Cucsa.189580 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.189580
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	sphingoid base hydroxylase 2
Location	scaffold01305 : 432159 .. 434876 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAAAAAAAAAAGGGAAAAAGAAAAAAAAAGAAAAAGAAAAAATCTCTCTTCGTGTAAACCAGAGTGGGAAGGTTGTTCCCGATTTCCTTCCCAACGCCTAGCAGATTTCTGATTTGGGGATTTATGGTTCTCTCTTCCAATCTCTAAAATCTCTACCTCTTTCCCTTTTCAATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGGTGACCTTCTTTCTTTTTTTTNTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTCTTTTTTTTTTTTTTTTTTCTTTTACCTATTTTTTCAATTCTTAATCGTTGCTCTTTGTTAATTTTGTTGTTATTAAGCTCATTTCTGATTCTAATTGTTTGTGAAACTTACCCTAAAATATTTCAACTGAGATTGTATTTTGCTCTAGACTTTGCCGAAACTCTTTGTTTGATCTTCCTGAATTTTTCTTGGTTTATCACCTCTTTGTGGTACAGCTTGATTATAGTTTTACTAGACTGCATACAAATTCGTTGTGGGTTTTCTTGTTTCTGGACCTATTCAGTTTGACAAAAATCTAAATAATTACTTCTCTTCTGTTTACATGTGTTTTAAATTTTGATTTTGACAAGAACAGACTAGACACTGGATGGGGGGAGTGAAGAAGCAATGTCATTGTTGACCTATTATCAACTTTCGGTTTCTTATAATTACAACTATTGTATATCTTAAACAGGATTTGTTGCTTACTTTTGTTACTTTAGTTCGAATTGATGGAGCAAAATTTTAAACCTTTTCACATCAAAATAATTTAGATGGCAATTGAATTTTTTTTAATTTGGTTCAAGAATAAGAGGAGTTAATGATTCTAAGTTAAGGAAGTTGAAGGACAAAATCTGGTTATTAGCTTTTGACTGTAAGTATGTTTTGTTTCGAACACTACAAGGAAGGCTTTTGGTGAATACTTTTAACTTCAAGATAGACGTGAACAAAATTTTTGCATATTACCCCTTTTTCATAGCTAAATATTCTCTATGAGATGATTTTTTGGACTGGAAAGTTATGGTCTAAAACATGATATTTTTGTTTTCTTTGTAAATTAAATTCCATTTCCTTTAAGTATATTGACCTTTTGATTTGTCTATTCGTTTTAGGTTACATGTTTATTCACAAGTTTTAGGACCAATCCCCATTTGTGTTCTGCATCGGCACTCGGTTGGAAGTCACCAACTTGCAAACAAAAAAATCTTGAACTTCTGTTTAATTTTGTGGTGTTTTGCTTGTATTTCCTATAATGTTGTTGCCATTGCTTCTAATCCGTGAAGTTCCTTTTCTTTTTGAGTCCCATGCTAAACTAGTTTAGCAGTTGTTCGTGTGAAATATTGTAGACTGAACTAAAACTATTGTTATTATTAACCTCATCCAAGTGCTGTTAGATGAATTGTTCAGTTTGTCGTCCCTGTGGTTAAAGTGGCAAGTCCTGAACTCAAATGTTTTGAGCATACGAGCTCAAACTAAGATGCCACCTATTTGGGGCATCTTAGAAATCCTGGTTTTGTAATATTCTAAAGTTTAGTAGTTGTCTCGTGAGATTATTCCAAATGTATGTAAACTAACTCAGAGACTTAAGTTTTTGAAATAGAGAAAAGAAAAGGAATCAATGAGAACTTTGGCATAAGTCATTGATATTATGTGGGGTGTCTCTGCACTTACGTTCGAGTTTGGTCTCTGAATTTATAATTGCATTTTGTTATGTTCAGTTGACTGGAAATGATGATGGAAGCAATGCAGTTCCAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCATTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGATTGGTGGGGCGTTGTCATTTTTAGCATCTGGTATGACTCCTAGAATCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAAGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAAATACCGTAAGCAGCAATCAGTTGCTTATATTATATTATTTGTATCCCACAACTATATGATTTGTATATTACTCCTATTGTTGCCATTTTTATACTTTTTCCCCCATTTTCCCTCTCTTCCCTAACATAGATTTGAAACATGAGTTCAGATGAGCTAGGTTGTGTTTTGATATATATATATTTTT

