Cucsa.321700 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.321700 vs. Swiss-Prot
Match: PP420_ARATH (Pentatricopeptide repeat-containing protein At5g46580, chloroplastic OS=Arabidopsis thaliana GN=At5g46580 PE=2 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 2.5e-283
Identity = 463/664 (69.73%), Postives = 566/664 (85.24%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           P ++  + +  K PSLSEQLK LS TTL     ++T++LSKPKS WVNPT+PKRSVLSLQ
Sbjct: 50  PKTLEEEPITTKTPSLSEQLKPLSATTLR---QEQTQILSKPKSVWVNPTRPKRSVLSLQ 109

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           RQKRS+YSYNP+++DL++FA KLN+   ++ + F++ L+EIPHPP ++NALL+LNSLR W
Sbjct: 110 RQKRSAYSYNPQIKDLRAFALKLNSSIFTEKSEFLSLLDEIPHPPNRDNALLVLNSLREW 169

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
           QKTH FFNW+KS++LFPMETIFYNV MKSLR+GRQFQLIE++A EM+  G+ELDNITYST
Sbjct: 170 QKTHTFFNWVKSKSLFPMETIFYNVTMKSLRFGRQFQLIEEMALEMVKDGVELDNITYST 229

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAK+C+ ++KA+EWFERMYKTGLMPDEVTYSAILDVY+  GKVEEVLSLYER  A+G
Sbjct: 230 IITCAKRCNLYNKAIEWFERMYKTGLMPDEVTYSAILDVYSKSGKVEEVLSLYERAVATG 289

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           W PD   FSVLGKMFGEAGDYDGI YVLQEMKS++++PN+VVYNTLL+AMG+AGKPG AR
Sbjct: 290 WKPDAIAFSVLGKMFGEAGDYDGIRYVLQEMKSMDVKPNVVVYNTLLEAMGRAGKPGLAR 349

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLF+EM+E+G+TPNEKTLTALVKIYGKARWARDAL LWE M++  WPMDFILYNTLLNMC
Sbjct: 350 SLFNEMLEAGLTPNEKTLTALVKIYGKARWARDALQLWEEMKAKKWPMDFILYNTLLNMC 409

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           AD+GLEEEAE LF +MK+S   RPD++SYTAMLNIYGSGG  +++MELFEEML+ GV++N
Sbjct: 410 ADIGLEEEAERLFNDMKESVQCRPDNFSYTAMLNIYGSGGKAEKAMELFEEMLKAGVQVN 469

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VM CTCL+QCLGK+ RIDD+V VF++S+++G+KPDDRLCGCLLSV++LC +SED  KV  
Sbjct: 470 VMGCTCLVQCLGKAKRIDDVVYVFDLSIKRGVKPDDRLCGCLLSVMALCESSEDAEKVMA 529

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CL++AN KLV+F+NL+      +E VK EF+ ++  T  EARRPFCNCLIDICR  N  E
Sbjct: 530 CLERANKKLVTFVNLIVDEKTEYETVKEEFKLVINATQVEARRPFCNCLIDICRGNNRHE 589

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLG+L+GLYPGLHNKT  EW LDVRSLSVGAA+TALEEWM TL+ I++R+E LP
Sbjct: 590 RAHELLYLGTLFGLYPGLHNKTIKEWSLDVRSLSVGAAETALEEWMRTLANIIKRQEELP 649

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           EL  AQTG GTHRFSQGLANSFA H+ +L+APF+ + DR G FVAT+EDLV+W+ S+ P 
Sbjct: 650 ELFLAQTGTGTHRFSQGLANSFALHLQQLSAPFR-QSDRPGIFVATKEDLVSWLESKFPP 709

Query: 661 VAAT 665
           +  +
Sbjct: 710 LVTS 709

BLAST of Cucsa.321700 vs. Swiss-Prot
Match: PP314_ARATH (Pentatricopeptide repeat-containing protein At4g16390, chloroplastic OS=Arabidopsis thaliana GN=P67 PE=1 SV=3)

HSP 1 Score: 442.2 bits (1136), Expect = 1.0e-122
Identity = 236/615 (38.37%), Postives = 365/615 (59.35%), Query Frame = 1

Query: 46  WVNPTKPKRSVLSLQRQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPP 105
           WVNP  P+ S L   R+K    SY+ +   L   A  L+AC   ++A     +       
Sbjct: 89  WVNPKSPRASQL---RRK----SYDSRYSSLIKLAESLDAC-KPNEADVCDVITGFGGKL 148

Query: 106 TKENALLILNSLRPWQKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANE 165
            +++A++ LN++   +   L  N +        E I YNV MK  R  +  +  E L +E
Sbjct: 149 FEQDAVVTLNNMTNPETAPLVLNNLLETMKPSREVILYNVTMKVFRKSKDLEKSEKLFDE 208

Query: 166 MISAGIELDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGK 225
           M+  GI+ DN T++TII+CA++     +A+EWFE+M   G  PD VT +A++D Y   G 
Sbjct: 209 MLERGIKPDNATFTTIISCARQNGVPKRAVEWFEKMSSFGCEPDNVTMAAMIDAYGRAGN 268

Query: 226 VEEVLSLYERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNT 285
           V+  LSLY+R R   W  D  TFS L +++G +G+YDG + + +EMK++ ++PNLV+YN 
Sbjct: 269 VDMALSLYDRARTEKWRIDAVTFSTLIRIYGVSGNYDGCLNIYEEMKALGVKPNLVIYNR 328

Query: 286 LLDAMGKAGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNG 345
           L+D+MG+A +P  A+ ++ +++ +G TPN  T  ALV+ YG+AR+  DAL ++  M+  G
Sbjct: 329 LIDSMGRAKRPWQAKIIYKDLITNGFTPNWSTYAALVRAYGRARYGDDALAIYREMKEKG 388

Query: 346 WPMDFILYNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRS 405
             +  ILYNTLL+MCAD    +EA  +F++MK  +   PDSW++++++ +Y   G V  +
Sbjct: 389 LSLTVILYNTLLSMCADNRYVDEAFEIFQDMKNCETCDPDSWTFSSLITVYACSGRVSEA 448

Query: 406 MELFEEMLELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSV 465
                +M E G E  +   T +IQC GK+ ++DD+VR F+  ++ GI PDDR CGCLL+V
Sbjct: 449 EAALLQMREAGFEPTLFVLTSVIQCYGKAKQVDDVVRTFDQVLELGITPDDRFCGCLLNV 508

Query: 466 LSLCYNSEDINKVFTCLQQANPKLVSFINLL-QQNDITFEVVKNEFRNILGETAPEARRP 525
           ++    SE+I K+  C+++A PKL   + +L ++ +    V K E   ++     + ++ 
Sbjct: 509 MTQT-PSEEIGKLIGCVEKAKPKLGQVVKMLVEEQNCEEGVFKKEASELIDSIGSDVKKA 568

Query: 526 FCNCLIDICRNQNLRERAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEE 585
           + NCLID+C N N  ERA E+L LG  Y +Y GL +K+ T+W L ++SLS+GAA TAL  
Sbjct: 569 YLNCLIDLCVNLNKLERACEILQLGLEYDIYTGLQSKSATQWSLHLKSLSLGAALTALHV 628

Query: 586 WMITLSK-IVQREEALPELLSAQTGAGTHRFS-QGLANSFASHVDKLAAPFQLREDRAGW 645
           WM  LS+  ++  E  P LL   TG G H++S +GLA  F SH+ +L APF    D+ GW
Sbjct: 629 WMNDLSEAALESGEEFPPLLGINTGHGKHKYSDKGLAAVFESHLKELNAPFHEAPDKVGW 688

Query: 646 FVATREDLVTWVHSR 658
           F+ T      W+ SR
Sbjct: 689 FLTTSVAAKAWLESR 694

BLAST of Cucsa.321700 vs. Swiss-Prot
Match: PPR49_ARATH (Pentatricopeptide repeat-containing protein At1g18900 OS=Arabidopsis thaliana GN=At1g18900 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.3e-41
Identity = 116/492 (23.58%), Postives = 229/492 (46.54%), Query Frame = 1

Query: 170 GIELDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEV 229
           G + D  TY+T++    +  +F    +  + M + G  P+ VTY+ ++  Y     + E 
Sbjct: 359 GFKHDGHTYTTMVGNLGRAKQFGAINKLLDEMVRDGCQPNTVTYNRLIHSYGRANYLNEA 418

Query: 230 LSLYERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDA 289
           ++++ + + +G  PD  T+  L  +  +AG  D  M + Q M++  + P+   Y+ +++ 
Sbjct: 419 MNVFNQMQEAGCKPDRVTYCTLIDIHAKAGFLDIAMDMYQRMQAGGLSPDTFTYSVIINC 478

Query: 290 MGKAGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMD 349
           +GKAG    A  LF EMV+ G TPN  T   ++ ++ KAR  ++AL L+  M++ G+  D
Sbjct: 479 LGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMMDLHAKARNYQNALKLYRDMQNAGFEPD 538

Query: 350 FILYNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELF 409
            + Y+ ++ +    G  EEAE +F EM++ K+  PD   Y  +++++G  GNV+++ + +
Sbjct: 539 KVTYSIVMEVLGHCGYLEEAEAVFTEMQQ-KNWIPDEPVYGLLVDLWGKAGNVEKAWQWY 598

Query: 410 EEMLELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLC 469
           + ML  G+  NV  C  L+    +  +I +   +    +  G++P  +    LLS  +  
Sbjct: 599 QAMLHAGLRPNVPTCNSLLSTFLRVNKIAEAYELLQNMLALGLRPSLQTYTLLLSCCTDG 658

Query: 470 YNSEDINKVFTCLQQANPKLVSFINLLQQNDITFEVVK---NEFRNILGETAPEARRPFC 529
            +  D+      +         F+  +       E V+   N F +++     E++R   
Sbjct: 659 RSKLDMGFCGQLMASTGHPAHMFLLKMPAAGPDGENVRNHANNFLDLMHSEDRESKRGLV 718

