Cucsa.143280 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.143280
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	E3 ubiquitin ligase BIG brother-like protein
Location	scaffold01079 : 1619555 .. 1625528 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCTAAATTGGCCGAAGAAACCTACAGAAACCAAACCAACCACAATCATTCAACCATAGACTCTCTCTCTCTTTTTTCCCATCAAAGTTGATCATTGTCTCTTTCTTTCTTTAATCCCTTCCCTCTTCCTTTCGAATCAATCCCCTTTTCCTTTTCCTTTTCTTCTTCTTTTTTTTCTCTTCTCCCATTTCATTTTCATCTAGTTTTCCGGCACCCCTTTTGTTTTTTTCCTTCATCTCTTTCACTTCTTCCTCCAATTTCGATTTTTTCTGCTGACCCACATGTTCTTTTGAGCTTTTCATTCTTGGGATTCGGCGTCGGAGCTGCTTGTTTGTCTTCTGCGGATCGCTCCGTCGTTGAAATGTGAGTTTCTAACCACCTACATTTTGGGTTTTCTTGTTTTGCTTCTGTTTGTGTTGAGGCGAGTGGTGGTGGGATTCCTTTGATTGTGTTTAATTTTGATTGAATAAGTGATATTTGGGGTTGAATGATTGCTTGTTTCATTTCCTTTTTTTTTCCAGCAATTGCGAAATGTAGAAGGGATTGTTAGGGAGTTTGAGTCCGATGGTACTCTTCCATTGTGTTTCCTTCGTTAATTGTTGCTTGGTTTTAAAGAATTTGGATTTTATCTATTTCTTTTTCTGGGATCTGGTAGACTAGAGATTTGCTGAGAAGTGGTTTTGGCTGTGGCAGATGGCTTTAGACGAAATGTCTCGTCTGATCTGTGTATATTGTGTATGGGTTTACTCTACTTTAATCTAGGACATTGGAAAACTTTAGGGGCTGATTTTTCATGATCTGTTTCTCACATTTAGGTGTAAAATAATACTTGGTTTGCTTATTTTTCTCTTCTGTTTCAAGTTGTATGTTGGAACCTCTAACGGTTTAGTTTGGTAAACTTTTGTAATGGGTTGGCTTTGCTGAACAGTTTGGAGTGGGAGTTTGTTCTTTTAGTTTATTGCCTGGAGTATTCATTTGGTTTTGTGATGTTTCTGCATTTTACAGAATATTTGTTAACTTCAGTGTTAACTTCAGTTGTGTATGAAATGAGTTGGAACAGCACGCCGCAGACAGAAGTTCATCATGTTAACACCAATTATCCTTATAACACTGCTGGAAGTTTTCTGGAATACTTTGAAGGACTTACCTATGAACATGTAAATTTCATTTTTTCGGGTGCTTCACATTCTCAGGTACTTTAAATCTGCTTGCTTCTGTGTTCCAAACAACTGAACTTGAATTTTTTCCTTGCTATTGAACTATTCAATTGATTATTTATTTATTTTTATTTTTTTAATCTTCTCTGATGTAACTTTACGGACATCTACTTTACAGCACATGATTATTTTCTTGTTTGTGAAATGAAAGGTTGTGGTATATGCATCTTTTTGAGGGGCAGTAACATTTATTCTATAGGGAGTGATCTTGGGAAAGTTTCTTTGCATTTCTTTGGTTTTTTCTTCGTCTGTCCTTCCTTCTTGGGTTTTCTTTCAAGTTGAAATTGTCTTCCTCTCTTTATAATTTATAAGCTGGCAAATACTATACTTGAACGTAGTGCTCCTCTCTACCGTTGCTTTAATTAGTTTTGACTGTGAATGGAAAATGGCAATATACTTATTAAGTTTCACACTTGCAGGAGAATGTTTATCCTCCACTGAATTCAAACTTTTACAAGTTTGGGTTTTCAGATTTTGGGAGTCCTTCCTATTACAACCCACCTCAAGCTTATAGGATGCATGATCACGAGCCCATAATTTATGAACATAGAAGGCTAGAAAATTCTGCAACTCAGCCTAATTTACAGAGTACCGTAAATCCTGAATTCGAAGAAATTACAAATACTATAGCATTTGACAACCATGTAGAATGTAAGTGATGATTCTCGGTAATTAGATAATTCTCACCATTCTCATCCTCCCCCCCCCCCCCAAAAAAAAAAAAAAAAAGAAAGGAAAGAAAAGAAAAGAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGGAAAGAAAAGAAAAGAAAAAAAAGAGTTCGTTTCAATTTTTTTTGTATTTGTTGGGTTCACCATTGAGATTTCCTAGAAGTACGCAAAATATTTACTTTTGCAAGTGTAACAATTGTCTTGGTAGGAGTGAAACACCAAACACTGAAGGGGTTTTTTTCTAATATAAAAACGTAATTTTATCATTAACATTTGCCAGCATAGGGAATATCAGTATCTTTGTACTTATTTCTCAAGAGTTCAGTGCAGGCTGAATGTTACATCTTGAGAGAACACTTCATACATGAATGAACTAAGAATTCTGGTAGAAGATTCTTATAAGTTTTGTTTAGTTAAATACCAAGAAGAAATATGGTTCTTGAAATAAGATGACCCTTGAATTTTAGCTATTGGTTTATCTTTAAATGCTGCATATTTTCCCTCTCTGTTAGCACTTTTTTCGACTCTTGCTGTTAGCACTAACAACAGATTTTCTCTCAGGTCCAAGGAGACATCATAATTCACATGATTTTCAGGTTTGTGGCTTTTTACATGGTTTGACAAGTCAACTTTCTACTTGAAATACGACACTTGTTTGGTCGTGTGTTTTGGCCAAAATTATTGATGAACGAATATTTATGTTTACGTGTAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTATGAGGTTAGTGCATTTGATAAACTTTTTGTGTACTTGTTTTTTCCTTTTATTTGTTGGTGAAAGTATCGATTTCCATCAATATATGAAACCACATAGTCGGAAGAACCAAGTAATGCAAGTTACAATGATTGTGTCATCTCCTCAATTTATGGGGACGAATAAACCATGGGAGTTTTAATATCAATTTTATGATTGATTAGCAGATGCTGAAAGCTACCTTTGATATTGCATAAGAATTTAAACTTGATGTAATAGAATAACTGTAAGGAACTTGGGGAAAAAATTCTTGGTTGAGGATAGGCCAGAATGAGATGATTGATGAGACATTAAAATATGTAAGCTTAAGAAAGTTGTTCTTCCAAACCAAGCATAGTCAGCTGGTATCCTCAACCTAGAGATCAGAGGTTCAAATCTCTTGCCCCATGTTATTGAACTAAAAAAATTAGTTTAATAAGGATCCTGGTGCTTTATGATAGAGCTGCTGAACTATTATTCTGGGGTCAAGTTTTTATGTTTGTTTTTTGAACTAATGTTAAACAAATTTCTCCATCTGTCATCTGACCTAATCGTCCAAAATGTATGTTTATCTTTTCTCAATATTTGAATAAAAATCAATAATATTTCAAGTTCAGGAATTACTAGAGTTGGGTGAGACAGTTGGAACTCAAAGCCGAGGTCTATCTCAAGAATTGATCGCTTTGCTTCCGATATCGAAGTACAAATATAGTTTCTTCTCAAGGAAAAAGTCACGAGGAGAGAGGTATATCTTAGCTTATAATGTGCATTTGATGGAATGTGTTAAACTTTTTTCCTTTCCTTTCCTTTCGTTGCCACTAACATTGAGCTGTAATCATTATCCTCACATTTTAGTACTTAAGTAGTGGTGTTGGCAAGTTAATTTCTTAGTGATACAAGTTCTCTGACGAGCGAGACTTTGTAGCCTGTTTTATGCTAGTTTCATCTTGAATCAGTGATACGGGAAACAAAATTTGATTATCAGGCATTCAGATATGGCTTTCTTTATAAAAAAGGGGGCATTCTGATAATTTCATGTCAGACATACGTCTAAACTATGTTTTACTCATTCTTTCGCTCCATCAAGACAAGAAAGAGAATGGCAGCATGTATATTTTTGTCTCTGAATTGATGATTTATTAGGCTTATTCCCATGATAAAAGACTTATCAATTTATGAGAGCTCACAATTGCCTTTGACGTTTGATAGATTGCCATACTCTACTGAAAGATCTTTTTTATCTTTTTTTTTTGAGATATGTTATCCAAGTATTCAAATAACAATATGTACCTTAACTTCTAAATTATGTCTTGTACTTGCGGAAAGATGCTAAATGAACATTTTGGACTTGGGCGTTCCTATTATAAAAAACTGAAACTGGTTGAGAGTTCAGTTTGTTCTTTTTCCTTTTGAGTCTAACTTTGTTCCTCTTAATTGGATGTAGATGTGTGATATGCCAGATGGAATATAAACGAGGTGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCTGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGTATTATTATTAGTTCATTGCATGTGCTGGGATTGTTGATTGTTTTATTGGTTACAACTCGCAGACTGATTCTTACGCTGTTCAAGATATCATAACAAGTAATAACCATGTTCATTCTGCCGAGGTCTTAAAAACGGCTTTATTAGAAACAAAATTCTAAAAGCTACTTTCCCATTTCCAAACCAGGGCTTGTTTTATTTGTGTTTATAAGCTTAACTTTCAAAAATAAAAGACAAAAAGTCAAATTGTTGTCAAATGAGGCCTTAATTTGGTTTTTGGTTTTGAAAATTAAGCTTCTAAAAACTACTTCCACTTCAAGATTTCTTGGTTATGTGATTTGCTTTTTATAGATGTTTTTAAAGTCCAAATTCAGTTTAAAAAATTAAGGCTTTTTTGGATAATCTTTTTTTTCTTTTTTTTTTTTTTGGTTTTTTTGAAAATTTCTCCCAATTTTTTAGTCATGATTTTCATATTTTTGTTTATGTAATTGTATAAATTCTTAGCCAATTTCTAGAAAACGAAACAATTTCTGAAACCGCTTCCTTTTAGTTTTCAGGTTTTAGATTTGATTTTGAAAAGGCTCCTACAATAGATAAAACAAACCATAAAAACCAATAGATGGAACTTGGAAGTGGTGCCTATAGGTTTAATTGTGGAGGTTACCGAACAAAACTTAAATAAAGTAGTTTTTGTCTTTGAAAGTTGATTAACAATTCAAATGTTCCTCAAATGTTACTCAAGGATTAGAAAACCACGATAAATAAATGGTGAGAAAGTGAATACAATTTTCAAAAAACCAAAAACCAAAAGGGACTCAAAATTTCTGATAGAGCTATTTTTTAAATGACTTGTAAAGGCTGAAGTTATTATGCACTTAGATCATTGTTTAGAGCTCCACAATCTTCTAAAGAAACCAATGAAAAACAACTTTCAGAAAACAATTTTGAAACCCAACTCTGAAGCAACTTCAGTTCCTGTCTTTAATAAAGTAAGGATGAAGATGATCTTTAATTGATTCTTCACCTTTTCTTTTTGGTGAGGCAGATCCAGAAACAGTCTTTTTGATTGAGTAATTAATTTCTATTGTATGGTTTTGTTGTACAGGCCTGCCCCATATGTTACACAGAGGTATTTGGTGATACTTCAAAACGCTAAGTCTTCTTCTCACTTCACTGTTAGATGACAGAAGAACATAATCTTTGTTATTGTTCATTATTTTTAAAATTTTTGTGAAAAAGAAAGAAAGAAAGAATATATATATATATTTTTATATATTTATATATATGGAGTGGCGCAAGGCAATCACTTGATTTTTCTTTCTTAAATATTTCCACTTTAGTAATATGGCAGTGCTGAAGTGAATAAAGATTAATTCTCCTTGTATTAGCTTTTATCAGTGTTTGGTGTTTGGATTGGCT