mRNA sequence

aaataaaaaaaaaagggaaaaagaaaaaaaaagaaaaagaaaaaaTCTCTCTTCGTGTAAACCAGAGTGGGAAGGTTGTTCCCGATTTCCTTCCCAACGCCTAGCAGATTTCTGATTTGGGGATTTATGGTTCTCTCTTCCAATCTCTAAAATCTCTACCTCTTTCCCTTTTCAATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGTTGACTGGAAATGATGATGGAAGCAATGCAGTTCCAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCATTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGATTGGTGGGGCGTTGTCATTTTTAGCATCTGGTATGACTCCTAGAATCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAAGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAAATACCGTAAGCAGCAATCAGTTGCTTATATTATATTATTTGTATCCCACAACTATATGATTTGTATATTACTCCTATTGTTGCCATTTTTATACTTTTTCCCCCATTTTCCCTCTCTTCCCTAACATAGATTTGAAACATGAGTTCAGATGAGCTAGGTTGTGTTTTGatatatatatattttt

Coding sequence (CDS)

ATGGAATTCACCATCTCCGATGATTTGTTGGGTACTTTCGTCCCTATTTTCGTTTATTGGTTCTATTCTGGCTTATACCTTCTTCTTGGTTTCTTTGACAATTATCGATTGCACTCCAAGACAGATGAGGATGAGAAGAACTTGGTTTCTAAATCAACTGTTGTTCGGGGCGTTCTTTTTCAGCAATTCATTCAGGCTATTGTCGCCATCATCCTCTTTAAGTTGACTGGAAATGATGATGGAAGCAATGCAGTTCCAAAGTCATGGCCAATGGTTGTTTTGCAATTTTTAACTGCGATGTTCATTCTAGACACGTGGCAGTACTTTGCACACCGATACATGCATCATAACAAGTTTCTGTACAGACACATTCATTCTCAGCACCATAGGCTTGTTGTTCCATATGCATTTGGGGCTTTGTATAATCATCCAGTGGAAGGTCTACTGCTTGACACGATTGGTGGGGCGTTGTCATTTTTAGCATCTGGTATGACTCCTAGAATCTCCATCTTCTTCTTTTCCTTTGCCACTATTAAGACGGTAGACGATCACTGTGGATTATGGCTGCCTGGTAATCTCTTCCACATCTTATTTCGCAACAATACTGCATATCACGACGTCCACCATCAACTATACGGCAGCAAGTTTAATTTTTCACAGCCTTTCTTTGTTACATGGGATAGAATACTTGGTACCTATATGCCTTACTCCTTGGAGAAGAGAGCAAGCGGCGGCTTTGAAGCACGACCAAAGATTGAATAA

Protein sequence

MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEKRASGGFEARPKIE*

Homology

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BLAST of Cucsa.189580 vs. Swiss-Prot
Match: SBH1_ARATH (Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana GN=SBH1 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 3.6e-113
Identity = 188/254 (74.02%), Postives = 220/254 (86.61%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYW YSG+Y+ L   ++YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF +TG+D +   A   S+ ++  QF+TAM +LDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPYA+GALYNHPVEGLLLDTIGGALSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRASGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

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BLAST of Cucsa.189580 vs. Swiss-Prot
Match: SBH2_ARATH (Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana GN=SBH2 PE=1 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 6.2e-113
Identity = 188/251 (74.90%), Postives = 216/251 (86.06%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  D YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG+D D +     S  ++  QF+ AM ++DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPY++GALYNHP+EGLLLDTIGGALSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FHI F NN+AYHDVHHQLYG+K+NFSQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRASGGFEARP 251
           KRA+GGFE RP
Sbjct: 242 KRANGGFETRP 252