Query: 530 NCLIDICRNQNLRERAHELLYLGSLYGLYP-GLHNKTETEWCLDVRSLSVGAAQTALEEW 589
           + ++D       +E A  +  + +   ++P  L  K+ + W +++  +S G A TAL   
Sbjct: 719 DAVVDFLHKSGQKEEAGSVWEVAAQKNVFPDALREKSCSYWLINLHVMSEGTAVTALSRT 778

Query: 590 MITLSKIVQREEALPELLSAQTGAGTHRFSQG---LANSFASHVDKLAAPFQLREDRAGW 649
           +    K +      P  +   TG G      G   +  +    ++   +PF      +G 
Sbjct: 779 LAWFRKQMLASGTCPSRIDIVTGWGRRSRVTGTSMVRQAVEELLNIFGSPFFTESGNSGC 838

Query: 650 FVATREDLVTWV 655
           FV + E L  W+
Sbjct: 839 FVGSGEPLNRWL 849

HSP 2 Score: 163.3 bits (412), Expect = 9.1e-39
Identity = 125/546 (22.89%), Postives = 236/546 (43.22%), Query Frame = 1

Query: 113 ILNSLRPWQKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIE 172
           +L  +  +     FF W+K Q  F  +   Y   + +L   +QF  I  L +EM+  G +
Sbjct: 337 VLKQMNDYGNALGFFYWLKRQPGFKHDGHTYTTMVGNLGRAKQFGAINKLLDEMVRDGCQ 396

Query: 173 LDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSL 232
            + +TY+ +I    + +  ++AM  F +M + G  PD VTY  ++D++A  G ++  + +
Sbjct: 397 PNTVTYNRLIHSYGRANYLNEAMNVFNQMQEAGCKPDRVTYCTLIDIHAKAGFLDIAMDM 456

Query: 233 YERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGK 292
           Y+R +A G +PD +T+SV+    G+AG       +  EM      PNLV YN ++D   K
Sbjct: 457 YQRMQAGGLSPDTFTYSVIINCLGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMMDLHAK 516

Query: 293 AGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFIL 352
           A     A  L+ +M  +G  P++ T + ++++ G   +  +A  ++  M+   W  D  +
Sbjct: 517 ARNYQNALKLYRDMQNAGFEPDKVTYSIVMEVLGHCGYLEEAEAVFTEMQQKNWIPDEPV 576

Query: 353 YNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEM 412
           Y  L+++    G  E+A   ++ M  +   RP+  +  ++L+ +     +  + EL + M
Sbjct: 577 YGLLVDLWGKAGNVEKAWQWYQAMLHA-GLRPNVPTCNSLLSTFLRVNKIAEAYELLQNM 636

Query: 413 LELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNS 472
           L LG+  ++   T L+ C    GR                K D   CG L++        
Sbjct: 637 LALGLRPSLQTYTLLLSCC-TDGR---------------SKLDMGFCGQLMA-----STG 696

Query: 473 EDINKVFTCLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDI 532
              +     +  A P   +  N             N F +++     E++R   + ++D 
Sbjct: 697 HPAHMFLLKMPAAGPDGENVRN-----------HANNFLDLMHSEDRESKRGLVDAVVDF 756

Query: 533 CRNQNLRERAHELLYLGSLYGLYP-GLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSK 592
                 +E A  +  + +   ++P  L  K+ + W +++  +S G A TAL   +    K
Sbjct: 757 LHKSGQKEEAGSVWEVAAQKNVFPDALREKSCSYWLINLHVMSEGTAVTALSRTLAWFRK 816

Query: 593 IVQREEALPELLSAQTGAGTHRFSQG---LANSFASHVDKLAAPFQLREDRAGWFVATRE 652
            +      P  +   TG G      G   +  +    ++   +PF      +G FV + E
Sbjct: 817 QMLASGTCPSRIDIVTGWGRRSRVTGTSMVRQAVEELLNIFGSPFFTESGNSGCFVGSGE 849

Query: 653 DLVTWV 655
            L  W+
Sbjct: 877 PLNRWL 849

BLAST of Cucsa.321700 vs. Swiss-Prot
Match: PP123_ARATH (Pentatricopeptide repeat-containing protein At1g74750 OS=Arabidopsis thaliana GN=At1g74750 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 6.3e-40
Identity = 116/492 (23.58%), Postives = 225/492 (45.73%), Query Frame = 1

Query: 170 GIELDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEV 229
           G + D  TY+T++    +  +F +  +  + M + G  P+ VTY+ ++  Y     ++E 
Sbjct: 354 GFKHDGHTYTTMVGNLGRAKQFGEINKLLDEMVRDGCKPNTVTYNRLIHSYGRANYLKEA 413

Query: 230 LSLYERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDA 289
           ++++ + + +G  PD  T+  L  +  +AG  D  M + Q M+   + P+   Y+ +++ 
Sbjct: 414 MNVFNQMQEAGCEPDRVTYCTLIDIHAKAGFLDIAMDMYQRMQEAGLSPDTFTYSVIINC 473

Query: 290 MGKAGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMD 349
           +GKAG    A  LF EMV  G TPN  T   ++ ++ KAR    AL L+  M++ G+  D
Sbjct: 474 LGKAGHLPAAHRLFCEMVGQGCTPNLVTFNIMIALHAKARNYETALKLYRDMQNAGFQPD 533

Query: 350 FILYNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELF 409
            + Y+ ++ +    G  EEAE +F EM++ K+  PD   Y  +++++G  GNV ++ + +
Sbjct: 534 KVTYSIVMEVLGHCGFLEEAEGVFAEMQR-KNWVPDEPVYGLLVDLWGKAGNVDKAWQWY 593

Query: 410 EEMLELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLC 469
           + ML+ G+  NV  C  L+    +  R+ +   +    +  G+ P  +    LLS  +  
Sbjct: 594 QAMLQAGLRPNVPTCNSLLSTFLRVHRMSEAYNLLQSMLALGLHPSLQTYTLLLSCCTDA 653

Query: 470 YNSEDINKVFTCLQQANPKLVSFINLLQQNDITFEVVKNEFRNILG---ETAPEARRPFC 529
            ++ D+      +  +      F+  +       + V++   N L        E++R   
Sbjct: 654 RSNFDMGFCGQLMAVSGHPAHMFLLKMPPAGPDGQKVRDHVSNFLDFMHSEDRESKRGLM 713

Query: 530 NCLIDICRNQNLRERAHELLYLGSLYGLYP-GLHNKTETEWCLDVRSLSVGAAQTALEEW 589
           + ++D      L+E A  +  + +   +YP  L  K+ + W +++  +S G A  AL   
Sbjct: 714 DAVVDFLHKSGLKEEAGSVWEVAAGKNVYPDALREKSYSYWLINLHVMSEGTAVIALSRT 773

Query: 590 MITLSKIVQREEALPELLSAQTGAGTHRFSQG---LANSFASHVDKLAAPFQLREDRAGW 649
           +    K +      P  +   TG G      G   +  +    ++    PF      +G 
Sbjct: 774 LAWFRKQMLVSGDCPSRIDIVTGWGRRSRVTGTSMVRQAVEELLNIFNFPFFTENGNSGC 833

Query: 650 FVATREDLVTWV 655
           FV + E L  W+
Sbjct: 834 FVGSGEPLKNWL 844

BLAST of Cucsa.321700 vs. Swiss-Prot
Match: PP124_ARATH (Pentatricopeptide repeat-containing protein At1g74850, chloroplastic OS=Arabidopsis thaliana GN=PTAC2 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 2.0e-38
Identity = 134/586 (22.87%), Postives = 245/586 (41.81%), Query Frame = 1

Query: 120 WQKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYS 179
           W+     F  ++ + + P + + YN  + +          E +   M   GI  D  TYS
Sbjct: 228 WEGLLGLFAEMRHEGIQP-DIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYS 287

Query: 180 TIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRAS 239
            ++    K  R +K  +    M   G +PD  +Y+ +L+ YA  G ++E + ++ + +A+
Sbjct: 288 HLVETFGKLRRLEKVCDLLGEMASGGSLPDITSYNVLLEAYAKSGSIKEAMGVFHQMQAA 347

Query: 240 GWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFA 299
           G TP+  T+SVL  +FG++G YD +  +  EMKS    P+   YN L++  G+ G     
Sbjct: 348 GCTPNANTYSVLLNLFGQSGRYDDVRQLFLEMKSSNTDPDAATYNILIEVFGEGGYFKEV 407

Query: 300 RSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNM 359
            +LF +MVE  I P+ +T   ++   GK     DA  + + M +N        Y  ++  
Sbjct: 408 VTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTANDIVPSSKAYTGVIEA 467

Query: 360 CADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEI 419
                L EEA   F  M +   S P   ++ ++L  +  GG VK S  +   +++ G+  
Sbjct: 468 FGQAALYEEALVAFNTMHE-VGSNPSIETFHSLLYSFARGGLVKESEAILSRLVDSGIPR 527

Query: 420 NVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVF 479
           N       I+   + G+ ++ V+ +    +    PD+R    +LSV S     ++  + F
Sbjct: 528 NRDTFNAQIEAYKQGGKFEEAVKTYVDMEKSRCDPDERTLEAVLSVYSFARLVDECREQF 587

Query: 480 TCLQQAN--PKLVSFINLL----------QQNDITFEVVKNEFRNI-------------- 539
             ++ ++  P ++ +  +L            N++  E++ N   NI              
Sbjct: 588 EEMKASDILPSIMCYCMMLAVYGKTERWDDVNELLEEMLSNRVSNIHQVIGQMIKGDYDD 647

Query: 540 -------------LGETAPEARRPFCNCLIDICRNQNLRERAHELLYLGSLYGLYPGLHN 599
                        L          F N L+D       +ERA  +L   +  GL+P L  
Sbjct: 648 DSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGLFPELFR 707

