Cucsa.218490 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.218490
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Phloem filament protein
Location	scaffold01658 : 587338 .. 587941 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCACAAAATTTTCATTACTATCAAATATCAACTAAAGCTCTCACAAAAAAATGACAATTGGTATTGGTGGAGGAATTGGCCTTGGTCTTCCTGGTCTCAATATAAAGATCGGCGGAGGCATCGGTCTTGGGAATAAAAGgCCGGGGGGgTGCAAGAGTCATGATCCATCAAAAAAATGGAGTGTATGTCCCAATGATCGCGTTGTAGAAATATATGCTAGGTTTGCAGTGGATGAGTACAACAGAAAACATGGACGTAATCTTGTGTTTCAATCAGTTTTGGAAGCTTGGGTTTATGTTTATCCTTGTGGCAAGAAAGAGTATAGTATTGAATTAGTAGTAAGAGAAGGGTGTGGGAATCATGTCCTCAAGTATCATGCTGTTGTGACTGAAACAGGCTGTGCTGCTCGCAGGAAGACTCTGGTGTCTTTCGACCAAATCGACGACTAAAAGAGAAGTTATATGCATGCAATCAAAGTTTATACTTATGTGTGAGAAGTACACAATAATAAATCTTATATGATATTGTACTGTTCTACTCTCTTTCACTATCTCTGTTCTAAAATGATAATGGAGGCCAAACTACGTTTTTAGTCCCTAACCC

mRNA sequence

ACCACAAAATTTTCATTACTATCAAATATCAACTAAAGCTCTCACAAAAAAATGACAATTGGTATTGGTGGAGGAATTGGCCTTGGTCTTCCTGGTCTCAATATAAAGATCGGCGGAGGCATCGGTCTTGGGAATAAAAGGCCGGGGGGGTGCAAGAGTCATGATCCATCAAAAAAATGGAGTGTATGTCCCAATGATCGCGTTGTAGAAATATATGCTAGGTTTGCAGTGGATGAGTACAACAGAAAACATGGACGTAATCTTGTGTTTCAATCAGTTTTGGAAGCTTGGGTTTATGTTTATCCTTGTGGCAAGAAAGAGTATAGTATTGAATTAGTAGTAAGAGAAGGGTGTGGGAATCATGTCCTCAAGTATCATGCTGTTGTGACTGAAACAGGCTGTGCTGCTCGCAGGAAGACTCTGGTGTCTTTCGACCAAATCGACGACTAAAAGAGAAGTTATATGCATGCAATCAAAGTTTATACTTATGTGTGAGAAGTACACAATAATAAATCTTATATGATATTGTACTGTTCTACTCTCTTTCACTATCTCTGTTCTAAAATGATAATGGAGGCCAAACTACGTTTTTAGTCCCTAACCC

Coding sequence (CDS)

ATGACAATTGGTATTGGTGGAGGAATTGGCCTTGGTCTTCCTGGTCTCAATATAAAGATCGGCGGAGGCATCGGTCTTGGGAATAAAAGgCCGGGGGGgTGCAAGAGTCATGATCCATCAAAAAAATGGAGTGTATGTCCCAATGATCGCGTTGTAGAAATATATGCTAGGTTTGCAGTGGATGAGTACAACAGAAAACATGGACGTAATCTTGTGTTTCAATCAGTTTTGGAAGCTTGGGTTTATGTTTATCCTTGTGGCAAGAAAGAGTATAGTATTGAATTAGTAGTAAGAGAAGGGTGTGGGAATCATGTCCTCAAGTATCATGCTGTTGTGACTGAAACAGGCTGTGCTGCTCGCAGGAAGACTCTGGTGTCTTTCGACCAAATCGACGACTAA

Protein sequence

MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAARRKTLVSFDQIDD*

Homology

[Close]

BLAST of Cucsa.218490 vs. TrEMBL
Match: A0A0A0K281_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067350 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 1.1e-72
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAV 60
           MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAV
Sbjct: 1   MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAV 60

Query: 61  DEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAAR 120
           DEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAAR
Sbjct: 61  DEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAAR 120

Query: 121 RKTLVSFDQIDD 133
           RKTLVSFDQIDD
Sbjct: 121 RKTLVSFDQIDD 132

[Close]

BLAST of Cucsa.218490 vs. TrEMBL
Match: A0A0A0K660_CUCSA (Phloem filament protein OS=Cucumis sativus GN=Csa_7G067370 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 9.6e-48
Identity = 93/129 (72.09%), Postives = 109/129 (84.50%), Query Frame = 1