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BLAST of Cucsa.189580 vs. Swiss-Prot
Match: SUR2_SCHPO (Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=sur2 PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-56
Identity = 109/253 (43.08%), Postives = 149/253 (58.89%), Query Frame = 1


Query: 15  PIFVYWFYSGL-----YLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQAIVA 74
           P+ +YW  S       Y+ L  F+ YR+H   +   +N V +  VV+ VLFQQ  + +V 
Sbjct: 21  PVIIYWVASAFFGFLHYIELPVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQLCEVVVG 80

Query: 75  IILFKLTGN----DDGSNAV------PKSWPMVV-----------------LQFLTAMFI 134
           I L    G     D+    +       K+ P ++                  Q+  A FI
Sbjct: 81  IALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQYFFAFFI 140

Query: 135 LDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLAS 194
           +D+WQYF HRY+H+NK LY  IH+ HHRL VPYA GALYNHP EGL+LDT G  +++LA+
Sbjct: 141 IDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAGVAYLAA 200

Query: 195 GMTPRISIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPF 236
           G++P+ ++ FF+ +T+KTVDDHCG   P +   + F NN  YHD+HHQ YG + NFSQPF
Sbjct: 201 GLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQKNFSQPF 260

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BLAST of Cucsa.189580 vs. Swiss-Prot
Match: SUR2_YEAST (Sphingolipid C4-hydroxylase SUR2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SUR2 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.3e-50
Identity = 102/252 (40.48%), Postives = 142/252 (56.35%), Query Frame = 1


Query: 5   ISDDLLGTFVPIFVYWFYSGLYLLLGFF---DNYRLHSKTDEDEKNLVSKSTVVRGVLFQ 64
           +SD +L    P+  YW  SG++ ++  F   + YR+H   +  ++N  S+  V   V+ Q
Sbjct: 46  MSDGVLALVAPVVAYWALSGIFHVIDTFHLAEKYRIHPSEEVAKRNKASRMHVFLEVILQ 105

Query: 65  QFIQAIVAIILFK-----LTGNDDGS---------NAVPKS-------WPMVVLQFLTAM 124
             IQ IV +I        +TG ++ +           +P +       + M  L+     
Sbjct: 106 HIIQTIVGLIFMHFEPIYMTGFEENAMWKLRADLPRIIPDAAIYYGYMYGMSALKIFAGF 165

Query: 125 FILDTWQYFAHRYMHHNKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFL 184
             +DTWQYF HR MH NK LY+  HS HH L VPYA+GAL+N+PVEG LLDT+G  ++  
Sbjct: 166 LFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPYAYGALFNNPVEGFLLDTLGTGIAMT 225

Query: 185 ASGMTPRISIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQ 233
            + +T R  I  F+FAT+KTVDDHCG  LP + F  LF NN  YHD+HHQ +G K NF+Q
Sbjct: 226 LTHLTHREQIILFTFATMKTVDDHCGYALPLDPFQWLFPNNAVYHDIHHQQFGIKTNFAQ 285

[Close]

BLAST of Cucsa.189580 vs. Swiss-Prot
Match: MSMOA_DICDI (Putative methylsterol monooxygenase DDB_G0270946 OS=Dictyostelium discoideum GN=DDB_G0270946 PE=3 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 6.1e-12
Identity = 61/232 (26.29%), Postives = 105/232 (45.26%), Query Frame = 1


Query: 11  GTFVPIFVYWFYSGLYLLLG----FFDNYRLHSKTDEDEKNLVSKSTVVRGVLFQQFIQA 70
           GTF+   V++F   +  L+     FF  Y++    + D K+       ++ +L Q  IQ 
Sbjct: 29  GTFIAHQVFYFGCFIPFLIADFIPFFRKYKIQQTKENDWKS--QTYCAIKVILTQVLIQL 88

Query: 71  IVAIIL---FKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYRH 130
            +  I     K  G    + A   S P ++L  +++  I D + Y+AHR +HH    Y++
Sbjct: 89  PMMYIFDPAIKAIGLS--ARAPLPSIPYLLLTLVSSFIIEDFYFYWAHRALHHG-IWYKY 148