Query: 600 KTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPELLSAQTGAGTHRFSQGLAN 659
           K +  W +DV  +S G   TAL  W+  ++ ++ + + LP+L    +  G    S     
Sbjct: 708 KNKLVWSVDVHRMSEGGMYTALSVWLNDINDMLLKGD-LPQLAVVVSVRGQLEKSSAARE 767

Query: 660 S------FASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 661
           S      F+   D +++ F       G  +  R  L   + ++ P+
Sbjct: 768 SPIAKAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLKQLLSTKEPT 810

HSP 2 Score: 128.6 bits (322), Expect = 2.5e-28
Identity = 81/279 (29.03%), Postives = 139/279 (49.82%), Query Frame = 1

Query: 178 YSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGR 237
           Y+ +I+   +    DK +E F+ M   G+     +Y+A+++ Y   G+ E  L L +R +
Sbjct: 144 YTIMISLLGREGLLDKCLEVFDEMPSQGVSRSVFSYTALINAYGRNGRYETSLELLDRMK 203

Query: 238 ASGWTPDPYTFSVLGKMFGEAG-DYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKP 297
               +P   T++ +       G D++G++ +  EM+   +QP++V YNTLL A    G  
Sbjct: 204 NEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEGIQPDIVTYNTLLSACAIRGLG 263

Query: 298 GFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTL 357
             A  +F  M + GI P+  T + LV+ +GK R      DL   M S G   D   YN L
Sbjct: 264 DEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLRRLEKVCDLLGEMASGGSLPDITSYNVL 323

Query: 358 LNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELG 417
           L   A  G  +EA  +F +M ++    P++ +Y+ +LN++G  G      +LF EM    
Sbjct: 324 LEAYAKSGSIKEAMGVFHQM-QAAGCTPNANTYSVLLNLFGQSGRYDDVRQLFLEMKSSN 383

Query: 418 VEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPD 456
            + +      LI+  G+ G   ++V +F+  V++ I+PD
Sbjct: 384 TDPDAATYNILIEVFGEGGYFKEVVTLFHDMVEENIEPD 421

HSP 3 Score: 55.1 bits (131), Expect = 3.5e-06
Identity = 42/206 (20.39%), Postives = 94/206 (45.63%), Query Frame = 1

Query: 75  DLKSFAHKLNAC-------DSSDDASFIAALEEIPHPPTKENALLILNSLRPWQKTHLFF 134
           D++++   + AC       D+     ++ A + +P        +        +++  + F
Sbjct: 421 DMETYEGIIFACGKGGLHEDARKILQYMTANDIVPSSKAYTGVIEAFGQAALYEEALVAF 480

Query: 135 NWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTIITCAKK 194
           N +      P    F+++ + S   G   +  E + + ++ +GI  +  T++  I   K+
Sbjct: 481 NTMHEVGSNPSIETFHSL-LYSFARGGLVKESEAILSRLVDSGIPRNRDTFNAQIEAYKQ 540

Query: 195 CSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWTPDPYT 254
             +F++A++ +  M K+   PDE T  A+L VY+    V+E    +E  +AS   P    
Sbjct: 541 GGKFEEAVKTYVDMEKSRCDPDERTLEAVLSVYSFARLVDECREQFEEMKASDILPSIMC 600

Query: 255 FSVLGKMFGEAGDYDGIMYVLQEMKS 274
           + ++  ++G+   +D +  +L+EM S
Sbjct: 601 YCMMLAVYGKTERWDDVNELLEEMLS 625

BLAST of Cucsa.321700 vs. TrEMBL
Match: A0A0A0L6K8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G011820 PE=4 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 665/665 (100.00%), Postives = 665/665 (100.00%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ
Sbjct: 46  PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 105

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW
Sbjct: 106 RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 165

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
           QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST
Sbjct: 166 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 225

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG
Sbjct: 226 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 285

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR
Sbjct: 286 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 345

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC
Sbjct: 346 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 405

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN
Sbjct: 406 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 465

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT
Sbjct: 466 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 525

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE
Sbjct: 526 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 585

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP
Sbjct: 586 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 645

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS
Sbjct: 646 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 705

Query: 661 VAATA 666
           VAATA
Sbjct: 706 VAATA 710

BLAST of Cucsa.321700 vs. TrEMBL
Match: F6HTA5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_02s0012g01090 PE=4 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 5.5e-309
Identity = 516/663 (77.83%), Postives = 590/663 (88.99%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           P+S  S+  +++NPSLSEQLK LS T L+   + +T L+SKPKSTW+NPTKPK SVLSLQ
Sbjct: 60  PTSSDSEQTNHQNPSLSEQLKPLSKTILTRDHSGQTHLVSKPKSTWINPTKPKPSVLSLQ 119

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           R KR +YSYNP++RDLK FA K+N  +SSD++ F+A LE+IPHPPT++NALL+LNSL+PW
Sbjct: 120 RHKRHNYSYNPQIRDLKLFAKKINESESSDESEFLAVLEQIPHPPTRDNALLLLNSLKPW 179

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
            KT+LFFNWIK+QNLFPMETIFYNV MKSLR+GRQFQLIE+LANEMIS G+ELDNITYST
Sbjct: 180 PKTYLFFNWIKTQNLFPMETIFYNVTMKSLRFGRQFQLIEELANEMISTGVELDNITYST 239

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAK+C+ FDKA++WFERMYKTGLMPDEVTYSAILDVYA LGKVEEVLSLYERGRASG
Sbjct: 240 IITCAKRCNLFDKAVKWFERMYKTGLMPDEVTYSAILDVYAKLGKVEEVLSLYERGRASG 299

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           W PDP  F+VLGKMFGEAGDYDGI YVLQEMKS+ +QPNLVVYNTLL+AMGKAGKPG AR
Sbjct: 300 WKPDPIAFAVLGKMFGEAGDYDGIRYVLQEMKSLGVQPNLVVYNTLLEAMGKAGKPGLAR 359

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLF+EMV SG+ P+ KTLTALVKIYGKARWARDAL+LWERMRSNGWPMDFILYNTLL+MC
Sbjct: 360 SLFEEMVGSGVIPDAKTLTALVKIYGKARWARDALELWERMRSNGWPMDFILYNTLLSMC 419

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           ADLGLEEEAE LFE+MKKS+H RPDSWSYTAMLNIYGSGGNV R+M+LF+EM ELGV+IN
Sbjct: 420 ADLGLEEEAEKLFEDMKKSEHCRPDSWSYTAMLNIYGSGGNVDRAMQLFDEMSELGVQIN 479

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VM CTCL QCLG++ RIDDLV+VF VS+++G+KPDDRLCGCLLSV+S C  +ED NKV  
Sbjct: 480 VMGCTCLSQCLGRARRIDDLVKVFEVSLERGVKPDDRLCGCLLSVVSFCEGAEDANKVLA 539

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CLQQANPKLV+F+NLL++  I+FE +K EFR IL +TA EARRPFCNCLIDICRN++L E
Sbjct: 540 CLQQANPKLVAFVNLLEEK-ISFEALKEEFRGILTDTAVEARRPFCNCLIDICRNRSLHE 599

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLG+LYGLYPGLHN+T  EWCLDVRSLSVGAA TALEEWM TLSKIVQREEALP
Sbjct: 600 RAHELLYLGTLYGLYPGLHNRTADEWCLDVRSLSVGAAHTALEEWMGTLSKIVQREEALP 659

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           E  SA TG GTH+FSQGLA++FASHV KLAAPF   E++AG FVATREDLV+WV SR+ S
Sbjct: 660 EAFSANTGTGTHKFSQGLASAFASHVKKLAAPFTQSEEKAGCFVATREDLVSWVQSRILS 719

Query: 661 VAA 664
            AA
Sbjct: 720 PAA 721

BLAST of Cucsa.321700 vs. TrEMBL
Match: M5WQE9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002049mg PE=4 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 6.7e-307
Identity = 514/658 (78.12%), Postives = 585/658 (88.91%), Query Frame = 1

Query: 10  DNKNPSLS--EQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQRQKRSSY 69
           + KNPSLS  EQL+ L++TTLSN P D+++LLSKPKS WVNP KPKRSVLSLQRQKRS Y
Sbjct: 68  NKKNPSLSLSEQLQPLTSTTLSNPPKDQSQLLSKPKSIWVNPAKPKRSVLSLQRQKRSLY 127

Query: 70  SYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPWQKTHLFF 129
           SYNP++RDL+ FAHKLN CD+S +A F+AALEEIPHPPT+ENALLILNSL+PWQKTH+FF
Sbjct: 128 SYNPQVRDLRQFAHKLNDCDASQNA-FLAALEEIPHPPTRENALLILNSLKPWQKTHMFF 187

Query: 130 NWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTIITCAKK 189
           NW+K+QN FPM+TIFYNV MKSLR+GRQFQLIE+LA EM+S  IELDNITYSTIITCAK+
Sbjct: 188 NWVKAQNSFPMDTIFYNVTMKSLRFGRQFQLIEELAEEMVSNEIELDNITYSTIITCAKR 247

Query: 190 CSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWTPDPYT 249
              FDKA+EWFERMYKTGLMPDEVTYSAILDVYA LGKVEEVLSLYERGRASGW PDP  
Sbjct: 248 SKLFDKAVEWFERMYKTGLMPDEVTYSAILDVYAKLGKVEEVLSLYERGRASGWKPDPIA 307

Query: 250 FSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFARSLFDEMV 309
           FSVLGKMFGEAGDYDGI YVLQEM ++ +QPNLVVYNTLL+AMGKAGKPG ARSLF+EMV
Sbjct: 308 FSVLGKMFGEAGDYDGIRYVLQEMAALGVQPNLVVYNTLLEAMGKAGKPGLARSLFEEMV 367

Query: 310 ESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMCADLGLEE 369
            SG+ PNEKTLTALVKIYGKARWARDAL+LWERMRSN WPMDFILYNTLLNMCADLGLEE
Sbjct: 368 GSGLKPNEKTLTALVKIYGKARWARDALELWERMRSNEWPMDFILYNTLLNMCADLGLEE 427

