Cucsa.146830 (gene) Cucumber (Gy14) v1

NameCucsa.146830
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRNA-binding family protein
Locationscaffold01100 : 170478 .. 173711 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCGTTGAAGGCATCCGTGATTGAGTGAGAATCGATAAGAACGCTTCGTCTACTTTAGCTTTGATTTGGGTTTTGCCTTCGCTGATCTGTACGGTAAGTTCCCTTTCTGTGTTTCGTTTTATTTTCGTTTGAGATGAAATGTTTGATCTGGATATGCATTTGATTCTGCTTTCGAACAGGCAGAATTAGGTTGGGGTTATTGTTTTATTGTTTTTTCTGTGATGAGCATGTGCATCCCTTTTTGTTGTTTGAGATGATATGTTCGGGGTTCTGATCGACATGCGCGTTTGATTCCGCTCTCTGTAATGTTTATGGAAAGAATTATATTCTGGTTATTGAGCGGGTTTTGTTTACTCTATGATTCGCTAGTTATGGGATGCAAATGGAGTAGTTTCTGTAATTTACTTGAAAATTTGATTGTTAAATTAGAACCACTTCGGAGATGTTGAAGTCAACCATATTTCTTTTTATTGGAGTCATGGATCTTAAATTGCTAATTTGGTATCATTTGGAGATCGTCCGAGTTTAATCAATTTGGTTTATAATTCTAATTTAGATCGATAGAAGTATAGAACACTCGTAAATATTCCTTGCAGTATGAATTATCTATGAAATTTTTATTAACGCATAAGGACTATTTTTTGAGGTTTTATTAAATCAAACTTAATTCGAATTTTTTTTTAAAACCATAGGACTAAACTAGCTTTCCCCAAACCATAGGAACTTAACTTGCAAGTTAATCTTTTCTTTACTTACGACAGCTCCTTGCGCTGTTCTGTTCAAGACGATATTCATACTTAATAGTACTCTTAACATTATGGTCTTCATCGACGCTTGTGCTTCTCGTTTTAAATCTCTGTTGAATTAAGTGATTTCTTGTTTGGTTCCAGATGTCGTACTCCCGAAGGTCTAGATACTCCCGCTCCCCATCATTCAAACGATCTGTCTCAAGGTCTCTCTCGAGGTCAAGAAGCCGTTCAAGGTACTTCCTAACCAGAAACCACTTACTTGCTTGATTTTCCCACCTTCTCTAAATGATACGATGATGCATTTATTCTTATTGTATGGATTCTTAATTCGTTTTTCAGGAGCCCATCAAGTGATGTTGAAAATCCTGGGAACAATTTGTATGTCACTGGGTTGTCACCTAGAATAACTAGAAGAGAACTCGAGAAGCATTTTTCTGCGGAGGGGACAGTATGTTCCTGATCTCTCATTTTTTGTTCATTCCTTCTTTCTTTTTTTCCTGGCATGCTATTCTTAGTGGCCGTGTATGTATTTTGTCTTTTGTGTTGGTGATTTAAGTTGTGGACTATCCCCCGTTCATGAATGGATGTCTTTAACTCCAGGTTCTAGATGTTCATCTTGTGGCCGATCCGTTGACTAGAGAATCTCGTGGATTTGGATTCATTACCATGTCTAGTAATGATGAGGCGGAAAATTGCATCAAATATCTGAATCGATCTGTACTTGAAGGTCGTATAATTACTGTGGAAAAGGTAAAAAGTGAACTAATTATCTCTTCATAGACTTGGGATGTTTCCTATTGTTACTGTTGTCTTGTTCTTTAATCTGAAATGGTGTACCTGGATGATTTGATGTTAGAGATTTGAGTTTTTTTTGCCAAGTGTTTTTTTGGCAGCAGGTTAGCTGTGTGTAGCAGCTCTTCATCAAACTCAAACTACAACTAAACAATCAAAACAAACACATGAAGCCTCAGAACATTGGGGTTGTATGCCCTGCATTTCTCTCTATTTCTGCCAATTGCATATGTGTTGAGTTATTAAAGTGTTTTAGCTTCTTTTTTCTTTTGTGAATCTCGACACTGTGACTTTGCATCCGTGCTATGGTATTTTTCTTGGATGGAAGCTTGAGTAGCTGGTCTCACTGTTTATCTTTAATTGTTTGACAGGCTCGGAGACGGAGAGGCAGAACTCCAACACCTGGTAGATATCTTGGACTTAGAACAGTTCATGGTGAGATTAAAATCCAATGTTTGGCATATTGCTGTGAAATATCTTTGATATTTCTGTCTAATCCTCATCATTCTGACAGGGAGGCGTAGGTCGCGTAGCTACTCATCTCGGCGTTCTCCCAGCTACTCTCCATATAGAAGAAGCTCTAGCCGCTCTACGCATTGTTCATCTGACCGGAGCAGAAGTAGATCCCGTTCCATTGACCAACATCGTAGAGCATATGGTTCCCCATACCATCGTAGATCGTACTCTTATAGCCGGCGAAGATATGATTCTCCAGACTATAGGCGACGTTATGATTCTCCAGACTACTATAGGCATCATAAGTCATATTCTCGATCTCGCTCACCATATAGGAGGTGGAGCCGTGACAGGTCATACTCACCTTGTGATCACTATTCCCCTGATCCTTACTACAGAAGGCACAGGTACAGGTCAGTTTCTCGGAGCCTTTCTCCGAAGTCAAGGAACAGCAGACATTACTCCCGAAGTTACTCTCCTGAGCCTAGGAGAAGAACGTTGAGAAACCACTATCGCAGCCCCTCACAGGAATTGAGTAGTTATTCACGTAGTATTTCCCCTGAACGAAGCAGATCAGGAAGCAGTATCTATTCATCCAGTATTTCTCCTGAACGAGGCAGGAGCTCAAGGAGTCGCTCTTGTAGCCATACACCAAGACGACACGAGAGCACACGTAAGAGAGGGTATTCTAGTAGGAGTCGATCGAGAAGCCAAAGTGTAACTTCAAGATCTGTTAGTCCGGCTTGATTAACTGATCTACTTCCCCTCTGCTACATTCTCATGATGATGTACATTTGGAGCTTTTAATACAAATCAATCAGCTGCAGATGTAAACAACTATTCCCCTTGTCTTACGGATGCCTTAGCGTTTGAGATGAAATAGTGGGTTGTGGAATAATTTATTTCTGGTTTGGTTTAATGGCGTACTAGGTGAAACGGAGAAATGTCGACAGGGAAAATGTTGTACGCTCTCAACTAATGTTCGTTCTTAATTCTATTGGGTTAGTTTGTTAACGAGGGGTGTGAAATGTTTTGGTTTAATTTGCCATTTGGATTTCCTACACTATTTGGGTTGGATGTTATGCCTTATCTGAACATGAAAAGATGCCGTACATTTGTGGTTTACCCTAATTGGATTATGCTGTTCTTTAACCTAATATAAACCTCATCTCTACTTTGGGAGTGGTATGAATTTTTTCGTGTATTACTATAAAATTTCTCCTATTCATCCAATAA

mRNA sequence

TGTCGTTGAAGGCATCCGTGATTGAGTGAGAATCGATAAGAACGCTTCGTCTACTTTAGCTTTGATTTGGGTTTTGCCTTCGCTGATCTGTACGATGTCGTACTCCCGAAGGTCTAGATACTCCCGCTCCCCATCATTCAAACGATCTGTCTCAAGGTCTCTCTCGAGGTCAAGAAGCCGTTCAAGGAGCCCATCAAGTGATGTTGAAAATCCTGGGAACAATTTGTATGTCACTGGGTTGTCACCTAGAATAACTAGAAGAGAACTCGAGAAGCATTTTTCTGCGGAGGGGACAGTTCTAGATGTTCATCTTGTGGCCGATCCGTTGACTAGAGAATCTCGTGGATTTGGATTCATTACCATGTCTAGTAATGATGAGGCGGAAAATTGCATCAAATATCTGAATCGATCTGTACTTGAAGGTCGTATAATTACTGTGGAAAAGGCTCGGAGACGGAGAGGCAGAACTCCAACACCTGGGAGGCGTAGGTCGCGTAGCTACTCATCTCGGCGTTCTCCCAGCTACTCTCCATATAGAAGAAGCTCTAGCCGCTCTACGCATTGTTCATCTGACCGGAGCAGAAGTAGATCCCGTTCCATTGACCAACATCGTAGAGCATATGGTTCCCCATACCATCGTAGATCGTACTCTTATAGCCGGCGAAGATATGATTCTCCAGACTATAGGCGACGTTATGATTCTCCAGACTACTATAGGCATCATAAGTCATATTCTCGATCTCGCTCACCATATAGGAGGTGGAGCCGTGACAGGTCATACTCACCTTGTGATCACTATTCCCCTGATCCTTACTACAGAAGGCACAGGTACAGGTCAGTTTCTCGGAGCCTTTCTCCGAAGTCAAGGAACAGCAGACATTACTCCCGAAGTTACTCTCCTGAGCCTAGGAGAAGAACGTTGAGAAACCACTATCGCAGCCCCTCACAGGAATTGAGTAGTTATTCACGTAGTATTTCCCCTGAACGAAGCAGATCAGGAAGCAGTATCTATTCATCCAGTATTTCTCCTGAACGAGGCAGGAGCTCAAGGAGTCGCTCTTGTAGCCATACACCAAGACGACACGAGAGCACACGTAAGAGAGGGTATTCTAGTAGGAGTCGATCGAGAAGCCAAAGTGTAACTTCAAGATCTGTTAGTCCGGCTTGATTAACTGATCTACTTCCCCTCTGCTACATTCTCATGATGATGTACATTTGGAGCTTTTAATACAAATCAATCAGCTGCAGATGTAAACAACTATTCCCCTTGTCTTACGGATGCCTTAGCGTTTGAGATGAAATAGTGGGTTGTGGAATAATTTATTTCTGGTTTGGTTTAATGGCGTACTAGGTGAAACGGAGAAATGTCGACAGGGAAAATGTTGTACGCTCTCAACTAATGTTCGTTCTTAATTCTATTGGGTTAGTTTGTTAACGAGGGGTGTGAAATGTTTTGGTTTAATTTGCCATTTGGATTTCCTACACTATTTGGGTTGGATGTTATGCCTTATCTGAACATGAAAAGATGCCGTACATTTGTGGTTTACCCTAATTGGATTATGCTGTTCTTTAACCTAATATAAACCTCATCTCTACTTTGGGAGTGGTATGAATTTTTTCGTGTATTACTATAAAATTTCTCCTATTCATCCAATAA