Query: 131 IHSQHHRLVVPYAFGALYNHPVEGLLL--DTIGGALSFLASGMTPRISIFFFSFATIKTV 190
           IH  HH    P+   A Y HP+E ++L   T+ G   F     T  +   +      +TV
Sbjct: 149 IHKVHHDYASPFGITAEYAHPLETIILGVGTVIGPFLFSRDLFTLWV---WLGVRLYQTV 208

Query: 191 DDHCGLWLPGNLFHIL-FRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGT 233
           + H G   P +  +++ F     +HD HH+++   +  +   F   D+I GT
Sbjct: 209 ECHSGYDFPWSFTNLIPFWGGAPFHDYHHEVFIGNYAST---FTYLDKIFGT 249

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BLAST of Cucsa.189580 vs. TrEMBL
Match: A0A0A0LJX9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G101090 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.7e-122
Identity = 222/253 (87.75%), Postives = 222/253 (87.75%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLL                               DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLL-------------------------------DEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 222

Query: 241 RASGGFEARPKIE 254
           RASGGFEARPKIE
Sbjct: 241 RASGGFEARPKIE 222

[Close]

BLAST of Cucsa.189580 vs. TrEMBL
Match: M5XMN9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010258mg PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 8.9e-119
Identity = 200/251 (79.68%), Postives = 223/251 (88.84%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGTFVP+ +YW YSG+Y++L  F+NYRLH++ DEDEKNLVSK TVV+GVL 
Sbjct: 1   MGFEVSDELLGTFVPVLIYWAYSGIYVVLDSFENYRLHTRKDEDEKNLVSKRTVVKGVLL 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQ IQAIVAI+LF +TGNDDG + V  S+  +  QF+TAM +LDTWQYF HRYMHHNKFL
Sbjct: 61  QQTIQAIVAILLFTVTGNDDGDSGVKPSFIDLARQFVTAMIVLDTWQYFMHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           Y+HIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFL SGM+PR SIFFFSFATIKT
Sbjct: 121 YKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLLSGMSPRASIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+ F NNTAYHDVHHQLYGSK+NFSQPFFV WDRILGT+MPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFSNNTAYHDVHHQLYGSKYNFSQPFFVMWDRILGTHMPYSLEK 240

Query: 241 RASGGFEARPK 252
           RA GGFE RPK
Sbjct: 241 RAGGGFEVRPK 251

[Close]

BLAST of Cucsa.189580 vs. TrEMBL
Match: A0A0A0KQL6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G605020 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 8.9e-119
Identity = 202/251 (80.48%), Postives = 220/251 (87.65%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M   ISD +LGTFVPI VYW YSGLY+LLG F+NYRLHSK DE EKNLVSKSTVVRGV  
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQ IQAIVAI+LFK+T  D      PKSW  +V+QF+ AM +LDTWQYF HRYMH NKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           Y+H+HSQHHRLVVPYAFGALYNHP+EGLLLDTIGGALSFL SGM+PR++IFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+ FRNN+AYHDVHHQLYGSK+NFSQPFFVTWDRI GTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RASGGFEARPK 252
           RA GGFEAR K
Sbjct: 241 RAGGGFEARVK 251

[Close]

BLAST of Cucsa.189580 vs. TrEMBL
Match: B9S8V4_RICCO (Sur2 hydroxylase/desaturase, putative OS=Ricinus communis GN=RCOM_0836090 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-118
Identity = 204/255 (80.00%), Postives = 227/255 (89.02%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEF ISD+LLGTF+PI +YW YSGLY+ LG+FDNYRLH+K DEDEKNLVSKSTVV+GVL 
Sbjct: 1   MEFQISDELLGTFLPIVIYWVYSGLYIGLGYFDNYRLHTKQDEDEKNLVSKSTVVKGVLL 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVL--QFLTAMFILDTWQYFAHRYMHHNK 120
           QQ IQA+VAIILF +TG DD   A+ +   +++L  QF+TAM +LDTWQYF HRYMHHNK
Sbjct: 61  QQAIQAVVAIILFTVTG-DDSETAMDQKPSLMILARQFVTAMLVLDTWQYFMHRYMHHNK 120