Query: 370 EAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEINVMCCTCL 429
           EA+ LFE+MK+S+H RPDSWSYTAMLNI+GSGGNV  +M LFEEM ELG+E+NVM CTCL
Sbjct: 428 EAKKLFEDMKQSEHCRPDSWSYTAMLNIFGSGGNVDGAMGLFEEMSELGIELNVMGCTCL 487

Query: 430 IQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFTCLQQANP 489
           IQCLGK+ R  D+VRVF V+V++G+KPDDRLCGCLLSV+SLC  +ED +KV +CLQQANP
Sbjct: 488 IQCLGKARRFSDMVRVFGVAVERGVKPDDRLCGCLLSVVSLCEKTEDEDKVLSCLQQANP 547

Query: 490 KLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRERAHELLY 549
           KLV+ + +LQ   + FE +K+EFR+++  T+ E+RRPFCNCLIDICRN+N  ERAHELLY
Sbjct: 548 KLVTLVKVLQDKKLGFETIKDEFRDVISGTSVESRRPFCNCLIDICRNKNNHERAHELLY 607

Query: 550 LGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPELLSAQT 609
           LG+LYGLYPGLHNKT  EWCLDVRSLS+GAA TALEEWM TL KIVQREEALPEL SAQT
Sbjct: 608 LGTLYGLYPGLHNKTSREWCLDVRSLSIGAAHTALEEWMGTLYKIVQREEALPELFSAQT 667

Query: 610 GAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPSVAATA 666
           G GTH+FSQGLA+SFASHV+KLAAPF+  E++AG FVATREDLV+WV S+ PS A TA
Sbjct: 668 GTGTHKFSQGLAHSFASHVEKLAAPFRKSEEKAGRFVATREDLVSWVQSQAPSTAITA 724

BLAST of Cucsa.321700 vs. TrEMBL
Match: A0A067DT79_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g005178mg PE=4 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 7.1e-301
Identity = 508/659 (77.09%), Postives = 577/659 (87.56%), Query Frame = 1

Query: 3   SVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQRQ 62
           +  S + + K  SLSEQLK LS+TTLS   ND T LLSKPKSTWVNPTKP+RSVLSLQRQ
Sbjct: 51  AAESPNPETKTLSLSEQLKPLSSTTLSPTKNDRTPLLSKPKSTWVNPTKPRRSVLSLQRQ 110

Query: 63  KRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPWQK 122
           KRS+YSYNP++RDLK FA KLN CD++++A F+ A+ EIPH PT+ENALLILNSL+ WQK
Sbjct: 111 KRSTYSYNPRVRDLKLFARKLNDCDNTEEA-FLRAITEIPHQPTRENALLILNSLKFWQK 170

Query: 123 THLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTII 182
           ++ FFNWIKSQNLFPMETIFYNV MKSLR+GRQFQLIE LANEM+S  IELDNITYSTII
Sbjct: 171 SYFFFNWIKSQNLFPMETIFYNVTMKSLRFGRQFQLIEQLANEMVSNEIELDNITYSTII 230

Query: 183 TCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWT 242
           TCAK+C+ FD+A+EWFERMYKTGLMPDEVTYSAILDVYA  GKVEEVLSLYERG ASGW 
Sbjct: 231 TCAKRCNLFDEAIEWFERMYKTGLMPDEVTYSAILDVYAKSGKVEEVLSLYERGVASGWK 290

Query: 243 PDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFARSL 302
           PDP  FSVLGKMFGE+GDYDGI YVLQEMKS+ +QPNLVVYNTLL+AMGKAGKPG ARSL
Sbjct: 291 PDPIAFSVLGKMFGESGDYDGIRYVLQEMKSLGVQPNLVVYNTLLEAMGKAGKPGLARSL 350

Query: 303 FDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMCAD 362
           FDEMVESG+TP+EKTLTAL+KIYGKARWA+DAL+LWERMR N WPMDFILYNTLLNMCAD
Sbjct: 351 FDEMVESGLTPDEKTLTALIKIYGKARWAKDALELWERMRENKWPMDFILYNTLLNMCAD 410

Query: 363 LGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEINVM 422
           +GL EEAE LFE+MK S + +PD++SYTAMLNIYGSGGNV  ++ELFEEM ELGV INVM
Sbjct: 411 IGLVEEAERLFEDMKLSDYCKPDNYSYTAMLNIYGSGGNVDNAIELFEEMSELGVAINVM 470

Query: 423 CCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFTCL 482
            CTCLIQCLGK+ RIDDLVRVF VS+ +G+KPDDRLCGCLLSV+SLC  SED+ KV TCL
Sbjct: 471 GCTCLIQCLGKARRIDDLVRVFGVSIDRGVKPDDRLCGCLLSVVSLCETSEDVGKVITCL 530

Query: 483 QQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRERA 542
           QQANPKLV+F+NL++ N   FE +K EFRN++ +T  +ARRPFCNCLIDICRN+NL ERA
Sbjct: 531 QQANPKLVAFLNLIEDNSTGFENIKEEFRNVIKDTEVDARRPFCNCLIDICRNRNLNERA 590

Query: 543 HELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPEL 602
           HELLYLG+LYGLYPGLHNKT  EW LDVRSLSVGAAQTALEEWM TL+KIV REE LP+L
Sbjct: 591 HELLYLGTLYGLYPGLHNKTLDEWSLDVRSLSVGAAQTALEEWMWTLAKIVLREEVLPQL 650

Query: 603 LSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPSV 662
             A+TG GTH+FSQGLA +FASHV+KLAAPF+  E +AG FVATREDLV+WV +R  S+
Sbjct: 651 FLAETGTGTHKFSQGLATAFASHVNKLAAPFRQSEGKAGCFVATREDLVSWVQARPSSI 708

BLAST of Cucsa.321700 vs. TrEMBL
Match: A0A067KLE7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10617 PE=4 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 4.3e-298
Identity = 497/658 (75.53%), Postives = 575/658 (87.39%), Query Frame = 1

Query: 3   SVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQRQ 62
           +V   +   KNPSLS+QLK LS TTLS   +++T+LLSKPKSTWVNPT+PKRSVLSLQRQ
Sbjct: 56  NVKESANPKKNPSLSDQLKPLSATTLSTVKSNQTQLLSKPKSTWVNPTRPKRSVLSLQRQ 115

Query: 63  KRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPWQK 122
           KRS YS NP++++L+ FA KLN CDSS+ A F++ LE+IP+PPT+ENALLILNSL+PWQK
Sbjct: 116 KRSPYSLNPEVKELRLFAQKLNECDSSESA-FVSLLEQIPYPPTRENALLILNSLKPWQK 175

Query: 123 THLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTII 182
            +LFFNWIK+QNLFPMETIFYNV MKSLR+GRQF+LIE+LA EM+S  IELDNITYSTII
Sbjct: 176 AYLFFNWIKTQNLFPMETIFYNVIMKSLRFGRQFELIENLAYEMVSNKIELDNITYSTII 235

Query: 183 TCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWT 242
           TCAK+C+ FDKA+EWFERMYKTGLMPDEVTYSA LDVYA LGKVEEVLSLYERG ASGW 
Sbjct: 236 TCAKRCNLFDKALEWFERMYKTGLMPDEVTYSATLDVYAKLGKVEEVLSLYERGVASGWK 295

Query: 243 PDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFARSL 302
           PDP TFSVL +MFGEAGDYDGI YVLQEM+S+ +QPN+VVYNTLL+A+GKAGKPG ARSL
Sbjct: 296 PDPVTFSVLARMFGEAGDYDGIRYVLQEMESLGVQPNVVVYNTLLEALGKAGKPGLARSL 355

Query: 303 FDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMCAD 362
           F+EMV+SG+TPNEKT+TA+ KIYGKARWA+DA++LWERMR N WPMDFILYNTLL+MCAD
Sbjct: 356 FEEMVDSGLTPNEKTITAMAKIYGKARWAKDAIELWERMRLNNWPMDFILYNTLLSMCAD 415

Query: 363 LGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEINVM 422
           LGLEEEAE LFE+MK SKH RPDSWSYTAMLNIYGSGGN  ++MELFEEM  LG+++NVM
Sbjct: 416 LGLEEEAERLFEDMKGSKHCRPDSWSYTAMLNIYGSGGNAIKAMELFEEMSGLGIDLNVM 475

Query: 423 CCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFTCL 482
            CTCLIQCLGK+ RIDDLV+VF +SV++G+K DDRLCGCLLSV+SLC  S D +KV TCL
Sbjct: 476 GCTCLIQCLGKAKRIDDLVKVFTISVERGVKTDDRLCGCLLSVVSLCEESGDADKVITCL 535

Query: 483 QQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRERA 542
           QQANPKLV+F+ L+++   +F+ VK +F+ ++  TA EARRPFCNCLIDICR +NL  RA
Sbjct: 536 QQANPKLVAFVKLIEEEKTSFDTVKEDFKAVVSNTAVEARRPFCNCLIDICRKRNLYARA 595

Query: 543 HELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPEL 602
           HELLYLG+LYGLYP LHNKT  EW LDVRSLS+GAA TALEEWM TL+K VQR EALP+L
Sbjct: 596 HELLYLGTLYGLYPDLHNKTIDEWSLDVRSLSIGAAHTALEEWMETLTKFVQRNEALPKL 655

Query: 603 LSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 661
            SA TG GTH+FSQGLAN+FASHVDKLAAPF   E+RAG FVATREDLVTWV SR PS
Sbjct: 656 FSAHTGTGTHKFSQGLANAFASHVDKLAAPFTKSEERAGCFVATREDLVTWVQSRSPS 712

BLAST of Cucsa.321700 vs. TAIR10
Match: AT5G46580.1 (AT5G46580.1 pentatricopeptide (PPR) repeat-containing protein)