Coding sequence (CDS)

ATGTCGTACTCCCGAAGGTCTAGATACTCCCGCTCCCCATCATTCAAACGATCTGTCTCAAGGTCTCTCTCGAGGTCAAGAAGCCGTTCAAGGAGCCCATCAAGTGATGTTGAAAATCCTGGGAACAATTTGTATGTCACTGGGTTGTCACCTAGAATAACTAGAAGAGAACTCGAGAAGCATTTTTCTGCGGAGGGGACAGTTCTAGATGTTCATCTTGTGGCCGATCCGTTGACTAGAGAATCTCGTGGATTTGGATTCATTACCATGTCTAGTAATGATGAGGCGGAAAATTGCATCAAATATCTGAATCGATCTGTACTTGAAGGTCGTATAATTACTGTGGAAAAGGCTCGGAGACGGAGAGGCAGAACTCCAACACCTGGGAGGCGTAGGTCGCGTAGCTACTCATCTCGGCGTTCTCCCAGCTACTCTCCATATAGAAGAAGCTCTAGCCGCTCTACGCATTGTTCATCTGACCGGAGCAGAAGTAGATCCCGTTCCATTGACCAACATCGTAGAGCATATGGTTCCCCATACCATCGTAGATCGTACTCTTATAGCCGGCGAAGATATGATTCTCCAGACTATAGGCGACGTTATGATTCTCCAGACTACTATAGGCATCATAAGTCATATTCTCGATCTCGCTCACCATATAGGAGGTGGAGCCGTGACAGGTCATACTCACCTTGTGATCACTATTCCCCTGATCCTTACTACAGAAGGCACAGGTACAGGTCAGTTTCTCGGAGCCTTTCTCCGAAGTCAAGGAACAGCAGACATTACTCCCGAAGTTACTCTCCTGAGCCTAGGAGAAGAACGTTGAGAAACCACTATCGCAGCCCCTCACAGGAATTGAGTAGTTATTCACGTAGTATTTCCCCTGAACGAAGCAGATCAGGAAGCAGTATCTATTCATCCAGTATTTCTCCTGAACGAGGCAGGAGCTCAAGGAGTCGCTCTTGTAGCCATACACCAAGACGACACGAGAGCACACGTAAGAGAGGGTATTCTAGTAGGAGTCGATCGAGAAGCCAAAGTGTAACTTCAAGATCTGTTAGTCCGGCTTGA

Protein sequence

MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARRRRGRTPTPGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSIDQHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVTSRSVSPA*
BLAST of Cucsa.146830 vs. Swiss-Prot
Match: SR45A_ARATH (Serine/arginine-rich splicing factor SR45a OS=Arabidopsis thaliana GN=SR45A PE=1 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 3.9e-76
Identity = 204/381 (53.54%), Postives = 232/381 (60.89%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS-FKRSVSRSLSRSRSRS--RSPSSDVENPGNNLYVTGLSPRITRRE 60
           MSYSRRSRYS S S + +   RS+SRS SRS  RS SSD ENPGN+LYVTGLS R+T R+
Sbjct: 31  MSYSRRSRYSPSLSPYDKRRGRSVSRSLSRSPTRSVSSDAENPGNSLYVTGLSHRVTERD 90

Query: 61  LEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEK 120
           LE HF+ EG V DVHLV DP TRESRGFGFI+M S  +A  CI+ L+ SVL+GR+ITVEK
Sbjct: 91  LEDHFAKEGKVTDVHLVLDPWTRESRGFGFISMKSVGDANRCIRSLDHSVLQGRVITVEK 150

Query: 121 ARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSR 180
           ARRRRGRTPTPG+YLGLRT  GR +S SYS RRS S      S SRS   SSDR RS S 
Sbjct: 151 ARRRRGRTPTPGKYLGLRTARGRHKSPSYSPRRSVSC-----SRSRSRSYSSDRGRSYSP 210

Query: 181 SIDQHRRAYG-SPYHRRSYSYSRRRYDSPD--YRRRYDSPDYYRHHKSYSRSRSP-YRRW 240
           S  +  R+   SP++RR   YS  R  SPD  Y RR D            RS SP YRR 
Sbjct: 211 SYGRRGRSSSYSPFYRRRRFYSPSRSPSPDDRYNRRRD------------RSYSPYYRRR 270

Query: 241 SRDRSYS-PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRS 300
            R RSYS  C      PYY R RYRS SRS SP+ R +R  S S     R R+   HY+ 
Sbjct: 271 DRSRSYSRNCRARDRSPYYMR-RYRSRSRSYSPRYR-ARDRSCSPYYRGRDRSYSPHYQG 330

Query: 301 PSQELSSYSRSISPERSRSGS-----SIYSSSISPERGR-SSRSRSCSHTPRRHESTRKR 360
             +  S  SR     RS SGS        S SISP +GR  SRS+S     RRH+     
Sbjct: 331 RDRSYSPESRYYRRHRSVSGSVSPGGRSMSRSISPRKGRKESRSKS-----RRHDR---- 382

Query: 361 GYSSRSRSRS-QSVTSRSVSP 367
             SS   SRS +S TSRSVSP
Sbjct: 391 -QSSMCHSRSARSSTSRSVSP 382

BLAST of Cucsa.146830 vs. Swiss-Prot
Match: YDC1_SCHPO (Uncharacterized RNA-binding protein C25G10.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC25G10.01 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.1e-22
Identity = 74/227 (32.60%), Postives = 114/227 (50.22%), Query Frame = 1

Query: 20  SRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLT 79
           + S +  +   +S     EN GN+L+V+G++ R+   EL++ FS  GTV  V ++ +P+T
Sbjct: 79  NESTALDKKEPQSAPEGSENLGNDLFVSGIASRMQEDELQQIFSKFGTVTHVRIMREPVT 138

Query: 80  RESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARRRRGRTPTPGRYLGLRTVHG 139
           + SRGFGF++ S+ +EA + I  LN     GR++ V+KA+R R  +PTPG+Y+G      
Sbjct: 139 KASRGFGFLSFSTVEEATSAIDNLNSQEFYGRVLNVQKAKRSRPHSPTPGKYMG---YDR 198

Query: 140 RRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSIDQHRRAYGSPYHRRSYSYSR 199
           RR SR + S        YRR++ R      DR  +R               +R SY  SR
Sbjct: 199 RRNSRDFPSNNKD--GGYRRNNYR------DRDSNR---------------YRNSYRPSR 258

Query: 200 RRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYS 247
                    +R  SP  YR  +    SR    R    RS++  +H+S
Sbjct: 259 --------PQREHSPGNYRKERYNVDSRPRRERHFHGRSFAHAEHHS 271

BLAST of Cucsa.146830 vs. Swiss-Prot
Match: SC35_ARATH (Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana GN=SC35 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-21
Identity = 110/320 (34.38%), Postives = 142/320 (44.38%), Query Frame = 1

Query: 43  NLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKY 102
           +L V  ++ R T  +L   F+  G V+DV +  D  T +SRGF F+     DEA   ++ 
Sbjct: 17  SLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKDEAHKAVER 76

Query: 103 LNRSVLEGRIITVE---------------------KARRRRGRTPTPGRYLGLRTVHGRR 162
           L+  V++GR ITV+                     K+RR R R+P        R     R
Sbjct: 77  LDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRSP--------RRSRSPR 136

Query: 163 RSRSYSSRRSPSYSPYRRSSSRSTHCSSD-RSRSRSRSIDQHRRAYGSPY-HRRSYSYSR 222
           RSRS   RRSP  S   R  SR  +   D R RSRSRS D+  R       HRR    +R
Sbjct: 137 RSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRSYDRRERHEEKDRDHRRR---TR 196