mRNA sequence

ATCTAAATTGGCCGAAGAAACCTACAGAAACCAAACCAACCACAATCATTCAACCATAGACTCTCTCTCTCTTTTTTCCCATCAAAGTTGAtcattgtctctttctttctttaatcccttccctcttcctttcgaatcaatccccttttccttttccttttcttcttcttttttttctcttctcccatttcattttcatctagttttccggcaccccttttgtttttttccttcatctctttcacttcttcctccAATTTCGATTTTTTCTGCTGACCCACATGTTCTTTTGAGCTTTTCATTCTTGGGATTCGGCGTCGGAGCTGCTTGTTTGTCTTCTGCGGATCGCTCCGTCGTTGAAATTTGTGTATGAAATGAGTTGGAACAGCACGCCGCAGACAGAAGTTCATCATGTTAACACCAATTATCCTTATAACACTGCTGGAAGTTTTCTGGAATACTTTGAAGGACTTACCTATGAACATGTAAATTTCATTTTTTCGGGTGCTTCACATTCTCAGGAGAATGTTTATCCTCCACTGAATTCAAACTTTTACAAGTTTGGGTTTTCAGATTTTGGGAGTCCTTCCTATTACAACCCACCTCAAGCTTATAGGATGCATGATCACGAGCCCATAATTTATGAACATAGAAGGCTAGAAAATTCTGCAACTCAGCCTAATTTACAGAGTACCGTAAATCCTGAATTCGAAGAAATTACAAATACTATAGCATTTGACAACCATGTAGAATGTCCAAGGAGACATCATAATTCACATGATTTTCAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTATGAGGAATTACTAGAGTTGGGTGAGACAGTTGGAACTCAAAGCCGAGGTCTATCTCAAGAATTGATCGCTTTGCTTCCGATATCGAAGTACAAATATAGTTTCTTCTCAAGGAAAAAGTCACGAGGAGAGAGATGTGTGATATGCCAGATGGAATATAAACGAGGTGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCTGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGCCTGCCCCATATGTTACACAGAGGTATTTGGTGATACTTCAAAACGCTAAGTCTTCTTCTCACTTCACTGTTAGATGACAGAAGAACATAATCTTTGTTATTGTTCATTATTTTTAAAATTTTTGTGAAAAAGAAAGAAAGAAAGAatatatatatatatttttatatatttatatatatGGAGTGGCGCAAGGCAATCACTTGATTTTTCTTTCTTAAATATTTCCACTTTAGTAATATGGCAGTGCTGAAGTGAATAAAGATTAATTCTCCTTGTATTAGCTTTTATCAGTGTTTGGTGTTTGGATTGGCt

Coding sequence (CDS)

ATGAGTTGGAACAGCACGCCGCAGACAGAAGTTCATCATGTTAACACCAATTATCCTTATAACACTGCTGGAAGTTTTCTGGAATACTTTGAAGGACTTACCTATGAACATGTAAATTTCATTTTTTCGGGTGCTTCACATTCTCAGGAGAATGTTTATCCTCCACTGAATTCAAACTTTTACAAGTTTGGGTTTTCAGATTTTGGGAGTCCTTCCTATTACAACCCACCTCAAGCTTATAGGATGCATGATCACGAGCCCATAATTTATGAACATAGAAGGCTAGAAAATTCTGCAACTCAGCCTAATTTACAGAGTACCGTAAATCCTGAATTCGAAGAAATTACAAATACTATAGCATTTGACAACCATGTAGAATGTCCAAGGAGACATCATAATTCACATGATTTTCAGGTTGTTTGGCAAGACAATGTAGATCCTGATAATATGACTTATGAGGAATTACTAGAGTTGGGTGAGACAGTTGGAACTCAAAGCCGAGGTCTATCTCAAGAATTGATCGCTTTGCTTCCGATATCGAAGTACAAATATAGTTTCTTCTCAAGGAAAAAGTCACGAGGAGAGAGATGTGTGATATGCCAGATGGAATATAAACGAGGTGATCGACGGATCACTCTGCCGTGCAAACATATCTACCATGCTGGTTGTGGAACCAAATGGCTTAGCATCAACAAGGCCTGCCCCATATGTTACACAGAGGTATTTGGTGATACTTCAAAACGCTAA

Protein sequence

MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNFYKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTSKR*

Homology

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BLAST of Cucsa.143280 vs. Swiss-Prot
Match: BB_ARATH (E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.7e-57
Identity = 113/253 (44.66%), Postives = 163/253 (64.43%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           M+ ++ P  + H+  T +PY   GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPII------YEHRRLENSATQPNLQSTVNPEFEE 120
           YKFGFS   + S+Y    +Y M+DH   +      +++  + N A  P  ++TV    + 
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGRTNWDYHPMVNVADDP--ENTVARSVQ- 120

Query: 121 ITNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELI 180
           I +T       EC    H+    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI
Sbjct: 121 IGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELI 180

