Cucsa.308540 (gene) Cucumber (Gy14) v1

NameCucsa.308540
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionThioredoxin
Locationscaffold02978 : 611480 .. 613565 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTTTGAATTTTCTTTTGTTTCCCAATTCTTCTCCCCTTCCGAAATGGTCAAAGCTCTGCCCCTTCACCACGGAATTGTGAAATCTTTTCCCAAATTCGGCAGTTCTTTTGATCTTCCTCCGTCTCCTCAACTTCTGTCCGCGAGGCTCGCCGGCTCTGGTTTCAATAGGCTTCCTTTGTCTCGGAAATTTCCGCCGCTTTTTCAGAAAGTTTCTGCGACTGGTGAGAAGACTTCGCTTTTTGGAGCTTGGACCCAAGATAGTGCCTTGCAATTTCGAGATGAATCGCCTGTTTCGATTGAGCTTAAGTCAATTTCGAGTGAACTTGAGTTCGATAAGGCTATTGCAGATGCGGAGGAACGCAACGAGCTCGTGATTCTTGTTTGGTATGTTTAAGTTTCTTGTGGGATTTTTCTGGGAAATGTTGAGTTAATTACGGAACTTTCTAACAAAGCGTTTTTCGGCTTAAGTTTTAGAGAGACGATGTGAAAGTATTTGAGTTTGACTGAATCTTGTTTGATTAGAAGTTTATTTGTTTATTAGGAAAGATTGTTCTCAACTGCTAGATTGATTAACCTTCGTTGTACATTGATTTTGATCATTTGGAAGTGAATGGCGTGATTGATATTCTTCTTCAATTTTCCTTACAAGCCTTTCAAATGCATTTCTCATGATTTTATCATCTTTAGTTGAAAAGTGAATTTATGTGGATCTGTAGGATGGCTAACTGGTGCAGAAATTATATCTATTTAAAGCCTCAACTGGAAAGATTGGCTGCTGATTATTACCCCAGGTTAGCTCCTAACCCATTTCTTCTAATCAACCAAATGGCTAAAATTGTACTATATTTTCATTCTCCATGTGCTGGATACTTCTTAAAAGATCAGTTTTTCAGTCCTTATTGAAGTTTTGTATGTGCTTGATGAATTGTAGATTGCAATTCTACTGCATTGATGTCAATGTGGTGCCACACAAGCTAGTTGTTCGAGCCGGACTAGCTGTAAGTTCCTTTCAAAGCCTTTTGATGTTTGTTCCATTTTTTTTGTGTTGCCTTAAACCTTTATATTGATCTTCTTTAGGTAGGACTTGAGGTTATGATAACTTCTACTTCTCTGAGAGAGTGATTTTCTTTGGATACTGTACTAGAGCTTCTGTCTACAATGGAAATTAGGGCTTGTGTTTCATTGCCTTCAGGAACATTCCCAACTTGCAGTTTGAACATAGGAGACAGTAGACATGTTGAGTTTATGTTACTTTGATGGATTAGTTTATTGAATAAATTACATTATCTGTTGAGTGTTTAATTCGAATATGTCCAAACAAAAAATCTTTCCTTGGTTGAGTGATACAAATTCTGATAGTTTTGATTTTGTACATCTTCTCATGTATCTCAATCGTTCGCCTTTGAATCATCCAGAAGATGCCAGCTATACAGGTGAGTTTCCTCACAATCAGATATCTTATAGCTGGAAACATTTCTCTCTACTAACGTCAAGATCTTAATTGTGTTTATTGCAGCTATGGAAGGATGGTAAGAAACAAGATGAGGTGGTTGGTCTCTACAAAGCATATCTAGTTGTCAACGATGTTCGGAAAATGATTGAACGTGAGCTAACAGATGAGTGACATACGATTCATTTTTACCCACATCCTCTTATCCACTTAGTGGTATTGAAATTGATTGATAACATCTCACAGAAATTCCTTTCTGCTTATATTCATCAACATAAAGAACAAAGCCTGGCTGGCCTTCTTGCCAAGTCTTTGTCATTGCAGAGATCATTTCTTTGTTGTCATGGGGTGGATGATCTAACATGTATGTCCAATTCTTGACAATATACGCATTGTGAGGAAGTCTTATTTGTAAGGTTTACCTTCCTTTTTATACCATTCCTTTCCCTTATATCTGATATGCAGAGGGCTCTGTTTTGTGTTGGTAGATTAATTTAATTGTTCTGATCTATTATGAATTACACGTCTCTTTCCGAGTCTTCTGGATCTCTCTGCTTTTAACAATTAATGGATGTTGCCTTGAAAAAGCACTTGAGGAGAGGATTAGGTGGACCTTGAACATTGAAAAGAGCTA