Query: 223 RRYDSPDYRRRYDSPDYYRHHKSYSR--SRSPYRRWSRDRS---YSPCDHYSPDPYYRRH 282
            R  SPD +RR     Y    +S+SR  S SP RR  R  S    SP    SPD      
Sbjct: 197 SRSASPDEKRRVRG-RYDNESRSHSRSLSASPARRSPRSSSPQKTSPAREVSPDK----- 256

Query: 283 RYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSYSRSISPERSRSGSS 335
             RS  RS SP+        RS SP           RSP+ + +S S+ +SPER  +  S
Sbjct: 257 --RSNERSPSPR--------RSLSP-----------RSPALQKASPSKEMSPERRSNERS 298


HSP 2 Score: 61.6 bits (148), Expect = 2.1e-08
Identity = 62/185 (33.51%), Postives = 81/185 (43.78%), Query Frame = 1

Query: 190 YHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSP----CDHY 249
           Y   +   S+ R   P  + R       R  +S  RSRSP RR S  RS SP     D Y
Sbjct: 94  YGPNAEKISKGRVVEPPPKSRRSRSRSPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDY 153

Query: 250 SPDPYYRRHRYRSVSRSLSPKSRNSRH----YSRSYSPEPRRRTLRNHYRSPSQELSSYS 309
               Y +R R RS  R    + ++  H     SRS SP+ +RR +R  Y + S+   S+S
Sbjct: 154 REKDYRKRSRSRSYDRRERHEEKDRDHRRRTRSRSASPDEKRR-VRGRYDNESR---SHS 213

Query: 310 RSIS---PERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQS 364
           RS+S     RS   SS   +S + E     RS   S +PRR  S R       S S+  S
Sbjct: 214 RSLSASPARRSPRSSSPQKTSPAREVSPDKRSNERSPSPRRSLSPRSPALQKASPSKEMS 273

BLAST of Cucsa.146830 vs. Swiss-Prot
Match: SRSF2_PIG (Serine/arginine-rich splicing factor 2 OS=Sus scrofa GN=SRSF2 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.7e-18
Identity = 81/212 (38.21%), Postives = 103/212 (48.58%), Query Frame = 1

Query: 33  PSSDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSS 92
           P  DVE    +L V  L+ R +   L + F   G V DV++  D  T+ESRGF F+    
Sbjct: 6   PPPDVEGM-TSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHD 65

Query: 93  NDEAENCIKYLNRSVLEGRIITVEKARRRR-------GRTPTPGRY----LGLRTVHGRR 152
             +AE+ +  ++ +VL+GR + V+ AR  R        R P P RY     G R+   RR
Sbjct: 66  KRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRR 125

Query: 153 ----RSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSIDQHRRAYGSPYHRRSYSY 212
               RSRS S  RS S S Y RS SRS   S  RS S+SRS  + +    S    RS S 
Sbjct: 126 RRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSR 185

Query: 213 SRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSP 230
           SR R  SP        P   R  KS SRS+SP
Sbjct: 186 SRSRSRSP-------PPASKRESKSRSRSKSP 209


HSP 2 Score: 82.8 bits (203), Expect = 8.6e-15
Identity = 84/240 (35.00%), Postives = 107/240 (44.58%), Query Frame = 1

Query: 33  PSSDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSS 92
           P  DVE    +L V  L+ R +   L + F   G V DV++  D  T+ESRGF F+    
Sbjct: 6   PPPDVEGM-TSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHD 65

Query: 93  NDEAENCIKYLNRSVLEGRIITVEKARRRRG-------RTPTPGRYLGLRTVHGRRRSRS 152
             +AE+ +  ++ +VL+GR + V+ AR  R        R P P RY G    +GRR    
Sbjct: 66  KRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGG--GGYGRR---- 125

Query: 153 YSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSIDQHRRAYGSPYHRRSYSYSRRRYDSP 212
                  S SP RR  SRS   S  RSRSRSR             + RS S SR R  S 
Sbjct: 126 -------SRSPRRRRRSRSRSRSRSRSRSRSR-------------YSRSKSRSRTRSRSR 185

Query: 213 DYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYSPDPYYRRHRYRSVSRSLSP 266
              +   +        S SRSRS  R  SR R        SP P  +R   +S SRS SP
Sbjct: 186 STSKSRSARRSKSKSSSVSRSRSRSRSRSRSR--------SPPPASKRES-KSRSRSKSP 209


HSP 3 Score: 48.5 bits (114), Expect = 1.8e-04
Identity = 57/150 (38.00%), Postives = 65/150 (43.33%), Query Frame = 1

Query: 190 YHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYSPDP 249
           Y R   S+  RR   P   RRY    Y R      RSRSP RR                 
Sbjct: 92  YGRPPDSHHSRRGPPP---RRYGGGGYGR------RSRSPRRR----------------- 151

Query: 250 YYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSYSRSISPERS 309
             RR R RS SRS   +SR+   YSRS S    R   R+  +S S   S  S+S S  RS
Sbjct: 152 --RRSRSRSRSRS---RSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSK-SKSSSVSRS 209

Query: 310 RSGSSIYSSSISPERG--RSSRSRSCSHTP 338
           RS S   S S SP     R S+SRS S +P
Sbjct: 212 RSRSRSRSRSRSPPPASKRESKSRSRSKSP 209


HSP 4 Score: 41.6 bits (96), Expect = 2.2e-02
Identity = 45/102 (44.12%), Postives = 51/102 (50.00%), Query Frame = 1

Query: 272 HYSRSYSPEPRRRT---LRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSS 331
           H+SR   P PRR          RSP +   S SRS S  RSRS S  YS S S      S
Sbjct: 99  HHSRR-GPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSR-YSRSKS-----RS 158

Query: 332 RSRSCSHTPRRHESTR----KRGYSSRSRSRSQSVTSRSVSP 367
           R+RS S +  +  S R    K    SRSRSRS+S  SRS SP
Sbjct: 159 RTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRS-RSRSRSP 192


HSP 5 Score: 35.8 bits (81), Expect = 1.2e+00
Identity = 21/32 (65.62%), Postives = 23/32 (71.88%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSP 34
           S SR +R S+S S   S SRS SRSRSRSRSP
Sbjct: 161 SKSRSARRSKSKSSSVSRSRSRSRSRSRSRSP 192


HSP 6 Score: 34.3 bits (77), Expect = 3.5e+00
Identity = 21/38 (55.26%), Postives = 24/38 (63.16%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVEN 40
           S S R   S+S S  RS SRS SRSRSRS  P+S  E+
Sbjct: 163 SRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPASKRES 200


HSP 7 Score: 32.3 bits (72), Expect = 1.3e+01
Identity = 21/36 (58.33%), Postives = 24/36 (66.67%), Query Frame = 1

Query: 6   RSRYSRSPSFKRSVSRSLSRSRS----RSRSPSSDV 38
           RSRYSRS S  R+ SRS S S+S    RS+S SS V
Sbjct: 141 RSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSV 176


HSP 8 Score: 32.0 bits (71), Expect = 1.7e+01
Identity = 20/39 (51.28%), Postives = 23/39 (58.97%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENP 41
           S SR +  SRS    +S S S+SRSRSRSRS S     P
Sbjct: 155 SRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPP 193


HSP 9 Score: 32.0 bits (71), Expect = 1.7e+01
Identity = 20/30 (66.67%), Postives = 21/30 (70.00%), Query Frame = 1

Query: 3   YSRRSRYSRSPSFKRSVSRSLSRSRSRSRS 33
           Y RRSR  R    +RS SRS SRSRSRSRS
Sbjct: 115 YGRRSRSPRRR--RRSRSRSRSRSRSRSRS 142


HSP 10 Score: 30.0 bits (66), Expect = 6.6e+01
Identity = 22/49 (44.90%), Postives = 25/49 (51.02%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSL---------SRSRSRSRSPSSDVENPG 42
           S S+ S  SRS S  RS SRS          S+SRSRS+SP    E  G
Sbjct: 169 SKSKSSSVSRSRSRSRSRSRSRSPPPASKRESKSRSRSKSPPKSPEEEG 217


HSP 11 Score: 29.6 bits (65), Expect = 8.6e+01
Identity = 21/38 (55.26%), Postives = 23/38 (60.53%), Query Frame = 1

Query: 5   RRSRYSRSPSFKRS-------VSRSLSRSRSRSRSPSS 36
           RR R SRS S  RS        SRS SRSR+RSRS S+
Sbjct: 123 RRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRST 160


HSP 12 Score: 29.3 bits (64), Expect = 1.1e+02
Identity = 23/43 (53.49%), Postives = 23/43 (53.49%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKR----------SVSRSLSRSRSRSRSPS 35
           S SR    SRS S  R          SVSRS SRSRSRSRS S
Sbjct: 149 SRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRS 191

BLAST of Cucsa.146830 vs. Swiss-Prot
Match: SRSF2_HUMAN (Serine/arginine-rich splicing factor 2 OS=Homo sapiens GN=SRSF2 PE=1 SV=4)

HSP 1 Score: 94.7 bits (234), Expect = 2.2e-18
Identity = 81/212 (38.21%), Postives = 103/212 (48.58%), Query Frame = 1