HSP 1 Score: 975.7 bits (2521), Expect = 1.4e-284
Identity = 463/664 (69.73%), Postives = 566/664 (85.24%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           P ++  + +  K PSLSEQLK LS TTL     ++T++LSKPKS WVNPT+PKRSVLSLQ
Sbjct: 50  PKTLEEEPITTKTPSLSEQLKPLSATTLR---QEQTQILSKPKSVWVNPTRPKRSVLSLQ 109

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           RQKRS+YSYNP+++DL++FA KLN+   ++ + F++ L+EIPHPP ++NALL+LNSLR W
Sbjct: 110 RQKRSAYSYNPQIKDLRAFALKLNSSIFTEKSEFLSLLDEIPHPPNRDNALLVLNSLREW 169

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
           QKTH FFNW+KS++LFPMETIFYNV MKSLR+GRQFQLIE++A EM+  G+ELDNITYST
Sbjct: 170 QKTHTFFNWVKSKSLFPMETIFYNVTMKSLRFGRQFQLIEEMALEMVKDGVELDNITYST 229

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAK+C+ ++KA+EWFERMYKTGLMPDEVTYSAILDVY+  GKVEEVLSLYER  A+G
Sbjct: 230 IITCAKRCNLYNKAIEWFERMYKTGLMPDEVTYSAILDVYSKSGKVEEVLSLYERAVATG 289

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           W PD   FSVLGKMFGEAGDYDGI YVLQEMKS++++PN+VVYNTLL+AMG+AGKPG AR
Sbjct: 290 WKPDAIAFSVLGKMFGEAGDYDGIRYVLQEMKSMDVKPNVVVYNTLLEAMGRAGKPGLAR 349

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLF+EM+E+G+TPNEKTLTALVKIYGKARWARDAL LWE M++  WPMDFILYNTLLNMC
Sbjct: 350 SLFNEMLEAGLTPNEKTLTALVKIYGKARWARDALQLWEEMKAKKWPMDFILYNTLLNMC 409

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           AD+GLEEEAE LF +MK+S   RPD++SYTAMLNIYGSGG  +++MELFEEML+ GV++N
Sbjct: 410 ADIGLEEEAERLFNDMKESVQCRPDNFSYTAMLNIYGSGGKAEKAMELFEEMLKAGVQVN 469

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VM CTCL+QCLGK+ RIDD+V VF++S+++G+KPDDRLCGCLLSV++LC +SED  KV  
Sbjct: 470 VMGCTCLVQCLGKAKRIDDVVYVFDLSIKRGVKPDDRLCGCLLSVMALCESSEDAEKVMA 529

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CL++AN KLV+F+NL+      +E VK EF+ ++  T  EARRPFCNCLIDICR  N  E
Sbjct: 530 CLERANKKLVTFVNLIVDEKTEYETVKEEFKLVINATQVEARRPFCNCLIDICRGNNRHE 589

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLG+L+GLYPGLHNKT  EW LDVRSLSVGAA+TALEEWM TL+ I++R+E LP
Sbjct: 590 RAHELLYLGTLFGLYPGLHNKTIKEWSLDVRSLSVGAAETALEEWMRTLANIIKRQEELP 649

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           EL  AQTG GTHRFSQGLANSFA H+ +L+APF+ + DR G FVAT+EDLV+W+ S+ P 
Sbjct: 650 ELFLAQTGTGTHRFSQGLANSFALHLQQLSAPFR-QSDRPGIFVATKEDLVSWLESKFPP 709

Query: 661 VAAT 665
           +  +
Sbjct: 710 LVTS 709

BLAST of Cucsa.321700 vs. TAIR10
Match: AT4G16390.1 (AT4G16390.1 pentatricopeptide (PPR) repeat-containing protein)

HSP 1 Score: 442.2 bits (1136), Expect = 5.7e-124
Identity = 236/615 (38.37%), Postives = 365/615 (59.35%), Query Frame = 1

Query: 46  WVNPTKPKRSVLSLQRQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPP 105
           WVNP  P+ S L   R+K    SY+ +   L   A  L+AC   ++A     +       
Sbjct: 89  WVNPKSPRASQL---RRK----SYDSRYSSLIKLAESLDAC-KPNEADVCDVITGFGGKL 148

Query: 106 TKENALLILNSLRPWQKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANE 165
            +++A++ LN++   +   L  N +        E I YNV MK  R  +  +  E L +E
Sbjct: 149 FEQDAVVTLNNMTNPETAPLVLNNLLETMKPSREVILYNVTMKVFRKSKDLEKSEKLFDE 208

Query: 166 MISAGIELDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGK 225
           M+  GI+ DN T++TII+CA++     +A+EWFE+M   G  PD VT +A++D Y   G 
Sbjct: 209 MLERGIKPDNATFTTIISCARQNGVPKRAVEWFEKMSSFGCEPDNVTMAAMIDAYGRAGN 268

Query: 226 VEEVLSLYERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNT 285
           V+  LSLY+R R   W  D  TFS L +++G +G+YDG + + +EMK++ ++PNLV+YN 
Sbjct: 269 VDMALSLYDRARTEKWRIDAVTFSTLIRIYGVSGNYDGCLNIYEEMKALGVKPNLVIYNR 328

Query: 286 LLDAMGKAGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNG 345
           L+D+MG+A +P  A+ ++ +++ +G TPN  T  ALV+ YG+AR+  DAL ++  M+  G
Sbjct: 329 LIDSMGRAKRPWQAKIIYKDLITNGFTPNWSTYAALVRAYGRARYGDDALAIYREMKEKG 388

Query: 346 WPMDFILYNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRS 405
             +  ILYNTLL+MCAD    +EA  +F++MK  +   PDSW++++++ +Y   G V  +
Sbjct: 389 LSLTVILYNTLLSMCADNRYVDEAFEIFQDMKNCETCDPDSWTFSSLITVYACSGRVSEA 448

Query: 406 MELFEEMLELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSV 465
                +M E G E  +   T +IQC GK+ ++DD+VR F+  ++ GI PDDR CGCLL+V
Sbjct: 449 EAALLQMREAGFEPTLFVLTSVIQCYGKAKQVDDVVRTFDQVLELGITPDDRFCGCLLNV 508

Query: 466 LSLCYNSEDINKVFTCLQQANPKLVSFINLL-QQNDITFEVVKNEFRNILGETAPEARRP 525
           ++    SE+I K+  C+++A PKL   + +L ++ +    V K E   ++     + ++ 
Sbjct: 509 MTQT-PSEEIGKLIGCVEKAKPKLGQVVKMLVEEQNCEEGVFKKEASELIDSIGSDVKKA 568

Query: 526 FCNCLIDICRNQNLRERAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEE 585
           + NCLID+C N N  ERA E+L LG  Y +Y GL +K+ T+W L ++SLS+GAA TAL  
Sbjct: 569 YLNCLIDLCVNLNKLERACEILQLGLEYDIYTGLQSKSATQWSLHLKSLSLGAALTALHV 628

Query: 586 WMITLSK-IVQREEALPELLSAQTGAGTHRFS-QGLANSFASHVDKLAAPFQLREDRAGW 645
           WM  LS+  ++  E  P LL   TG G H++S +GLA  F SH+ +L APF    D+ GW
Sbjct: 629 WMNDLSEAALESGEEFPPLLGINTGHGKHKYSDKGLAAVFESHLKELNAPFHEAPDKVGW 688

Query: 646 FVATREDLVTWVHSR 658
           F+ T      W+ SR
Sbjct: 689 FLTTSVAAKAWLESR 694

BLAST of Cucsa.321700 vs. TAIR10
Match: AT1G18900.3 (AT1G18900.3 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 171.0 bits (432), Expect = 2.4e-42
Identity = 116/492 (23.58%), Postives = 229/492 (46.54%), Query Frame = 1

Query: 170 GIELDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEV 229
           G + D  TY+T++    +  +F    +  + M + G  P+ VTY+ ++  Y     + E 
Sbjct: 359 GFKHDGHTYTTMVGNLGRAKQFGAINKLLDEMVRDGCQPNTVTYNRLIHSYGRANYLNEA 418

Query: 230 LSLYERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDA 289
           ++++ + + +G  PD  T+  L  +  +AG  D  M + Q M++  + P+   Y+ +++ 
Sbjct: 419 MNVFNQMQEAGCKPDRVTYCTLIDIHAKAGFLDIAMDMYQRMQAGGLSPDTFTYSVIINC 478

Query: 290 MGKAGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMD 349
           +GKAG    A  LF EMV+ G TPN  T   ++ ++ KAR  ++AL L+  M++ G+  D
Sbjct: 479 LGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMMDLHAKARNYQNALKLYRDMQNAGFEPD 538

Query: 350 FILYNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELF 409
            + Y+ ++ +    G  EEAE +F EM++ K+  PD   Y  +++++G  GNV+++ + +
Sbjct: 539 KVTYSIVMEVLGHCGYLEEAEAVFTEMQQ-KNWIPDEPVYGLLVDLWGKAGNVEKAWQWY 598

Query: 410 EEMLELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLC 469
           + ML  G+  NV  C  L+    +  +I +   +    +  G++P  +    LLS  +  
Sbjct: 599 QAMLHAGLRPNVPTCNSLLSTFLRVNKIAEAYELLQNMLALGLRPSLQTYTLLLSCCTDG 658

Query: 470 YNSEDINKVFTCLQQANPKLVSFINLLQQNDITFEVVK---NEFRNILGETAPEARRPFC 529
            +  D+      +         F+  +       E V+   N F +++     E++R   
Sbjct: 659 RSKLDMGFCGQLMASTGHPAHMFLLKMPAAGPDGENVRNHANNFLDLMHSEDRESKRGLV 718

Query: 530 NCLIDICRNQNLRERAHELLYLGSLYGLYP-GLHNKTETEWCLDVRSLSVGAAQTALEEW 589
           + ++D       +E A  +  + +   ++P  L  K+ + W +++  +S G A TAL   
Sbjct: 719 DAVVDFLHKSGQKEEAGSVWEVAAQKNVFPDALREKSCSYWLINLHVMSEGTAVTALSRT 778