Query: 181 ALLPISKYKY-SFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKA 240
             LP  KYK+ S FSRK++ GERCVICQ++YK G+R++ LPCKH+YH+ C +KWLSINK 
Sbjct: 181 ETLPTKKYKFGSIFSRKRA-GERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV 240

Query: 241 CPICYTEVFGDTS 247
           CP+C +EVFG+ S
Sbjct: 241 CPVCNSEVFGEPS 246

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BLAST of Cucsa.143280 vs. Swiss-Prot
Match: BBR_ARATH (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana GN=BBR PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.2e-25
Identity = 56/113 (49.56%), Postives = 71/113 (62.83%), Query Frame = 1


Query: 134 SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSR 193
           SH  Q  W D +DPD ++YEELL LG+ VGT+SRGLS + IA LP  +YK      +   
Sbjct: 228 SHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGT 287

Query: 194 GERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTS 247
            E CVIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 288 NESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

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BLAST of Cucsa.143280 vs. Swiss-Prot
Match: MBR1_ARATH (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.5e-15
Identity = 45/100 (45.00%), Postives = 58/100 (58.00%), Query Frame = 1


Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKK----SRGERCVIC 204
           +D DNM+YEELL LGE +G  S GLS+E+I L  + ++K++  S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 205 QMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 241
           Q EY  GD   TL C H +H  C  +W+ I   CPIC TE
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

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BLAST of Cucsa.143280 vs. Swiss-Prot
Match: MBR2_ARATH (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana GN=MBR2 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 9.9e-15
Identity = 42/96 (43.75%), Postives = 58/96 (60.42%), Query Frame = 1


Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSRG-ERCVICQME 204
           +D DNM+YEELL LGE +G  S GLS+E+I L  + ++K++  +    +  E C +CQ E
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKVMKQHKHTSSAAGSHQDMEPCCVCQEE 626

Query: 205 YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYT 240
           Y  GD   TL C H +H  C  +WL +   CPIC T
Sbjct: 627 YAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

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BLAST of Cucsa.143280 vs. Swiss-Prot
Match: HIP1_ORYSJ (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica GN=HIP1 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.7e-14
Identity = 43/94 (45.74%), Postives = 56/94 (59.57%), Query Frame = 1


Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKS-RGERCVICQME 204
           +D DNM+YEELL L E +G  S GLS+E +  L + + K+S +  + S   E C ICQ E
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKL-LKQRKFSSWRLEASVEEEPCCICQEE 627

Query: 205 YKRGDRRITLPCKHIYHAGCGTKWLSINKACPIC 238
           Y  GD   TL C H +H GC  +WL +   CPIC
Sbjct: 628 YVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

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BLAST of Cucsa.143280 vs. TrEMBL
Match: A0A0A0LX98_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G613470 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 1.1e-148
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

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BLAST of Cucsa.143280 vs. TrEMBL
Match: A0A067F1T9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025212mg PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.0e-99
Identity = 174/249 (69.88%), Postives = 204/249 (81.93%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN  P  EVH+ NT+YPYN+AGSF+EYFEGLTYEHVNFIF GASH QE+VYP  +++F
Sbjct: 1   MSWN--PHIEVHYYNTSYPYNSAGSFMEYFEGLTYEHVNFIFDGASHVQESVYPSTHTSF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQST--VNPEFEEITNT 120
           YKFG SD+GS SYY+    Y ++ HEP   E+RR   + ++ N + T  VN   E   NT
Sbjct: 61  YKFGLSDYGSNSYYDYGHPYEVNYHEPRNDEYRRPSENTSRSNNEQTAAVNAVPERNMNT 120

Query: 121 IAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLP 180
              ++ +ECPRRH N+H+ QVVWQDN+DPD MTYEELLELGETVGTQSRGLSQELI+LLP
Sbjct: 121 TTRESPIECPRRHQNTHEHQVVWQDNIDPDQMTYEELLELGETVGTQSRGLSQELISLLP 180

Query: 181 ISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY 240
           ISKYK  FF+RK+SR ERCVICQMEYKRGDRR+TLPCKH+YHAGCGT+WLSINKACPICY
Sbjct: 181 ISKYKCGFFTRKRSRNERCVICQMEYKRGDRRMTLPCKHVYHAGCGTRWLSINKACPICY 240

Query: 241 TEVFGDTSK 248
           TEVFGD+SK
Sbjct: 241 TEVFGDSSK 247

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BLAST of Cucsa.143280 vs. TrEMBL
Match: A0A061GTY3_THECC (RING/U-box superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_040623 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 2.7e-99
Identity = 175/249 (70.28%), Postives = 209/249 (83.94%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN  P  EV ++N++YPYN+AGSF+EYFEGLTY+HVNFIF GASH QE+VYP + S+F
Sbjct: 1   MSWN--PHMEVQYINSSYPYNSAGSFMEYFEGLTYQHVNFIFDGASHVQESVYPSMTSSF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRL-ENSATQPNLQST-VNPEFEEITNT 120
           YKFG SD G+ SYY+    Y +++HE  + E+RR  ENS++  N Q+  +N E+E   NT
Sbjct: 61  YKFGLSDSGNISYYDHGHNYEVNNHELCVDEYRRASENSSSMSNEQTAAMNMEWEGNVNT 120

Query: 121 IAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLP 180
           ++ +N V+CPRR HN  D+QVVWQD+VDPDNMTYEELLELGETVGTQSRGLSQELI+LLP
Sbjct: 121 MSRENPVDCPRRQHNGLDYQVVWQDSVDPDNMTYEELLELGETVGTQSRGLSQELISLLP 180