Query: 121 FLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATI 180
           FLY+HIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFL SGM+PR S+FFFSFATI
Sbjct: 121 FLYKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLLSGMSPRASVFFFSFATI 180

Query: 181 KTVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSL 240
           KTVDDHCGLWLPGNLFH+ F+NNTAYHDVHHQLYGSK+NFSQPFFV WD+ILGTYMPYSL
Sbjct: 181 KTVDDHCGLWLPGNLFHVFFKNNTAYHDVHHQLYGSKYNFSQPFFVMWDKILGTYMPYSL 240

Query: 241 EKRASGGFEARPKIE 254
           EKR  GGFEARP  E
Sbjct: 241 EKRDGGGFEARPAKE 254

[Close]

BLAST of Cucsa.189580 vs. TrEMBL
Match: A0A067LAS4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15596 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.0e-118
Identity = 202/254 (79.53%), Postives = 224/254 (88.19%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+LLGTF PI VYW YSGLY+LLGFFDNYRLH+K DEDEKNLVSKSTV +GVL 
Sbjct: 1   MGFQISDELLGTFAPIVVYWVYSGLYILLGFFDNYRLHTKQDEDEKNLVSKSTVFKGVLL 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPK-SWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ IQA+VAI+LF +TGND  ++  P+ S  ++  QF+TAM ++DTWQYF HRYMHHNKF
Sbjct: 61  QQSIQAVVAILLFTVTGNDSQADTYPQPSLIVLARQFVTAMLVMDTWQYFMHRYMHHNKF 120

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRLVVPYAFGALYNHP+EGLLLDTIGGALSFL SGM+PR SIFFFSFATIK
Sbjct: 121 LYKHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLMSGMSPRSSIFFFSFATIK 180

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH+ F+NNTAYHDVHHQLYGSK+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 181 TVDDHCGLWLPGNLFHVFFKNNTAYHDVHHQLYGSKYNFSQPFFVMWDRILGTYMPYSLE 240

Query: 241 KRASGGFEARPKIE 254
           K+  GGFE RP  E
Sbjct: 241 KKEGGGFEVRPTKE 254

[Close]

BLAST of Cucsa.189580 vs. TAIR10
Match: AT1G69640.1 (AT1G69640.1 sphingoid base hydroxylase 1)

HSP 1 Score: 409.1 bits (1050), Expect = 2.0e-114
Identity = 188/254 (74.02%), Postives = 220/254 (86.61%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGT  PI VYW YSG+Y+ L   ++YRLHSK +E+EKNLVSKS+VV+GVL 
Sbjct: 3   MGFAVSDELLGTVAPIVVYWLYSGIYVALSSLESYRLHSKVEEEEKNLVSKSSVVKGVLV 62

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QA+VAI+LF +TG+D +   A   S+ ++  QF+TAM +LDTWQYF HRYMH NKF
Sbjct: 63  QQVVQAVVAILLFTVTGSDAEADKAQQFSFLVLARQFVTAMIVLDTWQYFMHRYMHQNKF 122

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPYA+GALYNHPVEGLLLDTIGGALSFL SGM+PR SIFFFSFATIK
Sbjct: 123 LYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIK 182

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGNLFH++F+NN+AYHD+HHQLYG+K+NFSQPFFV WDRILGTYMPYSLE
Sbjct: 183 TVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQPFFVMWDRILGTYMPYSLE 242

Query: 241 KRASGGFEARPKIE 254
           KR  GGFEARP  E
Sbjct: 243 KREDGGFEARPTKE 256

[Close]

BLAST of Cucsa.189580 vs. TAIR10
Match: AT1G14290.1 (AT1G14290.1 sphingoid base hydroxylase 2)

HSP 1 Score: 408.3 bits (1048), Expect = 3.5e-114
Identity = 188/251 (74.90%), Postives = 216/251 (86.06%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F ISD+ LGTFVPI VYW YSG+Y+ LG  D YRLHSK DEDEKNLVSKS VV+GVL 
Sbjct: 2   MSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLL 61

Query: 61  QQFIQAIVAIILFKLTGND-DGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKF 120
           QQ +QAI+++ILFK+TG+D D +     S  ++  QF+ AM ++DTWQYF HRYMH NKF
Sbjct: 62  QQTLQAIISVILFKITGSDADAATTQQFSILLLARQFIIAMLVIDTWQYFIHRYMHLNKF 121