Query: 590 MITLSKIVQREEALPELLSAQTGAGTHRFSQG---LANSFASHVDKLAAPFQLREDRAGW 649
           +    K +      P  +   TG G      G   +  +    ++   +PF      +G 
Sbjct: 779 LAWFRKQMLASGTCPSRIDIVTGWGRRSRVTGTSMVRQAVEELLNIFGSPFFTESGNSGC 838

Query: 650 FVATREDLVTWV 655
           FV + E L  W+
Sbjct: 839 FVGSGEPLNRWL 849

HSP 2 Score: 163.3 bits (412), Expect = 5.1e-40
Identity = 125/546 (22.89%), Postives = 236/546 (43.22%), Query Frame = 1

Query: 113 ILNSLRPWQKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIE 172
           +L  +  +     FF W+K Q  F  +   Y   + +L   +QF  I  L +EM+  G +
Sbjct: 337 VLKQMNDYGNALGFFYWLKRQPGFKHDGHTYTTMVGNLGRAKQFGAINKLLDEMVRDGCQ 396

Query: 173 LDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSL 232
            + +TY+ +I    + +  ++AM  F +M + G  PD VTY  ++D++A  G ++  + +
Sbjct: 397 PNTVTYNRLIHSYGRANYLNEAMNVFNQMQEAGCKPDRVTYCTLIDIHAKAGFLDIAMDM 456

Query: 233 YERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGK 292
           Y+R +A G +PD +T+SV+    G+AG       +  EM      PNLV YN ++D   K
Sbjct: 457 YQRMQAGGLSPDTFTYSVIINCLGKAGHLPAAHKLFCEMVDQGCTPNLVTYNIMMDLHAK 516

Query: 293 AGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFIL 352
           A     A  L+ +M  +G  P++ T + ++++ G   +  +A  ++  M+   W  D  +
Sbjct: 517 ARNYQNALKLYRDMQNAGFEPDKVTYSIVMEVLGHCGYLEEAEAVFTEMQQKNWIPDEPV 576

Query: 353 YNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEM 412
           Y  L+++    G  E+A   ++ M  +   RP+  +  ++L+ +     +  + EL + M
Sbjct: 577 YGLLVDLWGKAGNVEKAWQWYQAMLHA-GLRPNVPTCNSLLSTFLRVNKIAEAYELLQNM 636

Query: 413 LELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNS 472
           L LG+  ++   T L+ C    GR                K D   CG L++        
Sbjct: 637 LALGLRPSLQTYTLLLSCC-TDGR---------------SKLDMGFCGQLMA-----STG 696

Query: 473 EDINKVFTCLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDI 532
              +     +  A P   +  N             N F +++     E++R   + ++D 
Sbjct: 697 HPAHMFLLKMPAAGPDGENVRN-----------HANNFLDLMHSEDRESKRGLVDAVVDF 756

Query: 533 CRNQNLRERAHELLYLGSLYGLYP-GLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSK 592
                 +E A  +  + +   ++P  L  K+ + W +++  +S G A TAL   +    K
Sbjct: 757 LHKSGQKEEAGSVWEVAAQKNVFPDALREKSCSYWLINLHVMSEGTAVTALSRTLAWFRK 816

Query: 593 IVQREEALPELLSAQTGAGTHRFSQG---LANSFASHVDKLAAPFQLREDRAGWFVATRE 652
            +      P  +   TG G      G   +  +    ++   +PF      +G FV + E
Sbjct: 817 QMLASGTCPSRIDIVTGWGRRSRVTGTSMVRQAVEELLNIFGSPFFTESGNSGCFVGSGE 849

Query: 653 DLVTWV 655
            L  W+
Sbjct: 877 PLNRWL 849

BLAST of Cucsa.321700 vs. TAIR10
Match: AT1G74750.1 (AT1G74750.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 167.2 bits (422), Expect = 3.5e-41
Identity = 116/492 (23.58%), Postives = 225/492 (45.73%), Query Frame = 1

Query: 170 GIELDNITYSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEV 229
           G + D  TY+T++    +  +F +  +  + M + G  P+ VTY+ ++  Y     ++E 
Sbjct: 354 GFKHDGHTYTTMVGNLGRAKQFGEINKLLDEMVRDGCKPNTVTYNRLIHSYGRANYLKEA 413

Query: 230 LSLYERGRASGWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDA 289
           ++++ + + +G  PD  T+  L  +  +AG  D  M + Q M+   + P+   Y+ +++ 
Sbjct: 414 MNVFNQMQEAGCEPDRVTYCTLIDIHAKAGFLDIAMDMYQRMQEAGLSPDTFTYSVIINC 473

Query: 290 MGKAGKPGFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMD 349
           +GKAG    A  LF EMV  G TPN  T   ++ ++ KAR    AL L+  M++ G+  D
Sbjct: 474 LGKAGHLPAAHRLFCEMVGQGCTPNLVTFNIMIALHAKARNYETALKLYRDMQNAGFQPD 533

Query: 350 FILYNTLLNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELF 409
            + Y+ ++ +    G  EEAE +F EM++ K+  PD   Y  +++++G  GNV ++ + +
Sbjct: 534 KVTYSIVMEVLGHCGFLEEAEGVFAEMQR-KNWVPDEPVYGLLVDLWGKAGNVDKAWQWY 593

Query: 410 EEMLELGVEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLC 469
           + ML+ G+  NV  C  L+    +  R+ +   +    +  G+ P  +    LLS  +  
Sbjct: 594 QAMLQAGLRPNVPTCNSLLSTFLRVHRMSEAYNLLQSMLALGLHPSLQTYTLLLSCCTDA 653

Query: 470 YNSEDINKVFTCLQQANPKLVSFINLLQQNDITFEVVKNEFRNILG---ETAPEARRPFC 529
            ++ D+      +  +      F+  +       + V++   N L        E++R   
Sbjct: 654 RSNFDMGFCGQLMAVSGHPAHMFLLKMPPAGPDGQKVRDHVSNFLDFMHSEDRESKRGLM 713

Query: 530 NCLIDICRNQNLRERAHELLYLGSLYGLYP-GLHNKTETEWCLDVRSLSVGAAQTALEEW 589
           + ++D      L+E A  +  + +   +YP  L  K+ + W +++  +S G A  AL   
Sbjct: 714 DAVVDFLHKSGLKEEAGSVWEVAAGKNVYPDALREKSYSYWLINLHVMSEGTAVIALSRT 773

Query: 590 MITLSKIVQREEALPELLSAQTGAGTHRFSQG---LANSFASHVDKLAAPFQLREDRAGW 649
           +    K +      P  +   TG G      G   +  +    ++    PF      +G 
Sbjct: 774 LAWFRKQMLVSGDCPSRIDIVTGWGRRSRVTGTSMVRQAVEELLNIFNFPFFTENGNSGC 833

Query: 650 FVATREDLVTWV 655
           FV + E L  W+
Sbjct: 834 FVGSGEPLKNWL 844

BLAST of Cucsa.321700 vs. TAIR10
Match: AT1G74850.1 (AT1G74850.1 plastid transcriptionally active 2)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-39
Identity = 134/586 (22.87%), Postives = 245/586 (41.81%), Query Frame = 1

Query: 120 WQKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYS 179
           W+     F  ++ + + P + + YN  + +          E +   M   GI  D  TYS
Sbjct: 228 WEGLLGLFAEMRHEGIQP-DIVTYNTLLSACAIRGLGDEAEMVFRTMNDGGIVPDLTTYS 287

Query: 180 TIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRAS 239
            ++    K  R +K  +    M   G +PD  +Y+ +L+ YA  G ++E + ++ + +A+
Sbjct: 288 HLVETFGKLRRLEKVCDLLGEMASGGSLPDITSYNVLLEAYAKSGSIKEAMGVFHQMQAA 347

Query: 240 GWTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFA 299
           G TP+  T+SVL  +FG++G YD +  +  EMKS    P+   YN L++  G+ G     
Sbjct: 348 GCTPNANTYSVLLNLFGQSGRYDDVRQLFLEMKSSNTDPDAATYNILIEVFGEGGYFKEV 407

Query: 300 RSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNM 359
            +LF +MVE  I P+ +T   ++   GK     DA  + + M +N        Y  ++  
Sbjct: 408 VTLFHDMVEENIEPDMETYEGIIFACGKGGLHEDARKILQYMTANDIVPSSKAYTGVIEA 467

Query: 360 CADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEI 419
                L EEA   F  M +   S P   ++ ++L  +  GG VK S  +   +++ G+  
Sbjct: 468 FGQAALYEEALVAFNTMHE-VGSNPSIETFHSLLYSFARGGLVKESEAILSRLVDSGIPR 527

Query: 420 NVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVF 479
           N       I+   + G+ ++ V+ +    +    PD+R    +LSV S     ++  + F
Sbjct: 528 NRDTFNAQIEAYKQGGKFEEAVKTYVDMEKSRCDPDERTLEAVLSVYSFARLVDECREQF 587

Query: 480 TCLQQAN--PKLVSFINLL----------QQNDITFEVVKNEFRNI-------------- 539
             ++ ++  P ++ +  +L            N++  E++ N   NI              
Sbjct: 588 EEMKASDILPSIMCYCMMLAVYGKTERWDDVNELLEEMLSNRVSNIHQVIGQMIKGDYDD 647

Query: 540 -------------LGETAPEARRPFCNCLIDICRNQNLRERAHELLYLGSLYGLYPGLHN 599
                        L          F N L+D       +ERA  +L   +  GL+P L  
Sbjct: 648 DSNWQIVEYVLDKLNSEGCGLGIRFYNALLDALWWLGQKERAARVLNEATKRGLFPELFR 707

Query: 600 KTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPELLSAQTGAGTHRFSQGLAN 659
           K +  W +DV  +S G   TAL  W+  ++ ++ + + LP+L    +  G    S     
Sbjct: 708 KNKLVWSVDVHRMSEGGMYTALSVWLNDINDMLLKGD-LPQLAVVVSVRGQLEKSSAARE 767

