Cucsa.178810 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.178810
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	General transcription factor IIH subunit 3
Location	scaffold01227 : 1164629 .. 1169022 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCATTCTGAAGCAGCCATGGCTTCAGCTCCTTCGAAGCTTTACGCAGGTTCTCCTCCAATCCCTTTCTCCTTCCTTATGGGCACTCGGGTACAACTATCGAAATTGCAGTGGCTTATTCGTTTTTTtGATCTTTGATTGTGTTGTTGCAGATGATGTTAGCCTTTTAGTGGTTTTACTGGATACGAATCCATTTTTCTGGAGCACATCTGCTCTTCCGTTCTCCAAGTTTCTGTCTCATGTAATTGATAAATTATCTCCTTTCCTGTGAACCATTCTACCCAGTAGGTTTTTCTGATAGAACTGTAATGAACATTAGCATTTCCTTTTTCTTTCTGTTTTGGGTGTATAGGTACTTGCTTTTCTGAACTCCATTTTAGTTCTGAACCAACTTAATGAGGTTGTGGTTATTGGTACTGGATATGCTTCATGCAAGTATTTATACAACTCGTCTTCTTACTCAAATCATGGCCTTGAAGATGGTAGAATGCCTGCACTTTGTACTCGTTTATTGAAGAATTTGGAGGAGTTCGTGATTGGGGATGAGCAGTCCATCAAGGAAGATCCCAAAGGAGGGACCATGTCTTCACTTCTTTCTGGATCGCTCTCCATGGCCTTGTGTTGTATCCTTCCTTGTTGATGATTATTGGTGCTGATTATTTGCTTGTTTATGCTCATAAGTAAAGCTTGACATGTTCTACAAATGGGCCATTAATGTAGCAGTCAGGACTAAACAGTATCTATTGTGATGGGAGTGGCATTTGGTCGAAATTTGTGGTAATGAGTATGGTTGCAAAATGTTGTAAGAGTTATGGTTGGCACGATGGAATTTTCTAATAGTCACACTGTTTAAATGTAACTTGAGTAATGTAATATTCATCAACATACTGTTATGCTTCAAATGCTTGACCCTGTGATTAGATATACAGAAAGTTTTCCGCTCTGGATCTCTCCATCCCCAACCACGAGTAAGCATTTGTTTCTGTTCTGGATAATTTATTCCCAATTTGCATATCCTGATTTTTCTTGTTGCTTACATATACTATTTGTTTTTGTCCTTTTTTGAAAATGTGACTAATCTATGTGAATATATTTTACTTTGAGTGTAGATCCTTTGCTTGCAGGGATCCCCAGATGGTCCTGAACAGTAAGAATATTTTCTTTATTCTTTAAAAATATGTATATATTTCTAATGAAGTTATTTTATTTGTGCAATTTTCTTTAATAATGGGAATCAAACTTTAAAATCTTCCGTCTAGTGACTGTTTTTCTCTTCCAGATATGTTGCAATCATGAATGCCATCTTTTCTGCTCAGCGTTCAATGGTTTGTATTATTTTGTTCACTCTTCTGTGTGTTCTTATATGACTTTACTAACTTCTGTCAATCACCAACTCTCATTATTTGAACTGGGTGATATCAGTAAGTAATGAAAATGTGACCACAAGTATTTCATGGAAATGCTCGAGACAAGTTTCCTCTCTATTTAACTTGTTCTTTGTTTGGTCTTGATGGCAGAAAATGGTGCTGGATTAAACTTTTTTGTTCGTTTCTTTTCCTATTTGAATTTGAAGATATCAATTTTTTCTCCCCCTATTTCGAGATATCCCACTTGTCCCATAGGCGAATTTGAATTTGAATGCATTAACAAGACTAAGTATTTTATATTGAACTACTTTCTTGGAGTTTACAACTGAATATCTAGTCTCTACCGTTAGAGTTGAACTCCAATATTTGGTATAGATTTTATTATTTAATTTGCTTGCCCTGTGGATGGAAAGCTGATGGATCTCTGGCATCTTTTCTACTATTAGTCTAGTCAGATTCAGTTGTGAAAGTTTTCATGTTTATATGTATGCATGTAAGTGTATGTATAAAGAATAAGAAGAAGAACAAATCTAGAGAGCAAAGATTCTTCGAAAAGGACAAGGGGAGAATTGGGAGTTTCCCTGAAGGAAGATTCTGGGATAGAAGTTTATAAGAAAAACTTCAGTCCAAGTTATCGATAGAGTAGAGAAGTAGCAAAACCCTTATGTAAGGTACTCCAAGTAAAGGCTGTAACTGTACAAGCTCCAAAATTTAGGAGAACAGTCCTGAAAAAACCAATAAAGAGTTGTATTTTCAAATTGGAAAAATTATAAATAGAGATGAACTTTTAATTTACTCTCTCCAACAACACATCAAATCCAAAGGTGAAAACTACTTAAGATTTTTGAATTGCTAGTTTTCATTTTGGTTATGACAGCTTAACGTAACAATATTGACCAAAGTATGATTAATTTCTTGATATCAAGTAATTGCATCTGCAATGTTCATGATAATAGAAATCATTTGGTCACAAGCAGAAAGTGGTAGTCAATGAGATCGTAAAAATGTGAATCTAATCTTCATTCACATTTGCATTTATGGGTTATGTTTAAACTTTCATGTGTGCTAGTCACTCATTTGAAGTTCACAGCTGTCTCCGTTTTCCTCTCCATGTTGCTTTTAACTATAAACTACATTCAACATTGAACAATGTTTTCATTTGTATAAGTATCCTATGATGTACAATTTTTCCTATGATGTACAATTTTATCATTCTTGCTTCAGGTTCCTATAGATTCATGTTACATTGGTTCCCACAATTCTGCATTTCTTCAGCAGGTAAGCTAACTGCTCATTGTTCCTTAATGGGTTTTTtACTTTtCTTGGAATCTTACAGTTACCATCTCCGGATGTATCATCTATTCATTGCTGGTGATGATTTGATTTCTGATGCTGTATAGGCTTCTTACATAACGGGTGGAGTTTATCTGAAGCCTCAGCAAATGGATGGGCTGTTTCAGTATCTCTCTGTAAGTTTTACTTTCCTATGGGTTCCCcTGTTTTTtGTAATCTCTTGCTTAGGCTGTTGTGATATTCTATTGTTCTTTCTATGCAGACTGTTTTTGGCACCGATTTGCATTCCCGGACCTTTTTACAGCTTCCAAAATCTGTTGGTGTGGATTTTCGTGCATCGTAAGTCTGCAGTCTTGTGAATACAAAATTCTGTTGTTTTGTGCTTATTATTTTGTGTCACTTACTACGGCCTTCTACAGATTTACACCTCTCATCATCATTTTGGGAATTTGCAGGTGTTTTTGCCACAAGAAAACGATCGATATGGGCTATGTCTGTTCTGTTTGTTTATCTATATTCTGCAAGCATCACAAGAAATGTTCTACCTGTGGGTAAGCGAAAGGTCAAGCCATAATTATTATGTTTTTCCCTTTTACTTGTGGTTTCCTAAAATTTATTGAAGGTAGTTTGACATTGACTTTCGAGAATGCTGTTGTCTGTTTGGCTTTATAAGTATGAAAaGGAAGATGAAGAGGAACCTAATTTTTGAGAACTCATTATGCTAGGGCTTTTTCCTCGGCAGTTTATATAATATTTCGGTGAGACATGATGTCAACGCGAGGGGGAGCTTTGTACCTTAGTATTGCCCCTAGATAATGGTTGCCTAAATAAGATGCTAAAAAATTCTGCCACGGCTTAAGTTTCTTAGGGGTGTTTGGAAAACTGAAATGTAATTAGAGTCACTTTGAATCAGAGTGGTGTTAAAATGGGTGTTGAATTGAGGGCAAAACAAAAATGGGGTCGTAAAGCGCCAAGACCCTTCTGAAATGCTAAAAAAACTCAACAATGTTGTTTGCCTACTATTACTTCTGTCTACTTCAGAACAAGATTGAATGTATTAATATTAATGAGTTTCAACTTCATTCAGGTCAGTTTTTGGTGAGACACCAGTAGAACTCGATTCAGTGTCTAAACTGAAGAGAAAAACTCCAGAATGATTGCGCTCTTCTGTAAGTTGTGATGGATCATTTCATGAAACTCAGTGTTATTTTATGATTTTGAACCATTTGTTTTCACTTCCATGGCTATAGAACTGCTTCATTTTTCTTCCCTTTCTTGAAGCTGTTTGGAACTGGAATTCATTTGGTTCACATGGCATGAACAGAGATGACTCAAAGGAAACAGTTGCTGAAAGAGAGTCAATCTCAACATTGTACAATTCAGATCAATAGTTGTCGAATTCGGATGGGATAACATGGAACGATGGCTAGCAAGGTCATGCATCTGTATTTCGTCTATTCCAGTCTGATACAAATCGGGTACAAGGTTTATATGGAATTCGAGACTTTAACTAGAAAAGTTAGTCTAATGTAATTGTACCGAAAGCTTGAGTGGTTTATAAGTACATGTAGATTTATATTTCTCTCACGCTTGAAAGTACTCCAAAGCTTACCTTTGTAAAGGTAGACAGAAGGATAGGGATATTCACATTGTTGATTTCAAGACCTCATACTTGGAAATTTTACGAGTTTGAAATGTGCCAAGTGAATCATATAATACTACAATTTGAGCATA