mRNA sequence

CCCTTTGAATTTTCTTTTGTTTCCCAATTCTTCTCCCCTTCCGAAATGGTCAAAGCTCTGCCCCTTCACCACGGAATTGTGAAATCTTTTCCCAAATTCGGCAGTTCTTTTGATCTTCCTCCGTCTCCTCAACTTCTGTCCGCGAGGCTCGCCGGCTCTGGTTTCAATAGGCTTCCTTTGTCTCGGAAATTTCCGCCGCTTTTTCAGAAAGTTTCTGCGACTGGTGAGAAGACTTCGCTTTTTGGAGCTTGGACCCAAGATAGTGCCTTGCAATTTCGAGATGAATCGCCTGTTTCGATTGAGCTTAAGTCAATTTCGAGTGAACTTGAGTTCGATAAGGCTATTGCAGATGCGGAGGAACGCAACGAGCTCGTGATTCTTGTTTGGATGGCTAACTGGTGCAGAAATTATATCTATTTAAAGCCTCAACTGGAAAGATTGGCTGCTGATTATTACCCCAGATTGCAATTCTACTGCATTGATGTCAATGTGGTGCCACACAAGCTAGTTGTTCGAGCCGGACTAGCTAAGATGCCAGCTATACAGCTATGGAAGGATGGTAAGAAACAAGATGAGGTGGTTGGTCTCTACAAAGCATATCTAGTTGTCAACGATGTTCGGAAAATGATTGAACGTGAGCTAACAGATGAGTGACATACGATTCATTTTTACCCACATCCTCTTATCCACTTAGTGGTATTGAAATTGATTGATAACATCTCACAGAAATTCCTTTCTGCTTATATTCATCAACATAAAGAACAAAGCCTGGCTGGCCTTCTTGCCAAGTCTTTGTCATTGCAGAGATCATTTCTTTGTTGTCATGGGGTGGATGATCTAACATGTATGTCCAATTCTTGACAATATACGCATTGTGAGGAAGTCTTATTTGTAAGGTTTACCTTCCTTTTTATACCATTCCTTTCCCTTATATCTGATATGCAGAGGGCTCTGTTTTGTGTTGGTAGATTAATTTAATTGTTCTGATCTATTATGAATTACACGTCTCTTTCCGAGTCTTCTGGATCTCTCTGCTTTTAACAATTAATGGATGTTGCCTTGAAAAAGCACTTGAGGAGAGGATTAGGTGGACCTTGAACATTGAAAAGAGCTA

Coding sequence (CDS)

ATGGTCAAAGCTCTGCCCCTTCACCACGGAATTGTGAAATCTTTTCCCAAATTCGGCAGTTCTTTTGATCTTCCTCCGTCTCCTCAACTTCTGTCCGCGAGGCTCGCCGGCTCTGGTTTCAATAGGCTTCCTTTGTCTCGGAAATTTCCGCCGCTTTTTCAGAAAGTTTCTGCGACTGGTGAGAAGACTTCGCTTTTTGGAGCTTGGACCCAAGATAGTGCCTTGCAATTTCGAGATGAATCGCCTGTTTCGATTGAGCTTAAGTCAATTTCGAGTGAACTTGAGTTCGATAAGGCTATTGCAGATGCGGAGGAACGCAACGAGCTCGTGATTCTTGTTTGGATGGCTAACTGGTGCAGAAATTATATCTATTTAAAGCCTCAACTGGAAAGATTGGCTGCTGATTATTACCCCAGATTGCAATTCTACTGCATTGATGTCAATGTGGTGCCACACAAGCTAGTTGTTCGAGCCGGACTAGCTAAGATGCCAGCTATACAGCTATGGAAGGATGGTAAGAAACAAGATGAGGTGGTTGGTCTCTACAAAGCATATCTAGTTGTCAACGATGTTCGGAAAATGATTGAACGTGAGCTAACAGATGAGTGA