Query: 660 S------FASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 661
           S      F+   D +++ F       G  +  R  L   + ++ P+
Sbjct: 768 SPIAKAAFSFLQDHVSSSFSFTGWNGGRIMCQRSQLKQLLSTKEPT 810

HSP 2 Score: 128.6 bits (322), Expect = 1.4e-29
Identity = 81/279 (29.03%), Postives = 139/279 (49.82%), Query Frame = 1

Query: 178 YSTIITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGR 237
           Y+ +I+   +    DK +E F+ M   G+     +Y+A+++ Y   G+ E  L L +R +
Sbjct: 144 YTIMISLLGREGLLDKCLEVFDEMPSQGVSRSVFSYTALINAYGRNGRYETSLELLDRMK 203

Query: 238 ASGWTPDPYTFSVLGKMFGEAG-DYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKP 297
               +P   T++ +       G D++G++ +  EM+   +QP++V YNTLL A    G  
Sbjct: 204 NEKISPSILTYNTVINACARGGLDWEGLLGLFAEMRHEGIQPDIVTYNTLLSACAIRGLG 263

Query: 298 GFARSLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTL 357
             A  +F  M + GI P+  T + LV+ +GK R      DL   M S G   D   YN L
Sbjct: 264 DEAEMVFRTMNDGGIVPDLTTYSHLVETFGKLRRLEKVCDLLGEMASGGSLPDITSYNVL 323

Query: 358 LNMCADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELG 417
           L   A  G  +EA  +F +M ++    P++ +Y+ +LN++G  G      +LF EM    
Sbjct: 324 LEAYAKSGSIKEAMGVFHQM-QAAGCTPNANTYSVLLNLFGQSGRYDDVRQLFLEMKSSN 383

Query: 418 VEINVMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPD 456
            + +      LI+  G+ G   ++V +F+  V++ I+PD
Sbjct: 384 TDPDAATYNILIEVFGEGGYFKEVVTLFHDMVEENIEPD 421

HSP 3 Score: 55.1 bits (131), Expect = 2.0e-07
Identity = 42/206 (20.39%), Postives = 94/206 (45.63%), Query Frame = 1

Query: 75  DLKSFAHKLNAC-------DSSDDASFIAALEEIPHPPTKENALLILNSLRPWQKTHLFF 134
           D++++   + AC       D+     ++ A + +P        +        +++  + F
Sbjct: 421 DMETYEGIIFACGKGGLHEDARKILQYMTANDIVPSSKAYTGVIEAFGQAALYEEALVAF 480

Query: 135 NWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTIITCAKK 194
           N +      P    F+++ + S   G   +  E + + ++ +GI  +  T++  I   K+
Sbjct: 481 NTMHEVGSNPSIETFHSL-LYSFARGGLVKESEAILSRLVDSGIPRNRDTFNAQIEAYKQ 540

Query: 195 CSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWTPDPYT 254
             +F++A++ +  M K+   PDE T  A+L VY+    V+E    +E  +AS   P    
Sbjct: 541 GGKFEEAVKTYVDMEKSRCDPDERTLEAVLSVYSFARLVDECREQFEEMKASDILPSIMC 600

Query: 255 FSVLGKMFGEAGDYDGIMYVLQEMKS 274
           + ++  ++G+   +D +  +L+EM S
Sbjct: 601 YCMMLAVYGKTERWDDVNELLEEMLS 625

BLAST of Cucsa.321700 vs. NCBI nr
Match: gi|449462001|ref|XP_004148730.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g46580, chloroplastic [Cucumis sativus])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 665/665 (100.00%), Postives = 665/665 (100.00%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ
Sbjct: 46  PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 105

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW
Sbjct: 106 RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 165

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
           QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST
Sbjct: 166 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 225

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG
Sbjct: 226 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 285

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR
Sbjct: 286 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 345

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC
Sbjct: 346 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 405

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN
Sbjct: 406 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 465

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT
Sbjct: 466 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 525

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE
Sbjct: 526 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 585

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP
Sbjct: 586 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 645

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS
Sbjct: 646 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 705

Query: 661 VAATA 666
           VAATA
Sbjct: 706 VAATA 710

BLAST of Cucsa.321700 vs. NCBI nr
Match: gi|659095679|ref|XP_008448710.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g46580, chloroplastic [Cucumis melo])

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 644/665 (96.84%), Postives = 657/665 (98.80%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           PSS+SS+S+DNKNPSLS+QLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ
Sbjct: 46  PSSISSESIDNKNPSLSDQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 105

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           RQKRSSYSYNPKMRDLKSFAHKLNACDSSD+ASFIAALEEIPHPPTKENALLILNSLRPW
Sbjct: 106 RQKRSSYSYNPKMRDLKSFAHKLNACDSSDEASFIAALEEIPHPPTKENALLILNSLRPW 165

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
           QKTHLFFNWIK+QNLFPMETIFYNVAMKSLRYGRQFQLIEDLAN+M+S GIELDNITYST
Sbjct: 166 QKTHLFFNWIKTQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANDMVSTGIELDNITYST 225

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG
Sbjct: 226 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 285

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           W PDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR
Sbjct: 286 WKPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 345

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC
Sbjct: 346 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 405

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           ADLGLEEEAE LFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEML+LGVEIN
Sbjct: 406 ADLGLEEEAEKLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLKLGVEIN 465

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSV+SLC NSEDINKVFT
Sbjct: 466 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVVSLCDNSEDINKVFT 525

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CLQQANPKLVSF+NLLQQN ITFEV+KNEFRNIL ETA EARRPFCNCLIDICRNQNLRE
Sbjct: 526 CLQQANPKLVSFVNLLQQNSITFEVIKNEFRNILSETASEARRPFCNCLIDICRNQNLRE 585

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQR+EALP
Sbjct: 586 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQRKEALP 645

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS
Sbjct: 646 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 705

Query: 661 VAATA 666
           V ATA
Sbjct: 706 VPATA 710

BLAST of Cucsa.321700 vs. NCBI nr
Match: gi|225427240|ref|XP_002278451.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g46580, chloroplastic [Vitis vinifera])

HSP 1 Score: 1067.8 bits (2760), Expect = 7.9e-309
Identity = 516/663 (77.83%), Postives = 590/663 (88.99%), Query Frame = 1

Query: 1   PSSVSSQSVDNKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQ 60
           P+S  S+  +++NPSLSEQLK LS T L+   + +T L+SKPKSTW+NPTKPK SVLSLQ
Sbjct: 60  PTSSDSEQTNHQNPSLSEQLKPLSKTILTRDHSGQTHLVSKPKSTWINPTKPKPSVLSLQ 119

Query: 61  RQKRSSYSYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPW 120
           R KR +YSYNP++RDLK FA K+N  +SSD++ F+A LE+IPHPPT++NALL+LNSL+PW
Sbjct: 120 RHKRHNYSYNPQIRDLKLFAKKINESESSDESEFLAVLEQIPHPPTRDNALLLLNSLKPW 179

Query: 121 QKTHLFFNWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYST 180
            KT+LFFNWIK+QNLFPMETIFYNV MKSLR+GRQFQLIE+LANEMIS G+ELDNITYST
Sbjct: 180 PKTYLFFNWIKTQNLFPMETIFYNVTMKSLRFGRQFQLIEELANEMISTGVELDNITYST 239

Query: 181 IITCAKKCSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASG 240
           IITCAK+C+ FDKA++WFERMYKTGLMPDEVTYSAILDVYA LGKVEEVLSLYERGRASG
Sbjct: 240 IITCAKRCNLFDKAVKWFERMYKTGLMPDEVTYSAILDVYAKLGKVEEVLSLYERGRASG 299

Query: 241 WTPDPYTFSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFAR 300
           W PDP  F+VLGKMFGEAGDYDGI YVLQEMKS+ +QPNLVVYNTLL+AMGKAGKPG AR
Sbjct: 300 WKPDPIAFAVLGKMFGEAGDYDGIRYVLQEMKSLGVQPNLVVYNTLLEAMGKAGKPGLAR 359

Query: 301 SLFDEMVESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMC 360
           SLF+EMV SG+ P+ KTLTALVKIYGKARWARDAL+LWERMRSNGWPMDFILYNTLL+MC
Sbjct: 360 SLFEEMVGSGVIPDAKTLTALVKIYGKARWARDALELWERMRSNGWPMDFILYNTLLSMC 419

Query: 361 ADLGLEEEAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEIN 420
           ADLGLEEEAE LFE+MKKS+H RPDSWSYTAMLNIYGSGGNV R+M+LF+EM ELGV+IN
Sbjct: 420 ADLGLEEEAEKLFEDMKKSEHCRPDSWSYTAMLNIYGSGGNVDRAMQLFDEMSELGVQIN 479

Query: 421 VMCCTCLIQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFT 480
           VM CTCL QCLG++ RIDDLV+VF VS+++G+KPDDRLCGCLLSV+S C  +ED NKV  
Sbjct: 480 VMGCTCLSQCLGRARRIDDLVKVFEVSLERGVKPDDRLCGCLLSVVSFCEGAEDANKVLA 539

Query: 481 CLQQANPKLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRE 540
           CLQQANPKLV+F+NLL++  I+FE +K EFR IL +TA EARRPFCNCLIDICRN++L E
Sbjct: 540 CLQQANPKLVAFVNLLEEK-ISFEALKEEFRGILTDTAVEARRPFCNCLIDICRNRSLHE 599

Query: 541 RAHELLYLGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALP 600
           RAHELLYLG+LYGLYPGLHN+T  EWCLDVRSLSVGAA TALEEWM TLSKIVQREEALP
Sbjct: 600 RAHELLYLGTLYGLYPGLHNRTADEWCLDVRSLSVGAAHTALEEWMGTLSKIVQREEALP 659