Query: 181 ISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY 240
           +SKYK S FSRKKSR ERCVICQMEYKRG+RRITLPCKH+YHAGCG++WLSINKACPICY
Sbjct: 181 VSKYKCSLFSRKKSRNERCVICQMEYKRGERRITLPCKHVYHAGCGSRWLSINKACPICY 240

Query: 241 TEVFGDTSK 248
           TEVFGD SK
Sbjct: 241 TEVFGDASK 247

[Close]

BLAST of Cucsa.143280 vs. TrEMBL
Match: A0A0D2TL58_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G072700 PE=4 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 4.7e-96
Identity = 172/251 (68.53%), Postives = 208/251 (82.87%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSW+  P  EV ++N+NYPYN+AGSF+EYFEGLTY+HVNFIF GASH QE+VYP + S+F
Sbjct: 1   MSWD--PHMEVQYINSNYPYNSAGSFIEYFEGLTYQHVNFIFDGASHVQESVYPSMTSSF 60

Query: 61  YKFGFSDFGSPSYYNP--PQAYRMHDHEPIIYEHRRL-ENSATQPNLQST-VNPEFEEIT 120
           YKFG SD GS SYY+     +Y +++HE  I E+RR  ENS +  N Q+  +N E+E   
Sbjct: 61  YKFGPSDSGSISYYDHRNDHSYEVNNHELCIDEYRRASENSLSGSNEQTAAMNVEWERNA 120

Query: 121 NTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIAL 180
           +  + +N V+CPRR HN+HD+QV+WQD VDPDNMTYEELLELGE+VGTQSRGL+QELI+L
Sbjct: 121 HATSLENSVDCPRRQHNAHDYQVIWQDCVDPDNMTYEELLELGESVGTQSRGLTQELISL 180

Query: 181 LPISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPI 240
           LP+SKYK S FSRKKSR ERCVICQMEYKRG+R+ITLPCKH+YHAGCGT+WLSINKACPI
Sbjct: 181 LPVSKYKCSLFSRKKSRKERCVICQMEYKRGERQITLPCKHVYHAGCGTRWLSINKACPI 240

Query: 241 CYTEVFGDTSK 248
           CYTEVFG+ SK
Sbjct: 241 CYTEVFGNGSK 249

[Close]

BLAST of Cucsa.143280 vs. TrEMBL
Match: G7JDP3_MEDTR (E3 ubiquitin ligase BIG brother-like protein OS=Medicago truncatula GN=MTR_4g065670 PE=4 SV=2)

HSP 1 Score: 359.0 bits (920), Expect = 4.7e-96
Identity = 174/250 (69.60%), Postives = 200/250 (80.00%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN  P  EVH+ N +YPYNTAGSF+EYFEGLTYEHVNFIFSGASH+QE+ YP  NSNF
Sbjct: 1   MSWN--PHMEVHYNNVSYPYNTAGSFIEYFEGLTYEHVNFIFSGASHAQESQYPA-NSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRR-LENSATQPNLQSTVNPEFEEITNTI 120
           YKFG S+  + SYY     Y ++ HEP++ E+RR  ENS+T       V+PE+ E  NT 
Sbjct: 61  YKFGLSEPENTSYYRYNHGYEVNHHEPLVDEYRRPSENSSTISEPTLVVSPEWGEDGNTD 120

Query: 121 AFDNHVECPRRHH-NSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLP 180
             DN  ECPRRHH NS+D QV+WQDN+DPDNMTYEELLELGE VGTQSRGL+QE I+LLP
Sbjct: 121 TQDNSFECPRRHHSNSNDSQVIWQDNIDPDNMTYEELLELGEAVGTQSRGLTQEQISLLP 180

Query: 181 ISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY 240
           +SK+K  FF RKKSR ERCVICQMEYKRGD+RITLPCKH+YHA CG KWL+INKACPICY
Sbjct: 181 VSKFKCGFFFRKKSRNERCVICQMEYKRGDKRITLPCKHLYHASCGNKWLTINKACPICY 240

Query: 241 TEVFGDTSKR 249
           TEVF D SK+
Sbjct: 241 TEVFADKSKQ 247

[Close]

BLAST of Cucsa.143280 vs. TAIR10
Match: AT3G63530.1 (AT3G63530.1 RING/U-box superfamily protein)

HSP 1 Score: 222.6 bits (566), Expect = 2.6e-58
Identity = 113/253 (44.66%), Postives = 163/253 (64.43%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           M+ ++ P  + H+  T +PY   GS+++++ G     +N+  +   H Q+N+Y  +N+N 
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQDNLYWTMNTNA 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPII------YEHRRLENSATQPNLQSTVNPEFEE 120
           YKFGFS   + S+Y    +Y M+DH   +      +++  + N A  P  ++TV    + 
Sbjct: 61  YKFGFSGSDNASFYG---SYDMNDHLSRMSIGRTNWDYHPMVNVADDP--ENTVARSVQ- 120

Query: 121 ITNTIAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELI 180
           I +T       EC    H+    QV WQD++DPD MTYEEL+ELGE VGT+SRGLSQELI
Sbjct: 121 IGDTDEHSEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELI 180

Query: 181 ALLPISKYKY-SFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKA 240
             LP  KYK+ S FSRK++ GERCVICQ++YK G+R++ LPCKH+YH+ C +KWLSINK 
Sbjct: 181 ETLPTKKYKFGSIFSRKRA-GERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV 240

Query: 241 CPICYTEVFGDTS 247
           CP+C +EVFG+ S
Sbjct: 241 CPVCNSEVFGEPS 246

[Close]

BLAST of Cucsa.143280 vs. TAIR10
Match: AT3G19910.1 (AT3G19910.1 RING/U-box superfamily protein)