Query: 33  PSSDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSS 92
           P  DVE    +L V  L+ R +   L + F   G V DV++  D  T+ESRGF F+    
Sbjct: 6   PPPDVEGM-TSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHD 65

Query: 93  NDEAENCIKYLNRSVLEGRIITVEKARRRR-------GRTPTPGRY----LGLRTVHGRR 152
             +AE+ +  ++ +VL+GR + V+ AR  R        R P P RY     G R+   RR
Sbjct: 66  KRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRR 125

Query: 153 ----RSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSIDQHRRAYGSPYHRRSYSY 212
               RSRS S  RS S S Y RS SRS   S  RS S+SRS  + +    S    RS S 
Sbjct: 126 RRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSR 185

Query: 213 SRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSP 230
           SR R  SP        P   R  KS SRS+SP
Sbjct: 186 SRSRSRSP-------PPVSKRESKSRSRSKSP 209


HSP 2 Score: 83.2 bits (204), Expect = 6.6e-15
Identity = 84/240 (35.00%), Postives = 107/240 (44.58%), Query Frame = 1

Query: 33  PSSDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSS 92
           P  DVE    +L V  L+ R +   L + F   G V DV++  D  T+ESRGF F+    
Sbjct: 6   PPPDVEGM-TSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHD 65

Query: 93  NDEAENCIKYLNRSVLEGRIITVEKARRRRG-------RTPTPGRYLGLRTVHGRRRSRS 152
             +AE+ +  ++ +VL+GR + V+ AR  R        R P P RY G    +GRR    
Sbjct: 66  KRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGG--GGYGRR---- 125

Query: 153 YSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSIDQHRRAYGSPYHRRSYSYSRRRYDSP 212
                  S SP RR  SRS   S  RSRSRSR             + RS S SR R  S 
Sbjct: 126 -------SRSPRRRRRSRSRSRSRSRSRSRSR-------------YSRSKSRSRTRSRSR 185

Query: 213 DYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYSPDPYYRRHRYRSVSRSLSP 266
              +   +        S SRSRS  R  SR R        SP P  +R   +S SRS SP
Sbjct: 186 STSKSRSARRSKSKSSSVSRSRSRSRSRSRSR--------SPPPVSKRES-KSRSRSKSP 209


HSP 3 Score: 48.1 bits (113), Expect = 2.3e-04
Identity = 57/150 (38.00%), Postives = 65/150 (43.33%), Query Frame = 1

Query: 190 YHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYSPDP 249
           Y R   S+  RR   P   RRY    Y R      RSRSP RR                 
Sbjct: 92  YGRPPDSHHSRRGPPP---RRYGGGGYGR------RSRSPRRR----------------- 151

Query: 250 YYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSYSRSISPERS 309
             RR R RS SRS   +SR+   YSRS S    R   R+  +S S   S  S+S S  RS
Sbjct: 152 --RRSRSRSRSRS---RSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSK-SKSSSVSRS 209

Query: 310 RSGSSIYSSSISPE--RGRSSRSRSCSHTP 338
           RS S   S S SP     R S+SRS S +P
Sbjct: 212 RSRSRSRSRSRSPPPVSKRESKSRSRSKSP 209


HSP 4 Score: 41.6 bits (96), Expect = 2.2e-02
Identity = 45/102 (44.12%), Postives = 51/102 (50.00%), Query Frame = 1

Query: 272 HYSRSYSPEPRRRT---LRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSS 331
           H+SR   P PRR          RSP +   S SRS S  RSRS S  YS S S      S
Sbjct: 99  HHSRR-GPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSR-YSRSKS-----RS 158

Query: 332 RSRSCSHTPRRHESTR----KRGYSSRSRSRSQSVTSRSVSP 367
           R+RS S +  +  S R    K    SRSRSRS+S  SRS SP
Sbjct: 159 RTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRS-RSRSRSP 192


HSP 5 Score: 35.8 bits (81), Expect = 1.2e+00
Identity = 21/32 (65.62%), Postives = 23/32 (71.88%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSP 34
           S SR +R S+S S   S SRS SRSRSRSRSP
Sbjct: 161 SKSRSARRSKSKSSSVSRSRSRSRSRSRSRSP 192


HSP 6 Score: 32.3 bits (72), Expect = 1.3e+01
Identity = 21/36 (58.33%), Postives = 24/36 (66.67%), Query Frame = 1

Query: 6   RSRYSRSPSFKRSVSRSLSRSRS----RSRSPSSDV 38
           RSRYSRS S  R+ SRS S S+S    RS+S SS V
Sbjct: 141 RSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSV 176


HSP 7 Score: 32.0 bits (71), Expect = 1.7e+01
Identity = 20/39 (51.28%), Postives = 23/39 (58.97%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENP 41
           S SR +  SRS    +S S S+SRSRSRSRS S     P
Sbjct: 155 SRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPP 193


HSP 8 Score: 32.0 bits (71), Expect = 1.7e+01
Identity = 20/30 (66.67%), Postives = 21/30 (70.00%), Query Frame = 1

Query: 3   YSRRSRYSRSPSFKRSVSRSLSRSRSRSRS 33
           Y RRSR  R    +RS SRS SRSRSRSRS
Sbjct: 115 YGRRSRSPRRR--RRSRSRSRSRSRSRSRS 142


HSP 9 Score: 30.8 bits (68), Expect = 3.9e+01
Identity = 21/43 (48.84%), Postives = 24/43 (55.81%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRS---VSRSLSRSRSRSRSPSSDVENPG 42
           S SR    SRS S  RS   VS+  S+SRSRS+SP    E  G
Sbjct: 175 SVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEG 217


HSP 10 Score: 29.6 bits (65), Expect = 8.6e+01
Identity = 21/38 (55.26%), Postives = 23/38 (60.53%), Query Frame = 1

Query: 5   RRSRYSRSPSFKRS-------VSRSLSRSRSRSRSPSS 36
           RR R SRS S  RS        SRS SRSR+RSRS S+
Sbjct: 123 RRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRST 160


HSP 11 Score: 29.3 bits (64), Expect = 1.1e+02
Identity = 23/43 (53.49%), Postives = 23/43 (53.49%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKR----------SVSRSLSRSRSRSRSPS 35
           S SR    SRS S  R          SVSRS SRSRSRSRS S
Sbjct: 149 SRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRS 191

BLAST of Cucsa.146830 vs. TrEMBL
Match: A0A0A0LXJ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528490 PE=4 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 5.0e-211
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK 60
           MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK
Sbjct: 1   MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK 60

Query: 61  HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR 120
           HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR
Sbjct: 61  HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR 120

Query: 121 RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID 180
           RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID
Sbjct: 121 RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID 180

Query: 181 QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS 240
           QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS
Sbjct: 181 QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS 240

Query: 241 PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSY 300
           PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSY
Sbjct: 241 PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSY 300

Query: 301 SRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVT 360
           SRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVT
Sbjct: 301 SRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVT 360

Query: 361 SRSVSPA 368
           SRSVSPA
Sbjct: 361 SRSVSPA 367

BLAST of Cucsa.146830 vs. TrEMBL
Match: B9S368_RICCO (FUS-interacting serine-arginine-rich protein 1, putative OS=Ricinus communis GN=RCOM_0730610 PE=4 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.0e-107
Identity = 241/397 (60.71%), Postives = 273/397 (68.77%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRR 60
           MSYS+RSRYS SPS    + RSVSRS+SRSRSRSRSPSSDVENPGNNLYVTGLSPRIT+R
Sbjct: 2   MSYSKRSRYSPSPSPYRRYSRSVSRSMSRSRSRSRSPSSDVENPGNNLYVTGLSPRITKR 61

Query: 61  ELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVE 120
           +LEKHF++EG V+DVHLV DP TRESRGFGF+TMS+  EA+ CIKYLNRSVLEGR+ITVE
Sbjct: 62  DLEKHFASEGKVIDVHLVVDPWTRESRGFGFVTMSTLVEADRCIKYLNRSVLEGRVITVE 121

Query: 121 KARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRS 180
           KA+RRRGRTPTPGRYLGLRT+  RR + S+S  RSPSYS YR S SRS   SS+RSRSRS
Sbjct: 122 KAKRRRGRTPTPGRYLGLRTIRVRRLTPSHSPHRSPSYSRYRGSLSRSPRYSSERSRSRS 181

Query: 181 RSIDQHRRA--------YGSPYHRRSYS--YSRRRYDSPDYR-RRYDSPDYYRHHKSYSR 240
            S    RR+        YG     RSYS  Y RRR  SP YR RR  SP YY  H+SYSR
Sbjct: 182 YSPHYRRRSRSRSFSPSYGRRSRSRSYSPYYRRRRSYSPYYRSRRSYSPYYYSRHRSYSR 241

Query: 241 SRSPYRR---WSRDRSYSPCDHYSPDP---YYRRHRYRSVSRSLSPKSRNS-RHYSRSYS 300
           SRSPY R      DRSYSP       P   YYRRHR+RSVS S   ++R S R YSRS S
Sbjct: 242 SRSPYSRSPVSRHDRSYSPYGSRYDSPGERYYRRHRHRSVSSSPGRRTRRSARSYSRSIS 301