Protein sequence

MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATGEKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIERELTDE*
BLAST of Cucsa.308540 vs. Swiss-Prot
Match: TRL32_ARATH (Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana GN=WCRKC2 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-40
Identity = 86/176 (48.86%), Postives = 117/176 (66.48%), Query Frame = 1

Query: 27  SPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATGEKTSLFGAWTQDSALQFRDESPVSIE 86
           SP +   R   S F+R P + K+     K+    E+        QD  LQ  D+SPVS+E
Sbjct: 19  SPLVPPYRQTSSSFSR-PRNFKYHSFTDKICLAAERIRAVDIQKQDGGLQELDDSPVSVE 78

Query: 87  LKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCID 146
           L  I  E  FD+ + DA++  E V++VWMA WCR  IYLKP+LE+LAA++YPRL+FY +D
Sbjct: 79  LGPICGESHFDQVMEDAQKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVD 138

Query: 147 VNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIERE 203
           VN VP++LV RAG+     KMP IQLW+DG+KQ EV+G +KA+ VVN+VR+MIE +
Sbjct: 139 VNAVPYRLVSRAGVT----KMPTIQLWRDGQKQAEVIGGHKAHFVVNEVREMIEND 189

BLAST of Cucsa.308540 vs. Swiss-Prot
Match: TRL31_ARATH (Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana GN=WCRKC1 PE=2 SV=3)

HSP 1 Score: 115.2 bits (287), Expect = 8.8e-25
Identity = 52/120 (43.33%), Postives = 85/120 (70.83%), Query Frame = 1

Query: 82  PVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQ 141
           P ++E++ I++  E D  ++ A + ++ +I+ WMA+WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 142 FYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIER 201
           FY +DVN VP  LV R  ++    KMP IQLWK+ + ++EV+G +K +LV+ +VR++I +
Sbjct: 153 FYYVDVNKVPQTLVKRGNIS----KMPTIQLWKEDEMKEEVIGGHKGWLVIEEVRELINK 208

BLAST of Cucsa.308540 vs. Swiss-Prot
Match: TRL31_ORYSJ (Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica GN=Os02g0774100 PE=3 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 1.8e-22
Identity = 52/117 (44.44%), Postives = 82/117 (70.09%), Query Frame = 1

Query: 85  IELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYC 144
           +E++ I S  + D A+  + E  + +I+ WMA+WCR  IYLKP+LE++A + +P ++FY 
Sbjct: 80  VEMEEIDSMEKLDDALRWSVENKQPIIIDWMASWCRKCIYLKPRLEKIAGE-FPGVRFYF 139

Query: 145 IDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIER 202
           +DVN VP  +V R  +     KMP IQLWKDG+   EV+G +KA+LV+++VR+MI++
Sbjct: 140 VDVNKVPQTVVKRGNIT----KMPTIQLWKDGEWAAEVIGGHKAWLVMDEVREMIQK 191

BLAST of Cucsa.308540 vs. Swiss-Prot
Match: TRXH8_ARATH (Thioredoxin H8 OS=Arabidopsis thaliana GN=TRX8 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 7.0e-06
Identity = 35/118 (29.66%), Postives = 64/118 (54.24%), Query Frame = 1

Query: 69  WT-QDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKP 128
           WT +     F+  SP  +E+K+++   ++   +   ++ N+L+++ + A WC     L+P
Sbjct: 23  WTPRPEIYPFKVNSPCIVEIKNMN---QWKSRLNALKDTNKLLVIEFTAKWCGPCKTLEP 82

Query: 129 QLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGL 186
           +LE LAA  Y  ++F  IDV+V     ++   +   L  +PAI   K G++ D VVG+
Sbjct: 83  KLEELAAK-YTDVEFVKIDVDV-----LMSVWMEFNLSTLPAIVFMKRGREVDMVVGV 131

BLAST of Cucsa.308540 vs. TrEMBL
Match: A0A0A0KUX7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G622670 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 5.2e-109
Identity = 202/206 (98.06%), Postives = 202/206 (98.06%), Query Frame = 1

Query: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60
           MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG
Sbjct: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60

Query: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120
           EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR
Sbjct: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLA    KMPAIQLWKDGKKQD
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLA----KMPAIQLWKDGKKQD 180