Query: 121 LYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIK 180
           LY+HIHSQHHRL+VPY++GALYNHP+EGLLLDTIGGALSFL SGM+PR +IFFFSFATIK
Sbjct: 122 LYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFATIK 181

Query: 181 TVDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLE 240
           TVDDHCGLWLPGN FHI F NN+AYHDVHHQLYG+K+NFSQPFFV WDRILGTY+PYSLE
Sbjct: 182 TVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTKYNFSQPFFVMWDRILGTYLPYSLE 241

Query: 241 KRASGGFEARP 251
           KRA+GGFE RP
Sbjct: 242 KRANGGFETRP 252

[Close]

BLAST of Cucsa.189580 vs. TAIR10
Match: AT3G02590.1 (AT3G02590.1 Fatty acid hydroxylase superfamily protein)

HSP 1 Score: 52.0 bits (123), Expect = 6.3e-07
Identity = 39/157 (24.84%), Postives = 71/157 (45.22%), Query Frame = 1


Query: 88  SWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFLYRHIHSQHH---RLVVPYAFGALYNHP 147
           +W +  L     + +++   Y+ H+ +H  KFLY+H+H+ HH   +      F  L  HP
Sbjct: 125 NWFLCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHP 184

Query: 148 VEGLLLDTIGG-ALSFLASGMTPRISIFFFSFATIKTVDDHCGLWLPGNLFHILFRNNTA 207
           ++G+L       AL  +   +   +S+ F       ++ D     + GN++ I+      
Sbjct: 185 LDGILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHD----CIHGNIWPIM---GAG 244

Query: 208 YHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 241
           YH +HH  Y  K N+   + +  D + G+ M    EK
Sbjct: 245 YHTIHHTTY--KHNYGH-YTIWMDWMFGSLMVPLAEK 271

[Close]

BLAST of Cucsa.189580 vs. NCBI nr
Match: gi|449442297|ref|XP_004138918.1| (PREDICTED: sphinganine C(4)-monooxygenase 1-like [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 2.2e-147
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RASGGFEARPKIE
Sbjct: 241 RASGGFEARPKIE 253

[Close]

BLAST of Cucsa.189580 vs. NCBI nr
Match: gi|659082180|ref|XP_008441706.1| (PREDICTED: sphinganine C(4)-monooxygenase 1-like [Cucumis melo])

HSP 1 Score: 525.8 bits (1353), Expect = 4.2e-146
Identity = 247/253 (97.63%), Postives = 252/253 (99.60%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFK+TGNDDGSNAVPKSWPMVVLQFLTAMF+LDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKVTGNDDGSNAVPKSWPMVVLQFLTAMFVLDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDT+GGALSFLASGMTPR+SIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTVGGALSFLASGMTPRVSIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+LFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVLFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240

Query: 241 RASGGFEARPKIE 254
           RA GGFEARPKIE
Sbjct: 241 RAGGGFEARPKIE 253

[Close]

BLAST of Cucsa.189580 vs. NCBI nr
Match: gi|700206260|gb|KGN61379.1| (hypothetical protein Csa_2G101090 [Cucumis sativus])

HSP 1 Score: 446.8 bits (1148), Expect = 2.5e-122
Identity = 222/253 (87.75%), Postives = 222/253 (87.75%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           MEFTISDDLL                               DEDEKNLVSKSTVVRGVLF
Sbjct: 1   MEFTISDDLL-------------------------------DEDEKNLVSKSTVVRGVLF 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL
Sbjct: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT
Sbjct: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 222

Query: 241 RASGGFEARPKIE 254
           RASGGFEARPKIE
Sbjct: 241 RASGGFEARPKIE 222

[Close]

BLAST of Cucsa.189580 vs. NCBI nr
Match: gi|449435354|ref|XP_004135460.1| (PREDICTED: sphinganine C(4)-monooxygenase 2 [Cucumis sativus])