Query: 301 PEPRRRTLRNHYRS----PSQELSSYSRSISPERSRSGSSIYSSSISPER---GRSSRSR 360
           P PRR + R++  S    P     SYSRS SP   +S    YS SISP R    R SRSR
Sbjct: 302 PSPRRSSRRSYSSSRSPPPRSSRKSYSRSPSPRMRKSLKRSYSRSISPIRRKSSRRSRSR 361

Query: 361 SCSHTPRRHES-TRKRGYSSRSRSRSQSVTSRSVSPA 368
           S S      ++ +R R  S+ SRS S+S T RS SP+
Sbjct: 362 SASSVHDSQDTYSRSRNLSASSRSVSRSPTPRSASPS 398

BLAST of Cucsa.146830 vs. TrEMBL
Match: A0A0R0IW62_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G334100 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 9.0e-104
Identity = 237/385 (61.56%), Postives = 264/385 (68.57%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSR--SRSRSRSPSSDVENPGNNLYVTGLSPRIT 60
           MSYSRRSRYSRSPS    + RSVSRSLSR  SRSRSRS S D ENPGNNLYVTGLSPRIT
Sbjct: 1   MSYSRRSRYSRSPSPYKRYSRSVSRSLSRTSSRSRSRSLSRDAENPGNNLYVTGLSPRIT 60

Query: 61  RRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIIT 120
           +RELEKHFSAEG V+DVHLV DP TRESRGFGF+TM + +EA+ C+KYLNRSVLEGR+IT
Sbjct: 61  KRELEKHFSAEGKVIDVHLVVDPWTRESRGFGFVTMETLEEADRCVKYLNRSVLEGRVIT 120

Query: 121 VEKARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRS 180
           VEKA+RRRGRTPTPG+YLGLRT+  +RRS S S RRSPSYSPYRRS SRS + SSDRSRS
Sbjct: 121 VEKAKRRRGRTPTPGKYLGLRTIRAQRRSPSDSPRRSPSYSPYRRSYSRSPY-SSDRSRS 180

Query: 181 RSRSIDQHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWS 240
           RS S D  RR    PYH R  SYS        Y  RY S   Y  H+SYSRS SPY R  
Sbjct: 181 RSYSPDYRRRKSNYPYHSRRRSYS-------PYYSRYRSYSRYDRHRSYSRSCSPYSRSP 240

Query: 241 ---RDRSYSPCD--HYSPDPY-YRRHRYRSVSRSLSPKSRNSR--HY---SRSYSPEPRR 300
              RDRSYSP D  + SPD + YRRH YRSVSR  SP  R  R  HY   SRS SP PRR
Sbjct: 241 VSIRDRSYSPYDSRYDSPDDHSYRRHHYRSVSRGASPDDRYYRRHHYRSVSRSASPRPRR 300

Query: 301 RTLRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHES- 360
           R+ R           SYSRS +P   RS     S S+SP R R S SRS   + +  +  
Sbjct: 301 RSRR-----------SYSRSATPVSKRSN----SRSVSP-RSRKSHSRSSKRSGKYEKHY 360

Query: 361 TRKRGYSSRSRSRSQSVTSRSVSPA 368
           +R    S+ SRS S+S T RS SP+
Sbjct: 361 SRSPSVSASSRSVSRSNTPRSTSPS 361

BLAST of Cucsa.146830 vs. TrEMBL
Match: A0A0B2NXN8_GLYSO (Putative RNA-binding protein C25G10.01 OS=Glycine soja GN=glysoja_028457 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 9.0e-104
Identity = 237/385 (61.56%), Postives = 264/385 (68.57%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSR--SRSRSRSPSSDVENPGNNLYVTGLSPRIT 60
           MSYSRRSRYSRSPS    + RSVSRSLSR  SRSRSRS S D ENPGNNLYVTGLSPRIT
Sbjct: 1   MSYSRRSRYSRSPSPYKRYSRSVSRSLSRTSSRSRSRSLSRDAENPGNNLYVTGLSPRIT 60

Query: 61  RRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIIT 120
           +RELEKHFSAEG V+DVHLV DP TRESRGFGF+TM + +EA+ C+KYLNRSVLEGR+IT
Sbjct: 61  KRELEKHFSAEGKVIDVHLVVDPWTRESRGFGFVTMETLEEADRCVKYLNRSVLEGRVIT 120

Query: 121 VEKARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRS 180
           VEKA+RRRGRTPTPG+YLGLRT+  +RRS S S RRSPSYSPYRRS SRS + SSDRSRS
Sbjct: 121 VEKAKRRRGRTPTPGKYLGLRTIRAQRRSPSDSPRRSPSYSPYRRSYSRSPY-SSDRSRS 180

Query: 181 RSRSIDQHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWS 240
           RS S D  RR    PYH R  SYS        Y  RY S   Y  H+SYSRS SPY R  
Sbjct: 181 RSYSPDYRRRKSNYPYHSRRRSYS-------PYYSRYRSYSRYDRHRSYSRSCSPYSRSP 240

Query: 241 ---RDRSYSPCD--HYSPDPY-YRRHRYRSVSRSLSPKSRNSR--HY---SRSYSPEPRR 300
              RDRSYSP D  + SPD + YRRH YRSVSR  SP  R  R  HY   SRS SP PRR
Sbjct: 241 VSIRDRSYSPYDSRYDSPDDHSYRRHHYRSVSRGASPDDRYYRRHHYRSVSRSASPRPRR 300

Query: 301 RTLRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHES- 360
           R+ R           SYSRS +P   RS     S S+SP R R S SRS   + +  +  
Sbjct: 301 RSRR-----------SYSRSATPVSKRSN----SRSVSP-RSRKSHSRSSKRSGKYEKHY 360

Query: 361 TRKRGYSSRSRSRSQSVTSRSVSPA 368
           +R    S+ SRS S+S T RS SP+
Sbjct: 361 SRSPSVSASSRSVSRSNTPRSTSPS 361

BLAST of Cucsa.146830 vs. TrEMBL
Match: A0A0R0J3B8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_08G334100 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 9.0e-104
Identity = 237/385 (61.56%), Postives = 264/385 (68.57%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSR--SRSRSRSPSSDVENPGNNLYVTGLSPRIT 60
           MSYSRRSRYSRSPS    + RSVSRSLSR  SRSRSRS S D ENPGNNLYVTGLSPRIT
Sbjct: 15  MSYSRRSRYSRSPSPYKRYSRSVSRSLSRTSSRSRSRSLSRDAENPGNNLYVTGLSPRIT 74

Query: 61  RRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIIT 120
           +RELEKHFSAEG V+DVHLV DP TRESRGFGF+TM + +EA+ C+KYLNRSVLEGR+IT
Sbjct: 75  KRELEKHFSAEGKVIDVHLVVDPWTRESRGFGFVTMETLEEADRCVKYLNRSVLEGRVIT 134

Query: 121 VEKARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRS 180
           VEKA+RRRGRTPTPG+YLGLRT+  +RRS S S RRSPSYSPYRRS SRS + SSDRSRS
Sbjct: 135 VEKAKRRRGRTPTPGKYLGLRTIRAQRRSPSDSPRRSPSYSPYRRSYSRSPY-SSDRSRS 194

Query: 181 RSRSIDQHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWS 240
           RS S D  RR    PYH R  SYS        Y  RY S   Y  H+SYSRS SPY R  
Sbjct: 195 RSYSPDYRRRKSNYPYHSRRRSYS-------PYYSRYRSYSRYDRHRSYSRSCSPYSRSP 254

Query: 241 ---RDRSYSPCD--HYSPDPY-YRRHRYRSVSRSLSPKSRNSR--HY---SRSYSPEPRR 300
              RDRSYSP D  + SPD + YRRH YRSVSR  SP  R  R  HY   SRS SP PRR
Sbjct: 255 VSIRDRSYSPYDSRYDSPDDHSYRRHHYRSVSRGASPDDRYYRRHHYRSVSRSASPRPRR 314

Query: 301 RTLRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHES- 360
           R+ R           SYSRS +P   RS     S S+SP R R S SRS   + +  +  
Sbjct: 315 RSRR-----------SYSRSATPVSKRSN----SRSVSP-RSRKSHSRSSKRSGKYEKHY 374

Query: 361 TRKRGYSSRSRSRSQSVTSRSVSPA 368
           +R    S+ SRS S+S T RS SP+
Sbjct: 375 SRSPSVSASSRSVSRSNTPRSTSPS 375

BLAST of Cucsa.146830 vs. TAIR10
Match: AT1G07350.1 (AT1G07350.1 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 286.6 bits (732), Expect = 2.2e-77
Identity = 204/381 (53.54%), Postives = 232/381 (60.89%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS-FKRSVSRSLSRSRSRS--RSPSSDVENPGNNLYVTGLSPRITRRE 60
           MSYSRRSRYS S S + +   RS+SRS SRS  RS SSD ENPGN+LYVTGLS R+T R+
Sbjct: 31  MSYSRRSRYSPSLSPYDKRRGRSVSRSLSRSPTRSVSSDAENPGNSLYVTGLSHRVTERD 90

