Identity = 166/309 (53.72%), Postives = 214/309 (69.26%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y +W   +    +S  E E+RF +++ NV ++ N N  N S+ L  N FADLT  EFK  
Sbjct: 38  YDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNA 97

Query: 61  YLG-----YKTVSIPDT-----CFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFS 120
           Y G     ++ +  P        + + N+  LP++VDWR++GAVT IKNQG+CGSCWAFS
Sbjct: 98  YTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFS 157

Query: 121 AVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG-LTTEIEY 180
            VAAVEGINKIK  KL+SLSEQELVDCD T  N+GCNGG M  AFEFIK+ G +TTE  Y
Sbjct: 158 TVAAVEGINKIKTNKLVSLSEQELVDCD-TKQNEGCNGGLMEIAFEFIKKNGGITTEDSY 217

Query: 181 PYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSG 240
           PY+G +  C+  K+    V+I G+E VP NDE +L  AVANQPVSVAIDA  ++FQFYS 
Sbjct: 218 PYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE 277

Query: 241 GIFSGNCGNQLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGI 299
           G+F+G+CG +LNHGVA VGYG    + YW+V+NSWG +WGE GYI+++R+  + +G CGI
Sbjct: 278 GVFTGSCGTELNHGVAAVGYGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGI 337

[Close]

BLAST of Cucsa.366410.1 vs. NCBI nr
Match: gi|449460678|ref|XP_004148072.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-176
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT
Sbjct: 19  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 78

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK
Sbjct: 79  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 138

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE
Sbjct: 139 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 198

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
           QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL
Sbjct: 199 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 258

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 259 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 317

[Close]

BLAST of Cucsa.366410.1 vs. NCBI nr
Match: gi|700206934|gb|KGN62053.1| (hypothetical protein Csa_2G292830 [Cucumis sativus])

HSP 1 Score: 627.5 bits (1617), Expect = 1.2e-176
Identity = 298/299 (99.67%), Postives = 299/299 (100.00%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT
Sbjct: 42  YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 101

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK
Sbjct: 102 YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 161

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE
Sbjct: 162 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 221

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
           QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL
Sbjct: 222 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 281

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTKD 300
           NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTD+QGTCGIAMMASYPTKD
Sbjct: 282 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDRQGTCGIAMMASYPTKD 340

[Close]

BLAST of Cucsa.366410.1 vs. NCBI nr
Match: gi|659117224|ref|XP_008458487.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 568.9 bits (1465), Expect = 5.1e-159
Identity = 271/301 (90.03%), Postives = 284/301 (94.35%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           YQKWMDKYGRQYKSREEWE+RFTIYQANVQYIDNFNS+NHS+TLAENNF DLTNEEF AT
Sbjct: 46  YQKWMDKYGRQYKSREEWEQRFTIYQANVQYIDNFNSLNHSYTLAENNFTDLTNEEFMAT 105

Query: 61  YLGYKTVSIPDT--CFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGI 120
           YLGY+TVS+PDT   FRYGNMVNLPTNVDWR+EGAVTPIKNQGQCGSCWAFSAVAAVEGI
Sbjct: 106 YLGYETVSLPDTDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQGQCGSCWAFSAVAAVEGI 165

Query: 121 NKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESAC 180
           NKIKAGKL+SLSEQELVDCDVTSGNQGCNGGYMYKAFEFIK+TGLTTE+EYPY    SAC
Sbjct: 166 NKIKAGKLMSLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKKTGLTTELEYPYTATRSAC 225

Query: 181 NEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGN 240
           ++QKEKYQ VSISGYEKVPVNDEKSL+AAVA QPVSVAIDA GN+FQFYSGGIFSGNCG 
Sbjct: 226 DKQKEKYQSVSISGYEKVPVNDEKSLQAAVAKQPVSVAIDAGGNDFQFYSGGIFSGNCGK 285