HSP 1 Score: 434.5 bits (1116), Expect = 1.3e-118
Identity = 202/251 (80.48%), Postives = 220/251 (87.65%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M   ISD +LGTFVPI VYW YSGLY+LLG F+NYRLHSK DE EKNLVSKSTVVRGV  
Sbjct: 1   MGIVISDQILGTFVPIVVYWLYSGLYILLGSFENYRLHSKKDELEKNLVSKSTVVRGVFL 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQ IQAIVAI+LFK+T  D      PKSW  +V+QF+ AM +LDTWQYF HRYMH NKFL
Sbjct: 61  QQIIQAIVAILLFKVTETDGEVATGPKSWLTIVVQFIVAMLVLDTWQYFIHRYMHQNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           Y+H+HSQHHRLVVPYAFGALYNHP+EGLLLDTIGGALSFL SGM+PR++IFFFSFATIKT
Sbjct: 121 YKHVHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLVSGMSPRVAIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+ FRNN+AYHDVHHQLYGSK+NFSQPFFVTWDRI GTYMPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFRNNSAYHDVHHQLYGSKYNFSQPFFVTWDRIFGTYMPYSLEK 240

Query: 241 RASGGFEARPK 252
           RA GGFEAR K
Sbjct: 241 RAGGGFEARVK 251

[Close]

BLAST of Cucsa.189580 vs. NCBI nr
Match: gi|596288798|ref|XP_007225995.1| (hypothetical protein PRUPE_ppa010258mg [Prunus persica])

HSP 1 Score: 434.5 bits (1116), Expect = 1.3e-118
Identity = 200/251 (79.68%), Postives = 223/251 (88.84%), Query Frame = 1


Query: 1   MEFTISDDLLGTFVPIFVYWFYSGLYLLLGFFDNYRLHSKTDEDEKNLVSKSTVVRGVLF 60
           M F +SD+LLGTFVP+ +YW YSG+Y++L  F+NYRLH++ DEDEKNLVSK TVV+GVL 
Sbjct: 1   MGFEVSDELLGTFVPVLIYWAYSGIYVVLDSFENYRLHTRKDEDEKNLVSKRTVVKGVLL 60

Query: 61  QQFIQAIVAIILFKLTGNDDGSNAVPKSWPMVVLQFLTAMFILDTWQYFAHRYMHHNKFL 120
           QQ IQAIVAI+LF +TGNDDG + V  S+  +  QF+TAM +LDTWQYF HRYMHHNKFL
Sbjct: 61  QQTIQAIVAILLFTVTGNDDGDSGVKPSFIDLARQFVTAMIVLDTWQYFMHRYMHHNKFL 120

Query: 121 YRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLASGMTPRISIFFFSFATIKT 180
           Y+HIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFL SGM+PR SIFFFSFATIKT
Sbjct: 121 YKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLLSGMSPRASIFFFSFATIKT 180

Query: 181 VDDHCGLWLPGNLFHILFRNNTAYHDVHHQLYGSKFNFSQPFFVTWDRILGTYMPYSLEK 240
           VDDHCGLWLPGNLFH+ F NNTAYHDVHHQLYGSK+NFSQPFFV WDRILGT+MPYSLEK
Sbjct: 181 VDDHCGLWLPGNLFHVFFSNNTAYHDVHHQLYGSKYNFSQPFFVMWDRILGTHMPYSLEK 240

Query: 241 RASGGFEARPK 252
           RA GGFE RPK
Sbjct: 241 RAGGGFEVRPK 251

The following BLAST results are available for this feature:

BLAST of Cucsa.189580 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SBH1_ARATH	3.6e-113	74.02	Sphinganine C4-monooxygenase 1 OS=Arabidopsis thaliana GN=SBH1 PE=1 SV=1	[more]
SBH2_ARATH	6.2e-113	74.90	Sphinganine C4-monooxygenase 2 OS=Arabidopsis thaliana GN=SBH2 PE=1 SV=1	[more]
SUR2_SCHPO	1.1e-56	43.08	Sphingolipid C4-hydroxylase sur2 OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]
SUR2_YEAST	4.3e-50	40.48	Sphingolipid C4-hydroxylase SUR2 OS=Saccharomyces cerevisiae (strain ATCC 204508...	[more]
MSMOA_DICDI	6.1e-12	26.29	Putative methylsterol monooxygenase DDB_G0270946 OS=Dictyostelium discoideum GN=...	[more]