Query: 181 EVVGLYKAYLVVNDVRKMIERELTDE 207
           EVVGLYKAYLVVNDVRKMIERELTDE
Sbjct: 181 EVVGLYKAYLVVNDVRKMIERELTDE 202

BLAST of Cucsa.308540 vs. TrEMBL
Match: D7TM63_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g04650 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-44
Identity = 97/182 (53.30%), Postives = 123/182 (67.58%), Query Frame = 1

Query: 24  LPPSPQLLSARLAGSGFNRLPLS---RKFPPLFQKVSATGEKTSLFGAWTQDSALQFRDE 83
           LP +P+  S  L       L +S   RK     QK+  +G K     AW Q+  LQ  D+
Sbjct: 15  LPTTPRGSSRILQQFSIPGLSISDFPRKSLNFPQKIGVSGGKVWALAAWNQEGPLQDLDD 74

Query: 84  SPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRL 143
           SPVS+ELK ISS+ +FD+ + +A++  E V +VWMA WCR  IYLKP+LE+LAA+YYPRL
Sbjct: 75  SPVSVELKPISSDTQFDRILGEAQQLEESVAIVWMATWCRKCIYLKPKLEKLAAEYYPRL 134

Query: 144 QFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIE 203
           +FYC+DVN VPHKLV RA +     KMP IQLWKD KKQ EV+G +KAY VVN+VR+MIE
Sbjct: 135 RFYCVDVNTVPHKLVARAAVT----KMPTIQLWKDSKKQAEVIGGHKAYFVVNEVREMIE 192

BLAST of Cucsa.308540 vs. TrEMBL
Match: A0A061FN59_THECC (WCRKC thioredoxin 2 OS=Theobroma cacao GN=TCM_043218 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-44
Identity = 89/167 (53.29%), Postives = 123/167 (73.65%), Query Frame = 1

Query: 38  SGFNRLPLSRKFPPLFQKVSATGEKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFD 97
           S F+ L L RK     ++++  G K  +F AW +D  L+  D++P+++EL+ I SE +FD
Sbjct: 40  SSFDGLSLKRKTLSFSERINRFGGKFGIFNAWKKDGYLEELDDAPLAVELQQICSESQFD 99

Query: 98  KAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVR 157
           + IA+A++  E +I++WMA+WCR  IYLKP+LE+LAA+YYPRL+FY +DVN VPH LV R
Sbjct: 100 RVIAEAQQLEESLIILWMASWCRKCIYLKPKLEKLAAEYYPRLRFYRVDVNTVPHNLVAR 159

Query: 158 AGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIERELT 205
           AG+     KMP IQLW+D KKQ EV+G +KAYLVVN+VR+MIE E T
Sbjct: 160 AGVT----KMPTIQLWRDSKKQAEVIGGHKAYLVVNEVREMIENECT 202

BLAST of Cucsa.308540 vs. TrEMBL
Match: A0A0D2UWR5_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G311900 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.5e-44
Identity = 86/160 (53.75%), Postives = 120/160 (75.00%), Query Frame = 1

Query: 45  LSRKFPPLFQKVSATGEKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAE 104
           L RK     +K++  G    +F AW ++  L+  D++P+++EL+ I SE +FD+ IA+A+
Sbjct: 47  LKRKILGFDEKINGFGGNFGIFSAWREEGQLEELDDAPLTVELQQICSESQFDRVIAEAQ 106

Query: 105 ERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGL 164
           +  E +I++WMA+WCR  IYLKP+LE+LAAD++PRL+FYC+DVN VPH LV RAG+    
Sbjct: 107 QLEESLIILWMASWCRKCIYLKPKLEKLAADFHPRLRFYCVDVNTVPHNLVARAGVT--- 166

Query: 165 EKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIERELT 205
            KMP IQLW+DGKKQ EV+G +KAYLVVN+VR+MIE E T
Sbjct: 167 -KMPTIQLWRDGKKQAEVIGGHKAYLVVNEVRQMIENECT 202

BLAST of Cucsa.308540 vs. TrEMBL
Match: M5X258_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009790mg PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.2e-42
Identity = 101/192 (52.60%), Postives = 128/192 (66.67%), Query Frame = 1

Query: 20  SSFDLPPSPQL----LSARLAG-----SGFNRLPLSRKFPPLFQKVSATGEKTSLFGAWT 79
           +S D P SP+L    LS RL G     S  N L  SRKF  + + + A         A +
Sbjct: 94  ASRDPPTSPELKISILSFRLRGPTRYPSSSNPLVFSRKFDEIRRPIRAVN-------ALS 153