Query: 4   GIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAVDEY 63
           G+ GG+G G    ++KIGGGI LG+KRPGGCKSHDPSKKW +CPND+VVE  A+FAV E+
Sbjct: 7   GLDGGLGFGFSPFSLKIGGGIDLGSKRPGGCKSHDPSKKWVICPNDQVVEA-AKFAVAEF 66

Query: 64  NRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAARRKT 123
           N+K+G NLVFQ VLEAWV+  PCGKKEYSIELVV +GC N VLK+HAVVTET CAAR++T
Sbjct: 67  NKKYGNNLVFQVVLEAWVFTNPCGKKEYSIELVVIDGCLNRVLKFHAVVTETPCAARKRT 126

Query: 124 LVSFDQIDD 133
           LVSFDQI D
Sbjct: 127 LVSFDQIVD 134

[Close]

BLAST of Cucsa.218490 vs. TrEMBL
Match: A0A0A0K477_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067390 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.7e-32
Identity = 78/137 (56.93%), Postives = 96/137 (70.07%), Query Frame = 1


Query: 1   MTIGIGGGIGLGL--PGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPN---DRVVEIY 60
           MTIGIGGGIG+G+  PG+ + IGGGIG+G+++ GGCKSHDPSKKWS  PN   D+V+   
Sbjct: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRK-GGCKSHDPSKKWSQVPNAQADQVIINV 83

Query: 61  ARFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTET 120
           A FAV  +N+K+G  LVF +VLEAWV V P GKKEYSIELV ++ C N  L +HA+V E 
Sbjct: 84  ANFAVAAFNQKYGNKLVFHAVLEAWVVVLPDGKKEYSIELVAKD-CLNRCLYFHAIVIEI 143

Query: 121 GCAARRKTLVSFDQIDD 133
                   L SF QI D
Sbjct: 144 VGVPPGWNLFSFTQISD 158

[Close]

BLAST of Cucsa.218490 vs. TrEMBL
Match: E5GCR5_CUCME (Phloem filament protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.8e-31
Identity = 78/134 (58.21%), Postives = 96/134 (71.64%), Query Frame = 1


Query: 1   MTIGIGGGI--GLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCP--NDRVVEIYA 60
           MT+GIGGGI  GLG+PG+NI IGGGIG+  ++ GGCKSHDPSKKW+     ND  +   A
Sbjct: 1   MTLGIGGGISIGLGIPGINISIGGGIGIRGRK-GGCKSHDPSKKWTEIKSVNDPAIIEIA 60

Query: 61  RFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETG 120
           +FAV  +N+KHG NL FQ+VLEAWV V+  GKKEYSIELV ++ C +  L+YHAVV E  
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKD-CLHRCLQYHAVVIEIC 120

Query: 121 CAARRKTLVSFDQI 131
                  LVSF+QI
Sbjct: 121 GTPPGWNLVSFNQI 132

[Close]

BLAST of Cucsa.218490 vs. TrEMBL
Match: E5GCR6_CUCME (Phloem filament protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.8e-25
Identity = 72/137 (52.55%), Postives = 94/137 (68.61%), Query Frame = 1


Query: 1   MTIGIGG--GIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIY--A 60
           MT+GIGG  GIG GLPG NI IGGGIG+G  R GGCKSH+PSKKW    N +  ++   A
Sbjct: 1   MTLGIGGRIGIGFGLPGFNISIGGGIGIGG-RKGGCKSHNPSKKWIQLHNVKAPDVLEAA 60

Query: 61  RFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETG 120
           +FAVD +N+K+  NL FQ V EAWV+     +KEYSIELV ++ C + +L++HAVV E  
Sbjct: 61  KFAVDAFNKKYENNLEFQYVTEAWVHEVN-NEKEYSIELVAKD-CFHRLLEFHAVVIEKT 120

Query: 121 CAARR-KTLVSFDQIDD 133
              ++   LVSF++I D
Sbjct: 121 VDHKKIWILVSFERIFD 134

[Close]

BLAST of Cucsa.218490 vs. NCBI nr
Match: gi|778724610|ref|XP_011658838.1| (PREDICTED: uncharacterized protein LOC105436096 [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 1.6e-72
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 1


Query: 1   MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAV 60
           MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAV
Sbjct: 1   MTIGIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAV 60

Query: 61  DEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAAR 120
           DEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAAR
Sbjct: 61  DEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAAR 120

Query: 121 RKTLVSFDQIDD 133
           RKTLVSFDQIDD
Sbjct: 121 RKTLVSFDQIDD 132

[Close]

BLAST of Cucsa.218490 vs. NCBI nr
Match: gi|778724613|ref|XP_011658839.1| (PREDICTED: uncharacterized protein LOC105436097 [Cucumis sativus])