Query: 241 QLNHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 300
           QLNHGVAIVGYGE SNQAYWLVKNSWGT WGESGYIRM RDSTDKQGTCGIAMMASYP K
Sbjct: 286 QLNHGVAIVGYGEDSNQAYWLVKNSWGTSWGESGYIRMMRDSTDKQGTCGIAMMASYPIK 345

[Close]

BLAST of Cucsa.366410.1 vs. NCBI nr
Match: gi|645245974|ref|XP_008229136.1| (PREDICTED: zingipain-2 [Prunus mume])

HSP 1 Score: 426.0 bits (1094), Expect = 5.4e-116
Identity = 197/298 (66.11%), Postives = 236/298 (79.19%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W+ KYGR YK+REE E RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T
Sbjct: 45  YERWLQKYGRIYKNREEAEYRFGVYKSNIEFVDFVNSQNQSYKLTDNKFADITNLEFTNT 104

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           ++G++T S P T F Y    +LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+
Sbjct: 105 FMGFQTRSHPKTKFSYDKDEDLPTAVDWRKNGAVTPIKNQGQCGSCWAFSAVAAVEGINQ 164

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK GKL+SLSEQELVDCDV +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E
Sbjct: 165 IKTGKLVSLSEQELVDCDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDE 224

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
              K   V+ISGYE +P N EKSL+AAVA+QPVSVA+DA    FQFYS GIF+G CG  L
Sbjct: 225 DSLKNHAVNISGYESIPANSEKSLQAAVAHQPVSVAVDAASYAFQFYSSGIFTGQCGKNL 284

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           NHGV  VGYGE S + YW+VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 285 NHGVTAVGYGEDSGKKYWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

[Close]

BLAST of Cucsa.366410.1 vs. NCBI nr
Match: gi|595908837|ref|XP_007214244.1| (hypothetical protein PRUPE_ppa023515mg [Prunus persica])

HSP 1 Score: 422.9 bits (1086), Expect = 4.6e-115
Identity = 196/298 (65.77%), Postives = 234/298 (78.52%), Query Frame = 1


Query: 1   YQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSHTLAENNFADLTNEEFKAT 60
           Y++W+ KYGR YK+REE   RF +Y++N++++D  NS N S+ L +N FAD+TN EF  T
Sbjct: 45  YERWLQKYGRIYKNREEAAYRFGVYKSNIEFVDFVNSQNLSYKLTDNKFADITNLEFTKT 104

Query: 61  YLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQCGSCWAFSAVAAVEGINK 120
           ++G++T S P T F Y     LPT VDWR+ GAVTPIKNQGQCGSCWAFSAVAAVEGIN+
Sbjct: 105 FMGFQTRSHPKTKFSYDKDEELPTAVDWRKHGAVTPIKNQGQCGSCWAFSAVAAVEGINQ 164

Query: 121 IKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTGLTTEIEYPYQGAESACNE 180
           IK GKL+SLSEQELVDCDV +GN+GCNGGYM KAF FIK  GL+TE +YPY+G++  C+E
Sbjct: 165 IKTGKLVSLSEQELVDCDVKTGNEGCNGGYMEKAFSFIKDNGLSTEKDYPYKGSDGICDE 224

Query: 181 QKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGNNFQFYSGGIFSGNCGNQL 240
              K   V+ISGYE +P N EKSL+AAVA+QPVSVA+DA G  FQFYS G F+G CG  L
Sbjct: 225 DSLKNSAVNISGYESIPANSEKSLQAAVAHQPVSVAVDAAGYAFQFYSSGTFTGQCGKNL 284

Query: 241 NHGVAIVGYGETSNQAYWLVKNSWGTDWGESGYIRMKRDSTDKQGTCGIAMMASYPTK 299
           NHGV  VGYGE S + YW+VKNSWG DWGESGYIRM RDS DK+GTCGIAM ASYP K
Sbjct: 285 NHGVTAVGYGEDSGKKYWIVKNSWGPDWGESGYIRMTRDSVDKKGTCGIAMQASYPVK 342

The following BLAST results are available for this feature:

BLAST of Cucsa.366410.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CEP1_ARATH	1.9e-92	55.34	KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana GN=CEP1 PE=1 SV=...	[more]
XCP1_ARATH	2.4e-92	55.26	Cysteine protease XCP1 OS=Arabidopsis thaliana GN=XCP1 PE=1 SV=1	[more]
CYSEP_VIGMU	3.2e-92	55.02	Vignain OS=Vigna mungo PE=1 SV=1	[more]
SAG12_ARATH	5.4e-92	55.19	Senescence-specific cysteine protease SAG12 OS=Arabidopsis thaliana GN=SAG12 PE=...	[more]
SAG39_ORYSI	2.1e-91	55.88	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica GN=OsI...	[more]

BLAST of Cucsa.366410.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJV6_CUCSA	8.5e-177	99.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G292830 PE=3 SV=1	[more]
M5X1M2_PRUPE	3.2e-115	65.77	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023515mg PE=3 SV=1	[more]
W9RAD3_9ROSA	8.1e-111	62.88	KDEL-tailed cysteine endopeptidase CEP2 OS=Morus notabilis GN=L484_001450 PE=3 S...	[more]
G7ZUL7_MEDTR	2.4e-110	63.21	Cysteine proteinase OS=Medicago truncatula GN=MTR_2g090890 PE=3 SV=1	[more]
A0A151TPE0_CAJCA	1.1e-107	63.12	Vignain OS=Cajanus cajan GN=KK1_022504 PE=3 SV=1	[more]

BLAST of Cucsa.366410.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G06260.1	1.0e-96	56.77	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	1.1e-93	55.34	Cysteine proteinases superfamily protein	[more]
AT4G35350.1	1.4e-93	55.26	xylem cysteine peptidase 1	[more]
AT5G45890.1	3.1e-93	55.19	senescence-associated gene 12	[more]
AT3G48340.1	4.4e-92	53.72	Cysteine proteinases superfamily protein	[more]

BLAST of Cucsa.366410.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449460678\|ref\|XP_004148072.1\|	1.2e-176	99.67	PREDICTED: ervatamin-B-like [Cucumis sativus]	[more]
gi\|700206934\|gb\|KGN62053.1\|	1.2e-176	99.67	hypothetical protein Csa_2G292830 [Cucumis sativus]	[more]
gi\|659117224\|ref\|XP_008458487.1\|	5.1e-159	90.03	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]
gi\|645245974\|ref\|XP_008229136.1\|	5.4e-116	66.11	PREDICTED: zingipain-2 [Prunus mume]	[more]
gi\|595908837\|ref\|XP_007214244.1\|	4.6e-115	65.77	hypothetical protein PRUPE_ppa023515mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000169	Pept_cys_AS
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A
IPR013201	Prot_inhib_I29
IPR025660	Pept_his_AS
IPR025661	Pept_asp_AS

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0008234	cysteine-type peptidase activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008234	cysteine-type peptidase activity
molecular_function	GO:0016491	oxidoreductase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.366410	Cucsa.366410	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.366410.1	Cucsa.366410.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.366410.1.CDS.2	Cucsa.366410.1.CDS.2	CDS
Cucsa.366410.1.CDS.1	Cucsa.366410.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 100..111 scor
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 242..252 score: 8.4E-11coord: 100..115 score: 8.4E-11coord: 257..263 score: 8.4
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 82..297 score: 3.5
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 82..297 score: 1.7E
IPR013128	Peptidase C1A	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 1..296 score: 5.9E
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 1..57 score: 1.1
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 1..57 score: 4.4
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 240..250 scor
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 257..276 scor
None	No IPR available	GENE3D	G3DSA:3.90.70.10		coord: 1..297 score: 6.6E
None	No IPR available	PANTHER	PTHR12411:SF382	CYSTEINE PROTEINASES SUPERFAMILY PROTEIN	coord: 1..296 score: 5.9E
None	No IPR available	unknown	SSF54001	Cysteine proteinases	coord: 1..297 score: 1.12E