mRNA sequence

GCCCATTCTGAAGCAGCCATGGCTTCAGCTCCTTCGAAGCTTTACGCAGATGATGTTAGCCTTTTAGTGGTTTTACTGGATACGAATCCATTTTTCTGGAGCACATCTGCTCTTCCGTTCTCCAAGTTTCTGTCTCATGTACTTGCTTTTCTGAACTCCATTTTAGTTCTGAACCAACTTAATGAGGTTGTGGTTATTGGTACTGGATATGCTTCATGCAAGTATTTATACAACTCGTCTTCTTACTCAAATCATGGCCTTGAAGATGGTAGAATGCCTGCACTTTGTACTCGTTTATTGAAGAATTTGGAGGAGTTCGTGATTGGGGATGAGCAGTCCATCAAGGAAGATCCCAAAGGAGGGACCATGTCTTCACTTCTTTCTGGATCGCTCTCCATGGCCTTGTGTTATATACAGAAAGTTTTCCGCTCTGGATCTCTCCATCCCCAACCACGAATCCTTTGCTTGCAGGGATCCCCAGATGGTCCTGAACAATATGTTGCAATCATGAATGCCATCTTTTCTGCTCAGCGTTCAATGGTTCCTATAGATTCATGTTACATTGGTTCCCACAATTCTGCATTTCTTCAGCAGGCTTCTTACATAACGGGTGGAGTTTATCTGAAGCCTCAGCAAATGGATGGGCTGTTTCAGTATCTCTCTACTGTTTTTGGCACCGATTTGCATTCCCGGACCTTTTTACAGCTTCCAAAATCTGTTGGTGTGGATTTTCGTGCATCGTGTTTTTGCCACAAGAAAACGATCGATATGGGCTATGTCTGTTCTGTTTGTTTATCTATATTCTGCAAGCATCACAAGAAATGTTCTACCTGTGGGTCAGTTTTTGGTGAGACACCAGTAGAACTCGATTCAGTGTCTAAACTGAAGAGAAAAACTCCAGAATGATTGCGCTCTTCTCTGTTTGGAACTGGAATTCATTTGGTTCACATGGCATGAACAGAGATGACTCAAAGGAAACAGTTGCTGAAAGAGAGTCAATCTCAACATTGTACAATTCAGATCAATAGTTGTCGAATTCGGATGGGATAACATGGAACGATGGCTAGCAAGGTCATGCATCTGTATTTCGTCTATTCCAGTCTGATACAAATCGGGTACAAGGTTTATATGGAATTCGAGACTTTAACTAGAAAAGTTAGTCTAATGTAATTGTACCGAAAGCTTGAGTGGTTTATAAGTACATGTAGATTTATATTTCTCTCACGCTTGAAAGTACTCCAAAGCTTACCTTTGTAAAGGTAGACAGAAGGATAGGGATATTCACATTGTTGATTTCAAGACCTCATACTTGGAAATTTTACGAGTTTGAAATGTGCCAAGTGAATCATATAATACTACAATTTGAGCATA