Query: 80  QDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLE 139
           Q+ +LQ  D++PVS+ L  IS E +FD+ +A A++  E V++VWMA+WCR  IYLKP+LE
Sbjct: 154 QEGSLQELDDTPVSVALVPISGETQFDRVMAQAQQLEESVVVVWMASWCRKCIYLKPKLE 213

Query: 140 RLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYL 199
           +LAA+YYPRL FY +DVN VPHKLV RA +     KMP IQLWKDGKKQ EV+G +KAYL
Sbjct: 214 KLAAEYYPRLWFYSVDVNSVPHKLVARAEVT----KMPTIQLWKDGKKQAEVIGGHKAYL 273

Query: 200 VVNDVRKMIERE 203
           VVN+VR MIE E
Sbjct: 274 VVNEVRDMIENE 274

BLAST of Cucsa.308540 vs. TAIR10
Match: AT5G04260.1 (AT5G04260.1 WCRKC thioredoxin 2)

HSP 1 Score: 167.5 bits (423), Expect = 8.4e-42
Identity = 86/176 (48.86%), Postives = 117/176 (66.48%), Query Frame = 1

Query: 27  SPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATGEKTSLFGAWTQDSALQFRDESPVSIE 86
           SP +   R   S F+R P + K+     K+    E+        QD  LQ  D+SPVS+E
Sbjct: 19  SPLVPPYRQTSSSFSR-PRNFKYHSFTDKICLAAERIRAVDIQKQDGGLQELDDSPVSVE 78

Query: 87  LKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQFYCID 146
           L  I  E  FD+ + DA++  E V++VWMA WCR  IYLKP+LE+LAA++YPRL+FY +D
Sbjct: 79  LGPICGESHFDQVMEDAQKLGESVVIVWMAAWCRKCIYLKPKLEKLAAEFYPRLRFYHVD 138

Query: 147 VNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGLYKAYLVVNDVRKMIERE 203
           VN VP++LV RAG+     KMP IQLW+DG+KQ EV+G +KA+ VVN+VR+MIE +
Sbjct: 139 VNAVPYRLVSRAGVT----KMPTIQLWRDGQKQAEVIGGHKAHFVVNEVREMIEND 189

BLAST of Cucsa.308540 vs. TAIR10
Match: AT5G06690.2 (AT5G06690.2 WCRKC thioredoxin 1)

HSP 1 Score: 82.4 bits (202), Expect = 3.6e-16
Identity = 36/81 (44.44%), Postives = 57/81 (70.37%), Query Frame = 1

Query: 82  PVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKPQLERLAADYYPRLQ 141
           P ++E++ I++  E D  ++ A + ++ +I+ WMA+WCR  IYLKP+LE+LAA+Y  R +
Sbjct: 93  PTTLEMEPINNVEELDAVLSHARQLSQPIIIEWMASWCRKCIYLKPKLEKLAAEYNNRAK 152

Query: 142 FYCIDVNVVPHKLVVRAGLAV 163
           FY +DVN VP  LV R  ++V
Sbjct: 153 FYYVDVNKVPQTLVKRGNISV 173

BLAST of Cucsa.308540 vs. TAIR10
Match: AT1G69880.1 (AT1G69880.1 thioredoxin H-type 8)

HSP 1 Score: 52.4 bits (124), Expect = 3.9e-07
Identity = 35/118 (29.66%), Postives = 64/118 (54.24%), Query Frame = 1

Query: 69  WT-QDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCRNYIYLKP 128
           WT +     F+  SP  +E+K+++   ++   +   ++ N+L+++ + A WC     L+P
Sbjct: 23  WTPRPEIYPFKVNSPCIVEIKNMN---QWKSRLNALKDTNKLLVIEFTAKWCGPCKTLEP 82

Query: 129 QLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQDEVVGL 186
           +LE LAA  Y  ++F  IDV+V     ++   +   L  +PAI   K G++ D VVG+
Sbjct: 83  KLEELAAK-YTDVEFVKIDVDV-----LMSVWMEFNLSTLPAIVFMKRGREVDMVVGV 131