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-47
Identity = 93/129 (72.09%), Postives = 109/129 (84.50%), Query Frame = 1


Query: 4   GIGGGIGLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPNDRVVEIYARFAVDEY 63
           G+ GG+G G    ++KIGGGI LG+KRPGGCKSHDPSKKW +CPND+VVE  A+FAV E+
Sbjct: 7   GLDGGLGFGFSPFSLKIGGGIDLGSKRPGGCKSHDPSKKWVICPNDQVVEA-AKFAVAEF 66

Query: 64  NRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETGCAARRKT 123
           N+K+G NLVFQ VLEAWV+  PCGKKEYSIELVV +GC N VLK+HAVVTET CAAR++T
Sbjct: 67  NKKYGNNLVFQVVLEAWVFTNPCGKKEYSIELVVIDGCLNRVLKFHAVVTETPCAARKRT 126

Query: 124 LVSFDQIDD 133
           LVSFDQI D
Sbjct: 127 LVSFDQIVD 134

[Close]

BLAST of Cucsa.218490 vs. NCBI nr
Match: gi|778724618|ref|XP_011658840.1| (PREDICTED: uncharacterized protein LOC105436098 [Cucumis sativus])

HSP 1 Score: 145.2 bits (365), Expect = 8.1e-32
Identity = 78/137 (56.93%), Postives = 96/137 (70.07%), Query Frame = 1


Query: 1   MTIGIGGGIGLGL--PGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCPN---DRVVEIY 60
           MTIGIGGGIG+G+  PG+ + IGGGIG+G+++ GGCKSHDPSKKWS  PN   D+V+   
Sbjct: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRK-GGCKSHDPSKKWSQVPNAQADQVIINV 83

Query: 61  ARFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTET 120
           A FAV  +N+K+G  LVF +VLEAWV V P GKKEYSIELV ++ C N  L +HA+V E 
Sbjct: 84  ANFAVAAFNQKYGNKLVFHAVLEAWVVVLPDGKKEYSIELVAKD-CLNRCLYFHAIVIEI 143

Query: 121 GCAARRKTLVSFDQIDD 133
                   L SF QI D
Sbjct: 144 VGVPPGWNLFSFTQISD 158

[Close]

BLAST of Cucsa.218490 vs. NCBI nr
Match: gi|659110227|ref|XP_008455116.1| (PREDICTED: uncharacterized protein LOC103495368 [Cucumis melo])

HSP 1 Score: 142.1 bits (357), Expect = 6.9e-31
Identity = 78/134 (58.21%), Postives = 96/134 (71.64%), Query Frame = 1


Query: 1   MTIGIGGGI--GLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCP--NDRVVEIYA 60
           MT+GIGGGI  GLG+PG+NI IGGGIG+  ++ GGCKSHDPSKKW+     ND  +   A
Sbjct: 26  MTLGIGGGISIGLGIPGINISIGGGIGIRGRK-GGCKSHDPSKKWTEIKSVNDPAIIEIA 85

Query: 61  RFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETG 120
           +FAV  +N+KHG NL FQ+VLEAWV V+  GKKEYSIELV ++ C +  L+YHAVV E  
Sbjct: 86  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKD-CLHRCLQYHAVVIEIC 145

Query: 121 CAARRKTLVSFDQI 131
                  LVSF+QI
Sbjct: 146 GTPPGWNLVSFNQI 157

[Close]

BLAST of Cucsa.218490 vs. NCBI nr
Match: gi|307136458|gb|ADN34263.1| (phloem filament protein [Cucumis melo subsp. melo])

HSP 1 Score: 142.1 bits (357), Expect = 6.9e-31
Identity = 78/134 (58.21%), Postives = 96/134 (71.64%), Query Frame = 1


Query: 1   MTIGIGGGI--GLGLPGLNIKIGGGIGLGNKRPGGCKSHDPSKKWSVCP--NDRVVEIYA 60
           MT+GIGGGI  GLG+PG+NI IGGGIG+  ++ GGCKSHDPSKKW+     ND  +   A
Sbjct: 1   MTLGIGGGISIGLGIPGINISIGGGIGIRGRK-GGCKSHDPSKKWTEIKSVNDPAIIEIA 60

Query: 61  RFAVDEYNRKHGRNLVFQSVLEAWVYVYPCGKKEYSIELVVREGCGNHVLKYHAVVTETG 120
           +FAV  +N+KHG NL FQ+VLEAWV V+  GKKEYSIELV ++ C +  L+YHAVV E  
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKD-CLHRCLQYHAVVIEIC 120