Coding sequence (CDS)

ATGGCTTCAGCTCCTTCGAAGCTTTACGCAGATGATGTTAGCCTTTTAGTGGTTTTACTGGATACGAATCCATTTTTCTGGAGCACATCTGCTCTTCCGTTCTCCAAGTTTCTGTCTCATGTACTTGCTTTTCTGAACTCCATTTTAGTTCTGAACCAACTTAATGAGGTTGTGGTTATTGGTACTGGATATGCTTCATGCAAGTATTTATACAACTCGTCTTCTTACTCAAATCATGGCCTTGAAGATGGTAGAATGCCTGCACTTTGTACTCGTTTATTGAAGAATTTGGAGGAGTTCGTGATTGGGGATGAGCAGTCCATCAAGGAAGATCCCAAAGGAGGGACCATGTCTTCACTTCTTTCTGGATCGCTCTCCATGGCCTTGTGTTATATACAGAAAGTTTTCCGCTCTGGATCTCTCCATCCCCAACCACGAATCCTTTGCTTGCAGGGATCCCCAGATGGTCCTGAACAATATGTTGCAATCATGAATGCCATCTTTTCTGCTCAGCGTTCAATGGTTCCTATAGATTCATGTTACATTGGTTCCCACAATTCTGCATTTCTTCAGCAGGCTTCTTACATAACGGGTGGAGTTTATCTGAAGCCTCAGCAAATGGATGGGCTGTTTCAGTATCTCTCTACTGTTTTTGGCACCGATTTGCATTCCCGGACCTTTTTACAGCTTCCAAAATCTGTTGGTGTGGATTTTCGTGCATCGTGTTTTTGCCACAAGAAAACGATCGATATGGGCTATGTCTGTTCTGTTTGTTTATCTATATTCTGCAAGCATCACAAGAAATGTTCTACCTGTGGGTCAGTTTTTGGTGAGACACCAGTAGAACTCGATTCAGTGTCTAAACTGAAGAGAAAAACTCCAGAATGA

Protein sequence

MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSLLSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSCYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE*

Homology

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BLAST of Cucsa.178810 vs. Swiss-Prot
Match: TFB4_ARATH (RNA polymerase II transcription factor B subunit 4 OS=Arabidopsis thaliana GN=TFB4 PE=2 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 4.6e-120
Identity = 215/299 (71.91%), Postives = 249/299 (83.28%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           M +  SK Y+DDVSLLV+LLDTNP FWST+++ FS+FLSHVLAFLN++L LNQLN+VVVI
Sbjct: 1   MPAIASKQYSDDVSLLVLLLDTNPLFWSTTSITFSQFLSHVLAFLNAVLGLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGR---MPALCTRLLKNLEEFVIGDEQSIKEDPKGGTM 120
            TGY+SC Y+Y+SS  SNHG  +     MPA+   LLK LEEFV  DE+  KE+     +
Sbjct: 61  ATGYSSCDYIYDSSLTSNHGNFESNGTGMPAIFGSLLKKLEEFVTKDEELSKEEVSEDRI 120

Query: 121 SS-LLSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVP 180
            S LLSGSLSMALCYIQ+VFRSG LHPQPRILCLQGSPDGPEQYVA+MN+IFSAQR MVP
Sbjct: 121 PSCLLSGSLSMALCYIQRVFRSGHLHPQPRILCLQGSPDGPEQYVAVMNSIFSAQRLMVP 180

Query: 181 IDSCYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGV 240
           IDSCYIG  NSAFLQQASYITGGV+  P+Q+DGLFQYL+T+F TDLHSR F+QLPK +GV
Sbjct: 181 IDSCYIGVQNSAFLQQASYITGGVHHTPKQLDGLFQYLTTIFATDLHSRGFVQLPKPIGV 240

Query: 241 DFRASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPV-ELDSVSKLKRKTP 295
           DFRASCFCHKKTIDMGY+CSVCLSIFC+HHKKCSTCGSVFG++ + +  S S  KRK P
Sbjct: 241 DFRASCFCHKKTIDMGYICSVCLSIFCEHHKKCSTCGSVFGQSKLDDASSASDKKRKAP 299

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BLAST of Cucsa.178810 vs. Swiss-Prot
Match: TF2H3_DICDI (General transcription factor IIH subunit 3 OS=Dictyostelium discoideum GN=gtf2h3 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.1e-49
Identity = 106/255 (41.57%), Postives = 151/255 (59.22%), Query Frame = 1


Query: 34  FSKFLSHVLAFLNSILVLNQLNEVVVIGTGYASCKYLYNSSSYSNHGLEDGRMPA----- 93
           F+KFL H + F+N+ L+LNQ N++ +I +      +++  S+   +  E   +       
Sbjct: 92  FNKFLEHFMVFINAYLMLNQENQLAIICSKIGESSFVFPQSNIDQYQQEQQELEQRQLNE 151