HSP 1 Score: 115.9 bits (289), Expect = 3.5e-26
Identity = 56/113 (49.56%), Postives = 71/113 (62.83%), Query Frame = 1


Query: 134 SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSR 193
           SH  Q  W D +DPD ++YEELL LG+ VGT+SRGLS + IA LP  +YK      +   
Sbjct: 228 SHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEG--DNQNGT 287

Query: 194 GERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVFGDTS 247
            E CVIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  EV   TS
Sbjct: 288 NESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTS 337

[Close]

BLAST of Cucsa.143280 vs. TAIR10
Match: AT5G52140.1 (AT5G52140.1 RING/U-box superfamily protein)

HSP 1 Score: 106.3 bits (264), Expect = 2.8e-23
Identity = 57/119 (47.90%), Postives = 71/119 (59.66%), Query Frame = 1


Query: 132 HNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYK------YS 191
           HN  D      +N DP+NMTYEEL ELG++VG   +GLSQE I+ L   KY       Y 
Sbjct: 166 HNPTD-----DENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYC 225

Query: 192 FFSRKK--SRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTEVF 243
              +KK  +   +C IC MEY +GD+  TLPCKHIYH  C ++WL  NK C IC  EV+
Sbjct: 226 LHMKKKFVADDSQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of Cucsa.143280 vs. TAIR10
Match: AT3G47180.1 (AT3G47180.1 RING/U-box superfamily protein)

HSP 1 Score: 95.5 bits (236), Expect = 4.9e-20
Identity = 59/180 (32.78%), Postives = 92/180 (51.11%), Query Frame = 1


Query: 72  SYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIAF-------DNH 131
           S  N   A+ MHD  P +     +  S  +   +  +N  + E  ++  F       +  
Sbjct: 33  SQANSHFAHAMHDSSPSMIS---MIESDEESEDEEEINENYYEYFDSNGFGVDEDEINEF 92

Query: 132 VECPRRHHN--SHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQ-ELIALLPISK 191
           +E    + N    D  +  +D +DPD ++YEEL+ LG+ +G ++RGL+  E+   L  S 
Sbjct: 93  LEDQESNSNLEEEDDFLEEEDEIDPDQLSYEELIALGDFIGVENRGLTPIEISTCLNAST 152

Query: 192 YKYSFFSRKKSRGERCVICQMEYKRGDRRITL-PCKHIYHAGCGTKWLSINKACPICYTE 241
           Y    FS  K+  +RCV+CQME++  +  + L PC H YH+ C TKWL   K CPIC +E
Sbjct: 153 Y---VFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECITKWLETKKICPICCSE 206

[Close]

BLAST of Cucsa.143280 vs. TAIR10
Match: AT4G34040.1 (AT4G34040.1 RING/U-box superfamily protein)

HSP 1 Score: 82.0 bits (201), Expect = 5.6e-16
Identity = 42/96 (43.75%), Postives = 58/96 (60.42%), Query Frame = 1


Query: 145 VDPDNMTYEELLELGETVGTQSRGLSQELIALLPISKYKYSFFSRKKSRG-ERCVICQME 204
           +D DNM+YEELL LGE +G  S GLS+E+I L  + ++K++  +    +  E C +CQ E
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKVMKQHKHTSSAAGSHQDMEPCCVCQEE 626

Query: 205 YKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYT 240
           Y  GD   TL C H +H  C  +WL +   CPIC T
Sbjct: 627 YAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

[Close]

BLAST of Cucsa.143280 vs. NCBI nr
Match: gi|778663625|ref|XP_011660125.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER [Cucumis sativus])

HSP 1 Score: 533.9 bits (1374), Expect = 1.5e-148
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

[Close]

BLAST of Cucsa.143280 vs. NCBI nr
Match: gi|659099125|ref|XP_008450441.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Cucumis melo])

HSP 1 Score: 530.0 bits (1364), Expect = 2.2e-147
Identity = 245/248 (98.79%), Postives = 247/248 (99.60%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN+TPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF
Sbjct: 1   MSWNNTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120
           YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA
Sbjct: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQSTVNPEFEEITNTIA 120

Query: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180
           FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS
Sbjct: 121 FDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLPIS 180

Query: 181 KYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240
           KYKY FFSRKKSRG+RCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE
Sbjct: 181 KYKYGFFSRKKSRGDRCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICYTE 240

Query: 241 VFGDTSKR 249
           VFGDTSKR
Sbjct: 241 VFGDTSKR 248

[Close]

BLAST of Cucsa.143280 vs. NCBI nr
Match: gi|1009118666|ref|XP_015875978.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Ziziphus jujuba])

HSP 1 Score: 374.8 bits (961), Expect = 1.2e-100
Identity = 182/250 (72.80%), Postives = 206/250 (82.40%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNT-NYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSN 60
           MSWN  P  EVH+VN  +YPY+TAGSF+EY EGLTYEHVNFIFSGASH+QE+VYP +N++
Sbjct: 1   MSWN--PHMEVHYVNNASYPYSTAGSFMEYVEGLTYEHVNFIFSGASHAQESVYPSMNTS 60

Query: 61  FYKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRR-LENSATQPNLQSTVNPEFEEITNT 120
           FYKFG S+ GS SYY+    Y +HDH+    E+RR +ENS T  N Q+       E  N+
Sbjct: 61  FYKFGLSEPGSTSYYDFGHVYEVHDHDLRSEEYRRPMENSPTMTNNQTAAPNSEWEGNNS 120

Query: 121 IAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLP 180
              D  VECPRRHH+SHD+QV+WQDNVDPDNMTYEELLELGETVGTQSRGLSQELI+LLP
Sbjct: 121 NTVDIPVECPRRHHSSHDYQVIWQDNVDPDNMTYEELLELGETVGTQSRGLSQELISLLP 180