Query: 601 ELLSAQTGAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPS 660
           E  SA TG GTH+FSQGLA++FASHV KLAAPF   E++AG FVATREDLV+WV SR+ S
Sbjct: 660 EAFSANTGTGTHKFSQGLASAFASHVKKLAAPFTQSEEKAGCFVATREDLVSWVQSRILS 719

Query: 661 VAA 664
            AA
Sbjct: 720 PAA 721

BLAST of Cucsa.321700 vs. NCBI nr
Match: gi|595852519|ref|XP_007210329.1| (hypothetical protein PRUPE_ppa002049mg [Prunus persica])

HSP 1 Score: 1060.8 bits (2742), Expect = 9.6e-307
Identity = 514/658 (78.12%), Postives = 585/658 (88.91%), Query Frame = 1

Query: 10  DNKNPSLS--EQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQRQKRSSY 69
           + KNPSLS  EQL+ L++TTLSN P D+++LLSKPKS WVNP KPKRSVLSLQRQKRS Y
Sbjct: 68  NKKNPSLSLSEQLQPLTSTTLSNPPKDQSQLLSKPKSIWVNPAKPKRSVLSLQRQKRSLY 127

Query: 70  SYNPKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPWQKTHLFF 129
           SYNP++RDL+ FAHKLN CD+S +A F+AALEEIPHPPT+ENALLILNSL+PWQKTH+FF
Sbjct: 128 SYNPQVRDLRQFAHKLNDCDASQNA-FLAALEEIPHPPTRENALLILNSLKPWQKTHMFF 187

Query: 130 NWIKSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTIITCAKK 189
           NW+K+QN FPM+TIFYNV MKSLR+GRQFQLIE+LA EM+S  IELDNITYSTIITCAK+
Sbjct: 188 NWVKAQNSFPMDTIFYNVTMKSLRFGRQFQLIEELAEEMVSNEIELDNITYSTIITCAKR 247

Query: 190 CSRFDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWTPDPYT 249
              FDKA+EWFERMYKTGLMPDEVTYSAILDVYA LGKVEEVLSLYERGRASGW PDP  
Sbjct: 248 SKLFDKAVEWFERMYKTGLMPDEVTYSAILDVYAKLGKVEEVLSLYERGRASGWKPDPIA 307

Query: 250 FSVLGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFARSLFDEMV 309
           FSVLGKMFGEAGDYDGI YVLQEM ++ +QPNLVVYNTLL+AMGKAGKPG ARSLF+EMV
Sbjct: 308 FSVLGKMFGEAGDYDGIRYVLQEMAALGVQPNLVVYNTLLEAMGKAGKPGLARSLFEEMV 367

Query: 310 ESGITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMCADLGLEE 369
            SG+ PNEKTLTALVKIYGKARWARDAL+LWERMRSN WPMDFILYNTLLNMCADLGLEE
Sbjct: 368 GSGLKPNEKTLTALVKIYGKARWARDALELWERMRSNEWPMDFILYNTLLNMCADLGLEE 427

Query: 370 EAETLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEINVMCCTCL 429
           EA+ LFE+MK+S+H RPDSWSYTAMLNI+GSGGNV  +M LFEEM ELG+E+NVM CTCL
Sbjct: 428 EAKKLFEDMKQSEHCRPDSWSYTAMLNIFGSGGNVDGAMGLFEEMSELGIELNVMGCTCL 487

Query: 430 IQCLGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFTCLQQANP 489
           IQCLGK+ R  D+VRVF V+V++G+KPDDRLCGCLLSV+SLC  +ED +KV +CLQQANP
Sbjct: 488 IQCLGKARRFSDMVRVFGVAVERGVKPDDRLCGCLLSVVSLCEKTEDEDKVLSCLQQANP 547

Query: 490 KLVSFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRERAHELLY 549
           KLV+ + +LQ   + FE +K+EFR+++  T+ E+RRPFCNCLIDICRN+N  ERAHELLY
Sbjct: 548 KLVTLVKVLQDKKLGFETIKDEFRDVISGTSVESRRPFCNCLIDICRNKNNHERAHELLY 607

Query: 550 LGSLYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPELLSAQT 609
           LG+LYGLYPGLHNKT  EWCLDVRSLS+GAA TALEEWM TL KIVQREEALPEL SAQT
Sbjct: 608 LGTLYGLYPGLHNKTSREWCLDVRSLSIGAAHTALEEWMGTLYKIVQREEALPELFSAQT 667

Query: 610 GAGTHRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPSVAATA 666
           G GTH+FSQGLA+SFASHV+KLAAPF+  E++AG FVATREDLV+WV S+ PS A TA
Sbjct: 668 GTGTHKFSQGLAHSFASHVEKLAAPFRKSEEKAGRFVATREDLVSWVQSQAPSTAITA 724

BLAST of Cucsa.321700 vs. NCBI nr
Match: gi|645265732|ref|XP_008238288.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g46580, chloroplastic [Prunus mume])

HSP 1 Score: 1053.9 bits (2724), Expect = 1.2e-304
Identity = 509/655 (77.71%), Postives = 581/655 (88.70%), Query Frame = 1

Query: 11  NKNPSLSEQLKNLSTTTLSNAPNDETRLLSKPKSTWVNPTKPKRSVLSLQRQKRSSYSYN 70
           N + SLSEQL+ L++TTLSN P ++++LLSKPKS WVNP KPKRSVLSLQRQKRS YSYN
Sbjct: 70  NSSLSLSEQLQPLTSTTLSNPPKEQSQLLSKPKSIWVNPAKPKRSVLSLQRQKRSLYSYN 129

Query: 71  PKMRDLKSFAHKLNACDSSDDASFIAALEEIPHPPTKENALLILNSLRPWQKTHLFFNWI 130
           P++RDL+ FAHKLN CD+S  A F+AALEEIPHPPT+ENALLILNSL+PWQKTH+FFNW+
Sbjct: 130 PQVRDLRQFAHKLNDCDASQSA-FLAALEEIPHPPTRENALLILNSLKPWQKTHMFFNWV 189

Query: 131 KSQNLFPMETIFYNVAMKSLRYGRQFQLIEDLANEMISAGIELDNITYSTIITCAKKCSR 190
           K+QN FPM+TIFYNV MKSLR+GRQFQLIE+LA EM+S  IELDNITYSTIITCAK+   
Sbjct: 190 KAQNSFPMDTIFYNVTMKSLRFGRQFQLIEELAEEMVSNEIELDNITYSTIITCAKRSKL 249

Query: 191 FDKAMEWFERMYKTGLMPDEVTYSAILDVYANLGKVEEVLSLYERGRASGWTPDPYTFSV 250
           FDKA+EWFERMYKTGLMPDEVTYSAILDVYA LGKVEEVLSLYERGRASGW PDP  FSV
Sbjct: 250 FDKAVEWFERMYKTGLMPDEVTYSAILDVYAKLGKVEEVLSLYERGRASGWKPDPIAFSV 309

Query: 251 LGKMFGEAGDYDGIMYVLQEMKSIEMQPNLVVYNTLLDAMGKAGKPGFARSLFDEMVESG 310
           LGKMFGEAGDYDGI YVLQEM ++ +QPNLVVYNTLL+AMGKAGKPG ARSLF+EMV SG
Sbjct: 310 LGKMFGEAGDYDGIRYVLQEMAALGVQPNLVVYNTLLEAMGKAGKPGLARSLFEEMVGSG 369

Query: 311 ITPNEKTLTALVKIYGKARWARDALDLWERMRSNGWPMDFILYNTLLNMCADLGLEEEAE 370
           + PNEKTLTALVKIYGKARWARDAL+LWERMRSN WPMDFILYNTLLNMCADLGLEEEA+
Sbjct: 370 LKPNEKTLTALVKIYGKARWARDALELWERMRSNEWPMDFILYNTLLNMCADLGLEEEAK 429

Query: 371 TLFEEMKKSKHSRPDSWSYTAMLNIYGSGGNVKRSMELFEEMLELGVEINVMCCTCLIQC 430
            LF +MK+S+H RPDSWSYTAMLNI+GSGGNV  +M LFEEM ELG+E+NVM CTCLIQC
Sbjct: 430 KLFGDMKQSEHCRPDSWSYTAMLNIFGSGGNVDEAMGLFEEMSELGIELNVMGCTCLIQC 489

Query: 431 LGKSGRIDDLVRVFNVSVQKGIKPDDRLCGCLLSVLSLCYNSEDINKVFTCLQQANPKLV 490
           LGK+ R  D+VRVF V+V++G+KPDDRLCGCLLSV+SLC  +ED +KV +CLQQANPKLV
Sbjct: 490 LGKARRFGDMVRVFGVAVERGVKPDDRLCGCLLSVVSLCEKTEDEDKVLSCLQQANPKLV 549

Query: 491 SFINLLQQNDITFEVVKNEFRNILGETAPEARRPFCNCLIDICRNQNLRERAHELLYLGS 550
           + + +LQ   + FE +K+EFR+++  T+ E+RRPFCNCLIDICRN++  ERAHELLYLG+
Sbjct: 550 TLVKVLQDKKLGFETIKDEFRDVISGTSVESRRPFCNCLIDICRNKSNHERAHELLYLGT 609

Query: 551 LYGLYPGLHNKTETEWCLDVRSLSVGAAQTALEEWMITLSKIVQREEALPELLSAQTGAG 610
           LYGLYPGLHNKT  EWCLDVRSLS+GAA TALEEWM TL KIVQREEALPEL SAQTG G
Sbjct: 610 LYGLYPGLHNKTSKEWCLDVRSLSIGAAHTALEEWMGTLYKIVQREEALPELFSAQTGTG 669

Query: 611 THRFSQGLANSFASHVDKLAAPFQLREDRAGWFVATREDLVTWVHSRVPSVAATA 666
           TH+FSQGLA+SFASHV+KLAAPF+  E++AG FVATREDLV+WV S+ PS A TA
Sbjct: 670 THKFSQGLAHSFASHVEKLAAPFRKSEEKAGRFVATREDLVSWVQSQAPSTAITA 723