Query: 94  ---LCTRLLKNLEEFVIGDEQS----IKEDPKGGTMSSLLSGSLSMALCYIQKVFRSGSL 153
              L     K ++  ++   Q     IK D +   +SS  S S+S+ALCYI ++ R    
Sbjct: 152 NGELLPTPNKTIQGQILAKLQKLDLEIKHD-QTDILSSSFSASMSIALCYINRIKRETPT 211

Query: 154 HPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSCYIGSHNSAFLQQASYITGGVY 213
             +PRIL    SPD   QY+++MN IFS+Q+  +P+DSC +   +S FLQQAS++T G+Y
Sbjct: 212 I-KPRILVFNISPDVSSQYISVMNCIFSSQKQSIPVDSCILSQSDSTFLQQASHLTSGIY 271

Query: 214 LKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRASCFCHKKTIDMGYVCSVCLSI 273
           LKPQ+ + L QYL T F  D  SR  L  P    VD+RASCFCHK+ +D+GYVCSVCLSI
Sbjct: 272 LKPQKQELLSQYLLTTFLLDTLSRKSLAYPTLKSVDYRASCFCHKRIVDIGYVCSVCLSI 331

Query: 274 FCKHHKKCSTCGSVF 277
           FC H   CSTCG+ F
Sbjct: 332 FCGHSSSCSTCGTKF 344

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BLAST of Cucsa.178810 vs. Swiss-Prot
Match: TF2H3_HUMAN (General transcription factor IIH subunit 3 OS=Homo sapiens GN=GTF2H3 PE=1 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 1.5e-46
Identity = 115/306 (37.58%), Postives = 171/306 (55.88%), Query Frame = 1


Query: 11  DDVSLLVVLLDTNPFFWSTSALPFSKF-----LSHVLAFLNSILVLNQLNEVVVIGTGYA 70
           D+++LLV+++D NP +W   AL  S+F     +  V+   NS L +N+ N++ VI +   
Sbjct: 6   DELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHIQ 65

Query: 71  SCKYLY---------------NSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSI-- 130
             ++LY               N   ++  G +DG+       LL +  E ++ + + +  
Sbjct: 66  ESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKY-----ELLTSANEVIVEEIKDLMT 125

Query: 131 KEDPKGGTMSSLLSGSLSMALCYIQKVFRS--GSLHPQPRILCLQGSPDGPEQYVAIMNA 190
           K D KG    +LL+GSL+ ALCYI ++ +    +   + RIL ++ + D   QY+  MN 
Sbjct: 126 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV 185

Query: 191 IFSAQRSMVPIDSCYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRT 250
           IF+AQ+  + ID+C + S +S  LQQA  ITGG+YLK  QM  L QYL  VF  D   R+
Sbjct: 186 IFAAQKQNILIDACVLDS-DSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRS 245

Query: 251 FLQLPKSVGVDFRASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSV 293
            L LP  V VD+RA+CFCH+  I++GYVCSVCLSIFC     C+TC + F    + L  V
Sbjct: 246 QLILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAF---KISLPPV 302

[Close]

BLAST of Cucsa.178810 vs. Swiss-Prot
Match: TF2H3_BOVIN (General transcription factor IIH subunit 3 OS=Bos taurus GN=GTF2H3 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 4.4e-46
Identity = 116/305 (38.03%), Postives = 168/305 (55.08%), Query Frame = 1


Query: 11  DDVSLLVVLLDTNPFFWSTSALPFSKF-----LSHVLAFLNSILVLNQLNEVVVIGTGYA 70
           D+++LLV+++DTNP +W   AL  S+F     +  V+   NS L +N+ N++ VI +   
Sbjct: 6   DELNLLVIIVDTNPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHIQ 65

Query: 71  SCKYLYN----------------SSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIK 130
             ++LY                 SS ++  G +DG+   L        EE     +   K
Sbjct: 66  ESRFLYPGKNGRLGDFFGDPGNPSSEFTPSGSKDGKYELLTAANEVIAEEI---KDLMTK 125

Query: 131 EDPKGGTMSSLLSGSLSMALCYIQKVFRS--GSLHPQPRILCLQGSPDGPEQYVAIMNAI 190
            D +G    +LL+GSL+ ALCYI ++ +    +   + RIL ++ + D   QY+  MN I
Sbjct: 126 SDIEGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNVI 185

Query: 191 FSAQRSMVPIDSCYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTF 250
           F+AQ+  + ID+C + S +S  LQQA  ITGG+YLK  QM  L QYL  VF  D   R+ 
Sbjct: 186 FAAQKQNILIDACVLDS-DSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ 245

Query: 251 LQLPKSVGVDFRASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVS 293
           L LP  V VD+RA+CFCH+  I++GYVCSVCLSIFC     C+TC + F    + L  V 
Sbjct: 246 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAF---KISLPPVL 303

[Close]

BLAST of Cucsa.178810 vs. Swiss-Prot
Match: TF2H3_MOUSE (General transcription factor IIH subunit 3 OS=Mus musculus GN=Gtf2h3 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-45
Identity = 118/305 (38.69%), Postives = 168/305 (55.08%), Query Frame = 1


Query: 11  DDVSLLVVLLDTNPFFWSTSALPFSKF-----LSHVLAFLNSILVLNQLNEVVVIGTGYA 70
           D+++LLV+++DTNP +W   AL  S+F     +  V+   NS L +N+ N++ VI +   
Sbjct: 6   DELNLLVIIVDTNPIWWGKQALKESQFTLSKCMDAVMVLANSHLFMNRSNQLAVIASHIQ 65