BLAST of Cucsa.189580 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJX9_CUCSA	1.7e-122	87.75	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G101090 PE=3 SV=1	[more]
M5XMN9_PRUPE	8.9e-119	79.68	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa010258mg PE=3 SV=1	[more]
A0A0A0KQL6_CUCSA	8.9e-119	80.48	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G605020 PE=3 SV=1	[more]
B9S8V4_RICCO	1.2e-118	80.00	Sur2 hydroxylase/desaturase, putative OS=Ricinus communis GN=RCOM_0836090 PE=3 S...	[more]
A0A067LAS4_JATCU	2.0e-118	79.53	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_15596 PE=3 SV=1	[more]

BLAST of Cucsa.189580 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G69640.1	2.0e-114	74.02	sphingoid base hydroxylase 1	[more]
AT1G14290.1	3.5e-114	74.90	sphingoid base hydroxylase 2	[more]
AT3G02590.1	6.3e-07	24.84	Fatty acid hydroxylase superfamily protein	[more]

BLAST of Cucsa.189580 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442297\|ref\|XP_004138918.1\|	2.2e-147	100.00	PREDICTED: sphinganine C(4)-monooxygenase 1-like [Cucumis sativus]	[more]
gi\|659082180\|ref\|XP_008441706.1\|	4.2e-146	97.63	PREDICTED: sphinganine C(4)-monooxygenase 1-like [Cucumis melo]	[more]
gi\|700206260\|gb\|KGN61379.1\|	2.5e-122	87.75	hypothetical protein Csa_2G101090 [Cucumis sativus]	[more]
gi\|449435354\|ref\|XP_004135460.1\|	1.3e-118	80.48	PREDICTED: sphinganine C(4)-monooxygenase 2 [Cucumis sativus]	[more]
gi\|596288798\|ref\|XP_007225995.1\|	1.3e-118	79.68	hypothetical protein PRUPE_ppa010258mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006694	Fatty_acid_hydroxylase

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006633	fatty acid biosynthetic process
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0000254	C-4 methylsterol oxidase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.189580.1	Cucsa.189580.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 96..232 score: 3.2
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 5..250 score: 1.2E
None	No IPR available	PANTHER	PTHR11863:SF43	SUBFAMILY NOT NAMED	coord: 5..250 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.189580	Cla004167	Watermelon (97103) v1	cgywmB368
Cucsa.189580	Csa2G101090	Cucumber (Chinese Long) v2	cgycuB292
Cucsa.189580	MELO3C008543	Melon (DHL92) v3.5.1	cgymeB345
Cucsa.189580	ClCG11G009940	Watermelon (Charleston Gray)	cgywcgB346
Cucsa.189580	CSPI02G08280	Wild cucumber (PI 183967)	cgycpiB301
Cucsa.189580	CmaCh01G004730	Cucurbita maxima (Rimu)	cgycmaB0552
Cucsa.189580	CmaCh05G008580	Cucurbita maxima (Rimu)	cgycmaB0553
Cucsa.189580	CmoCh01G005070	Cucurbita moschata (Rifu)	cgycmoB0553
Cucsa.189580	Lsi11G009750	Bottle gourd (USVL1VR-Ls)	cgylsiB324
Cucsa.189580	Cp4.1LG11g07210	Cucurbita pepo (Zucchini)	cgycpeB0533
Cucsa.189580	Cp4.1LG02g12330	Cucurbita pepo (Zucchini)	cgycpeB0534
Cucsa.189580	MELO3C008543.2	Melon (DHL92) v3.6.1	cgymedB340
Cucsa.189580	CsaV3_2G010670	Cucumber (Chinese Long) v3	cgycucB304
Cucsa.189580	Cla97C11G216250	Watermelon (97103) v2	cgywmbB352
Cucsa.189580	Bhi06G000767	Wax gourd	cgywgoB394
Cucsa.189580	Carg20419	Silver-seed gourd	carcgyB0782

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None