Query: 181 ISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY 240
           ISKYK S FSRKKSR +RCVICQMEYKRGDRRITLPCKHIYHAGCGT+WLSINKACPICY
Sbjct: 181 ISKYKCSLFSRKKSRHDRCVICQMEYKRGDRRITLPCKHIYHAGCGTRWLSINKACPICY 240

Query: 241 TEVFGDTSKR 249
            EVFGD SK+
Sbjct: 241 KEVFGDASKQ 248

[Close]

BLAST of Cucsa.143280 vs. NCBI nr
Match: gi|568832758|ref|XP_006470593.1| (PREDICTED: E3 ubiquitin ligase BIG BROTHER [Citrus sinensis])

HSP 1 Score: 370.2 bits (949), Expect = 2.9e-99
Identity = 174/249 (69.88%), Postives = 204/249 (81.93%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN  P  EVH+ NT+YPYN+AGSF+EYFEGLTYEHVNFIF GASH QE+VYP  +++F
Sbjct: 1   MSWN--PHIEVHYYNTSYPYNSAGSFMEYFEGLTYEHVNFIFDGASHVQESVYPSTHTSF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRLENSATQPNLQST--VNPEFEEITNT 120
           YKFG SD+GS SYY+    Y ++ HEP   E+RR   + ++ N + T  VN   E   NT
Sbjct: 61  YKFGLSDYGSNSYYDYGHPYEVNYHEPRNDEYRRPSENTSRSNNEQTAAVNAVPERNMNT 120

Query: 121 IAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLP 180
              ++ +ECPRRH N+H+ QVVWQDN+DPD MTYEELLELGETVGTQSRGLSQELI+LLP
Sbjct: 121 TTRESPIECPRRHQNTHEHQVVWQDNIDPDQMTYEELLELGETVGTQSRGLSQELISLLP 180

Query: 181 ISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY 240
           ISKYK  FF+RK+SR ERCVICQMEYKRGDRR+TLPCKH+YHAGCGT+WLSINKACPICY
Sbjct: 181 ISKYKCGFFTRKRSRNERCVICQMEYKRGDRRMTLPCKHVYHAGCGTRWLSINKACPICY 240

Query: 241 TEVFGDTSK 248
           TEVFGD+SK
Sbjct: 241 TEVFGDSSK 247

[Close]

BLAST of Cucsa.143280 vs. NCBI nr
Match: gi|590583795|ref|XP_007014997.1| (RING/U-box superfamily protein isoform 1 [Theobroma cacao])

HSP 1 Score: 369.8 bits (948), Expect = 3.8e-99
Identity = 175/249 (70.28%), Postives = 209/249 (83.94%), Query Frame = 1


Query: 1   MSWNSTPQTEVHHVNTNYPYNTAGSFLEYFEGLTYEHVNFIFSGASHSQENVYPPLNSNF 60
           MSWN  P  EV ++N++YPYN+AGSF+EYFEGLTY+HVNFIF GASH QE+VYP + S+F
Sbjct: 1   MSWN--PHMEVQYINSSYPYNSAGSFMEYFEGLTYQHVNFIFDGASHVQESVYPSMTSSF 60

Query: 61  YKFGFSDFGSPSYYNPPQAYRMHDHEPIIYEHRRL-ENSATQPNLQST-VNPEFEEITNT 120
           YKFG SD G+ SYY+    Y +++HE  + E+RR  ENS++  N Q+  +N E+E   NT
Sbjct: 61  YKFGLSDSGNISYYDHGHNYEVNNHELCVDEYRRASENSSSMSNEQTAAMNMEWEGNVNT 120

Query: 121 IAFDNHVECPRRHHNSHDFQVVWQDNVDPDNMTYEELLELGETVGTQSRGLSQELIALLP 180
           ++ +N V+CPRR HN  D+QVVWQD+VDPDNMTYEELLELGETVGTQSRGLSQELI+LLP
Sbjct: 121 MSRENPVDCPRRQHNGLDYQVVWQDSVDPDNMTYEELLELGETVGTQSRGLSQELISLLP 180

Query: 181 ISKYKYSFFSRKKSRGERCVICQMEYKRGDRRITLPCKHIYHAGCGTKWLSINKACPICY 240
           +SKYK S FSRKKSR ERCVICQMEYKRG+RRITLPCKH+YHAGCG++WLSINKACPICY
Sbjct: 181 VSKYKCSLFSRKKSRNERCVICQMEYKRGERRITLPCKHVYHAGCGSRWLSINKACPICY 240

Query: 241 TEVFGDTSK 248
           TEVFGD SK
Sbjct: 241 TEVFGDASK 247

The following BLAST results are available for this feature:

BLAST of Cucsa.143280 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BB_ARATH	4.7e-57	44.66	E3 ubiquitin ligase BIG BROTHER OS=Arabidopsis thaliana GN=BB PE=1 SV=1	[more]
BBR_ARATH	6.2e-25	49.56	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana GN=BBR PE=2 SV=1	[more]
MBR1_ARATH	4.5e-15	45.00	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana GN=MBR1 PE=1 SV=1	[more]
MBR2_ARATH	9.9e-15	43.75	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana GN=MBR2 PE=1 SV=1	[more]
HIP1_ORYSJ	1.7e-14	45.74	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica GN=HIP...	[more]

BLAST of Cucsa.143280 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LX98_CUCSA	1.1e-148	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G613470 PE=4 SV=1	[more]
A0A067F1T9_CITSI	2.0e-99	69.88	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g025212mg PE=4 SV=1	[more]
A0A061GTY3_THECC	2.7e-99	70.28	RING/U-box superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_040623 PE=4 S...	[more]
A0A0D2TL58_GOSRA	4.7e-96	68.53	Uncharacterized protein OS=Gossypium raimondii GN=B456_009G072700 PE=4 SV=1	[more]
G7JDP3_MEDTR	4.7e-96	69.60	E3 ubiquitin ligase BIG brother-like protein OS=Medicago truncatula GN=MTR_4g065...	[more]