BLAST of Cucsa.308540 vs. NCBI nr
Match: gi|778706563|ref|XP_011655873.1| (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 408.3 bits (1048), Expect = 8.0e-111
Identity = 205/206 (99.51%), Postives = 205/206 (99.51%), Query Frame = 1

Query: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60
           MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG
Sbjct: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60

Query: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120
           EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR
Sbjct: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLE MPAIQLWKDGKKQD
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLE-MPAIQLWKDGKKQD 180

Query: 181 EVVGLYKAYLVVNDVRKMIERELTDE 207
           EVVGLYKAYLVVNDVRKMIERELTDE
Sbjct: 181 EVVGLYKAYLVVNDVRKMIERELTDE 205

BLAST of Cucsa.308540 vs. NCBI nr
Match: gi|778706568|ref|XP_004142496.2| (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 401.7 bits (1031), Expect = 7.5e-109
Identity = 202/206 (98.06%), Postives = 202/206 (98.06%), Query Frame = 1

Query: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60
           MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG
Sbjct: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60

Query: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120
           EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR
Sbjct: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLA    KMPAIQLWKDGKKQD
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLA----KMPAIQLWKDGKKQD 180

Query: 181 EVVGLYKAYLVVNDVRKMIERELTDE 207
           EVVGLYKAYLVVNDVRKMIERELTDE
Sbjct: 181 EVVGLYKAYLVVNDVRKMIERELTDE 202

BLAST of Cucsa.308540 vs. NCBI nr
Match: gi|659092008|ref|XP_008446851.1| (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X7 [Cucumis melo])

HSP 1 Score: 401.7 bits (1031), Expect = 7.5e-109
Identity = 200/206 (97.09%), Postives = 202/206 (98.06%), Query Frame = 1

Query: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60
           MVKALPLH GIVKSFPKF SSFDLPPSPQLLSARL GSGFNRLPLSRKFPP+FQKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120
           EKTSL GAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD 180
           NYIYLKPQLERLAADYYPRLQFYCIDVN+VPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNMVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD 180

Query: 181 EVVGLYKAYLVVNDVRKMIERELTDE 207
           EVVGLYKAYLVVNDVRKMIERELTDE
Sbjct: 181 EVVGLYKAYLVVNDVRKMIERELTDE 206

BLAST of Cucsa.308540 vs. NCBI nr
Match: gi|778706571|ref|XP_011655874.1| (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 399.4 bits (1025), Expect = 3.7e-108
Identity = 201/206 (97.57%), Postives = 201/206 (97.57%), Query Frame = 1

Query: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60
           MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG
Sbjct: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60

Query: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120
           EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR
Sbjct: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLWKDGKKQD 180
           NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLA     MPAIQLWKDGKKQD
Sbjct: 121 NYIYLKPQLERLAADYYPRLQFYCIDVNVVPHKLVVRAGLA-----MPAIQLWKDGKKQD 180

Query: 181 EVVGLYKAYLVVNDVRKMIERELTDE 207
           EVVGLYKAYLVVNDVRKMIERELTDE
Sbjct: 181 EVVGLYKAYLVVNDVRKMIERELTDE 201

BLAST of Cucsa.308540 vs. NCBI nr
Match: gi|659092000|ref|XP_008446847.1| (PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 394.8 bits (1013), Expect = 9.2e-107
Identity = 200/213 (93.90%), Postives = 202/213 (94.84%), Query Frame = 1

Query: 1   MVKALPLHHGIVKSFPKFGSSFDLPPSPQLLSARLAGSGFNRLPLSRKFPPLFQKVSATG 60
           MVKALPLH GIVKSFPKF SSFDLPPSPQLLSARL GSGFNRLPLSRKFPP+FQKVSATG
Sbjct: 1   MVKALPLHLGIVKSFPKFDSSFDLPPSPQLLSARLPGSGFNRLPLSRKFPPIFQKVSATG 60

Query: 61  EKTSLFGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120
           EKTSL GAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR
Sbjct: 61  EKTSLLGAWTQDSALQFRDESPVSIELKSISSELEFDKAIADAEERNELVILVWMANWCR 120

Query: 121 NYIYLKPQLERLAADYYP-------RLQFYCIDVNVVPHKLVVRAGLAVGLEKMPAIQLW 180
           NYIYLKPQLERLAADYYP       RLQFYCIDVN+VPHKLVVRAGLAVGLEKMPAIQLW
Sbjct: 121 NYIYLKPQLERLAADYYPSMCLMNCRLQFYCIDVNMVPHKLVVRAGLAVGLEKMPAIQLW 180