Query: 71  SCKYLYNSSSYSNHGLED-----GRMPALCT---------RLLKNLEEFVIGDEQSI--K 130
             + LY      N GL D     G     C           LL    E +  + + +  K
Sbjct: 66  ESRLLYPGK---NGGLGDFFGDPGNALPDCNPSGSKDGKYELLTVANEVIAEEIKDLMTK 125

Query: 131 EDPKGGTMSSLLSGSLSMALCYIQKVFRS--GSLHPQPRILCLQGSPDGPEQYVAIMNAI 190
            D KG    +LL+GSL+ ALCYI +V ++   +   + RIL ++ + D   QY+  MN I
Sbjct: 126 SDIKGQHTETLLAGSLAKALCYIHRVNKAVKDNQEMKSRILVIKAAEDSALQYMNFMNVI 185

Query: 191 FSAQRSMVPIDSCYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTF 250
           F+AQ+  + ID+C + S +S  LQQA  ITGG+YLK  QM  L QYL  VF  D   R+ 
Sbjct: 186 FAAQKQNILIDACVLDS-DSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ 245

Query: 251 LQLPKSVGVDFRASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVS 293
           L LP  + VD+RA+CFCH+  I++GYVCSVCLSIFC     C+TC + F    + L  V 
Sbjct: 246 LILPPPIHVDYRAACFCHRSLIEIGYVCSVCLSIFCNFSPICTTCETAF---KISLPPVL 303

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BLAST of Cucsa.178810 vs. TrEMBL
Match: A0A0A0LMM2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G072450 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 4.2e-168
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
           GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC
Sbjct: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA
Sbjct: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE
Sbjct: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 295

[Close]

BLAST of Cucsa.178810 vs. TrEMBL
Match: M5XYU6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009386mg PE=4 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.0e-134
Identity = 241/295 (81.69%), Postives = 263/295 (89.15%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLL+VLLDTNPFFWS+S+LPFS FLSHVL FLNSIL+LNQLN+VVVI
Sbjct: 1   MASAPSKLYADDVSLLMVLLDTNPFFWSSSSLPFSVFLSHVLTFLNSILLLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
            TGY SC Y+Y+SS+ +N G ++GRMPA C  LL+ LEEFVI DEQ IKE  + G  SSL
Sbjct: 61  ATGYNSCSYIYDSSTSTNQGSDNGRMPARCVNLLQKLEEFVIEDEQLIKEGLREGIASSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQ+VFRSG LHPQPRILCLQGS DGPEQYVAIMNAIFSAQRS VPIDSC
Sbjct: 121 LSGSLSMALCYIQRVFRSGPLHPQPRILCLQGSSDGPEQYVAIMNAIFSAQRS-VPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           Y+GS NSAFLQQASYITGGVYLKPQQ +GLFQYLSTVF TDLHSR FLQLPKS+GVDFRA
Sbjct: 181 YMGSSNSAFLQQASYITGGVYLKPQQPNGLFQYLSTVFATDLHSRAFLQLPKSLGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHKKTIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+   +++S S  KRKTPE
Sbjct: 241 SCFCHKKTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQSDVNSTSNKKRKTPE 294

[Close]

BLAST of Cucsa.178810 vs. TrEMBL
Match: A0A061FGT5_THECC (Basal transcription factor complex subunit-related isoform 1 OS=Theobroma cacao GN=TCM_035096 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 8.2e-132
Identity = 232/295 (78.64%), Postives = 257/295 (87.12%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSL+VVL+DTNPFFWS S+L FS+FLSHVLAFLN+IL LNQLN+VVVI
Sbjct: 1   MASAPSKLYADDVSLVVVLVDTNPFFWSASSLSFSQFLSHVLAFLNAILTLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
            TGY SC Y+++SSS  N   E+GRMP +C+ LL+ LEEF+I DEQ  KE P+G   SSL
Sbjct: 61  ATGYNSCNYIFDSSSDLNQSFENGRMPVMCSSLLQKLEEFLIKDEQLSKEVPEGRIKSSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQ+VFRSG+LHP PRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC
Sbjct: 121 LSGSLSMALCYIQRVFRSGALHPHPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           Y+G+ NSAFLQQASYITGGV+ KPQ +DGLFQYL T+F TDLHSR+FL LPK VGVDFRA
Sbjct: 181 YMGAQNSAFLQQASYITGGVHHKPQHLDGLFQYLMTIFATDLHSRSFLHLPKPVGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHK TIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+   E  S S  KRKTPE
Sbjct: 241 SCFCHKNTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQSEAASTSDKKRKTPE 295

[Close]

BLAST of Cucsa.178810 vs. TrEMBL
Match: E0CR79_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g03100 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 4.1e-131
Identity = 234/296 (79.05%), Postives = 260/296 (87.84%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MA  PSKLY+DDVSLLVVLLDTNPFFWST++LPFSKFLSHVLAFLNSIL++NQLN+VVVI
Sbjct: 1   MAPVPSKLYSDDVSLLVVLLDTNPFFWSTASLPFSKFLSHVLAFLNSILLINQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSY-SNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSS 120
            TG  SC ++++SSS  +N  LE+GRMPALC+ LL+ LEEFV GDE+  KE    G  SS
Sbjct: 61  ATGCNSCNFIFDSSSVPANPNLENGRMPALCSNLLQKLEEFVTGDEKLSKEVLAAGIGSS 120

Query: 121 LLSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDS 180
           LLSGSLSMALCYIQ+VFR+G LHPQPRILCLQGSPDGPEQYVA+MNAIFSAQRSMVPIDS
Sbjct: 121 LLSGSLSMALCYIQRVFRTGPLHPQPRILCLQGSPDGPEQYVAVMNAIFSAQRSMVPIDS 180

Query: 181 CYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFR 240
           C IG+ +SAFLQQASYITGGVYLKPQQ+DGLFQYLSTVF TDLHSR FLQLPK  GVDFR
Sbjct: 181 CVIGAQHSAFLQQASYITGGVYLKPQQLDGLFQYLSTVFATDLHSRRFLQLPKPAGVDFR 240