Query: 61  LEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEK 120
           LE HF+ EG V DVHLV DP TRESRGFGFI+M S  +A  CI+ L+ SVL+GR+ITVEK
Sbjct: 91  LEDHFAKEGKVTDVHLVLDPWTRESRGFGFISMKSVGDANRCIRSLDHSVLQGRVITVEK 150

Query: 121 ARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSR 180
           ARRRRGRTPTPG+YLGLRT  GR +S SYS RRS S      S SRS   SSDR RS S 
Sbjct: 151 ARRRRGRTPTPGKYLGLRTARGRHKSPSYSPRRSVSC-----SRSRSRSYSSDRGRSYSP 210

Query: 181 SIDQHRRAYG-SPYHRRSYSYSRRRYDSPD--YRRRYDSPDYYRHHKSYSRSRSP-YRRW 240
           S  +  R+   SP++RR   YS  R  SPD  Y RR D            RS SP YRR 
Sbjct: 211 SYGRRGRSSSYSPFYRRRRFYSPSRSPSPDDRYNRRRD------------RSYSPYYRRR 270

Query: 241 SRDRSYS-PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRS 300
            R RSYS  C      PYY R RYRS SRS SP+ R +R  S S     R R+   HY+ 
Sbjct: 271 DRSRSYSRNCRARDRSPYYMR-RYRSRSRSYSPRYR-ARDRSCSPYYRGRDRSYSPHYQG 330

Query: 301 PSQELSSYSRSISPERSRSGS-----SIYSSSISPERGR-SSRSRSCSHTPRRHESTRKR 360
             +  S  SR     RS SGS        S SISP +GR  SRS+S     RRH+     
Sbjct: 331 RDRSYSPESRYYRRHRSVSGSVSPGGRSMSRSISPRKGRKESRSKS-----RRHDR---- 382

Query: 361 GYSSRSRSRS-QSVTSRSVSP 367
             SS   SRS +S TSRSVSP
Sbjct: 391 -QSSMCHSRSARSSTSRSVSP 382

BLAST of Cucsa.146830 vs. TAIR10
Match: AT4G35785.5 (AT4G35785.5 RNA-binding (RRM/RBD/RNP motifs) family protein)

HSP 1 Score: 125.2 bits (313), Expect = 8.5e-29
Identity = 73/131 (55.73%), Postives = 83/131 (63.36%), Query Frame = 1

Query: 2   SYSRRSRYSRSPSFKRSVSRSLSRSR---------------SRSRSPSSDVENPGNNLYV 61
           S SR    SRS S  R VS S SR R               SRSRS  S+VENPG  LYV
Sbjct: 31  SRSRPRLRSRSRSLPRPVSPSRSRGRILRGNLFFITCTLCRSRSRSRGSEVENPGTTLYV 90

Query: 62  TGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRS 118
           TGLS R+T ++LE HF+ EG V    LV +P TR SRGF F+TMSS  +AE CIKYLN+S
Sbjct: 91  TGLSTRVTDKDLEAHFAKEGKVASCFLVMEPRTRVSRGFAFVTMSSLKDAERCIKYLNQS 150


HSP 2 Score: 119.0 bits (297), Expect = 6.1e-27
Identity = 74/156 (47.44%), Postives = 88/156 (56.41%), Query Frame = 1

Query: 5   RRSRYSRSPSFKRSVSRSLSRSRSRSR----------------SPS-------------- 64
           +R R SRSPS ++  +RS SRSRSRSR                SPS              
Sbjct: 6   QRRRDSRSPSPRKERARSRSRSRSRSRSRPRLRSRSRSLPRPVSPSRSRGRILRGNLFFI 65

Query: 65  -------------SDVENPGNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRE 118
                        S+VENPG  LYVTGLS R+T ++LE HF+ EG V    LV +P TR 
Sbjct: 66  TCTLCRSRSRSRGSEVENPGTTLYVTGLSTRVTDKDLEAHFAKEGKVASCFLVMEPRTRV 125

BLAST of Cucsa.146830 vs. TAIR10
Match: AT5G64200.1 (AT5G64200.1 ortholog of human splicing factor SC35)

HSP 1 Score: 105.5 bits (262), Expect = 7.0e-23
Identity = 110/320 (34.38%), Postives = 142/320 (44.38%), Query Frame = 1

Query: 43  NLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKY 102
           +L V  ++ R T  +L   F+  G V+DV +  D  T +SRGF F+     DEA   ++ 
Sbjct: 17  SLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKDEAHKAVER 76

Query: 103 LNRSVLEGRIITVE---------------------KARRRRGRTPTPGRYLGLRTVHGRR 162
           L+  V++GR ITV+                     K+RR R R+P        R     R
Sbjct: 77  LDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRSP--------RRSRSPR 136

Query: 163 RSRSYSSRRSPSYSPYRRSSSRSTHCSSD-RSRSRSRSIDQHRRAYGSPY-HRRSYSYSR 222
           RSRS   RRSP  S   R  SR  +   D R RSRSRS D+  R       HRR    +R
Sbjct: 137 RSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRSYDRRERHEEKDRDHRRR---TR 196

Query: 223 RRYDSPDYRRRYDSPDYYRHHKSYSR--SRSPYRRWSRDRS---YSPCDHYSPDPYYRRH 282
            R  SPD +RR     Y    +S+SR  S SP RR  R  S    SP    SPD      
Sbjct: 197 SRSASPDEKRRVRG-RYDNESRSHSRSLSASPARRSPRSSSPQKTSPAREVSPDK----- 256

Query: 283 RYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSYSRSISPERSRSGSS 335
             RS  RS SP+        RS SP           RSP+ + +S S+ +SPER  +  S
Sbjct: 257 --RSNERSPSPR--------RSLSP-----------RSPALQKASPSKEMSPERRSNERS 298


HSP 2 Score: 61.6 bits (148), Expect = 1.2e-09
Identity = 62/185 (33.51%), Postives = 81/185 (43.78%), Query Frame = 1

Query: 190 YHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSP----CDHY 249
           Y   +   S+ R   P  + R       R  +S  RSRSP RR S  RS SP     D Y
Sbjct: 94  YGPNAEKISKGRVVEPPPKSRRSRSRSPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDY 153

Query: 250 SPDPYYRRHRYRSVSRSLSPKSRNSRH----YSRSYSPEPRRRTLRNHYRSPSQELSSYS 309
               Y +R R RS  R    + ++  H     SRS SP+ +RR +R  Y + S+   S+S
Sbjct: 154 REKDYRKRSRSRSYDRRERHEEKDRDHRRRTRSRSASPDEKRR-VRGRYDNESR---SHS 213

Query: 310 RSIS---PERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQS 364
           RS+S     RS   SS   +S + E     RS   S +PRR  S R       S S+  S
Sbjct: 214 RSLSASPARRSPRSSSPQKTSPAREVSPDKRSNERSPSPRRSLSPRSPALQKASPSKEMS 273

BLAST of Cucsa.146830 vs. TAIR10
Match: AT1G60650.1 (AT1G60650.1 RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc finger-like domain)

HSP 1 Score: 79.3 bits (194), Expect = 5.4e-15
Identity = 80/267 (29.96%), Postives = 112/267 (41.95%), Query Frame = 1

Query: 36  DVENPGN---NLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSS 95
           D EN GN    ++V GLS  +T R+LE  F   G + +  ++    T   RGFGFIT + 
Sbjct: 3   DRENDGNLESRIFVGGLSWDVTERQLESTFDRYGKITECQIMVGRDTGRPRGFGFITFTD 62

Query: 96  NDEAENCIKYLNRSVLEGRIITVEKARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSP 155
              A++ IK+++   L  ++I+V KA  +          +G   V   ++   YSSR   
Sbjct: 63  RRGADDAIKHMHGRELGNKVISVNKAEPK----------VGGEDVDQLKKGGGYSSRGKG 122

Query: 156 SYS---PYRRSSSRSTHCSS---DRS--------RSRSRSIDQHRRAYGSPYHRRS---- 215
           +       RR    +  C S   DR         RSR   ID HR  YG     R     
Sbjct: 123 TEDECFKCRRPGHWARDCPSTGDDRERFRVPLAMRSRIGDIDGHRDRYGDRDLERERERE 182

Query: 216 -----YSYSRRRYDSP--DYRRRYDSPDYY--RHHKSYSRSRSPYRRWSRDRSYSPCDHY 273
                Y   RR  D     YR R+DS D Y  R H  + R   P  R+  DR Y   +H+
Sbjct: 183 REFDRYMDGRRDRDGGRYSYRDRFDSGDKYEPRDHYPFERYAPPGDRFVSDR-YGMPEHH 242

BLAST of Cucsa.146830 vs. TAIR10
Match: AT5G04280.1 (AT5G04280.1 RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc finger-like domain)

HSP 1 Score: 70.5 bits (171), Expect = 2.5e-12
Identity = 86/316 (27.22%), Postives = 122/316 (38.61%), Query Frame = 1

Query: 41  GNNLYVTGLSPRITRRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCI 100
           G+ ++V GLSP +T R+LE+ FS  G +LD  ++ +  T  SRGFGFIT +     +  I
Sbjct: 6   GSRIFVGGLSPEVTDRDLERAFSRFGDILDCQIMLERDTGRSRGFGFITFADRRAMDESI 65