BLAST of Cucsa.143280 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G63530.1	2.6e-58	44.66	RING/U-box superfamily protein	[more]
AT3G19910.1	3.5e-26	49.56	RING/U-box superfamily protein	[more]
AT5G52140.1	2.8e-23	47.90	RING/U-box superfamily protein	[more]
AT3G47180.1	4.9e-20	32.78	RING/U-box superfamily protein	[more]
AT4G34040.1	5.6e-16	43.75	RING/U-box superfamily protein	[more]

BLAST of Cucsa.143280 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778663625\|ref\|XP_011660125.1\|	1.5e-148	100.00	PREDICTED: E3 ubiquitin ligase BIG BROTHER [Cucumis sativus]	[more]
gi\|659099125\|ref\|XP_008450441.1\|	2.2e-147	98.79	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Cucumis melo]	[more]
gi\|1009118666\|ref\|XP_015875978.1\|	1.2e-100	72.80	PREDICTED: E3 ubiquitin ligase BIG BROTHER-like [Ziziphus jujuba]	[more]
gi\|568832758\|ref\|XP_006470593.1\|	2.9e-99	69.88	PREDICTED: E3 ubiquitin ligase BIG BROTHER [Citrus sinensis]	[more]
gi\|590583795\|ref\|XP_007014997.1\|	3.8e-99	70.28	RING/U-box superfamily protein isoform 1 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding

Vocabulary: **Biological Process**
Term	Definition
GO:0016567	protein ubiquitination
GO:0046621	negative regulation of organ growth
GO:0016567	protein ubiquitination
GO:0046621	negative regulation of organ growth

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.143280.2	Cucsa.143280.2	mRNA
Cucsa.143280.1	Cucsa.143280.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 196..237 score: 1.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 197..237 score: 2.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 197..237 score: 10
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 193..241 score: 1.4
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 38..80 score: 5.3E-84coord: 97..244 score: 5.3
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 196..242 score: 1.06

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.143280	Csa1G613470	Cucumber (Chinese Long) v2	cgycuB205
Cucsa.143280	MELO3C014603	Melon (DHL92) v3.5.1	cgymeB239
Cucsa.143280	ClCG03G013370	Watermelon (Charleston Gray)	cgywcgB242
Cucsa.143280	CSPI01G30250	Wild cucumber (PI 183967)	cgycpiB208
Cucsa.143280	CmaCh10G009400	Cucurbita maxima (Rimu)	cgycmaB0395
Cucsa.143280	CmaCh11G008380	Cucurbita maxima (Rimu)	cgycmaB0396
Cucsa.143280	CmaCh20G000770	Cucurbita maxima (Rimu)	cgycmaB0398
Cucsa.143280	CmoCh11G008490	Cucurbita moschata (Rifu)	cgycmoB0396
Cucsa.143280	CmoCh10G009850	Cucurbita moschata (Rifu)	cgycmoB0395
Cucsa.143280	CmoCh02G012810	Cucurbita moschata (Rifu)	cgycmoB0399
Cucsa.143280	Lsi06G005150	Bottle gourd (USVL1VR-Ls)	cgylsiB230
Cucsa.143280	Cp4.1LG05g05080	Cucurbita pepo (Zucchini)	cgycpeB0386
Cucsa.143280	Cp4.1LG18g01600	Cucurbita pepo (Zucchini)	cgycpeB0382
Cucsa.143280	Cp4.1LG04g11960	Cucurbita pepo (Zucchini)	cgycpeB0385
Cucsa.143280	MELO3C029471.2	Melon (DHL92) v3.6.1	cgymedB235
Cucsa.143280	CsaV3_1G042670	Cucumber (Chinese Long) v3	cgycucB213
Cucsa.143280	Cla97C03G063580	Watermelon (97103) v2	cgywmbB241
Cucsa.143280	Carg17978	Silver-seed gourd	carcgyB0212
Cucsa.143280	Carg16949	Silver-seed gourd	carcgyB0539

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.143280	Cucumber (Chinese Long) v3	cgycucB216
Cucsa.143280	Wax gourd	cgywgoB277
Cucsa.143280	Wax gourd	cgywgoB278
Cucsa.143280	Wax gourd	cgywgoB281
Cucsa.143280	Wax gourd	cgywgoB282
Cucsa.143280	Cucumber (Gy14) v1	cgycgyB010
Cucsa.143280	Cucurbita maxima (Rimu)	cgycmaB0397
Cucsa.143280	Cucurbita maxima (Rimu)	cgycmaB0399
Cucsa.143280	Cucurbita moschata (Rifu)	cgycmoB0397
Cucsa.143280	Cucurbita moschata (Rifu)	cgycmoB0398
Cucsa.143280	Wild cucumber (PI 183967)	cgycpiB211
Cucsa.143280	Cucumber (Chinese Long) v2	cgycuB208
Cucsa.143280	Melon (DHL92) v3.5.1	cgymeB237
Cucsa.143280	Melon (DHL92) v3.5.1	cgymeB238
Cucsa.143280	Watermelon (97103) v1	cgywmB251
Cucsa.143280	Bottle gourd (USVL1VR-Ls)	cgylsiB228
Cucsa.143280	Melon (DHL92) v3.6.1	cgymedB234
Cucsa.143280	Silver-seed gourd	carcgyB0599
Cucsa.143280	Silver-seed gourd	carcgyB1039