Query: 241 ASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           ASCFCHK TIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+   + +S +  KRKTPE
Sbjct: 241 ASCFCHKNTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQSDGNSATDRKRKTPE 296

[Close]

BLAST of Cucsa.178810 vs. TrEMBL
Match: A0A0D2TQJ3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G245300 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 3.5e-130
Identity = 230/295 (77.97%), Postives = 255/295 (86.44%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSL+VVLLDTNPFFWS+S+L FS+FLSHVLAFLN+IL LNQLN+VVVI
Sbjct: 1   MASAPSKLYADDVSLVVVLLDTNPFFWSSSSLSFSQFLSHVLAFLNAILTLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
            TGY SC Y+++SSS  N   E+GRMP +C+ LL+ LEEF+I DEQ  KE+P+G    SL
Sbjct: 61  ATGYNSCDYVFDSSSDLNRSFENGRMPVMCSSLLQKLEEFLITDEQLSKEEPEGKIKPSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
            SGSLSMALCYIQ+VFRSG+LHP PRILCLQGS DGPEQYVAIMNAIFSAQRS VPIDSC
Sbjct: 121 FSGSLSMALCYIQRVFRSGALHPHPRILCLQGSLDGPEQYVAIMNAIFSAQRSSVPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           YIGS NSAFLQQASYITGGV+ KPQ +DGLFQYL T+F TDLHSR+F+ LPK VGVDFRA
Sbjct: 181 YIGSQNSAFLQQASYITGGVHHKPQNLDGLFQYLMTIFATDLHSRSFIHLPKPVGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHK TIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+   E  S S  KRKTPE
Sbjct: 241 SCFCHKNTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQSEAASASDKKRKTPE 295

[Close]

BLAST of Cucsa.178810 vs. TAIR10
Match: AT1G18340.1 (AT1G18340.1 basal transcription factor complex subunit-related)

HSP 1 Score: 432.2 bits (1110), Expect = 2.6e-121
Identity = 215/299 (71.91%), Postives = 249/299 (83.28%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           M +  SK Y+DDVSLLV+LLDTNP FWST+++ FS+FLSHVLAFLN++L LNQLN+VVVI
Sbjct: 1   MPAIASKQYSDDVSLLVLLLDTNPLFWSTTSITFSQFLSHVLAFLNAVLGLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGR---MPALCTRLLKNLEEFVIGDEQSIKEDPKGGTM 120
            TGY+SC Y+Y+SS  SNHG  +     MPA+   LLK LEEFV  DE+  KE+     +
Sbjct: 61  ATGYSSCDYIYDSSLTSNHGNFESNGTGMPAIFGSLLKKLEEFVTKDEELSKEEVSEDRI 120

Query: 121 SS-LLSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVP 180
            S LLSGSLSMALCYIQ+VFRSG LHPQPRILCLQGSPDGPEQYVA+MN+IFSAQR MVP
Sbjct: 121 PSCLLSGSLSMALCYIQRVFRSGHLHPQPRILCLQGSPDGPEQYVAVMNSIFSAQRLMVP 180

Query: 181 IDSCYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGV 240
           IDSCYIG  NSAFLQQASYITGGV+  P+Q+DGLFQYL+T+F TDLHSR F+QLPK +GV
Sbjct: 181 IDSCYIGVQNSAFLQQASYITGGVHHTPKQLDGLFQYLTTIFATDLHSRGFVQLPKPIGV 240

Query: 241 DFRASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPV-ELDSVSKLKRKTP 295
           DFRASCFCHKKTIDMGY+CSVCLSIFC+HHKKCSTCGSVFG++ + +  S S  KRK P
Sbjct: 241 DFRASCFCHKKTIDMGYICSVCLSIFCEHHKKCSTCGSVFGQSKLDDASSASDKKRKAP 299

[Close]

BLAST of Cucsa.178810 vs. NCBI nr
Match: gi|449470273|ref|XP_004152842.1| (PREDICTED: general transcription factor IIH subunit 3 [Cucumis sativus])

HSP 1 Score: 598.6 bits (1542), Expect = 6.0e-168
Identity = 295/295 (100.00%), Postives = 295/295 (100.00%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
           GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC
Sbjct: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA
Sbjct: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE
Sbjct: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 295

[Close]

BLAST of Cucsa.178810 vs. NCBI nr
Match: gi|659082582|ref|XP_008441918.1| (PREDICTED: LOW QUALITY PROTEIN: general transcription factor IIH subunit 3 [Cucumis melo])

HSP 1 Score: 589.7 bits (1519), Expect = 2.8e-165
Identity = 290/295 (98.31%), Postives = 293/295 (99.32%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLLVVLLDTNPFFWSTS+LPFSKFLSHVLAFLNSILVLNQLNEVVVI
Sbjct: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSSLPFSKFLSHVLAFLNSILVLNQLNEVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
           GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL
Sbjct: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMV IDSC
Sbjct: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVSIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYL+TVFGTDLHSRTFLQLPKSVGVDFRA
Sbjct: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLATVFGTDLHSRTFLQLPKSVGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHK TIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDS+SKLKRKTPE
Sbjct: 241 SCFCHKXTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSLSKLKRKTPE 295

[Close]

BLAST of Cucsa.178810 vs. NCBI nr
Match: gi|645225617|ref|XP_008219657.1| (PREDICTED: general transcription factor IIH subunit 3 [Prunus mume])