Query: 181 KDGKKQDEVVGLYKAYLVVNDVRKMIERELTDE 207
           KDGKKQDEVVGLYKAYLVVNDVRKMIERELTDE
Sbjct: 181 KDGKKQDEVVGLYKAYLVVNDVRKMIERELTDE 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TRL32_ARATH1.5e-4048.86Thioredoxin-like 3-2, chloroplastic OS=Arabidopsis thaliana GN=WCRKC2 PE=2 SV=1[more]
TRL31_ARATH8.8e-2543.33Thioredoxin-like 3-1, chloroplastic OS=Arabidopsis thaliana GN=WCRKC1 PE=2 SV=3[more]
TRL31_ORYSJ1.8e-2244.44Thioredoxin-like 3-1, chloroplastic OS=Oryza sativa subsp. japonica GN=Os02g0774... [more]
TRXH8_ARATH7.0e-0629.66Thioredoxin H8 OS=Arabidopsis thaliana GN=TRX8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KUX7_CUCSA5.2e-10998.06Uncharacterized protein OS=Cucumis sativus GN=Csa_5G622670 PE=4 SV=1[more]
D7TM63_VITVI1.2e-4453.30Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g04650 PE=4 SV=... [more]
A0A061FN59_THECC2.0e-4453.29WCRKC thioredoxin 2 OS=Theobroma cacao GN=TCM_043218 PE=4 SV=1[more]
A0A0D2UWR5_GOSRA3.5e-4453.75Uncharacterized protein OS=Gossypium raimondii GN=B456_009G311900 PE=4 SV=1[more]
M5X258_PRUPE3.2e-4252.60Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009790mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G04260.18.4e-4248.86 WCRKC thioredoxin 2[more]
AT5G06690.23.6e-1644.44 WCRKC thioredoxin 1[more]
AT1G69880.13.9e-0729.66 thioredoxin H-type 8[more]
Match NameE-valueIdentityDescription
gi|778706563|ref|XP_011655873.1|8.0e-11199.51PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X1 [Cucumis sativus][more]
gi|778706568|ref|XP_004142496.2|7.5e-10998.06PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X2 [Cucumis sativus][more]
gi|659092008|ref|XP_008446851.1|7.5e-10997.09PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X7 [Cucumis melo][more]
gi|778706571|ref|XP_011655874.1|3.7e-10897.57PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X3 [Cucumis sativus][more]
gi|659092000|ref|XP_008446847.1|9.2e-10793.90PREDICTED: thioredoxin-like 3-2, chloroplastic isoform X3 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005746Thioredoxin
IPR012336Thioredoxin-like_fold
IPR013766Thioredoxin_domain
IPR005746Thioredoxin
IPR012336Thioredoxin-like_fold
IPR013766Thioredoxin_domain
Vocabulary: Biological Process
TermDefinition
GO:0006662glycerol ether metabolic process
GO:0045454cell redox homeostasis
GO:0006662glycerol ether metabolic process
GO:0045454cell redox homeostasis
Vocabulary: Molecular Function
TermDefinition
GO:0015035protein disulfide oxidoreductase activity
GO:0015035protein disulfide oxidoreductase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045454 cell redox homeostasis
biological_process GO:0006662 glycerol ether metabolic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005623 cell
cellular_component GO:0005575 cellular_component
molecular_function GO:0015035 protein disulfide oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.308540.2Cucsa.308540.2mRNA
Cucsa.308540.1Cucsa.308540.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005746ThioredoxinPANTHERPTHR10438THIOREDOXINcoord: 24..198
score: 3.1
IPR012336Thioredoxin-like foldGENE3DG3DSA:3.40.30.10coord: 87..195
score: 5.1
IPR012336Thioredoxin-like foldunknownSSF52833Thioredoxin-likecoord: 87..196
score: 1.98
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 93..185
score: 3.
IPR013766Thioredoxin domainPROFILEPS51352THIOREDOXIN_2coord: 75..200
score: 8
NoneNo IPR availablePANTHERPTHR10438:SF297THIOREDOXIN-LIKE 3-2, CHLOROPLASTICcoord: 24..198
score: 3.1

The following gene(s) are paralogous to this gene:

None