Query: 101 KYLNRSVLEGRIITVEKARRRRGRTPTPGRYLGLRTVHGRR--RSRSYSSRRSPSYSPYR 160
           + ++      R+I+V +A  + GR             HG R  R   YS     S+    
Sbjct: 66  REMHGRDFGDRVISVNRAEPKLGRDDGES--------HGSRGGRDSGYSIAGKGSFGGGG 125

Query: 161 RSS-----------SRSTHCSSD-RSRSRSR-----SIDQHRRAYGSP------YHRRSY 220
                          R  H + D  S    R            AYG        Y  R  
Sbjct: 126 GGGGRVGEDECFKCGRVGHWARDCPSAGGGRGGPVGGFSSRASAYGGSDGRVDRYADRDR 185

Query: 221 SYSRRRY--DSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYSPCDHYSPDPYYRR 280
              R RY  D  D   RY + D +   ++Y     P  R++ DR  +P D ++    Y R
Sbjct: 186 YVDRERYIDDRYDGAARYGARDRFDSREAY----IPRDRYASDRYAAPADRFAGGDRYSR 245

Query: 281 --HRY--------RSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSYSRS 320
              RY        RS  R ++P S  S  Y    +  P R          S+  + Y R 
Sbjct: 246 GSDRYPPGSYDKARSFERDIAP-SAGSDRYGGGRAGGPIRGGGEEGRGFRSRAGAPYER- 305

BLAST of Cucsa.146830 vs. NCBI nr
Match: gi|449455042|ref|XP_004145262.1| (PREDICTED: serine/arginine-rich splicing factor SR45a-like [Cucumis sativus])

HSP 1 Score: 741.5 bits (1913), Expect = 7.1e-211
Identity = 367/367 (100.00%), Postives = 367/367 (100.00%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK 60
           MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK
Sbjct: 1   MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK 60

Query: 61  HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR 120
           HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR
Sbjct: 61  HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR 120

Query: 121 RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID 180
           RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID
Sbjct: 121 RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID 180

Query: 181 QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS 240
           QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS
Sbjct: 181 QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS 240

Query: 241 PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSY 300
           PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSY
Sbjct: 241 PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQELSSY 300

Query: 301 SRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVT 360
           SRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVT
Sbjct: 301 SRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQSVT 360

Query: 361 SRSVSPA 368
           SRSVSPA
Sbjct: 361 SRSVSPA 367

BLAST of Cucsa.146830 vs. NCBI nr
Match: gi|659115137|ref|XP_008457405.1| (PREDICTED: serine/arginine-rich splicing factor 2-like [Cucumis melo])

HSP 1 Score: 687.6 bits (1773), Expect = 1.2e-194
Identity = 347/369 (94.04%), Postives = 356/369 (96.48%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK 60
           MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK
Sbjct: 1   MSYSRRSRYSRSPSFKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRRELEK 60

Query: 61  HFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR 120
           HFSAEGTVLDVHL+ADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR
Sbjct: 61  HFSAEGTVLDVHLLADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVEKARR 120

Query: 121 RRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRSRSID 180
           RRGRTPTPGRYLGLRTVH RRRSRSYSSRRSPSYSPYRRSSSRSTH SSDRSRSRSRSI+
Sbjct: 121 RRGRTPTPGRYLGLRTVHVRRRSRSYSSRRSPSYSPYRRSSSRSTHYSSDRSRSRSRSIN 180

Query: 181 QHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWSRDRSYS 240
           +HRR+YGSPYHRRSYSYSRRRYDSP YRRRYDSPDYYRHH+SYSRS SPYRRWSRDRSYS
Sbjct: 181 RHRRSYGSPYHRRSYSYSRRRYDSPYYRRRYDSPDYYRHHRSYSRSSSPYRRWSRDRSYS 240

Query: 241 PCDHYSPDPYYRRHRYRSVSRSLSPKSRNSRHYSRSYSPEPRRRTLRNHYRSPSQE-LSS 300
           P D+YSPDPYYRRHRYRSVSRSLSPKSRN RHYSRSYSPEPRRRTLRN+  SPS+E   S
Sbjct: 241 PYDYYSPDPYYRRHRYRSVSRSLSPKSRNGRHYSRSYSPEPRRRTLRNYSHSPSRESRRS 300

Query: 301 YSRSISPERS-RSGSSIYSSSISPERGRSSRSRSCSHTPRRHESTRKRGYSSRSRSRSQS 360
           YSRSISPERS RSGSS+YSSSISPERGRS RSRSCSHTPRRHESTRKRGYSSRSRSRSQS
Sbjct: 301 YSRSISPERSRRSGSSVYSSSISPERGRSLRSRSCSHTPRRHESTRKRGYSSRSRSRSQS 360

Query: 361 VTSRSVSPA 368
           VTSRSVSPA
Sbjct: 361 VTSRSVSPA 369

BLAST of Cucsa.146830 vs. NCBI nr
Match: gi|255558828|ref|XP_002520437.1| (PREDICTED: serine/arginine-rich splicing factor SR45a isoform X2 [Ricinus communis])

HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-107
Identity = 241/397 (60.71%), Postives = 273/397 (68.77%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRR 60
           MSYS+RSRYS SPS    + RSVSRS+SRSRSRSRSPSSDVENPGNNLYVTGLSPRIT+R
Sbjct: 2   MSYSKRSRYSPSPSPYRRYSRSVSRSMSRSRSRSRSPSSDVENPGNNLYVTGLSPRITKR 61

Query: 61  ELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVE 120
           +LEKHF++EG V+DVHLV DP TRESRGFGF+TMS+  EA+ CIKYLNRSVLEGR+ITVE
Sbjct: 62  DLEKHFASEGKVIDVHLVVDPWTRESRGFGFVTMSTLVEADRCIKYLNRSVLEGRVITVE 121

Query: 121 KARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRSRS 180
           KA+RRRGRTPTPGRYLGLRT+  RR + S+S  RSPSYS YR S SRS   SS+RSRSRS
Sbjct: 122 KAKRRRGRTPTPGRYLGLRTIRVRRLTPSHSPHRSPSYSRYRGSLSRSPRYSSERSRSRS 181

Query: 181 RSIDQHRRA--------YGSPYHRRSYS--YSRRRYDSPDYR-RRYDSPDYYRHHKSYSR 240
            S    RR+        YG     RSYS  Y RRR  SP YR RR  SP YY  H+SYSR
Sbjct: 182 YSPHYRRRSRSRSFSPSYGRRSRSRSYSPYYRRRRSYSPYYRSRRSYSPYYYSRHRSYSR 241

Query: 241 SRSPYRR---WSRDRSYSPCDHYSPDP---YYRRHRYRSVSRSLSPKSRNS-RHYSRSYS 300
           SRSPY R      DRSYSP       P   YYRRHR+RSVS S   ++R S R YSRS S
Sbjct: 242 SRSPYSRSPVSRHDRSYSPYGSRYDSPGERYYRRHRHRSVSSSPGRRTRRSARSYSRSIS 301

Query: 301 PEPRRRTLRNHYRS----PSQELSSYSRSISPERSRSGSSIYSSSISPER---GRSSRSR 360
           P PRR + R++  S    P     SYSRS SP   +S    YS SISP R    R SRSR
Sbjct: 302 PSPRRSSRRSYSSSRSPPPRSSRKSYSRSPSPRMRKSLKRSYSRSISPIRRKSSRRSRSR 361

Query: 361 SCSHTPRRHES-TRKRGYSSRSRSRSQSVTSRSVSPA 368
           S S      ++ +R R  S+ SRS S+S T RS SP+
Sbjct: 362 SASSVHDSQDTYSRSRNLSASSRSVSRSPTPRSASPS 398

BLAST of Cucsa.146830 vs. NCBI nr
Match: gi|1000963365|ref|XP_015575505.1| (PREDICTED: serine/arginine-rich splicing factor SR45a isoform X1 [Ricinus communis])

HSP 1 Score: 396.7 bits (1018), Expect = 4.3e-107
Identity = 242/399 (60.65%), Postives = 274/399 (68.67%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSRSRSRSRSPSSDVENPGNNLYVTGLSPRITRR 60
           MSYS+RSRYS SPS    + RSVSRS+SRSRSRSRSPSSDVENPGNNLYVTGLSPRIT+R
Sbjct: 2   MSYSKRSRYSPSPSPYRRYSRSVSRSMSRSRSRSRSPSSDVENPGNNLYVTGLSPRITKR 61

Query: 61  ELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIITVE 120
           +LEKHF++EG V+DVHLV DP TRESRGFGF+TMS+  EA+ CIKYLNRSVLEGR+ITVE
Sbjct: 62  DLEKHFASEGKVIDVHLVVDPWTRESRGFGFVTMSTLVEADRCIKYLNRSVLEGRVITVE 121

Query: 121 KARRRRGRTPTPGRYLGLRTVHG--RRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRS 180
           KA+RRRGRTPTPGRYLGLRT+ G  RR + S+S  RSPSYS YR S SRS   SS+RSRS
Sbjct: 122 KAKRRRGRTPTPGRYLGLRTIRGKLRRLTPSHSPHRSPSYSRYRGSLSRSPRYSSERSRS 181