Query: 121 CAARRKTLVSFDQI 131
                  LVSF+QI
Sbjct: 121 GTPPGWNLVSFNQI 132

The following BLAST results are available for this feature:

BLAST of Cucsa.218490 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K281_CUCSA	1.1e-72	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067350 PE=4 SV=1	[more]
A0A0A0K660_CUCSA	9.6e-48	72.09	Phloem filament protein OS=Cucumis sativus GN=Csa_7G067370 PE=4 SV=1	[more]
A0A0A0K477_CUCSA	5.7e-32	56.93	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G067390 PE=4 SV=1	[more]
E5GCR5_CUCME	4.8e-31	58.21	Phloem filament protein OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
E5GCR6_CUCME	1.8e-25	52.55	Phloem filament protein OS=Cucumis melo subsp. melo PE=4 SV=1	[more]

BLAST of Cucsa.218490 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778724610\|ref\|XP_011658838.1\|	1.6e-72	100.00	PREDICTED: uncharacterized protein LOC105436096 [Cucumis sativus]	[more]
gi\|778724613\|ref\|XP_011658839.1\|	1.4e-47	72.09	PREDICTED: uncharacterized protein LOC105436097 [Cucumis sativus]	[more]
gi\|778724618\|ref\|XP_011658840.1\|	8.1e-32	56.93	PREDICTED: uncharacterized protein LOC105436098 [Cucumis sativus]	[more]
gi\|659110227\|ref\|XP_008455116.1\|	6.9e-31	58.21	PREDICTED: uncharacterized protein LOC103495368 [Cucumis melo]	[more]
gi\|307136458\|gb\|ADN34263.1\|	6.9e-31	58.21	phloem filament protein [Cucumis melo subsp. melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR020381	Proteinase inhibitor I25, cystatin, conserved region

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.218490.1	Cucsa.218490.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020381	Proteinase inhibitor I25, cystatin, conserved region	PFAM	PF16845	SQAPI	coord: 49..129 score: 2.2
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 47..117 score: 5.
None	No IPR available	unknown	SSF54403	Cystatin/monellin	coord: 43..130 score: 3.19

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.218490	Csa7G067350	Cucumber (Chinese Long) v2	cgycuB322
Cucsa.218490	MELO3C018579	Melon (DHL92) v3.5.1	cgymeB379
Cucsa.218490	CSPI07G06160	Wild cucumber (PI 183967)	cgycpiB336
Cucsa.218490	MELO3C018579.2	Melon (DHL92) v3.6.1	cgymedB377
Cucsa.218490	CsaV3_7G007130	Cucumber (Chinese Long) v3	cgycucB342

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.218490	Cucurbita pepo (Zucchini)	cgycpeB0594
Cucsa.218490	Cucurbita pepo (Zucchini)	cgycpeB0599
Cucsa.218490	Cucurbita pepo (Zucchini)	cgycpeB0602
Cucsa.218490	Bottle gourd (USVL1VR-Ls)	cgylsiB366
Cucsa.218490	Bottle gourd (USVL1VR-Ls)	cgylsiB367
Cucsa.218490	Melon (DHL92) v3.6.1	cgymedB381
Cucsa.218490	Silver-seed gourd	carcgyB0339
Cucsa.218490	Silver-seed gourd	carcgyB0435
Cucsa.218490	Silver-seed gourd	carcgyB0825
Cucsa.218490	Watermelon (97103) v2	cgywmbB393
Cucsa.218490	Watermelon (97103) v2	cgywmbB399
Cucsa.218490	Wax gourd	cgywgoB443
Cucsa.218490	Wax gourd	cgywgoB445
Cucsa.218490	Cucurbita maxima (Rimu)	cgycmaB0620
Cucsa.218490	Cucurbita maxima (Rimu)	cgycmaB0624
Cucsa.218490	Cucurbita maxima (Rimu)	cgycmaB0629
Cucsa.218490	Cucurbita moschata (Rifu)	cgycmoB0624
Cucsa.218490	Cucurbita moschata (Rifu)	cgycmoB0627
Cucsa.218490	Cucurbita moschata (Rifu)	cgycmoB0632
Cucsa.218490	Wild cucumber (PI 183967)	cgycpiB335
Cucsa.218490	Melon (DHL92) v3.5.1	cgymeB383
Cucsa.218490	Watermelon (Charleston Gray)	cgywcgB391
Cucsa.218490	Watermelon (Charleston Gray)	cgywcgB393
Cucsa.218490	Watermelon (97103) v1	cgywmB415
Cucsa.218490	Watermelon (97103) v1	cgywmB421