HSP 1 Score: 494.2 bits (1271), Expect = 1.6e-136
Identity = 242/295 (82.03%), Postives = 264/295 (89.49%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLL+VLLDTNPFFWS+S+LPFS FLSHVL FLNSIL+LNQLN+VVVI
Sbjct: 1   MASAPSKLYADDVSLLMVLLDTNPFFWSSSSLPFSVFLSHVLTFLNSILLLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
            TGY SC Y+Y+SS+ +N G ++GRMPA C  LL+ LEEFVI DEQ IKE  + G  SSL
Sbjct: 61  ATGYNSCSYIYDSSTSTNQGSDNGRMPARCVNLLQKLEEFVIKDEQLIKEGLREGIASSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQ+VFRSG LHPQPRILCLQGS DGPEQYVAIMNAIFSAQRSMVPIDSC
Sbjct: 121 LSGSLSMALCYIQRVFRSGPLHPQPRILCLQGSSDGPEQYVAIMNAIFSAQRSMVPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           Y+GS NSAFLQQASYITGGVYLKPQQ +GLFQYLSTVF TDLHSR FLQLPKS+GVDFRA
Sbjct: 181 YMGSSNSAFLQQASYITGGVYLKPQQPNGLFQYLSTVFATDLHSRAFLQLPKSLGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHKKTIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+   +++S S  KRKTPE
Sbjct: 241 SCFCHKKTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQSDVNSTSNKKRKTPE 295

[Close]

BLAST of Cucsa.178810 vs. NCBI nr
Match: gi|694371044|ref|XP_009363173.1| (PREDICTED: general transcription factor IIH subunit 3 [Pyrus x bretschneideri])

HSP 1 Score: 488.8 bits (1257), Expect = 6.7e-135
Identity = 242/296 (81.76%), Postives = 261/296 (88.18%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLL+VLLDTNPFFWS+S LPFSKFL HVL FLNSIL+LNQLN+VVVI
Sbjct: 1   MASAPSKLYADDVSLLMVLLDTNPFFWSSSNLPFSKFLPHVLTFLNSILLLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGR-MPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSS 120
            TGY SC Y+Y+SSS SN G + GR MPA C+ LL+ LEEFVI DEQ  KE  + G  SS
Sbjct: 61  ATGYNSCSYIYDSSSDSNQGSDHGRIMPARCSNLLQKLEEFVIKDEQLFKEGSREGISSS 120

Query: 121 LLSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDS 180
           LLSGSLSMALCYIQ+VFRSG LHPQPRILCLQGS DGPEQYVAIMN+IFSAQRSMVPIDS
Sbjct: 121 LLSGSLSMALCYIQRVFRSGPLHPQPRILCLQGSSDGPEQYVAIMNSIFSAQRSMVPIDS 180

Query: 181 CYIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFR 240
           CY+GS NSAFLQQASYITGGVYLKPQQ +GLFQYLSTVF TDLHSR FLQLPKS+GVDFR
Sbjct: 181 CYMGSSNSAFLQQASYITGGVYLKPQQPNGLFQYLSTVFATDLHSRAFLQLPKSLGVDFR 240

Query: 241 ASCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           ASCFCHKKTIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+  ++  S S  KRKTPE
Sbjct: 241 ASCFCHKKTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQLDASSTSNRKRKTPE 296

[Close]

BLAST of Cucsa.178810 vs. NCBI nr
Match: gi|596178523|ref|XP_007223251.1| (hypothetical protein PRUPE_ppa009386mg [Prunus persica])

HSP 1 Score: 487.6 bits (1254), Expect = 1.5e-134
Identity = 241/295 (81.69%), Postives = 263/295 (89.15%), Query Frame = 1


Query: 1   MASAPSKLYADDVSLLVVLLDTNPFFWSTSALPFSKFLSHVLAFLNSILVLNQLNEVVVI 60
           MASAPSKLYADDVSLL+VLLDTNPFFWS+S+LPFS FLSHVL FLNSIL+LNQLN+VVVI
Sbjct: 1   MASAPSKLYADDVSLLMVLLDTNPFFWSSSSLPFSVFLSHVLTFLNSILLLNQLNQVVVI 60

Query: 61  GTGYASCKYLYNSSSYSNHGLEDGRMPALCTRLLKNLEEFVIGDEQSIKEDPKGGTMSSL 120
            TGY SC Y+Y+SS+ +N G ++GRMPA C  LL+ LEEFVI DEQ IKE  + G  SSL
Sbjct: 61  ATGYNSCSYIYDSSTSTNQGSDNGRMPARCVNLLQKLEEFVIEDEQLIKEGLREGIASSL 120

Query: 121 LSGSLSMALCYIQKVFRSGSLHPQPRILCLQGSPDGPEQYVAIMNAIFSAQRSMVPIDSC 180
           LSGSLSMALCYIQ+VFRSG LHPQPRILCLQGS DGPEQYVAIMNAIFSAQRS VPIDSC
Sbjct: 121 LSGSLSMALCYIQRVFRSGPLHPQPRILCLQGSSDGPEQYVAIMNAIFSAQRS-VPIDSC 180

Query: 181 YIGSHNSAFLQQASYITGGVYLKPQQMDGLFQYLSTVFGTDLHSRTFLQLPKSVGVDFRA 240
           Y+GS NSAFLQQASYITGGVYLKPQQ +GLFQYLSTVF TDLHSR FLQLPKS+GVDFRA
Sbjct: 181 YMGSSNSAFLQQASYITGGVYLKPQQPNGLFQYLSTVFATDLHSRAFLQLPKSLGVDFRA 240

Query: 241 SCFCHKKTIDMGYVCSVCLSIFCKHHKKCSTCGSVFGETPVELDSVSKLKRKTPE 296
           SCFCHKKTIDMGY+CSVCLSIFCKHHKKCSTCGSVFG+   +++S S  KRKTPE
Sbjct: 241 SCFCHKKTIDMGYICSVCLSIFCKHHKKCSTCGSVFGQAQSDVNSTSNKKRKTPE 294

The following BLAST results are available for this feature:

BLAST of Cucsa.178810 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TFB4_ARATH	4.6e-120	71.91	RNA polymerase II transcription factor B subunit 4 OS=Arabidopsis thaliana GN=TF...	[more]
TF2H3_DICDI	1.1e-49	41.57	General transcription factor IIH subunit 3 OS=Dictyostelium discoideum GN=gtf2h3...	[more]
TF2H3_HUMAN	1.5e-46	37.58	General transcription factor IIH subunit 3 OS=Homo sapiens GN=GTF2H3 PE=1 SV=2	[more]
TF2H3_BOVIN	4.4e-46	38.03	General transcription factor IIH subunit 3 OS=Bos taurus GN=GTF2H3 PE=2 SV=1	[more]
TF2H3_MOUSE	1.3e-45	38.69	General transcription factor IIH subunit 3 OS=Mus musculus GN=Gtf2h3 PE=1 SV=1	[more]

BLAST of Cucsa.178810 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMM2_CUCSA	4.2e-168	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G072450 PE=4 SV=1	[more]
M5XYU6_PRUPE	1.0e-134	81.69	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009386mg PE=4 SV=1	[more]
A0A061FGT5_THECC	8.2e-132	78.64	Basal transcription factor complex subunit-related isoform 1 OS=Theobroma cacao ...	[more]
E0CR79_VITVI	4.1e-131	79.05	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g03100 PE=4 SV=...	[more]
A0A0D2TQJ3_GOSRA	3.5e-130	77.97	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G245300 PE=4 SV=1	[more]

BLAST of Cucsa.178810 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G18340.1	2.6e-121	71.91	basal transcription factor complex subunit-related	[more]

BLAST of Cucsa.178810 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449470273\|ref\|XP_004152842.1\|	6.0e-168	100.00	PREDICTED: general transcription factor IIH subunit 3 [Cucumis sativus]	[more]
gi\|659082582\|ref\|XP_008441918.1\|	2.8e-165	98.31	PREDICTED: LOW QUALITY PROTEIN: general transcription factor IIH subunit 3 [Cucu...	[more]
gi\|645225617\|ref\|XP_008219657.1\|	1.6e-136	82.03	PREDICTED: general transcription factor IIH subunit 3 [Prunus mume]	[more]
gi\|694371044\|ref\|XP_009363173.1\|	6.7e-135	81.76	PREDICTED: general transcription factor IIH subunit 3 [Pyrus x bretschneideri]	[more]
gi\|596178523\|ref\|XP_007223251.1\|	1.5e-134	81.69	hypothetical protein PRUPE_ppa009386mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR004600	TFIIH_Tfb4/GTF2H3
IPR004600	TFIIH_Tfb4/GTF2H3
IPR004600	TFIIH_Tfb4/GTF2H3
IPR004600	TFIIH_Tfb4/GTF2H3
IPR004600	TFIIH_Tfb4/GTF2H3

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated
GO:0006289	nucleotide-excision repair
GO:0006355	regulation of transcription, DNA-templated
GO:0006289	nucleotide-excision repair
GO:0006355	regulation of transcription, DNA-templated
GO:0006289	nucleotide-excision repair
GO:0006355	regulation of transcription, DNA-templated
GO:0006289	nucleotide-excision repair
GO:0006355	regulation of transcription, DNA-templated
GO:0006289	nucleotide-excision repair

Vocabulary: **Cellular Component**
Term	Definition
GO:0000439	core TFIIH complex
GO:0000439	core TFIIH complex
GO:0000439	core TFIIH complex
GO:0000439	core TFIIH complex
GO:0000439	core TFIIH complex

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006289	nucleotide-excision repair
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation
biological_process	GO:0006366	transcription from RNA polymerase II promoter
cellular_component	GO:0000439	core TFIIH complex
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.178810.1	Cucsa.178810.1	mRNA
Cucsa.178810.2	Cucsa.178810.2	mRNA
Cucsa.178810.3	Cucsa.178810.3	mRNA
Cucsa.178810.4	Cucsa.178810.4	mRNA
Cucsa.178810.5	Cucsa.178810.5	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004600	TFIIH subunit Tfb4/p34	PANTHER	PTHR12831	TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED	coord: 1..210 score: 5.2E
IPR004600	TFIIH subunit Tfb4/p34	PFAM	PF03850	Tfb4	coord: 21..188 score: 2.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.178810	Csa2G072450	Cucumber (Chinese Long) v2	cgycuB274
Cucsa.178810	MELO3C008774	Melon (DHL92) v3.5.1	cgymeB320
Cucsa.178810	CSPI02G06920	Wild cucumber (PI 183967)	cgycpiB284
Cucsa.178810	MELO3C008774.2	Melon (DHL92) v3.6.1	cgymedB315
Cucsa.178810	CsaV3_2G009350	Cucumber (Chinese Long) v3	cgycucB287

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.178810	Wax gourd	cgywgoB366
Cucsa.178810	Cucurbita maxima (Rimu)	cgycmaB0509
Cucsa.178810	Cucurbita maxima (Rimu)	cgycmaB0510
Cucsa.178810	Cucurbita moschata (Rifu)	cgycmoB0509
Cucsa.178810	Cucurbita moschata (Rifu)	cgycmoB0510
Cucsa.178810	Watermelon (Charleston Gray)	cgywcgB319
Cucsa.178810	Watermelon (97103) v1	cgywmB341
Cucsa.178810	Cucurbita pepo (Zucchini)	cgycpeB0491
Cucsa.178810	Cucurbita pepo (Zucchini)	cgycpeB0492
Cucsa.178810	Bottle gourd (USVL1VR-Ls)	cgylsiB300
Cucsa.178810	Silver-seed gourd	carcgyB0005
Cucsa.178810	Silver-seed gourd	carcgyB0204
Cucsa.178810	Watermelon (97103) v2	cgywmbB323