Query: 181 RSRSIDQHRRA--------YGSPYHRRSYS--YSRRRYDSPDYR-RRYDSPDYYRHHKSY 240
           RS S    RR+        YG     RSYS  Y RRR  SP YR RR  SP YY  H+SY
Sbjct: 182 RSYSPHYRRRSRSRSFSPSYGRRSRSRSYSPYYRRRRSYSPYYRSRRSYSPYYYSRHRSY 241

Query: 241 SRSRSPYRR---WSRDRSYSPCDHYSPDP---YYRRHRYRSVSRSLSPKSRNS-RHYSRS 300
           SRSRSPY R      DRSYSP       P   YYRRHR+RSVS S   ++R S R YSRS
Sbjct: 242 SRSRSPYSRSPVSRHDRSYSPYGSRYDSPGERYYRRHRHRSVSSSPGRRTRRSARSYSRS 301

Query: 301 YSPEPRRRTLRNHYRS----PSQELSSYSRSISPERSRSGSSIYSSSISPER---GRSSR 360
            SP PRR + R++  S    P     SYSRS SP   +S    YS SISP R    R SR
Sbjct: 302 ISPSPRRSSRRSYSSSRSPPPRSSRKSYSRSPSPRMRKSLKRSYSRSISPIRRKSSRRSR 361

Query: 361 SRSCSHTPRRHES-TRKRGYSSRSRSRSQSVTSRSVSPA 368
           SRS S      ++ +R R  S+ SRS S+S T RS SP+
Sbjct: 362 SRSASSVHDSQDTYSRSRNLSASSRSVSRSPTPRSASPS 400

BLAST of Cucsa.146830 vs. NCBI nr
Match: gi|947097858|gb|KRH46443.1| (hypothetical protein GLYMA_08G334100 [Glycine max])

HSP 1 Score: 385.2 bits (988), Expect = 1.3e-103
Identity = 237/385 (61.56%), Postives = 264/385 (68.57%), Query Frame = 1

Query: 1   MSYSRRSRYSRSPS----FKRSVSRSLSR--SRSRSRSPSSDVENPGNNLYVTGLSPRIT 60
           MSYSRRSRYSRSPS    + RSVSRSLSR  SRSRSRS S D ENPGNNLYVTGLSPRIT
Sbjct: 15  MSYSRRSRYSRSPSPYKRYSRSVSRSLSRTSSRSRSRSLSRDAENPGNNLYVTGLSPRIT 74

Query: 61  RRELEKHFSAEGTVLDVHLVADPLTRESRGFGFITMSSNDEAENCIKYLNRSVLEGRIIT 120
           +RELEKHFSAEG V+DVHLV DP TRESRGFGF+TM + +EA+ C+KYLNRSVLEGR+IT
Sbjct: 75  KRELEKHFSAEGKVIDVHLVVDPWTRESRGFGFVTMETLEEADRCVKYLNRSVLEGRVIT 134

Query: 121 VEKARRRRGRTPTPGRYLGLRTVHGRRRSRSYSSRRSPSYSPYRRSSSRSTHCSSDRSRS 180
           VEKA+RRRGRTPTPG+YLGLRT+  +RRS S S RRSPSYSPYRRS SRS + SSDRSRS
Sbjct: 135 VEKAKRRRGRTPTPGKYLGLRTIRAQRRSPSDSPRRSPSYSPYRRSYSRSPY-SSDRSRS 194

Query: 181 RSRSIDQHRRAYGSPYHRRSYSYSRRRYDSPDYRRRYDSPDYYRHHKSYSRSRSPYRRWS 240
           RS S D  RR    PYH R  SYS        Y  RY S   Y  H+SYSRS SPY R  
Sbjct: 195 RSYSPDYRRRKSNYPYHSRRRSYS-------PYYSRYRSYSRYDRHRSYSRSCSPYSRSP 254

Query: 241 ---RDRSYSPCD--HYSPDPY-YRRHRYRSVSRSLSPKSRNSR--HY---SRSYSPEPRR 300
              RDRSYSP D  + SPD + YRRH YRSVSR  SP  R  R  HY   SRS SP PRR
Sbjct: 255 VSIRDRSYSPYDSRYDSPDDHSYRRHHYRSVSRGASPDDRYYRRHHYRSVSRSASPRPRR 314

Query: 301 RTLRNHYRSPSQELSSYSRSISPERSRSGSSIYSSSISPERGRSSRSRSCSHTPRRHES- 360
           R+ R           SYSRS +P   RS     S S+SP R R S SRS   + +  +  
Sbjct: 315 RSRR-----------SYSRSATPVSKRSN----SRSVSP-RSRKSHSRSSKRSGKYEKHY 374

Query: 361 TRKRGYSSRSRSRSQSVTSRSVSPA 368
           +R    S+ SRS S+S T RS SP+
Sbjct: 375 SRSPSVSASSRSVSRSNTPRSTSPS 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SR45A_ARATH3.9e-7653.54Serine/arginine-rich splicing factor SR45a OS=Arabidopsis thaliana GN=SR45A PE=1... [more]
YDC1_SCHPO1.1e-2232.60Uncharacterized RNA-binding protein C25G10.01 OS=Schizosaccharomyces pombe (stra... [more]
SC35_ARATH1.2e-2134.38Serine/arginine-rich splicing factor SC35 OS=Arabidopsis thaliana GN=SC35 PE=1 S... [more]
SRSF2_PIG1.7e-1838.21Serine/arginine-rich splicing factor 2 OS=Sus scrofa GN=SRSF2 PE=2 SV=1[more]
SRSF2_HUMAN2.2e-1838.21Serine/arginine-rich splicing factor 2 OS=Homo sapiens GN=SRSF2 PE=1 SV=4[more]
Match NameE-valueIdentityDescription
A0A0A0LXJ6_CUCSA5.0e-211100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528490 PE=4 SV=1[more]
B9S368_RICCO1.0e-10760.71FUS-interacting serine-arginine-rich protein 1, putative OS=Ricinus communis GN=... [more]
A0A0R0IW62_SOYBN9.0e-10461.56Uncharacterized protein OS=Glycine max GN=GLYMA_08G334100 PE=4 SV=1[more]
A0A0B2NXN8_GLYSO9.0e-10461.56Putative RNA-binding protein C25G10.01 OS=Glycine soja GN=glysoja_028457 PE=4 SV... [more]
A0A0R0J3B8_SOYBN9.0e-10461.56Uncharacterized protein OS=Glycine max GN=GLYMA_08G334100 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G07350.12.2e-7753.54 RNA-binding (RRM/RBD/RNP motifs) family protein[more]
AT4G35785.58.5e-2955.73 RNA-binding (RRM/RBD/RNP motifs) family protein[more]
AT5G64200.17.0e-2334.38 ortholog of human splicing factor SC35[more]
AT1G60650.15.4e-1529.96 RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc... [more]
AT5G04280.12.5e-1227.22 RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc... [more]
Match NameE-valueIdentityDescription
gi|449455042|ref|XP_004145262.1|7.1e-211100.00PREDICTED: serine/arginine-rich splicing factor SR45a-like [Cucumis sativus][more]
gi|659115137|ref|XP_008457405.1|1.2e-19494.04PREDICTED: serine/arginine-rich splicing factor 2-like [Cucumis melo][more]
gi|255558828|ref|XP_002520437.1|1.5e-10760.71PREDICTED: serine/arginine-rich splicing factor SR45a isoform X2 [Ricinus commun... [more]
gi|1000963365|ref|XP_015575505.1|4.3e-10760.65PREDICTED: serine/arginine-rich splicing factor SR45a isoform X1 [Ricinus commun... [more]
gi|947097858|gb|KRH46443.1|1.3e-10361.56hypothetical protein GLYMA_08G334100 [Glycine max][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
IPR000504RRM_dom
IPR012677Nucleotide-bd_a/b_plait_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
GO:0003676nucleic acid binding
GO:0000166nucleotide binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043484 regulation of RNA splicing
biological_process GO:0009644 response to high light intensity
biological_process GO:0008150 biological_process
cellular_component GO:0030529 intracellular ribonucleoprotein complex
cellular_component GO:0019013 viral nucleocapsid
cellular_component GO:0005575 cellular_component
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.146830.2Cucsa.146830.2mRNA
Cucsa.146830.1Cucsa.146830.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 44..113
score: 1.6
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 43..116
score: 2.0
IPR000504RNA recognition motif domainPROFILEPS50102RRMcoord: 42..120
score: 17
IPR012677Nucleotide-binding alpha-beta plait domainGENE3DG3DSA:3.30.70.330coord: 26..133
score: 1.2
IPR012677Nucleotide-binding alpha-beta plait domainunknownSSF54928RNA-binding domain, RBDcoord: 30..149
score: 1.16
NoneNo IPR availablePANTHERPTHR15241TRANSFORMER-2-RELATEDcoord: 348..356
score: 1.3E-74coord: 1..165
score: 1.3E-74coord: 195..254
score: 1.3
NoneNo IPR availablePANTHERPTHR15241:SF4TRANSFORMER-SR RIBONUCLEOPROTEIN-RELATEDcoord: 348..356
score: 1.3E-74coord: 1..165
score: 1.3E-74coord: 195..254
score: 1.3

The following gene(s) are paralogous to this gene:

None